WO2024079759A2 - Bioréacteur de culture cellulaire avec dispositif de collecte de cellules et procédé de collecte de cellules - Google Patents

Bioréacteur de culture cellulaire avec dispositif de collecte de cellules et procédé de collecte de cellules Download PDF

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Publication number
WO2024079759A2
WO2024079759A2 PCT/IN2023/050942 IN2023050942W WO2024079759A2 WO 2024079759 A2 WO2024079759 A2 WO 2024079759A2 IN 2023050942 W IN2023050942 W IN 2023050942W WO 2024079759 A2 WO2024079759 A2 WO 2024079759A2
Authority
WO
WIPO (PCT)
Prior art keywords
cell
bioreactor
cells
culture
harvester
Prior art date
Application number
PCT/IN2023/050942
Other languages
English (en)
Inventor
Ravindrakumar Dhirubhai PATEL
Anandprakash Manuprasad JOSHI
Ravikumar Nikunjkumar DARAJI
Original Assignee
Omnibrx Biotechnologies Private Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Omnibrx Biotechnologies Private Limited filed Critical Omnibrx Biotechnologies Private Limited
Publication of WO2024079759A2 publication Critical patent/WO2024079759A2/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/14—Rotation or movement of the cells support, e.g. rotated hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish

Definitions

  • the ⁇ present ⁇ invention ⁇ is ⁇ a ⁇ combination ⁇ of ⁇ Provisional ⁇ Patent ⁇ Application ⁇ No. ⁇ 202221058526 ⁇ Filed ⁇ on ⁇ October ⁇ 13, ⁇ 2022 ⁇ and ⁇ Provisional ⁇ Patent ⁇ Application ⁇ No. ⁇ 202221076880 ⁇ Filed ⁇ on ⁇ December ⁇ 29, ⁇ 2022.
  • the ⁇ present ⁇ invention ⁇ is ⁇ generally ⁇ relates ⁇ to ⁇ a ⁇ method ⁇ and ⁇ system ⁇ for ⁇ harvesting ⁇ cells ⁇ grown ⁇ in ⁇ culture ⁇ and ⁇ more ⁇ particularly, ⁇ it ⁇ relates ⁇ to ⁇ a ⁇ cell ⁇ culture ⁇ bioreactor ⁇ with ⁇ cell ⁇ harvester ⁇ for ⁇ harvesting ⁇ cells ⁇ grown ⁇ in ⁇ culture ⁇ by ⁇ applying ⁇ jarring/jerky ⁇ rotation ⁇ motion ⁇ to ⁇ release ⁇ the ⁇ cells

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un système et un procédé de collecte de cellules par détachement des cellules de la porteuse de cellule. Dans la présente invention, l'ensemble matrice de support de cellule (6) tourne conjointement avec l'arbre central (5). Pendant la rotation de l'arbre central (5), les éléments rotatifs (19) de la bague inférieure (8) roulent sur l'arête du moyeu inférieur (9). Lesdits éléments rotatifs (9) produisent un effet vibro-rotatif et amènent l'ensemble matrice de support de cellule (6) à subir une secousse tout en se déplaçant sur les saillies sur le concentrateur inférieur. Ainsi, il applique un effet de vibro-rotation à la matrice (6). En raison de ladite configuration, l'ensemble matrice de support de cellules (6) amène des cellules fixées aux disques (7) à se détacher des disques (7).
PCT/IN2023/050942 2022-10-13 2023-10-13 Bioréacteur de culture cellulaire avec dispositif de collecte de cellules et procédé de collecte de cellules WO2024079759A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
IN202221058526 2022-10-13
IN202221058526 2022-10-13
IN202221076880 2022-12-29
IN202221076880 2022-12-29

Publications (1)

Publication Number Publication Date
WO2024079759A2 true WO2024079759A2 (fr) 2024-04-18

Family

ID=88833858

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2023/050942 WO2024079759A2 (fr) 2022-10-13 2023-10-13 Bioréacteur de culture cellulaire avec dispositif de collecte de cellules et procédé de collecte de cellules

Country Status (1)

Country Link
WO (1) WO2024079759A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140030805A1 (en) 2011-04-15 2014-01-30 Pluristem Ltd. Methods and systems for harvesting cells
US20220033751A1 (en) 2018-10-03 2022-02-03 Pluristem Ltd. Modular Bioreactor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140030805A1 (en) 2011-04-15 2014-01-30 Pluristem Ltd. Methods and systems for harvesting cells
US20220033751A1 (en) 2018-10-03 2022-02-03 Pluristem Ltd. Modular Bioreactor

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