WO2024055714A1 - 全血压积测量方法 - Google Patents

全血压积测量方法 Download PDF

Info

Publication number
WO2024055714A1
WO2024055714A1 PCT/CN2023/105029 CN2023105029W WO2024055714A1 WO 2024055714 A1 WO2024055714 A1 WO 2024055714A1 CN 2023105029 W CN2023105029 W CN 2023105029W WO 2024055714 A1 WO2024055714 A1 WO 2024055714A1
Authority
WO
WIPO (PCT)
Prior art keywords
liquid
measuring
blood pressure
light intensity
measured
Prior art date
Application number
PCT/CN2023/105029
Other languages
English (en)
French (fr)
Inventor
刘昱东
王光亮
Original Assignee
深圳市卓润生物科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 深圳市卓润生物科技有限公司 filed Critical 深圳市卓润生物科技有限公司
Publication of WO2024055714A1 publication Critical patent/WO2024055714A1/zh

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry

Definitions

  • the invention relates to the technical field of medical detection, and in particular to a method for measuring total blood pressure volume.
  • HCT hematocrit
  • hematocrit means hematocrit, which refers to the proportion of red blood cells in a certain volume of whole blood sample.
  • Traditional chemiluminescence instruments rely on other means to measure HCT of whole blood samples outside the instrument. The Wen's method is usually used for HCT measurement. However, the Wen's method is complex to operate, takes a long time to measure, and requires manual input of the measured results. Chemiluminescence instruments are used to calibrate project test results, and the measurement operations are complex and inefficient. Some chemiluminescence instrument manufacturers integrate a hematocrit module into the instrument to detect the hematocrit of whole blood samples through impedance measurement.
  • the impedance measurement method there are many small gaps in the measurement container, which leads to an increase in repeated measurements.
  • the container is difficult to clean.
  • the detection electrode needs to be in contact with the whole blood sample. Long-term use may easily cause corrosion and aging of the detection electrode.
  • An embodiment of the present application provides a method for measuring total blood pressure volume and a device for measuring total blood pressure volume.
  • the whole blood pressure volume measurement method has no small gaps in the measuring container, which can reduce the difficulty of cleaning the measuring container, avoid corrosion and aging of the detection device, and realize the pressure volume measurement of whole blood samples.
  • one embodiment of the present application uses a double Measurement by wavelength can effectively eliminate the influence of interfering substances in the sample and improve the accuracy of measurement.
  • a method for measuring total blood pressure volume including the following steps:
  • the absorbance A is calculated through the light intensity data D11, light intensity data D12, light intensity data D21 and light intensity data D22.
  • the formula for calculating the absorbance A is:
  • K1 is the conversion coefficient of the measuring container, and Ddark is the dark current reading of the detection system;
  • the hematocrit is obtained from the relationship curve based on the absorbance A.
  • the method before injecting the first liquid to be measured into the measuring container, the method further includes the following steps: injecting a cleaning solution into the measuring container to clean the measuring container.
  • the first direction and the second direction are two directions perpendicular to each other on the horizontal plane.
  • the preparation of the relationship curve includes the following steps:
  • K2 and b are obtained by measuring the absorbance of 10%, 20%, 40%, 60%, and 80% packed volume samples respectively to obtain the calibration curve of absorbance and packed volume, and then fitting the exponential curve. parameter.
  • the measuring container is a cuvette.
  • the optical path length of the measuring container is 4 mm, and K1 is 2.5.
  • a liquid injection mechanism when the first liquid to be measured is injected into the measuring container and when the second liquid to be measured is injected into the measuring container, a liquid injection mechanism is used.
  • Another embodiment of the present application also provides a device for measuring whole blood pressure volume.
  • the measuring container of the whole blood pressure volume measuring device has no small gaps, which can reduce the difficulty of cleaning the measuring container, avoid corrosion and aging of the detection device, and realize the pressure volume measurement of whole blood samples.
  • the whole blood pressure volume measuring device according to an embodiment of the present application can perform dual measurement. Measurement by wavelength can effectively eliminate the influence of interfering substances in the sample and improve the accuracy of measurement.
  • the whole blood pressure measurement device includes a first signal generating end, a first signal receiving end, a second signal generating end, a second signal receiving end and a detector, wherein the first signal generating end and the The first signal receiving end is arranged oppositely, the second signal generating end is arranged oppositely to the second signal receiving end, and the detector is electrically connected to the first signal generating end and the first signal receiving end.
  • the second signal generating end and the second signal receiving end, the first signal generating end and the first signal receiving end can respectively measure the light intensity of the first liquid to be measured from the first direction on the horizontal plane Data D11 and light intensity data D21 of the second liquid to be measured.
  • the second signal generating end and the second signal receiving end can respectively measure the light intensity data D12 and D12 of the first liquid to be measured from the second direction on the horizontal plane.
  • the light intensity data D22 of the second liquid to be measured can be measured.
  • the first direction and the second direction are two directions perpendicular to each other on the horizontal plane.
  • the whole blood pressure volume measurement device further includes a measurement container.
  • the whole blood pressure volume measuring device further includes a liquid filling mechanism, and the liquid filling mechanism is used to fill liquid into the measurement container.
  • the above whole blood pressure volume measurement method has no small gaps in the measurement container, which can reduce the difficulty of cleaning the measurement container, avoid corrosion and aging of the detection device, and realize the pressure volume measurement of whole blood samples.
  • this application uses dual wavelengths of 420nm and 540nm for measurement, which can Effectively eliminate the influence of interfering substances in the sample and improve the accuracy of measurement.
  • Figure 1 is a schematic flow chart of a method for measuring whole blood pressure volume according to some embodiments of the present application
  • Figure 2 is a schematic diagram of a whole blood pressure volume measuring device according to some embodiments of the present application.
  • Figure 3 is a partial schematic diagram of a whole blood pressure volume measuring device according to some embodiments of the present application.
  • first and second are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of indicated technical features. Therefore, features defined as “first” and “second” may explicitly or implicitly include at least one of these features.
  • “plurality” means at least two, such as two, three, etc., unless otherwise expressly and specifically limited.
  • connection In the present invention, unless otherwise clearly stated and limited, the terms “installation”, “connection”, “connection”, “fixing” and other terms should be understood in a broad sense. For example, it can be a fixed connection or a detachable connection. , or integrated into one; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be an internal connection between two elements or an interactive relationship between two elements, unless otherwise specified restrictions. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
  • a first feature being “on” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. touch.
  • the terms “above”, “above” and “above” the first feature is above the second feature may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is higher in level than the second feature.
  • "Below”, “below” and “beneath” the first feature to the second feature may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature has a smaller horizontal height than the second feature.
  • Some embodiments of the present application provide a method for measuring whole blood pressure volume to solve the problems of traditional hematocrit measurement methods such as complicated operation, long measurement time and the need to manually input the measured results into the chemiluminescence instrument when measuring outside the instrument.
  • the efficiency is low, and the impedance measurement method has a large amount of memory in the measurement container 200.
  • the small gaps make it more difficult to clean the measurement container 200 during repeated measurements.
  • the detection electrode needs to be in contact with the whole blood sample, which can easily cause corrosion, aging and hair growth of the detection electrode.
  • Figure 1 is a schematic flow chart of the whole blood pressure volume measurement method according to some embodiments of the present application.
  • the whole blood pressure volume measurement method of the present application The method can be used for whole blood hematocrit measurement purposes.
  • An exemplary method for measuring whole blood pressure volume includes the following steps:
  • Step 1 Inject the first liquid to be measured into the measurement container 200, and measure the light intensity data D11 and light intensity data D12 of the first liquid to be measured from the first direction and the second direction respectively;
  • Step 2 Clean the measurement container 200, inject the second liquid to be measured into the measurement container 200, and measure the light intensity data D21 and light intensity data D22 of the second liquid to be measured from the first direction and the second direction respectively;
  • Step 3 Calculate the absorbance A through the light intensity data D11, light intensity data D12, light intensity data D21 and light intensity data D22.
  • the formula for calculating absorbance A is:
  • K1 is the 200 conversion coefficient of the measuring container, and Ddark is the dark current reading of the detection system;
  • Step 4 Obtain the hematocrit from the relationship curve based on the absorbance A.
  • the following steps are also included: injecting a cleaning solution into the measuring container 200 to clean the measuring container 200 .
  • the first direction and the second direction are two directions perpendicular to each other on the horizontal plane.
  • the preparation of the relationship curve includes the following steps:
  • K2 and b are obtained by measuring the absorbance of 10%, 20%, 40%, 60%, and 80% packed volume samples respectively to obtain the calibration curve of absorbance and packed volume, and then fitting the exponential curve. parameter.
  • the measurement vessel 200 is a cuvette.
  • a liquid injection mechanism is used.
  • FIG. 1 and FIG. 2 are schematic structural diagrams of a whole blood pressure measuring device used in the whole blood pressure measuring method provided by embodiments of the present application.
  • the total blood pressure volume measurement device used in the total blood pressure volume measurement method includes a first signal generating end 101, a first signal receiving end 102, a second signal generating end 103, a second signal receiving end 104, and a detector.
  • the first signal generating end 101 and the first signal receiving end 102 are arranged oppositely
  • the second signal generating end 103 and the second signal receiving end 104 are arranged oppositely
  • the area enclosed between the signal generating end 103 and the second signal receiving end 104 is a detection station.
  • the detector is electrically connected to the first signal generating end 101, the first signal receiving end 102, the second signal generating end 103 and the second signal receiving end 104.
  • the whole blood pressure measurement device further includes a measurement container 200 .
  • the whole blood pressure volume measuring device further includes a liquid filling mechanism, which is used to fill liquid into the measurement container 200 .
  • This embodiment provides a method for measuring whole blood pressure volume.
  • the total blood pressure volume measurement method of this embodiment uses the total blood pressure volume measurement device shown in FIGS. 2 and 3 .
  • the whole blood pressure measurement device includes a first signal generating end 101, a first signal receiving end 102, a second signal generating end 103, a second signal receiving end 104, a measuring container 200, a liquid injection mechanism and a detector, wherein the first The signal generating end 101 is arranged opposite to the first signal receiving end 102, the second signal generating end 103 is arranged opposite to the second signal receiving end 104, the first signal generating end 101, the first signal receiving end 102, the second signal generating end 103 , the area enclosed between the second signal receiving end 104 is the detection station.
  • the detector is electrically connected to the first signal generating end 101, the first signal receiving end 102, the second signal generating end 103 and the second signal receiving end 104.
  • the liquid filling mechanism is used to fill liquid into the measuring container 200 .
  • the whole blood pressure measurement method includes the following steps:
  • Step 1 Inject the cleaning solution into the measuring container 200 through the liquid injection mechanism to clean the measuring container 200 .
  • Step 2 Inject the first liquid to be measured into the measurement container 200 through the liquid injection mechanism, and measure the light intensity data D11 and light intensity data D12 of the first liquid to be measured from the first direction and the second direction that are perpendicular to each other on the horizontal plane. ;
  • Step 3 Clean the measurement container 200, inject the second liquid to be measured into the measurement container 200 through the liquid injection mechanism, and measure the light intensity data D21 of the second liquid to be measured from the first direction and the second direction that are perpendicular to each other on the horizontal plane. and light intensity data D22;
  • Step 4 Calculate the absorbance A through the light intensity data D11, light intensity data D12, light intensity data D21 and light intensity data D22.
  • the formula for calculating absorbance A is:
  • K1 is the conversion coefficient of the measuring container 200.
  • Step 5 Measure the absorbance at different concentrations through the standard solution, and draw a relationship curve based on the relationship between concentration and absorbance.
  • K2 and b are obtained by measuring the absorbance of 10%, 20%, 40%, 60%, and 80% packed volume samples respectively to obtain the calibration curve of absorbance and packed volume, and then fitting the exponential curve. parameter.
  • Step 6 Obtain the hematocrit from the relationship curve based on the absorbance A.
  • This embodiment provides a method for measuring whole blood pressure volume.
  • the total blood pressure volume measurement method of this embodiment uses the total blood pressure volume measurement device shown in FIGS. 2 and 3 .
  • the whole blood pressure measurement device includes a first signal generating end 101, a first signal receiving end 102, a second signal generating end 103, a second signal receiving end 104, a measuring container 200, a liquid injection mechanism and a detector, wherein the first The signal generating end 101 is arranged opposite to the first signal receiving end 102, the second signal generating end 103 is arranged opposite to the second signal receiving end 104, the first signal generating end 101, the first signal receiving end 102, the second signal generating end 103 , the area enclosed between the second signal receiving end 104 is the detection station.
  • the detector is electrically connected to the first signal generating end 101, the first signal receiving end 102, the second signal generating end 103 and the second signal receiving end 104.
  • the liquid filling mechanism is used to fill liquid into the measuring container 200 .
  • the whole blood pressure measurement method includes the following steps:
  • Step 1 Inject the cleaning solution into the measuring container 200 through the liquid injection mechanism to clean the measuring container 200 .
  • Step 2 Inject the first liquid to be measured into the measuring container 200 through the liquid injection mechanism, and inject the first liquid to be measured vertically from the horizontal plane to each other. Measure the light intensity data D11 and light intensity data D12 of the first liquid to be measured in the first direction and the second direction;
  • Step 3 Clean the measurement container 200, inject the second liquid to be measured into the measurement container 200 through the liquid injection mechanism, and measure the light intensity data D21 of the second liquid to be measured from the first direction and the second direction that are perpendicular to each other on the horizontal plane. and light intensity data D22;
  • Step 4 Calculate the absorbance A through the light intensity data D11, light intensity data D12, light intensity data D21 and light intensity data D22.
  • the formula for calculating absorbance A is:
  • K1 is the conversion coefficient of the measuring container 200.
  • Step 5 Measure the absorbance at different concentrations through the standard solution, and draw a relationship curve based on the relationship between concentration and absorbance.
  • K2 and b are obtained by measuring the absorbance of 10%, 20%, 40%, 60%, and 80% packed volume samples respectively to obtain the calibration curve of absorbance and packed volume, and then fitting the exponential curve. parameter.
  • Step 6 Obtain the hematocrit from the relationship curve based on the absorbance A.
  • the above whole blood pressure volume measurement method and the total blood pressure volume measurement method of the present invention have no small gaps in the measurement container 200, which can reduce the difficulty of cleaning the measurement container 200, avoid corrosion and aging of the detection device, and realize the pressure volume measurement of whole blood samples.
  • the present invention selects dual wavelengths of 420nm and 540nm for measurement, which can effectively eliminate the influence of interference substances in the sample and improve the accuracy of measurement.

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

一种全血压积测量方法及装置，方法包括：向测量容器(200)内注入第一待测液体，分别从第一方向、第二方向测量第一待测液体的光强数据D11与光强数据D12；清洗测量容器(200)，向测量容器(200)中注入第二待测液体，分别从第一方向、第二方向测量第二待测液体的光强数据D21和光强数据D22；通过D11、D12、D21及D22计算吸光度A。

Description

全血压积测量方法
本申请要求于2022年09月15日提交中国专利局、申请号为2022111226129、发明名称为“全血压积测量方法”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本发明涉及医疗检测技术领域，具体涉及一种全血压积测量方法。
背景技术
HCT(hematocrit)即红细胞压积，是指一定体积全血样本中红细胞所占的比例。传统的化学发光仪器全血样本的HCT都依靠其他手段在仪器外部进行测量，通常是采用温氏法进行HCT测量，但温氏法操作复杂，测定时间长，而且需要将测得的结果手动输入化学发光仪器对项目测试结果进行校正，测量操作复杂、效率低。部分化学发光仪器厂商采用在仪器中集成压积模块，通过阻抗测量的方式检测全血样本红细胞压积；阻抗测量的方式中，由于测量容器内存在较多细小缝隙，导致增加重复测量时的测量容器的清洗难度，同时检测电极片需要与全血样本接触，长时间使用易造成检测电极片的腐蚀老化。
发明内容
基于此，有必要针对传统的红细胞压积测量方法中存在的在仪器外部进行测量时操作复杂、测定时间长而且需要将测得的结果手动输入化学发光仪器对项目测试结果进行校正、效率低，以及阻抗测量的方式中因测量容器内存在较多细小缝隙导致增加重复测量时的测量容器的清洗难度，同时检测电极片需要与全血样本接触，易造成检测电极片的腐蚀老化发的问题，本申请一实施例提供一种全血压积测量方法以及全血压积测量装置。本申请一实施例的全血压积测量方法测量容器无细小缝隙，能降低测量容器的清洗难度，避免检测器件的腐蚀老化，实现全血样本的压积测量，同时本申请一实施例选用了双波长进行测量，可以有效消除样本中干扰物的影响，提高测量的准确性。
一种全血压积测量方法，包括如下步骤：
向测量容器内注入第一待测液体，分别从第一方向、第二方向上测量所述第一待测液体 的光强数据D11与光强数据D12；
清洗所述测量容器，向所述测量容器中注入第二待测液体，分别从第一方向、第二方向上测量所述第二待测液体的光强数据D21和光强数据D22；
通过光强数据D11、光强数据D12、光强数据D21以及光强数据D22计算得到吸光度A，计算吸光度A的公式为：
其中，K1为测量容器换算系数，D暗为检测系统暗电流读数；
根据所述吸光度A在关系曲线中获取红细胞压积。
在其中一些实施例中，向测量容器内注入第一待测液体之前，还包括如下步骤：向所述测量容器内注入清洗溶液，对所述测量容器进行清洗。
在其中一些实施例中，第一方向与第二方向为水平面上相互垂直的两个方向。
在其中一些实施例中，所述关系曲线的制备包括如下步骤：
通过标准溶液测量不同浓度时的吸光度，并根据浓度与吸光度的关系绘制得到所述关系曲线，其中，所述关系曲线的公式为：
HCT＝k2×eb×A；
其中，K2、b为通过分别测定10％、20％、40％、60％、80％压积样本的吸光度，得到吸光度与压积的标定曲线后，通过指数曲线拟合后所获得的拟合参数。
在其中一些实施例中，所述测量容器为比色皿。
在其中一些实施例中，测量容器的光程＝4mm，则K1＝2.5。
在其中一些实施例中，向测量容器内注入第一待测液体时以及向所述测量容器中注入第二待测液体时，通过注液机构实现。
本申请另一实施例还提供了一种全血压积测量装置。全血压积测量装置的测量容器无细小缝隙，能降低测量容器的清洗难度，避免检测器件的腐蚀老化，实现全血样本的压积测量，本申请一实施例全血压积测量装置的能够进行双波长进行测量，可以有效消除样本中干扰物的影响，提高测量的准确性。
本申请一实施例的全血压积测量装置，包括第一信号发生端、第一信号接收端、第二信号发生端、第二信号接收端以及检测器，其中所述第一信号发生端与所述第一信号接收端相对设置，所述第二信号发生端与所述第二信号接收端相对设置，所述检测器电性连接于所述第一信号发生端、所述第一信号接收端、所述第二信号发生端与所述第二信号接收端，所述第一信号发生端、所述第一信号接收端能够从水平面上的第一方向分别测量第一待测液体的光强数据D11、第二待测液体的光强数据D21，所述第二信号发生端、所述第二信号接收端能够从水平面上的第二方向分别测量第一待测液体的光强数据D12、、第二待测液体的光强数据D22。
在其中一些实施例中，所述第一方向与所述第二方向为水平面上相互垂直的两个方向。
在其中一些实施例中，所述全血压积测量装置还包括测量容器。
在其中一些实施例中，所述全血压积测量装置还包括注液机构，所述注液机构用于向所述测量容器内注液。
上述全血压积测量方法测量容器无细小缝隙，能降低测量容器的清洗难度，避免检测器件的腐蚀老化，实现全血样本的压积测量，同时本申请选用了420nm和540nm双波长进行测量，可以有效消除样本中干扰物的影响，提高测量的准确性。
附图说明
为了更清楚地说明本申请实施例中的技术方案，下面将对实施例描述中所需要使用的附图作简单的介绍。显而易见地，下面描述中的附图仅仅是本申请的一些实施例，对本领域技术人员来说，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。
为了更完整地理解本申请及其有益效果，下面将结合附图来进行说明。其中，在下面的描述中相同的附图标号表示相同部分。
图1为本申请一些实施例所述的全血压积测量方法流程示意图；
图2为本申请一些实施例所述的全血压积测量装置示意图；
图3为本申请一些实施例所述的全血压积测量装置部分示意图。
附图标记说明
101、第一信号发生端；102、第一信号接收端；103、第二信号发生端；104、第二信
号接收端；200、测量容器。
具体实施方式
为使本发明的上述目的、特征和优点能够更加明显易懂，下面结合附图对本发明的具体实施方式做详细的说明。在下面的描述中阐述了很多具体细节以便于充分理解本发明。但是本发明能够以很多不同于在此描述的其它方式来实施，本领域技术人员可以在不违背本发明内涵的情况下做类似改进，因此本发明不受下面公开的具体实施例的限制。
在本发明的描述中，需要理解的是，术语“中心”、“纵向”、“横向”、“长度”、“宽度”、“厚度”、“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”、“内”、“外”、“顺时针”、“逆时针”、“轴向”、“径向”、“周向”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本发明和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。
此外，术语“第一”、“第二”仅用于描述目的，而不能理解为指示或暗示相对重要性或者隐含指明所指示的技术特征的数量。由此，限定有“第一”、“第二”的特征可以明示或者隐含地包括至少一个该特征。在本发明的描述中，“多个”的含义是至少两个，例如两个，三个等，除非另有明确具体的限定。
在本发明中，除非另有明确的规定和限定，术语“安装”、“相连”、“连接”、“固定”等术语应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或成一体；可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通或两个元件的相互作用关系，除非另有明确的限定。对于本领域的普通技术人员而言，可以根据具体情况理解上述术语在本发明中的具体含义。
在本发明中，除非另有明确的规定和限定，第一特征在第二特征“上”或“下”可以是第一和第二特征直接接触，或第一和第二特征通过中间媒介间接接触。而且，第一特征在第二特征“之上”、“上方”和“上面”可是第一特征在第二特征正上方或斜上方，或仅仅表示第一特征水平高度高于第二特征。第一特征在第二特征“之下”、“下方”和“下面”可以是第一特征在第二特征正下方或斜下方，或仅仅表示第一特征水平高度小于第二特征。
需要说明的是，当元件被称为“固定于”或“设置于”另一个元件，它可以直接在另一个元件上或者也可以存在居中的元件。当一个元件被认为是“连接”另一个元件，它可以是直接连接到另一个元件或者可能同时存在居中元件。本文所使用的术语“垂直的”、“水平的”、“上”、“下”、“左”、“右”以及类似的表述只是为了说明的目的，并不表示是唯一的实施方式。
在本发明的描述中，若干的含义是一个以上，多个的含义是两个以上，大于、小于、超过等理解为不包括本数，以上、以下、以内等理解为包括本数。如果有描述到第一、第二只是用于区分技术特征为目的，而不能理解为指示或暗示相对重要性或者隐含指明所指示的技术特征的数量或者隐含指明所指示的技术特征的先后关系。
除非另有定义，本文所使用的所有的技术和科学术语与属于本发明的技术领域的技术人员通常理解的含义相同。本文中在本发明的说明书中所使用的术语只是为了描述具体的实施例的目的，不是旨在于限制本发明。本文所使用的术语“和/或”包括一个或多个相关的所列项目的任意的和所有的组合。
本申请一些实施例提供一种全血压积测量方法，以解决传统的红细胞压积测量方法中存在的在仪器外部进行测量时操作复杂、测定时间长而且需要将测得的结果手动输入化学发光仪器对项目测试结果进行校正、效率低，以及阻抗测量的方式中因测量容器200内存在较多 细小缝隙导致增加重复测量时的测量容器200的清洗难度，同时检测电极片需要与全血样本接触，易造成检测电极片的腐蚀老化发的问题。以下将结合附图对进行说明。
本申请一些实施例提供的全血压积测量方法，示例性的，请参阅图1所示，图1为本申请一些实施例所述的全血压积测量方法流程示意图，本申请的全血压积测量方法能够用于全血红细胞压积测量用途。
为了更清楚的说明全血压积测量方法的结构，以下将结合附图对全血压积测量方法进行介绍。
示例性的，一种全血压积测量方法，包括如下步骤：
步骤1、向测量容器200内注入第一待测液体，分别从第一方向、第二方向上测量第一待测液体的光强数据D11与光强数据D12；
步骤2、清洗测量容器200，向测量容器200中注入第二待测液体，分别从第一方向、第二方向上测量第二待测液体的光强数据D21和光强数据D22；
步骤3、通过光强数据D11、光强数据D12、光强数据D21以及光强数据D22计算得到吸光度A，计算吸光度A的公式为：
其中，K1为测量容器200换算系数，D暗为检测系统暗电流读数；
步骤4、根据吸光度A在关系曲线中获取红细胞压积。
在其中一些实施例中，向测量容器200内注入第一待测液体之前，还包括如下步骤：向测量容器200内注入清洗溶液，对测量容器200进行清洗。
在其中一些实施例中，第一方向与第二方向为水平面上相互垂直的两个方向。
在其中一些实施例中，关系曲线的制备包括如下步骤：
通过标准溶液测量不同浓度时的吸光度，并根据浓度与吸光度的关系绘制得到关系曲线，其中，关系曲线的公式为：
HCT＝k2×eb×A；
其中，K2、b为通过分别测定10％、20％、40％、60％、80％压积样本的吸光度，得到吸光度与压积的标定曲线后，通过指数曲线拟合后所获得的拟合参数。
在其中一些实施例中，测量容器200为比色皿。
在其中一些实施例中，测量容器200的光程＝4mm，则K1＝2.5。
在其中一些实施例中，向测量容器200内注入第一待测液体时以及向测量容器200中注入第二待测液体时，通过注液机构实现。
在其中一些实施例中，示例性的，请参阅图1及图2所示，图1、图2为本申请实施例提供的全血压积测量方法使用的全血压积测量装置的结构示意图。
在其中一些实施例中，全血压积测量方法使用的全血压积测量装置包括第一信号发生端101、第一信号接收端102、第二信号发生端103、第二信号接收端104以及检测器，其中第一信号发生端101与第一信号接收端102相对设置，第二信号发生端103与第二信号接收端104相对设置，第一信号发生端101、第一信号接收端102、第二信号发生端103、第二信号接收端104之间围成区域为检测工位。检测器电性连接于第一信号发生端101、第一信号接收端102、第二信号发生端103与第二信号接收端104。
在其中一些实施例中，全血压积测量装置还包括测量容器200。
在其中一些实施例中，全血压积测量装置还包括注液机构，注液机构用于向测量容器200内注液。
实施例1
本实施例提供了一种全血压积测量方法。
本实施例的全血压积测量方法使用图2与图3所示的全血压积测量装置。其中，全血压积测量装置包括第一信号发生端101、第一信号接收端102、第二信号发生端103、第二信号接收端104、测量容器200、注液机构以及检测器，其中第一信号发生端101与第一信号接收端102相对设置，第二信号发生端103与第二信号接收端104相对设置，第一信号发生端101、第一信号接收端102、第二信号发生端103、第二信号接收端104之间围成区域为检测工位。检测器电性连接于第一信号发生端101、第一信号接收端102、第二信号发生端103与第二信号接收端104。注液机构用于向测量容器200内注液。
全血压积测量方法，包括如下步骤：
步骤1、通过注液机构向测量容器200内注入清洗溶液，对测量容器200进行清洗。
步骤2、通过注液机构向测量容器200内注入第一待测液体，分别从水平面上相互垂直的第一方向、第二方向上测量第一待测液体的光强数据D11与光强数据D12；
步骤3、清洗测量容器200，通过注液机构向测量容器200中注入第二待测液体，分别从水平面上相互垂直的第一方向、第二方向上测量第二待测液体的光强数据D21和光强数据D22；
步骤4、通过光强数据D11、光强数据D12、光强数据D21以及光强数据D22计算得到吸光度A，计算吸光度A的公式为：
其中，K1为测量容器200换算系数，测量容器200的光程＝4mm，则K1＝2.5，D暗为检测系统暗电流读数；
步骤5、通过标准溶液测量不同浓度时的吸光度，并根据浓度与吸光度的关系绘制得到关系曲线，其中，关系曲线的公式为：
HCT＝k2×eb×A；
其中，K2、b为通过分别测定10％、20％、40％、60％、80％压积样本的吸光度，得到吸光度与压积的标定曲线后，通过指数曲线拟合后所获得的拟合参数。
步骤6、根据吸光度A在关系曲线中获取红细胞压积。
实施例2
本实施例提供了一种全血压积测量方法。
本实施例的全血压积测量方法使用图2与图3所示的全血压积测量装置。其中，全血压积测量装置包括第一信号发生端101、第一信号接收端102、第二信号发生端103、第二信号接收端104、测量容器200、注液机构以及检测器，其中第一信号发生端101与第一信号接收端102相对设置，第二信号发生端103与第二信号接收端104相对设置，第一信号发生端101、第一信号接收端102、第二信号发生端103、第二信号接收端104之间围成区域为检测工位。检测器电性连接于第一信号发生端101、第一信号接收端102、第二信号发生端103与第二信号接收端104。注液机构用于向测量容器200内注液。
全血压积测量方法，包括如下步骤：
步骤1、通过注液机构向测量容器200内注入清洗溶液，对测量容器200进行清洗。
步骤2、通过注液机构向测量容器200内注入第一待测液体，分别从水平面上相互垂直 的第一方向、第二方向上测量第一待测液体的光强数据D11与光强数据D12；
步骤3、清洗测量容器200，通过注液机构向测量容器200中注入第二待测液体，分别从水平面上相互垂直的第一方向、第二方向上测量第二待测液体的光强数据D21和光强数据D22；
步骤4、通过光强数据D11、光强数据D12、光强数据D21以及光强数据D22计算得到吸光度A，计算吸光度A的公式为：
其中，K1为测量容器200换算系数，测量容器200的光程＝4mm，则K1＝2.5，D暗为检测系统暗电流读数；
步骤5、通过标准溶液测量不同浓度时的吸光度，并根据浓度与吸光度的关系绘制得到关系曲线，其中，关系曲线的公式为：
HCT＝k2×eb×A；
其中，K2、b为通过分别测定10％、20％、40％、60％、80％压积样本的吸光度，得到吸光度与压积的标定曲线后，通过指数曲线拟合后所获得的拟合参数。
步骤6、根据吸光度A在关系曲线中获取红细胞压积。
通过本实施例测量20份全血样本后，测量数据见表1、2所示。
表1

表2

由表1与表2数据可知，本实施例测量20份全血样本，每份样本均测量3次，3次数据均有效。
综上，上述全血压积测量方法，本发明的全血压积测量方法测量容器200无细小缝隙，能降低测量容器200的清洗难度，避免检测器件的腐蚀老化，实现全血样本的压积测量，同时本发明选用了420nm和540nm双波长进行测量，可以有效消除样本中干扰物的影响，提高测量的准确性。
在上述实施例中，对各个实施例的描述都各有侧重，某个实施例中没有详述的部分，可以参见其他实施例的相关描述。
以上所述实施例的各技术特征可以进行任意的组合，为使描述简洁，未对上述实施例中的各个技术特征所有可能的组合都进行描述，然而，只要这些技术特征的组合不存在矛盾，都应当认为是本说明书记载的范围。
以上所述实施例仅表达了本发明的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对本发明专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进，这些都属于本发明的保护范围。因此，本发明专利的保护范围应以所附权利要求为准。

Claims (11)

  1. 一种全血压积测量方法，包括如下步骤：
    向测量容器内注入第一待测液体；
    分别从第一方向、第二方向上测量所述第一待测液体的光强数据D11与光强数据D12；
    清洗所述测量容器，向所述测量容器中注入第二待测液体；
    分别从第一方向、第二方向上测量所述第二待测液体的光强数据D21和光强数据D22；
    通过光强数据D11、光强数据D12、光强数据D21以及光强数据D22计算得到吸光度A，计算吸光度A的公式为：
    其中，K1为测量容器换算系数，D暗为检测系统暗电流读数；
    根据所述吸光度A在关系曲线中获取红细胞压积。
  2. 根据权利要求1所述的全血压积测量方法，其特征在于，向测量容器内注入第一待测液体之前，还包括如下步骤：向所述测量容器内注入清洗溶液，对所述测量容器进行清洗。
  3. 根据权利要求1所述的全血压积测量方法，其特征在于，所述第一方向与所述第二方向为水平面上相互垂直的两个方向。
  4. 根据权利要求1～3任意一项所述的全血压积测量方法，其特征在于，所述关系曲线的制备包括如下步骤：
    通过标准溶液测量不同浓度时的吸光度，并根据浓度与吸光度的关系绘制得到所述关系曲线，其中，所述关系曲线的公式为：
    HCT＝k2×eb×A；
    其中，K2、b为通过分别测定10％、20％、40％、60％、80％压积样本的吸光度，得到吸光度与压积的标定曲线后，通过指数曲线拟合后所获得的拟合参数。
  5. 根据权利要求1～3任意一项所述的全血压积测量方法，其特征在于，所述测量容器为比色皿。
  6. 根据权利要求1～3任意一项所述的全血压积测量方法，其特征在于，测量容器的光程＝4mm，则K1＝2.5。
  7. 根据权利要求1～3任意一项所述的全血压积测量方法，其特征在于，向测量容器内注入第一待测液体时以及向所述测量容器中注入第二待测液体时，通过注液机构实现。
  8. 一种全血压积测量装置，所述全血压积测量装置包括第一信号发生端、第一信号接收端、第二信号发生端、第二信号接收端以及检测器，其中所述第一信号发生端与所述第一信号接收端相对设置，所述第二信号发生端与所述第二信号接收端相对设置，所述检测器电性连接于所述第一信号发生端、所述第一信号接收端、所述第二信号发生端与所述第二信号接收端，所述第一信号发生端、所述第一信号接收端能够从水平面上的第一方向分别测量第一待测液体的光强数据D11、第二待测液体的光强数据D21，所述第二信号发生端、所述第二信号接收端能够从水平面上的第二方向分别测量第一待测液体的光强数据D12、、第二待测液体的光强数据D22。
  9. 根据权利要求8所述的全血压积测量装置，其特征在于，所述第一方向与所述第二方向为水平面上相互垂直的两个方向。
  10. 根据权利要求8或9所述的全血压积测量装置，其特征在于，所述全血压积测量装置还包括测量容器。
  11. 根据权利要求10所述的全血压积测量装置，其特征在于，所述全血压积测量装置还 包括注液机构，所述注液机构用于向所述测量容器内注液。
PCT/CN2023/105029 2022-09-15 2023-06-30 全血压积测量方法 WO2024055714A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202211122612.9 2022-09-15
CN202211122612.9A CN115452744A (zh) 2022-09-15 2022-09-15 全血压积测量方法

Publications (1)

Publication Number Publication Date
WO2024055714A1 true WO2024055714A1 (zh) 2024-03-21

Family

ID=84304506

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2023/105029 WO2024055714A1 (zh) 2022-09-15 2023-06-30 全血压积测量方法

Country Status (2)

Country Link
CN (1) CN115452744A (zh)
WO (1) WO2024055714A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115452744A (zh) * 2022-09-15 2022-12-09 深圳市卓润生物科技有限公司 全血压积测量方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160238521A1 (en) * 2013-10-16 2016-08-18 Institut National De La Sante Et De La Recherche Medical Simultaneous monitoring of fibrinogen and haematocrit in whole blood
CN108738338A (zh) * 2016-01-28 2018-11-02 西门子医疗保健诊断公司 用于检测样本中的干扰物的方法和装置
CN113533226A (zh) * 2020-04-13 2021-10-22 深圳迈瑞生物医疗电子股份有限公司 一种抗干扰测量方法及样本分析仪
CN113533244A (zh) * 2021-06-18 2021-10-22 中国科学院深圳先进技术研究院 一种红细胞比容的检测方法、装置、终端和可读存储介质
CN114034653A (zh) * 2021-11-17 2022-02-11 清华大学 基于深度学习的波长调制吸收光谱层析重建系统
CN115452744A (zh) * 2022-09-15 2022-12-09 深圳市卓润生物科技有限公司 全血压积测量方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160238521A1 (en) * 2013-10-16 2016-08-18 Institut National De La Sante Et De La Recherche Medical Simultaneous monitoring of fibrinogen and haematocrit in whole blood
CN108738338A (zh) * 2016-01-28 2018-11-02 西门子医疗保健诊断公司 用于检测样本中的干扰物的方法和装置
CN113533226A (zh) * 2020-04-13 2021-10-22 深圳迈瑞生物医疗电子股份有限公司 一种抗干扰测量方法及样本分析仪
CN113533244A (zh) * 2021-06-18 2021-10-22 中国科学院深圳先进技术研究院 一种红细胞比容的检测方法、装置、终端和可读存储介质
CN114034653A (zh) * 2021-11-17 2022-02-11 清华大学 基于深度学习的波长调制吸收光谱层析重建系统
CN115452744A (zh) * 2022-09-15 2022-12-09 深圳市卓润生物科技有限公司 全血压积测量方法

Also Published As

Publication number Publication date
CN115452744A (zh) 2022-12-09

Similar Documents

Publication Publication Date Title
WO2024055714A1 (zh) 全血压积测量方法
US20150068924A1 (en) Blood glucose measurement module, smart phone combinable with the blood glucose measurement module, and blood glucose measurement method using the same
CN103983669A (zh) 检测试片、检测装置及检测方法
CN106018773A (zh) 一次性血液细胞计数及分析集成试剂传感件及新型血液分析仪
WO2024046369A1 (zh) 一种抗干扰的水质分析仪器测试方法
CN105628747B (zh) 凝血时间测试分析装置及其测试分析方法
CN105572397A (zh) 凝血时间测试分析方法
Burgos-Flórez et al. TBISTAT: An open-source, wireless portable, electrochemical impedance spectroscopy capable potentiostat for the point-of-care detection of S100B in plasma samples
CN112816425B (zh) 一种利用hgb校准能力优化全血样本检测流程的方法
CN111912974B (zh) 一种免疫试剂定标方法
CN115901885B (zh) 代谢指标检测方法、系统及电化学测量系统
CN202886316U (zh) 一种抽测谷物含水率的装置
CN205826667U (zh) 一次性血液细胞计数及分析集成件以及新型血液分析仪
CN215339820U (zh) 一种溶解氧表校准测试系统
CN205333651U (zh) 一种血液中葡萄糖含量的检测装置
CN110514715B (zh) 一种基于i2c通信的血指标检测系统
CN201247216Y (zh) 泡沫携液能力测量装置
CN210005590U (zh) 一种正反向微波功率测量电路
CN108680623B (zh) 一种便携式电化学指纹图谱测定仪
CN103162770B (zh) 用于免疫组化染色机的微量试剂液面检测方法及装置
TWI481869B (zh) 血球容積比測量方法、血糖濃度測量方法及使用前述方法的電化學量測裝置
CN112305245B (zh) 样本分析方法及样本分析系统
CN112379063B (zh) 一种同时含有酒精及其他物质的酒类的酒精浓度测试方法
CN211785103U (zh) 光度计用于进行微量测试的比色盘组合
CN117990768B (zh) 一种体液测定方法、装置和电子设备

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 23864451

Country of ref document: EP

Kind code of ref document: A1