WO2024079759A2 - Bioréacteur de culture cellulaire avec dispositif de collecte de cellules et procédé de collecte de cellules - Google Patents
Bioréacteur de culture cellulaire avec dispositif de collecte de cellules et procédé de collecte de cellules Download PDFInfo
- Publication number
- WO2024079759A2 WO2024079759A2 PCT/IN2023/050942 IN2023050942W WO2024079759A2 WO 2024079759 A2 WO2024079759 A2 WO 2024079759A2 IN 2023050942 W IN2023050942 W IN 2023050942W WO 2024079759 A2 WO2024079759 A2 WO 2024079759A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cell
- bioreactor
- cells
- culture
- harvester
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract description 10
- 238000003306 harvesting Methods 0.000 title abstract description 3
- 238000004113 cell culture Methods 0.000 title 1
- 230000000694 effects Effects 0.000 abstract description 3
- 239000011159 matrix material Substances 0.000 abstract 4
- 230000036461 convulsion Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 19
- 230000035899 viability Effects 0.000 description 4
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- QXAITBQSYVNQDR-UHFFFAOYSA-N amitraz Chemical compound C=1C=C(C)C=C(C)C=1N=CN(C)C=NC1=CC=C(C)C=C1C QXAITBQSYVNQDR-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 210000003501 vero cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/14—Rotation or movement of the cells support, e.g. rotated hollow fibers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
Definitions
- the ⁇ present ⁇ invention ⁇ is ⁇ a ⁇ combination ⁇ of ⁇ Provisional ⁇ Patent ⁇ Application ⁇ No. ⁇ 202221058526 ⁇ Filed ⁇ on ⁇ October ⁇ 13, ⁇ 2022 ⁇ and ⁇ Provisional ⁇ Patent ⁇ Application ⁇ No. ⁇ 202221076880 ⁇ Filed ⁇ on ⁇ December ⁇ 29, ⁇ 2022.
- the ⁇ present ⁇ invention ⁇ is ⁇ generally ⁇ relates ⁇ to ⁇ a ⁇ method ⁇ and ⁇ system ⁇ for ⁇ harvesting ⁇ cells ⁇ grown ⁇ in ⁇ culture ⁇ and ⁇ more ⁇ particularly, ⁇ it ⁇ relates ⁇ to ⁇ a ⁇ cell ⁇ culture ⁇ bioreactor ⁇ with ⁇ cell ⁇ harvester ⁇ for ⁇ harvesting ⁇ cells ⁇ grown ⁇ in ⁇ culture ⁇ by ⁇ applying ⁇ jarring/jerky ⁇ rotation ⁇ motion ⁇ to ⁇ release ⁇ the ⁇ cells
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
La présente invention concerne un système et un procédé de collecte de cellules par détachement des cellules de la porteuse de cellule. Dans la présente invention, l'ensemble matrice de support de cellule (6) tourne conjointement avec l'arbre central (5). Pendant la rotation de l'arbre central (5), les éléments rotatifs (19) de la bague inférieure (8) roulent sur l'arête du moyeu inférieur (9). Lesdits éléments rotatifs (9) produisent un effet vibro-rotatif et amènent l'ensemble matrice de support de cellule (6) à subir une secousse tout en se déplaçant sur les saillies sur le concentrateur inférieur. Ainsi, il applique un effet de vibro-rotation à la matrice (6). En raison de ladite configuration, l'ensemble matrice de support de cellules (6) amène des cellules fixées aux disques (7) à se détacher des disques (7).
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN202221058526 | 2022-10-13 | ||
IN202221058526 | 2022-10-13 | ||
IN202221076880 | 2022-12-29 | ||
IN202221076880 | 2022-12-29 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2024079759A2 true WO2024079759A2 (fr) | 2024-04-18 |
WO2024079759A3 WO2024079759A3 (fr) | 2024-07-04 |
Family
ID=88833858
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IN2023/050942 WO2024079759A2 (fr) | 2022-10-13 | 2023-10-13 | Bioréacteur de culture cellulaire avec dispositif de collecte de cellules et procédé de collecte de cellules |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2024079759A2 (fr) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140030805A1 (en) | 2011-04-15 | 2014-01-30 | Pluristem Ltd. | Methods and systems for harvesting cells |
US20220033751A1 (en) | 2018-10-03 | 2022-02-03 | Pluristem Ltd. | Modular Bioreactor |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPWO2015033616A1 (ja) * | 2013-09-06 | 2017-03-02 | 旭化成株式会社 | 培養容器駆動装置及び培養システム |
-
2023
- 2023-10-13 WO PCT/IN2023/050942 patent/WO2024079759A2/fr unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140030805A1 (en) | 2011-04-15 | 2014-01-30 | Pluristem Ltd. | Methods and systems for harvesting cells |
US20220033751A1 (en) | 2018-10-03 | 2022-02-03 | Pluristem Ltd. | Modular Bioreactor |
Also Published As
Publication number | Publication date |
---|---|
WO2024079759A3 (fr) | 2024-07-04 |
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