WO2024061456A1 - Procédé enzymatique de production de l-glufosinate et de ses phosphoesters - Google Patents
Procédé enzymatique de production de l-glufosinate et de ses phosphoesters Download PDFInfo
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- WO2024061456A1 WO2024061456A1 PCT/EP2022/076218 EP2022076218W WO2024061456A1 WO 2024061456 A1 WO2024061456 A1 WO 2024061456A1 EP 2022076218 W EP2022076218 W EP 2022076218W WO 2024061456 A1 WO2024061456 A1 WO 2024061456A1
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- WIPO (PCT)
- Prior art keywords
- homoserine
- seq
- glufosinate
- activated
- polypeptide
- Prior art date
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- 238000006911 enzymatic reaction Methods 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 42
- UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical class OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 claims abstract description 40
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y206/00—Transferases transferring nitrogenous groups (2.6)
- C12Y206/01—Transaminases (2.6.1)
- C12Y206/01001—Aspartate transaminase (2.6.1.1), i.e. aspartate-aminotransferase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La présente invention concerne un procédé catalysé par voie enzymatique pour la production de L-glufosinate ou d'un phosphoester de celui-ci. Le procédé comprend une étape dans laquelle une L-homosérine HA activée est mise en réaction avec un substrat S choisi parmi l'acide méthylphosphinique et les esters d'acide méthylphosphinique. Une aspartate transaminase E1 est utilisée pour la catalyse enzymatique. L'invention permet l'obtention de nouveaux substrats dans la production enzymatique de L-glufosinate et de ses phosphoesters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2022/076218 WO2024061456A1 (fr) | 2022-09-21 | 2022-09-21 | Procédé enzymatique de production de l-glufosinate et de ses phosphoesters |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2022/076218 WO2024061456A1 (fr) | 2022-09-21 | 2022-09-21 | Procédé enzymatique de production de l-glufosinate et de ses phosphoesters |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2024061456A1 true WO2024061456A1 (fr) | 2024-03-28 |
Family
ID=83995440
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2022/076218 WO2024061456A1 (fr) | 2022-09-21 | 2022-09-21 | Procédé enzymatique de production de l-glufosinate et de ses phosphoesters |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2024061456A1 (fr) |
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|---|---|---|---|---|
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