WO2024058656A1 - Procédé de culture d'un acarien, composition comprenant ledit acarien, système de libération dudit acarien et utilisation dudit acarien - Google Patents

Procédé de culture d'un acarien, composition comprenant ledit acarien, système de libération dudit acarien et utilisation dudit acarien Download PDF

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Publication number
WO2024058656A1
WO2024058656A1 PCT/NL2023/050465 NL2023050465W WO2024058656A1 WO 2024058656 A1 WO2024058656 A1 WO 2024058656A1 NL 2023050465 W NL2023050465 W NL 2023050465W WO 2024058656 A1 WO2024058656 A1 WO 2024058656A1
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Prior art keywords
mites
mite
eggs
substrate
range
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PCT/NL2023/050465
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English (en)
Inventor
Evers JOHNNY
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Join Beheer B.V.
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Publication of WO2024058656A1 publication Critical patent/WO2024058656A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/02—Acaricides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides

Definitions

  • the present invention relates to a method for cultivating a mite, a mite composition comprising said mite, a system for releasing said mite, and use of said mite.
  • mite species selected from Mesostigmatid and Prostigmatid predatory species, such as from the family of the Cheyletidae, Cunaxidae, Macrochelidae, Erythraeidae, Laelapidae, Parasitidae, or Tydeidae, receive interest in biological crop protection.
  • a driving force behind the increasing interest of predatory mites is their efficacy to control harmful crop pests and the availability of mass rearing systems to produce them on a commercially relevant scale for an acceptable price. Therefore, the use of predatory mites as an economic alternative to chemical pesticides is enabled.
  • Conventional methods may for example include rearing the predatory mites on life prey in a culture maintained on a carrier, or to rear predatory mites on eggs from Ephestia kuehniella coated on popcorn grains.
  • the present invention aims at obviating or at least reducing the aforementioned problems and to enable efficient and effective cultivation of mites.
  • This objective is achieved with the method for cultivating a mite, comprising feeding a breeding population of individuals of the mite of the family Parasitidae in a substrate, comprising organic material and vermiculite, with individuals of further mites of the family Carpoglyphidae, Acaridae, Suidasiidae, or a combination thereof, and eggs of crustacean and/or eggs of moths.
  • eggs of crustacean may refer to eggs of a single crustacean and/or eggs of multiple crustaceans. Therefore, crustacean may refer crustacean and/or crustaceans throughout this application.
  • feeding includes adding individuals of the further mites of the family Carpoglyphidae, Acaridae, and/or Suidasiidae, and eggs of crustacean and/or eggs of moths to the breeding population.
  • An advantage of the method according to the invention is that efficient and effective cultivation of a mite of the family Parasitidae is achieved.
  • feeding the mites of the family Parasitidae with the further mites of the family Carpoglyphidae, Acaridae, and/or Suidasiidae includes feeding the mites of the family Parasitidae with one or more individual of the further mites of the family Carpoglyphidae, Acaridae, Suidasiidae.
  • Feeding the mites of the family Parasitidae with individuals of further mites of the family Carpoglyphidae, Acaridae, and/or Suidasiidae, and eggs of crustacean and/or eggs of moths provides sufficient nutrients for the mites of the family Parasitidae to grow and/or reproduce.
  • feeding a breeding population of individuals of the mite of the family Parasitidae in a substrate, comprising organic material and vermiculite, with individuals of further mites of the family Carpoglyphidae, Acaridae, Suidasiidae, or a combination thereof, and eggs of crustacean and/or eggs of moths provides a higher density of mites in the substrate compared to a breeding population fed with further mites or eggs of crustacean and/or eggs of moths.
  • Yet another advantage of the combination of individuals of further mites of the family Carpoglyphidae, Acaridae, Suidasiidae, or a combination thereof, and eggs of crustacean and/or eggs of moths is that the nutritional value of individuals of further mites of the family Carpoglyphidae, Acaridae, Suidasiidae and eggs of crustacean and/or eggs of moths complement each other. Furthermore, said further individuals and eggs have dimensions which could be consumed by the individuals of the breeding population.
  • all stadia of the individuals of the further mites may be used for the cultivation of the mites of the family Parasitidae.
  • the different stadia may be eggs of the further mites, larvae of the further mites, nymphs of the further mites, adults of the further mites.
  • said mites in their different stadia of evolution may be fed to the mite of the family Parasitidae alive or dead.
  • Yet another advantage of the method according to the invention is that the achieved cultivated mite in said substrate efficiently and effectively prevents and/or treats and/or cures fly plague and/or a mite plague and/or nematodes and/or louse and/or aphids.
  • the method may start with a step of providing a substrate comprising organic material and vermiculite. Said step may be followed by a step of adding a breeding population of individuals of the mite of the family Parasitidae to the substrate, and a step of feeding the mite with individuals of further mites of the family Carpoglyphidae, Acaridae, and/or Suidasiidae, and eggs of crustacean and/or eggs of moths.
  • the mites of the family Parasitidae are fed with individuals of the family Carpoglyphidae, Acaridae, and Suidasiidae, and eggs of crustacean and/or eggs of moths.
  • the mite may be of the genus Parasitus.
  • the method according to the invention is in particular effective and efficient for cultivation of the mite of the genus Parasitus. Furthermore, mites of the genus Parasitus may efficiently be used as a predator mite. In addition, after curing an invasion of undesired mites and/or flies with the predator mite of the genus Parasitus, the mites of Parasitus only live for a limited amount of time, for example live for about another two weeks, as the life cycle/circle of life is limited, for example limited to about two weeks, for mites of the genus Parasitus.
  • the mite may be Parasitus consanguineous.
  • the mite may be Rhabdocarpais 3onsanguineous.
  • An advantage of the mite being Parasitus consanguineous, such as Rhabdocarpais consanguineus, is that cultivation of said mites is cost effective. Therefore, the method according to the invention is an improvement and/or alternative for conventional methods.
  • a further advantage of the mite being Parasitus consanguineous, preferably Rhabdocarpais consanguineous, is that cultivation of said mites does not require intensive labour. Therefore, intensive training to use and/or cultivate said mites is reduced and/or unnecessary.
  • the further mites are of the genus Carpoglyphus, Tyrophagus, Suidasia, or a combination thereof.
  • the further mites are Carpoglyphus lactis, Tyrophagus putrescentiae, Suidasia medanensis, or combinations thereof.
  • the organic material may be one or more selected from the group of peat, coconut substrate, potting soil, compost.
  • organic material wherein the organic material is one or more selected from the group of peat, coconut substrate, potting soil, compost enables to provide the mites of the family Parasitidae with a sufficient amount of nutrients such as minerals and the like.
  • the peat comprises black peat.
  • black peat in combination with vermiculite provides an efficient and effective substrate for breeding a population of individuals of the mite of the family Parasitidae.
  • Said substrate provides and maintains efficiently and effectively the desired moisture content, and temperature for said individuals of the breeding population as well as the individuals of further mites of the family Carpoglyphidae, Acaridae, Suidasiidae, and eggs of crustacean and/or eggs of moths.
  • the method comprises feeding of the further mites with fishmeal.
  • fishmeal Preferably, at least 200 mL fishmeal is provided to 5 L of substrate comprising further mites.
  • the substrate comprising further mites is equal or the same as the substrate the breeding population of individuals of the mite of the family Parasitidae is bred in.
  • fishmeal may be provided to the substrate suitable for breeding the further mites before said further mites are provided to said substrate.
  • At least 250 mL of fishmeal is provided to 5 L substrate, more preferably at least 300 mL fishmeal is provided to 5 L substrate.
  • the fishmeal increases the protein content in the further mites, in particular the protein content in the mites of the genus Tyrophagus, for example said mites are Tyrophagus putrescentiae .
  • the diameter of the eggs may be in the range of 0.1 mm to 3 mm, preferably in the range of 0.5 mm to 2 mm, more preferably in the range of 0.5 mm to 1.5 mm.
  • the diameter of the eggs may be in the range of 0.1 mm to 3 mm, preferably in the range of 0.5 mm to 2 mm, more preferably in the range of 0.5 mm to 1.5 mm.
  • the diameter is about 1 mm.
  • the eggs of crustacean are eggs of the family Artemiidae, preferably eggs of the genus Artemia and/or the eggs of moths are eggs of the genus Ephestia.
  • the eggs of crustacean are eggs of the family Artemiidae, preferably eggs of the genus Artemia and/or the eggs of moths are eggs of the genus Ephestia enable to provide the mite of the family Parasitidae with the desired nutrients.
  • the eggs of the genus Artemia comprise preferably a diameter of 0.15 mm to 0.30 mm, preferably 0.20 mm to 0.25 mm.
  • the humidity may be in the range of 40% to 80%, preferably in the range of 50% to 70%, more preferably in the range of 55% to 65%, and/or the temperature may be in the range of 20 °C to 30 °C, preferably in the range of 23 °C to 28 °C, more preferably in the range of 25 °C to 27 °C.
  • the method according to the invention is performed in the abovementioned humidity and/or temperature range.
  • the method according to the invention may comprise measuring and/or controlling of the humidity and/or temperature. Said measuring and/or controlling may be combined, wherein the controlling may occur after a measurement. Furthermore, said measuring and/or controlling may be performed manually, in a semi-autonomous mode, or an autonomous mode.
  • the method further comprises isolating the mite in vermiculite.
  • Isolating the mite in vermiculite enables to efficiently and effectively provide the mite of the family Parasitidae to a consumer. Furthermore, this enables to efficiently and effectively use the mite of the family Parasitidae to prevent and/or treat and/or cure an invasion of undesired mites and/or organisms, such as flies, nematodes, louse, and/or aphids, in, for example, agriculture.
  • Isolating the mite in vermiculite may include the steps of:
  • the vermiculite including the mites of the family Parasitidae may be easily removed from the net and provided to a user and/or used at a place an invasion of undesired mites and/or organisms, such as flies, nematodes, louse, and/or aphids, have to be treated and/or cured.
  • the invention also relates to a mite composition
  • a mite composition comprising the mite of the family Parasitidae and vermiculite.
  • the mite composition provides the same effects and advantages as those described for the method for cultivating a mite according to the invention.
  • the mite may be of the genus Parasitus.
  • the mite may be Parasitus consanguineous, more preferably Rhabdocarpais 6dvent6uineous .
  • the mite composition according to the invention is in particular effective and efficient for preventing and/or treating and/or curing an invasion of undesired mites and/or organisms, such as flies.
  • the mite composition according to the invention efficiently and effectively prevents and/or treats and/or cures an invasion of Lucillia sericata, Calliphora vomitoria, Musca domestica, Stomoxys calcitrans, thrips, spider mite, shore flies (scutella), nematodes, and root aphids.
  • said mites of the genus Parasitus may efficiently be used as a predator mite.
  • the mites of Parasitus after curing an invasion of undesired mites and/or flies with the predator mite of the genus Parasitus, the mites of Parasitus only live for about another two weeks as the life cycle/circle of life is about two weeks for mites of the genus Parasitus.
  • An6dventage of the mite being Parasitus consanguineous is that cultivation of said mites is cost effective. Therefore, the mite composition according to the invention is an improvement and/or alternative for conventional mite compositions.
  • a further advantage of the mite being Parasitus consanguineous, such as Rhabdocarpais 6onsanguineous, is that cultivation of said mites does not require intensive labour. Therefore, intensive training to use and/or cultivate said mites is reduced and/or unnecessary.
  • the mite composition further comprises a food substrate, wherein the food substrate may be one or more selected from the group of mites of Carpoglyphus lactis, Tyrophagus putrescentiae, Suidasia medanensis, eggs of crustacean, eggs of moths.
  • the eggs of crustacean are eggs of the family Artemiidae, preferably eggs of the genus Artemia and/or the eggs of moths are eggs of the genus Ephestia.
  • the invention also relates to a system for releasing beneficial mites, comprising:
  • - mites which are arranged in the housing and which can come out of the opening in order to prevent, reduce, and/or treat a fly plague and/or a mite plague and/or nematode and/or louse and/or aphids.
  • the system for releasing beneficial mites provides the same effects and advantages as those described for the method for cultivating a mite according to the invention, and mite composition according to the invention.
  • the mites can deposit their eggs and develop therein.
  • the housing is provided with an opening. It is possible with the system according to the invention, wherein an opening is provided in the housing, to introduce mites into for instance a house or coop or dung lump or barn or stable. The mites introduced into the house, green house, coop, dung lump, barn, stable in this way will leave the system according to the invention through the opening and spread around the desired area such as the house, green house, coop, dung lump, barn, stable.
  • the invention also relates to a use of the mite of the family Parasitidae to prevent, reduce, and/or treat a fly plague and/or a mite plague and/or a nematode plague and/or a louse plague and/or an aphid’s plague.
  • the use of the mite of the family Parasitidae to prevent, reduce, and/or treat a fly plague and/or a mite plague and/or a nematode plague and/or a louse plague and/or an aphid’s plague provides the same effects and advantages as those described for the method for cultivating a mite according to the invention, mite composition according to the invention, and system for releasing beneficial mites according to the invention.
  • the mite may be provided in an amount in the range of 1000 mites to 3000 mites per square metre, preferably in the range of 1500 mites to 2500 mites per square metre.
  • the mites may be provided in multiple amounts, wherein the amounts are provided at least once a week.
  • the mite may be provided in an amount in the range of 100 mites to 300 mites per square metre, preferably in the range of 150 mites to 250 mites per square metre.
  • the mites may be provided in multiple amounts, wherein the amounts are provided at least once a week.
  • the size of the population of mites of the family Parasitidae may be determined using the following method.
  • the substrate comprising the mite of the family Parasitidae is mixed, such that a homogeneous mixture of substrate and mites is achieved. 0.25 millilitre is taken from the substrate using a calibrated spoon. The mites in the content removed with said spoon is then counted using a microscope. All living mites are counted.
  • the total amount of counted mites is multiplied by 500 to provide the amount of mites per Litre of substrate.
  • Parasitus consanguineous for example Rhabdocarpais consanguineus
  • Rhabdocarpais consanguineus were cultivated using a substrate of moist black peat mixed with vermiculite.
  • the ratio between the dry weight of black peat and the dry weight of vermiculite was 1 : 5.
  • the initial colony of about 1000 mites were extensively fed with individuals of further mites of the family Carpoglyphidae, Acaridae, Suidasiidae, or a combination thereof, and eggs of crustacean and/or eggs of moths.
  • the Parasitus consanguineous were fed with Carpoglyphus lactis, Tyrophagus putrescentiae, and/or Suidasia medanensis, and eggs of the genus Artemia.
  • the Parasitus consanguineous were fed with Carpoglyphus lactis, Tyrophagus putrescentiae, and/or Suidasia medanensis, and eggs of the genus Artemia, and eggs of the genus Ephestia.
  • the substrate comprising the initial colony has been kept in an environment with a humidity of about 60%, and a temperature of about 26 °C.
  • a day/night rhythm comprising 16 hours of light, preferably 16 hours of consecutive light, was provided to the substrate comprising the initial colony for 10 days to 14 days.
  • the substrate comprises between 45000 and 55000 mites of Parasitus consanguineous per 5 Litre.
  • two petri dishes were provided with about 20 mites of Parasitus consanguineous.
  • two maggots of the fly also referred to as larvae of the fly, Pucillia sericata were provided to each dish.
  • the dishes were sealed such that flies and/or mites could not escape, but that the humidity and temperature could be controlled such that optimal developing conditions for the maggots of the fly Pucillia sericata were achieved.
  • After four days the maggots transformed into pupas. Said pupas, also referred to as pupea, did not develop into flies. It is proposed that the maggots of the fly Pucillia sericata may not fully develop due to the presence of said mites. When said dishes were opened after six days, the mites were still alive.
  • a first dung lump was formed by the feces of chicken and a second a dung lump was formed by the feces of goats. Both dung lump were invaded by flies, such as flies from the family Sphaeroceridae. After six weeks both dung lumps were cleared from said flies. A sample of both dung lumps showed that Parasitus consanguineous was still present in both dung lumps.
  • the mite composition according to the invention provides an efficient and effective cure of an invasion of flies.
  • a first petri dish is provided with 30 millilitre substrate of peat and vermiculite comprising about 50000 Parasitus consanguineous per 5 Litre substrate.
  • the second petri disch was provided with the same substrate without any predator mite.
  • a chicken coop (1 metre by 2 metres) invaded by the mite Dermanyssus gallinae was cleared by the mite Parasitus consanguineous.
  • the mite Parasitus consanguineous was provided to the chicken coop using four systems for releasing beneficial mites according to the invention. All systems comprises about 1000 mites of Parasitus consanguineous. After four weeks no mites of Dermanyssus gallinae were found in the chicken coop.
  • Parasitus consanguineous (for example Rhabdocarpais consanguineus) were cultivated using a substrate of moist black peat mixed with vermiculite.
  • the ratio between the dry weight of black peat and the dry weight of vermiculite was 1 : 5.
  • To 5 L of the substrate 300 mL of fishmeal was added.
  • 5000 further mites of Tyrophagus putrescentiae were added to the substrate and the substrate has been kept in an environment with a humidity of about 60%, and a temperature of about 26 °C for 24 hours.
  • the fishmeal may enable to achieve Tyrophagus putrescentiae with an increased uptake of proteins.
  • Parasitus consanguineous for example Rhabdocarpais consanguineus
  • Carpoglyphus lactis for example Tyrophagus putrescentiae
  • Suidasia medanensis eggs of the genus Artemia.
  • Parasitus consanguineous for example Rhabdocarpais consanguineus
  • Carpoglyphus lactis for example Tyrophagus putrescentiae
  • Suidasia medanensis eggs of the genus Ephestia.
  • the substrate comprising the initial colony has been kept in an environment with a humidity of about 60%, and a temperature of about 26 °C.
  • a day/night rhythm comprising 16 hours of light, preferably 16 hours of consecutive light, was provided to the substrate comprising the initial colony for 10 days to 14 days. It was found that at about 12 days the substrate comprises between 58000 and 68000 mites of Parasitus consanguineous per 5 Litre of substrate.
  • the mites were about 5% bigger compared to mites fed with Tyrophagus putrescentiae which had not been exposed to fishmeal. The size of the mites was determined using a microscope and a ruler.
  • Parasitus consanguineous may be referred to as P. consanguineous.
  • the amount of the substrate is defined as the total amount of said substrate.
  • a combination of two parts forming the substrate may be defined as 50% of substrate A and 50% of substrate B.
  • Table 1 Composition of substrate.
  • the density of the substrate of entry 1 was too high.
  • the mites were only found in the top part of the substrate. As a result, the full potential of the substrate was not used.
  • the substrate of entry 1 had to be sprayed with an aqueous solution more often compared to the substrate of for example entries 7 and 8 in order to prevent that the substrate dried out.
  • a substrate according to entry 1 is not preferred for cultivating a mite such as P. consanguineous.
  • a substrate according to entry 2 enables a mite such as P. consanguineous to move freely through the substrate. It was also found that said substrate had to be sprayed with an aqueous solution to prevent that the substrate dries out.
  • an additional substrate for example vermiculite such as disclosed in entry 9, could be added such that moist is retained by the substrate. It was found that a substrate of bark and vermiculite in an amount/ratio of 50% : 50% solved the problem of (continuously) wetting the substrate. The addition of vermiculite enabled to achieve a substrate with the desired moisture content.
  • a disadvantage of the bark (entries 2 and 9) was that a large volume is required to grow the desired population. Furthermore, the substrate comprising bark could not efficiently and effectively blow onto a surface which needed to be treated.
  • a substrate of 50% potting soil and 50% compost (entry 4) provided too much heat. Therefore, the mites suffered from stress and eventually died. The desired population of mites could not be achieved. A similar effect was observed using the substrate according to entry 10.
  • a substrate of coconut appeared to have a structure which could not be efficiently and effectively be handled by a user.
  • a substrate of coconut did not provide the desired concentration of at least 50000 P. consanguineous per 5 Litre substrate efficiently and effectively. Therefore, a substrate of coconut is not desired.
  • the substrate according to entry 6 did not provide the desired concentration of at least 50000 P. consanguineous per 5 Litre substrate. Said substrate is not resistant to moisture, therefore said substrate was too dry for the mites.
  • the substrate according to entry 7 provided an efficient and effective population of at least 50000 P. consanguineous per 5 Litre substrate, it was found that a substrate according to entry 8 provided the desired population of at least 50000 P. consanguineous per 5 Litre substrate even more efficiently and effectively compared to the substrate of entry 7 (shorter period of time and less water to be used).
  • a substrate comprising organic material and vermiculite provided an efficient and effective population of at least 50000 P. consanguineous per 5 Litre substrate.
  • Table 2 Composition of diet.

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Insects & Arthropods (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne un procédé de culture d'un acarien, une composition d'acarien comprenant ledit acarien, un système de libération dudit acarien et une utilisation dudit acarien. Le procédé de culture d'un acarien consiste à nourrir une population reproductrice de l'acarien de la famille des Parasitidae dans un substrat, comprenant un matériau organique et de la vermiculite, avec d'autres acariens de la famille des Carpoglyphidae, des Acaridae, des Suidasiidae, ou une combinaison correspondante, et des œufs de crustacés et/ou des œufs de mites.
PCT/NL2023/050465 2022-09-12 2023-09-12 Procédé de culture d'un acarien, composition comprenant ledit acarien, système de libération dudit acarien et utilisation dudit acarien WO2024058656A1 (fr)

Applications Claiming Priority (2)

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NL2033005A NL2033005B1 (en) 2022-09-12 2022-09-12 Method for cultivating a mite, composition comprising said mite, system for releasing said mite, and use of said mite
NL2033005 2022-09-12

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WO2024058656A1 true WO2024058656A1 (fr) 2024-03-21

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2612551A1 (fr) * 2012-01-04 2013-07-10 Koppert B.V. Composition d'acariens comprenant des acariens prédateurs et proie immobilisée mise en contact avec un agent réducteur de champignon et procédés et utilisations associés à l'utilisation de ladite composition
CN103563855A (zh) * 2013-05-20 2014-02-12 中国农业科学院植物保护研究所 一种饲养猎物螨和捕食螨的新方法
WO2019017776A1 (fr) * 2017-07-16 2019-01-24 Koppert B.V. Système pour libérer des acariens utiles et ses utilisations
WO2022185303A1 (fr) * 2021-03-01 2022-09-09 Bio-Bee Sde Eliyahu Ltd Source d'aliment pour agents de lutte biologique et procédés associés

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2612551A1 (fr) * 2012-01-04 2013-07-10 Koppert B.V. Composition d'acariens comprenant des acariens prédateurs et proie immobilisée mise en contact avec un agent réducteur de champignon et procédés et utilisations associés à l'utilisation de ladite composition
CN103563855A (zh) * 2013-05-20 2014-02-12 中国农业科学院植物保护研究所 一种饲养猎物螨和捕食螨的新方法
WO2019017776A1 (fr) * 2017-07-16 2019-01-24 Koppert B.V. Système pour libérer des acariens utiles et ses utilisations
WO2022185303A1 (fr) * 2021-03-01 2022-09-09 Bio-Bee Sde Eliyahu Ltd Source d'aliment pour agents de lutte biologique et procédés associés

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