WO2023246199A1 - 全自动pcr荧光定量分析仪 - Google Patents

全自动pcr荧光定量分析仪 Download PDF

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Publication number
WO2023246199A1
WO2023246199A1 PCT/CN2023/082323 CN2023082323W WO2023246199A1 WO 2023246199 A1 WO2023246199 A1 WO 2023246199A1 CN 2023082323 W CN2023082323 W CN 2023082323W WO 2023246199 A1 WO2023246199 A1 WO 2023246199A1
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WO
WIPO (PCT)
Prior art keywords
cover
hot
track
temperature
plate
Prior art date
Application number
PCT/CN2023/082323
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English (en)
French (fr)
Inventor
胡志丹
张健
王亮
陈瀚
Original Assignee
中元汇吉生物技术股份有限公司
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Application filed by 中元汇吉生物技术股份有限公司 filed Critical 中元汇吉生物技术股份有限公司
Publication of WO2023246199A1 publication Critical patent/WO2023246199A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/10—Apparatus for enzymology or microbiology rotatably mounted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control

Definitions

  • the invention belongs to the technical field of medical devices, and is specifically a fully automatic PCR fluorescence quantitative analyzer.
  • PCR polymerase chain reaction
  • the speed of DNA/RNA cycle amplification time determines whether the entire equipment is market competitive.
  • the amplification process requires the unwinding and replication of DNA/RNA at different temperatures.
  • the temperature control modules of existing PCR fluorescence quantitative analyzers are all set to a single fixed structure, that is, the different temperatures required for the amplification process are all realized on one module by raising and lowering the temperature. In this way, the amplification time of the entire cycle cannot be shortened. It cannot meet the needs of scientific research and clinical diagnosis for rapid amplification cycle detection.
  • the existing PCR fluorescence quantitative analyzer uses a rotary motor and a linear screw motor to drive the PCR hot cover to rotate and lock. Although it can meet the technical purpose of automatic flipping and locking of the PCR hot cover to a certain extent, it uses two The motor has complex structure and is not conducive to later maintenance. In addition, the existing flip-type hot cover also has the problem of requiring a large space for operation, which is not conducive to the internal space layout design of the analyzer.
  • the purpose of the present invention is to provide a fully automatic PCR fluorescence quantitative analyzer that can achieve rapid switching of the required temperature by setting multiple temperature zones to meet the needs of rapid amplification cycle detection.
  • a fully automatic PCR fluorescence quantitative analyzer including a rack.
  • the rack is provided with a temperature control system and a photoelectric detection module.
  • the temperature control system includes a hot cover module and a temperature control device located below the hot cover module. module;
  • the temperature control module includes a support plate, and at least two temperature control modules are arranged side by side on the support plate; the temperature control module includes a temperature control base, and a hot well seat is provided on the temperature control base. A hot well is provided on the base; a temperature control component for controlling the temperature in the hot well is provided between the temperature adjustment base and the hot well base;
  • the hot cover module includes a hot cover assembly and a bracket assembly.
  • the bracket assembly includes a bracket main body.
  • a sample tube placement plate is installed on the bracket body.
  • the hot cover assembly is arranged above the sample tube placement plate and connected with the sample tube placement plate.
  • the sample tube is placed between the plates to realize opening and closing;
  • the temperature control system also includes a temperature zone switching driving mechanism for driving the relative movement between the bracket body and the support plate in the horizontal direction so that the sample tube placing plate is located directly above the different temperature adjustment modules. ;
  • the temperature control system also includes a device for driving the relative movement between the bracket body and the support plate in the vertical direction so that the sample tube placed in the sample tube placement plate falls downward into the hot well or a switching drive mechanism that is moved upward from the hot well;
  • the hot well is provided with two optical fiber connection ports, and the photoelectric detection module is provided with an optical fiber fixed disk.
  • the optical fiber fixed disk is provided with an optical fiber receiving interface and an optical fiber excitation interface.
  • the optical fiber receiving interface and the optical fiber excitation interface are connected with two Optical fibers are respectively provided between the optical fiber connection ports.
  • the temperature control assembly includes a temperature control element for controlling temperature rise and fall, and a first thermal conductive layer is provided between the temperature control element and the hot well seat.
  • a heat sink is provided on the temperature adjustment base, and a second thermal conductive layer is provided between the temperature adjustment base and the temperature control element.
  • a cooling fan is provided below the support plate, and cooling air ducts are provided on both sides of the heat sink.
  • the temperature adjustment base is provided with a hot well positioning block for positioning the hot well seat.
  • the hot well seat is provided with an optical fiber positioning seat for positioning the optical fiber and an optical fiber pressing plate for compressing and fixing the optical fiber on the optical fiber positioning seat.
  • the temperature adjustment base is provided with a mounting hole for installing a temperature sensor
  • the hot well seat is provided with a hot well temperature sensor for measuring the temperature of the hot well
  • the temperature control assembly The power supply circuit is equipped with a first over-temperature protection switch.
  • the temperature zone switching driving mechanism includes a linear track and a linear driving mechanism for driving the bracket body to move along the linear track.
  • the main body of the bracket is provided with opening and closing guide rods respectively located on both sides of the sample tube placement plate, and the heat cover assembly is in sliding cooperation with the opening and closing guide rods;
  • a hot cover track is provided above the linear track for guiding the hot cover assembly.
  • the hot cover track includes a first section of the hot cover track and a second section of the hot cover track that are parallel to the linear track.
  • the first section of the hot cover track is The distance between the linear track and the linear track is greater than the distance between the second section of the heated cover track and the linear track, and a connecting section is provided between the first section of the heated cover track and the second section of the heated cover track;
  • the heat cover assembly When the heat cover assembly is located on the first section of the heat cover track, the heat cover assembly is located above the sample tube placement plate; when the heat cover assembly is located on the second section of the heat cover track, the heat cover assembly is located on the second section of the heat cover track.
  • the hot cover assembly covers the sample tube placing plate.
  • a bracket position detection component for detecting the moving position of the bracket body on the linear rail is provided between the bracket body and the linear track.
  • the linear drive mechanism includes a first pulley and a second pulley respectively located at both ends of the linear track, a synchronous belt is set between the first pulley and the second pulley, and the synchronous belt is connected to the first pulley and the second pulley.
  • the bracket body is fixedly connected, and the linear drive mechanism further includes a drive motor for driving the first pulley or the second pulley to rotate.
  • guide plates are provided on both sides of the bracket body, and the linear rails and the hot cover rails are respectively provided on the guide plates; or, guide plates are provided on both sides of the bracket body, and the guide plates are respectively provided on both sides of the bracket body.
  • a bottom plate is provided below the plate, the linear track is arranged on the bottom plate, and the heat cover track is arranged on the guide plate.
  • the hot cover track is a guide groove opened on the guide plate, and the hot cover assembly is provided with a guide shaft that cooperates with the guide groove.
  • the guide shaft is equipped with a bearing that is in rolling fit with the guide groove.
  • the hot cover assembly is provided with a linear bearing that cooperates with the opening and closing guide rod.
  • opening and closing guide rod and the linear track are perpendicular to each other.
  • the angle between the connecting section and the hot cover track section is 110-150°.
  • the main body of the bracket is provided with a rail for entering and exiting the warehouse and sliding with the rail for entering and exiting the warehouse.
  • the sample tube placing plate is installed on the movable bracket with a matching movable bracket, and the main body of the bracket is provided with a device for driving the movable bracket to move along the entry and exit bin track to drive the sample tube placing plate from the movable bracket.
  • the lower part of the hot cover assembly extends or retracts to the entry and exit driving mechanism of the lower part of the hot cover assembly.
  • the drive mechanism for entering and exiting the warehouse includes a threaded screw rod parallel to the rail for entering and exiting the warehouse.
  • the movable bracket is provided with a nut seat that threadably matches the threaded screw rod.
  • the main body of the bracket is equipped with a screw for driving all the screws.
  • the screw motor rotates the threaded screw.
  • an entry and exit position detection component for detecting the moving position of the movable bracket is provided between the movable bracket and the bracket body.
  • the movable bracket is equipped with a sample bracket that moves synchronously with it, the sample tube placement plate is installed on the sample bracket, and the sample bracket is provided with a bracket heating film located below the sample tube placement plate,
  • the sample holder is made of heat-insulating material.
  • the opening and closing driving mechanism includes a height adjustment plate located below the support plate, and the support plate moves synchronously with the height adjustment plate; a screw nut is provided on the height adjustment plate, and the screw nut There is a threaded screw rod inside that matches its thread;
  • It also includes a height adjustment driving mechanism that is drivingly connected to the threaded screw rod to drive the threaded screw rod to rotate.
  • the heat cover assembly includes a heat cover and a heat cover upper frame located above the heat cover.
  • a heat cover guide rod is provided between the heat cover and the heat cover upper frame.
  • One end of the heat cover guide rod is connected to the heat cover upper frame.
  • the hot cover is fixedly connected, and the other end is in sliding fit with the upper frame of the hot cover.
  • the guide rod of the hot cover is equipped with a spring located between the hot cover and the upper frame of the hot cover.
  • the heat cover includes a heat cover cover plate and a heat cover heat insulation plate located above the heat cover cover plate, and a heat cover heating film is provided between the heat cover cover plate and the heat cover heat insulation plate.
  • the heat cover further includes a temperature sensor for measuring the temperature of the cover plate of the heat cover and a second over-temperature protection switch provided on the heating circuit of the heating film of the heat cover.
  • the power supply module is located above the electric control module, and the electric control module is located above the photoelectric detection module.
  • it also includes a display screen located above the hot cover module.
  • the fully automatic PCR fluorescence quantitative analyzer of the present invention installs a temperature control system on the rack.
  • the system and photoelectric detection component can be used to directly detect samples that have been cyclically amplified by the temperature control system; among them, the temperature control system includes a hot cover module and a temperature control module. By setting at least two adjusters in the temperature module, Temperature module, so that during use, the temperature control component can be used to adjust the temperature in the corresponding hot well to the set temperature.
  • the temperature zone switching drive mechanism is used to drive the main body of the bracket Move relative to the support plate in the horizontal direction, so that the sample tube placing plate can be located directly above different temperature adjustment modules, that is, the sample tube placing plate can be located in different temperature zones, realizing rapid switching of temperature zones; using the opening and closing
  • the driving mechanism drives the bracket body and the support plate to move relative to each other in the vertical direction, so that the sample tube placed on the sample tube placement plate can be driven to drop downward into the corresponding heat zone after the sample tube is switched to the corresponding temperature zone.
  • the sample tube is driven to move out from above the corresponding hot well to separate the sample tube and the hot well to facilitate temperature zone switching.
  • the sample can be driven in Different temperature zones to achieve thermal cycle; under the action of the opening and closing guide rod, the heat cover assembly also moves in the direction parallel to the linear track. At the same time, under the guidance of the heat cover track, the heat cover is placed on a section of the heat cover track.
  • the upper shelf of the hot cover can move downward or downward along the opening and closing guide rod relative to the main body of the bracket between the first section of the hot cover track and the second section of the hot cover track. Move upward to achieve the technical purpose of covering the hot cover on the sample tube placement plate or opening the hot cover from the sample tube placement plate.
  • the entry and exit drive mechanism can be used to drive the movable bracket to move along the entry and exit rails. Since the sample tube placement plate is installed on the movable bracket on the top, and then drive the sample tube placement plate to move along the track in and out of the warehouse. In this way, the sample tube placement plate can be exposed from below the hot cover to facilitate the placement of the sample tube. After the sample tube placement is completed, the sample tube can be placed again. The plate is returned under the heated lid.
  • the technical purpose of heating the sample placed on the sample tube placement plate can be achieved, which is the same as the heating cover heating film.
  • the heating effect is better; the sample holder is made of heat-insulating material, which can prevent heat diffusion from affecting other components.
  • Figure 1 is a schematic structural diagram of an embodiment of a fully automatic PCR fluorescence quantitative analyzer of the present invention
  • Figure 2 is a three-dimensional view of the fully automatic PCR fluorescence quantitative analyzer of this embodiment
  • Figure 3 is a schematic structural diagram of the temperature control system
  • Figure 4 is a schematic structural diagram of the temperature control module
  • Figure 5 is a perspective view of the temperature control module
  • Figure 6 is a schematic structural diagram of the temperature control module
  • Figure 7 is a perspective view of the temperature control module
  • Figure 8 is a right side view of the thermal cover module embodiment
  • Figure 9 is a left side view of the hot cover module
  • Figure 10 is a schematic structural diagram of the guide plate
  • Figure 11 is a schematic structural diagram of the bracket assembly
  • Figure 12 is an axial view of the bracket assembly
  • Figure 13 is a schematic structural diagram of the thermal cover assembly
  • Figure 14 is a schematic structural diagram of the photoelectric detection module.
  • 20-Bracket body 21-Sample tube placement plate; 22-Opening and closing guide rod; 23-In and out of the warehouse track; 24-Mobile bracket; 25-Threaded screw; 26-Nut seat; 27-Screw motor; 28-No. Two photocoupler sensors; 29-second light barrier; 30-sample holder; 31-top plate;
  • 40-hot cover module 41-linear track; 42-hot cover track; 42a-hot cover track first section; 42b-hot cover track second section; 42c-connection section; 43-first photocoupler sensor; 44-first gear Light sheet; 45-first pulley; 46-second pulley; 47-synchronous belt; 48-driving motor; 49-guide plate; 49a-base plate.
  • 50-temperature control module ; 51-support plate; 51a-low temperature zone; 51b-Chinese zone; 51c-high temperature zone; 52-temperature adjustment base; 53-hot well seat; 54-hot well; 55-temperature control element; 56 -First thermal conductive layer; 57-heat sink; 58-second thermal conductive layer; 59-cooling fan; 60-cooling air duct; 61-heat well positioning block; 62-fiber; 63-fiber connection port; 64-fiber positioning Seat; 65-fiber pressure plate; 66-mounting hole; 67-hot well temperature sensor; 68-over-temperature protection switch; 69-height adjustment plate; 70-screw nut; 71-threaded screw; 72-height adjustment motor; 73-Transmission belt; 74-Temperature adjustment module; 75-Hot well seat pressure plate.
  • 80-Photoelectric detection module 81-Fixed optical fiber disk; 82-Fluorescence component rotating disk; 83-Receiving lens barrel assembly; 84-Excitation lens barrel assembly; 85-Conductive slip ring; 86-Motor; 87-Third optical coupler sensor ;
  • the fully automatic PCR fluorescence quantitative analyzer of this embodiment includes a rack 100.
  • the rack 100 is provided with a temperature control system, a photoelectric detection module 80, a power supply module 101, an electrical control module 102 and Display 103.
  • the power module 101 is located above the electronic control module 102
  • the electronic control module 102 is located above the photoelectric detection module 80
  • the display screen 103 is above the temperature control system.
  • the temperature control system of this embodiment includes a heat cover module 40 and a temperature control module 50 arranged below the heat cover module 40 .
  • the display screen 103 is located above the heat cover module 40 .
  • the temperature control module of this embodiment includes a support plate 51, and at least two temperature adjustment modules 74 are arranged side by side on the support plate 51. There are three temperature regulating modules 74 in this embodiment. During use, the temperatures of the three temperature regulating modules are controlled respectively so that the three temperature regulating modules form a low temperature zone 51a, a medium temperature zone 51b and a high temperature zone 51c in sequence. Specifically, the number of temperature adjustment modules 74 can be set according to actual needs, such as setting the number of temperature adjustment modules 74 to 2, 4, or more, which will not be described again. Specifically, as shown in Figures 6 and 7, the temperature control module of this embodiment includes a temperature control base 52.
  • the temperature control base 52 is provided with a hot well seat 53, and the hot well seat 53 is provided with a hot well 54.
  • a temperature control component for controlling the temperature in the hot well 54 is provided between the temperature adjustment base 52 and the hot well seat 53.
  • the temperature control component can be used to adjust the temperature of the hot well seat 53 and thereby the temperature of the hot well 54.
  • the temperature control component mainly plays the role of adjusting the temperature, which can be implemented in various existing ways.
  • the temperature control component includes a temperature control element 55 for controlling the temperature rise and fall.
  • the temperature control element 55 and the hot well seat 53 There is a first thermal conductive layer 56 between them.
  • the temperature control element 55 adjusts the temperature and uses the first thermal conductive layer 56 to quickly transfer heat to the hot well seat 53 to achieve rapid temperature adjustment of the hot well seat 53 .
  • the temperature regulating base 52 of this embodiment is provided with a heat sink 57, and a second thermal conductive layer 58 is provided between the temperature regulating base 52 and the temperature control element 55. When heat dissipation is required, the heat can also pass through the second thermal conductive layer. 58 is quickly transferred to the heat sink 57 to achieve heat dissipation and cooling.
  • the temperature control element 55 of this embodiment uses a semiconductor refrigerator, and the first thermal conductive layer 56 and the second thermal conductive layer 58 are both made of graphene material.
  • a cooling fan 59 is provided below the support plate 51 , and cooling air ducts 60 are provided on both sides of the heat sink 57 .
  • the cooling air ducts 60 located on both sides of the three temperature control modules are integrated.
  • the temperature adjustment base 52 of this embodiment is provided with a hot well positioning block 61 for positioning the hot well seat 53, so as to realize the positioning and installation of the hot well seat 53 so as to facilitate the connection with the sample tubes placed in the sample tube placement plate 21.
  • the hot well seat 53 of this embodiment is connected to each other.
  • the hot well seat 53 of this embodiment is fixedly installed on the temperature regulating base 52 through the hot well seat pressure plate 75 .
  • the temperature control base 52 of this embodiment is provided with a mounting hole 66 for installing a temperature sensor.
  • the hot well seat 53 is provided with a hot well temperature sensor 67 for measuring the temperature of the hot well 54.
  • the temperature control assembly A first over-temperature protection switch 68 is provided on the power supply circuit.
  • the hot well 54 of this embodiment is provided with an optical fiber connection port 63 for connecting the optical fiber 62
  • the hot well base 53 is provided with an optical fiber positioning seat 64 for positioning the optical fiber 62 and the optical fiber 62. Press the optical fiber pressing plate 65 fixed on the optical fiber positioning base 64 to improve the connection reliability with the optical fiber 62 .
  • the optical fiber connection port 63 can be provided only on the hot wells 73 of the temperature control modules located in the high temperature zone 51c and the medium temperature zone 51b. In other embodiments, the optical fiber connection port 63 can also be provided on the heat wells 73 of all the temperature control modules.
  • the optical fiber connection port 63 is provided on the well 73. In this embodiment, the optical fiber connection port 63 is only provided on the hot well 73 of the temperature control module located in the high temperature zone 51c and the medium temperature zone 51b.
  • the hot cover module of this embodiment includes a hot cover assembly and a bracket assembly.
  • the bracket assembly includes a bracket body 20.
  • a sample tube placing plate 21 is installed on the bracket body 20.
  • the hot cover assembly is arranged on the sample tube placing plate 21. Above and between the sample tube placement plate 21 to achieve opening and closing.
  • the temperature control system of the PCR fluorescence quantitative analyzer of this embodiment also includes a device for driving the relative movement between the bracket body 20 and the support plate 51 in the horizontal direction so that the sample tube placement plate 21 is located directly above the different temperature adjustment modules.
  • Temperature zone switching drive mechanism As shown in FIGS. 8 and 9 , the temperature zone switching driving mechanism of this embodiment includes a linear rail 41 and a linear driving mechanism for driving the bracket body 20 to move along the linear rail 41 .
  • the linear drive mechanism of this embodiment includes a first pulley 45 and a second pulley 46 respectively located at both ends of the linear track 41. A synchronous belt 47 is set between the first pulley 45 and the second pulley 46.
  • the linear drive mechanism Fixedly connected to the bracket body 20 , the linear drive mechanism also includes a drive motor 48 for driving the first pulley 45 or the second pulley 46 to rotate.
  • the drive motor 48 in this embodiment is a stepper motor.
  • the linear drive mechanism can also be implemented using various existing methods, which will not be described again.
  • the temperature control system of the PCR fluorescence quantitative analyzer of this embodiment also includes a device for driving the relative movement between the bracket body 20 and the support plate 51 in the vertical direction to cause the sample tube placed in the sample tube placement plate 21 to drop downward.
  • a switching drive mechanism that enters the hot well 54 or moves upward from the hot well 54 .
  • the function of the opening and closing driving mechanism is to adjust the height of the support plate 51 , that is, the opening and closing driving mechanism is a linear driving mechanism that adjusts the height in the vertical direction, and can be implemented in various existing ways.
  • the opening and closing driving mechanism includes a height adjustment plate 69 located below the support plate 51 , and the support plate 51 and the height adjustment plate 69 move synchronously.
  • the height adjustment plate 69 is provided with a screw nut 70, and the screw nut 70 is provided with a threaded screw 71 that matches its thread.
  • the opening and closing driving mechanism of this embodiment also includes a transmission mechanism connected to the threaded screw rod 71.
  • the height adjustment driving mechanism is dynamically connected to drive the threaded screw rod 71 to rotate.
  • the height adjustment driving mechanism of this embodiment includes a height adjustment motor 72.
  • the output shaft of the height adjustment motor 72 can be directly connected to the threaded screw rod 71, or can be adjusted in height.
  • a transmission mechanism such as a gear transmission mechanism, a chain transmission, etc., is provided between the output shaft of the motor 72 and the threaded screw rod 71 .
  • a belt transmission mechanism is provided between the output shaft of the height adjustment motor 72 and the threaded screw rod 71 .
  • the belt transmission mechanism includes pulleys respectively provided on the output shaft of the height adjustment motor 72 and the threaded screw rod 71 .
  • the transmission belts 73 on the two pulleys will not be described again.
  • the bracket body 20 is provided with opening and closing guide rods 22 respectively located on both sides of the sample tube placing plate 21.
  • the heating cover assembly slides with the opening and closing guide rods 22. When the heating cover assembly moves along the When the opening and closing guide rod 22 moves downward, it can be covered on the sample tube placing plate 21. When the hot cover assembly moves upward along the opening and closing guide rod 22, the sample tube placing plate 21 can be opened.
  • the hot cover module of this embodiment includes a bracket assembly, and also includes a device for driving the hot cover assembly to move along the opening and closing guide rod 22 to make the
  • the hot cover assembly covers the sample tube placement plate 21 or is a heat cover opening and closing mechanism opened from the sample tube placement plate 21 .
  • the hot cover opening and closing mechanism can be implemented in a variety of ways, such as directly providing a cylinder, an electric cylinder, a threaded screw mechanism, a gear rack mechanism, etc. on the bracket body 20 for driving the hot cover assembly to move along the opening and closing guide rod 22. .
  • the hot cover opening and closing mechanism of this embodiment includes a linear track 41 for guiding the movement of the bracket body 20 , a hot cover track 42 for guiding the movement of the hot cover assembly, and a linear drive for driving the bracket body 20 to move along the linear track 41 mechanism.
  • the linear guide assembly of this embodiment includes guide plates 49 located on both sides of the bracket body 20.
  • a bottom plate 49a is provided below the guide plate 49.
  • the linear track 41 is provided on the bottom plate 49a, and the heat cover track 42 is provided on the guide plate. 49 on.
  • guide plates 49 can also be provided only on both sides of the bracket body 20.
  • the hot cover track 42 includes a first heat cover track section 42a and a second heat cover track section 42b that are parallel to the linear track 41.
  • the distance between the first heat cover track section 42a and the linear track 41 is greater than the distance between the second heat cover track section 42b and the linear track 41.
  • spacing, a connecting section 42c is provided between the first section 42a of the hot cover track and the second section 42b of the hot cover track.
  • the linear drive mechanism drives the bracket body 20 to move along the linear track 41
  • the position of the hot cover assembly Moving between the first section 42a of the hot cover track and the second section 42b of the hot cover track, that is, the hot cover assembly follows the bracket body 20 along the linear track.
  • the track 41 moves and can move along the opening and closing guide rod 22 under the action of the hot cover track 42.
  • the heating cover assembly is located on the first section 42a of the heating cover track, the heating cover assembly is located above the sample tube placing plate 22, that is, the heating cover assembly is in an open state at this time; when the heating cover assembly is located on the second section 42b of the heating cover track, the heating cover assembly is located on the second section 42b of the heating cover track.
  • the cover assembly covers the sample tube placing plate 21, that is, the hot cover assembly is in a closed state at this time.
  • the hot cover assembly moves within the connection section 42c, the relationship between the hot cover assembly and the sample tube placement plate 21 is in an open or closed process change state.
  • the included angle between the connecting section 42c and the heated cover track section 42a is 110-150°
  • the included angle between the connecting section 42c and the heated cover track section 42a in this embodiment is 135°. , which can make the hot cover assembly run more smoothly between the first section 42a of the hot cover track and the second section 42b of the hot cover track.
  • a bracket position detection component for detecting the position of the bracket body 20 is also provided between the linear rail 41 and the bracket body 20 .
  • the bracket position detection component can be implemented using a variety of existing methods, such as displacement sensors.
  • the bracket position detection assembly of this embodiment includes first photocoupler sensors 43 respectively disposed at both ends of the linear track 41 .
  • the bracket body 20 is provided with a first light blocking plate 44 that cooperates with the first photocoupler sensor 43 .
  • the two first photocoupler sensors 43 respectively correspond to the first section 42a of the hot cover track and the second section 42b of the hot cover track.
  • the bracket body 20 is located at the first section 42a of the hot cover track or the second section 42b of the hot cover track.
  • On the second section 42b of the track it can be determined whether the hot cover assembly is covered on the sample tube placing plate 21.
  • the position of the bracket body 20 is detected by the bracket position detection component, so that the current temperature zone of the sample tube placement plate 21 can be determined based on the position of the bracket body 20, thereby realizing the detection of the sample tube placement plate 21.
  • the heating temperature of the sample within is monitored for technical purposes.
  • the linear track 41 of this embodiment is fixedly installed on the bottom plate 49a.
  • the linear track 49 is provided with a linear slider 41a that slides with it.
  • the bracket body 20 is fixedly connected to the linear slider 41a.
  • the transmission belt 47 in this embodiment is also fixedly connected to the linear slider 41a, that is, the transmission belt 47 is fixedly connected to the bracket body 20 through the linear slider 41a.
  • the hot cover track 42 is a guide groove opened on the guide plate 49 .
  • the hot cover assembly is provided with a guide shaft 17 that cooperates with the guide groove. To lighten the guide shaft 17 with the hot cover track 42.
  • a bearing 18 that is in rolling fit with the guide groove is installed on the guide shaft.
  • opening and closing is achieved by controlling the up and down movement of the hot cover assembly relative to the sample tube placing plate 21.
  • the hot cover assembly is always located above the sample tube placing plate 21, which may cause inconvenience in picking up and placing the sample tubes.
  • the stand body 20 of this embodiment is provided with an entry and exit bin rail 23 and a movable bracket 24 that slides in and out of the bin rail 23.
  • the sample tube placement plate 21 is installed on the movable bracket 24
  • the movable bracket 24 is driven by the in-and-out chamber driving mechanism to move along the in-out chamber track 23, so that the sample tube placement plate 21 can be extended from the bottom of the hot cover assembly, that is, the sample tube placement plate 21 and the hot cover assembly are misaligned to facilitate sample collection. Take and place the tube.
  • the sample tube placement plate 21 can also be driven to retract below the hot cover assembly.
  • the drive mechanism for entering and exiting the warehouse in this embodiment is essentially a linear drive mechanism, which can be implemented in a variety of existing ways, such as a rack and pinion mechanism, a threaded screw rod mechanism, a belt transmission mechanism, etc.
  • the entry and exit driving mechanism of this embodiment includes a threaded screw 25 parallel to the entry and exit track 23.
  • the movable bracket 24 is provided with a nut seat 26 that threadably matches the threaded screw 25.
  • the bracket body 20 is equipped with a screw for driving the threaded screw. 25 rotates the screw motor 27, that is, the entry and exit drive mechanism of this embodiment adopts a threaded screw mechanism.
  • an entry and exit bin position detection component for detecting the moving position of the movable bracket 24 is provided between the bracket body 20 and the movable bracket 24 in this embodiment.
  • the entry and exit position detection component can be implemented using a variety of existing methods, such as displacement sensors.
  • the bracket body 20 is provided with a second photocoupler sensor 28
  • the movable bracket 24 is provided with a second light blocking sheet 29 for cooperating with the second photocoupler sensor 28 .
  • the entrance and exit rails 23 and the linear rails 41 are parallel to each other.
  • a bracket heating film can also be provided in the bracket assembly, and a heat insulation structure can be configured at the same time.
  • the movable bracket 24 is equipped with a sample holder 30 that moves synchronously with it.
  • the sample tube placing plate 22 is installed on the sample holder 30.
  • the sample holder 30 is provided with a holder heating film located under the sample tube placing plate 22.
  • the sample Bracket 30 is made of heat-insulating material. In this way, the sample holder 30 is set separately on the movable stand 24 and the sample holder 30 is made of heat-insulating material, which can reduce the use of heat-insulating material, save costs, and at the same time meet the needs of sample heating and Placed for technical purposes of heat diffusion.
  • the thermal cover assembly of this embodiment includes a thermal cover 10 .
  • the hot cover 10 can be slidably matched with the opening and closing guide rod 22, and the guide shaft and bearing 18 can be arranged on the hot cover 10. That is, under the guidance of the hot cover track 42, the hot cover 10 can be directly driven to be placed relative to the sample tube.
  • the plate 21 moves to achieve opening and closing.
  • a rubber pad or the like can be provided on the bottom surface of the heat cover 10, which will not be described again.
  • the heating cover 10 can also be divided into a heating cover plate 14 and a heating cover insulation plate 15 located above the heating cover plate 14.
  • the heating cover plate 14 and the heating lid insulation plate There is a heating cover heating film between 15. The heat generated by the heating cover heating film heats the sample placed on the sample tube placement plate 21 through the heating cover cover plate 14.
  • the heat cover insulation plate 15 can also prevent the influence of heat diffusion. Other components.
  • the heat cover 10 is also provided with a temperature sensor 16 for measuring the temperature of the heat cover plate 14 and a second over-temperature protection switch provided on the heating circuit of the heat cover heating film, thereby achieving real-time monitoring of the temperature of the heat cover plate 14 Monitoring, when the temperature of the hot cover plate 14 is too high, the second over-temperature protection switch is used to automatically cut off the heating circuit to realize the over-heat protection function.
  • the heat cover insulation plate 15 can be slidably matched with the opening and closing guide rod 22, and the guide shaft and the bearing 18 can be arranged on the heat cover heat insulation plate 15.
  • the hot cover assembly also includes a heat cover upper frame 11 located above the heat cover 10.
  • a heat cover guide rod 12 is provided between the heat cover 10 and the heat cover upper frame 11.
  • One end of the rod 12 is fixedly connected to the heat cover 10, and the other end is in sliding fit with the heat cover upper frame 11.
  • the heat cover guide rod 12 is provided with a spring 13 between the heat cover 10 and the heat cover upper frame 11.
  • the upper frame 11 of the hot cover is provided with a linear bearing 19 that cooperates with the opening and closing guide rods 22.
  • the guide shaft and the bearing 18 are both arranged on the hot cover upper frame 11 , that is, the hot cover assembly of this embodiment cooperates with the hot cover track 42 through the hot cover upper frame 11 .
  • the hot cover 10 can be driven to move relative to the sample tube placing plate 21, that is, the hot cover 10 can be driven to cover the sample tube placing plate 21 or remove from the sample tube. Place plate 21 on to open.
  • the hot lid guide rod 12 By arranging the hot lid guide rod 12 between the hot lid upper frame 11 and the hot lid 10 and arranging the spring 13 on the hot lid guide rod 12, not only can rigid collision between the hot lid 10 and the sample tube placement plate 21 be avoided, but also When the hot lid is on the shelf 11 relative
  • the hot cover 10 can also be driven to continue to move downward and compress the spring 13, using the elastic force of the spring 13 to
  • the hot cover 10 is pressed and fixed on the sample tube placing plate 21, so that the hot cover 10 and the sample tube placing plate 21 can be pressed and fixed adaptively.
  • the sample tube placement plate 21 is placed horizontally, and the opening and closing guide rod 22 is located in the vertical direction. That is, in this embodiment, the hot cover guide rod 12 and the opening and closing guide rod 22 are parallel to each other, and the opening and closing guide rod 22 is parallel to each other.
  • the rod 22 and the linear track 41 are perpendicular to each other.
  • the photoelectric detection module 80 is provided with an optical fiber fixed disk 81 and a fluorescent component rotating disk 82.
  • the optical fiber fixed disk 81 is provided with an optical fiber receiving interface and an optical fiber excitation interface.
  • the hot well 54 is provided with two optical fiber connection ports. 63. There are optical fibers between the optical fiber receiving interface and the optical fiber excitation interface and the two optical fiber connection ports 63 respectively.
  • the fluorescent component rotating disk 82 is provided with a receiving barrel assembly 83, an excitation barrel assembly 84 and a conductive slip ring 85.
  • the optical fiber fixed disk 81 is provided with a motor 86 for driving the fluorescent component rotating disk 82 to rotate.
  • the optical fiber fixed disk 81 and the fluorescent A third photocoupler sensor 87 for detecting the rotational position of the fluorescent component rotating disk 82 is disposed between the component rotating disks 82 .
  • the specific implementation of the photoelectric detection module 80 can be found in the Chinese patent with patent number CN2021226983550 and the patent name "PCR Fluorescence Detection Module", which will not be described again.

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Abstract

本发明公开了一种全自动PCR荧光定量分析仪，包括机架，机架上设有温控系统和光电检测模块，温控系统包括热盖模块和设置在热盖模块下方的温控模块；温控模块包括支撑板，支撑板上并列设有至少两个调温模块；调温模块包括调温底座，调温底座上设有热井座，热井座上设有热井；调温底座与热井座之间设有用于控制热井内的温度的温控组件；热盖模块包括热盖组件和支架组件，支架组件包括支架主体，支架主体上安装有样本管放置板，热盖组件设置在样本管放置板的上方并与样本管放置板之间实现开合；温控系统还包括用于驱动支架主体与支撑板之间分别沿水平和竖直方向相对移动的温区切换驱动机构和分合驱动机构。

Description

全自动PCR荧光定量分析仪
本申请要求于2022年6月23日提交中国专利局、申请号为202210719808.X、申请名称为“全自动PCR荧光定量分析仪”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本发明属于医疗器械技术领域，具体的为一种全自动PCR荧光定量分析仪。
背景技术
PCR即聚合酶链式反应，是一种用于扩增检测特定DNA片段的分子生物学技术。DNA/RNA循环扩增时间的快慢决定了整机设备是否具有市场竞争力，扩增过程需要在不同的温度下实现DNA/RNA的解链和复制。
目前，现有的PCR荧光定量分析仪的温控模块均设为单一固定结构，即扩增过程需要的不同温度均在一个模块上通过升降温来实现，这样整个循环扩增的时间无法缩短，无法满足科研及临床诊断对快速扩增循环检测的需求。
另外，现有的PCR荧光定量分析仪采用转动电机和直线丝杆电机来驱动PCR热盖转动和锁紧，虽然在一定程度上能够满足PCR热盖自动翻转和锁紧的技术目的，但采用两个电机，存在结构复杂和不利于后期维护保养的问题。另外，现有的翻转式热盖还存在工作时需要的空间较大的问题，不利于分析仪内部空间布局设计。
发明内容
有鉴于此，本发明的目的在于提供一种全自动PCR荧光定量分析仪，通过设置多个温区以实现所需温度的快速切换，以满足快速扩增循环检测的需求。
为达到上述目的，本发明提供如下技术方案：
一种全自动PCR荧光定量分析仪，包括机架，所述机架上设有温控系统和光电检测模块，所述温控系统包括热盖模块和设置在所述热盖模块下方的温控模块；
所述温控模块包括支撑板，所述支撑板上并列设有至少两个调温模块；所述调温模块包括调温底座，所述调温底座上设有热井座，所述热井座上设有热井；所述调温底座与所述热井座之间设有用于控制所述热井内的温度的温控组件；
所述热盖模块包括热盖组件和支架组件，所述支架组件包括支架主体，所述支架主体上安装有样本管放置板，所述热盖组件设置在所述样本管放置板的上方并与所述样本管放置板之间实现开合；
所述温控系统还包括用于驱动所述支架主体与所述支撑板之间沿水平方向相对移动以使所述样本管放置板位于不同的所述调温模块正上方的温区切换驱动机构；
所述温控系统还包括用于驱动所述支架主体与所述支撑板之间沿竖直方向相对移动以使放置在所述样本管放置板内的样本管向下落入到所述热井内或从所述热井内向上移出的分合驱动机构；
所述热井上设有两个光纤连接口，所述光电检测模块上设有光纤固定盘，所述光纤固定盘上设有光纤接收接口和光纤激发接口，光纤接收接口和光纤激发接口与两个所述光纤连接口之间分别设有光纤。
进一步，所述温控组件包括用于控制温度升降的温控元件，所述温控元件与所述热井座之间设有第一导热层。
进一步，所述调温底座上设有散热片，所述调温底座与所述温控元件之间设有第二导热层。
进一步，所述支撑板的下方设有散热风机，所述散热片的两侧分别设有散热风道。
进一步，所述调温底座上设有用于定位所述热井座的热井定位块。
进一步，所述热井座上设有用于定位所述光纤的光纤定位座和将所述光纤压紧固定在所述光纤定位座上的光纤压板。
进一步，所述调温底座内设有用于安装温度传感器的安装孔，所述热井座内设有用于测量所述热井温度的热井温度传感器，所述温控组件 的供电电路上设有第一过温保护开关。
进一步，所述温区切换驱动机构包括直线轨道和用于驱动所述支架主体沿着所述直线轨道移动的直线驱动机构。
进一步，所述支架主体上设有分别位于所述样本管放置板两侧的开合导杆，所述热盖组件与所述开合导杆滑动配合；
所述直线轨道的上方设有用于导向所述热盖组件的热盖轨道，所述热盖轨道包括与所述直线轨道平行的热盖轨道一段和热盖轨道二段，所述热盖轨道一段与所述直线轨道之间的间距大于所述热盖轨道二段与所述直线轨道之间的间距，所述热盖轨道一段和热盖轨道二段之间设有连接段相连通；
当所述热盖组件位于所述热盖轨道一段上时，所述热盖组件位于所述样本管放置板的上方；当所述热盖组件位于所述热盖轨道二段上时，所述热盖组件盖在所述样本管放置板上。
进一步，所述支架主体与所述直线轨道之间设有用于检测所述支架主体在所述直线轨道上的移动位置的支架位置检测组件。
进一步，所述直线驱动机构包括分别位于所述直线轨道两端的第一带轮和第二带轮，所述第一带轮和第二带轮之间套设有同步带，所述同步带与所述支架主体固定连接，所述直线驱动机构还包括用于驱动所述第一带轮或第二带轮转动的驱动电机。
进一步，所述支架主体的两侧分别设有导向板，所述直线轨道和热盖轨道分别设置在所述导向板上；或，所述支架主体的两侧分别设有导向板，所述导向板的下方设有底板，所述直线轨道设置在所述底板上，所述热盖轨道设置在所述导向板上。
进一步，所述热盖轨道为开设在所述导向板上的导向槽，所述热盖组件上设有与所述导向槽配合的导向轴。
进一步，所述导向轴上安装有与所述导向槽滚动配合的轴承。
进一步，所述热盖组件上设有与所述开合导杆配合的直线轴承。
进一步，所述开合导杆与所述直线轨道之间相互垂直。
进一步，所述连接段与所述热盖轨道一段之间的夹角为110-150°。
进一步，所述支架主体上设有进出仓轨道和与所述进出仓轨道滑动 配合的活动支架，所述样本管放置板安装在所述活动支架上，所述支架主体上设有用于驱动所述活动支架沿着所述进出仓轨道移动以驱动所述样本管放置板从所述热盖组件下方伸出或缩回到所述热盖组件下方的进出仓驱动机构。
进一步，所述进出仓驱动机构包括与所述进出仓轨道平行的螺纹丝杆，所述活动支架上设有与所述螺纹丝杆螺纹配合的螺母座，所述支架主体上安装有用于驱动所述螺纹丝杆转动的丝杆电机。
进一步，所述活动支架与所述支架主体之间设有用于检测所述活动支架的移动位置的进出仓位置检测组件。
进一步，所述活动支架上安装有与其同步移动的样本支架，所述样本管放置板安装在所述样本支架上，所述样本支架上设有位于所述样本管放置板下方的支架加热膜，所述样本支架采用隔热材料制成。
进一步，所述分合驱动机构包括位于所述支撑板下方的高度调节板，所述支撑板与所述高度调节板同步移动；所述高度调节板上设有丝杆螺母，所述丝杆螺母内设有与其螺纹配合的螺纹丝杆；
还包括与所述螺纹丝杆传动连接以驱动所述螺纹丝杆转动的高度调节驱动机构。
进一步，所述热盖组件包括热盖和位于热盖上方的热盖上架，所述热盖与所述热盖上架之间设有热盖导杆，所述热盖导杆的一端与所述热盖固定连接、另一端与所述热盖上架之间滑动配合，所述热盖导杆上套装设有位于所述热盖与所述热盖上架之间的弹簧。
进一步，所述热盖包括热盖盖板和位于所述热盖盖板上方的热盖隔热板，所述热盖盖板与所述热盖隔热板之间设有热盖加热膜。
进一步，所述热盖还包括用于测量所述热盖盖板温度的温度传感器和设置在所述热盖加热膜的加热电路上的第二过温保护开关。
进一步，还包括电源模块和电控模块，所述电源模块位于所述电控模块的上方，且所述电控模块位于所述光电检测模块的上方。
进一步，还包括显示屏，所述显示屏位于所述热盖模块的上方。
本发明的有益效果在于：
本发明的全自动PCR荧光定量分析仪，通过在机架上安装温控系 统和光电检测组件，利用光电检测组件可直接对经温控系统循环扩增后的样本进行检测；其中，温控系统包括热盖模块和温控模块，通过在温度模块内设置至少两个调温模块，如此，在使用过程中，可以利用温控组件将对应热井内的温度调节为设定温度，如此，不同的调温模块形成不同的温区；而后利用温区切换驱动机构驱动支架主体与支撑板之间在水平方向相对移动，从而可以使样本管放置板位于不同调温模块的正上方，也即可使样本管放置板位于不同的温区，实现温区快速切换；利用分合驱动机构驱动支架主体与支撑板之间在竖直方向上相对移动，可使放置在样本管放置板上的样本管在切换到对应的温区后，驱动使样本管向下落入到对应的热井内，当需要切换温区时，驱动样本管从对应的热井上方移出，使样本管与热井之间分离，以便于温区切换。
本发明还具有以下技术效果：
1)通过在热盖开合机构内设置与支架主体滑动配合的直线轨道和与热盖上架配合的热盖轨道，当直线驱动机构驱动支架主体沿着直线轨道移动的过程中，可驱动样本进入不同的温区以实现热循环；在开合导杆的作用下，热盖组件也沿着与直线轨道平行的方向移动，同时在热盖轨道的导向作用下，热盖上架在热盖轨道一段和热盖轨道二段之间还沿着与直线轨道垂直的方向移动，即热盖上架在热盖轨道一段和热盖轨道二段之间可相对于支架主体沿着开合导杆向下或向上移动，以实现将热盖盖在样本管放置板上或将热盖从样本管放置板上打开的技术目的。
2)通过在支架主体上设置进出仓轨道，并将活动支架安装在进出仓轨道上，如此，利用进出仓驱动机构可驱动活动支架沿着进出仓轨道移动，由于样本管放置板安装在活动支架上，进而驱动样本管放置板沿着进出仓轨道移动，如此，可使样本管放置板从热盖的下方露出，以便于取放样本管，样本管取放完成后，又可使样本管放置板回到热盖下方。
3)通过在活动支架上设置样本支架，并在样本支架上设置位于样本管放置板下方的支架加热膜，可实现对放置在样本管放置板上的样本加热的技术目的，与热盖加热膜配合，加热效果更好；样本支架采用隔热材料制成，能够防止热量扩散影响其他元器件。
4)通过在支架主体上设置位于样本管放置板两侧的开合导杆，并 将热盖组件的热盖上架安装在开合导杆上并可沿着开合导杆移动，如此，驱动热盖上架相对于支架主体沿着开合导杆朝向下移动，即可将热盖盖在样本管放置板上，驱动热盖上架相对于支架主体沿着开合导杆朝上移动，即可将热盖从样本管放置板上打开；即热盖采用沿着开合导杆平移的方式与样本管放置板之间实现开合，相较于现有的铰链式开合方式，占用空间小且结构更加简单。
5)通过在热盖和热盖上架之间设置热盖导杆，并在热盖导杆上套装设置弹簧，如此，不仅能够避免热盖与样本管放置板之间发生刚性碰撞，而且当热盖上架相对于支架主体沿着开合导杆朝向下移动时，在热盖与样本管放置板接触后，还可驱动热盖继续向下移动并压缩弹簧，利用弹簧的弹力将热盖压紧固定在样本管放置板上，使热盖与样本管放置板之间能够自适应压紧固定。
附图说明
为了使本发明的目的、技术方案和有益效果更加清楚，本发明提供如下附图进行说明：
图1为本发明全自动PCR荧光定量分析仪实施例的结构示意图；
图2为本实施例全自动PCR荧光定量分析仪的立体图；
图3为温控系统的结构示意图；
图4为温控模块的结构示意图；
图5为温控模块的立体图；
图6为调温模块的结构示意图；
图7为调温模块的立体图；
图8为热盖模块实施例的右轴侧图；
图9为热盖模块的左轴侧图；
图10为导向板的结构示意图；
图11为支架组件的结构示意图；
图12为支架组件的轴侧图；
图13为热盖组件的结构示意图；
图14为光电检测模块的结构示意图。
1-样本管；
10-热盖；11-热盖上架；12-热盖导杆；13-弹簧；14-热盖盖板；15-热盖隔热板；16-温度传感器；17-导向轴；18-轴承；19-直线轴承；
20-支架主体；21-样本管放置板；22-开合导杆；23-进出仓轨道；24-活动支架；25-螺纹丝杆；26-螺母座；27-丝杆电机；28-第二光耦传感器；29-第二挡光片；30-样本支架；31-顶板；
40-热盖模块；41-直线轨道；42-热盖轨道；42a-热盖轨道一段；42b-热盖轨道二段；42c-连接段；43-第一光耦传感器；44-第一挡光片；45-第一带轮；46-第二带轮；47-同步带；48-驱动电机；49-导向板；49a-底板。
50-温控模块；51-支撑板；51a-低温区；51b-中文区；51c-高温区；52-调温底座；53-热井座；54-热井；55-温控元件；56-第一导热层；57-散热片；58-第二导热层；59-散热风机；60-散热风道；61-热井定位块；62-光纤；63-光纤连接口；64-光纤定位座；65-光纤压板；66-安装孔；67-热井温度传感器；68-过温保护开关；69-高度调节板；70-丝杆螺母；71-螺纹丝杆；72-高度调节电机；73-传动带；74-调温模块；75-热井座压板。
80-光电检测模块；81-固定光纤盘；82-荧光组件旋转盘；83-接收镜筒组件；84-激发镜筒组件；85-导电滑环；86-电机；87-第三光耦传感器；
100-机架；101-电源模块；102-电控模块；103-显示屏。
具体实施方式
下面结合附图和具体实施例对本发明作进一步说明，以使本领域的技术人员可以更好的理解本发明并能予以实施，但所举实施例不作为对本发明的限定。
如图1和图2所示，本实施例的全自动PCR荧光定量分析仪，包括机架100，机架100上设有温控系统、光电检测模块80、电源模块101、电控模块102和显示屏103。具体的，电源模块101位于电控模块102的上方，且电控模块102位于光电检测模块80的上方，显示屏103位 于温控系统的上方。如图3所示，本实施例的温控系统包括热盖模块40和设置在热盖模块40下方的温控模块50，显示屏103位于热盖模块40的上方。
具体的，如图4-5所示，本实施例的温控模块包括支撑板51，支撑板51上并列设有至少两个调温模块74。本实施例的调温模块74设为3个，使用中，分别控制3个调温模块的温度，使3个调温模块依次形成低温区51a、中温区51b和高温区51c。具体的，调温模块74的数量可以根据实际需要进行设置，如将调温模块74设为2个、4个或4个以上等，不再累述。具体的，如图6和图7所示，本实施例的调温模块包括调温底座52，调温底座52上设有热井座53，热井座53上设有热井54。调温底座52与热井座53之间设有用于控制热井54内温度的温控组件，利用温控组件可调节热井座53的温度，进而调节热井54的温度。温控组件主要起到调节温度的作用，可以采用现有的多种方式实现，本实施例中，温控组件包括用于控制温度升降的温控元件55，温控元件55与热井座53之间设有第一导热层56，温控元件55调节温度，并利用第一导热层56将热量快速传递至热井座53，以实现热井座53温度的快速调节。具体的，本实施例的调温底座52上设有散热片57，调温底座52与温控元件55之间设有第二导热层58，当需要散热时，热量还可通过第二导热层58快速传递至散热片57以实现散热降温。具体的，本实施例的温控元件55采用半导体制冷器，第一导热层56和第二导热层58均采用石墨烯材料制成。为了加快散热速度，本实施例在支撑板51的下方设置散热风机59，在散热片57的两侧还分别设有散热风道60。具体的，本实施例中，分别位于三个调温模块两侧的散热风道60设为一体。优选的，本实施例的调温底座52上设有用于定位热井座53的热井定位块61，以实现热井座53定位安装，以便于与放置在样本管放置板21内的样本管之间实现对接，另外，本实施例的热井座53通过热井座压板75固定安装在调温底座52上。如图6所示，本实施例的调温底座52内设有用于安装温度传感器的安装孔66，热井座53内设有用于测量热井54温度的热井温度传感器67，温控组件的供电电路上设有第一过温保护开关68。
进一步，如图6-7所示，本实施例的热井54上设有用于连接光纤62的光纤连接口63，热井座53上设有用于定位光纤62的光纤定位座64和将光纤62压紧固定在光纤定位座64上的光纤压板65，以提高与光纤62之间的连接可靠性。当然，在一些实施例中，可以仅在位于高温区51c和中温区51b的调温模块的热井73上设置光纤连接口63，在另外一些实施例中，也可以在所有调温模块的热井73上设置光纤连接口63，本实施例仅在位于高温区51c和中温区51b的调温模块的热井73上设置光纤连接口63。
如图8所示，本实施例的热盖模块包括热盖组件和支架组件，支架组件包括支架主体20，支架主体20上安装有样本管放置板21，热盖组件设置在样本管放置板21的上方并与样本管放置板21之间实现开合。
具体的，本实施例的PCR荧光定量分析仪温控系统还包括用于驱动支架主体20与支撑板51之间沿水平方向相对移动以使样本管放置板21位于不同的调温模块正上方的温区切换驱动机构。如图8和图9所示，本实施例的温区切换驱动机构包括直线轨道41和用于驱动支架主体20沿着直线轨道41移动的直线驱动机构。本实施例的直线驱动机构包括分别位于直线轨道41两端的第一带轮45和第二带轮46，第一带轮45和第二带轮46之间套设有同步带47，同步带47与支架主体20固定连接，直线驱动机构还包括用于驱动第一带轮45或第二带轮46转动的驱动电机48，本实施例的驱动电机48采用步进电机。当然，直线驱动机构还可以采用现有的多种方式实现，不再累述。
具体的，本实施例的PCR荧光定量分析仪温控系统还包括用于驱动支架主体20与支撑板51之间沿竖直方向相对移动以使放置在样本管放置板21内的样本管向下落入到热井54内或从热井54内向上移出的分合驱动机构。分合驱动机构的作用为调节支撑板51的高度，即分合驱动机构是一种调节竖直方向上高度的直线驱动机构，可以采用现有的多种方式实现。本实施例中，分合驱动机构包括位于支撑板51下方的高度调节板69，支撑板51与高度调节板69同步移动。高度调节板69上设有丝杆螺母70，丝杆螺母70内设有与其螺纹配合的螺纹丝杆71。如图4和图5所示，本实施例的分合驱动机构还包括与螺纹丝杆71传 动连接以驱动螺纹丝杆71转动的高度调节驱动机构，本实施例的高度调节驱动机构包括高度调节电机72，高度调节电机72的输出轴可以直接与螺纹丝杆71相连，也可以在高度调节电机72的输出轴与螺纹丝杆71之间设置传动机构，如齿轮传动机构、链传动等。本实施例在高度调节电机72的输出轴与螺纹丝杆71之间设有带传动机构，带传动机构包括分别设置在高度调节电机72的输出轴以及螺纹丝杆71上的带轮和套装在两个带轮上的传动带73，不再累述。
进一步，如图8和图9所示，支架主体20上设有分别位于样本管放置板21两侧的开合导杆22，热盖组件与开合导杆22滑动配合，当热盖组件沿着开合导杆22向下移动时，可盖在样本管放置板21上，当热盖组件沿着开合导杆22向上移动时，可打开样本管放置板21。为了驱动热盖组件沿着开合导杆22相对于样本管放置板21移动，本实施例的热盖模块包括支架组件，还包括用于驱动热盖组件沿着开合导杆22移动以使热盖组件盖在样本管放置板21上或从样本管放置板21上打开的热盖开合机构。热盖开合机构可以采用多种方式实现，如直接在支架主体20上设置用于驱动热盖组件沿着开合导杆22移动的气缸、电动缸、螺纹丝杆机构以及齿轮齿条机构等。本实施例的热盖开合机构包括用于导向支架主体20移动的直线轨道41、用于导向热盖组件移动的热盖轨道42和用于驱动支架主体20沿着直线轨道41移动的直线驱动机构。具体的，本实施例的直线导向组件包括分别位于支架主体20两侧的导向板49，导向板49的下方设有底板49a，直线轨道41设置在底板49a上，热盖轨道42设置在导向板49上。当然，在其他一些实施例中，也可以仅在支架主体20的两侧设置导向板49，此时可以将直线轨道41和热盖轨道42分别设置在导向板49上，如图8和图9所示。热盖轨道42包括与直线轨道41平行的热盖轨道一段42a和热盖轨道二段42b，热盖轨道一段42a与直线轨道41之间的间距大于热盖轨道二段42b与直线轨道41之间的间距，热盖轨道一段42a和热盖轨道二段42b之间设有连接段42c相连通，如此，当直线驱动机构驱动支架主体20沿着直线轨道41移动的过程中，热盖组件的位置在热盖轨道一段42a和热盖轨道二段42b之间移动，即热盖组件既随着支架主体20沿着直线轨 道41移动，又在热盖轨道42的作用下可沿着开合导杆22移动。当热盖组件位于热盖轨道一段42a上时，热盖组件位于样本管放置板22的上方，即此时热盖组件处于打开状态；当热盖组件位于热盖轨道二段42b上时，热盖组件盖在样本管放置板21上，即此时热盖组件处于关闭状态。当热盖组件在连接段42c内移动使，热盖组件与样本管放置板21之间处于打开或关闭的过程变化状态。在本实施例的优选方案中，连接段42c与热盖轨道一段42a之间的夹角为110-150°，本实施例的连接段42c与热盖轨道一段42a之间的夹角为135°，可使热盖组件在热盖轨道一段42a和热盖轨道二段42b之间的运行更加平稳。当连接段42c与热盖轨道一段42a之间的夹角小于110°时，会导致热盖组件在竖直方向上运动困难，增加电机负荷；当连接段42c与热盖轨道一段42a之间的夹角大于150°时，会增加热盖组件在水平方向上的运动行程。
如图9所示，为了检测支架主体20沿着直线轨道41移动的位置，还在直线轨道41与支架主体20之间设置用于检测支架主体20位置的支架位置检测组件。支架位置检测组件可以采用现有的多种方式实现，如位移传感器等。本实施例的支架位置检测组件包括分别设置在直线轨道41的两端的第一光耦传感器43，支架主体20上设有与第一光耦传感器43配合的第一挡光片44。两个第一光耦传感器43分别与热盖轨道一段42a和热盖轨道二段42b对应，如此，通过两个第一光耦传感器43，可判断支架主体20位于热盖轨道一段42a或热盖轨道二段42b上，从而可以判断热盖组件是否盖在样本管放置板21上。当然，本实施例中，通过支架位置检测组件检测支架主体20的位置，从而还可以根据支架主体20的位置来判断样本管放置板21当前所处的温区，从而实现对样本管放置板21内的样本加热温度进行监控的技术目的。
如图10所示，本实施例的直线轨道41固定安装在底板49a上，直线轨道49上设有与其滑动配合的直线滑块41a，支架主体20与直线滑块41a固定连接。当然，本实施例的传动带47也与直线滑块41a固定连接，即传动带47通过直线滑块41a与支架主体20固定连接。本实施例中，热盖轨道42为开设在导向板49上的导向槽。如图9所示，热盖组件上设有与导向槽配合的导向轴17。为了减轻导向轴17与热盖轨道 42之间的摩擦力，本实施例在导向轴上安装有与导向槽滚动配合的轴承18。
由于本实施例中，通过控制热盖组件相对于样本管放置板21上下移动的方式来实现开合，如此，热盖组件始终位于样本管放置板21的上方，可能会造成样本管取放不便。有鉴于此，如图11和图12所示，本实施例的支架主体20上设有进出仓轨道23和与进出仓轨道23滑动配合的活动支架24，样本管放置板21安装在活动支架24上，支架主体20上设有用于驱动活动支架24沿着进出仓轨道23移动以驱动样本管放置板21从热盖组件下方伸出或缩回到热盖组件下方的进出仓驱动机构。如此，利用进出仓驱动机构驱动活动支架24沿着进出仓轨道23移动，可以使样本管放置板21从热盖组件下方伸出，即样本管放置板21与热盖组件之间错位以便于样本管取放。当然，在样本管取放完成后，还可驱动样本管放置板21缩回到热盖组件下方。本实施例的进出仓驱动机构实质上是一个直线驱动机构，可以采用现有的多种方式实现，如齿轮齿条机构、螺纹丝杆机构、带传动机构等。本实施例的进出仓驱动机构包括与进出仓轨道23平行的螺纹丝杆25，活动支架24上设有与螺纹丝杆25螺纹配合的螺母座26，支架主体20上安装有用于驱动螺纹丝杆25转动的丝杆电机27，即本实施例的进出仓驱动机构采用螺纹丝杆机构。为了检测活动支架24沿着进出仓轨道23移动的位置，本实施例的支架主体20与活动支架24之间设有用于检测活动支架24移动位置的进出仓位置检测组件。进出仓位置检测组件可以采用现有的多种方式实现，如位移传感器等。本实施例的支架主体20上设有第二光耦传感器28，活动支架24上设有用于与第二光耦传感器28配合的第二挡光片29。第二光耦传感器28设为两个，两个第二光耦传感器28分别用于检测样本管放置板21出仓和进仓两个位置状态。本实施例的进出仓轨道23与直线轨道41之间相互平行。
进一步，位于提高对样本的加热效果，还可以在支架组件内设置支架加热膜，并同时配置隔热结构。本实施例中，活动支架24上安装有与其同步移动的样本支架30，样本管放置板22安装在样本支架30上，样本支架30上设有位于样本管放置板22下方的支架加热膜，样本支架 30采用隔热材料制成，如此，在活动支架24上单独设置样本支架30并将样本支架30采用隔热材料制成，可减少隔热材料的使用量，节省成本，同时满足对样本加热和放置热量扩散的技术目的。
具体的，如图13所示，本实施例的热盖组件，包括热盖10。此时可以使热盖10与开合导杆22滑动配合，将导向轴和轴承18设置在热盖10上，即在热盖轨道42的导向作用下，直接驱动热盖10相对于样本管放置板21移动以实现开合，当然，为了避免热盖10与样本管放置板21之间刚性碰撞，可以在热盖10的底面上设置橡胶垫等，不再累述。为了提高对样本的加热效果，还可将热盖10分体设置为热盖盖板14和位于热盖盖板14上方的热盖隔热板15，热盖盖板14与热盖隔热板15之间设有热盖加热膜，热盖加热膜产生的热量通过热盖盖板14对放置在样本管放置板21上的样本实现加热，同时热盖隔热板15还可以防止热量扩散影响其他元器件。热盖10内还设有用于测量热盖盖板14温度的温度传感器16和设置在热盖加热膜的加热电路上的第二过温保护开关，从而实现对热盖盖板14的温度的实时监测，当热盖盖板14的温度过高时，利用第二过温保护开关自动切断加热电路，实现过热保护功能。此时可以使热盖隔热板15与开合导杆22滑动配合，将导向轴和轴承18设置在热盖隔热板15上。
当然，如图13所示，本实施例中，热盖组件还包括位于热盖10上方的热盖上架11，热盖10与热盖上架11之间设有热盖导杆12，热盖导杆12的一端与热盖10固定连接、另一端与热盖上架11之间滑动配合，热盖导杆12上套装设有位于热盖10与热盖上架11之间的弹簧13，本实施例中，热盖上架11与两根开合导杆22之间滑动配合，热盖上架11上设有与开合导杆22配合的直线轴承19。导向轴和轴承18均设置在热盖上架11上，即本实施例的热盖组件通过热盖上架11与热盖轨道42配合。在热盖上架11沿着开合导杆12移动的带动作用下，可驱动热盖10相对于样本管放置板21移动，即可驱动热盖10盖在样本管放置板21上或从样本管放置板21上打开。通过在热盖上架11与热盖10之间设置热盖导杆12，并在热盖导杆12上设置弹簧13，不仅能够避免热盖10与样本管放置板21之间发生刚性碰撞，而且当热盖上架11相对 于支架主体20沿着开合导杆22朝向下移动时，在热盖10与样本管放置板21接触后，还可驱动热盖10继续向下移动并压缩弹簧13，利用弹簧13的弹力将热盖10压紧固定在样本管放置板21上，使热盖10与样本管放置板21之间能够自适应压紧固定。本实施例中，样本管放置板21水平放置，开合导杆22位于竖直方向上，即本实施例中，热盖导杆12与开合导杆22之间相互平行，且开合导杆22与直线轨道41之间相互垂直。
如图14所示，光电检测模块80上设有光纤固定盘81和荧光组件旋转盘82，光纤固定盘81上设有光纤接收接口和光纤激发接口，热井54上设有两个光纤连接口63，光纤接收接口和光纤激发接口与两个光纤连接口63之间分别设有光纤。荧光组件旋转盘82上设有接收镜筒组件83、激发镜筒组件84和导电滑环85，光纤固定盘81上设有用于驱动荧光组件旋转盘82转动的电机86，光纤固定盘81和荧光组件旋转盘82之间设有用于检测荧光组件旋转盘82转动位置的第三光耦传感器87。具体的，光电检测模块80的具体实施方式可以参见专利号为CN2021226983550，专利名称为“PCR荧光检测模块”的中国专利，不再累述。
以上实施例仅是为充分说明本发明而所举的较佳的实施例，本发明的保护范围不限于此。本技术领域的技术人员在本发明基础上所作的等同替代或变换，均在本发明的保护范围之内。本发明的保护范围以权利要求书为准。

Claims (27)

  1. 一种全自动PCR荧光定量分析仪，其特征在于：包括机架(100)，所述机架(100)上设有温控系统和光电检测模块(80)，所述温控系统包括热盖模块(40)和设置在所述热盖模块(40)下方的温控模块(50)；
    所述温控模块(50)包括支撑板(51)，所述支撑板(51)上并列设有至少两个调温模块(74)；所述调温模块(74)包括调温底座(52)，所述调温底座(52)上设有热井座(53)，所述热井座(53)上设有热井(54)；所述调温底座(52)与所述热井座(53)之间设有用于控制所述热井(54)内的温度的温控组件；
    所述热盖模块包括热盖组件和支架组件，所述支架组件包括支架主体(20)，所述支架主体(20)上安装有样本管放置板(21)，所述热盖组件设置在所述样本管放置板(21)的上方并与所述样本管放置板(21)之间实现开合；
    所述温控系统还包括用于驱动所述支架主体(20)与所述支撑板(51)之间沿水平方向相对移动以使所述样本管放置板(21)位于不同的所述调温模块正上方的温区切换驱动机构；
    所述温控系统还包括用于驱动所述支架主体(20)与所述支撑板(51)之间沿竖直方向相对移动以使放置在所述样本管放置板(21)内的样本管向下落入到所述热井(54)内或从所述热井(54)内向上移出的分合驱动机构；
    所述热井(54)上设有两个光纤连接口(63)，所述光电检测模块(80)上设有光纤固定盘(81)，所述光纤固定盘(81)上设有光纤接收接口和光纤激发接口，光纤接收接口和光纤激发接口与两个所述光纤连接口(63)之间分别设有光纤(62)。
  2. 根据权利要求1所述的全自动PCR荧光定量分析仪，其特征在于：所述温控组件包括用于控制温度升降的温控元件(55)，所述温控元件(55)与所述热井座(53)之间设有第一导热层(56)。
  3. 根据权利要求2所述的全自动PCR荧光定量分析仪，其特征在于：所述调温底座(52)上设有散热片(57)，所述调温底座(52)与所述温控元件(55)之间设有第二导热层(58)。
  4. 根据权利要求3所述的全自动PCR荧光定量分析仪，其特征在于：所述支撑板(51)的下方设有散热风机(59)，所述散热片(57)的两 侧分别设有散热风道(60)。
  5. 根据权利要求1所述的全自动PCR荧光定量分析仪，其特征在于：所述调温底座(52)上设有用于定位所述热井座(53)的热井定位块(61)。
  6. 根据权利要求1所述的全自动PCR荧光定量分析仪，其特征在于：所述热井座(53)上设有用于定位所述光纤(62)的光纤定位座(64)和将所述光纤(62)压紧固定在所述光纤定位座(64)上的光纤压板(65)。
  7. 根据权利要求1所述的全自动PCR荧光定量分析仪，其特征在于：所述调温底座(52)内设有用于安装温度传感器的安装孔(66)，所述热井座(53)内设有用于测量所述热井(54)温度的热井温度传感器(67)，所述温控组件的供电电路上设有第一过温保护开关(68)。
  8. 根据权利要求1所述的全自动PCR荧光定量分析仪，其特征在于：所述温区切换驱动机构包括直线轨道(41)和用于驱动所述支架主体(20)沿着所述直线轨道(41)移动的直线驱动机构。
  9. 根据权利要求8所述的全自动PCR荧光定量分析仪，其特征在于：所述支架主体(20)上设有分别位于所述样本管放置板(21)两侧的开合导杆(22)，所述热盖组件与所述开合导杆(22)滑动配合；
    所述直线轨道(41)的上方设有用于导向所述热盖组件的热盖轨道(42)，所述热盖轨道(42)包括与所述直线轨道(41)平行的热盖轨道一段(42a)和热盖轨道二段(42b)，所述热盖轨道一段(42a)与所述直线轨道(41)之间的间距大于所述热盖轨道二段(42b)与所述直线轨道(41)之间的间距，所述热盖轨道一段(42a)和热盖轨道二段(42b)之间设有连接段(42c)相连通；
    当所述热盖组件位于所述热盖轨道一段(42a)上时，所述热盖组件位于所述样本管放置板(22)的上方；当所述热盖组件位于所述热盖轨道二段(42b)上时，所述热盖组件盖在所述样本管放置板(21)上。
  10. 根据权利要求8所述的全自动PCR荧光定量分析仪，其特征在于：所述支架主体(20)与所述直线轨道(41)之间设有用于检测所述支架主体(20)在所述直线轨道(41)上的移动位置的支架位置检测组件。
  11. 根据权利要求8所述的全自动PCR荧光定量分析仪，其特征在于：所述直线驱动机构包括分别位于所述直线轨道(41)两端的第一带 轮(45)和第二带轮(46)，所述第一带轮(45)和第二带轮(46)之间套设有同步带(47)，所述同步带(47)与所述支架主体(20)固定连接，所述直线驱动机构还包括用于驱动所述第一带轮(45)或第二带轮(46)转动的驱动电机(48)。
  12. 根据权利要求9所述的全自动PCR荧光定量分析仪，其特征在于：所述支架主体(20)的两侧分别设有导向板(49)，所述直线轨道(41)和热盖轨道(42)分别设置在所述导向板(49)上；或，所述支架主体(20)的两侧分别设有导向板(49)，所述导向板的下方设有底板，所述直线轨道(41)设置在所述底板上，所述热盖轨道(42)设置在所述导向板(49)上。
  13. 根据权利要求12所述的全自动PCR荧光定量分析仪，其特征在于：所述热盖轨道(42)为开设在所述导向板(49)上的导向槽，所述热盖组件上设有与所述导向槽配合的导向轴。
  14. 根据权利要求13所述的全自动PCR荧光定量分析仪，其特征在于：所述导向轴上安装有与所述导向槽滚动配合的轴承(18)。
  15. 根据权利要求9所述的全自动PCR荧光定量分析仪，其特征在于：所述热盖组件上设有与所述开合导杆(22)配合的直线轴承(19)。
  16. 根据权利要求9所述的全自动PCR荧光定量分析仪，其特征在于：所述开合导杆(22)与所述直线轨道(41)之间相互垂直。
  17. 根据权利要求9所述的全自动PCR荧光定量分析仪，其特征在于：所述连接段(42c)与所述热盖轨道一段(42a)之间的夹角为110-150°。
  18. 根据权利要求1-17任一项所述的全自动PCR荧光定量分析仪，其特征在于：所述支架主体(20)上设有进出仓轨道(23)和与所述进出仓轨道(23)滑动配合的活动支架(24)，所述样本管放置板(21)安装在所述活动支架(24)上，所述支架主体(20)上设有用于驱动所述活动支架(24)沿着所述进出仓轨道(23)移动以驱动所述样本管放置板(21)从所述热盖组件下方伸出或缩回到所述热盖组件下方的进出仓驱动机构。
  19. 根据权利要求18所述的全自动PCR荧光定量分析仪，其特征在于：所述进出仓驱动机构包括与所述进出仓轨道(23)平行的螺纹丝杆(25)，所述活动支架(24)上设有与所述螺纹丝杆(25)螺纹配合的 螺母座(26)，所述支架主体(20)上安装有用于驱动所述螺纹丝杆(25)转动的丝杆电机(27)。
  20. 根据权利要求19所述的全自动PCR荧光定量分析仪，其特征在于：所述活动支架(24)与所述支架主体(20)之间设有用于检测所述活动支架(24)的移动位置的进出仓位置检测组件。
  21. 根据权利要求18所述的全自动PCR荧光定量分析仪，其特征在于：所述活动支架(24)上安装有与其同步移动的样本支架(30)，所述样本管放置板(22)安装在所述样本支架(30)上，所述样本支架(30)上设有位于所述样本管放置板(22)下方的支架加热膜，所述样本支架(30)采用隔热材料制成。
  22. 根据权利要求1所述的全自动PCR荧光定量分析仪，其特征在于：所述分合驱动机构包括位于所述支撑板(51)下方的高度调节板(69)，所述支撑板(51)与所述高度调节板(69)同步移动；所述高度调节板(69)上设有丝杆螺母(70)，所述丝杆螺母(70)内设有与其螺纹配合的螺纹丝杆(71)；
    还包括与所述螺纹丝杆(71)传动连接以驱动苏搜狐螺纹丝杆(71)转动的高度调节驱动机构。
  23. 根据权利要求1所述的全自动PCR荧光定量分析仪，其特征在于：所述热盖组件包括热盖(10)和位于热盖(10)上方的热盖上架(11)，所述热盖(10)与所述热盖上架(11)之间设有热盖导杆(12)，所述热盖导杆(12)的一端与所述热盖(10)固定连接、另一端与所述热盖上架(11)之间滑动配合，所述热盖导杆(12)上套装设有位于所述热盖(10)与所述热盖上架(11)之间的弹簧(13)。
  24. 根据权利要求23所述的全自动PCR荧光定量分析仪，其特征在于：所述热盖(10)包括热盖盖板(14)和位于所述热盖盖板(14)上方的热盖隔热板(15)，所述热盖盖板(14)与所述热盖隔热板(15)之间设有热盖加热膜。
  25. 根据权利要求24所述的全自动PCR荧光定量分析仪，其特征在于：所述热盖还包括用于测量所述热盖盖板(14)温度的温度传感器(16)和设置在所述热盖加热膜的加热电路上的第二过温保护开关。
  26. 根据权利要求1所述的全自动PCR荧光定量分析仪，其特征在于：还包括电源模块(101)和电控模块(102)，所述电源模块(101) 位于所述电控模块(102)的上方，且所述电控模块(102)位于所述光电检测模块(80)的上方。
  27. 根据权利要求1所述的全自动PCR荧光定量分析仪，其特征在于：还包括显示屏(103)，所述显示屏(103)位于所述热盖模块(40)的上方。
PCT/CN2023/082323 2022-06-23 2023-03-17 全自动pcr荧光定量分析仪 WO2023246199A1 (zh)

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