WO2024021269A1 - 一种冷冻电镜用冷台及其控制系统 - Google Patents

一种冷冻电镜用冷台及其控制系统 Download PDF

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Publication number
WO2024021269A1
WO2024021269A1 PCT/CN2022/120458 CN2022120458W WO2024021269A1 WO 2024021269 A1 WO2024021269 A1 WO 2024021269A1 CN 2022120458 W CN2022120458 W CN 2022120458W WO 2024021269 A1 WO2024021269 A1 WO 2024021269A1
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Prior art keywords
cold
cryo
fixedly connected
plate
bottom plate
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PCT/CN2022/120458
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English (en)
French (fr)
Inventor
刘智
李晓润
毛静妍
刘宇
方威
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维亚生物科技(上海)有限公司
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Publication of WO2024021269A1 publication Critical patent/WO2024021269A1/zh

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    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/02—Details
    • H01J37/20—Means for supporting or positioning the object or the material; Means for adjusting diaphragms or lenses associated with the support
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
    • G01N23/2204—Specimen supports therefor; Sample conveying means therefore
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26—Electron or ion microscopes; Electron or ion diffraction tubes
    • H01J37/261—Details
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26—Electron or ion microscopes; Electron or ion diffraction tubes
    • H01J37/261—Details
    • H01J37/265—Controlling the tube; circuit arrangements adapted to a particular application not otherwise provided, e.g. bright-field-dark-field illumination

Definitions

  • the invention relates to the technical field of electron microscopy equipment, and in particular to a cold stage for cryo-electron microscopy and its control system.
  • cryo-electron microscopy is a device used for real-time observation of biological and polymer materials that are sensitive to electron beams.
  • the cold source of cryo-electron microscopy basically comes from liquid helium or liquid nitrogen, but after a certain period of time, liquid nitrogen or liquid helium will appear. If it is exhausted, the refrigerant needs to be refilled, resulting in short working time.
  • a cold head is used to provide a cold source to reach the refrigeration temperature of liquid helium or liquid nitrogen, which is easy to use and has a long working time.
  • the purpose of the present invention is to provide a cold stage for cryo-electron microscopy and its control system, aiming to solve the problem in the prior art that the sample stage is placed below the cryo-electron microscope body and is partially blocked by the cryo-electron microscope body, resulting in the placement of observed substances. Sometimes it will be hindered and the observation object cannot be placed conveniently due to technical problems.
  • the present invention adopts a cryo-electron microscope cold stage, which includes a cryo-electron microscope body and a placement mechanism.
  • the placement mechanism includes a base plate, a flange ring, an adjustment unit, a guide rail, a slider and a sample stage.
  • the cryo-electron microscope body is fixedly connected to the bottom plate and located above the bottom plate
  • the flange ring is fixedly connected to the bottom plate and located below the bottom plate
  • the guide rail is fixedly connected to the bottom plate
  • the number of the adjustment units is two.
  • the two adjustment units are respectively arranged above the bottom plate, and the output end of each adjustment unit passes through the guide rail respectively.
  • the slide block is slidingly connected to the guide rail and is located inside the guide rail.
  • the sample stage is fixedly connected to the slide block and is located above the slide block respectively.
  • each adjustment unit includes an electric cylinder and an electromagnetic assembly.
  • the electric cylinder is fixedly connected to the base plate and is located above the base plate.
  • the electromagnetic assembly is fixedly connected to the output end of the electric cylinder, and They are respectively located at one end of the output end of the electric cylinder, and the electromagnetic assembly penetrates the guide rail.
  • each of the electromagnetic components includes a main rod body, a energized coil and an outer cylinder.
  • the main rod body is fixedly connected to the output end of the electric cylinder and is located at one end of the output end of the electric cylinder, and the main rod body passes through
  • the guide rail the energized coil is fixedly connected to the main rod body, and is sleeved on the outer wall of the main rod body
  • the outer cylinder is fixedly connected to the main rod body, and is sleeved on the outer surface of the main rod body. wall, and the energized coil is located between the main rod body and the outer cylinder.
  • the sample stage includes a plate body, a cold guide plate, a connecting sleeve and an outer ring.
  • the plate body is fixedly connected to the slider and is located above the slider.
  • the cold guide plate is connected to the slider.
  • the plate body is fixedly connected and located above the plate body.
  • the connecting sleeve is fixedly connected to the cold conductive plate and located on the outer wall of the cold conductive plate.
  • the outer ring is fixed to the cold conductive plate. connected and located above the cold plate.
  • the sample stage further includes a cold-conducting glue, and the cold-conducting glue is evenly laid on the inner surface wall of the connecting sleeve.
  • cryo-electron microscope cold stage further includes a cooling mechanism, and the cooling mechanism is arranged above the bottom plate.
  • the cooling mechanism includes a frame body, a cooling tube, a contact plate, a shell and an insulation material.
  • the frame body is fixedly connected to the bottom plate and is located above the bottom plate.
  • the shell is connected to the bottom plate.
  • the frame body is fixedly connected and located above the frame body.
  • the cooling pipe is arranged inside the frame body, and the insulation material is filled between the cooling pipe and the frame body.
  • the contact The plate is fixedly connected to the cooling pipe and located at one end of the cooling pipe.
  • the cooling mechanism further includes an inner transfer pipe, the inner transfer pipe is arranged inside the cold transfer pipe, and one end of the inner transfer pipe is provided with a rounded corner.
  • the invention also provides a control system, including a refrigeration switch, a temperature adjustment knob and a drive switch.
  • the refrigeration switch is arranged above the bottom plate, the temperature adjustment knob is arranged above the bottom plate, and the drive switch is arranged Above the base plate, the drive switch is electrically connected to each of the electromagnetic coils and the electric cylinder.
  • an observation sample is placed on the sample stage, and one of the adjustment units is activated to adjust the slider, so that the slider is positioned at the desired position.
  • the above structure allows the sample stage to be moved to facilitate the placement or replacement of the observation material, so that the material to be placed for observation is located at the best position. status and location.
  • Figure 1 is a schematic structural diagram of the first embodiment of the present invention.
  • Figure 2 is a top view of the first embodiment of the present invention.
  • Figure 3 is a front view of the first embodiment of the present invention.
  • Fig. 4 is a structural cross-sectional view along line A-A of Fig. 2 of the present invention.
  • Fig. 5 is an enlarged view of the partial structure at B of Fig. 4 of the present invention.
  • Figure 6 is a front view of the second embodiment of the present invention.
  • Fig. 7 is a structural cross-sectional view along line C-C of Fig. 6 of the present invention.
  • Figure 8 is a schematic structural diagram of the third embodiment of the present invention.
  • FIG. 1 is a schematic structural diagram of the first embodiment
  • FIG. 2 is a top view of the first embodiment
  • FIG. 3 is a front view of the first embodiment
  • FIG. 4 is the A-A line structure of FIG. 2 Cross-sectional view
  • Figure 5 is an enlarged view of the partial structure at B in Figure 4.
  • the present invention provides a cold stage for cryo-electron microscopy: it includes a cryo-electron microscope body 101 and a placement mechanism.
  • the placement mechanism includes a base plate 102, a flange ring 103, Adjustment unit, guide rail 104, slider 105 and sample stage.
  • the adjustment unit includes an electric cylinder 106 and an electromagnetic assembly.
  • the electromagnetic assembly includes a main rod body 107, a energized coil 108 and an outer cylinder 109.
  • the sample stage includes a plate body 110 , cold guide plate 111, connecting sleeve 112, outer ring 113 and cold guide glue 114.
  • the electric cylinder 106 is used to drive the electromagnetic component to move, and the cooperation between the electric cylinder 106 and the electromagnetic component is used to move the slider 105 and cause the sample stage to move.
  • the cold-conducting plate 111 and the connecting sleeve 112 can conduct low temperature, thereby freezing the observation sample.
  • the cryo-EM body 101 is fixedly connected to the bottom plate 102 and is located above the bottom plate 102.
  • the flange ring 103 is fixedly connected to the bottom plate 102 and is located below the bottom plate 102.
  • the guide rail 104 is fixedly connected to the bottom plate 102 and is located above the bottom plate 102.
  • the number of the adjustment units is two. The two adjustment units are respectively disposed above the bottom plate 102, and each of the The output end of the adjustment unit passes through the guide rail 104 respectively.
  • the slider 105 is slidingly connected to the guide rail 104 and is located inside the guide rail 104.
  • the sample stage is fixedly connected to the slider 105 and is located respectively.
  • the slider 105 place the observation sample on the sample stage, start one of the adjustment units to adjust the slider 105, and push the slider 105 on the guide rail 104 , until it is pushed to one side of the guide rail 104, and then starts another adjustment component to drive, pushing the slider 105 to one side of the guide rail 104, so that the sample stage is located on the cryo-EM body 101 for observation directly below, and at the same time, the sample stage is connected to the cooling end, where the flange ring 103 is used to install and fix the cold stage for cryo-electron microscopy.
  • the electric cylinder 106 is fixedly connected to the base plate 102 and is located above the base plate 102.
  • the electromagnetic components are fixedly connected to the output end of the electric cylinder 106 and are respectively located at the output ends of the electric cylinder 106.
  • One end of the end, and the electromagnetic component penetrates the guide rail 104.
  • the telescopic drive of the electric cylinder 106 can telescope the electromagnetic component.
  • the electromagnetic component performs electromagnetic magnetic attraction on the slider 105 to achieve the movement adjustment. Describe the purpose of slider 105.
  • the main rod body 107 is fixedly connected to the output end of the electric cylinder 106 and is located at one end of the output end of the electric cylinder 106.
  • the main rod body 107 penetrates the guide rail 104, and the energized coil 108 is connected to the output end of the electric cylinder 106.
  • the main rod body 107 is fixedly connected and sleeved on the outer wall of the main rod body 107.
  • the outer tube 109 is fixedly connected to the main rod body 107 and sleeved on the outer wall of the main rod body 107, and the The energizing coil 108 is located between the main rod body 107 and the outer cylinder 109.
  • the outer cylinder 109 protects the energizing coil 108 and energizes the energizing coil 108 when the slider 105 is moved. , thereby causing the main rod body 107 to have electromagnetic force, so that the main rod body 107 magnetically fixes the slider 105 .
  • the plate body 110 is fixedly connected to the slider 105 and is located above the slider 105
  • the cold-conducting plate 111 is fixedly connected to the plate body 110 and is located above the plate body 110
  • the connecting sleeve 112 is fixedly connected to the cold guide plate 111 and is located on the outer wall of the cold guide plate 111
  • the outer ring 113 is fixedly connected to the cold guide plate 111 and is located on the cold guide plate 111
  • the connecting sleeve 112 is used to connect the cold transfer equipment
  • the plate body 110 supports the cold conducting plate 111
  • the outer ring 113 presses the cold conducting plate 111.
  • a container capable of loading observation objects is formed, in which both the cold-conducting plate 111 and the connecting sleeve 112 are made of copper or silver.
  • the cold-conducting glue 114 is evenly laid on the inner surface wall of the connecting sleeve 112. The laying of the cold-conducting glue 114 can eliminate the connection gap when connecting the cold-conducting equipment and enhance the conduction of low temperature.
  • the observation sample is placed on the sample stage, and one of the adjustment units is started to adjust the slider 105, and the slider 105 is moved to the desired position.
  • the telescopic drive of the electric cylinder 106 can telescope the electromagnetic assembly, and at the same time, the electromagnetic assembly can be telescoped through
  • the electromagnetic assembly performs electromagnetic magnetic attraction on the slider 105 to achieve the purpose of moving and adjusting the slider 105.
  • the outer cylinder 109 protects the energized coil 108 while moving the slider 105.
  • the energizing coil 108 is energized, so that the main rod body 107 has electromagnetic force, so that the main rod body 107 magnetically fixes the slider 105 in the sample stage.
  • connection sleeve 112 is used to connect the cold transfer equipment, the plate body 110 supports the cold guide plate 111, and the outer ring 113 presses the cold guide plate 111, and at the same time forms a loadable A container for observation objects, in which the cold-conducting plate 111 and the connecting sleeve 112 are made of copper or silver.
  • the laying of the cold-conducting glue 114 can eliminate the connection gap when connecting the cold-conducting equipment and enhance the conduction of low temperature. .
  • Figure 6 is a front view of the second embodiment
  • Figure 7 is a structural cross-sectional view of the C-C line in Figure 6.
  • the present invention provides a cold stage for cryo-electron microscopy.
  • a cooling mechanism which includes a frame 201, a cooling pipe 202, a contact plate 203, a shell 204, an insulation material 205 and an internal transfer pipe 206.
  • low temperature is conducted through the contact plate 203, and the housing 204 protects the insulation material 205 and the cold pipe 202.
  • the insulation material 205 can prevent the loss of temperature.
  • the frame body 201 is fixedly connected to the bottom plate 102 and is located above the bottom plate 102.
  • the housing 204 is fixedly connected to the frame body 201 and is located above the frame body 201.
  • the cooling pipe 202 is disposed inside the frame 201 , and the insulation material 205 is filled between the cooling pipe 202 and the frame 201 .
  • the contact plate 203 is fixedly connected to the cooling pipe 202 , and is located at one end of the cold pipe 202.
  • the frame 201 supports the shell 204, and at the same time, the shell 204 wraps the insulation material 205 and the cold pipe 202.
  • the thermal insulation material 205 can prevent the cold pipe 202 from reducing temperature loss when conducting low temperature.
  • the contact plate 203 is used to contact the output end of the refrigeration equipment to achieve the purpose of conducting cold.
  • the inner transfer pipe 206 is arranged inside the cold transfer pipe 202, and one end of the inner transfer pipe 206 is provided with a rounded corner.
  • the inner transfer pipe 206 is made of copper or silver, which improves the cooling efficiency. It is higher and adopts rounded corners, which can better facilitate the introduction of the connecting sleeve 112.
  • the frame 201 supports the housing 204, and at the same time, the housing 204 wraps the thermal insulation material 205 and the cooling tube 202.
  • the insulation material 205 can prevent the cold pipe 202 from reducing temperature loss when conducting low temperature.
  • the contact plate 203 is used to contact the output end of the refrigeration equipment to achieve the purpose of conducting cold.
  • the inner transfer tube 206 is made of copper or silver, which makes the cooling efficiency higher. At the same time, it is arranged with rounded corners, which can better facilitate the introduction of the connecting sleeve 112.
  • the present invention provides a cold stage for cryo-electron microscopy: it also includes a refrigeration mechanism, and the refrigeration mechanism includes a ring frame 301 , cold head 302, collar 303, spring 304, adjustment ring 305 and limiting nail 306.
  • low temperature is produced through the cold head 302 and conducted through the contact plate 203.
  • the cold head 302 is arranged above the contact plate 203 through the ring frame 301, and at the same time passes through the contact plate 203.
  • the adjustment ring 305 adjusts the height of the collar 303, thereby adjusting the height of the cold head 302.
  • the ring frame 301 is fixedly connected to the housing 204 and is sleeved on the outer wall of the housing 204.
  • the cold heads 302 are fixedly connected and are respectively located on the outer wall of the cold head 302, and each of the collars 303 is set on the outer wall of the ring frame 301.
  • There are multiple adjustment rings 305, and each adjustment ring 305 They are threadedly connected to the ring frame 301 and respectively set on the outer wall of the ring frame 301.
  • the cold head 302 produces low temperature and conducts transmission through the contact plate 203.
  • the cold head 302 passes through the
  • the ring frame 301 is arranged above the contact plate 203.
  • the height of the collar 303 is adjusted through the adjustment ring 305 to adjust the height of the cold head 302.
  • the spring 304 The elastic force can make the adjustment ring 305 hold tighter, so that the adjustment ring 305 is less likely to loosen.
  • the number of the limiting nails 306 is multiple.
  • Each of the limiting nails 306 is threadedly connected to the ring frame 301 and is located above the ring frame 301 respectively.
  • the limiting nails 306 can This prevents the adjustment ring 305 from detaching, thus providing effective protection.
  • the cold head 302 When using a cold stage for cryo-electron microscopy in this embodiment, the cold head 302 produces low temperature and conducts transmission through the contact plate 203.
  • the cold head 302 is arranged on the contact plate through the ring frame 301.
  • the height of the collar 303 is adjusted through the adjustment ring 305, thereby adjusting the height of the cold head 302.
  • the elastic force of the spring 304 can make the adjustment ring 305
  • the clamping is tighter, so that the adjustment ring 305 is less likely to loosen, and the limiting nail 306 can prevent the adjustment ring 305 from being detached, thus providing effective protection.
  • the present invention also provides a control system, including a refrigeration switch 401, a temperature adjustment knob 402 and a drive switch 403.
  • the refrigeration switch 401 is arranged above the bottom plate 102, and the temperature adjustment knob 402 is arranged on the bottom of the bottom plate 102.
  • the drive switch 403 is disposed above the base plate 102 , and the drive switch 403 is electrically connected to each of the electromagnetic coils and the electric cylinder 106 .
  • the refrigeration switch 401 controls the cold head 302, opens and closes the cold head 302, and at the same time makes the cold head 302 produce the required stability through the temperature adjustment knob 402.
  • the driving switch 403 controls the two electric cylinders 106 and the electromagnetic assembly. When the drive switch 403 is pressed for the first time, the slider 105 is driven once, so that the slider 105 is placed in the desired position. Below the cryo-EM body 101, when the drive switch 403 is pressed for the second time, the slider 105 is driven again so that the slider 105 is placed outside the cryo-EM body 101.
  • One stroke drive is a preset stroke path, so as to achieve the purpose of the drive switch 403 driving the adjustment unit.

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Abstract

涉及电镜设备技术领域，具体公开了一种冷冻电镜用冷台及其控制系统，包括冷冻电镜本体（101）和放物机构，放物机构包括底板（102）、法兰环（103）、调节单元、导轨（104）、滑块（105）和样品台，冷冻电镜本体（101）与底板（102）固定连接，并位于底板（102）的上方，法兰环（103）与底板（102）固定连接，并位于底板（102）的下方，导轨（104）与底板（102）固定连接，并位于底板（102）的上方，调节单元的数量为两个，两个调节单元分别设置于底板（102）的上方，且每个调节单元的输出端分别贯穿导轨（104），滑块（105）与导轨（104）滑动连接，并位于导轨（104）的内部，样品台与滑块（105）固定连接。以上结构的设置，可以将样品台进行移动，便于对观测物质进行放置或者更换，使得放置观测的物质位于最佳的状态以及位置。

Description

一种冷冻电镜用冷台及其控制系统 技术领域
本发明涉及电镜设备技术领域，尤其涉及一种冷冻电镜用冷台及其控制系统。
背景技术
目前冷冻电镜是用于对电子光束敏感的生物和高分子材料实现实时观察的设备，其中冷冻电镜的冷源基本上来至于液氦或液氮，但是观察一端时间后就会出现液氮或者液氦耗尽，需要重新进行加注制冷液，导致工作时间短。
现有技术中，通过使用冷头来提供冷源，以达到液氦或者液氮的制冷温度，使用方便，工作时间长。
但现有技术中，由于样品台放置于冷冻电镜本体的下方，并且一部分被冷冻电镜本体遮挡，导致放置观察的物质时会受到阻碍，无法便捷的对观察物进行放置。
发明内容
本发明的目的在于提供一种冷冻电镜用冷台及其控制系统，旨在解决现有技术中的由于样品台放置于冷冻电镜本体的下方，并且一部分被冷冻电镜本体遮挡，导致放置观察的物质时会受到阻碍，无法便捷的对观察物进行放置的技术问题。
为实现上述目的，本发明采用的一种冷冻电镜用冷台，包括冷冻电镜本体和放物机构，所述放物机构包括底板、法兰环、调节单元、导轨、滑块和样品台，所述冷冻电镜本体与所述底板固定连接，并位于所述底板的上方，所述法兰环与所述底板固定连接，并位于所述底板的下方，所述导轨与所述底板固定连接，并位于所述底板的上方，所述调节单元的数量为两个，两个所述调节单元分别设置于所述底板的上方，且每个所述调节单元的输出端分别贯穿所述导轨，所述滑块与所述导轨滑动连接，并位于所述导轨的内部，所述样品台与所 述滑块固定连接，并分别位于所述滑块的上方。
其中，每个所述调节单元包括电缸和电磁组件，所述电缸与所述底板固定连接，并位于所述底板的上方，所述电磁组件与所述电缸的输出端固定连接，并分别位于所述电缸的输出端的一端，且所述电磁组件贯穿所述导轨。
其中，每个所述电磁组件包括主杆体、通电线圈和外筒，所述主杆体与所述电缸的输出端固定连接，并位于所述电缸的输出端的一端，且所述主杆体贯穿所述导轨，所述通电线圈与所述主杆体固定连接，并套设于所述主杆体的外表壁，所述外筒与所述主杆体固定连接，并套设于所述主杆体的外表壁，且所述通电线圈位于所述主杆体与所述外筒之间。
其中，所述样品台包括板体、导冷板、连接套筒和外环，所述板体与所述滑块固定连接，并位于所述滑块的上方，所述导冷板与所述板体固定连接，并位于所述板体的上方，所述连接套筒与所述导冷板固定连接，并位于所述导冷板的外表壁，所述外环与所述导冷板固定连接，并位于所述导冷板的上方。
其中，所述样品台还包括导冷胶，所述导冷胶均匀敷设于所述连接套筒的内表壁。
其中，所述冷冻电镜用冷台还包括导冷机构，所述导冷机构设置于所述底板的上方。
其中，所述导冷机构包括架体、导冷管、接触板、壳体和保温材料，所述架体与所述底板固定连接，并位于所述底板的上方，所述壳体与所述架体固定连接，并位于所述架体的上方，所述导冷管设置于所述架体的内部，且所述导冷管与所述架体之间填充所述保温材料，所述接触板与所述导冷管固定连接，并位于所述导冷管的一端。
其中，所述导冷机构还包括内传管，所述内传管设置于所述导冷管的内部，且所述内传管的一端呈圆角设置。
本发明还提供一种控制系统，包括制冷开关、调温旋钮和驱动开关，所述制冷开关设置于所述底板的上方，所述调温旋钮设置于所述底板的上方，所述驱动开关设置于所述底板的上方，且所述驱动开关与每个所述电磁线圈和所述电缸电性连接。
本发明的一种冷冻电镜用冷台及其控制系统，通过将观测样品放于所述样品台上，并且启动其中一个所述调节单元对所述滑块进行调节，将所述滑块在 所述导轨上进行推动，直至推动至所述导轨的一侧，进而启动另一个所述调节组件进行驱动，将所述滑块推至所述导轨的一侧，使得所述样品台位于所述冷冻电镜本体的正下方进行观测，同时使得所述样品台连接制冷端，以上结构的设置，可以将所述样品台进行移动，便于对观测物质进行放置或者更换，使得放置观测的物质位于最佳的状态以及位置。
附图说明
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。
图1是本发明的第一实施例的结构示意图。
图2是本发明的第一实施例的俯视图。
图3是本发明的第一实施例的正视图。
图4是本发明的图2的A-A线结构剖视图。
图5是本发明的图4的B处局部结构放大图。
图6是本发明的第二实施例的正视图。
图7是本发明的图6的C-C线结构剖视图。
图8是本发明的第三实施例的结构示意图。
101-冷冻电镜本体、102-底板、103-法兰环、104-导轨、105-滑块、106-电缸、107-主杆体、108-通电线圈、109-外筒、110-板体、111-导冷板、112-连接套筒、113-外环、114-导冷胶、201-架体、202-导冷管、203-接触板、204-壳体、205-保温材料、206-内传管、301-环架、302-冷头、303-套环、304-弹簧、305-调节环、306-限位钉、401-制冷开关、402-调温旋钮、403-驱动开关。
具体实施方式
本申请第一实施例为：
请参阅图1至图5，其中图1为第一实施例的结构示意图，图2为第一实施例的俯视图，图3为第一实施例的正视图，图4为图2的A-A线结构剖视图，图5为图4的B处局部结构放大图，本发明提供一种冷冻电镜用冷台：包括冷 冻电镜本体101和放物机构，所述放物机构包括底板102、法兰环103、调节单元、导轨104、滑块105和样品台，所述调节单元包括电缸106和电磁组件，所述电磁组件包括主杆体107、通电线圈108和外筒109，所述样品台包括板体110、导冷板111、连接套筒112、外环113和导冷胶114。
针对本具体实施方式，通过所述电缸106驱动所述电磁组件进行移动，使用所述电缸106与所述电磁组件的配合对所述滑块105进行移动，并使得所述样品台进行移动，所述导冷板111和所述连接套筒112可以对低温进行传导，以此对观测样品进行冷冻。
其中，所述冷冻电镜本体101与所述底板102固定连接，并位于所述底板102的上方，所述法兰环103与所述底板102固定连接，并位于所述底板102的下方，所述导轨104与所述底板102固定连接，并位于所述底板102的上方，所述调节单元的数量为两个，两个所述调节单元分别设置于所述底板102的上方，且每个所述调节单元的输出端分别贯穿所述导轨104，所述滑块105与所述导轨104滑动连接，并位于所述导轨104的内部，所述样品台与所述滑块105固定连接，并分别位于所述滑块105的上方，将观测样品放于所述样品台上，并且启动其中一个所述调节单元对所述滑块105进行调节，将所述滑块105在所述导轨104上进行推动，直至推动至所述导轨104的一侧，进而启动另一个所述调节组件进行驱动，将所述滑块105推至所述导轨104的一侧，使得所述样品台位于所述冷冻电镜本体101的正下方进行观测，同时使得所述样品台连接制冷端，其中所述法兰环103用于对所述冷冻电镜用冷台进行安装固定。
其次，所述电缸106与所述底板102固定连接，并位于所述底板102的上方，所述电磁组件与所述电缸106的输出端固定连接，并分别位于所述电缸106的输出端的一端，且所述电磁组件贯穿所述导轨104，所述电缸106的伸缩驱动可以将所述电磁组件进行伸缩，同时通过电磁组件对所述滑块105进行电磁磁吸，达到移动调节所述滑块105的目的。
同时，所述主杆体107与所述电缸106的输出端固定连接，并位于所述电缸106的输出端的一端，且所述主杆体107贯穿所述导轨104，所述通电线圈108与所述主杆体107固定连接，并套设于所述主杆体107的外表壁，所述外筒109与所述主杆体107固定连接，并套设于所述主杆体107的外表壁，且所述通电线圈108位于所述主杆体107与所述外筒109之间，所述外筒109对所述通 电线圈108进行保护，同时在移动所述滑块105时，对所述通电线圈108进行通电，进而使得所述主杆体107具备电磁力，使得所述主杆体107对所述滑块105进行磁吸固定。
另外，所述板体110与所述滑块105固定连接，并位于所述滑块105的上方，所述导冷板111与所述板体110固定连接，并位于所述板体110的上方，所述连接套筒112与所述导冷板111固定连接，并位于所述导冷板111的外表壁，所述外环113与所述导冷板111固定连接，并位于所述导冷板111的上方，所述连接套筒112用于连接传冷设备，所述板体110对所述导冷板111进行支撑，同时所述外环113对所述导冷板111进行压持，同时形成可以装载观测物的容器，其中所述导冷板111与所述连接套筒112均采用铜或者银制成。
进而，所述导冷胶114均匀敷设于所述连接套筒112的内表壁，所述导冷胶114的敷设可以在连接导冷设备时消除连接间隙，增强低温的传导。
使用本实施例的一种冷冻电镜用冷台时，将观测样品放于所述样品台上，并且启动其中一个所述调节单元对所述滑块105进行调节，将所述滑块105在所述导轨104上进行推动，直至推动至所述导轨104的一侧，进而启动另一个所述调节组件进行驱动，将所述滑块105推至所述导轨104的一侧，使得所述样品台位于所述冷冻电镜本体101的正下方进行观测，同时使得所述样品台连接制冷端，在所述调节单元之中，所述电缸106的伸缩驱动可以将所述电磁组件进行伸缩，同时通过电磁组件对所述滑块105进行电磁磁吸，达到移动调节所述滑块105的目的，在所述电磁组件之中，所述外筒109对所述通电线圈108进行保护，同时在移动所述滑块105时，对所述通电线圈108进行通电，进而使得所述主杆体107具备电磁力，使得所述主杆体107对所述滑块105进行磁吸固定，在所述样品台之中，所述连接套筒112用于连接传冷设备，所述板体110对所述导冷板111进行支撑，同时所述外环113对所述导冷板111进行压持，同时形成可以装载观测物的容器，其中所述导冷板111与所述连接套筒112均采用铜或者银制成，所述导冷胶114的敷设可以在连接导冷设备时消除连接间隙，增强低温的传导。
本申请第二实施例为：
在第一实施例的基础上，请参阅图6和图7，其中图6为第二实施例的正视图，图7为图6的C-C线结构剖视图，本发明提供一种冷冻电镜用冷台：还包 括导冷机构，所述导冷机构包括架体201、导冷管202、接触板203、壳体204、保温材料205和内传管206。
针对本具体实施方式，通过所述接触板203进行传导低温，所述壳体204对所述保温材料205和所述导冷管202进行保护，所述保温材料205可以防止温度的流失。
其中，所述架体201与所述底板102固定连接，并位于所述底板102的上方，所述壳体204与所述架体201固定连接，并位于所述架体201的上方，所述导冷管202设置于所述架体201的内部，且所述导冷管202与所述架体201之间填充所述保温材料205，所述接触板203与所述导冷管202固定连接，并位于所述导冷管202的一端，所述架体201对所述壳体204进行支撑，同时所述壳体204对所述保温材料205和所述导冷管202进行包裹，所述保温材料205可以防止所述导冷管202在对低温进行传导时减少温度的流失，所述接触板203用于对制冷设备的输出端进行接触，以此达到导冷的目的。
其次，所述内传管206设置于所述导冷管202的内部，且所述内传管206的一端呈圆角设置，所述内传管206采用铜或银制成，使得导冷效率更高，同时采用圆角设置，可以更好的便于所述连接套筒112导入。
使用本实施例的一种冷冻电镜用冷台时，所述架体201对所述壳体204进行支撑，同时所述壳体204对所述保温材料205和所述导冷管202进行包裹，所述保温材料205可以防止所述导冷管202在对低温进行传导时减少温度的流失，所述接触板203用于对制冷设备的输出端进行接触，以此达到导冷的目的，所述内传管206采用铜或银制成，使得导冷效率更高，同时采用圆角设置，可以更好的便于所述连接套筒112导入。
本申请第三实施例为：
在第二实施例的基础上，请参阅图8，其中图8为第三实施例的结构示意图，本发明提供一种冷冻电镜用冷台：还包括制冷机构，所述制冷机构包括环架301、冷头302、套环303、弹簧304、调节环305和限位钉306。
针对本具体实施方式，通过所述冷头302进行制造低温，并通过所述接触板203进行传导，所述冷头302通过所述环架301设置于所述接触板203的上方，同时通过所述调节环305对所述套环303的高度进行调节，以此来对所述冷头302的高度进行调节。
其中，所述环架301与所述壳体204固定连接，并套设于所述壳体204的外表壁，所述套环303的数量为多个，每个所述套环303分别与所述冷头302固定连接，并分别位于所述冷头302的外表壁，且每个所述套环303均套设于所述环架301的外表壁，所述弹簧304的数量为多根，每根所述弹簧304分别套设于所述环架301的外表壁，并分别位于对应的所述套环303的下方，所述调节环305的数量为多个，每个所述调节环305分别与所述环架301螺纹连接，并分别套设于所述环架301的外表壁，所述冷头302进行制造低温，并通过所述接触板203进行传导，所述冷头302通过所述环架301设置于所述接触板203的上方，同时通过所述调节环305对所述套环303的高度进行调节，以此来对所述冷头302的高度进行调节，同时所述弹簧304通过弹力可以使得所述调节环305卡持得更紧，使得所述调节环305不易发生松动。
其次，所述限位钉306的数量为多个，每个所述限位钉306分别与所述环架301螺纹连接，并分别位于所述环架301的上方，所述限位钉306可以防止所述调节环305脱离，起到有效的保护作用。
使用本实施例的一种冷冻电镜用冷台时，所述冷头302进行制造低温，并通过所述接触板203进行传导，所述冷头302通过所述环架301设置于所述接触板203的上方，同时通过所述调节环305对所述套环303的高度进行调节，以此来对所述冷头302的高度进行调节，同时所述弹簧304通过弹力可以使得所述调节环305卡持得更紧，使得所述调节环305不易发生松动，所述限位钉306可以防止所述调节环305脱离，起到有效的保护作用。
本发明还提供一种控制系统，包括制冷开关401、调温旋钮402和驱动开关403，所述制冷开关401设置于所述底板102的上方，所述调温旋钮402设置于所述底板102的上方，所述驱动开关403设置于所述底板102的上方，且所述驱动开关403与每个所述电磁线圈和所述电缸106电性连接。
所述制冷开关401对所述冷头302进行控制，对所述冷头302进行开启与关闭，同时通过所述调温旋钮402使得所述冷头302制造对应所需的稳定，所述驱动开关403即对两个所述电缸106以及所述电磁组件进行控制，当按下所述驱动开关403第一次，即将所述滑块105做一次行程驱动，使得所述滑块105置于所述冷冻电镜本体101的下方，当按下所述驱动开关403第二次，即对所述滑块105再做一次行程驱动，使得所述滑块105置于所述冷冻电镜本体101 的外部，其中一次行程驱动是预设的行程路径，以此达到所述驱动开关403驱动所述调节单元的目的。
以上所揭露的仅为本发明一种较佳实施例而已，当然不能以此来限定本发明之权利范围，本领域普通技术人员可以理解实现上述实施例的全部或部分流程，并依本发明权利要求所作的等同变化，仍属于发明所涵盖的范围。

Claims (9)

  1. 一种冷冻电镜用冷台，包括冷冻电镜本体，其特征在于，
    还包括放物机构；
    所述放物机构包括底板、法兰环、调节单元、导轨、滑块和样品台，所述冷冻电镜本体与所述底板固定连接，并位于所述底板的上方，所述法兰环与所述底板固定连接，并位于所述底板的下方，所述导轨与所述底板固定连接，并位于所述底板的上方，所述调节单元的数量为两个，两个所述调节单元分别设置于所述底板的上方，且每个所述调节单元的输出端分别贯穿所述导轨，所述滑块与所述导轨滑动连接，并位于所述导轨的内部，所述样品台与所述滑块固定连接，并分别位于所述滑块的上方。
  2. 如权利要求1所述的一种冷冻电镜用冷台，其特征在于，
    每个所述调节单元包括电缸和电磁组件，所述电缸与所述底板固定连接，并位于所述底板的上方，所述电磁组件与所述电缸的输出端固定连接，并分别位于所述电缸的输出端的一端，且所述电磁组件贯穿所述导轨。
  3. 如权利要求2所述的一种冷冻电镜用冷台，其特征在于，
    每个所述电磁组件包括主杆体、通电线圈和外筒，所述主杆体与所述电缸的输出端固定连接，并位于所述电缸的输出端的一端，且所述主杆体贯穿所述导轨，所述通电线圈与所述主杆体固定连接，并套设于所述主杆体的外表壁，所述外筒与所述主杆体固定连接，并套设于所述主杆体的外表壁，且所述通电线圈位于所述主杆体与所述外筒之间。
  4. 如权利要求3所述的一种冷冻电镜用冷台，其特征在于，
    所述样品台包括板体、导冷板、连接套筒和外环，所述板体与所述滑块固定连接，并位于所述滑块的上方，所述导冷板与所述板体固定连接，并位于所述板体的上方，所述连接套筒与所述导冷板固定连接，并位于所述导冷板的外表壁，所述外环与所述导冷板固定连接，并位于所述导冷板的上方。
  5. 如权利要求4所述的一种冷冻电镜用冷台，其特征在于，
    所述样品台还包括导冷胶，所述导冷胶均匀敷设于所述连接套筒的内表壁。
  6. 如权利要求5所述的一种冷冻电镜用冷台，其特征在于，
    所述冷冻电镜用冷台还包括导冷机构，所述导冷机构设置于所述底板的上 方。
  7. 如权利要求6所述的一种冷冻电镜用冷台，其特征在于，
    所述导冷机构包括架体、导冷管、接触板、壳体和保温材料，所述架体与所述底板固定连接，并位于所述底板的上方，所述壳体与所述架体固定连接，并位于所述架体的上方，所述导冷管设置于所述架体的内部，且所述导冷管与所述架体之间填充所述保温材料，所述接触板与所述导冷管固定连接，并位于所述导冷管的一端。
  8. 如权利要求7所述的一种冷冻电镜用冷台，其特征在于，
    所述导冷机构还包括内传管，所述内传管设置于所述导冷管的内部，且所述内传管的一端呈圆角设置。
  9. 一种控制系统，控制如权利要求8所述的一种冷冻电镜用冷台，其特征在于，
    包括制冷开关、调温旋钮和驱动开关，所述制冷开关设置于所述底板的上方，所述调温旋钮设置于所述底板的上方，所述驱动开关设置于所述底板的上方，且所述驱动开关与每个所述电磁线圈和所述电缸电性连接。
PCT/CN2022/120458 2022-07-28 2022-09-22 一种冷冻电镜用冷台及其控制系统 WO2024021269A1 (zh)

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