WO2024087660A1 - Low-angle-effect multi-notch color separation filter for multi-channel fluorescence detection - Google Patents
Low-angle-effect multi-notch color separation filter for multi-channel fluorescence detection Download PDFInfo
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 21
- 238000000926 separation method Methods 0.000 title claims abstract description 20
- 230000028161 membrane depolarization Effects 0.000 claims abstract description 28
- 239000000463 material Substances 0.000 claims abstract description 21
- 230000000694 effects Effects 0.000 claims abstract description 20
- 230000003287 optical effect Effects 0.000 claims abstract description 13
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 239000011521 glass Substances 0.000 claims abstract description 5
- 239000010408 film Substances 0.000 claims description 19
- 239000010409 thin film Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims 2
- ZKATWMILCYLAPD-UHFFFAOYSA-N niobium pentoxide Chemical compound O=[Nb](=O)O[Nb](=O)=O ZKATWMILCYLAPD-UHFFFAOYSA-N 0.000 claims 2
- 229910052681 coesite Inorganic materials 0.000 claims 1
- 229910052906 cristobalite Inorganic materials 0.000 claims 1
- 239000000377 silicon dioxide Substances 0.000 claims 1
- 235000012239 silicon dioxide Nutrition 0.000 claims 1
- 229910052682 stishovite Inorganic materials 0.000 claims 1
- PBCFLUZVCVVTBY-UHFFFAOYSA-N tantalum pentoxide Inorganic materials O=[Ta](=O)O[Ta](=O)=O PBCFLUZVCVVTBY-UHFFFAOYSA-N 0.000 claims 1
- 229910052905 tridymite Inorganic materials 0.000 claims 1
- 239000012528 membrane Substances 0.000 abstract description 9
- 230000005540 biological transmission Effects 0.000 abstract description 4
- 230000005284 excitation Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 238000001228 spectrum Methods 0.000 description 5
- 229910004298 SiO 2 Inorganic materials 0.000 description 4
- 230000010287 polarization Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- 229910010413 TiO 2 Inorganic materials 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 101001121408 Homo sapiens L-amino-acid oxidase Proteins 0.000 description 1
- 102100026388 L-amino-acid oxidase Human genes 0.000 description 1
- 101100012902 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) FIG2 gene Proteins 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007847 digital PCR Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/20—Filters
- G02B5/28—Interference filters
Definitions
- the invention relates to the field of optical filters, and in particular to a low-angle effect multi-notch color separation filter for multi-channel fluorescence detection.
- Color separation filters are mainly used to separate excitation light and emission light in fluorescence detection systems.
- Typical multi-channel fluorescence detection applications such as multi-channel PCR detection, digital PCR detection and gene sequencing require simultaneous detection of multiple targets.
- the conventional multi-channel fluorescence detection method is that multiple single-channel excitation lights simultaneously excite a corresponding number of single-channel emission lights.
- the patent with publication number CN113917587A discloses a multi-passband depolarization dichroic filter for multi-color fluorescence detection, which is composed of a substrate and a high-steepness depolarization multi-passband dichroic mirror film system; the film system structure is: S
- the invention has high steepness and depolarization spectral characteristics at 45° incidence, and the position of each adaptive band and the depolarization type can be flexibly adjusted.
- the above-mentioned filter has a narrow passband range and a wide cutoff range, and is not suitable for a multi-channel fluorescence detection system in which a single excitation channel excites multiple emission light channels. In addition, it does not have a low-angle effect and does not have good wide-angle applicability.
- the present invention provides a multi-notch color filter with low angle effect for multi-channel fluorescence detection.
- the multi-notch color filter has a low angle effect when the light is incident at 45°, has a wide transmission range, high steepness and depolarization characteristics, can obtain multiple notch bands and the number of notches is adjustable.
- the present invention adopts the following solutions:
- a low-angle effect multi-notch color separation filter for multi-channel fluorescence detection comprises a basic film system plated on a substrate, wherein the film system structure is: S
- the optical thicknesses of H and L are both ⁇ 0 /4, wherein ⁇ 0 is the center wavelength of the filter.
- the H is made of TiO 2 , Nb 2 O 5 or Ta 2 O 5 optical thin film material; and the L is made of SiO 2 optical thin film material.
- the depolarization coefficient satisfies b+c ⁇ 1.
- the present invention has the following beneficial effects:
- the multi-notch color filter of the present invention has a low-angle effect when the light is incident at 45°, has a wide transmission range, high steepness and depolarization characteristics, can obtain multiple notch bands and the number of notches is adjustable.
- the low-angle effect multi-notch color filter film system for multi-channel fluorescence detection of the present invention has the following characteristics:
- the two-material design makes the membrane layer regular and thin, and has high manufacturability.
- FIG1 is a structural diagram of a membrane system of the present invention.
- FIG2 is a full spectrum diagram of the 45° five-notch color separation filter of the present invention.
- FIG. 3 is a diagram showing the overall full spectrum of the 45° triple-notch color separation filter of the present invention.
- the purpose of the present invention is to provide a multi-notch color filter with low angle effect for multi-channel fluorescence detection, which has low angle effect when light is incident at 45°, wide transmission range, high steepness and depolarization characteristics, and can obtain multiple notch bands and the number of notches is adjustable.
- the present invention is a low-angle effect multi-notch color filter for multi-channel fluorescence detection, including a basic film system plated on a substrate, and its film system structure is: S
- H and L are both ⁇ 0 /4, where ⁇ 0 is the center wavelength of the filter.
- H is made of TiO 2 , Nb 2 O 5 or Ta 2 O 5 optical thin film material, and L is made of SiO 2 optical thin film material.
- a is 1 for double notch, 2 for triple notch, 3 for quadruple notch, and 4 for quintuple notch.
- the depolarization coefficient satisfies b+c ⁇ 1.
- the spectrum requirement is: light incident at 45°, five notch color filters;
- A is adopted.
- the high refractive index material Ta 2 O 5 and the low refractive index material SiO 2 are selected.
- the depolarization coefficient is fine-tuned by computer software to achieve a smaller polarization separation.
- the Conjugate and Optimac combined optimization methods are used to set the target value using computer software, and the final film system is obtained based on the actual calculation results.
- the thickness distribution of each layer in the design is shown in Table 1, and the design curve is shown in Figure 2.
- the light is incident at 45°, and a three-notch color filter is used;
- A is adopted.
- the high refractive index material Ta 2 O 5 and the low refractive index material SiO 2 are selected.
- the depolarization coefficient is fine-tuned by computer software to achieve a smaller polarization separation.
- the Conjugate and Optimac combined optimization methods are used to set the target value using computer software, and the final film system is obtained based on the actual calculation results.
- the thickness distribution of each layer in the design is shown in Table 2, and the design curve is shown in Figure 3.
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- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Filters (AREA)
Abstract
The present invention relates to the field of optical filters, and in particular to a low-angle-effect multi-notch color separation filter for multi-channel fluorescence detection. The low-angle-effect multi-notch color separation filter comprises a base membrane system, which is plated on a substrate, the membrane system structure of the base membrane system being S|k(0.5LcHbL2aHbLcH0.5L)^m/A, wherein S is a glass substrate; A is air; H is a high-refractive-index material; L is a low-refractive-index material; k is a wavelength coefficient, and a notch position can be adjusted and a suitable notch bandwidth can be selected by means of adjusting the wavelength coefficient; a is a notch coefficient, and the number of notches and the degree of angular deflection are adjusted by means of adjusting the notch coefficient; b and c are depolarization coefficients, and the degree of depolarization is adjusted by means of adjusting the depolarization coefficients; and m is the total number of cycles of the membrane system, which determines the steepness and cut-off depth of the membrane system. The present invention has a low angle effect at an incident angle of 45°, and has the characteristics of a wide transmission range, high steepness and depolarization; and a plurality of notch bands can be obtained, and the number of notches is adjustable.
Description
本申请要求于2022年10月27日提交中国专利局、申请号为202211322522.4、发明名称为“多通道荧光检测用低角度效应多陷波分色滤光片”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。This application claims the priority of the Chinese patent application filed with the China Patent Office on October 27, 2022, with application number 202211322522.4 and invention name “Low-angle effect multi-notch color separation filter for multi-channel fluorescence detection”, the entire contents of which are incorporated by reference in this application.
本发明涉及光学滤光片领域，尤其涉及一种多通道荧光检测用低角度效应多陷波分色滤光片。The invention relates to the field of optical filters, and in particular to a low-angle effect multi-notch color separation filter for multi-channel fluorescence detection.
分色滤光片在荧光检测系统中主要是用来分离激发光和发射光。典型的多通道荧光检测应用如多通道PCR检测、数字PCR检测和基因测序中，要求多靶点同时检测，其光路中同时存在多个波长的激发光和多个谱段的荧光以及检焦系统波段，由于通道个数的增多压缩了通道间隔，因此对分色滤光片的陡度和宽角度适应性提出了更高的要求。常规的多通道荧光检测方式是多个单通道激发光同时激发出相应个数的单通道发射光。近期随着多通道荧光检测技术的发展和新型荧光试剂的研制，出现了新的方法，即一个激发光通道激发出多个发射光通道，系统中存在多个这样的激发发射组，分色镜需把多个激发发射组中的激发光和发射光分离。Color separation filters are mainly used to separate excitation light and emission light in fluorescence detection systems. Typical multi-channel fluorescence detection applications such as multi-channel PCR detection, digital PCR detection and gene sequencing require simultaneous detection of multiple targets. There are multiple wavelengths of excitation light and multiple spectral fluorescence and focusing system bands in the optical path. Since the increase in the number of channels compresses the channel interval, higher requirements are placed on the steepness and wide-angle adaptability of the color separation filter. The conventional multi-channel fluorescence detection method is that multiple single-channel excitation lights simultaneously excite a corresponding number of single-channel emission lights. Recently, with the development of multi-channel fluorescence detection technology and the development of new fluorescent reagents, a new method has emerged, that is, one excitation light channel excites multiple emission light channels. There are multiple such excitation emission groups in the system, and the color separation mirror needs to separate the excitation light and emission light in the multiple excitation emission groups.
对于传统的多通道荧光检测方式，公开号为CN113917587A的专利披露了一种多色荧光检测用多通带消偏振二向分色滤光片，由基底及高陡度消偏振多通带二向分色镜膜系构成；膜系结构为：S|[(aL HL bH LH aL)^m]|A；或S|[(aL HLH bL LHHaL)^m]|A；其中：S为玻璃基底；A为空气；H为高折射率材料；L为低折射率材料；H、L均为光学厚度为λ0/4的膜层；a为峰位系数，b为通带消偏振系数，m为基础膜系的总体循环个数。该发明在45°入射时具有高陡度、消偏振的光谱特性，且各适配波段位置和消偏振类型可以灵活调整。但上述滤光片其通带范围窄，截止范围宽，不适用于单激发通道激发多个发射光通道这种方式的多通道荧光检测系统，并且其不具备低角度效应，没有好的宽角度适用性。
For the traditional multi-channel fluorescence detection method, the patent with publication number CN113917587A discloses a multi-passband depolarization dichroic filter for multi-color fluorescence detection, which is composed of a substrate and a high-steepness depolarization multi-passband dichroic mirror film system; the film system structure is: S|[(aL HL bH LH aL)^m]|A; or S|[(aL HLH bL LHHaL)^m]|A; where: S is a glass substrate; A is air; H is a high refractive index material; L is a low refractive index material; H and L are both film layers with an optical thickness of λ 0 /4; a is a peak position coefficient, b is a passband depolarization coefficient, and m is the total number of cycles of the basic film system. The invention has high steepness and depolarization spectral characteristics at 45° incidence, and the position of each adaptive band and the depolarization type can be flexibly adjusted. However, the above-mentioned filter has a narrow passband range and a wide cutoff range, and is not suitable for a multi-channel fluorescence detection system in which a single excitation channel excites multiple emission light channels. In addition, it does not have a low-angle effect and does not have good wide-angle applicability.
发明内容Summary of the invention
针对现有技术的不足之处，本发明提供一种多通道荧光检测用低角度效应多陷波分色滤光片。该多陷波分色滤光片在光线以45°入射时具备低角度效应，具有透射范围宽、陡度高及消偏振特性，可获得多个陷波带且陷波个数可调。In view of the shortcomings of the prior art, the present invention provides a multi-notch color filter with low angle effect for multi-channel fluorescence detection. The multi-notch color filter has a low angle effect when the light is incident at 45°, has a wide transmission range, high steepness and depolarization characteristics, can obtain multiple notch bands and the number of notches is adjustable.
为解决上述技术问题，本发明采用如下方案：In order to solve the above technical problems, the present invention adopts the following solutions:
一种多通道荧光检测用低角度效应多陷波分色滤光片，包括基片上镀制的基础膜系，其膜系结构为：S|k(0.5LcHbL2aHbLcH0.5L)^m|A；其中：S为玻璃基底；A为空气；H为高折射率材料；L为低折射率材料，k为波长系数，调整波长系数可调整陷波位置和选择合适的陷波带宽；a为陷波系数，通过调整陷波系数调节陷波数量和角度偏移程度；b、c为消偏振系数，通过调整消偏振系数调整消偏振程度；m为膜系的总体循环个数，决定膜系的陡度和截止深度。A low-angle effect multi-notch color separation filter for multi-channel fluorescence detection comprises a basic film system plated on a substrate, wherein the film system structure is: S|k(0.5LcHbL2aHbLcH0.5L)^ m |A; wherein: S is a glass substrate; A is air; H is a high refractive index material; L is a low refractive index material; k is a wavelength coefficient, and the notch position can be adjusted and a suitable notch bandwidth can be selected by adjusting the wavelength coefficient; a is a notch coefficient, and the notch number and angle deviation degree can be adjusted by adjusting the notch coefficient; b and c are depolarization coefficients, and the depolarization degree can be adjusted by adjusting the depolarization coefficient; and m is the total number of cycles of the film system, which determines the steepness and cutoff depth of the film system.
进一步地，H与L的光学厚度均为λ0/4；其中，λ0为滤光片中心波长。Furthermore, the optical thicknesses of H and L are both λ 0 /4, wherein λ 0 is the center wavelength of the filter.
进一步地，所述H采用TiO2、Nb2O5或Ta2O5光学薄膜材料；所述L采用SiO2光学薄膜材料。Furthermore, the H is made of TiO 2 , Nb 2 O 5 or Ta 2 O 5 optical thin film material; and the L is made of SiO 2 optical thin film material.
进一步地，所述a数值取1为双陷波；a数值取2为三陷波，a数值取3为四陷波；a数值取4为五陷波。Furthermore, a value of 1 represents double notch; a value of 2 represents triple notch; a value of 3 represents quad notch; and a value of 4 represents quintuple notch.
进一步地，所述消偏振系数满足b+c<1。Furthermore, the depolarization coefficient satisfies b+c<1.
与现有技术相比，本发明的有益效果为：Compared with the prior art, the present invention has the following beneficial effects:
本发明多陷波分色滤光片在光线以45°入射时具备低角度效应，具有透射范围宽、陡度高及消偏振特性，可获得多个陷波带且陷波个数可调。具体地，本发明多通道荧光检测用低角度效应多陷波分色滤光片膜系具备以下特征：The multi-notch color filter of the present invention has a low-angle effect when the light is incident at 45°, has a wide transmission range, high steepness and depolarization characteristics, can obtain multiple notch bands and the number of notches is adjustable. Specifically, the low-angle effect multi-notch color filter film system for multi-channel fluorescence detection of the present invention has the following characteristics:
(1)光线45°入射时使用；(1) Use when the light is incident at 45°;
(2)低角度偏移效应，入射角范围(42～48°)，光谱波长偏移小于12nm；(2) Low angle deviation effect, within the incident angle range (42-48°), the spectral wavelength deviation is less than 12 nm;
(3)陷波数量可调；(3) The number of notches is adjustable;
(4)高透过(T>90％)带范围远大于陷波范围；(4) The high transmittance (T>90%) band range is much larger than the notch range;
(5)滤光片通带和反射带之间的两个过渡带，同时具备10nm以内高陡
度，所有过渡带T50％偏振分离度小于5nm；(5) Two transition bands between the filter passband and reflection band, with high steepness within 10nm The T50% polarization separation of all transition bands is less than 5nm;
(6)采用两种材料设计，膜层规整无极薄层，具有较高的可制备性。(6) The two-material design makes the membrane layer regular and thin, and has high manufacturability.
说明书附图Instruction Manual
下面结合附图和具体实施方式对本发明作进一步说明。本发明的保护范围不仅局限于下列内容的表述。The present invention is further described below in conjunction with the accompanying drawings and specific implementation methods. The protection scope of the present invention is not limited to the following description.
图1为本发明膜系结构图；FIG1 is a structural diagram of a membrane system of the present invention;
图2为本发明45°五陷波分色滤光片整体全光谱图；FIG2 is a full spectrum diagram of the 45° five-notch color separation filter of the present invention;
图3为本发明45°三陷波分色滤光片整体全光谱图。FIG. 3 is a diagram showing the overall full spectrum of the 45° triple-notch color separation filter of the present invention.
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
本发明的目的是提供一种多通道荧光检测用低角度效应多陷波分色滤光片，该多陷波分色滤光片在光线以45°入射时具备低角度效应，具有透射范围宽、陡度高及消偏振特性，可获得多个陷波带且陷波个数可调。。The purpose of the present invention is to provide a multi-notch color filter with low angle effect for multi-channel fluorescence detection, which has low angle effect when light is incident at 45°, wide transmission range, high steepness and depolarization characteristics, and can obtain multiple notch bands and the number of notches is adjustable.
为使本发明的上述目的、特征和优点能够更加明显易懂，下面结合附图和具体实施方式对本发明作进一步详细的说明。In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.
如图1所示，本发明一种多通道荧光检测用低角度效应多陷波分色滤光片，包括基片上镀制的基础膜系，其膜系结构为：S|k(0.5LcHbL2aHbLcH0.5L)^m|A；其中：S为玻璃基底；A为空气；H为高折射率材料；L为低折射率材料，k为波长系数，调整波长系数可调整陷波位置和选择合适的陷波带宽；a为陷波系数，通过调整陷波系数调节陷波数量和角度偏移程度；b、c为消偏振系数，通过调整消偏振系数调整消偏振程度；m为膜系的总体循环个数，决定膜系的陡度和截止深度。As shown in Figure 1, the present invention is a low-angle effect multi-notch color filter for multi-channel fluorescence detection, including a basic film system plated on a substrate, and its film system structure is: S|k(0.5LcHbL2aHbLcH0.5L)^ m |A; wherein: S is a glass substrate; A is air; H is a high refractive index material; L is a low refractive index material, k is a wavelength coefficient, and the wavelength coefficient can be adjusted to adjust the notch position and select a suitable notch bandwidth; a is a notch coefficient, and the notch number and angle deviation degree are adjusted by adjusting the notch coefficient; b and c are depolarization coefficients, and the depolarization degree is adjusted by adjusting the depolarization coefficient; m is the total number of cycles of the film system, which determines the steepness and cutoff depth of the film system.
其中，H与L的光学厚度均为λ0/4；其中，λ0为滤光片中心波长。所述H采用TiO2、Nb2O5或Ta2O5光学薄膜材料；所述L采用SiO2光学薄膜材料。
The optical thicknesses of H and L are both λ 0 /4, where λ 0 is the center wavelength of the filter. H is made of TiO 2 , Nb 2 O 5 or Ta 2 O 5 optical thin film material, and L is made of SiO 2 optical thin film material.
进一步地，所述a数值取1为双陷波；a数值取2为三陷波，a数值取3为四陷波；a数值取4为五陷波。所述消偏振系数满足b+c<1。Furthermore, the value of a is 1 for double notch, 2 for triple notch, 3 for quadruple notch, and 4 for quintuple notch. The depolarization coefficient satisfies b+c<1.
多通道荧光用低角度效应多陷波分色滤光片具备如下技术特点：The low-angle effect multi-notch color separation filter for multi-channel fluorescence has the following technical features:
(1)光线以45°入射时使用。(1) Use when light is incident at 45°.
(2)宽角度使用范围，低角度偏移效应。(2) Wide angle range of use and low angle deviation effect.
(3)多个(2个以上)反射带。(3) Multiple (more than 2) reflection bands.
(4)除反射带外具有宽波段范围的高透过带。(4) In addition to the reflection band, it has a high transmittance band over a wide wavelength range.
(5)滤光片通带和反射带之间的两个过渡带，同时具备高陡度和低偏振分离度。(5) Two transition bands between the filter passband and reflection band, with high steepness and low polarization separation.
实施例1Example 1
如图2所示，以光谱要求：光线45°入射，五陷波分色滤光片；As shown in Figure 2, the spectrum requirement is: light incident at 45°, five notch color filters;
T>90％@380nm-398nm&412nm-440nm&454nm-496nm&510nm-570nm&585nm-672nm&696nm-820nm；T>90%@380nm-398nm&412nm-440nm&454nm-496nm&510nm-570nm&585nm-672nm&696nm-820nm;
T<5％@401nm-408nm&443nm-450nm&498nm-505nm&573nm-580nm&677nm-689nm；42～48°光谱波长偏移小于12nm为例。T<5%@401nm-408nm&443nm-450nm&498nm-505nm&573nm-580nm&677nm-689nm; 42～48° spectrum wavelength shift is less than 12nm as an example.
采用膜系基础结构S|k(0.5LcHbL2aHbLcH0.5L)^m|A。The membrane basic structure S|k(0.5LcHbL2aHbLcH0.5L)^ m |A is adopted.
选择高折射率材料Ta2O5、低折射率材料SiO2。The high refractive index material Ta 2 O 5 and the low refractive index material SiO 2 are selected.
根据陷波个数和角度偏移度确定陷波系数a＝4。The notch coefficient a=4 is determined according to the number of notches and the angle deviation.
根据陷波位置调整波长系数k＝1。The wavelength coefficient k=1 is adjusted according to the notch position.
给出初始消偏振系数b、c，b＝0.18；c＝0.47。The initial depolarization coefficients b and c are given as: b=0.18; c=0.47.
以及合适的循环次数m＝22。And the appropriate number of cycles is m=22.
通过计算机软件微调消偏振系数使其达到更小的偏振分离。The depolarization coefficient is fine-tuned by computer software to achieve a smaller polarization separation.
利用计算机软件对设定目标值采用Conjugate和Optimac组合优化方法，根据实际计算结果，获得最终膜系。设计中各层厚度分布见表一，设计曲线见图2。The Conjugate and Optimac combined optimization methods are used to set the target value using computer software, and the final film system is obtained based on the actual calculation results. The thickness distribution of each layer in the design is shown in Table 1, and the design curve is shown in Figure 2.
表一：各层厚度分布
Table 1: Thickness distribution of each layer
Table 1: Thickness distribution of each layer
实施例2Example 2
如图3所示，以光谱要求：光线以45°入射，三陷波分色滤光片；
As shown in Figure 3, according to the spectrum requirements: the light is incident at 45°, and a three-notch color filter is used;
T>90％@380nm-418nm&438nm-510nm&531nm-661nm&698nm-958nm；T>90%@380nm-418nm&438nm-510nm&531nm-661nm&698nm-958nm;
T<5％@421nm-433nm&514nm-525nm&668nm-689nm为例。Take T<5%@421nm-433nm&514nm-525nm&668nm-689nm as an example.
采用膜系基础结构S|k(0.5LcHbL2aHbLcH0.5L)^m|A。The membrane basic structure S|k(0.5LcHbL2aHbLcH0.5L) ^m |A is adopted.
选择高折射率材料Ta2O5、低折射率材料SiO2。The high refractive index material Ta 2 O 5 and the low refractive index material SiO 2 are selected.
根据陷波个数和角度偏移度确定陷波系数a＝2。The notch coefficient a=2 is determined according to the number of notches and the angle deviation.
根据陷波位置调整波长系数k＝1。The wavelength coefficient k=1 is adjusted according to the notch position.
给出初始消偏振系数b、c，b＝0.2；c＝0.5。The initial depolarization coefficients b and c are given as follows: b = 0.2; c = 0.5.
以及合适的循环次数m＝25。And the appropriate number of cycles is m=25.
通过计算机软件微调消偏振系数使其达到更小的偏振分离。The depolarization coefficient is fine-tuned by computer software to achieve a smaller polarization separation.
利用计算机软件对设定目标值采用Conjugate和Optimac组合优化方法，根据实际计算结果，获得最终膜系。设计中各层厚度分布见表二，设计曲线见图3。The Conjugate and Optimac combined optimization methods are used to set the target value using computer software, and the final film system is obtained based on the actual calculation results. The thickness distribution of each layer in the design is shown in Table 2, and the design curve is shown in Figure 3.
表二：各层厚度分布
Table 2: Thickness distribution of each layer
Table 2: Thickness distribution of each layer
尽管已经示出和描述了本发明的实施例，对于本领域的普通技术人员而言，可以理解在不脱离本发明的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变型，本发明的范围由所附权利要求及其等同物限定。
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims (5)
- 一种多通道荧光检测用低角度效应多陷波分色滤光片，其特征在于，包括基片上镀制的基础膜系，其膜系结构为：S|k(0.5LcHbL2aHbLcH0.5L)^m|A；其中：S为玻璃基底；A为空气；H为高折射率材料；L为低折射率材料，k为波长系数，调整波长系数调整陷波位置和选择合适的陷波带宽；a为陷波系数，通过调整陷波系数调节陷波数量和角度偏移程度；b、c为消偏振系数，通过调整消偏振系数调整消偏振程度；m为膜系的总体循环个数，决定膜系的陡度和截止深度。A low-angle effect multi-notch color filter for multi-channel fluorescence detection, characterized in that it includes a basic film system plated on a substrate, and its film system structure is: S|k(0.5LcHbL2aHbLcH0.5L) ^m |A; wherein: S is a glass substrate; A is air; H is a high refractive index material; L is a low refractive index material, k is a wavelength coefficient, and the wavelength coefficient is adjusted to adjust the notch position and select a suitable notch bandwidth; a is a notch coefficient, and the notch number and angle deviation degree are adjusted by adjusting the notch coefficient; b and c are depolarization coefficients, and the depolarization degree is adjusted by adjusting the depolarization coefficient; m is the total number of cycles of the film system, which determines the steepness and cutoff depth of the film system.
- 根据权利要求1所述的多通道荧光检测用低角度效应多陷波分色滤光片，其特征在于，H与L的光学厚度均为λ0/4；其中，λ0为滤光片中心波长。The low-angle effect multi-notch color separation filter for multi-channel fluorescence detection according to claim 1 is characterized in that the optical thicknesses of H and L are both λ 0 /4; wherein λ 0 is the center wavelength of the filter.
- 根据权利要求2所述的多通道荧光检测用低角度效应多陷波分色滤光片，其特征在于，所述H采用TiO2、Nb2O5或Ta2O5光学薄膜材料；所述L采用SiO2光学薄膜材料。The low-angle effect multi- notch color separation filter for multi-channel fluorescence detection according to claim 2 is characterized in that the H is made of TiO2 , Nb2O5 or Ta2O5 optical thin film material; and the L is made of SiO2 optical thin film material.
- 根据权利要求3所述的多通道荧光检测用低角度效应多陷波分色滤光片，其特征在于，所述a数值取1为双陷波；a数值取2为三陷波，a数值取3为四陷波；a数值取4为五陷波。According to the low-angle effect multi-notch color separation filter for multi-channel fluorescence detection according to claim 3, it is characterized in that the value of a is 1 for double notch; the value of a is 2 for triple notch, the value of a is 3 for quad notch; and the value of a is 4 for quintuple notch.
- 根据权利要求4所述的多通道荧光检测用低角度效应多陷波分色滤光片，其特征在于，所述消偏振系数满足b+c<1。 The low-angle effect multi-notch color separation filter for multi-channel fluorescence detection according to claim 4, characterized in that the depolarization coefficient satisfies b+c<1.
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| JP2004138798A (en) * | 2002-10-17 | 2004-05-13 | Sun Tec Kk | Multilayered dielectric band-pass filter |
| CN102798914A (en) * | 2012-08-03 | 2012-11-28 | 中国科学院上海技术物理研究所 | Method for adjusting out-of-band cut-off width of bandpass filter with multicavity structure |
| CN112230325A (en) * | 2020-10-29 | 2021-01-15 | 沈阳仪表科学研究院有限公司 | Periodic symmetric structure high-order secondary reflection inhibiting short wave pass film system optical filter |
| CN113917587A (en) * | 2021-10-21 | 2022-01-11 | 沈阳仪表科学研究院有限公司 | Multi-passband depolarizing dichroic color filter for multi-color fluorescence detection |
| CN115639638A (en) * | 2022-10-27 | 2023-01-24 | 沈阳仪表科学研究院有限公司 | Low-angle effect multi-notch color separation filter for multi-channel fluorescence detection |
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| US8958156B1 (en) * | 2007-05-30 | 2015-02-17 | Semrock, Inc. | Interference filter for non-zero angle of incidence spectroscopy |
| WO2017070552A1 (en) * | 2015-10-23 | 2017-04-27 | Sharp Gary D | Optical filter with color enhancement |
| WO2018022735A1 (en) * | 2016-07-28 | 2018-02-01 | Ace Ronald S | Spectrally sculpted multiple narrowband filtration for improved human vision |
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| JP2004138798A (en) * | 2002-10-17 | 2004-05-13 | Sun Tec Kk | Multilayered dielectric band-pass filter |
| CN102798914A (en) * | 2012-08-03 | 2012-11-28 | 中国科学院上海技术物理研究所 | Method for adjusting out-of-band cut-off width of bandpass filter with multicavity structure |
| CN112230325A (en) * | 2020-10-29 | 2021-01-15 | 沈阳仪表科学研究院有限公司 | Periodic symmetric structure high-order secondary reflection inhibiting short wave pass film system optical filter |
| CN113917587A (en) * | 2021-10-21 | 2022-01-11 | 沈阳仪表科学研究院有限公司 | Multi-passband depolarizing dichroic color filter for multi-color fluorescence detection |
| CN115639638A (en) * | 2022-10-27 | 2023-01-24 | 沈阳仪表科学研究院有限公司 | Low-angle effect multi-notch color separation filter for multi-channel fluorescence detection |
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