WO2023239627A3 - Methods for recombinant aav production - Google Patents

Methods for recombinant aav production Download PDF

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Publication number
WO2023239627A3
WO2023239627A3 PCT/US2023/024392 US2023024392W WO2023239627A3 WO 2023239627 A3 WO2023239627 A3 WO 2023239627A3 US 2023024392 W US2023024392 W US 2023024392W WO 2023239627 A3 WO2023239627 A3 WO 2023239627A3
Authority
WO
WIPO (PCT)
Prior art keywords
raav
recombinant aav
particles
methods
producing
Prior art date
Application number
PCT/US2023/024392
Other languages
French (fr)
Other versions
WO2023239627A2 (en
Inventor
Ping Liu
Ayda MAYER
Jie Li
Joseph M. SCARROTT
Yusuf B. JOHARI
Thilo H. POHLE
David C. James
Original Assignee
Regenxbio Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Regenxbio Inc. filed Critical Regenxbio Inc.
Publication of WO2023239627A2 publication Critical patent/WO2023239627A2/en
Publication of WO2023239627A3 publication Critical patent/WO2023239627A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Provided herein are improved methods for producing recombinant Adeno-Associated Virus (rAAV) particles. In some embodiments, a method for producing recombinant AAV (rAAV) particles provided herein comprises culturing cells capable of producing rAAV particles in the presence of an anti-mitotic agent, e.g., nocodazole. In some embodiments, a method for producing recombinant AAV (rAAV) particles provided herein comprises culturing cells capable of producing rAAV particles in the presence of a caspase inhibitor.
PCT/US2023/024392 2022-06-08 2023-06-05 Methods for recombinant aav production WO2023239627A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263350257P 2022-06-08 2022-06-08
US63/350,257 2022-06-08

Publications (2)

Publication Number Publication Date
WO2023239627A2 WO2023239627A2 (en) 2023-12-14
WO2023239627A3 true WO2023239627A3 (en) 2024-01-18

Family

ID=87060613

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/024392 WO2023239627A2 (en) 2022-06-08 2023-06-05 Methods for recombinant aav production

Country Status (1)

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WO (1) WO2023239627A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024081927A1 (en) * 2022-10-14 2024-04-18 Tenaya Therapeutics, Inc. Compounds for improvement of viral production

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WO2021221956A1 (en) * 2020-04-27 2021-11-04 The Regents Of The University Of California Compositions and methods for production of recombinant adeno-associated virus
WO2023053899A1 (en) * 2021-10-01 2023-04-06 株式会社カネカ Agent for inducing viral vector production
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