WO2024130534A1 - Système de circulation de liquide et son dispositif associé, et procédé de nettoyage - Google Patents

Système de circulation de liquide et son dispositif associé, et procédé de nettoyage Download PDF

Info

Publication number
WO2024130534A1
WO2024130534A1 PCT/CN2022/140259 CN2022140259W WO2024130534A1 WO 2024130534 A1 WO2024130534 A1 WO 2024130534A1 CN 2022140259 W CN2022140259 W CN 2022140259W WO 2024130534 A1 WO2024130534 A1 WO 2024130534A1
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
fluid
cavity
liquid
needle
Prior art date
Application number
PCT/CN2022/140259
Other languages
English (en)
Chinese (zh)
Inventor
陆灏
陈泽华
牛子华
邢楚填
Original Assignee
深圳华大智造科技股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 深圳华大智造科技股份有限公司 filed Critical 深圳华大智造科技股份有限公司
Priority to PCT/CN2022/140259 priority Critical patent/WO2024130534A1/fr
Publication of WO2024130534A1 publication Critical patent/WO2024130534A1/fr

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
    • B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
    • B08B9/027—Cleaning the internal surfaces; Removal of blockages
    • B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Definitions

  • the present application relates to the field of fluid control technology, and in particular to a liquid circuit system, a gene sequencer, a blood cell analyzer, a chemiluminescent immunoassay analyzer, a flow cytometer, a detection device and a cleaning method.
  • the operation of detection instruments mostly depends on samples and various buffers, reactant solutions, enzymes, eluents, etc. These substances usually reach the reaction chamber from the reagent kit through reagent needles and pipes. After the detection task is completed, if the various reagents remaining in the reagent needles and pipes are not cleaned, it is easy to cause crystal blockage, protein adsorption, bacterial growth, sample cross-contamination, affecting the next test and other adverse consequences.
  • the present application proposes a liquid circuit system, a gene sequencer, a blood cell analyzer, a chemiluminescent immunoassay analyzer, a flow cytometer, a detection device and a cleaning method to facilitate the cleaning of the liquid circuit system.
  • the present application provides a fluid circuit system, comprising:
  • a cavity structure capable of relative movement with the reagent needle assembly to be in contact with or out of contact with the reagent needle assembly;
  • a flow cell having one end connected to the inner cavity of the reagent needle assembly directly or through a pipeline;
  • a fluid power component is connected to the other end of the flow pool directly or through a pipeline, and is used to drive the fluid in the flow pool into the cavity structure, or to drive the fluid in the cavity structure into the flow pool, and is used to drive the fluid in the reagent needle into the flow pool when the reagent needle assembly is not in contact with the cavity structure.
  • the reagent needle assembly includes at least one reagent needle
  • the cavity structure includes at least one cavity
  • the reagent needles and the cavities are arranged in a one-to-one correspondence.
  • the number of the reagent needles and the number of the cavities are at least 2;
  • the fluid circuit system further includes a fluid control component, which is respectively connected to the flow pool and each of the reagent needles directly or through a pipeline, and the fluid control component is used to switch the flow pool to be connected to different reagent needles.
  • the reagent needles in the reagent needle assembly are arranged in parallel and at intervals in a straight line;
  • the pipelines of the fluid control component connecting the reagent needles and the flow pool are integrated on the multi-manifold block.
  • the multi-manifold block is provided with an air hole connected to the fluid control component for inputting air.
  • the number of the reagent needles and the number of the cavities are at least 3;
  • At least one of the cavities is used to store cleaning fluid and can be directly connected to the fluid power member through the reagent needle; at least one of the cavities is used to store reagent and can be directly connected to the fluid control member through the reagent needle; at least one of the cavities is used to store waste fluid and can be directly connected to the fluid power member;
  • the flow cell is used to store samples to be reacted
  • the fluid control component switches to the flow pool to communicate with the cavity containing the reagent, and the fluid power component drives the reagent into the flow pool for reaction;
  • the fluid power component is connected to the flow pool and the cavity for storing waste liquid, so as to drive the waste liquid generated by the reaction into the cavity for storing waste liquid through the fluid power component;
  • the fluid power component When cleaning the reagent needle corresponding to the reagent participating in the reaction, the fluid power component is connected to the flow pool and the cavity for storing the cleaning liquid, so that the cleaning liquid is driven into the flow pool through the fluid power component, and the cleaning liquid is driven to flow through the reagent needle into the corresponding cavity to complete reverse cleaning;
  • the reagent needle When it is necessary to empty the reagent needle after reverse cleaning, the reagent needle is not in contact with the cavity, and the liquid in the reagent needle is driven to the flow pool through the fluid power member to empty the liquid in the reagent needle.
  • the fluid circuit system further includes a fluid reservoir module
  • the fluid reservoir module is connected to the fluid power component directly or through a pipeline, and is used to store cleaning fluid or waste fluid.
  • the fluid reservoir module is used to store cleaning fluid
  • the number of the reagent needles and the cavities is at least 2, and each cavity can be directly connected to the fluid control component through the reagent needle;
  • At least one of the cavities is used to store reagents, and at least one of the cavities is used to store waste liquid;
  • the flow cell is used to store samples to be reacted
  • the fluid control component switches to the flow pool to communicate with the cavity containing the reagent, and the fluid power component drives the reagent into the flow pool for reaction;
  • the fluid control component switches to the flow pool to communicate with the cavity for holding the waste liquid, and the fluid power component drives the waste liquid generated by the reaction into the cavity for holding the waste liquid;
  • the fluid control component switches to the fluid reservoir module, the flow pool and the reagent needle to be cleaned, so that the cleaning liquid in the fluid reservoir module flows through the flow pool and the reagent needle in sequence through the fluid power component to drive the cleaning liquid into the corresponding cavity to complete reverse cleaning;
  • the reagent needle When the reagent needle after reverse cleaning needs to be emptied, the reagent needle is placed in a non-contact manner with the cavity, and the liquid in the reagent needle is driven to the flow pool through the fluid power member to empty the liquid in the reagent needle.
  • the fluid reservoir module is used to store cleaning fluid
  • the number of the reagent needles and the number of the cavities are at least 2;
  • At least one of the cavities is used to store reagents and is directly connected to the fluid control component through the reagent needle; at least one of the cavities is used to store waste liquid and is directly connected to the fluid power component through the reagent needle;
  • the flow cell is used to store samples to be reacted
  • the fluid control component switches to the flow pool to communicate with the cavity containing the reagent, and the fluid power component drives the reagent into the flow pool for reaction;
  • the fluid power element is connected to the flow pool and the cavity for holding the waste liquid, so as to drive the waste liquid generated by the reaction directly into the cavity for holding the waste liquid;
  • the fluid control component switches to the fluid reservoir module, the flow pool and the reagent needle to be cleaned, so that the cleaning liquid in the fluid reservoir module flows through the flow pool and the reagent needle in sequence through the fluid power component to drive the cleaning liquid into the corresponding cavity to complete reverse cleaning;
  • the reagent needle When the reagent needle after reverse cleaning needs to be emptied, the reagent needle is placed in a non-contact manner with the cavity, and the liquid in the reagent needle is driven to the flow pool through the fluid power member to empty the liquid in the reagent needle.
  • the fluid reservoir module is used to store waste liquid
  • the number of the reagent needles and the cavities is at least 2, and each cavity can be directly connected to the fluid control component through the reagent needle;
  • At least one of the cavities is used to store reagents, and at least one of the cavities is used to store cleaning fluid;
  • the flow cell is used to store samples to be reacted
  • the fluid control component switches to the flow pool to communicate with the cavity containing the reagent, and the fluid power component drives the reagent into the flow pool for reaction;
  • the fluid power component is directly connected to the flow cell and the fluid reservoir module to drive the waste liquid generated by the reaction into the fluid reservoir module;
  • the fluid control component switches to the flow pool to communicate with the reagent needle to be cleaned, and the fluid power component drives the cleaning liquid into the flow pool, and the cleaning liquid flows through the reagent needle into the corresponding cavity to complete reverse cleaning;
  • the reagent needle When the reagent needle after reverse cleaning needs to be emptied, the reagent needle is out of contact with the cavity, and the liquid in the reagent needle is driven to the flow pool and/or the fluid reservoir module by the fluid power member to empty the liquid in the reagent needle.
  • the fluid reservoir module is used to store waste liquid
  • the number of the reagent needles and the number of the cavities are at least 2;
  • At least one of the cavities is used to store a reagent, and can be directly connected to the fluid control component through the reagent needle; at least one of the cavities is used to store a cleaning liquid, and can be directly connected to the fluid power component through the reagent needle;
  • the flow cell is used to store samples to be reacted
  • the fluid control component switches to the flow pool to communicate with the cavity containing the reagent, and the fluid power component drives the reagent into the flow pool for reaction;
  • the fluid power component is directly connected to the flow cell and the fluid reservoir module to drive the waste liquid generated by the reaction into the fluid reservoir module;
  • the fluid power component When cleaning the reagent needle corresponding to the reagent participating in the reaction, the fluid power component is connected to the flow pool and the cavity containing the cleaning liquid to drive the cleaning liquid into the flow pool, and the cleaning liquid flows through the reagent needle into the corresponding cavity to complete reverse cleaning;
  • the reagent needle When the reagent needle after reverse cleaning needs to be emptied, the reagent needle is out of contact with the cavity, and the liquid in the reagent needle is driven to the flow pool and/or the fluid reservoir module by the fluid power member to empty the liquid in the reagent needle.
  • the fluid reservoir module includes a waste liquid reservoir module and a cleaning liquid reservoir module respectively connected to the fluid power member;
  • the waste liquid storage module is used to hold waste liquid, and the cleaning liquid storage module is used to hold cleaning liquid;
  • the number of the reagent needle and the number of the cavity are at least 1;
  • At least one of the cavities is used to store a reagent and is directly connected to the fluid control member through the reagent needle;
  • the flow cell is used to store samples to be reacted
  • the fluid control component switches to the flow pool to communicate with the cavity containing the reagent, and the fluid power component drives the reagent into the flow pool for reaction;
  • the fluid power component is connected to the flow pool and the waste liquid reservoir module to directly drive the waste liquid generated by the reaction into the waste liquid reservoir module;
  • the fluid power component When cleaning the reagent needle corresponding to the reagent participating in the reaction, the fluid power component is connected to the flow pool and the cleaning liquid reservoir module, so that the cleaning liquid in the fluid reservoir module flows through the flow pool and the reagent needle in sequence through the fluid power component to drive the cleaning liquid into the corresponding cavity to complete reverse cleaning;
  • the reagent needle When the reagent needle after reverse cleaning needs to be emptied, the reagent needle is placed in a non-contact manner with the cavity, and the liquid in the reagent needle is driven to the flow pool through the fluid power member to empty the liquid in the reagent needle.
  • the fluid control component is a multi-channel rotary valve, a solenoid valve group, a pinch valve group or a slider valve.
  • the fluid circuit system further includes a fluid storage module
  • the fluid storage module is in communication with the cavity structure and is used for containing liquid.
  • a liquid containing cavity is provided on the fluid storage module, and the liquid containing cavity is provided in a one-to-one correspondence with and communicated with the cavity in the cavity structure.
  • the fluid storage module includes a box body having an inner cavity, and the inner cavity of the box body is the liquid holding cavity;
  • the fluid storage module comprises a box body having an inner cavity and a partition plate arranged in the box body, wherein the partition plate divides the inner cavity of the box body into at least two liquid containing chambers.
  • the cavity structure includes a side channel structure and a sealing strip integrated on the side wall of the fluid storage module;
  • the side channel structure is provided with a channel, the channel is arranged in one-to-one correspondence with the liquid holding cavity, and one end of the channel is communicated with the liquid holding cavity, and the sealing strip seals the other end of the channel to form the cavity;
  • the reagent needle can pierce the sealing strip and enter the cavity.
  • At least a portion of a side of the flow channel facing away from the fluid storage module is open;
  • the cavity structure further comprises a side sealing film and a side cover, wherein the side sealing film seals the opening on the side of the flow channel, and the side cover is respectively pressed with the side sealing film and the sealing strip and is tightly connected to the side flow channel structure.
  • the side cover includes a first side cover portion and a second side cover portion connected to the first side cover portion;
  • the first side cover part covers the opening on the side of the flow channel, and the second side cover part is provided with a puncture hole coaxially arranged with the flow channel.
  • a plug is provided at the end of the sealing strip facing the flow channel, and the plug blocks the end of the flow channel away from the liquid containing cavity;
  • One of the end of the sealing strip facing away from the flow channel and the second side cover portion is provided with a positioning groove, and the other is provided with a positioning protrusion which is limited by the positioning groove.
  • the reagent needle includes a needle body and a needle tip
  • the needle body is a tubular structure with two open ends, the needle tip is sealed and connected to one end of the needle body, and a connecting hole communicating with the inner cavity of the needle body is provided at the connection between the needle body and the needle tip.
  • the connecting hole is arranged obliquely with respect to the axis of the needle body.
  • the fluid power component is a syringe pump, a plunger pump, a diaphragm pump, a piezoelectric pump or a gear pump.
  • the present application provides a gene sequencer, including a sequencer body and a liquid path system as described in any one of the above;
  • the liquid path system is installed on the sequencer body.
  • the present application provides a blood cell analyzer, including a blood cell analyzer body and a liquid path system as described in any one of the above items;
  • the liquid path system is installed on the hemacytometer body.
  • the present application provides a chemiluminescent immunoassay analyzer, comprising an analyzer body and a liquid path system as described in any one of the above;
  • the liquid path system is installed on the analyzer body.
  • the present application provides a flow cytometer, comprising a cytometer body and a fluid path system as described in any one of the above;
  • the fluid path system is installed on the cytometer body.
  • the present application provides a detection device, comprising a device body and a fluid path system as described in any one of the above;
  • the fluid system is installed on the equipment body.
  • the present application provides a sorting method, comprising:
  • the reagent needle assembly is brought into contact with the cavity structure, and the fluid power component is activated to drive the reagent into the flow cell to react with the sample to be reacted;
  • the waste liquid generated by the reaction is positively pumped out from the flow cell through the fluid power member;
  • the cleaning fluid is driven into the reagent needle and the cavity structure by the fluid power member to reversely clean the reagent needle;
  • the reagent needle assembly is placed in a non-contact manner with the cavity structure, and the fluid in the reagent needle is driven into the flow pool through the fluid power member.
  • the present application provides a liquid circuit system.
  • the reagent needle assembly When in use, taking the injection of the sample to be reacted into the flow cell and the injection of the reagent into the cavity structure as an example, the reagent needle assembly is contacted with the cavity structure, and the fluid power part is started to drive the reagent into the flow cell to react with the sample to be reacted; after the reaction is completed, the waste liquid generated by the reaction is positively extracted from the flow cell by the fluid power part; the cleaning liquid is driven into the reagent needle and the cavity structure by the fluid power part, and the reagent needle is reversely cleaned; the reagent needle assembly is not in contact with the cavity structure, and the fluid in the reagent needle is driven into the flow cell by the fluid power part to empty the reagent needle. It can be seen that the present application does not need to remove the reagent needle and put it into the cleaning tank for cleaning, and the existing liquid circuit system can be continued to be used to complete the cleaning of the entire liquid circuit, which effectively reduces the cost.
  • FIG1 is a schematic diagram of the structure of a fluid circuit system provided in an embodiment of the present application.
  • FIG2 is a schematic diagram of a partial front view of the structure of the fluid path system provided by the present application during forward flow;
  • FIG3 is a schematic diagram of a partial front view of the structure of the liquid circuit system provided by the present application when reverse flow occurs;
  • FIG4 is a schematic diagram of an exploded structure of a cavity structure and a fluid storage module provided by the present application assembled together;
  • FIG5 is a schematic diagram of a partially enlarged structure of FIG4 ;
  • FIG6 is a schematic diagram of the three-dimensional structure of the reagent needle provided in the present application.
  • FIG7 is a schematic cross-sectional view of the reagent needle provided in the present application.
  • FIG8 is a schematic cross-sectional view of the structure of the reagent needle in contact with the cavity provided by the present application;
  • FIG9 is a schematic cross-sectional view of a vortex formed when liquid flows through a reagent needle and enters a cavity provided by the present application;
  • FIG10 is a schematic diagram of the structure of the fluid circuit system provided in Example 1 of the present application.
  • FIG11 is a schematic diagram of the structure of the liquid circuit system provided in Embodiments 3 and 5 of the present application.
  • FIG12 is a schematic diagram of the structure of the liquid circuit system provided in Embodiments 2 and 4 of the present application.
  • FIG. 13 is a schematic diagram of the structure of the liquid circuit system provided in Example 6 of the present application.
  • Fluid system 100 reagent needle 101a, reagent needle assembly 101, cavity 102a, cavity structure 102, flow cell 103, fluid power part 104, fluid control part 105, manifold block 106, fluid reservoir module 107, waste liquid reservoir module 107a, cleaning liquid reservoir module 107b, fluid storage module 108, liquid holding cavity 108a, box body 108b, partition 108c, side channel structure 102b, channel 102b-1, sealing strip 102c, side sealing film 102d, side cover 102e, first side cover part 102e-1, first side cover part 102e-2, second side cover part 102e-3, second side cover part 102e-4, second side cover part 102e-5, second side cover part 102e-6, second side cover part 102e-7, second side cover part 102e-8, second side cover part 102e-9, second side cover part 102e-1 Two side covers 102e-2, puncture hole 102e-2a, plug 102c-1, positioning protrusion 102c-2, needle body 101a
  • the liquid circuit system 100 in the embodiment of the present application specifically, the liquid circuit system 100 includes a reagent needle assembly 101, a cavity structure 102, a flow pool 103 and a fluid power part 104, wherein the cavity structure 102 can move relative to the reagent needle assembly 101 to be in contact or non-contact with the reagent needle assembly 101.
  • One end of the flow cell 103 is directly connected to the inner cavity of the reagent needle assembly 101 .
  • one end of the flow cell 103 may also be connected to the inner cavity of the reagent needle assembly 101 through the pipeline 109 .
  • the fluid power component 104 is directly connected to the other end of the flow cell 103, and of course, can also be connected through a pipeline 109.
  • the fluid power component 104 is a power component such as a syringe pump, a plunger pump, a diaphragm pump, a piezoelectric pump or a gear pump.
  • the fluid power member 104 is used to transport the fluid in the flow pool 103 to the cavity structure 102, or to transport the fluid in the cavity structure 102 to the flow pool 103, and is used to drive the fluid in the reagent needle 101a to the flow pool 103 when the reagent needle assembly 101 is not in contact with the cavity structure 102.
  • the fluid can be a reagent or a cleaning fluid.
  • the reagent needle assembly 101 is brought into contact with the cavity structure 102, and the fluid power part 104 is started to drive the reagent into the flow pool 103 to react with the sample to be reacted; after the reaction is completed, the waste liquid generated by the reaction is positively extracted from the flow pool 103 through the fluid power part 104; the cleaning liquid is transported to the reagent needle 101a and the cavity structure 102 through the fluid power part 104, and the inner cavity of the reagent needle 101a is reversely cleaned, and the cleaning liquid entering the cavity structure 102 can clean the outer wall of the reagent needle 101a; the reagent needle assembly 101 is not in contact with the cavity structure 102, and the fluid in the reagent needle 101a is driven into the flow pool 103 through the fluid power part 104 to empty the reagent needle 101a. It can be seen that the present application can efficiently and thoroughly clean the entire liquid path system 100
  • the reagent needle assembly 101 includes at least one reagent needle 101a
  • the cavity structure 102 includes at least one cavity 102a
  • the reagent needle 101a and the cavity 102a are arranged in a one-to-one correspondence.
  • the number of reagent needles 101a and cavities 102a is at least 2, and the liquid path system 100 also includes a fluid control component 105, which is directly connected to the flow pool 103 and each reagent needle 101a.
  • the fluid control component 105 can also be connected to the flow pool 103 and each reagent needle 101a through a pipeline 109. The fluid control component 105 is used to switch the flow pool 103 to communicate with different reagent needles 101a, thereby achieving communication with different cavities 102a.
  • the fluid control member 105 is a multi-channel rotary valve, a solenoid valve group, a pinch valve group or a slide valve.
  • the reagent needles 101 a in the reagent needle assembly 101 are arranged in parallel and spaced apart in a straight line, and the pipelines 109 of the fluid control component 105 connecting each reagent needle 101 a and the flow pool 103 are integrated on the multi-manifold block 106 , thereby improving the integration of the liquid system 100 .
  • fluid control component 105 and the flow cell 103 may also be integrated on the manifold block 106 at the same time to further improve the integration of the fluid system 100 .
  • an air hole connected to the fluid control component 105 for inputting air is provided on the manifold block 106.
  • air is first sucked into the flow pool 103 through the fluid power component 104 in the forward direction, and then the air is pushed to the reagent needle 101a through the fluid power component 104 in the reverse cleaning, so as to pre-treat the reagent needle 101a and the pipeline 109 through which it flows, thereby reducing the amount of cleaning fluid used during cleaning.
  • the fluid path system 100 further includes a fluid storage module 108 .
  • the fluid storage module 108 is in communication with the cavity structure 102 and is used to hold liquid.
  • the fluid storage module 108 is provided with a liquid holding cavity 108a, and the liquid holding cavity 108a is arranged in one-to-one correspondence with and communicates with the cavity 102a in the cavity structure 102.
  • the liquid holding cavity 108a is not limited to being arranged in one-to-one correspondence with the cavity 102a in the cavity structure 102, and can also be arranged in other ways according to actual needs, for example, two liquid holding cavities 108a correspond to and communicate with one cavity 102a in the cavity structure 102; of course, it can also be arranged that one liquid holding cavity 108a corresponds to two cavities 102a in the cavity structure 102, etc.
  • the fluid storage module 108 includes a box body 108 b having an inner cavity.
  • the inner cavity of the box body 108 b is the liquid holding cavity 108 a .
  • the fluid storage module 108 includes a box body 108b and a partition 108c, wherein the box body 108b has an inner cavity, and the partition 108c is arranged in the box body 108b to separate the inner cavity of the box body 108b into at least two liquid holding cavities 108a. It should be noted that the distance between the partitions 108c is specifically set according to the volume size of the liquid holding cavity 108a.
  • the cavity structure 102 includes a side channel structure 102b and a sealing strip 102c, wherein the side channel structure 102b is integrated on the side wall of the fluid storage module 108, specifically, integrated on the side wall of the box body 108b.
  • a flow channel 102b-1 is opened on the side flow channel structure 102b. As shown in Figure 5, the flow channel 102b-1 is arranged one-to-one corresponding to the liquid holding cavity 108a, and one end is connected to the liquid holding cavity 108a.
  • the sealing strip 102c seals the other end of the flow channel 102b-1 to form a sealed cavity 102a.
  • the reagent needle 101a can pierce the sealing strip 102c and enter the cavity 102a.
  • the sealing strip 102c will exert a shear force on the outer wall of the reagent needle 101a, scraping away the residual liquid on the outer wall of the reagent needle 101a, thereby enhancing the cleaning effect and accelerating the drying of the outer wall of the reagent needle 101a.
  • the cavity structure 102 also includes a side sealing film 102d and a side cover 102e.
  • the side sealing film 102d seals the opening on the side of the flow channel 102b-1.
  • the side cover 102e is respectively pressed with the side sealing film 102d and the sealing strip 102c and is fastened to the side flow channel structure 102b.
  • the side cover 102e is detachably connected to the side flow channel structure 102b by fasteners such as screws, so as to facilitate disassembly and replacement.
  • the side cover 102e seals the side opening of the flow channel 102b-1, and on the other hand, it is pressed onto the sealing strip 102c to ensure that the sealing strip 102c does not fall off when the reagent needle 101a moves relative to the sealing strip 102c.
  • the side cover 102e includes a first side cover portion 102e-1 and a second side cover portion 102e-2 connected to the first side cover plate 102e-1.
  • the first side cover portion 102e-1 and the second side cover portion 102e-2 are integrally formed and connected. Of course, they can also be bonded or connected by other means.
  • the first side cover portion 102e-1 covers the opening on the side of the flow channel 102b-1, and the second side cover portion 102e-2 is provided with a puncture hole 102e-2a corresponding to the flow channel 102b-1.
  • a plug 102c-1 is provided at the end of the sealing strip 102c facing the flow channel 102b-1, and the plug 102c-1 is inserted into the end of the flow channel 102b-1 away from the liquid containing cavity 108a to block the flow channel 102b-1, thereby achieving a stable seal of the flow channel 102b-1.
  • the end of the sealing strip 102c away from the flow channel 102b-1 is provided with a positioning groove
  • the second side cover portion 102e-2 is provided with a positioning protrusion 102c-2 limited by the positioning groove, as shown in Figure 8.
  • the second side cover portion 102e-2 can also be provided with a positioning groove
  • the end of the sealing strip 102c away from the flow channel 102b-1 is provided with a positioning protrusion 102c-2 limited by the positioning groove.
  • the reagent needle 101a includes a needle body 101a-1 and a needle tip 101a-2, wherein the needle body 101a-1 is a tubular structure with two ends open, the needle tip 101a-2 is sealed and connected to one end of the needle body 101a-1, and a connecting hole 101a-3 connected to the inner cavity of the needle body 101a-1 is provided at the connection between the needle body 101a-1 and the needle tip 101a-2.
  • the cleaning liquid is ejected from the connecting hole 101a-3 on the side of the reagent needle 101a to form a jet, and after impacting the inner surface of the flow channel 102b-1, a vortex shown in Fig. 9 will be formed below the liquid surface, and the vortex will further send the cleaning liquid to the outer wall surface of the reagent needle 101a to clean the reagent remaining on the outer wall.
  • the connecting hole 101a-3 is inclined or perpendicular to the axis of the needle body 101a-1. Specifically, taking the connecting hole 101a-3 and the axis of the needle body 101a-1 as an example, the connecting hole 101a-3 is inclined along the direction from one side to the other side of the inner cavity of the connecting needle body 101a-1, toward the direction close to the needle tip 101a-2, to facilitate the entry of cleaning liquid and other liquids into the flow channel 102b-1.
  • the number of reagent needles 101a and cavities 102a is at least 3, among which at least one cavity 102a is used to store cleaning liquid, and can be directly connected to the fluid power component 104 through the reagent needle 101a, specifically, the reagent needle 101a and the fluid power component 104 are connected through the first pipeline 109a; at least one cavity 102a is used to store reagent, and can be directly connected to the fluid control component 105 through the reagent needle 101a, specifically, the reagent needle 101a and the fluid power component 104 are connected through the second pipeline 109b; at least one cavity 102a is used to store waste liquid, and can be directly connected to the fluid power component 104 through the reagent needle 101a, specifically, the reagent needle 101a and the fluid power component 104 are connected through the third pipeline 109c.
  • the communication between the reagent needle 101a and the fluid power part 104 refers to the state in which the reagent needle 101a is in contact with the cavity 102a
  • the flow cell 103 is used to store samples to be reacted. It should be noted that the samples to be reacted are set according to actual needs.
  • the fluid control component 105 switches to the flow pool 103 to communicate with the cavity 102a containing the reagent, and the fluid power component 104 drives the reagent into the flow pool 103 for reaction.
  • the fluid power component 104 When the reaction between the sample to be reacted and the corresponding reagent is completed, the fluid power component 104 is connected to the flow pool 103 and the cavity 102a for storing waste liquid, so that the waste liquid generated by the reaction is transported to the cavity 102a for storing waste liquid through the fluid power component 104.
  • the fluid power component 104 connects the flow pool 103 and the cavity 102a for storing the cleaning liquid, so that the cleaning liquid is driven into the flow pool 103 through the fluid power component 104, and the cleaning liquid is driven through the reagent needle 101a to the corresponding cavity 102a to complete reverse cleaning.
  • each cleaning solution needs to clean all the pipelines and reagent needles 101a corresponding to the reagents in sequence.
  • the reagent needle 101a When it is necessary to empty the reagent needle 101a after reverse cleaning, the reagent needle 101a is not in contact with the cavity 102a, and the liquid in the reagent needle 101a is driven to the flow pool 103 through the fluid power part 104 to empty the liquid in the reagent needle 101a.
  • the cleaning liquid and the waste liquid are integrated in the cavity structure 102 , realizing an integrated design of the reagent, the cleaning liquid and the waste liquid, reducing the number of parts and improving the integration of the liquid path system 100 .
  • the fluid system 100 further includes a fluid reservoir module 107 .
  • the fluid reservoir module 107 is directly connected to the fluid power member 104 through a first pipeline 109 a for storing cleaning fluid.
  • the number of the reagent needles 101 a and the number of the cavities 102 a are at least 2, and each cavity 102 a can be directly connected to the fluid control component 105 through the reagent needle 101 a.
  • At least one cavity 102a is used to store reagents. Specifically, the cavity 102a is connected to the fluid control component through the reagent needle 101a and the second pipeline 109b. At least one cavity 102a is used to store waste liquid. Specifically, the cavity 102a is connected to the fluid control component through the reagent needle 101a and the third pipeline 109c. The flow cell 103 is used to store samples to be reacted.
  • the fluid control component 105 switches to the flow pool 103 to communicate with the cavity 102a containing the reagent, and the fluid power component 104 drives the reagent into the flow pool 103 for reaction.
  • the fluid control component 105 switches to the flow pool 103 to communicate with the cavity 102a for holding waste liquid, and the fluid power component 104 transports the waste liquid generated by the reaction to the cavity 102a for holding waste liquid.
  • the fluid control component 105 When cleaning the reagent needle 101a corresponding to the reagent participating in the reaction, the fluid control component 105 is switched to connect the fluid reservoir module 107, the flow pool 103 and the reagent needle 101a to be cleaned, so that the cleaning liquid in the fluid reservoir module 107 is driven through the flow pool 103 and the reagent needle 101a to the corresponding cavity 102a through the fluid power component 104 to complete reverse cleaning.
  • the reagent needle 101a When the reagent needle 101a after reverse cleaning needs to be emptied, the reagent needle 101a is placed in non-contact with the cavity 102a, and the liquid in the reagent needle 101a is driven to the flow pool 103 by the fluid power member 104 to empty the liquid in the reagent needle 101a.
  • a waste liquid container is integrated in the cavity structure 102 , thereby realizing an integrated design of reagents and waste liquids, reducing the number of parts, and improving the integration of the liquid circuit system 100 .
  • the fluid system 100 further includes a fluid reservoir module 107 .
  • the fluid reservoir module 107 is in communication with the fluid power member 104 via a first pipeline 109 a for storing cleaning fluid.
  • the number of reagent needles 101a and cavities 102a is at least 2, at least one cavity 102a is used to store reagents, and is directly connected to the fluid control component 105 through the reagent needle 101a, specifically, the reagent needle 101a and the fluid control component 105 are connected through the second pipeline 109b; at least one cavity 102a is used to store waste liquid, and is directly connected to the fluid power component 104 through the reagent needle 101a, specifically, the reagent needle 101a and the fluid power component 104 are connected through the third pipeline 109c.
  • the flow cell 103 is used to store samples to be reacted.
  • the fluid control component 105 switches to the flow pool 103 to communicate with the cavity 102a containing the reagent, and the fluid power component 104 drives the reagent into the flow pool 103 for reaction.
  • the fluid power component 104 is connected to the flow cell 103 and the cavity 102a for containing waste liquid, so as to directly transport the waste liquid generated by the reaction into the cavity 102a for containing waste liquid.
  • the fluid control component 105 When cleaning the reagent needle 101a corresponding to the reagent participating in the reaction, the fluid control component 105 is switched to connect the fluid reservoir module 107, the flow pool 103 and the reagent needle 101a to be cleaned, so that the cleaning liquid in the fluid reservoir module 107 is driven through the flow pool 103 and the reagent needle 101a to the corresponding cavity 102a through the fluid power component 104 to complete reverse cleaning.
  • the reagent needle 101a When the reagent needle 101a after reverse cleaning needs to be emptied, the reagent needle 101a is placed in non-contact with the cavity 102a, and the liquid in the reagent needle 101a is driven to the flow pool 103 by the fluid power member 104 to empty the liquid in the reagent needle 101a.
  • This embodiment is similar to the second embodiment, in that a waste liquid container is integrated in the cavity structure 102 , thereby realizing an integrated design of reagents and waste liquids, reducing the number of parts, and improving the integration of the liquid path system 100 .
  • the fluid system 100 further includes a fluid reservoir module 107 .
  • the fluid reservoir module 107 is directly connected to the fluid power member 104 through a first pipeline 109 a for storing waste fluid.
  • the number of the reagent needles 101 a and the number of the cavities 102 a are at least 2, and each cavity 102 a can be directly connected to the fluid control component 105 through the reagent needle 101 a.
  • At least one cavity 102a is used to store reagents, and specifically, the reagent needle 101a is connected to the fluid control component 105 through a second pipeline 109b. At least one cavity 102a is used to store cleaning liquid, and specifically, the reagent needle 101a is connected to the fluid control component 105 through a third pipeline 109c.
  • the flow cell 103 is used to store samples to be reacted.
  • the fluid control component 105 switches to the flow pool 103 to communicate with the cavity 102a containing the reagent, and the fluid power component 104 drives the reagent into the flow pool 103 for reaction.
  • the fluid power component 104 is directly connected to the flow cell 103 and the fluid reservoir module 107 to transport the waste liquid generated by the reaction to the fluid reservoir module 107 .
  • the fluid control component 105 switches to the flow pool 103 to connect with the reagent needle 101a to be cleaned, and the fluid power component 104 drives the cleaning liquid into the flow pool 103.
  • the cleaning liquid flows through the reagent needle 101a and enters the corresponding cavity 102a to complete reverse cleaning.
  • the reagent needle 101a When the reagent needle 101a after reverse cleaning needs to be emptied, the reagent needle 101a is not in contact with the cavity 102a, and the liquid in the reagent needle 101a is driven to the flow cell 103 and/or the fluid reservoir module 107 by the fluid power member 104 to empty the liquid in the reagent needle 101a.
  • the cleaning liquid is integrated into the cavity structure 102 , realizing an integrated design of the reagent and the cleaning liquid, reducing the number of parts and improving the integration of the liquid circuit system 100 .
  • the fluid system 100 further includes a fluid reservoir module 107 .
  • the fluid reservoir module 107 is directly connected to the fluid power member 104 through a first pipeline 109 a for storing waste fluid.
  • the number of reagent needles 101a and cavities 102a is at least 2. At least one cavity 102a is used to store reagents, and can be directly connected to the fluid control component 105 through the reagent needle 101a. Specifically, the cavity 102a and the fluid control component 105 are connected through the reagent needle 101a and the second pipeline 109b; at least one cavity 102a is used to store cleaning liquid, and can be directly connected to the fluid power component 104 through the reagent needle 101a. Specifically, the cavity 102a and the fluid power component 104 are connected through the reagent needle 101a and the third pipeline 109c.
  • the flow cell 103 is used to store samples to be reacted.
  • the fluid control component 105 switches to the flow pool 103 to communicate with the cavity 102a containing the reagent, and the fluid power component 104 drives the reagent into the flow pool 103 for reaction;
  • the fluid power component 104 is directly connected to the flow cell 103 and the fluid reservoir module 107 to transport the waste liquid generated by the reaction to the fluid reservoir module 107;
  • the fluid power part 104 connects the flow pool 103 and the cavity 102a containing the cleaning liquid to drive the cleaning liquid into the flow pool 103, and the cleaning liquid flows through the reagent needle 101a into the corresponding cavity 102a to complete reverse cleaning;
  • the reagent needle 101a When the reagent needle 101a after reverse cleaning needs to be emptied, the reagent needle 101a is not in contact with the cavity 102a, and the liquid in the reagent needle 101a is driven to the flow cell 103 and/or the fluid reservoir module 107 by the fluid power member 104 to empty the liquid in the reagent needle 101a.
  • the cleaning fluid is integrated into the cavity structure 102, realizing an integrated design of the reagents and the cleaning fluid, reducing the number of parts and improving the integration of the liquid circuit system 100.
  • the fluid reservoir module 107 includes a waste liquid reservoir module 107a and a cleaning liquid reservoir module 107b which are respectively connected to the fluid power component 104.
  • the waste liquid reservoir module 107a is connected to the fluid power component 104 through a first pipeline 109a
  • the cleaning liquid reservoir module 107b is connected to the fluid power component 104 through a second pipeline 109b.
  • the waste liquid reservoir module 107a is used to hold waste liquid
  • the cleaning liquid reservoir module 107b is used to hold cleaning liquid
  • the flow cell 103 is used to store samples to be reacted.
  • the number of reagent needles 101a and cavities 102a is at least one. At least one cavity 102a is used to store reagents and is directly connected to the fluid control component 105 through the reagent needle 101a. Specifically, the reagent needle 101a is connected to the fluid control component 105 through the third pipeline 109c.
  • the fluid control component 105 switches to the flow pool 103 to communicate with the cavity 102a containing the reagent, and the fluid power component 104 drives the reagent into the flow pool 103 for reaction.
  • the fluid power component 104 is connected to the flow cell 103 and the waste liquid reservoir module 107a to directly transport the waste liquid generated by the reaction to the waste liquid reservoir module 107a.
  • the fluid power component 104 When cleaning the reagent needle 101a corresponding to the reagent participating in the reaction, the fluid power component 104 connects the flow pool 103 and the cleaning liquid reservoir module 107b, so that the cleaning liquid in the fluid reservoir module 107 is driven by the fluid power component 104 to flow through the flow pool 103 and the reagent needle 101a in sequence to the corresponding cavity 102a to complete reverse cleaning.
  • the reagent needle 101a When the reagent needle 101a after reverse cleaning needs to be emptied, the reagent needle 101a is placed in non-contact with the cavity 102a, and the liquid in the reagent needle 101a is driven to the flow pool 103 by the fluid power member 104 to empty the liquid in the reagent needle 101a.
  • a large amount of cleaning liquid can be prepared to support off-machine cleaning of multiple detection tasks.
  • the present application provides a gene sequencer, including a sequencer body and a liquid path system 100 as in any one of the above embodiments, wherein the liquid path system 100 is installed on the sequencer body.
  • the gene sequencer provided in the present application includes the liquid path system 100 in any one of the above-mentioned embodiments, the above-mentioned liquid path system 100 is included in the gene sequencer disclosed in the present application.
  • the present application provides a blood cell meter, including a blood cell meter body and a liquid path system 100 as in any one of the above embodiments, wherein the liquid path system 100 is installed on the blood cell meter body.
  • the blood cell analyzer provided in the present application includes the fluid path system 100 in any one of the above-mentioned embodiments, the above-mentioned fluid path systems 100 are all included in the blood cell analyzer disclosed in the present application.
  • the present application provides a chemiluminescent immunoassay analyzer, comprising an analyzer body and a fluid path system 100 as in any one of the above embodiments, wherein the fluid path system 100 is installed on the analyzer body.
  • the chemiluminescent immunoassay analyzer provided in the present application includes the fluid path system 100 in any one of the above embodiments, the above disclosed fluid path system 100 is included in the chemiluminescent immunoassay analyzer disclosed in the present application.
  • the present application provides a flow cytometer, including a cytometer body and a fluid path system 100 as in any one of the above embodiments, wherein the fluid path system 100 is installed on the cytometer body.
  • the flow cytometer provided in the present application includes the fluid path system 100 in any one of the above embodiments, the fluid path system 100 disclosed above is included in the flow cytometer disclosed in the present application.
  • the present application provides a detection device, including a device body and a liquid path system 100 as in any one of the above embodiments, wherein the liquid path system 100 is installed on the device body.
  • the detection device provided in the present application includes the fluid path system 100 in any one of the above embodiments, the fluid path system 100 disclosed above is included in the detection device disclosed in the present application.
  • the present application provides a sorting method, comprising:
  • Step S1 providing a fluid path system 100 .
  • the fluid path system 100 is the fluid path system 100 in any one of the above embodiments.
  • Step S2 injecting the sample to be reacted into the flow cell 103 and injecting the reagent into the cavity structure 102 .
  • the cleaning liquid can be connected externally separately, or the cleaning liquid can be stored in advance in the cavity structure 102 of the liquid path system 100 .
  • Step S3 contact the reagent needle assembly 101 with the cavity structure 102, and start the fluid power component 104 to drive the reagent into the flow cell 103 to react with the sample to be reacted.
  • the fluid power component 104 is a power component such as a syringe pump, a plunger pump, a diaphragm pump, a piezoelectric pump or a gear pump.
  • Step S3 The waste liquid generated by the reaction is positively pumped out from the flow cell 103 through the fluid power component 104 .
  • Step S4 The cleaning liquid is delivered to the reagent needle 101a and the cavity structure 102 through the fluid power component 104 to reversely clean the reagent needle 101a.
  • Step S5 The reagent needle assembly 101 is placed in a non-contact cavity structure 102 , and the fluid in the reagent needle 101 a is driven into the flow cell 103 through the fluid power element 104 .
  • system used in this application is a method for distinguishing different components, elements, parts, parts or assemblies at different levels.
  • the word can be replaced by other expressions.
  • first and second are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as “first” and “second” may explicitly or implicitly include one or more of the features.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente demande concerne un système de circulation de liquide et un dispositif associé, ainsi qu'un procédé de nettoyage. Le système de circulation de liquide comprend un ensemble aiguille de réactif ; une structure de cavité pouvant se déplacer par rapport à l'ensemble aiguille de réactif de manière à être en contact ou non avec celui-ci ; une cuve à circulation dont une extrémité est en communication directe avec la cavité interne de l'ensemble aiguille de réactif ou en communication avec la cavité interne de l'ensemble aiguille de réactif au moyen d'une canalisation ; et un élément d'alimentation en liquide en communication directe avec l'autre extrémité de la cuve à circulation ou en communication avec l'autre extrémité de la cuve à circulation au moyen d'une canalisation, l'élément d'alimentation en liquide étant utilisé pour acheminer un liquide dans la cuve à circulation vers la structure de la cavité ou pour acheminer un liquide dans la structure de la cavité vers la cuve à circulation, et pour pomper un liquide dans une aiguille de réactif dans la cuve à circulation lorsque l'ensemble aiguille de réactif n'est pas en contact avec la structure de la cavité. Selon la présente demande, l'ensemble du système de circulation de liquide peut être nettoyé efficacement et en profondeur.
PCT/CN2022/140259 2022-12-20 2022-12-20 Système de circulation de liquide et son dispositif associé, et procédé de nettoyage WO2024130534A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2022/140259 WO2024130534A1 (fr) 2022-12-20 2022-12-20 Système de circulation de liquide et son dispositif associé, et procédé de nettoyage

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2022/140259 WO2024130534A1 (fr) 2022-12-20 2022-12-20 Système de circulation de liquide et son dispositif associé, et procédé de nettoyage

Publications (1)

Publication Number Publication Date
WO2024130534A1 true WO2024130534A1 (fr) 2024-06-27

Family

ID=91587260

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2022/140259 WO2024130534A1 (fr) 2022-12-20 2022-12-20 Système de circulation de liquide et son dispositif associé, et procédé de nettoyage

Country Status (1)

Country Link
WO (1) WO2024130534A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102262164A (zh) * 2011-07-20 2011-11-30 长春迪瑞医疗科技股份有限公司 全自动便潜血分析仪的液路系统
CN109370890A (zh) * 2018-10-25 2019-02-22 广州市金圻睿生物科技有限责任公司 基因测序仪、液路系统及其自动检测方法
US20210069707A1 (en) * 2017-12-15 2021-03-11 Creoptix Ag Assemblies and methods for screening sample fluids
CN114994223A (zh) * 2022-04-29 2022-09-02 天津博纳艾杰尔科技有限公司 一种自动进样液路系统及自动进样方法
CN115264395A (zh) * 2022-08-31 2022-11-01 深圳太古语科技有限公司 一种流体系统
CN115367255A (zh) * 2021-05-19 2022-11-22 深圳华大智造科技股份有限公司 试剂盒

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102262164A (zh) * 2011-07-20 2011-11-30 长春迪瑞医疗科技股份有限公司 全自动便潜血分析仪的液路系统
US20210069707A1 (en) * 2017-12-15 2021-03-11 Creoptix Ag Assemblies and methods for screening sample fluids
CN109370890A (zh) * 2018-10-25 2019-02-22 广州市金圻睿生物科技有限责任公司 基因测序仪、液路系统及其自动检测方法
CN115367255A (zh) * 2021-05-19 2022-11-22 深圳华大智造科技股份有限公司 试剂盒
CN114994223A (zh) * 2022-04-29 2022-09-02 天津博纳艾杰尔科技有限公司 一种自动进样液路系统及自动进样方法
CN115264395A (zh) * 2022-08-31 2022-11-01 深圳太古语科技有限公司 一种流体系统

Similar Documents

Publication Publication Date Title
JP5172716B2 (ja) 試薬カートリッジ
JP5980030B2 (ja) 生化学処理装置
TWI641823B (zh) 流體整合模組及其適用之多流體系統之流體運作的調控方法
CN109569754B (zh) 单指标微流控芯片及其生产方法、使用方法
CN110785649A (zh) 样本过滤装置
CN215711746U (zh) 试剂加注装置
CN101178413A (zh) 液滴喷出头、检查装置和检查装置的使用方法
WO2024130534A1 (fr) Système de circulation de liquide et son dispositif associé, et procédé de nettoyage
CN114127247B (zh) 流体运输系统、方法及应用该系统、方法的流体使用装置
CN108339578A (zh) 液滴进样器以及使用其的液滴进样方法
US11426721B2 (en) Bubble eliminating structure, bubble eliminating method, and agitating method using the same
US20200164372A1 (en) Analysis system for testing a sample
EP2466316B1 (fr) Systèmes fluidiques, conteneur de fluide et procédés de lavage de conduits de fluide
CN113695322B (zh) 一种采样针清洗装置及其清洗方法
CN101865929B (zh) 自动试样分析器及其部件
US20230184800A1 (en) Reagent reservoirs and related systems and methods
CN220231771U (zh) 样本分析系统
CN115676759A (zh) 试剂加注装置和方法
JP2009183716A (ja) アクセス孔を有する透析装置
CN112705503B (zh) 清洗组件、引入装置与流体系统
CN1912617B (zh) 自动试样分析器及其部件
CN221148709U (zh) 液体输送装置及样本分析仪
US20190240661A1 (en) Cartridge and analysis system for testing a sample
CN118731400A (zh) 一种样本分析仪、样本检测方法
US20240299926A1 (en) Metering head and fluidic system for receiving and metering at least one medium

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22968837

Country of ref document: EP

Kind code of ref document: A1