WO2024012029A1 - Cartouche de puce intégrée de culture microfluidique par perfusion pour environnement spatial et son procédé de préparation - Google Patents

Cartouche de puce intégrée de culture microfluidique par perfusion pour environnement spatial et son procédé de préparation Download PDF

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Publication number
WO2024012029A1
WO2024012029A1 PCT/CN2023/093003 CN2023093003W WO2024012029A1 WO 2024012029 A1 WO2024012029 A1 WO 2024012029A1 CN 2023093003 W CN2023093003 W CN 2023093003W WO 2024012029 A1 WO2024012029 A1 WO 2024012029A1
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Prior art keywords
perfusion culture
integrated chip
culture
microfluidic perfusion
liquid
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PCT/CN2023/093003
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English (en)
Chinese (zh)
Inventor
邓玉林
徐建栋
张莉明
任培
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北京理工亘舒科技有限公司
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Publication of WO2024012029A1 publication Critical patent/WO2024012029A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/54—Constructional details, e.g. recesses, hinges hand portable
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes

Definitions

  • the present disclosure belongs to the field of biomedical detection, and specifically relates to a microfluidic perfusion culture integrated chip cartridge for space environment and a preparation method thereof.
  • the perfusion culture chip and perfusion equipment in the related art require liquid pipeline connection, and it is inconvenient to replace the chip and reconnect the pipeline in a space environment. If the chip is connected to an external perfusion pipeline and is exposed inside the device, there is a risk of liquid leakage, which will affect the normal operation of the device and even pollute the space environment. If the chip and perfusion equipment are integrated, the mechanical and control electrical components cannot be reused, resulting in a waste of load.
  • Some embodiments of the present disclosure provide a replaceable microfluidic perfusion culture integrated chip cartridge suitable for space environments.
  • the microfluidic perfusion culture integrated chip cartridge provided by some embodiments of the present disclosure integrates the culture medium, culture chamber, liquid flow path, control valve and other components required for microbial culture into the microfluidic chip cartridge, providing a comprehensive
  • the closed microbial culture and detection environment reduces the difficulty of manual replacement of microbial culture chips and at the same time avoids the risk of biological sample leakage during the replacement process.
  • Some embodiments of the present disclosure provide an integrated chip cartridge for microfluidic perfusion culture in space environments, which includes a cartridge frame structure, a microfluidic perfusion culture module and an airtight electrical connector.
  • the cartridge frame structure is composed of a middle frame plate , the upper and lower covers form a fully sealed structure, the microfluidic perfusion culture module is enclosed inside the cartridge frame structure, and the cartridge frame structure is connected to external control equipment through an airtight electrical connector.
  • the microfluidic perfusion culture module and the air-tight electrical connector are provided in the middle frame plate, and annular sealing grooves are provided on both sides of the middle frame plate corresponding to the upper and lower cover plates. There is a sealing ring, and the upper and lower cover plates and the middle frame plate are fixed with bolts.
  • the microfluidic perfusion culture module includes a liquid storage unit, a syringe pump unit, a liquid dispensing control unit and a culture detection chamber unit.
  • the culture detection chamber unit is fixed on the liquid dispensing control unit.
  • the culture detection chamber unit consists of an upper cover plate and a middle well plate.
  • the upper cover plate and the middle well plate form a sealed culture chamber.
  • the culture chamber and the flow path are connected through the filter membrane.
  • the liquid storage bag and waste liquid bag of the liquid storage unit are fixed in the middle frame plate through bolts, and the liquid storage port and waste liquid port are respectively connected to the liquid inlet channel of the liquid dispensing control unit.
  • the syringe pump unit On the liquid outlet channel, the syringe pump unit is connected to the dispensing control unit through a Luer connector and is connected to the liquid path of the liquid storage bag.
  • the syringe pump body passes through the side wall of the middle frame plate, and the inside of the middle frame plate corresponds to the syringe pump body. There is a sealing groove, and an O-ring is provided in the sealing groove.
  • hollow sealing screws are provided on the outside of the middle frame plate to fix the syringe pump body extending out of the middle frame plate, and removable limit blocks are provided on the outside of the middle frame plate for limiting storage. The plunger of the syringe pump moves laterally.
  • the liquid separation control unit is provided with a solenoid valve to control the opening and closing of the liquid inlet channel and the liquid outlet channel.
  • the length of the cartridge frame structure is 150-190mm, the width is 80-100mm, and the thickness is 20-25mm.
  • the volume of the culture detection chamber is 200-300uL, and the liquid storage bag, waste The volume of the liquid bag is 5-10mL, and the volume of the syringe pump is 200-2000uL.
  • the length of the cartridge frame structure may be 155 mm
  • the width of the cartridge frame structure may be 85 mm
  • the thickness of the cartridge frame structure may be 24 mm.
  • the volume of the culture detection chamber may be 300 uL.
  • the volume of the liquid storage bag of the liquid storage unit may be 6 mL, and the volume of the waste liquid bag of the liquid storage unit may be 6 mL.
  • the volume of the syringe pump of the syringe pump unit may be 1 mL.
  • a handle is provided on the side of the cartridge frame structure.
  • the air-tight electrical connector is fixed on the middle frame plate, the air-tight electrical connector is connected to the heating plate, the temperature sensor and the solenoid valve respectively, and is plugged into the control device externally.
  • the airtight electrical connector can be connected to an external controller to control the electrical components inside the microfluidic perfusion culture integrated chip cartridge.
  • the airtight electrical connector can be connected to an external power source.
  • Some embodiments of the present disclosure also provide a method for preparing an integrated chip cartridge for microfluidic perfusion culture in a space environment, including the following steps:
  • Step 1 Assemble the microfluidic perfusion culture module and connect the liquid storage unit, syringe pump unit, and culture detection chamber unit
  • each functional unit On the liquid separation control unit, each functional unit is pre-installed with microbial dry powder, culture medium, etc. as required, and the leads of the solenoid valve, heating film and temperature sensor are connected to an airtight electrical connector;
  • Step 2 Fixedly connect the microfluidic perfusion culture module to the middle frame plate, including the fixation of the liquid storage bag, waste liquid bag and their pipelines;
  • Step 3 The syringe pump passes through the middle frame plate, and the O-ring is placed outside the syringe pump body.
  • the syringe pump is locked and fixed on the Luer connector.
  • the O-ring is fed into the sealing groove of the middle frame plate.
  • the hollow sealing screw set is fixed on the syringe pump. Outside the body, the limit block snaps into the push rod;
  • Step 4 Install sealing rings in the annular sealing grooves on both sides of the middle frame plate, and connect the upper and lower cover plates to the middle frame plate with bolts.
  • microfluidic perfusion culture integrated chip cartridge is fully enclosed, and the microorganisms inside are isolated from outside operators, making it suitable for use in space environments.
  • the culture medium, perfusion pump, growth detection chamber, constant temperature components and waste liquid storage required for microbial growth are all designed as unit components and integrated in the module.
  • the module separately builds the sterilized culture medium and freeze-dried bacterial powder and stores them in a cold storage. Easy to operate, adopts modular design and works with external control equipment. When using it, the operator only needs to take the card box out of the package, insert the external device as required, and start the program.
  • Figure 1 is a schematic structural diagram of a microfluidic perfusion culture integrated chip cartridge provided by some embodiments of the present disclosure
  • Figure 2 is a schematic structural diagram of a microfluidic perfusion culture module provided by some embodiments of the present disclosure
  • Figure 3 is a schematic side structural view of a culture detection chamber provided by some embodiments of the present disclosure.
  • 1-cassette frame structure 2-airtight electrical connector, 3-microfluidic perfusion culture module, 4-culture detection chamber unit, 41-upper cover, 42-heating plate, 43-filtration Membrane, 44-sealing ring, 5-liquid storage bag, 6-waste liquid bag, 7-syringe pump body, 8-hollow sealing screw, 9-limit block, 10-solenoid valve, 11-handle.
  • the microfluidic perfusion culture integrated chip cartridge includes a cartridge frame structure 1, a microfluidic perfusion culture module 3 and an airtight electrical connector 2.
  • the cartridge frame structure can be formed into a fully sealed structure by the middle frame plate, the upper cover plate and the lower cover plate, and the microfluidic perfusion culture module 3 can be enclosed inside the cartridge frame structure 1 .
  • the cartridge frame structure 1 can Connect to external control equipment through airtight electrical connector 2.
  • the microfluidic perfusion culture module 3 and the airtight electrical connector 2 can be arranged in the middle frame plate, and the middle frame plate can be provided with annular sealing grooves on both sides corresponding to the upper and lower cover plates.
  • a sealing ring can be provided in the annular sealing groove, and the upper and lower cover plates and the middle frame plate can be fixed by bolts.
  • the microfluidic perfusion culture module 3 can be used to culture and detect biological samples, and the airtight electrical connector 2 can be used to connect to an external power supply and controller to control the electrical components inside the integrated chip card box.
  • the perfusion culture cartridge is controlled to achieve a constant culture temperature, resuscitate the microorganisms to be cultured, provide regular and quantitative culture medium perfusion, and detect the OD value and fluorescence value data of the microorganisms.
  • the length of the cartridge frame structure may be 150-190 mm, the width may be 80-100 mm, and the thickness may be 20-25 mm. In other optional embodiments, the length of the cartridge frame structure may be 155 mm, the width may be 85 mm, and the thickness may be 24 mm.
  • a handle can be provided on the side of the card box frame structure. The operator only touches the outer surface of the perfusion culture cartridge to avoid microbial culture failure due to contamination introduced by personnel during operation; at the same time, the microorganisms inside the cartridge will not leak into the environment where the operator is located to avoid contamination of the environment.
  • the microfluidic perfusion culture module may include a liquid storage unit, a syringe pump unit, a liquid dispensing control unit and a culture detection chamber unit.
  • the culture detection chamber unit 4 can be fixed on the liquid dispensing control unit.
  • the culture detection chamber unit may be composed of an upper cover plate 41 and a middle well plate to form a sealed culture chamber.
  • a heating plate 42 and a temperature sensor may be provided between the upper cover plate and the middle orifice plate.
  • a microbial filter membrane 43 and a sealing gasket 44 are provided between the middle orifice plate and the liquid separation control unit located below the middle orifice plate, and the culture chamber is connected to the flow path through the microbial filter membrane.
  • the volume of the culture detection chamber can be 200 ⁇ 300uL. In some optional embodiments, the volume of the culture detection chamber is 300uL.
  • the liquid storage bag 5 and waste liquid bag 6 of the liquid storage unit can be fixed in the middle frame plate through bolts.
  • the liquid storage port of the liquid storage bag 5 and the waste liquid port of the waste liquid bag 6 are respectively connected to the liquid inlet channel and the liquid outlet channel of the liquid dispensing control unit.
  • the volumes of the liquid storage bag and waste liquid bag can be 5 to 10 mL respectively. In some optional embodiments, the volume of the liquid storage bag is 6 mL. In some optional embodiments, the volume of the waste liquid bag is 6 mL.
  • the syringe pump body 7 can be placed in the middle frame plate, fixed on the liquid dispensing control unit through a Luer connector, and connected to the liquid path of the liquid storage bag.
  • the volume of the syringe pump can be 1-2mL. In some optional embodiments, the volume of the syringe pump is 1 mL.
  • the syringe pump body can pass through the side wall of the middle frame plate.
  • the inner side of the middle frame plate is provided with a sealing groove at a position corresponding to the syringe pump body, and an O-ring is provided in the sealing groove.
  • Hollow sealing screws 8 can be provided on the outside of the middle frame plate to fix the syringe pump body extending out of the middle frame plate.
  • a removable limiting block 9 can be provided on the outside of the middle frame plate to limit the lateral movement of the push rod of the syringe pump during storage.
  • the liquid separation control unit may be provided with a solenoid valve to control the opening and closing of the liquid inlet channel and the liquid outlet channel.
  • the number of solenoid valves can be adapted to the number of culture chambers.
  • the air-tight electrical connector can be fixed on the middle frame plate.
  • the air-tight electrical connector Heating plates, temperature sensors and/or solenoid valves can be connected separately and plugged into external control devices.
  • the control device can provide switch control of the two-way solenoid valve, real-time temperature reading of the temperature sensor and/or power supply of the heating film, etc.
  • the external control device also provides the push and pull power of the syringe pump.
  • the temperature sensor monitors the temperature of the culture detection chamber unit, and feedback adjusts the heating film so that the temperature of the chamber reaches and remains constant at 37°C;
  • the device pulls the syringe pump push rod of the chip cartridge and absorbs 800uL of culture medium in the liquid storage bag;
  • valve 1 and valve 2 Close valve 1 and valve 2 and keep it at a constant temperature of 37°C for 1 hour to allow the dry microbial powder to completely recover.
  • the device pulls the syringe pump push rod of the chip cartridge and absorbs 800uL of culture medium in the liquid storage bag;
  • valve 1 and valve 2 Close valve 1 and valve 2, maintain static culture for 10 minutes, and then repeat steps c and d to complete intermittent continuous perfusion;
  • test can be completed before each perfusion, or continuous testing can be performed;
  • the entire perfusion culture process can last 8 to 12 hours according to experimental needs. If the amount of liquid in the syringe pump is insufficient during the process, you can repeat step b and continue to draw from the liquid storage bag.
  • Some embodiments of the present disclosure also provide a method for preparing an integrated chip cartridge for microfluidic perfusion culture in a space environment, including the following steps:
  • Step 1 Assemble the microfluidic perfusion culture module.
  • the assembly process includes connecting the liquid storage unit, syringe pump unit, and culture detection chamber unit to the liquid separation control unit.
  • Each functional unit is pre-installed with microbial dry powder, culture medium, etc. as needed.
  • the leads of the solenoid valve, heating film and temperature sensor are connected to an airtight electrical connector;
  • Step 2 Fixedly connect the microfluidic perfusion culture module to the middle frame plate, including the fixation of the liquid storage bag, waste liquid bag and their pipelines;
  • Step 3 The syringe pump passes through the middle frame plate, and the O-ring is placed outside the syringe pump body.
  • the syringe pump is locked and fixed on the Luer connector.
  • the O-ring is fed into the sealing groove of the middle frame plate.
  • the hollow sealing screw set is fixed on the syringe pump. Outside the body, the limit block snaps into the push rod;
  • Step 4 Install sealing rings in the annular sealing grooves on both sides of the middle frame plate, and connect the upper and lower cover plates to the middle frame plate with bolts.
  • the cartridge provided by some embodiments of the present disclosure can provide overall sealing, and even when the internal pressure of the cartridge reaches 1.2 atmospheres, it can still ensure that the contents of the cartridge will not leak.
  • the cartridge according to some embodiments of the present disclosure can ensure that there is no risk of leakage in a vacuum environment or in extreme situations when the external pressure of the cartridge is completely lost.
  • Some embodiments of the present disclosure provide a microfluidic perfusion culture integrated chip cartridge for space environment and a preparation method thereof.
  • the cartridge includes a cartridge frame structure, a microfluidic perfusion culture module and an airtight electrical connector.
  • the cartridge frame structure is composed of a middle frame plate, an upper cover plate and a lower cover plate to form a fully sealed structure.
  • the microfluidic perfusion culture module The module is enclosed within the cartridge frame structure, and the cartridge frame structure is connected to external control equipment through an airtight electrical connector.
  • the microfluidic perfusion culture integrated chip cartridge provided by some embodiments of the present disclosure is fully enclosed, and the microorganisms inside are isolated from external operators, and is suitable for use in space environments.
  • the microfluidic perfusion culture integrated chip cartridge provided by some embodiments of the present disclosure is fully integrated.
  • the culture medium, perfusion pump, growth detection chamber, constant temperature components and waste liquid storage required for microbial growth are all designed as unit components and integrated in in the module.
  • the microfluidic perfusion culture integrated chip cartridge provided by some embodiments of the present disclosure is easy to store.
  • the module separately builds the sterilized culture medium and freeze-dried bacterial powder and stores them in cold storage.
  • the microfluidic perfusion culture integrated chip cartridge provided by some embodiments of the present disclosure is easy to operate, adopts a modular design, and works with external control equipment. When using it, the operator only needs to take the card box out of the package, insert the external device as required, and start the program.
  • microfluidic perfusion culture integrated chip cartridge for space environment of the present application is reproducible and can be used in a variety of industrial applications.
  • the microfluidic perfusion culture integrated chip cartridge of the present application for space environment can be used in any device that requires bacterial culture.

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Abstract

Cartouche de puce intégrée de culture par perfusion microfluidique pour environnement spatial, comprenant un corps structurel de cadre de cartouche, un module de culture microfluidique par perfusion et un connecteur électrique étanche à l'air. Le corps structurel du cadre de la cartouche est composé d'une structure à étanchéité totale constituée d'une plaque de cadre intermédiaire, d'une plaque de couverture supérieure et d'une plaque de couverture inférieure, et le module de culture microfluidique par perfusion est scellé dans le corps structurel du cadre de la cartouche. Le corps structurel du cadre de la cartouche est relié à un dispositif de commande externe au moyen d'un connecteur électrique étanche à l'air. La cartouche de puce intégrée de culture par perfusion microfluidique est entièrement étanche et les micro-organismes qu'elle contient sont isolés des opérateurs extérieurs, de sorte que la cartouche peut être utilisée dans un environnement spatial.
PCT/CN2023/093003 2022-07-11 2023-05-09 Cartouche de puce intégrée de culture microfluidique par perfusion pour environnement spatial et son procédé de préparation WO2024012029A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202210808260.6 2022-07-11
CN202210808260.6A CN115011473A (zh) 2022-07-11 2022-07-11 一种空间环境用微流控灌流培养集成芯片卡盒及其制备方法

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CN115011473A (zh) * 2022-07-11 2022-09-06 北京理工亘舒科技有限公司 一种空间环境用微流控灌流培养集成芯片卡盒及其制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020055166A1 (en) * 2000-10-02 2002-05-09 Cannon Thomas F. Automated bioculture and bioculture experiments system
CN112457985A (zh) * 2020-12-16 2021-03-09 北京理工亘舒科技有限公司 一种灌流培养芯片及灌流系统
CN112481077A (zh) * 2020-12-01 2021-03-12 北京理工大学 一种微流控灌流培养装置及其灌流方法
CN113388583A (zh) * 2021-06-25 2021-09-14 北京理工大学 一种用于空间环境的片上原位细胞复苏、悬浮培养的方法及其微流控芯片和装置
CN115011473A (zh) * 2022-07-11 2022-09-06 北京理工亘舒科技有限公司 一种空间环境用微流控灌流培养集成芯片卡盒及其制备方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020055166A1 (en) * 2000-10-02 2002-05-09 Cannon Thomas F. Automated bioculture and bioculture experiments system
CN112481077A (zh) * 2020-12-01 2021-03-12 北京理工大学 一种微流控灌流培养装置及其灌流方法
CN112457985A (zh) * 2020-12-16 2021-03-09 北京理工亘舒科技有限公司 一种灌流培养芯片及灌流系统
CN113388583A (zh) * 2021-06-25 2021-09-14 北京理工大学 一种用于空间环境的片上原位细胞复苏、悬浮培养的方法及其微流控芯片和装置
CN115011473A (zh) * 2022-07-11 2022-09-06 北京理工亘舒科技有限公司 一种空间环境用微流控灌流培养集成芯片卡盒及其制备方法

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