WO2024016895A1 - 细胞处理设备 - Google Patents

细胞处理设备 Download PDF

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Publication number
WO2024016895A1
WO2024016895A1 PCT/CN2023/099698 CN2023099698W WO2024016895A1 WO 2024016895 A1 WO2024016895 A1 WO 2024016895A1 CN 2023099698 W CN2023099698 W CN 2023099698W WO 2024016895 A1 WO2024016895 A1 WO 2024016895A1
Authority
WO
WIPO (PCT)
Prior art keywords
centrifugal
centrifuge
cell processing
weighing
component
Prior art date
Application number
PCT/CN2023/099698
Other languages
English (en)
French (fr)
Inventor
方汝林
陈志林
朱俊枢
赵慧敏
商院芳
郭霄亮
Original Assignee
深圳赛桥生物创新技术有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 深圳赛桥生物创新技术有限公司 filed Critical 深圳赛桥生物创新技术有限公司
Publication of WO2024016895A1 publication Critical patent/WO2024016895A1/zh

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B15/00—Other accessories for centrifuges
    • B04B15/02—Other accessories for centrifuges for cooling, heating, or heat insulating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
    • B04B9/02—Electric motor drives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01G—WEIGHING
    • G01G17/00—Apparatus for or methods of weighing material of special form or property
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01G—WEIGHING
    • G01G21/00—Details of weighing apparatus
    • G01G21/02—Arrangements of bearings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01G—WEIGHING
    • G01G21/00—Details of weighing apparatus
    • G01G21/02—Arrangements of bearings
    • G01G21/10—Floating suspensions; Arrangements of shock absorbers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2846—Cytocentrifuge method

Definitions

  • the present application belongs to the field of cell processing technology, and particularly relates to a cell processing equipment.
  • cell processing is usually performed through cell processing equipment, such as cell separation or mixing.
  • Cell processing equipment in the prior art usually has a single function and is large in size.
  • This application provides a cell processing equipment to address the technical problems of single function and low weighing efficiency of cell processing equipment in the prior art.
  • a cell processing device including:
  • a weighing device includes a support component and at least one group of weighing components.
  • the support component includes a bracket for supporting the weighing component.
  • Each group of the weighing components includes a mounting bracket installed on the bracket.
  • a centrifugal device including a centrifugal pot with a centrifugal chamber, a centrifugal cup arranged in the centrifugal chamber, and a centrifugal module connected to the centrifugal pot and used to drive the centrifugal cup to rotate;
  • a liquid circuit module including a pipeline component for transporting liquid in the sample to the centrifuge cup for cell processing, and a liquid circuit panel for installing and controlling the flow of liquid in the pipeline component;
  • the centrifugal pot, the liquid path panel and the bracket are all installed on the housing.
  • the weighing device is provided with at least one set of weighing components.
  • the weighing component is provided with hooks for hanging samples, which facilitates hanging the samples and prevents the flow of liquid in the suspended samples from being blocked; and when weighing multiple samples at the same time, since each hook is hung
  • the samples are all weighed by their correspondingly configured weighing sensors. Therefore, the weighing results of each sample will be independent of each other without interfering with each other.
  • each set of weighing components in this application is installed through a mounting bracket.
  • this application uses a centrifuge module specially configured for the centrifuge cup to install the centrifuge cup and drive the centrifuge cup to rotate, making the installation of the centrifuge cup more convenient and firm, avoiding the centrifuge cup falling off, and ensuring the safety of the cell processing process. sex.
  • the cell processing equipment of the present application has a simple structure, which reduces the volume of the equipment; and each part is independently and removably installed on the casing. When a fault occurs, the troubleshooting process is simplified and maintenance is facilitated.
  • Figure 1 is a schematic structural diagram of a cell processing device provided by an embodiment of the present application.
  • Figure 2 is an exploded structural diagram of a weighing device of a cell processing equipment provided by an embodiment of the present application.
  • Figure 3 is an exploded structural schematic diagram of the weighing component of the cell processing equipment provided by an embodiment of the present application.
  • Figure 4 is a schematic structural diagram of a hook of a cell processing device provided by an embodiment of the present application.
  • Figure 5 is a schematic structural diagram of a hook of a cell processing device provided by another embodiment of the present application.
  • Figure 6 is a schematic diagram of the assembly structure of the centrifugal module of the cell processing equipment provided by an embodiment of the present application.
  • Figure 7 is a schematic exploded structural diagram of a centrifugal module of a cell processing equipment provided by an embodiment of the present application.
  • Figure 8 is a schematic exploded structural view of the centrifugal module and the temperature control module of the cell processing equipment provided by an embodiment of the present application.
  • Figure 9 is a schematic diagram of the assembly structure of the centrifugal module and the temperature control module of the cell processing equipment provided by an embodiment of the present application.
  • Figure 10 is a schematic structural diagram of a liquid circuit module of a cell processing equipment provided by an embodiment of the present application.
  • Bearing assembly 1021, bearing seat; 1022, bearing; 103, coupling; 104, drive motor; 105, brake; 1051, through hole; 106, rotating mounting seat; 107, irregular wheel; 108, motor mounting seat; 1081. Motor accommodation space; 109. Fixed plate; 3. Liquid circuit module; 31. Pipeline components; 311. Pinch valve; 312. Bubble sensor; 313. Pressure sensor; 314. Peristaltic pump; 315. Stepper motor; 32. Liquid circuit panel; 4. Sample; 5. Housing; 51. Start button; 6. Scanning device; 7. Display device.
  • one embodiment of the present application provides a cell processing device, including:
  • the weighing device 1 includes a support component 11 and at least one group of weighing components 12.
  • the support component 11 includes a bracket 111 for supporting the weighing component 12; each group of the weighing components 12 includes a
  • the mounting bracket 121 on the bracket 111, the load cell 122 installed on the mounting bracket 121, and the load cell 122 can be detachably installed on the load cell 122 to suspend the sample 4 (such as a load cell for holding liquids).
  • the hook 123 of the liquid bag); wherein, the load sensor 122 can be a pressure sensing component that determines the weight through a pressure signal.
  • Both the bracket 111 and the mounting bracket 121 can be made of sheet metal parts, so that the bracket 111 is stronger and can better bear the load.
  • the mounting bracket 121 of the sheet metal piece that is not easily deformed can be better used.
  • Protect the load cell 122, etc. The shape and size of the bracket 111 can be set according to requirements, as long as the weighing component 12 can be stably installed on the bracket 111 and the volume of the entire weighing device 1 is reduced as much as possible.
  • the weighing device 1 is provided with a group or There are multiple groups of weighing components 12 with the same structure (the number of weighing components 12 can be set according to requirements). Each group of weighing components 12 is installed on the bracket 111 through the mounting bracket 121, so a single group of weighing components can be realized.
  • the hook 123 also adopts a structural design that is detachably connected to the load cell 122. Therefore, it facilitates the disassembly and installation of the hook 123 and also improves the efficiency of troubleshooting.
  • the load cell 122 is provided with a threaded hole (not shown)
  • the hook 123 is provided with a connecting thread 1231
  • the hook 123 is threaded with the threaded hole through the connecting thread 1231. connect. That is, in this embodiment, the detachable connection between the hook 123 and the load cell 122 is achieved through the threaded connection between the connecting thread 1231 and the threaded hole.
  • the mounting bracket 121 is provided with an accommodating groove 1211 that is adapted to the load cell 122, and the inner wall of the accommodating groove 1211 is provided with a fixing hole 1212. , the load cell 122 is fixedly installed in the accommodating groove 1211 through screws passing through the fixing holes 1212 . That is, the mounting bracket 121 can be a bent plate that is bent to form the accommodating groove 1211. The load cell 122 is installed in the accommodating groove 1211 and can be protected from damage by the mounting bracket 121.
  • the fixing hole 1212 in this embodiment is provided on the top inner wall of the accommodating groove 1211, so that the top of the load cell 122 is not easily fixed to the mounting bracket 121. Take it out from the accommodating groove 1211.
  • the mounting bracket 121 includes a flange 1213 provided on the edge of the accommodating groove 1211, and the mounting bracket 121 is installed on the bracket 111 through the flange 1213.
  • the installation support 121 can be fixed on the installation plate 1111 through the above-mentioned flange 1213, and the connection method between the two can be a detachable connection method such as screw connection, snap connection, etc., so that the installation can be removed from the installation plate 1111.
  • the support 121 and the entire weighing assembly 12 are detached.
  • the centrifuge device 2 includes a centrifuge pot 21 with a centrifuge chamber, a centrifuge cup (not shown) disposed in the centrifuge chamber, and a centrifuge module 100 connected to the centrifuge pot 21 and used to drive the centrifuge cup to rotate; wherein , the centrifuge module 100 of the centrifuge device 2 drives the centrifuge cup located in the centrifuge chamber of the centrifuge pot 21 to rotate, so as to perform cell processing on the liquid injected in the centrifuge cup.
  • the centrifuge cup is installed through a centrifuge module 100 specially configured for the centrifuge cup and drives the centrifuge cup to rotate, making the installation of the centrifuge cup more convenient and firm, avoiding the centrifuge cup falling off, and ensuring cell processing. Process safety.
  • the liquid circuit module 3 includes a pipeline component 31 for transporting the liquid in the sample 4 to the centrifuge cup for cell processing, and a liquid pipeline component for installing and controlling the flow of liquid in the pipeline component 31.
  • Road panel 32 that is, in the above-mentioned liquid channel module 3, as shown in Figure 10, the pipeline assembly 31 is installed on the liquid channel panel 32, and by operating and controlling the above-mentioned pipeline assembly 31 on the liquid channel panel 32, you can This allows the liquid in sample 4 to flow smoothly into the centrifuge cup with the pipeline assembly 31 for cell processing.
  • the housing 5 the centrifuge pot 21 , the liquid path panel 32 and the bracket 111 are all installed on the housing 5 . Understandably, the shape of the housing 5 can be set according to needs and is not limited to that shown in Figure 1.
  • the housing 5 includes a support frame, a bottom plate installed at the bottom of the support frame, and side plates installed around the support frame. , the top plate installed on the top of the supporting frame.
  • the above-mentioned bottom plate, top plate and side plate can be assembled together by means of screw connection, snap connection or welding, etc.
  • the bottom plate, top plate and side plate can be detachably connected to the support frame to facilitate assembly from the disassembled position.
  • the components inside the housing 5 are installed and maintained (such as intubation, replacement of centrifuge cups, etc.).
  • the weighing device 1 of the cell processing equipment of the present application is provided with at least one set of weighing components 12. Through the above-mentioned weighing components 12, one or more samples 4 suspended on the hook 123 can be weighed and tested at the same time, thereby improving the weighing efficiency. Efficiency, at the same time, the weighing assembly 12 is provided with a hook 123 for hanging the sample 4, which facilitates hanging the sample 4 and prevents the flow of liquid in the suspended sample 4 from being hindered; and, multiple samples 4 are processed at the same time.
  • each group of weighing components 12 is installed on the bracket 111 through the mounting bracket 121, so the disassembly and installation of a single group of weighing components 12 can be realized, which facilitates inspection and maintenance.
  • this application uses a centrifuge module 100 specially configured for the centrifuge cup to install the centrifuge cup and drive the centrifuge cup to rotate. It makes the installation of the centrifuge cup more convenient and firm, avoids the centrifuge cup falling off, and ensures the safety of the cell processing process.
  • the cell processing equipment of the present application has a simple structure, which reduces the volume of the equipment; and each part is independently and removably installed on the housing 5. When a fault occurs, the troubleshooting process is simplified and maintenance is facilitated.
  • the weighing components 12 are in at least two groups; the bracket 111 includes a mounting plate 1111 provided with mounting holes 1112 at intervals (preferably evenly spaced).
  • the number of mounting holes 1112 is consistent with the number of the weighing components 12; the mounting support 121 installs the load sensor 122 on the mounting plate 1111 at a position opposite to the mounting holes 1112.
  • the hook 123 passes through the mounting hole 1112 to connect to the load sensor 122 . That is, in this embodiment, each mounting hole 1112 is used to install a set of weighing components 12. It is understandable that the above-mentioned mounting holes 1112 can also be designed as slots, as long as the hook 123 can pass through to connect with the weighing assembly.
  • the sensor 122 is connected, and the installation of the mounting bracket 121 on the mounting plate 1111 is ensured to be stable and reliable.
  • the bracket 111 also includes a support rod 1113 connected to the mounting plate 1111.
  • the support assembly 11 also includes a front shell 112 and a rear shell 113.
  • the front shell 112 and the rear shell 113 enclose a Installation space for installing the support rod 1113. That is, the support rod 1113 is used to support the installation plate 1111 and all the weighing components 12 installed on the installation plate 1111 to maintain the stability of the weighing components 12 during weighing.
  • the front shell 112 and the rear shell 113 are used to protect the support rod 1113.
  • the weighing assembly 12 further includes a front cover 124 and a back cover 125, and an area is enclosed between the front cover 124 and the back cover 125 for accommodating the mounting plate 1111 and the mounting bracket. seat 121 and the accommodation space of the load cell 122. That is, after the weighing assembly 12 is installed on the mounting plate 1111, the front cover 124 and the rear cover 125 further protect the mounting plate 1111 and the load sensor 122 in the weighing assembly 12 in the accommodation space to avoid damage.
  • the hook 123 includes a bending section 1232, a lateral deflection section 1233 and a hooking section 1234 connected to the load cell 122 (understandably, the above-mentioned connecting thread 1231 is It can be an external thread provided on the hooking section 1234), and the opposite ends of the bending section 1232 are respectively connected to the hooking section 1234 and the lateral deflection section 1233; the bending section 1232 is connected to the lateral deviation section 1233.
  • the lateral deflection section 1233 forms a hooking ring with an opening 1235; the central axis L1 of the bending section 1232 and the hooking section are both located on the first preset reference plane, and the central axis L1 of the lateral deflection section 1233 It is set at a preset side slip angle a with the first preset reference plane.
  • the bending section 1232, the lateral deviation section 1233, and the hooking section 1234 are integrally formed structures, which can simplify the manufacturing process of the hook 123, improve preparation efficiency, and reduce manufacturing costs.
  • the bending section 1232, the lateral deflection section 1233 and the hooking section 1234 can be detachably connected, which facilitates storage and transportation.
  • the material of the hook 123 may preferably be metal, such as stainless steel. It is understandable that the bending shape and bending angle of the bending section 1232 can be set according to requirements. In this embodiment, the bending section 1232 and the central axis L1 of the hooking section are both located in the same plane (also That is, both are located in the first preset reference plane. Further, when the hook 123 is detachably connected to the load cell 122, the first preset reference plane is perpendicular to the horizontal plane).
  • bending Part of the central axis of the segment 1232 may be located in the first preset reference plane, and the other part may be bent and extended outside the first preset reference plane.
  • shape of the hooking ring changes with the shape of the lateral deviation section 1233 and the bending section 1232, and is not limited in this application.
  • the connection point between the lateral deviation section 1233 and the bending section 1232 is located on the first preset reference plane. Starting from this connection point, the lateral deviation section 1233 is laterally offset in a direction away from the first preset reference plane.
  • the distance between the lateral deflection section 1233 and the first preset reference plane gradually increases in the direction from the connection point toward the end away from the bending section 1232 .
  • the side deflection section 1233 is not necessarily a straight line, it can be a curve (the central axis of the side deflection section 1233 can be fitted to a straight line according to its bending direction, etc.), as long as it faces away from The direction of the first preset reference plane deviates and the deviation angle reaches the preset deviation angle.
  • the sample 4 (not shown) can be hung forward from the opening 1235 of the hook ring (the wider side of the sample 4 faces the operating table of the cell processing device) into the hook ring, and because The central axis of the side deflection section 1233 is set at a preset side deflection angle a with the first preset reference plane. Therefore, when the sample 4 relies on its own gravity, it moves along the side deflection section 1233 When falling to the bottom of the hook ring, the sample 4 will be naturally suspended sideways (the narrower side of the sample 4 faces the operating table) on the hook 123, thereby reducing the space occupied by the sample 4 arranged on the support assembly 11 of the cell processing equipment.
  • the lateral width of the sample 4 allows more samples 4 to be hung on the same support component 11, thereby improving the operating efficiency; and in the process of hanging the sample 4 through the hook 123 of the present application, the operator can directly hang the sample from the front. 4.
  • the obtained laterally arranged sample 4 saves time and effort, and greatly improves the user operating experience.
  • the preset side slip angle a is 30-60 degrees. Preferably, the preset slip angle a is 45 degrees.
  • the preset side deflection angle a is set to belong to the above angle range, which allows the operator to hang the sample 4 from the forward direction of the operating console, which improves the convenience of operation and also allows the operator to hang the sample 4 from the forward direction.
  • the final sample 4 relies on its own gravity to fall along the lateral deflection section 1233 in the hook ring to achieve lateral arrangement, thereby reducing the lateral width occupied by the samples 4 arranged on the support assembly 11 of the cell processing equipment, so that the same support More samples 4 can be hung on the component 11, which improves operating efficiency.
  • the central axis L1 of the hitch section passes through the connection point between the bending section 1232 and the lateral deflection section 1233; the first relative distance is greater than Or equal to the second relative distance; wherein the first relative distance refers to the relative distance between the connection point and the second preset reference plane; the second preset reference plane is perpendicular to the hitch section 1234 and passes through any point on the hitch section 1234; the second relative distance refers to other points on the bending section 1232 and the lateral offset section 1233, except for the connection point, and the third relative distance.
  • the relative distance between the two preset datum planes are provided.
  • the hooking section 1234 and the first preset reference plane where it is located are both perpendicular to the horizontal plane, because the second preset reference plane is perpendicular to the hooking section. Section 1234. Therefore, at this time, the second preset reference plane is a plane parallel to the horizontal plane, and the second reference plane passes through any point on the hooking section 1234. For example, the second reference plane passes through the hooking section. 1234 and the adjacent point of the bending section 1232. At this time, since the first relative distance is greater than or equal to the second relative distance, the connection point between the bending section 1232 and the side deflection section 1233 is the entire hook 123 The current position of the installation state is the lowest point.
  • the sample 4 that is hung from the opening 1235 of the hook ring on the hook 123 will fall into the bending section 1232 after falling naturally under its own gravity. and the side deflection section 1233, and the connection point is located directly below the central axis L1 of the hook section. Therefore, after the sample 4 slides to the connection point position, the hook section 1234 of the hook 123 remains It will remain perpendicular to the horizontal plane and will not shift or shake under the gravity of sample 4, thereby ensuring the stability of the cell processing equipment.
  • the bending section 1232 includes a straight section parallel to the central axis L1 of the hooking section. The above arrangement can make the structure of the hook 123 more stable and reliable.
  • the centrifugal module 100 includes a rotating shaft 101, a bearing assembly 102, a coupling 103, a driving motor 104 for driving the rotating shaft 101 to rotate.
  • the brake 105 that brakes the rotating shaft 101 and the rotating mounting seat 106 located in the centrifugal chamber and used to install the centrifuge cup; the coupling 103 is connected to the output end of the driving motor 104 Between the rotary shaft 101 and the rotary shaft 101 , one end of the rotary shaft 101 away from the coupling 103 passes through the bearing assembly 102 and is connected to the rotary mounting base 106 .
  • the brake 105 is connected to the bearing assembly 102 .
  • the rotating mounting base 106 is installed on the rotating shaft 101 through pins 1012. Specifically, a pin hole 1013 is opened at one end of the rotating shaft 101 away from the coupling 103, and the rotating mounting base 106 is inserted into the pin hole 1013. After the pin 1012, it is fixed on the rotating shaft 101 through screws; further, the rotating mounting base 106 is provided with a first engaging portion, and the centrifugal cup is provided with a second engaging portion, and the centrifugal cup is provided with the first engaging portion. The centrifugal cup and the rotating mounting base 106 can be further fixedly connected through a screw assembly to stabilize the connection between the two.
  • the rotary mounting base 106 is provided to make the centrifuge module 100 compatible with centrifuge cups of various specifications.
  • the driving motor 104 is a brushless DC motor to increase the service life of the motor of the centrifugal module 100 and reduce noise.
  • the coupling 103 is a diaphragm coupling. Since the diaphragm coupling has no backlash, the use of the diaphragm coupling can further improve the transmission accuracy of the centrifugal module 100. It can be understood that the brake 105 brakes the rotating shaft 101 through the brake disc when the power is turned off; and the brake 105 releases the brake disc to release the braking state of the rotating shaft 101 when the power is turned on. At this time, the driving motor 104 can operate.
  • the coupling 103 drives the rotating shaft 101 to rotate, and the rotating shaft 101 drives the rotating mounting base 106
  • the centrifuge cup on the centrifuge cup rotates to achieve cell processing such as separation, mixing, preparation and cleaning of the sample 4 (such as blood) components in the centrifuge cup.
  • the rotating shaft 101 is rotatably mounted on the bearing assembly 102 to limit the radial runout range of the rotating shaft 101 and reduce the radial runout when the centrifugal module 100 drives the centrifuge cup to rotate; at the same time, the rotating shaft 101 passes through the coupling
  • the brake 103 connects the rotating shaft 101 and the driving motor 104, and brakes and drives the rotating shaft 101 respectively through the brake 105 and the driving motor 104.
  • the rotating mounting base 106 is specially adapted to install the centrifuge cup, making the installation of the centrifuge cup more convenient and firm, avoiding the centrifugal cup falling off when the rotating shaft 101 drives the centrifuge cup to rotate through the rotating mounting base 106, and ensuring cell processing.
  • the safety of the process and the reliable quality of cell processing result in better results.
  • the bearing assembly 102 includes a bearing seat 1021 with a mounting through hole and two bearings 1022 mounted on the inner wall of the mounting through hole; the rotating shaft 101 passes through two
  • the bearing 1022 is installed on the bearing seat 1021; the brake 105 is installed on the bearing seat 1021.
  • the brake 105 is provided with a through hole 1051. After the rotation shaft 101 passes through the through hole 1051, it is rotationally connected to the bearing seat 1021 through the two bearings 1022. Further, the brake 105 is fixedly installed with screws. on the bearing seat 1021, and the outer rings of the two bearings 1022 are interference-fitted on the inner side walls of the installation through holes in the bearing base 1021.
  • the inner side walls of the installation through holes are recessed to form parallel Two annular grooves (not shown), and the two bearings 1022 are respectively embedded in the two annular grooves.
  • the rotating shaft 101 passes through the through hole 1051 on the brake 105, it passes through the two bearings. 1022 to be rotationally connected to the bearing seat 1021 through two bearings 1022; optionally, the rotating shaft 101 is provided with a positioning step 1011, and the two bearings 1022 are installed on the positioning step 1011 to Fix the inner ring of the bearing 1022 stably on the positioning step 1011.
  • the bearing assembly 102 with double bearings 1022 is used to limit the position of the rotating shaft 101, which limits the radial runout range of the rotating shaft 101 to the greatest extent.
  • the centrifugal module 100 also includes an irregular wheel 107 installed on the rotating shaft 101, and the brake 105 is provided with a brake that is adapted to the irregular wheel 107. card slot (not shown).
  • the shape of the irregular wheel 107 can be set according to requirements.
  • the irregular wheel 107 can be a square wheel.
  • the clamping groove will be a square groove adapted to the square wheel.
  • the square groove will not brake the rotating shaft 101 connected to the square wheel; and after the brake 105 brakes, the brake disc first brakes the rotating shaft 101 connected to the square wheel.
  • the rotating shaft 101 is braked. After that, the rotation speed of the rotating shaft 101 is reduced, and the square wheel will also be inserted into the slot along with the axial movement of the rotating shaft 101, causing the rotating shaft 101 to quickly stop moving.
  • the centrifugal module 100 further includes a motor mounting base 108 having a motor accommodation space 1081, and the driving motor 104 is installed in the motor accommodation space 1081. ;
  • the bearing assembly 102 is installed on the motor mounting base 108.
  • the drive motor 104 is installed and placed in the motor accommodation space 1081 formed by the sunken platform on the motor mounting base 108.
  • the inner wall of the motor accommodation space 1081 is provided with a central axis hole, and the output end (also That is, the output shaft) passes through the central shaft hole and is connected to the coupling 103.
  • the driving motor 104 will be fixedly installed (for example, connected by screws) in the sinking platform.
  • the centrifugal module 100 further includes a fixing plate 109 for connecting external components.
  • the end of the motor mounting seat 108 away from the bearing seat 1021 is installed on the fixing plate 109 . That is to say, the open end of the sink platform on the motor mounting base 108 (the open end of the motor accommodation space 1081) is connected and fixed with the fixing plate 109, and then the entire centrifugal module 100 can be fixed on an external component with the fixing plate 109, such as , fixed at the bottom of the centrifuge chamber of the centrifuge pot 21 of the cell processing device, so that the centrifuge cup can be driven by the centrifuge module 100 in the centrifuge chamber to perform cell processing operations.
  • the centrifuge device 2 further includes a temperature control module 22 for controlling the temperature of the centrifuge chamber.
  • the temperature control module 22 includes a temperature control module for controlling the temperature of the centrifuge chamber.
  • the refrigeration component 211 for cooling the centrifugal cavity, and the heat dissipation component 222 for dissipating heat from the refrigeration component 211; the refrigeration surface of the refrigeration component 211 is attached to the outer wall of the centrifugal pot 21, and the refrigeration component The heating surface of 211 is connected to the heat dissipation component 222.
  • the refrigeration component 211 can be at least one semiconductor refrigeration chip with a cooling surface attached to the inner wall of the centrifuge pot 21.
  • the semiconductor refrigeration chip After being powered on, the semiconductor refrigeration chip can cool the centrifuge cavity inside the centrifuge pot 21 through the refrigeration surface.
  • the cooling speed is Quick, make The cooling efficiency is high, and the refrigeration component 211 can also be Peltier.
  • the refrigeration component 211 can also realize the heating function, that is, the refrigeration component 211 works in the opposite way to the refrigeration mode, and the refrigeration surface is heated and opposite to the refrigeration surface. surface cooling, thereby achieving the effect of the refrigeration component 211 providing heat.
  • the centrifuge module 100 of the centrifuge device 2 drives the centrifuge cup located in the centrifuge chamber of the centrifuge pot 21 to rotate, and then performs cell processing, and the temperature control module 22 can pass through the refrigeration component 211 attached to the outer wall of the centrifuge pot 21
  • the centrifuge chamber is cooled to ensure that the temperature range of the centrifuge cup for cell processing in the centrifuge chamber is controllable, and the cooling speed is fast, which improves the cooling efficiency; at the same time, the heating surface of the refrigeration component 211 is dissipated through the heat dissipation component 222, which can avoid
  • the temperature of the refrigeration component 211 increases as the refrigeration process proceeds, which avoids the impact of the temperature increase of the refrigeration component 211 on the refrigeration process and ensures the temperature control stability and accuracy of the temperature control module 22 (the temperature control module 22 in this application
  • the temperature control accuracy is high, up to ⁇ 0.3°C; and the temperature adjustable range is accurate, 6°C-37°C), ensuring a constant temperature required
  • the refrigeration component 211 includes a first semiconductor refrigeration piece 2211 with a cooling surface attached to the first side of the centrifuge pot 21;
  • the heat dissipation component 222 includes a refrigeration fan 2221, The heat sink 2222 connected to the heating surface of the first semiconductor refrigeration piece 2211, and the first air duct 2223 connected between the heat sink 2222 and the cooling fan 2221; that is, using the first semiconductor refrigeration piece 2211 is used for refrigeration, which can make its refrigeration and heat dissipation structure simpler than water-cooling pipelines.
  • the refrigeration surface of the first semiconductor refrigeration piece 2211 is first attached to the outer wall of the first side of the centrifuge pot 21 through thermal conductive silicone grease.
  • the outer wall of the first side of the centrifuge pot 21 is provided with a third A groove, by attaching the first semiconductor refrigeration piece 2211 in the first groove, the cold energy can be fully transferred to the inside of the centrifugal cavity during the refrigeration process.
  • the heat sink 2222 is attached to the heating surface of the first semiconductor refrigeration piece 2211 through thermal conductive silicone grease.
  • the heat sink 2222 presses the first semiconductor refrigeration piece 2211 and fixes the heat sink 2222 to the outside of the centrifuge pot 21 through screws. on the wall, and then fix the first air duct 2223 to the heat sink 2222 with screws.
  • the heating surface of the first semiconductor refrigeration chip 2211 discharges heat through the heat sink 2222, the first air duct 2223 and the cooling fan 2221.
  • an end of the first air duct 2223 away from the cooling fan 2221 is provided with a mounting portion 2224 , and the mounting portion 2224 is provided with a heat sink that is adapted to the heat sink 2222 .
  • Positioning groove 2225, the mounting part 2224 is installed on the outer wall of the centrifuge pot 21 through the heat sink 2222 located in the positioning groove 2225. That is to say, the positioning groove 2225 is provided so that the heat sink 2222 can be installed in the positioning groove 2225, which can better protect the heat sink 2222, and also make the installation of the first air duct 2223 more stable, and the cooling fan 2221 The heat dissipation effect of the heat sink 2222 through the first air duct 2223 will also be better.
  • the refrigeration component 211 also includes a second semiconductor refrigeration piece 2212 with a cooling surface attached to the second side of the centrifuge pot 21; the first side and the second side are oppositely arranged; the heat dissipation component 222 also includes The cooling fan 2226, the second air duct 2227, and the heat pipe radiator 2228 connected to the heating surface of the second semiconductor refrigeration piece 2212; one end of the heat pipe radiator 2228 is connected to the cooling fan 2226, and the heat pipe radiator 2228 The other end opposite to the cooling fan 2226 is connected to the air inlet of the second air duct 2227.
  • the refrigeration component 211 can also be attached to multiple sides of the centrifugal pot 21 on approximately the same plane to form an annular distribution, so that A closed refrigeration circle can be formed, which can make the temperature of the center point of the plane drop sharply and accelerate the refrigeration effect at the center point.
  • the first semiconductor refrigeration piece 2211 and the second semiconductor refrigeration piece 2212 are symmetrically installed on the outer wall of the centrifugal pot 21 , so that the refrigeration speed can be further accelerated.
  • the cooling surface of the second semiconductor refrigeration piece 2212 is first attached to the outer side wall of the second side of the centrifuge pot 21 through thermal conductive silicone grease.
  • a second groove is opened on the outer side wall of the second side of the centrifuge pot 21.
  • the heat pipe radiator 2228 is attached to the heating surface of the second semiconductor refrigeration piece 2212 through thermal conductive silicone grease, and the heat pipe radiator 2228 presses the second semiconductor refrigeration piece 2212, and the heat pipe radiator 2228 is fixed to the centrifuge pot 21 through screws.
  • a cooling fan 2226 is provided above the heat pipe radiator 2228, and the second air duct 2227 is fixed on the centrifugal pot 21, and the inlet of the second air duct 2227 The air outlet is located above the heat pipe radiator 2228 and is opposite to the cooling fan 2226. In this way, the cooling fan 2226 can be used to dissipate the heat in the heat pipe radiator through the flowing wind.
  • the temperature control module 22 further includes a temperature sensor 224 installed at the bottom of the centrifuge chamber of the centrifuge pot 21 .
  • the temperature sensor 224 shown is a high-precision temperature sensor 224, and the temperature sensor 224 is inserted into the centrifuge chamber and installed and fixed at the bottom of the centrifuge chamber to detect the air temperature inside the centrifuge chamber in real time during cell processing.
  • the temperature sensor 224 can be fixed with thermally conductive glue, and the temperature in the centrifuge pot 21 monitored in real time by the temperature sensor 224 can be fed back to the control panel of the cell processing equipment, and then the refrigeration component 211 (such as the first The current of the semiconductor refrigeration piece 2211 and the second semiconductor refrigeration piece 2212) is controlled to maintain the temperature in the centrifuge pot 21 within a preset range.
  • the refrigeration component 211 such as the first The current of the semiconductor refrigeration piece 2211 and the second semiconductor refrigeration piece 2212
  • the bottom of the centrifuge pot 21 is provided with a drain port (not shown), and the centrifuge device 2 further includes a centrifuge chamber provided at the bottom of the centrifuge pot 21
  • the liquid detection sensor 25 (wherein the liquid detection sensor 25 is fixed by sealant), and the drain joint 23 connected to the drain port. Understandably, the liquid detection sensor 25 can monitor the remaining liquid inside the centrifuge chamber. For example, when the liquid detection sensor 25 detects that a certain amount of liquid appears at the bottom of the centrifuge chamber, the controller will trigger a prompt based on the detection signal received from the liquid detection sensor 25 , furthermore, the controller can control the drain connector 23 to open, and then discharge the liquid through the drain port and the drain connector 23 .
  • the drain joint 23 can be connected to an external drainage pipe to sequentially drain the liquid (such as condensed water) in the centrifugal chamber through the drainage port, the drainage interface and the drainage pipe to prevent liquid accumulation or leakage.
  • the edge of the centrifuge chamber of the centrifuge pot 21 is provided with a connecting flange 212; the centrifugal device 2 also includes a connecting flange 212 that is fitted to the centrifugal chamber edge.
  • the connected sealing ring 24, and the flip cover 26 rotatably connected to the housing 5, the flip cover 26 is provided at the edge of the centrifuge chamber and is used to cooperate with the seal ring 24 to cover or open the centrifuge chamber. .
  • the sealing ring 24 is bonded and fixed on the connection ring on the top of the centrifuge pot 21 so as to cooperate with the flip cover 26 of the centrifuge device 2 to seal the centrifuge chamber, ensuring the safety and airtightness of the cell processing process. It can also be used Avoid air-conditioning leakage.
  • the flip cover 26 can be opened. After the liquid in sample 4 is consumed (after all the liquid is injected into the centrifuge cup for cell processing), operations such as intubation and centrifuge cup replacement need to be performed. You can The flip cover 26 is then closed until it fits the sealing ring 24 to seal the centrifuge chamber, thereby providing safety protection and maintaining the temperature in the centrifuge chamber.
  • the cell processing equipment further includes a controller (not shown) installed in the housing 5 for scanning and identifying the corresponding representation of the sample 4.
  • the code scanning device 6, the display device 7 for performing touch operations or displaying parameters, the weighing component 12, the centrifugal device 2, the liquid circuit module 3, the code scanning device 6 and the The display devices 7 are all connected to the controller.
  • the display device 7 is used to set, modify and monitor relevant parameters during the cell processing process by touching the human-computer interaction display interface; the display device 7 can be rotatably connected (for example, through a hinge to connect the shell 5 to the shell 5) for convenience.
  • the rotation angle of the display device 7 can be adjusted according to the operator's visual angle requirements, thereby improving the user experience; understandably, the shape and size of the display device 7 can be set according to needs.
  • the code scanning device 6 is used to scan the identification code on the sample 4 and identify it, and then transmit the identified data information to the controller for use.
  • the size, shape, etc. of the code scanning device 6 can also be set according to requirements.
  • the pipeline assembly 31 includes a pinch valve 311 for controlling the opening and closing of the pipeline in the pipeline assembly 31, and a pinch valve 311 for monitoring the flow in the pipeline in real time.
  • the bubble sensor 312 is used to determine whether there are bubbles mixed in the liquid in the sample 4
  • the pressure sensor 313 is used to monitor the pressure of the liquid in the pipeline in real time
  • the peristalsis sensor is used to drive the flow of the liquid in the pipeline during operation.
  • the pinch valve 311, the bubble sensor 312, the pressure sensor 313 and the peristaltic pump 314 are installed on the front of the liquid circuit panel 32
  • the stepper motor 315 is installed on the back of the liquid circuit panel 32 at a position opposite to the peristaltic pump 314, and the stepper motor 315 is located on the housing.
  • the pinch valve 311, the bubble sensor 312, the pressure sensor 313 and the peristaltic pump 314 are installed on the front of the liquid circuit panel 32 and fixed with screws
  • the stepper motor 315 is installed on the back of the liquid circuit panel 32.
  • the peristaltic pump 314 is installed above the stepper motor 315 and located on the front of the liquid path panel 32, and is fixed with screws.
  • the pipeline assembly 31 also includes pipelines connected between the components.
  • the stepper motor 315 drives the peristaltic pump 314 to rotate, which serves as the driving force for the flow of liquid in the sample 4 and drives the liquid to flow.
  • the pinch valve 311 (can be set to one or more as required, and can be driven by an electromagnetic switch) plays a switching role, controlling the on and off of the liquid in the pipeline used to transport the liquid in the sample 4 and changing the flow path.
  • the bubble sensor 312 (one or more can be set according to requirements) is used to monitor in real time whether the fluid in the pipeline is mixed with bubbles, and use it as a criterion for determining whether the liquid in sample 4 is used up.
  • the pressure sensor 313 (can be set to one or more according to requirements) monitors the pressure of the liquid in the pipeline in real time. If the pressure exceeds the preset pressure threshold, the controller will send a feedback signal after receiving the pressure signal from the pressure sensor 313 , the automatic control pipeline component 31 adjusts itself or notifies the corresponding operator of the cell processing equipment to make adjustments.
  • the pipeline assembly 31 in this application is open-type arranged on the liquid circuit panel 32.
  • the liquid circuit panel 32 can be installed on the housing 5 at an angle of 125°, which is convenient for operation, installation and disassembly. At the same time, the liquid circuit panel 32 is equipped with The pressure sensor 313 monitors the pressure of the liquid line to prevent pipeline blockage; a bubble sensor 312 is also installed on the liquid line panel 32 to monitor the bubbles in the pipeline and is used to determine whether the liquid in sample 4 has been used up, making the use process more convenient. convenient.
  • the relevant parameters can be set through the touch human-computer interaction display interface of the display device 7, and the code scanning device 6 is used to scan and identify the identification code on the sample 4, and then the data information is obtained and sent to the controller to complete the data information. of entry.
  • the sample 4 is hung on the hook 123 of the corresponding weighing device 1.
  • the pipeline is connected to the pinch valve 311, pressure sensor 313 and bubble sensor 312 installed on the liquid circuit panel 32.
  • the cell processing equipment of this application is small in size, occupies a small area, and is easy to install and transport; it has an accurate temperature adjustable range (4°C-40°C) and high temperature control accuracy ( ⁇ 0.3°C); at the same time, the cell processing equipment has Low power consumption, long life and low noise.

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Abstract

本申请属于细胞处理技术领域，特别是涉及一种细胞处理设备。该细胞处理设备的称重装置设置至少一组称重组件，通过上述称重组件可以同时对一个或者多个悬挂在挂钩上的样品进行称重检测，提升了称重效率，且每个挂钩上悬挂的样品均通过对应配置的称重传感器进行称重，各称重结果不会相互干涉；同时，本申请中的每一组称重组件均可实现单组称重组件的拆卸和安装，便于检修及维护。且本申请通过专门为离心杯配置的离心模组安装离心杯并带动离心杯旋转，使得离心杯的安装更为便捷和牢固，避免了离心杯脱落的情况出现，保证了细胞处理过程的安全性。本申请结构简单，减小了设备体积，便于检修。

Description

细胞处理设备
本申请要求于2022年7月22日提交、申请号为202210870017.7，发明名称为“细胞处理设备”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本申请属于细胞处理技术领域，特别是涉及一种细胞处理设备。
背景技术
目前，通常通过细胞处理设备进行细胞处理，比如细胞分离或混匀等。现有技术中的细胞处理设备通常功能单一，设备体积大。发明人发现，在细胞处理设备进行细胞处理的过程中，细胞处理设备往往只能通过称重装置每次对一组待处理样品进行称重，因此称重效率低；并且，称重装置的体积较大，在发生故障时，排查故障的流程繁琐，不利于检修。
申请内容
本申请针对现有技术中细胞处理设备功能单一且称重效率低等技术问题，提供了一种细胞处理设备。
鉴于以上技术问题，本申请实施例提供一种细胞处理设备，包括：
称重装置，包括支撑组件和至少一组称重组件，所述支撑组件包括用于支撑所述称重组件的支架；每一组所述称重组件均包括安装在所述支架上的安装支座、安装在所述安装支座上的称重传感器，以及可拆卸安装在所述称重传感器上以悬挂样品的挂钩；
离心装置，包括具有离心腔的离心锅、设置在所述离心腔内的离心杯、连接所述离心锅并用于带动所述离心杯旋转的离心模组；
液路模组，包括用于将所述样品内的液体输送到所述离心杯内进行细胞处理的管路组件，以及用于安装和控制所述管路组件中液体流动的液路面板；
壳体，所述离心锅、所述液路面板和所述支架均安装在所述壳体上。
本申请的细胞处理设备中，称重装置设置至少一组称重组件，通过上述称重组件可以同时对一个或者多个悬挂在挂钩上的样品进行称重检测，提升了称重效率，同时，称重组件上设置有用于悬挂样品的挂钩，方便挂取样品，且使得被悬挂的样品中的液体流动不被阻碍；并且，在同时对多个样品进行称重时，由于每个挂钩上悬挂的样品均通过与其对应配置的称重传感器进行称重，因此，各样品的称重结果将互相独立而不会相互干涉；同时，本申请中的每一组称重组件均通过安装支座安装在支架上，因此可以实现单组称重组件的拆卸和安装，便于检修及维护。并且，本申请通过专门为离心杯配置的离心模组安装离心杯并带动离心杯旋转，使得离心杯的安装更为便捷和牢固，避免了离心杯脱落的情况出现，保证了细胞处理过程的安全性。本申请的细胞处理设备结构简单，减小了设备体积；且各部分均独立可拆卸地安装在壳体上，在发生故障时，简化了排查故障的流程，便于检修。
本申请的一个或多个实施例的细节在下面的附图和描述中提出。本申请的其它特征和优点将从说明书、附图以及权利要求书变得明显。
附图说明
下面结合附图和实施例对本申请进一步说明。
图1是本申请一实施例提供的细胞处理设备的结构示意图。
图2是本申请一实施例提供的细胞处理设备的称重装置的爆炸结构示意图。
图3是本申请一实施例提供的细胞处理设备的称重组件的爆炸结构示意图。
图4是本申请一实施例提供的细胞处理设备的挂钩的结构示意图。
图5是本申请另一实施例提供的细胞处理设备的挂钩的结构示意图。
图6是本申请一实施例提供的细胞处理设备的离心模组的装配结构示意图。
图7是本申请一实施例提供的细胞处理设备的离心模组的爆炸结构示意图。
图8是本申请一实施例提供的细胞处理设备的离心模组和温控模组的爆炸结构示意图。
图9是本申请一实施例提供的细胞处理设备的离心模组和温控模组的装配结构示意图。
图10是本申请一实施例提供的细胞处理设备的液路模组的结构示意图。
说明书中的附图标记如下：
1、称重装置；11、支撑组件；111、支架；1111、安装板；1112、安装孔；1113、支
撑杆；112、前壳；113、后壳；12、称重组件；121、安装支座；1211、容置槽；1212、固定孔；1213、翻边；122、称重传感器；123、挂钩；1231、连接螺纹；1232、折弯段；1233、侧偏段；1234、挂接段；12341、连接部；1235、开口；a、预设侧偏角度；L1、挂接段的中心轴线；124、前盖；125、后盖；
2、离心装置；21、离心锅；212、连接翻边；22、温控模组；221、制冷组件；2211、
第一半导体制冷片；2212、第二半导体制冷片；222、散热组件；2221、制冷风扇；2222、散热片；2223、第一风管；2224、安装部；2225、定位槽；2226、散热风扇；2227、第二风管；2228、热管散热器；223、保温层；224、温度传感器；23、排液接头；24、密封圈；25、液体检测传感器；26、翻盖；
100、离心模组；101、旋转轴；1011、定位台阶；1012、销钉；1013、销孔；102、
轴承组件；1021、轴承座；1022、轴承；103、联轴器；104、驱动电机；105、制动器；1051、过孔；106、旋转安装座；107、不规则轮；108、电机安装座；1081、电机容置空间；109、固定板；
3、液路模组；31、管路组件；311、夹管阀；312、气泡传感器；313、压力传感器；
314、蠕动泵；315、步进电机；32、液路面板；
4、样品；5、壳体；51、启动按钮；6、扫码装置；7、显示装置。
具体实施方式
为了使本申请所解决的技术问题、技术方案及有益效果更加清楚明白，以下结合附图及实施例，对本申请进行进一步的详细说明。应当理解，此处所描述的具体实施例仅用以解释本申请，并不用于限定本申请。
需要理解的是，术语“上”、“下”、“左”、“右”、“前”、“后”、“中部”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是是为了便于描述本申请和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为本申请的限制。
如图1和图2所示，本申请一实施例提供了一种细胞处理设备，包括：
称重装置1，包括支撑组件11和至少一组称重组件12，所述支撑组件11包括用于支撑所述称重组件12的支架111；每一组所述称重组件12均包括安装在所述支架111上的安装支座121、安装在所述安装支座121上的称重传感器122，以及可拆卸安装在所述称重传感器122上以悬挂样品4(比如用于承装液体的液袋)的挂钩123；其中，所述称重传感器122可以为通过压力信号确定重量的压力传感件。所述支架111以及所述安装支座121均可以为钣金件，以使得支架111更为牢固，可以实现更好地承重，同时通过不易变形的钣金件的安装支座121可以更好地保护称重传感器122等。所述支架111的形状和尺寸可以根据需求进行设定，只要使得称重组件12可以稳定安装在支架111上，且尽可能减小整个称重装置1的体积即可。在本申请的上述实施例中，称重装置1中设置一组或者 多组相同结构的称重组件12(称重组件12的数量可以根据需求进行设定)，每一组称重组件12均通过安装支座121安装在支架111上，因此可以实现单组称重组件12的拆卸和安装，且在单组称重组件12损坏时，也不会影响其他组称重组件12的使用，同时，由于每一组称重组件12的结构都相同，因此便于检修及维护。可理解地，所述挂钩123也采用可拆卸连接在称重传感器122上的结构设计，因此，便于挂钩123的拆卸和安装的同时，还可以提升排除故障的效率。在一实施例中，所述称重传感器122上设有螺纹孔(图未示)，所述挂钩123上设有连接螺纹1231，所述挂钩123通过所述连接螺纹1231与所述螺纹孔螺纹连接。也即，在本实施例中，通过连接螺纹1231与螺纹孔的螺纹连接实现挂钩123与称重传感器122之间的可拆卸连接。
进一步地，如图2和图3所示，所述安装支座121上设有与所述称重传感器122适配的容置槽1211，所述容置槽1211的内壁上设有固定孔1212，所述称重传感器122通过穿过所述固定孔1212的螺钉被固定安装在所述容置槽1211中。也即，安装支座121可以为折弯形成所述容置槽1211的折弯板，称重传感器122安装在容置槽1211中，可以被安装支座121保护不易受到损坏。由于称重传感器122的下端需要和挂钩123连接，因此，本实施例中的固定孔1212设置在容置槽1211的顶部内壁上，以使得称重传感器122的顶部与安装支座121固定而不易从所述容置槽1211中脱出。可选地，所述安装支座121包括设置在所述容置槽1211边缘的翻边1213，所述安装支座121通过所述翻边1213安装在所述支架111上。具体地，安装支座121可通过上述翻边1213固定在安装板1111上，且两者的连接方式可以为螺钉连接、卡扣连接等可拆卸连接方式，以便于可以从安装板1111上将安装支座121以及整个称重组件12拆离。
离心装置2，包括具有离心腔的离心锅21、设置在所述离心腔内的离心杯(图未示)、连接所述离心锅21并用于带动所述离心杯旋转的离心模组100；其中，离心装置2的离心模组100带动位于离心锅21的离心腔内的离心杯旋转，以对离心杯中注入的液体进行细胞处理。在该实施例中，通过专门为离心杯配置的离心模组100安装离心杯并带动离心杯旋转，使得离心杯的安装更为便捷和牢固，避免了离心杯脱落的情况出现，保证了细胞处理过程的安全性。
液路模组3，包括用于将所述样品4内的液体输送到所述离心杯内进行细胞处理的管路组件31，以及用于安装和控制所述管路组件31中液体流动的液路面板32；也即，上述液路模组3中，如图10所示，管路组件31安装在液路面板32上，且通过在液路面板32上操作控制上述管路组件31，可以使得样品4中的液体可以顺利同管路组件31流入离心杯中进行细胞处理。
壳体5，所述离心锅21、所述液路面板32和所述支架111均安装在所述壳体5上。可理解地，壳体5的形状可以根据需求进行设定，并不限定为如图1中所示，壳体5包括支撑骨架、安装在支撑骨架底部的底板、安装在支撑骨架四周的侧板、安装在支撑骨架顶部的顶板。上述底板、顶板和侧板可以通过螺钉连接、卡扣连接或焊接等方式组装在一起，其中的底板、顶板和侧板均可以与支撑骨架设置为可拆卸连接，以便于从被拆卸的位置对壳体5内部的元器件进行安装维修(如插管、更换离心杯等)。
本申请的细胞处理设备的称重装置1设置至少一组称重组件12，通过上述称重组件12可以同时对一个或者多个悬挂在挂钩123上的样品4进行称重检测，提升了称重效率，同时，称重组件12上设置有用于悬挂样品4的挂钩123，方便挂取样品4，且使得被悬挂的样品4中的液体流动不被阻碍；并且，在同时对多个样品4进行称重时，由于每个挂钩123上悬挂的样品4均通过与其对应配置的称重传感器122进行称重，因此，各样品4的称重结果将互相独立而不会相互干涉；同时，本申请中的每一组称重组件12均通过安装支座121安装在支架111上，因此可以实现单组称重组件12的拆卸和安装，便于检修及维护。并且，本申请通过专门为离心杯配置的离心模组100安装离心杯并带动离心杯旋转， 使得离心杯的安装更为便捷和牢固，避免了离心杯脱落的情况出现，保证了细胞处理过程的安全性。本申请的细胞处理设备结构简单，减小了设备体积；且各部分均独立可拆卸地安装在壳体5上，在发生故障时，简化了排查故障的流程，便于检修。
在一实施例中，如图2和图3所示，所述称重组件12为至少两组；所述支架111包括间隔(优选为均匀间隔)设置有安装孔1112的安装板1111，所述安装孔1112的数量与所述称重组件12的数量一致；所述安装支座121将所述称重传感器122安装在所述安装板1111上与所述安装孔1112相对的位置上，所述挂钩123穿过所述安装孔1112连接所述称重传感器122。也即，在该实施例中，每一个安装孔1112均用于安装一组称重组件12，可理解地，上述安装孔1112亦可以设计为槽，只要能使得挂钩123穿过以与称重传感器122连接，且保证安装支座121在安装板1111上的安装稳固可靠即可。进一步地，所述支架111还包括连接所述安装板1111的支撑杆1113，所述支撑组件11还包括前壳112和后壳113，所述前壳112和所述后壳113之间围成用于安装所述支撑杆1113的安装空间。也即，支撑杆1113用于支撑安装板1111以及安装在安装板1111上的所有称重组件12，以保持称重时的称重组件12的稳定。而前壳112和后壳113用于对支撑杆1113进行保护。在一实施例中，所述称重组件12还包括前盖124和后盖125，所述前盖124和所述后盖125之间围成用于容纳所述安装板1111、所述安装支座121以及所述称重传感器122的容纳空间。也即，在称重组件12安装在安装板1111上之后，前盖124和后盖125将安装板1111以及称重组件12中的称重传感器122进一步保护在容纳空间中，避免其受到损坏。
进一步地，如图4和图5所示，所述挂钩123包括折弯段1232、侧偏段1233以及连接所述称重传感器122的的挂接段1234(可理解地，上述连接螺纹1231即可为设置在所述挂接段1234上的外螺纹)，所述折弯段1232的相对两端分别连接所述挂接段1234与所述侧偏段1233；所述折弯段1232与所述侧偏段1233围成具有开口1235的勾持环；所述折弯段1232与所述挂接段的中心轴线L1均位于第一预设基准平面上，所述侧偏段1233的中心轴线与所述第一预设基准平面呈预设侧偏角度a设置。在一实施例中，所述折弯段1232、所述侧偏段1233以及所述挂接段1234为一体成型结构，如此可以便于简化挂钩123的制造工艺，提升制备效率，降低制造成本。在一些实施例中，所述折弯段1232、所述侧偏段1233以及所述挂接段1234之间可拆卸连接亦可，如此便于储存和运输，在其中一部分损坏需要更换时，可以仅更换该部分即可。可理解地，所述挂钩123的材质可以优选为金属，比如不锈钢。可理解地，折弯段1232的折弯形状及折弯角度等可以根据需求设定，在该实施例中，折弯段1232与所述挂接段的中心轴线L1均位于同一平面中(也即两者均位于第一预设基准平面中，进一步地，在挂钩123可拆卸连接在称重传感器122上时，该第一预设基准平面与水平面垂直)，在一些实施例中，折弯段1232的中心轴线亦可以部分位于第一预设基准平面中，另一部分则折弯延伸至第一预设基准平面外亦可。可理解地，勾持环的形状随着侧偏段1233与折弯段1232的形状变化，在本申请中并不加以限定，在需要将样品4悬挂到勾持环中去时，样品4将从勾持环的开口1235处挂入。具体地，所述侧偏段1233与折弯段1232的连接点位于第一预设基准平面上，从该连接点开始，侧偏段1233朝向远离第一预设基准平面的方向侧向偏移，可理解地，在从连接点朝向远离所述折弯段1232的一端的方向上，侧偏段1233与第一预设基准平面之间的距离逐渐增大。需要明确的是，在本申请中，侧偏段1233并不一定是一条直线，其可以为曲线(侧偏段1233的中心轴线可以根据其弯曲方向等拟合为一条直线)，只要其朝向远离第一预设基准平面的方向偏离且偏离角度达到预设偏离角度即可。
本实施例中，样品4(图未示)可以从勾持环的开口1235位置被正向挂置(样品4较宽的一面朝向细胞处理装置的操作台)入勾持环内，并且，由于侧偏段1233的中心轴线与第一预设基准平面呈预设侧偏角度a设置，因此，在样品4依靠自身重力沿侧偏段1233 下落至勾持环底部时，样品4将会自然被侧向悬挂(样品4较窄的一面朝向操作台)在挂钩123上，进而减少在细胞处理设备的支撑组件11上排列的样品4所占的横向宽度，进而可以在同一个支撑组件11上可以悬挂更多的样品4，提升了操作效率；且在通过本申请挂钩123悬挂样品4的过程中，操作人员可以直接从正向挂置样品4而得到侧向排布的样品4，省时省力，大大提升了用户操作体验。
进一步地，所述预设侧偏角度a为30-60度。作为优选，所述预设侧偏角度a为45度。所述预设侧偏角度a设置为属于上述角度范围，可以使得操作人员可以从操作台所在的正向进行挂置样品4的操作，提升了操作便捷性，同时亦可使得正向进行挂置后的样品4依靠自身重力沿着勾持环中侧偏段1233下落进而实现侧向排布，进而减少在细胞处理设备的支撑组件11上排列的样品4所占的横向宽度，使得同一个支撑组件11上可以悬挂更多的样品4，提升了操作效率。
在一实施例中，如图4和图5所示，所述挂接段的中心轴线L1穿过所述折弯段1232和所述侧偏段1233之间的连接点；第一相对距离大于或等于第二相对距离；其中，所述第一相对距离是指所述连接点与第二预设基准平面之间的相对距离；所述第二预设基准平面垂直于所述挂接段1234且穿过所述挂接段1234上的任意一点；所述第二相对距离是指所述折弯段1232与所述侧偏段1233上除所述连接点之外的其他点与所述第二预设基准平面之间的相对距离。可理解地，在挂钩123安装到细胞处理设备上之后，挂接段1234及其所处的第一预设基准平面均垂直于水平面，由于所述第二预设基准平面垂直于所述挂接段1234，因此，此时第二预设基准平面即为与水平面平行的平面，且第二基准平面穿过所述挂接段1234上的任意一点，比如，第二基准平面穿过挂接段1234与折弯段1232的邻接点，此时，由于第一相对距离大于或等于第二相对距离，因此，所述折弯段1232和所述侧偏段1233之间的连接点为整个挂钩123当前所处安装状态的位置最低点，因此，从勾持环的开口1235位置被挂置到挂钩123上的样品4，在自身重力作用下自然下落之后，会落入到所述折弯段1232和所述侧偏段1233之间的连接点位置，且该连接点位于挂接段的中心轴线L1正下方，因此，样品4在滑落到连接点位置上之后，挂钩123的挂接段1234依旧会保持与水平面垂直，而不会在样品4的重力作用下偏移或者晃动，进而保证了细胞处理设备的稳定性。可选地，所述折弯段1232包括与所述挂接段的中心轴线L1平行的直线段。上述设置可以使得挂钩123的结构更为稳固可靠。
在一实施例中，如图6和图7所示，所述离心模组100包括旋转轴101、轴承组件102、联轴器103、用于驱动所述旋转轴101旋转的驱动电机104、用于对所述旋转轴101进行制动的制动器105以及位于所述离心腔内且用于安装所述离心杯的旋转安装座106；所述联轴器103连接在所述驱动电机104的输出端与所述旋转轴101之间；所述旋转轴101远离所述联轴器103的一端穿过所述轴承组件102连接所述旋转安装座106；所述制动器105连接所述轴承组件102。其中，旋转安装座106通过销钉1012安装在旋转轴101上，具体地，旋转轴101远离所述联轴器103的一端开设有销孔1013，而旋转安装座106卡入穿过销孔1013的销钉1012后，再通过螺钉固定在旋转轴101上；进一步地，旋转安装座106上设有第一卡接部，离心杯上设有第二卡接部，离心杯通过所述第一卡接部和第二卡接部的卡接与旋转安装座106连接，进一步地，卡接之后的离心杯和旋转安装座106还可以进一步通过螺钉组件固定连接以使两者之间的连接稳固，通过设置所述旋转安装座106，可以使得改离心模组100兼容多种规格的离心杯。进一步地，所述驱动电机104为无刷直流电机，以提升离心模组100的电机使用寿命并降低噪音。进一步地，所述联轴器103为膜片式联轴器。由于膜片式联轴器无背隙，因此使用膜片式联轴器可以进一步提升离心模组100的传动精度。可理解地，制动器105在断电时通过制动盘对旋转轴101进行制动；而制动器105在通电情况下释放制动盘解除旋转轴101的制动状态，此时驱动电机104可以运转进而通过联轴器103带动旋转轴101旋转，旋转轴101则带动安装在旋转安装座106 上的离心杯转动，以实现对离心杯中的样品4(比如血液)成分进行分离、混匀、制剂及清洗等细胞处理。
本实施例中，旋转轴101转动安装在轴承组件102上，以限制旋转轴101径向跳动范围，减小离心模组100带动离心杯转动时的径向跳动；同时，旋转轴101通过联轴器103连接旋转轴101和驱动电机104，且通过制动器105和驱动电机104分别对旋转轴101进行制动和驱动，其结构简单，提升了传动精度；并且本申请中通过安装在旋转轴101上的旋转安装座106专门适配安装离心杯，使得离心杯的安装更为便捷和牢固，避免了旋转轴101通过旋转安装座106带动离心杯转动时，离心杯脱落的情况出现，保证了细胞处理过程的安全性，以及细胞处理的质量可靠，效果更佳。
进一步地，如图7所示，所述轴承组件102包括具有安装通孔的轴承座1021以及安装在所述安装通孔的内侧壁上的两个轴承1022；所述旋转轴101穿过两个所述轴承1022安装在所述轴承座1021上；所述制动器105安装在所述轴承座1021上。所述制动器105上穿设有过孔1051，所述旋转轴101穿过所述过孔1051之后通过两个所述轴承1022与所述轴承座1021转动连接，进一步地，制动器105通过螺钉固定安装在轴承座1021上，而两个轴承1022的外圈过盈配合安装在在轴承座1021中安装通孔的内侧壁上，可选地，所述安装通孔的内侧壁上凹陷形成平行设置的两个环形凹槽(图未示)，两个所述轴承1022分别卡嵌在两个所述环形凹槽中，旋转轴101在穿过制动器105上的过孔1051之后，再从两个轴承1022中穿过，以通过两个轴承1022与轴承座1021转动连接；可选地，所述旋转轴101上设有定位台阶1011，两个所述轴承1022安装在所述定位台阶1011上，以将轴承1022内圈稳定地固定在定位台阶1011上。本实施例中使用双轴承1022的轴承组件102对旋转轴101进行限位，在最大程度上限制了旋转轴101的径向跳动范围。
进一步地，如图6和图7所示，所述离心模组100还包括安装在所述旋转轴101上的不规则轮107，所述制动器105上设有与所述不规则轮107适配的卡槽(图未示)。可理解地，所述不规则轮107的形状可以根据需求设定，比如，不规则轮107可以为方轮，此时，卡槽将设置为与方轮适配的方槽。在制动器105进行制动之前，不规则轮107与卡槽脱离，此时方槽不会对与方轮连接的旋转轴101进行制动；而在制动器105制动之后，首先通过制动盘对旋转轴101进行制动，之后，旋转轴101的转动速度降低，方轮也会随着旋转轴101的轴向移动卡嵌至卡槽中，进而使得旋转轴101迅速停止运动。
在一实施例中，如图6和图7所示，所述离心模组100还包括具有电机容置空间1081的电机安装座108，所述驱动电机104安装在所述电机容置空间1081中；所述轴承组件102安装在所述电机安装座108上。具体地，本实施例通过电机安装座108上开设的沉台所形成的电机容置空间1081安装放置驱动电机104，电机容置空间1081的内侧壁上设有中心轴孔，电机的输出端(也即输出轴)穿过该中心轴孔与联轴器103连接，之后，驱动电机104将被固定安装(比如通过螺钉连接)在该沉台内。进一步地，离心模组100还包括用于连接外部构件的固定板109，所述电机安装座108远离所述轴承座1021的一端安装在所述固定板109上。也即，电机安装座108上的沉台开口端(电机容置空间1081的开口端)与固定板109连接固定，进而可以同该固定板109将整个离心模组100固定在外部构件上，比如，固定在细胞处理装置的离心锅21的离心腔底部，以在离心腔内通过离心模组100带动离心杯进行细胞处理操作。
在一实施例中，如图8和图9所示，所述离心装置2还包括用于控制所述离心腔温度的温控模组22，所述温控模组22包括用于对所述离心腔进行制冷的制冷组件211，以及用于对所述制冷组件211进行散热的散热组件222；所述制冷组件211的制冷面贴附在所述离心锅21的外侧壁上，所述制冷组件211的加热面连接所述散热组件222。其中，制冷组件211可以为制冷面贴附在离心锅21的内侧壁上的至少一片半导体制冷片，通电之后的半导体制冷片可以通过制冷面对离心锅21内部的离心腔进行制冷，其制冷速度快，制 冷效率高，所述制冷组件211还可以为珀尔帖，所述制冷组件211也可以实现加热功能，即将所述制冷组件211实现与制冷方式相反工作方式，制冷面进行加热，与制冷面相对的面制冷，从而达到制冷组件211提供热量的效果。其中，离心装置2的离心模组100带动位于离心锅21的离心腔内的离心杯旋转，进而进行细胞处理，而温控模组22可以通过贴附在离心锅21外侧壁上的制冷组件211对离心腔进行制冷，进而保证离心腔内进行细胞处理的离心杯的温度范围可控，其制冷速度快，提升了制冷效率；同时通过散热组件222对制冷组件211的加热面进行散热，可以避免制冷组件211的温度随着制冷过程进行而提升，避免了制冷组件211的温度提升对制冷过程的影响，保证了温控模组22的温度控制稳定性和精度(本申请中温控模组22的温度控制精度高，可达±0.3℃；且温度可调范围精确，6℃-37℃)，保证了细胞处理所需的温度恒定；且本申请的温控模组22结构简单，体积小，降低了设备成本。
进一步地，如图8和图9所示，所述制冷组件211包括制冷面贴附在所述离心锅21的第一侧的第一半导体制冷片2211；所述散热组件222包括制冷风扇2221、连接所述第一半导体制冷片2211的制热面的散热片2222，以及连接在所述散热片2222和所述制冷风扇2221之间的第一风管2223；也即，使用第一半导体制冷片2211进行制冷，可以使得其制冷和散热结构相比于水冷管路更简便。在本实施例中，第一半导体制冷片2211的制冷面首先通过导热硅脂贴合于离心锅21第一侧的外侧壁上，具体地，离心锅21第一侧的外侧壁上开设有第一凹槽，将第一半导体制冷片2211贴附在该第一凹槽内即可在其制冷过程中将冷量充分传递到离心腔内部。可理解地，散热片2222通过导热硅脂贴附在第一半导体制冷片2211的制热面，散热片2222压紧第一半导体制冷片2211并通过螺钉将散热片2222固定在离心锅21的外侧壁上，之后将第一风管2223用螺钉固定在散热片2222上，最后将制冷风扇2221安装在第一风管2223远离所述散热片2222的一端，再用螺钉将其固定，如此，第一半导体制冷片2211通电后，第一半导体制冷片2211的加热面通过散热片2222、第一风管2223及制冷风扇2221将热量排出。
进一步地，如图8和图9所示，所述第一风管2223远离所述制冷风扇2221的一端设有安装部2224，所述安装部2224上设有与所述散热片2222适配的定位槽2225，所述安装部2224通过位于所述定位槽2225中的所述散热片2222安装在所述离心锅21的外侧壁上。也即，设置定位槽2225，以将散热片2222安装再定位槽2225内，可以对散热片2222进行更好的保护，同时也可以使得第一风管2223的安装更为稳固，且制冷风扇2221通过第一风管2223对散热片2222进行散热的效果也将更好。所述制冷组件211还包括制冷面贴附在所述离心锅21的第二侧的第二半导体制冷片2212；所述第一侧和所述第二侧相对设置；所述散热组件222还包括散热风扇2226、第二风管2227以及连接所述第二半导体制冷片2212的制热面的热管散热器2228；所述热管散热器2228的一端连接所述散热风扇2226，所述热管散热器2228与所述散热风扇2226相对的另一端连接第二风管2227的进风口，所述制冷组件211还可以贴附在所述离心锅21的近似同一平面的多侧，形成环型状分布，这样可以形成封闭的制冷圈，该制冷圈能够让该平面的中心点的温度急剧下降，加快中心点位置的制冷效果。
也即，该实施例中，如图8和图9所示，第一半导体制冷片2211和第二半导体制冷片2212对称安装再离心锅21的外侧壁上，如此，可以进一步加快制冷速度。其中，第二半导体制冷片2212的制冷面首先通过导热硅脂贴合于离心锅21第二侧的外侧壁上，具体地，离心锅21第二侧的外侧壁上开设有第二凹槽，将第二半导体制冷片2212贴附在该第二凹槽内即可在其制冷过程中将冷量充分传递到离心腔内部。而热管散热器2228通过导热硅脂贴合第二半导体制冷片2212的制热面，并且，热管散热器2228压紧第二半导体制冷片2212，通过螺钉将热管散热器2228固定在离心锅21的外侧壁上；热管散热器2228上方设置有散热风扇2226，而第二风管2227固定在离心锅21上，且第二风管2227的进 风口位于热管散热器2228上方且与散热风扇2226相对设置，如此，可以利用散热风扇2226将热管器散热器中的热量通过流动的风挟裹排出。
在一实施例中，如图8和图9所示，所述的温控模组22还包括安装在所述离心锅21的离心腔底部的温度传感器224。其中，所示温度传感器224为高精度温度传感器224，且温度传感器224插入离心腔内部并安装固定在离心腔底部，用于在进行细胞处理的过程中实时检测离心腔内部空气温度。可理解地，温度传感器224可以通过导热胶固定，温度传感器224实时监控到的离心锅21内温度可以反馈给细胞处理设备的控制板，进而通过控制板根据该温度控制制冷组件211(比如第一半导体制冷片2211和第二半导体制冷片2212)的电流，进而控制离心锅21内温度保持在预设范围内。
在一实施例中，如图8和图9所示，所述离心锅21底部设有排液口(图未示)，所述离心装置2还包括设置在所述离心锅21的离心腔底部的液体检测传感器25(其中，液体检测传感器25通过密封胶固定)，以及连通所述排液口的排液接头23。可理解地，液体检测传感器25可以监测离心腔内部残留的液体，比如，液体检测传感器25监测到离心腔底部出现一定量液体时，控制器会根据接收到的液体检测传感器25的检测信号触发提示，进而，控制器可以控制排液接头23打开，进而通过排液口和排液接头23排出该液体。进一步地，排液接头23可以外接引流管，以顺次通过排液口、排液接口以及引流管排出离心腔内的液体(比如冷凝水)，以防止液体堆积或漏液。
在一实施例中，如图1、图8和图9所示，所述离心锅21的离心腔边缘设有连接翻边212；所述离心装置2还包括与所述连接翻边212贴合连接的密封圈24，以及转动连接在所述壳体5上的翻盖26，所述翻盖26设置在所述离心腔边缘且用于与所述密封圈24配合以盖合或打开所述离心腔。也即，密封圈24粘接固定在离心锅21顶部的连接翻遍上，以便于与离心装置2的翻盖26配合对离心腔进行密封，保证了细胞处理过程的安全性和密闭性，也可以避免冷气外泄。在细胞处理设备开始工作之前，可以将翻盖26打开，而在样品4中液体被消耗完毕(全部注入离心杯中进行细胞处理之后)，此时需要进行如插管、更换离心杯等操作，可以再将翻盖26关闭到与密封圈24贴合以密封离心腔，即可起到安全保护及保持离心腔内温度的作用。
在一实施例中，如图1所示，所述细胞处理设备还包括安装在所述壳体5内的控制器(图未示)、用于对所述样品4对应的表示进行扫码识别的扫码装置6、用于进行触控操作或显示参数的显示装置7，所述称重组件12、所述离心装置2、所述液路模组3、所述扫码装置6以及所述显示装置7均连接所述控制器。其中，显示装置7通过触摸人机交互显示界面进行细胞处理过程中的相关参数设置、修改和监控；显示装置7可转动连接(比如通过铰链转动连接壳体5)在壳体5上，以方便收纳，减小设备体积，同时，亦可以实现根据操作人员视觉角度需求调节显示装置7的转动角度，提升用户体验；可理解地，显示装置7的形状和尺寸等可以根据需求进行设定。扫码装置6用于扫描样品4上的的标识码并进行识别，进而将识别得到的数据信息传送给控制器以供使用。扫码装置6的尺寸、形状等亦可以根据需求进行设定。
在一实施例中，如图1和图10所示，所述管路组件31包括用于控制管路组件31中的管路通断的夹管阀311、用于通过实时监控管路中流动的液体中是否混有气泡以判断所述样品4中液体是否流尽的气泡传感器312、用于实时监控管路中液体压力的压力传感器313、用于在运转时带动管路中液体流动的蠕动泵314，以及输出端连接所述蠕动泵314并用于驱动所述蠕动泵314运转的步进电机315；所述夹管阀311、所述气泡传感器312、所述压力传感器313以及所述蠕动泵314均安装在所述液路面板32正面，所述步进电机315安装在所述液路面板32的背面上与所述蠕动泵314相对的位置，且所述步进电机315位于所述壳体5内。也即，将夹管阀311、气泡传感器312、压力传感器313和蠕动泵314安装在液路面板32的正面上，并用螺钉固定；步进电机315安装在液路面板32的背面， 用螺钉固定；蠕动泵314安装在步进电机315上方且位于液路面板32的正面，并用螺钉固定。管路组件31除包括上述元器件之外，还包括连接在各元器件之间的管路。在细胞处理设备处于工作状态下时，步进电机315带动蠕动泵314旋转，作为样品4中的液体流动的动力，驱动液体流动。夹管阀311(可根据需求设置为一个或多个，可以通过电磁驱动开关)起开关作用，管控用于传输样品4中液体的管路中的液体通断及改变流路。气泡传感器312(可根据需求设置为一个或多个)用于实时监控管路中流体是否混有气泡，并将其作为判定样品4中液体是否用完的判定标准。压力传感器313(可根据需求设置为一个或多个)实时监控管路中液体压力大小，若压力大小超出预设压力阈值，控制器在接收到该压力传感器313的压力信号之后，将发出反馈信号，自动控制管路组件31自行调节或者通知该细胞处理设备的对应操作人员进行调整。本申请中的管路组件31开放式布局在液路面板32上，液路面板32可以呈125°倾斜安装在壳体5上，便于操作、安装和拆卸；同时，液路面板32上安装有压力传感器313，监控液路压力，防止出现管路堵塞；液路面板32上还安装有气泡传感器312，监控管路的气泡情况，用于判定样品4中液体是否用完，使得使用过程更为方便。
在一实施例中，如图1和图10所示，细胞处理设备的壳体5上设置的启动按钮51被按下之后，等待几秒钟后开始进入工作状态。此时，可以通过显示装置7的触摸人机交互显示界面进行相关参数设置，使用扫码装置6对样品4上的标识码进行扫码识别，得到数据信息之后发送至控制器，以完成数据信息的录入。之后，通过显示装置7的触摸人机交互显示界面打开液路面板32上的夹管阀311，并将样品4安装好，其中，样品4挂在对应的称重装置1的挂钩123上，将管路与液路面板32上安装的夹管阀311，压力传感器313及气泡传感器312等连接，将离心杯安装在离心模组100的旋转安装座106上，将翻盖26合上以关闭离心腔。在检查样品4安装无误后，在触摸人机交互显示界面上触发启动键以开始对样品4中流入至离心杯的液体进行细胞处理，完成细胞处理后，打开液路面板32上的夹管阀311，将样品4拿出，完成工作。本申请的细胞处理设备体积小，占地面积小，便于安置与搬运；且温度可调范围精确(4℃-40℃)，温度控制精度高(±0.3℃)；同时，该细胞处理设备的功耗低，寿命长，噪音低。
以上仅为本申请的细胞处理设备的实施例而已，并不用以限制本申请，凡在本申请的精神和原则之内所作的任何修改、等同替换和改进等，均应包含在本申请的保护范围之内。

Claims (10)

  1. 一种细胞处理设备，其中，包括：
    称重装置，包括支撑组件和至少一组称重组件，所述支撑组件包括用于支撑所述称重组件的支架；每一组所述称重组件均包括安装在所述支架上的安装支座、安装在所述安装支座上的称重传感器，以及可拆卸安装在所述称重传感器上以悬挂样品的挂钩；
    离心装置，包括具有离心腔的离心锅、设置在所述离心腔内的离心杯、连接所述离心锅并用于带动所述离心杯旋转的离心模组；
    液路模组，包括用于将所述样品内的液体输送到所述离心杯内进行细胞处理的管路组件，以及用于安装和控制所述管路组件中液体流动的液路面板；
    壳体，所述离心锅、所述液路面板和所述支架均安装在所述壳体上。
  2. 根据权利要求1所述的细胞处理设备，其中，所述称重组件为至少两组；所述支架包括间隔设置有安装孔的安装板，所述安装孔的数量与所述称重组件的数量一致；
    所述安装支座将所述称重传感器安装在所述安装板上与所述安装孔相对的位置上，所述挂钩穿过所述安装孔连接所述称重传感器。
  3. 根据权利要求1所述的细胞处理设备，其中，所述挂钩包括折弯段、侧偏段以及连接所述称重传感器的的挂接段，所述折弯段的相对两端分别连接所述挂接段与所述侧偏段；所述折弯段与所述侧偏段围成具有开口的勾持环；
    所述折弯段与所述挂接段的中心轴线均位于第一预设基准平面上，所述侧偏段的中心轴线与所述第一预设基准平面呈预设侧偏角度设置。
  4. 根据权利要求1所述的细胞处理设备，其中，所述离心模组包括旋转轴、轴承组件、联轴器、用于驱动所述旋转轴旋转的驱动电机、用于对所述旋转轴进行制动的制动器以及位于所述离心腔内且用于安装所述离心杯的旋转安装座；所述联轴器连接在所述驱动电机的输出端与所述旋转轴之间；所述旋转轴远离所述联轴器的一端穿过所述轴承组件连接所述旋转安装座；所述制动器连接所述轴承组件。
  5. 根据权利要求4所述的细胞处理设备，其中，所述轴承组件包括具有安装通孔的轴承座以及安装在所述安装通孔的内侧壁上的两个轴承；所述旋转轴穿过两个所述轴承安装在所述轴承座上；所述制动器安装在所述轴承座上；
    所述离心模组还包括安装在所述旋转轴上的不规则轮，所述制动器上设有与所述不规则轮适配的卡槽。
  6. 根据权利要求1所述的细胞处理设备，其中，所述离心装置还包括用于控制所述离心腔温度的温控模组，所述温控模组包括用于对所述离心腔进行制冷的制冷组件，以及用于对所述制冷组件进行散热的散热组件；所述制冷组件的制冷面贴附在所述离心锅的外侧壁上，所述制冷组件的加热面连接所述散热组件。
  7. 根据权利要求6所述的细胞处理设备，其中，所述制冷组件包括制冷面贴附在所述离心锅的第一侧的第一半导体制冷片；所述散热组件包括制冷风扇、连接所述第一半导体制冷片的制热面的散热片，以及连接在所述散热片和所述制冷风扇之间的第一风管；
    所述制冷组件还包括制冷面贴附在所述离心锅的第二侧的第二半导体制冷片；所述第一侧和所述第二侧相对设置；
    所述散热组件还包括散热风扇、第二风管以及连接所述第二半导体制冷片的制热面的热管散热器；所述热管散热器的一端连接所述散热风扇，所述热管散热器与所述散热风扇相对的另一端连接第二风管的进风口。
  8. 根据权利要求1所述的细胞处理设备，其中，所述离心锅的离心腔边缘设有连接翻边；所述离心装置还包括与所述连接翻边贴合连接的密封圈，以及转动连接在所述壳体上 的翻盖，所述翻盖设置在所述离心腔边缘且用于与所述密封圈配合以盖合或打开所述离心腔。
  9. 根据权利要求1所述的细胞处理设备，其中，所述细胞处理设备还包括安装在所述壳体内的控制器、用于对所述样品对应的表示进行扫码识别的扫码装置、用于进行触控操作或显示参数的显示装置，所述称重组件、所述离心装置、所述液路模组、所述扫码装置以及所述显示装置均连接所述控制器。
  10. 根据权利要求1所述的细胞处理设备，其中，所述管路组件包括用于控制管路组件中的管路通断的夹管阀、用于通过实时监控管路中流动的液体中是否混有气泡以判断所述样品中液体是否流尽的气泡传感器、用于实时监控管路中液体压力的压力传感器、用于在运转时带动管路中液体流动的蠕动泵，以及输出端连接所述蠕动泵并用于驱动所述蠕动泵运转的步进电机；
    所述夹管阀、所述气泡传感器、所述压力传感器以及所述蠕动泵均安装在所述液路面板正面，所述步进电机安装在所述液路面板的背面上与所述蠕动泵相对的位置，且所述步进电机位于所述壳体内。
PCT/CN2023/099698 2022-07-22 2023-06-12 细胞处理设备 WO2024016895A1 (zh)

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Publication number Priority date Publication date Assignee Title
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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060226087A1 (en) * 2005-04-08 2006-10-12 Mission Medical, Inc. Method and apparatus for blood separations
WO2015148390A1 (en) * 2014-03-23 2015-10-01 Syngen, Inc. Automated cell washing system and related method
CN207662011U (zh) * 2017-12-04 2018-07-27 重庆中元汇吉生物技术有限公司 一种半导体制冷装置
CN109186052A (zh) * 2018-10-30 2019-01-11 桂林优利特医疗电子有限公司 尿液分析仪恒温控制装置及方法
CN109355176A (zh) * 2018-10-11 2019-02-19 武汉友芝友医疗科技股份有限公司 一种细胞分离设备液路系统
CN210094467U (zh) * 2019-01-28 2020-02-21 嘉兴莱普晟医疗科技有限公司 一种除气泡器官灌注设备
CN211972362U (zh) * 2020-03-06 2020-11-20 湖南开启时代生物科技有限责任公司 一种全自动细胞制备系统
CN112798393A (zh) * 2021-02-01 2021-05-14 中博瑞康（北京）生物技术有限公司 一种大体积生物细胞液浓缩洗涤系统及其方法
CN115418306A (zh) * 2022-07-22 2022-12-02 深圳赛桥生物创新技术有限公司 细胞处理设备

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060226087A1 (en) * 2005-04-08 2006-10-12 Mission Medical, Inc. Method and apparatus for blood separations
WO2015148390A1 (en) * 2014-03-23 2015-10-01 Syngen, Inc. Automated cell washing system and related method
CN207662011U (zh) * 2017-12-04 2018-07-27 重庆中元汇吉生物技术有限公司 一种半导体制冷装置
CN109355176A (zh) * 2018-10-11 2019-02-19 武汉友芝友医疗科技股份有限公司 一种细胞分离设备液路系统
CN109186052A (zh) * 2018-10-30 2019-01-11 桂林优利特医疗电子有限公司 尿液分析仪恒温控制装置及方法
CN210094467U (zh) * 2019-01-28 2020-02-21 嘉兴莱普晟医疗科技有限公司 一种除气泡器官灌注设备
CN211972362U (zh) * 2020-03-06 2020-11-20 湖南开启时代生物科技有限责任公司 一种全自动细胞制备系统
CN112798393A (zh) * 2021-02-01 2021-05-14 中博瑞康（北京）生物技术有限公司 一种大体积生物细胞液浓缩洗涤系统及其方法
CN115418306A (zh) * 2022-07-22 2022-12-02 深圳赛桥生物创新技术有限公司 细胞处理设备

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