WO2024127603A1 - 植物エキスの製造方法 - Google Patents

植物エキスの製造方法 Download PDF

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Publication number
WO2024127603A1
WO2024127603A1 PCT/JP2022/046283 JP2022046283W WO2024127603A1 WO 2024127603 A1 WO2024127603 A1 WO 2024127603A1 JP 2022046283 W JP2022046283 W JP 2022046283W WO 2024127603 A1 WO2024127603 A1 WO 2024127603A1
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Prior art keywords
plant extract
tobacco
producing
filtration
less
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PCT/JP2022/046283
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English (en)
French (fr)
Japanese (ja)
Inventor
裕介 七崎
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Japan Tobacco Inc
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Japan Tobacco Inc
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Publication date
Application filed by Japan Tobacco Inc filed Critical Japan Tobacco Inc
Priority to PCT/JP2022/046283 priority Critical patent/WO2024127603A1/ja
Priority to JP2024564083A priority patent/JPWO2024127603A1/ja
Priority to KR1020257021886A priority patent/KR20250116711A/ko
Priority to EP22968513.6A priority patent/EP4635596A1/en
Priority to CN202280102486.2A priority patent/CN120529955A/zh
Publication of WO2024127603A1 publication Critical patent/WO2024127603A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B13/00—Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • A24B15/12—Chemical features of tobacco products or tobacco substitutes of reconstituted tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • A24B15/16—Chemical features of tobacco products or tobacco substitutes of tobacco substitutes
    • A24B15/167—Chemical features of tobacco products or tobacco substitutes of tobacco substitutes in liquid or vaporisable form, e.g. liquid compositions for electronic cigarettes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/241—Extraction of specific substances
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24C—MACHINES FOR MAKING CIGARS OR CIGARETTES
    • A24C5/00—Making cigarettes; Making tipping materials for, or attaching filters or mouthpieces to, cigars or cigarettes
    • A24C5/47—Attaching filters or mouthpieces to cigars or cigarettes, e.g. inserting filters into cigarettes or their mouthpieces
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24D—CIGARS; CIGARETTES; TOBACCO SMOKE FILTERS; MOUTHPIECES OF CIGARS OR CIGARETTES; MANUFACTURE OF TOBACCO SMOKE FILTERS OR MOUTHPIECES
    • A24D1/00—Cigars; Cigarettes
    • A24D1/20—Cigarettes specially adapted for simulated smoking devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/42—Electrodialysis; Electro-osmosis ; Electro-ultrafiltration; Membrane capacitive deionization
    • B01D61/44—Ion-selective electrodialysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/58—Multistep processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0211—Solvent extraction of solids in combination with an electric or magnetic field
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0288—Applications, solvents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/02—Details relating to pores or porosity of the membranes
    • B01D2325/0283—Pore size

Definitions

  • the present invention relates to a method for producing a plant extract.
  • the plant extract obtained by extracting a plant may contain not only the target component but also undesirable components, and it is important to separate and remove such undesirable specific components.
  • the plant extract obtained by extracting tobacco plants contains important components as luxury items, such as aroma components that have a positive effect on the aroma.
  • Non-Patent Document 1 a precipitation membrane separation method using pH adjustment
  • Patent Document 3 a filtration method using activated carbon
  • Patent Document 4 a distillation method
  • Examples of methods for removing unnecessary components such as proteins include a method using enzymatic decomposition (Patent Document 5), and so-called ultrafiltration and microfiltration methods that can filter by molecular weight (Patent Documents 6 to 8).
  • methods for separating nitrate nitrogen include ultrafiltration or reverse osmosis membrane separation (Patent Document 9), and electrodialysis (Patent Document 10).
  • electrodialysis is a useful technique because it can also be used to remove TSNA from tobacco plants (Patent Documents 11 and 12).
  • the precipitation membrane separation method using pH adjustment requires the use of alkali, which requires the laborious operation of returning the pH to acidic.
  • the distillation method may decompose desirable components, and when filtration is used, the laborious regeneration and maintenance of the membrane is required.
  • the separation ability of the electrodialysis method may decrease if impurities are present in the extract.
  • the present invention aims to provide a method for producing a plant extract in which proteins and other impurities are efficiently separated and removed from a plant extract by a salting-out process to aggregate the proteins and maximize their size, making it possible to separate and remove the proteins even through large pores through which proteins cannot normally be filtered, and unnecessary salts after separation and removal are removed by electrodialysis to obtain a plant extract.
  • the electrical conductivity of the plant extract increases by 10 to 150 mS/cm compared to before and after the addition of the inorganic salt.
  • a method for producing a plant extract [2] The method for producing the plant extract according to [1], further comprising a step of removing the added inorganic salt by subjecting the filtrate to electrodialysis.
  • the inorganic salt is at least one selected from the group consisting of sodium chloride, potassium chloride, magnesium chloride, calcium chloride, sodium sulfate, potassium sulfate, magnesium sulfate, calcium sulfate, copper sulfate, ferrous sulfate, zinc sulfate, sodium carbonate, potassium carbonate, calcium carbonate, sodium bicarbonate, potassium hydroxide, calcium hydroxide, sodium nitrite, tripotassium phosphate, tricalcium phosphate, dipotassium hydrogen phosphate, and disodium hydrogen phosphate.
  • the inorganic salt is at least one selected from the group consisting of sodium chloride, potassium chloride, magnesium chloride, calcium chloride, sodium sulfate, potassium sulfate, magnesium sulfate, calcium sulfate, copper sulfate, ferrous sulfate, zinc sulfate, sodium carbonate, potassium carbonate, calcium carbonate, sodium bicarbonate, potassium hydroxide
  • [7] The method for producing a plant extract according to either [5] or [6], wherein the filtration in the filtration step is microfiltration and the average pore size of the filtration membrane is 3.0 ⁇ m or less.
  • a method for producing a tobacco product having a tobacco rod wrapped in cigarette paper around which a tobacco filler is wrapped, and a mouthpiece portion comprising the step of adding a plant extract produced by the method for producing a plant extract according to any one of [1] to [10] to at least one selected from the group consisting of the tobacco filler and the cigarette paper.
  • a method for producing an oral pouch product having an oral composition and a pouch for packaging the oral composition comprising: A method for producing an oral pouch product, comprising the step of adding a plant extract produced by the method for producing a plant extract according to any one of [1] to [10] to the oral composition.
  • the present invention provides a method for producing a plant extract by efficiently separating and removing impurities such as proteins from a plant extract, thereby making it possible to control the amount of alkaloids. Furthermore, it provides a method for producing a tobacco product or an oral pouch product using this plant extract.
  • 1 is a schematic diagram of a tobacco product according to an embodiment of the present invention.
  • 1 is a schematic diagram of an electrically heated tobacco product according to an embodiment of the present invention.
  • 1 is a schematic diagram of an electrically heated tobacco product according to an embodiment of the present invention.
  • 1 is a graph showing the results of nitrate ion analysis after electrodialysis in an example.
  • the method for producing a plant extract according to an embodiment of the present invention includes an inorganic salt adding step of adding an inorganic salt to a plant extract, and a filtration step of filtering the plant extract by ultrafiltration or microfiltration to obtain a filtrate.
  • the inorganic salt addition step the electrical conductivity of the plant extract increases by 10 to 150 mS/cm compared to before and after the addition of the inorganic salt.
  • the method for producing a plant extract according to this embodiment may include steps other than the inorganic salt addition step and the filtration step.
  • the method for producing a plant extract according to this embodiment may include an extraction step of extracting a plant dispersion containing a plant to obtain an extract.
  • the macromolecules such as proteins in the extract obtained in the extraction step are agglomerated by salting out to increase their size, impurities larger than a specific size are separated and removed by cross-flow filtration, and then specific substances are separated by electrodialysis to obtain a plant extract from which specific impurities have been removed.
  • Impurities larger than a specific size include proteins normally contained in plants, and therefore the raw material used in the extraction step is not particularly limited as long as it is made from a plant.
  • the type of plant (extraction target) used in the extraction step is not particularly limited, and may be a seed plant or a non-seed plant. Seed plants may be either gymnosperms or angiosperms. Gymnosperms include cycads such as cycads, ginkgos such as ginkgo, pines such as red pine or black pine, and gnetaceae such as gnemon.
  • Angiosperms include Solanaceae such as tobacco or eggplant, Asteraceae such as chrysanthemum, Orchidaceae such as orchid, Fabaceae such as pea, Poaceae such as rice, Rubiaceae such as coffee, Lamiaceae such as mint, Euphorbiaceae such as spurge, and Cyperaceae such as sedge.
  • non-seed plants include ferns such as bracken or osmanthus, mosses such as liverwort or hornwort, or algae such as kelp or wakame.
  • the method for producing a plant extract according to this embodiment makes it easy to separate and remove unnecessary components, such as components that are the source of components of concern, such as nitrate nitrogen proteins, which are precursors of tobacco-specific nitrosamines (TSNAs), and components that do not directly affect the aroma, such as proteins contained in many plants, such as Rubisco (RuBisCO),
  • the plant used in this embodiment i.e., the plant to be extracted and contained in the plant extract
  • the part of the plant used is not particularly limited, and for example, in the case of a seed plant, any of the leaves, stems, or roots may be used. From the viewpoints of ease of extraction and ease of availability, however, leaves are preferred.
  • tobacco leaves hereinafter, also referred to as "tobacco leaves"
  • the type of tobacco leaf is not particularly limited, and examples thereof include flue-cured, burley, orient, native, other Nicotiana tabacum varieties, Nicotiana rustica varieties, etc. These varieties can be used alone, but can also be blended in the process of turning tobacco leaves into processed tobacco leaves to obtain the desired flavor.
  • the plant dispersion liquid is not particularly limited as long as it is a liquid containing a plant, and the solvent may be any solvent that can dissolve the substance to be extracted, and may be an organic solvent or an inorganic solvent.
  • the solvent is preferably one that can dissolve nicotine, and examples of the solvent include water, ethylene glycol, propylene glycol, and ethanol, but water is preferable from the viewpoints of safety, cost, and availability. These substances may be contained in the plant extract finally obtained.
  • the plant content in the plant dispersion is not particularly limited, and is usually 5% by weight or more, preferably 6% by weight or more, more preferably 7.5% by weight or more, even more preferably 8% by weight or more, and particularly preferably 10% by weight or more, and is usually 25% by weight or less, preferably 20% by weight or less, more preferably 15% by weight or less, and particularly preferably 13% by weight or less.
  • the solvent content in the plant dispersion is not particularly limited, and is usually 75% by weight or more, preferably 77.5% by weight or more, more preferably 80% by weight or more, even more preferably 82.5% by weight or more, and particularly preferably 85% by weight or more, and is usually 99% by weight or less, preferably 97.5% by weight or less, more preferably 95% by weight or less, even more preferably 92.5% by weight or less, and particularly preferably 90% by weight or less.
  • the plant dispersion may contain components other than the plant and the solvent (other components), such as reducing sugars, natural sweeteners, artificial sweeteners, pH adjusters, buffers, preservatives, or flavorings.
  • components other than the plant and the solvent such as reducing sugars, natural sweeteners, artificial sweeteners, pH adjusters, buffers, preservatives, or flavorings.
  • the extraction method is not particularly limited, and any known method can be used, including a process in which the above-mentioned plant dispersion is heated to obtain an extract.
  • the heating temperature is not particularly limited, and is not limited as long as it is a temperature at which the plant dispersion liquid can be evaporated, and may be, for example, 50° C. or higher, preferably 55° C. or higher, more preferably 60° C. or higher, and even more preferably 70° C. or higher, and may be 100° C. or lower, preferably 95° C. or lower, more preferably 90° C. or lower, and even more preferably 88° C. or lower.
  • This heating temperature is particularly advantageous when using tobacco plants, i.e., when extracting nicotine.
  • the heating time is not particularly limited, and may be, for example, 5 minutes or more, preferably 10 minutes or more, more preferably 15 hours or more, even more preferably 30 hours or more, and may be 6 hours or less, even more preferably 3 hours or less.
  • This heating temperature is particularly advantageous when using tobacco plants, that is, when extracting nicotine.
  • the method for producing a plant extract according to the present embodiment includes an inorganic salt addition step of adding an inorganic salt to a plant extract.
  • the method for adding the inorganic salt to the plant extract is not particularly limited, and the inorganic salt can be added by any method.
  • the type of inorganic salt is not particularly limited, but is preferably at least one selected from the group consisting of sodium chloride, potassium chloride, magnesium chloride, calcium chloride, sodium sulfate, potassium sulfate, magnesium sulfate, calcium sulfate, copper sulfate, ferrous sulfate, zinc sulfate, sodium carbonate, potassium carbonate, calcium carbonate, sodium hydrogen carbonate, potassium hydroxide, calcium hydroxide, sodium nitrite, tripotassium phosphate, tricalcium phosphate, dipotassium hydrogen phosphate, and disodium hydrogen phosphate.
  • These inorganic salts may be used alone or in any combination and ratio of two or more.
  • the amount of inorganic salt added to the plant extract is not particularly limited, but the amount added relative to 100 parts by weight of the plant extract is preferably 2.04 parts by weight or more and 33.3 parts by weight or less, more preferably 3.09 parts by weight or more and 20 parts by weight or less, even more preferably 4.17 parts by weight or more and 18.5 parts by weight or less, and particularly preferably 5.00 parts by weight or more and 17.64 parts by weight or less.
  • the inorganic salt addition step inorganic salt is added, and the electrical conductivity of the plant extract is increased by the addition of inorganic salt.
  • the electrical conductivity increases, the amount of protein dispersible or soluble in water is limited, and aggregation of polymers such as proteins is promoted.
  • the degree of increase in electrical conductivity may be an increase of 10 to 150 mS/cm, preferably an increase of 20 to 130 mS/cm, more preferably an increase of 30 to 120 mS/cm, even more preferably an increase of 40 to 110 mS/cm, and particularly preferably an increase of 50 to 100 mS/cm.
  • the electrical conductivity can be measured using a compact electrical conductivity meter (LAQUAtwin manufactured by HORIBA).
  • the method for producing a plant extract according to this embodiment includes a filtration step of filtering the extract obtained in the above-mentioned extraction step and salting out step by cross-flow filtration to obtain a filtrate.
  • Cross-flow filtration is a filtration method in which the flow direction of the supply liquid and the filtration direction are different, for example, these directions are perpendicular (in this specification, "perpendicular” includes approximately perpendicular). Filtration by this method is suitable for concentrating large amounts of filtration, and is advantageous in that the difference between the flow direction of the supply liquid and the filtration direction makes it easy to prevent particle deposition and clogging on the filtration membrane surface, and also has a time-saving effect.
  • the method of cross-flow filtration is not particularly limited, and any known method can be used.
  • Cross-flow filtration can be performed using a cross-flow filter (for example, Lab Stak M2O manufactured by Alfa Laval).
  • the form of the filtration membrane in cross-flow filtration is not particularly limited, and may be planar or cylindrical, but is preferably cylindrical from the viewpoint of downsizing of the filter and efficient filtration.
  • the diameter can be 5 cm or more and 100 cm or less
  • the axial length can be 2 cm or more and 2000 cm or less.
  • the form in which the supply liquid (extraction liquid) flows is not particularly limited, and may be a circulation type or may not be a circulation type, but is preferably a circulation type from the viewpoint of downsizing of the filter and efficient filtration.
  • the material of the filtration membrane is not particularly limited, but typically includes polyester, polypropylene, polysulfone, hydrophilic polysulfone, polyethersulfone, fluororesin, and the like.
  • the thickness of the filtration membrane is not particularly limited, but from the viewpoint of preventing clogging and improving filtration efficiency, it is usually 0.01 ⁇ m or more, preferably 0.02 ⁇ m or more, more preferably 0.05 ⁇ m or more, even more preferably 0.07 ⁇ m or more, and particularly preferably 0.1 ⁇ m or more, and the upper limit may be, for example, 1 mm or less, 0.8 mm or less, 0.6 mm or less, 0.5 mm or less, or 0.3 mm or less.
  • the area of the filtration membrane can be appropriately set depending on the application.
  • the average pore size of the filtration membrane is not particularly limited, but from the viewpoint of preventing clogging and improving the efficiency of filtration, when using a microfiltration membrane, it is usually 0.1 ⁇ m or more, preferably 0.2 ⁇ m or more, more preferably 5.0 ⁇ m or less, and even more preferably 3.0 ⁇ m or less.
  • the pore size of the filtration membrane is usually 1,000 Da or more, preferably 10,000 Da or more, more preferably 50,000 Da or more, and even more preferably 20,000 Da or more, and also usually 5,000,000 Da or less, preferably 2,500,000 Da or less, more preferably 200,000 Da or less, and even more preferably 100,000 Da or less.
  • the average particle size of unnecessary components such as components that are the source of components of concern, such as nitrate nitrogen proteins, which are precursors of tobacco-specific nitrosamines (TSNAs), and components that do not directly affect the aroma, such as proteins contained in many plants, such as RuBisCO, is 50,000 Da (g/mol) or more and 80,000 Da or less, and the average particle size of nicotine is 162.23 Da, which allows efficient separation of components such as proteins and nicotine, and therefore the above range is advantageous particularly when using tobacco plants.
  • a multi-stage system using a plurality of membranes is also included.
  • the porosity of the membrane surface of the filtration membrane is not particularly limited.
  • the membrane surface flow rate relative to the average pore size of the filtration membrane is not particularly limited, but from the viewpoint of preventing clogging and improving the efficiency of filtration, it is usually 10 mL/min ⁇ cm2 or more, preferably 20 mL/min ⁇ cm2 or more, more preferably 30 mL/min ⁇ cm2 or more, and even more preferably 45 mL/min ⁇ cm2 or more, and usually 100 mL/min ⁇ cm2 or less, preferably 80 mL/min ⁇ cm2 or less, more preferably 70 mL/min ⁇ cm2 or less, even more preferably 60 mL/min ⁇ cm2 or less, and especially preferably 55 mL/min ⁇ cm2 or less.
  • the above range is advantageous, especially when using tobacco plants, from the viewpoint of the average particle size of the above unnecessary components contained in tobacco plants.
  • the temperature of the extract being supplied in cross-flow filtration is not particularly limited, but from the viewpoint of preventing clogging and improving filtration efficiency, it is usually 5°C or higher, preferably 10°C or higher, more preferably 20°C or higher, and particularly preferably 40°C or higher, and is usually 80°C or lower, preferably 70°C or lower, more preferably 60°C or lower, and particularly preferably 50°C or lower.
  • the above ranges are advantageous from the viewpoint of the viscosity of the tobacco plant, particularly when tobacco plants are used.
  • the content of inorganic salts in the filtrate is not particularly limited, but is preferably 10% by weight or less, more preferably 5% by weight or less, preferably 3% by weight or less, preferably 1% by weight or less, and may be 0% by weight (below the detection limit).
  • the lower limit of the content is not particularly limited, and may be more than 0% by weight, may be 0.5% by weight or more, may be 1% by weight or more, or may be 2% by weight or more.
  • the method for producing a plant extract according to this embodiment preferably includes a dialysis step of removing the added inorganic salts by electrodialysis of the filtrate obtained in the above filtration step.
  • electrodialysis a device equipped with an ion exchange membrane and electrodes that allow only ions to pass through is used, and by applying a voltage between the electrodes, ions in a solution can be made to pass through the ion exchange membrane, thereby removing certain substances such as inorganic salts.
  • the method of electrodialysis is not particularly limited, and any known method can be used, and the electrodialysis can be performed using an electrodialysis device (for example, MICRO ACILYZER S3 or ACILYZER EX3B manufactured by Astom Corporation).
  • the form of the filtration membrane in electrodialysis is not particularly limited, but it is usually a planar shape, and for example, filtration membranes of 10 to 300 cm x 5 to 100 cm can be arranged in multiple layers.
  • the form in which multiple filtration membranes are arranged is not particularly limited, and gaps may or may not be provided, but it is preferable to provide gaps.
  • the form in which the supply liquid (extract liquid) flows is not particularly limited, and it may or may not be a circulating type, but from the viewpoint of downsizing the filter and improving the efficiency of filtration, a circulating type is preferable.
  • the type and size of the electrodes used in the electrodialysis can be appropriately determined depending on the filtrate used and the apparatus for carrying out the electrodialysis.
  • the voltage applied between the electrodes is not particularly limited, but from the viewpoint of efficient electrodialysis, it is usually 1.00 V or more, preferably 2.00 V or more, more preferably 3.00 V or more, even more preferably 4.00 V or more, particularly preferably 5.00 V or more, may be 10.00 V or more, may be 15.00 V or more, and is usually 60.00 V or less, preferably 50.00 V or less, more preferably 40.00 V or less, even more preferably 35.00 V or less, and particularly preferably 30.00 V or less.
  • the voltage is too high, not only separation and decomposition of water (H + and OH - ) occurs, but also separation and decomposition of other components may occur, so it is preferably 30.00 V or less.
  • the concentration of each component contained in the plant extract can be controlled by controlling the voltage.
  • the nicotine content in the plant extract can be changed by adjusting the voltage. As will be seen from the examples described below, at 30.00 V the amount of nicotine can be efficiently reduced, and at 10.00 V the amount of nicotine can be efficiently maintained.
  • the material of the filtration membrane is not particularly limited, but typically includes regenerated cellulose, cellulose ester, polyacrylonitrile, polysulfone, polymethyl methacrylate, ethylene-vinyl alcohol copolymer, and the like.
  • the thickness of the filtration membrane is not particularly limited, but from the viewpoint of preventing clogging and improving filtration efficiency, it is usually 0.05 ⁇ m or more, preferably 0.1 ⁇ m or more, more preferably 0.15 ⁇ m or more, even more preferably 0.17 ⁇ m or more, and particularly preferably 0.2 ⁇ m or more, and is usually 1 ⁇ m or less, preferably 0.8 ⁇ m or less, more preferably 0.5 ⁇ m or less, even more preferably 0.4 ⁇ m or less, and particularly preferably 0.3 ⁇ m or less.
  • the area of the filtration membrane can be appropriately set depending on the application.
  • the average pore size of the filtration membrane is not particularly limited, but from the viewpoint of preventing clogging and improving the efficiency of filtration, when using an ultrafiltration membrane, it is usually 1,000 Da or more, preferably 1,500 Da or more, more preferably 2,000 Da or more, and even more preferably 2,500 Da ⁇ m or more, and also usually 100,000 Da or less, and even more preferably 50,000 Da or less.
  • the average particle size of unnecessary components such as components that are the source of worrying components such as nitrate nitrogen proteins, which are precursors of tobacco-specific nitrosamines (TSNA), and components that do not directly affect aroma such as proteins contained in many plants such as Rubisco (RuBisCO)
  • TSNA tobacco-specific nitrosamines
  • RuBisCO tobacco-specific nitrosamines
  • the average particle size of nicotine is 162.3 g/mol (Da), and since it can efficiently separate components such as proteins and nicotine, the above range is particularly advantageous when using tobacco plants.
  • the porosity of the membrane surface of the filtration membrane is
  • the membrane surface flow rate relative to the average pore size of the filtration membrane is not particularly limited, but from the viewpoint of preventing clogging and improving the efficiency of filtration, it is usually 0.001 mL/min ⁇ cm2 or more, preferably 0.002 mL/min ⁇ cm2 or more, more preferably 0.003 mL/min ⁇ cm2 or more, even more preferably 0.004 mL/min ⁇ cm2 or more , particularly preferably 0.005 mL/min ⁇ cm2 or more, and is usually 40 mL/ min ⁇ cm2 or less, preferably 35 mL/min ⁇ cm2 or less, more preferably 25 mL/min ⁇ cm2 or less , and particularly preferably 20 mL/min ⁇ cm2 or less.
  • the above range is advantageous from the viewpoint of the average particle size of the above unnecessary components contained in the tobacco plant, especially when using a tobacco plant.
  • the temperature of the extract being fed in electrodialysis is not particularly limited and may be, for example, 10
  • the content of inorganic salts in the filtrate (essentially the plant extract) obtained in the filtration process is not particularly limited, but is preferably 10% by weight or less, more preferably 5% by weight or less, preferably 3% by weight or less, preferably 1% by weight or less, and may be 0% by weight (below the detection limit).
  • the lower limit of the content is not particularly limited, and may be more than 0% by weight, may be 0.5% by weight or more, may be 1% by weight or more, or may be 2% by weight or more.
  • the method for producing a plant extract according to the present embodiment may include a preparation step of preparing a plant dispersion liquid before the above-mentioned extraction step.
  • the method for preparing the plant dispersion liquid is not particularly limited, and may include, for example, a method in which raw materials that may be contained in the above-mentioned plant dispersion liquid other than the solvent are added to the above-mentioned solvent and stirred to be homogenous.
  • the method for producing a plant extract according to the present embodiment may include a concentrating step of concentrating the plant extract obtained by the dialysis step.
  • the method for concentrating is not particularly limited, and a known method can be applied, for example, it can be carried out using an apparatus such as an evaporator.
  • it is preferable to carry out the method by freeze concentration and the method for freeze concentration is not particularly limited, and a known method can be adopted.
  • Another embodiment of the present invention is a plant extract obtained by the above-mentioned manufacturing method.
  • the plant extract according to this embodiment is characterized by a low content of impurities of a specific size.
  • the plant extract obtained under the conditions of the above-mentioned manufacturing method is advantageous in reducing the concentration of unnecessary components such as components that are the source of components of concern, such as nitrate nitrogen proteins, which are precursors of tobacco-specific nitrosamines (TSNA), and components that do not directly affect aroma, such as proteins contained in many plants, such as RuBisCO, and is therefore preferred when using tobacco plants as the plant.
  • the content of components having a molecular weight of 50 Da (g/mol) or more and 50,000 Da or less in the tobacco extract is 15% by weight or less.
  • the viscosity of the plant extract at 25°C is not particularly limited, but from the viewpoint of ease of handling, it is usually 0.5 mPa ⁇ s or more, and more preferably 1.0 mPa ⁇ s or more, and is usually 15.0 mPa ⁇ s or less, and preferably 6.5 mPa ⁇ s or less, more preferably 6.0 mPa ⁇ s or less, and even more preferably 5.5 mPa ⁇ s or less.
  • the viscosity can be measured with a tuning fork vibratory viscometer (SV-A manufactured by AND Co., Ltd.).
  • the pH of the plant extract at 25°C is not particularly limited, but from the viewpoint of ease of use in tobacco products and oral pouch products, it is usually 7.5 or more, preferably 8.0 or more, more preferably 8.5 or more, and more preferably usually 9.0 or less.
  • the pH of the oral composition at the measurement temperature of 25° C. can be measured using a pH analyzer (e.g., LAQUA F-72 flat ISFET pH electrode manufactured by Horiba, Ltd.) by adding 20 ml of water to 2 g of the oral composition, shaking for 10 minutes, and measuring the supernatant.
  • a pH analyzer e.g., LAQUA F-72 flat ISFET pH electrode manufactured by Horiba, Ltd.
  • the calibration of the device is performed by three-point calibration using, for example, a phthalic acid pH standard solution (pH 4.01), a neutral phosphate pH standard solution (pH 6.86), and a borate pH standard solution (pH 9.18) (all from Wako Pure Chemical Industries, Ltd.).
  • a phthalic acid pH standard solution pH 4.01
  • a neutral phosphate pH standard solution pH 6.86
  • a borate pH standard solution pH 9.18
  • the uses of the plant extract obtained by the above-mentioned manufacturing method are not particularly limited, and it can be used in a wide range of fields depending on the concentrated components.
  • a plant extract with concentrated nicotine can be produced by the above-mentioned extraction, cross-flow filtration, and electrodialysis, and can be used, for example, in tobacco products and oral pouch products described below.
  • a tobacco product manufacturing method (hereinafter simply referred to as "a tobacco product manufacturing method"), is a tobacco product manufacturing method having a tobacco rod portion wrapped in cigarette paper that wraps a tobacco filler, and a mouthpiece portion, the tobacco product manufacturing method including a step of adding a plant extract produced by the above-mentioned plant extract manufacturing method to at least one selected from the group consisting of the tobacco filler and the cigarette paper.
  • the tobacco product according to the present embodiment can be produced by a known method, except for the step of adding the plant extract, for example, by wrapping the tobacco rod and the mouthpiece in tip paper.
  • the step of adding the plant extract is not particularly limited as long as it is carried out at any stage in the production of a tobacco product where the plant extract can be added to the tobacco filler and/or cigarette paper in the tobacco rod part.
  • the plant extract may be added to the tobacco filler and/or cigarette paper before wrapping the tobacco filler with cigarette paper, to the tobacco filler and/or cigarette paper after wrapping, after the tobacco rod part is produced, or after the tobacco rod part and the mouthpiece part are wrapped with tipping paper.
  • the method of adding the plant extract to the tobacco filler and/or cigarette paper is not particularly limited, and for example, the plant extract may be dropped onto the tobacco filler and/or cigarette paper, the plant extract may be applied to the tobacco filler and/or cigarette paper, or the tobacco filler and/or cigarette paper may be immersed in the plant extract.
  • the plant extract may also be added to the residue obtained when extracting the tobacco plant after drying, and this residue may be used as the tobacco filler.
  • this residue an extraction residue obtained when a tobacco plant is used as the plant in the extraction step in the production of the above-mentioned plant extract can be used, and from the viewpoint of such reusability, it is preferable to produce the plant extract using a tobacco plant.
  • the method of adding the plant extract is not particularly limited, and in addition to the above-mentioned dropping, application, and immersion, methods such as spraying and kneading can also be used.
  • the amount of plant extract added per 100 parts by weight of the tobacco rod is usually 0.5 parts by weight or more, preferably 1.0 parts by weight or more, more preferably 2.0 parts by weight or more, and even more preferably 5.0 parts by weight or more, and is usually 20 parts by weight or less, preferably 15 parts by weight or less, more preferably 12.5 parts by weight or less, and even more preferably 10 parts by weight or less.
  • the type of plant is not particularly limited; for example, a plant-derived flavor component can be added to the tobacco product, but it is preferable to use a tobacco plant, particularly from the viewpoint of being able to control the amount of nicotine.
  • the tobacco product manufactured by the tobacco product manufacturing method according to the present embodiment may be for cigarettes or for non-combustion heat-not-burn tobacco products. An example of a tobacco product will be described below, and each condition is particularly advantageous for a non-combustion heat-not-burn tobacco product.
  • the rod-shaped tobacco product 10 shown in Fig. 1 is a rod-shaped tobacco product including a tobacco rod portion 11, a mouthpiece portion 14, and a tipping paper 15 around which these are wound.
  • the mouthpiece portion may take any form, but Fig. 1 shows a form including a cooling segment 12 and a filter segment 13 including a filter medium, in which the cooling segment 12 is sandwiched adjacent to the tobacco rod portion 11 and the filter segment 13 in the axial direction (also referred to as the "long axis direction") of the tobacco product 10, and an opening V is provided concentrically in the circumferential direction of the cooling segment 12.
  • the opening V is usually a hole for promoting the inflow of air from the outside when the user inhales, and the inflow of this air can lower the temperature of the components and air flowing in from the tobacco rod portion 11.
  • components generated by heating include flavor components derived from flavorings, nicotine and tar derived from tobacco leaves, and aerosol components derived from the aerosol base material.
  • the aerosol base material is a base material for generating an aerosol.
  • the tobacco product 10 preferably has a cylindrical shape that satisfies an aspect ratio of 1 or greater, as defined below.
  • Aspect ratio h/w w is the width of the bottom surface of the columnar body (in this specification, the width of the bottom surface on the tobacco rod portion side), and h is the height, and it is preferable that h ⁇ w.
  • the long axis direction is defined as the direction indicated by h. Therefore, even if w ⁇ h, the direction indicated by h will be referred to as the long axis direction for convenience.
  • the shape of the bottom surface is not limited and may be a polygon, a rounded polygon, a circle, an ellipse, etc., and the width w is the diameter when the bottom surface is a circle, the major axis when the bottom surface is an ellipse, or the diameter of the circumscribing circle or the major axis of the circumscribing ellipse when the bottom surface is a polygon or a rounded polygon.
  • the length h of the tobacco product 10 in the longitudinal direction is not particularly limited, and is, for example, usually 40 mm or more, preferably 45 mm or more, and more preferably 50 mm or more, and is usually 100 mm or less, preferably 90 mm or less, and more preferably 80 mm or less.
  • the width w of the base of the column of the tobacco product 10 is not particularly limited, and is, for example, typically 5 mm or more, and preferably 5.5 mm or more, and typically 10 mm or less, and preferably 9 mm or less, and more preferably 8 mm or less.
  • the ratio of the lengths of the cooling segment and the filter segment in the longitudinal direction of the tobacco product is not particularly limited, but from the viewpoint of the delivery amount of flavoring, it is usually 0.60-1.40:0.60-1.40, 0.80-1.20:0.80-1.20, preferably 0.85-1.15:0.85-1.15, more preferably 0.90-1.10:0.90-1.10, and even more preferably 0.95-1.05:0.95-1.05.
  • the cooling effect By setting the ratio of the lengths of the cooling segment and the filter segment within the above range, the cooling effect, the effect of suppressing losses due to adhesion of the generated steam and aerosol to the inner wall of the cooling segment, and the function of adjusting the air volume and flavor of the filter are balanced, thereby realizing a good flavor and flavor intensity.
  • the cooling segment is made longer, the particulation of aerosols and the like is promoted and a good flavor can be realized, but if it is too long, the substances passing through will adhere to the inner wall.
  • the air resistance in the longitudinal direction per tobacco product 10 is not particularly limited, but from the standpoint of ease of smoking, it is usually 8 mmH2O or more, preferably 10 mmH2O or more, and more preferably 12 mmH2O or more, and is usually 100 mmH2O or less, preferably 80 mmH2O or less, and more preferably 60 mmH2O or less.
  • the airflow resistance is measured according to the ISO standard method (ISO6565:2015) using, for example, a filter airflow resistance meter manufactured by Cerulean Co., Ltd.
  • the airflow resistance refers to the air pressure difference between the first end face and the second end face when air is flowed at a predetermined air flow rate (17.5 cc/min) from one end face (first end face) to the other end face (second end face) in a state where air does not pass through the side faces of the tobacco product 10.
  • the unit is generally expressed in mmH 2 O. It is known that the relationship between the airflow resistance and the length of the tobacco product is proportional within the length range usually used (lengths of 5 mm to 200 mm), and if the length is doubled, the airflow resistance of the tobacco product doubles.
  • the form of the mouthpiece portion 14 is not particularly limited, and for example, as shown in FIG. 1 , it can be an embodiment in which it includes a cooling segment 12 and a filter segment 13 containing the above-mentioned filter material, and is configured so that the cooling segment 12 is sandwiched adjacent to the tobacco rod portion 11 and the filter segment 13 in the axial direction of the tobacco product 10.
  • the filter segment 13 is not particularly limited as long as it has a function as a general filter, and the form of a known filter segment used in tobacco products can be appropriately adopted.
  • a tow also simply called “tow”
  • Examples of the general functions of a filter include adjusting the amount of air mixed when inhaling aerosols, reducing flavor, and reducing nicotine and tar, but it is not necessary for the filter to have all of these functions.
  • electrically heated tobacco products which tend to produce fewer components and have a lower tobacco filler filling rate than cigarette products, one of the important functions is to suppress the filtering function while preventing the tobacco filler from falling.
  • the shape of the filter segment 13 can also be a known shape, for example, a cylindrical shape.
  • the manufacturing method of the filter segment 13 is not particularly limited, and a manufacturing method of a known filter segment can be appropriately adopted.
  • the form of the cooling segment 12 is not particularly limited, and any form of known cooling segments used in tobacco products may be appropriately adopted, for example, it may be a rod-shaped member sandwiched adjacent to the tobacco rod portion and the filter segment, and typically provided with a cavity such that the circumferential cross section of the cylinder or the like is hollow (hollow).
  • the manufacturing method of the cooling segment 12 is also not particularly limited, and any manufacturing method of known cooling segments may be appropriately adopted.
  • the cooling segment 12 may be provided with an opening V (also referred to as a "ventilation filter (Vf)" in this technical field) in the circumferential direction and concentrically, as shown in FIG. 1.
  • the form of the tobacco rod portion 11 is not particularly limited, and may be any form of a publicly known tobacco rod portion used in tobacco products, for example, a form in which a tobacco filler is wrapped in cigarette paper.
  • the manufacturing method of the tobacco rod portion 11 is also not particularly limited, and may be any method of manufacturing a publicly known tobacco rod portion.
  • the tobacco filler is not particularly limited, and known materials such as tobacco shreds and reconstituted tobacco sheets can be used.
  • the tobacco filler may also contain an aerosol base material.
  • the aerosol base material is a base material that generates an aerosol when heated, and examples of the aerosol base material include glycerin, propylene glycol, triacetin, 1,3-butanediol, and mixtures thereof.
  • the configuration of the cigarette paper is not particularly limited, and any known cigarette paper used in tobacco products can be appropriately adopted, for example, one containing pulp as a main component.
  • the manufacturing method of the cigarette paper is also not particularly limited, and any known manufacturing method of cigarette paper can be appropriately adopted.
  • the pulp may be produced from wood pulp such as softwood pulp or hardwood pulp, or may be produced by mixing with non-wood pulp that is generally used for cigarette paper for tobacco products, such as flax pulp, hemp pulp, sisal pulp, or esparto.
  • non-wood pulp that is generally used for cigarette paper for tobacco products, such as flax pulp, hemp pulp, sisal pulp, or esparto.
  • As the type of pulp chemical pulp produced by the kraft cooking method, acidic/neutral/alkaline sulfite cooking method, soda salt cooking method, etc., ground pulp, chemi-ground pulp, thermomechanical pulp, etc. can be used.
  • the configuration of the tipping paper 15 is not particularly limited, and any known tipping paper used in tobacco products can be appropriately adopted, for example, a tipping paper mainly composed of pulp.
  • the manufacturing method of the tipping paper 15 is also not particularly limited, and any known tipping paper manufacturing method can be appropriately adopted, and a commercially available product can also be used.
  • the pulp may be made from wood pulp such as softwood pulp or hardwood pulp, or may be made by mixing non-wood pulp that is generally used for cigarette paper for tobacco products, such as flax pulp, hemp pulp, sisal pulp, esparto, etc. These pulps may be used alone or in combination of multiple types in any ratio.
  • the tipping paper 15 may be made up of one sheet, or may be made up of multiple sheets or more.
  • an electrically heated tobacco product also simply referred to as a "method for manufacturing an electrically heated tobacco product"
  • an electrically heated tobacco product comprising an electrically heated device having a heater element, a battery unit that serves as a power source for the heater element, and a control unit for controlling the heater element, and a tobacco product that is inserted so as to come into contact with the heater element, the tobacco product being manufactured by the above-mentioned method for manufacturing a tobacco product.
  • the electrically heated tobacco product may be configured to heat the outer peripheral surface of the tobacco product 10 as shown in Fig.
  • the electrically heated device 20 shown in Figs. 2 and 3 is provided with an air introduction hole, but this is not shown here.
  • the electrically heated tobacco product 30 will be described with reference to Fig. 3. Note that for the tobacco product 10 in Figs. 2 and 3, some of the reference symbols representing the components shown in Fig. 1 will be omitted.
  • the electrically heated tobacco product 30 is used by inserting the tobacco product 10 described above into the heater member 21 arranged inside the electrically heated device 20 so that the tobacco product 10 comes into contact with the heater member 21 .
  • the electrically heated device 20 has a battery unit 22 and a control unit 23 inside a body 24 made of, for example, resin.
  • a body 24 made of, for example, resin.
  • the heater member 21 of the electrically heated device 20 generates heat under the control of the control unit 23. The heat is transmitted to the tobacco rod portion 11 of the tobacco product 10, causing the aerosol base material, flavor components, and the like contained in the tobacco filler of the tobacco rod portion 11 to volatilize.
  • the heater member 21 may be, for example, a sheet heater, a flat heater, or a cylindrical heater.
  • the sheet heater is a flexible sheet-shaped heater, and examples thereof include a heater including a film (thickness: about 20 to 225 ⁇ m) of a heat-resistant polymer such as polyimide.
  • the flat heater is a rigid flat heater (thickness: about 200 to 500 ⁇ m), and examples thereof include a heater having a resistance circuit on a flat substrate and using that part as a heat generating part.
  • the cylindrical heater is a hollow or solid cylindrical heater (thickness: about 200 to 500 ⁇ m), and examples thereof include a heater having a resistance circuit on the outer circumferential surface of a metal cylinder and using that part as a heat generating part.
  • examples thereof include a rod-shaped heater or a cone-shaped heater made of metal or the like having a resistance circuit inside and using that part as a heat generating part.
  • the cross-sectional shape of the cylindrical heater may be a circle, an ellipse, a polygon, a polygon with rounded corners, or the like.
  • the tobacco filler and the like can be heated by supplying power to the inductor by the control unit 23 and heating the susceptor by induction heating.
  • the control unit 23 supplies power to the microwave generator and the tobacco filler and the like in the tobacco rod portion 11 can be heated by microwave heating.
  • the above-mentioned sheet-shaped heater, flat plate heater, and cylindrical heater can be used.
  • the above-mentioned flat plate heater, column heater, and cone heater can be used.
  • the length of the heater member 21 in the major axis direction can be within the range of L ⁇ 5.0 mm, where the length of the tobacco rod portion 11 in the major axis direction is L mm.
  • the length of the heater member 21 in the major axis direction is preferably L mm or more, and from the viewpoint of suppressing the generation of components that have an undesirable effect on the flavor, etc., the length is preferably L+0.5 mm or less, L+1.0 mm or less, L+1.5 mm or less, L+2.0 mm or less, L+2.5 mm or less, L+3.0 mm or less, L+3.5 mm or less, L+4.0 mm or less, L+4.5 mm or less, or L+5.0 mm or less.
  • the heating intensity such as the heating time and heating temperature of the tobacco product 10 by the heater member 21, can be preset for each electrically heated tobacco product 30.
  • the tobacco product 10 can be heated until the temperature of the outer circumferential surface of the portion of the tobacco product 10 inserted into the electrically heated device 20 reaches X (°C), and thereafter, the temperature can be preset to be maintained at a constant temperature of X (°C) or less.
  • the above X (°C) is preferably 80°C or more and 400°C or less.
  • it can be 80°C, 90°C, 100°C, 110°C, 120°C, 130°C, 140°C, 150°C, 160°C, 170°C, 180°C, 190°C, 200°C, 210°C, 220°C, 230°C, 240°C, 250°C, 260°C, 270°C, 280°C, 290°C, 300°C, 310°C, 320°C, 330°C, 340°C, 350°C, 360°C, 370°C, 380°C, 390°C, or 400°C.
  • the opening V in the cooling segment 12 is preferably located closer to the mouth end than the end of the area of the cooling segment 12 that comes into contact with the electrically heated device 20, from the viewpoint of promoting the inflow of air from the outside and suppressing the retention of components and air generated by heating within the cooling segment 12.
  • the insertion opening for the tobacco product 10 in the electrically heated device 20 may be tapered to make it easier to insert the tobacco product 10.
  • a method for producing an oral pouch product is a method for producing an oral pouch product having an oral composition and a pouch for packaging the oral composition, comprising a step of adding a plant extract produced by the above-mentioned method for producing a plant extract to the oral composition.
  • the step of adding the plant extract is not particularly limited as long as it is provided at any stage in the production of the oral pouch product where the plant extract can be added to the oral composition.
  • the plant extract may be added together with other raw materials in the production of the oral composition in the oral composition production step described later, or the plant extract may be added to the produced oral composition, or the plant extract may be added to the oral composition through the packaging material in the packaging step described later after the oral composition is packaged in the packaging material.
  • the plant extract may also be added to the dried residue obtained when extracting the tobacco plant, and the residue may be included in the oral composition.
  • the extraction residue obtained when the tobacco plant is used as the plant in the extraction step in the production of the above-mentioned plant extract can be used, and from the viewpoint of such reusability, it is preferable to produce the plant extract using the tobacco plant.
  • the plant extract When the plant extract is added to the above-mentioned dried residue, other ingredients such as flavoring, pH adjuster, and in some cases emulsifier can be added together to produce the oral composition, and then the composition can be packaged in a pouch to produce the oral pouch product.
  • other ingredients such as flavoring, pH adjuster, and in some cases emulsifier can be added together to produce the oral composition, and then the composition can be packaged in a pouch to produce the oral pouch product.
  • the type of plant is not particularly limited, and for example, plant-derived flavoring components can be added to the oral pouch product, but in particular, when making a smokeless tobacco product, it is preferable to use tobacco plants from the viewpoint of controlling the amount of nicotine.
  • tobacco plants can be added to the oral pouch product as a plant extract using tobacco plants as plants, or the nicotine component described below can be added, or both of these can be used, but from the viewpoint of controlling the amount of nicotine and easy handling, it is preferable to add it as a plant extract using at least tobacco plants as plants.
  • the amount of plant extract added per 100 parts by weight of the oral composition is usually 0.1 parts by weight or more, preferably 3 parts by weight or more, more preferably 5 parts by weight or more, and even more preferably 10 parts by weight or more, and is usually 50 parts by weight or less, preferably 40 parts by weight or less, more preferably 35 parts by weight or less, and even more preferably 20 parts by weight or less.
  • the method for producing the oral pouch product according to the present embodiment can be a known method, except for the step of adding the above-mentioned plant extract.
  • the production process excluding the step of adding the above-mentioned plant extract may include, for example, the oral composition production process and packaging process shown below.
  • the method for producing the oral pouch product may include a step of producing an oral composition.
  • the method for producing the oral composition is not particularly limited, and a known method can be applied. An example of the method is shown below. First, a nicotine source, a base material as an optional ingredient, and the like are mixed in a mixer to obtain a mixture. If necessary, water may be added and heating may be performed. Next, optional ingredients such as fragrance and moisturizer are added, and the mixture is further stirred and mixed to obtain a mixture. Furthermore, the pre-heated mixture may be subjected to a heating treatment as necessary. After preparing the mixture, the mixture may be dried (drying step). Then, the mixture may be cooled. The cooling may be natural or by using some cooling means (cooling step). By drying, the water content of the mixture can be adjusted to a desired value between 5 and 55% by weight. This makes it easy to adjust the water content of the oral composition as the target product.
  • the oral composition obtained in the oral composition preparation step is packaged with a packaging agent to obtain a pouch product (packaging step).
  • the packaging method is not particularly limited, and any known method can be applied, for example, a method in which the oral composition is placed in a bag-shaped nonwoven fabric and then sealed, or other known methods can be used.
  • water may be further added to obtain an oral composition having a desired water content (water addition step). For example, when the water content of the target oral composition is 50% by weight and the water content of the oral composition obtained in the oral composition preparation step is 15% by weight, the remaining 35% by weight of water is added.
  • the oral pouch product is, for example, an oral pouch product having an oral composition containing a substrate and a pouch in which the oral composition is packaged.
  • the composition of the oral composition is not particularly limited as long as it contains at least a base material.
  • the oral composition is a general term for any material contained in the pouch.
  • the oral composition is not a liquid, and is, for example, a solid or gel material, or a mixture thereof.
  • the oral composition contains a base material.
  • the type of base material is not particularly limited, and polysaccharides, porous structures, etc. that can adsorb and retain moisture can be used.
  • the base material is preferably one or more selected from the group consisting of cellulose, microcrystalline cellulose (MCC), spherical cellulose, and porous cellulose, and is more preferably cellulose in terms of the freedom of adjusting the bulk density of the oral composition and in terms of exhibiting white color. These substances may be used alone or in combination of two or more types in any type and ratio.
  • the oral composition may contain nicotine, and the manner in which nicotine is contained is not particularly limited. Nicotine may be added by adding a plant extract using a tobacco plant as the plant, but instead of or in addition to this, nicotine may be contained in the oral composition by a method other than the plant extract.
  • the manner in which nicotine is contained by a method other than the addition of a plant extract is not particularly limited, and for example, it may be contained by using an extract of a nicotine-containing substance such as tobacco leaves, processed tobacco leaves, or tobacco leaves, or nicotine may be contained as a compound, or a nicotine-carrying substance such as a nicotine salt or stabilized nicotine (for example, nicotine supported on an ion exchange resin) may be contained.
  • the nicotine-carrying substance may be a substance in which nicotine is supported on an ion exchange resin as described above, and when nicotine is supported on an ion exchange resin, an ion exchange resin is used as a carrier.
  • examples of the processed product include tobacco powder obtained by pulverizing tobacco leaves.
  • the tobacco powder may include shreds, fine powder, fibers, etc. of dried tobacco leaf lamina, and may be prepared by the following method.
  • tobacco leaves may include mesophyll (lamina), leaf veins (stems), and roots.
  • the tobacco filler may include elements derived from the midrib and roots of tobacco leaves in addition to the tobacco powder basically obtained from the lamina of tobacco leaves.
  • the oral composition may contain substances other than the above-mentioned base material and nicotine (other substances), such as humectants, pH adjusters, gelling agents, gelling auxiliary components, water, flavors, sweeteners, bitterness suppressants, whitening agents, emulsifiers, etc.
  • substances other than the above-mentioned base material and nicotine such as humectants, pH adjusters, gelling agents, gelling auxiliary components, water, flavors, sweeteners, bitterness suppressants, whitening agents, emulsifiers, etc.
  • the content of other substances in the oral composition is not particularly limited, and for substances for which no preferred content is specified, the composition can be adjusted appropriately according to the product design.
  • the pouch is not particularly limited, and any known material can be used as long as it can package the oral composition, is insoluble in water, and has permeability to liquids (water, saliva, etc.) and water-soluble components in the oral composition.
  • Examples of pouch materials include cellulose-based nonwoven fabrics, and commercially available nonwoven fabrics may also be used. A sheet made of such a material is molded into a bag shape, the oral composition is poured into the bag, and the bag is sealed by heat sealing or other means to produce a pouch product.
  • the pouch may contain any ingredient, for example, ingredients for adjusting the scent or taste, flavorings, additives, tobacco extracts, colorings, etc.
  • the manner in which these ingredients are contained is not particularly limited, and examples include coating the pouch surface with the ingredient, impregnating the pouch with the ingredient, and, in the case of a pouch made of fibers, containing the ingredient in the fibers.
  • the appearance of the pouch is not particularly limited, and may be not only non-transparent but also translucent or transparent, in which case the oral composition packaged in the pouch can be seen through.
  • the pouch product there are no particular limitations on the pouch product, so long as it comprises the oral composition and the pouch in which the oral composition is packaged (the oral composition is enclosed in the pouch).
  • the size and weight of the pouch product are not particularly limited, and the size of the pouch product before use may be such that the long side is 25 mm or more and 40 mm or less, or 28 mm or more and 38 mm or less, and the short side is 10 mm or more and 20 mm or less, or 14 mm or more and 18 mm or less.
  • the weight of the pouch product before use may be such that the long side is 0.1 g or more and 2.0 g or less, or 0.3 g or more and 1.0 g or less.
  • the weight ratio of the oral composition to the total weight of the pouch product is not particularly limited, but is usually 80% by weight or more, preferably 85% by weight or more, and more preferably 90% by weight or more, and is usually 99% by weight or less, preferably 97% by weight or less, and more preferably 95% by weight or less.
  • the measurement sample is kept in an environment similar to the environment in which the measurement will be performed for at least 48 hours before the measurement.
  • the measurement temperature, measurement humidity, and measurement pressure are normal temperature (22 ⁇ 2°C), normal humidity (60 ⁇ 5% RH), and normal pressure (atmospheric pressure) unless otherwise specified.
  • oral pouch products are not particularly limited, but examples include oral tobacco such as chewing tobacco, snuff, and compressed tobacco, and nicotine-containing preparations called nicotine pouches, etc. These are inserted between the lips and gums in the oral cavity to enjoy the taste and aroma.
  • the filtrate (after metal membrane filtration) was further filtered using a cross-flow filter (Lab Stak M2O manufactured by Alfa-Laval) under the following conditions to obtain a filtrate (after cross-flow filtration).
  • Average pore size of the filter 100,000 Da
  • Filtration membrane thickness 0.1 ⁇ m
  • Membrane surface flow rate relative to the average pore size of the filtration membrane 50 mL/min cm2 [Dialysis process]
  • the filtrate (after cross-flow filtration) was subjected to electrodialysis using an electrodialysis device (MICRO ACILYZER S3 manufactured by ASTOM CORPORATION) under the following conditions to obtain a plant extract.
  • the salt concentration was measured using a saline refractometer (Master-S28 ⁇ manufactured by Atago Co., Ltd.). That is, the sample solution after salt addition, the salting-out solution after the salting-out step, the filtrate after metal membrane filtration (after metal membrane filtration), the filtrate after cross-flow filtration (after cross-flow filtration), or the plant extract after the dialysis step were dropped into the saline refractometer and the scale value was read to determine the salt concentration.
  • the measurement results are shown in Table 1. From Table 1, it is seen that the salt concentration, which was 5% by weight, decreased with each filtration step, and it was possible to remove all of the salt after electrodialysis.
  • the concentrated phase which is the side to which electrolytes move by electrodialysis from the sample phase containing the plant extract
  • a water quality simplified measuring device Pack Test Nitric Acid, manufactured by Kyoritsu Rikagaku Kenkyusho Co., Ltd.
  • the evaluation results are shown in Table 3 and FIG. 4. At each of the above time points, the salt concentration and electrical conductivity of the aqueous phase and the supernatant hexane phase were measured.
  • the salt concentration and electrical conductivity were measured in the same manner as in Experiment 1.
  • the measurement results are shown in Table 3.
  • the Brix of each salting-out solution was measured in the same manner as in Experiment 2.
  • the measurement results are shown in Table 3.
  • "-" indicates that the measurement was not performed.
  • the method for producing a plant extract according to this embodiment can reduce the concentration of nitrate ions in the plant extract.
  • the present invention can provide a method for producing a plant extract, which efficiently separates and removes impurities such as proteins from a plant extract to obtain a plant extract. Furthermore, by using this plant extract, it is possible to provide a method for producing a tobacco product or oral pouch product, which can reduce the amount of impurities such as proteins while controlling the amount of desired components.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Cigarettes, Filters, And Manufacturing Of Filters (AREA)
PCT/JP2022/046283 2022-12-15 2022-12-15 植物エキスの製造方法 Ceased WO2024127603A1 (ja)

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PCT/JP2022/046283 WO2024127603A1 (ja) 2022-12-15 2022-12-15 植物エキスの製造方法
JP2024564083A JPWO2024127603A1 (https=) 2022-12-15 2022-12-15
KR1020257021886A KR20250116711A (ko) 2022-12-15 2022-12-15 식물 추출물의 제조 방법
EP22968513.6A EP4635596A1 (en) 2022-12-15 2022-12-15 Method for producing plant extract
CN202280102486.2A CN120529955A (zh) 2022-12-15 2022-12-15 用于生产植物提取物的方法

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JPH0322971A (ja) * 1989-06-20 1991-01-31 Calpis Food Ind Co Ltd:The ビフィズス菌増殖物質の精製法及び増殖物質
US5235992A (en) 1991-06-28 1993-08-17 R. J. Reynolds Tobacco Company Processes for producing flavor substances from tobacco and smoking articles made therewith
WO2004098323A1 (ja) 2003-05-06 2004-11-18 Japan Tobacco Inc. 再生タバコ材の製造方法
CN111035697A (zh) * 2019-12-31 2020-04-21 格律药业有限公司 一种药物组合物及其应用和纳米颗粒的制备方法和茶叶提取物的提取工艺及茶叶提取物

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US3424171A (en) 1966-08-15 1969-01-28 William A Rooker Tobacco aromatics enriched nontobacco smokable product and method of making same
US3959246A (en) 1974-07-08 1976-05-25 The United States Of America As Represented By The Secretary Of Agriculture Preparation of soluble edible protein from leafy green crops
US4150677A (en) 1977-01-24 1979-04-24 Philip Morris Incorporated Treatment of tobacco
US4302308A (en) 1980-03-05 1981-11-24 Philip Morris, Inc. Method for electrolytic denitration of tobacco
US4727889A (en) 1986-12-22 1988-03-01 R. J. Reynolds Tobacco Company Tobacco processing
US4941484A (en) 1989-05-30 1990-07-17 R. J. Reynolds Tobacco Company Tobacco processing
US5301694A (en) 1991-11-12 1994-04-12 Philip Morris Incorporated Process for isolating plant extract fractions
US9301544B2 (en) 2013-03-14 2016-04-05 R.J. Reynolds Tobacco Company Protein-enriched tobacco-derived composition
CN104351943B (zh) 2014-09-09 2016-02-10 安徽中烟工业有限责任公司 一种利用电渗析法降低再造烟叶浓缩液中tsna和硝酸盐含量的方法
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JPH0322971A (ja) * 1989-06-20 1991-01-31 Calpis Food Ind Co Ltd:The ビフィズス菌増殖物質の精製法及び増殖物質
US5235992A (en) 1991-06-28 1993-08-17 R. J. Reynolds Tobacco Company Processes for producing flavor substances from tobacco and smoking articles made therewith
WO2004098323A1 (ja) 2003-05-06 2004-11-18 Japan Tobacco Inc. 再生タバコ材の製造方法
CN111035697A (zh) * 2019-12-31 2020-04-21 格律药业有限公司 一种药物组合物及其应用和纳米颗粒的制备方法和茶叶提取物的提取工艺及茶叶提取物

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See also references of EP4635596A1

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