WO2023219485A1 - Mixture, agricultural product and method to protect plant embryos under extreme climate conditions - Google Patents

Mixture, agricultural product and method to protect plant embryos under extreme climate conditions Download PDF

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Publication number
WO2023219485A1
WO2023219485A1 PCT/MX2023/050018 MX2023050018W WO2023219485A1 WO 2023219485 A1 WO2023219485 A1 WO 2023219485A1 MX 2023050018 W MX2023050018 W MX 2023050018W WO 2023219485 A1 WO2023219485 A1 WO 2023219485A1
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mixture
strain
agricultural product
ars
plant
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PCT/MX2023/050018
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Spanish (es)
French (fr)
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Luis Rodrigo BECERRA CARRANZA
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Becerra Carranza Luis Rodrigo
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Publication of WO2023219485A1 publication Critical patent/WO2023219485A1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma

Definitions

  • the present invention is related to the technical field of Biotechnology and Agriculture, since it provides a mixture, an agricultural product and a method to protect embryos of plant seeds intended for sowing, against extreme conditions of temperature and humidity.
  • Treating vegetable seeds for planting crops with inappropriate pesticides or at high doses can affect the germination percentage or germination-emergence time.
  • Plant seed species and varieties differ in susceptibility; For example, legumes are more susceptible than cereals and among cereals, maize and wheat are more susceptible than barley (Castillo-Tovar 2015).
  • Patent document EP2019190758A discloses combinations of active ingredients, in particular a fungicidal composition, which comprise: (A) fluoxapiproline and (B) at least one biological control agent and/or (C) at least one compound selected from insecticides, acaricides and nematicides.
  • the compositions are useful for controlling especially harmful microorganisms, in particular oomycetes, in crop protection, for the control of animal pests and as plant growth regulators.
  • the biological control agents these can be selected from a large number of microorganisms, such as antibacterial agents and biological control agents that have an effect to improve the growth and/or health of plants; and these may be of the genus Bacillus spp.
  • Patent document WO2014095994A1 discloses compositions comprising a trizole compound; a component of respiration inhibitors or inhibitors of groups II, III; nucleic acid synthesis inhibitors; inhibitors of cell division and cytoskeleton; etc.; and a wide range of microbial biopesticides, without specifying their strains. As can be noted, this document discloses very complex compositions, where the majority of its components are chemical.
  • the patent document WO2015IB52771A refers to the combination of new nitrification inhibitors of formula I and biopesticides, as well as the combination of (thio) phosphoric acid triamides and biopesticides. Besides, refers to the use of this combination of new nitrification inhibitors and biopesticides to improve the health of a plant, and to the use of the combination of (thio)phosphoric acid triamide(s) and biopesticides to improve the health of a plant ; as well as compositions comprising the nitrification inhibitor or (T) and a biopesticide, and compositions comprising the triamide(s) of (thio) phosphoric acid and a biopesticide.
  • compositions of this patent document are very complex, where most of their components are chemical.
  • compositions or products disclosed in the aforementioned documents have the purpose of protecting plant embryos in conditions of high temperatures and humidity.
  • Figure 1 is a graph that illustrates the percentage of germination of seeds of two varieties of corn, treated with the probiotic + phytohormones mixture of the present invention; and that they were subjected to high temperatures.
  • the first object of the present invention is a mixture that contains: i) an isolated strain of Trichoderma harzianum, which is the ARS TH-19 strain, deposited with the deposit number CM-CNRG TB174; ii) an isolated strain of Bacillus subtilis which is the ARS BS-19 strain, deposited with the deposit number CM-CNRG TB175; iii) an isolated strain of Azospirillum brasilense, which is the ARS AB-19 strain, deposited with the deposit number CM-CNRG TB176; iv) an isolated strain of Metarhizium anisopliae; which is the RVP MA-20 strain, deposited with the deposit number CM-CNRG TB195; v) an isolated strain of Beauveria bassiana, which is the RVP BB-20 strain, deposited with the deposit number CM-CNRG TB196; and vi) a mixture of indoleacetic acid and gibberellic acid, which was observed to have positive effects for the protection of the
  • a second object of the present invention is an agricultural product for protecting a plant embryo against extreme conditions of temperatures and humidity, where said agricultural product comprises the mixture in accordance with the present invention.
  • a third object of this invention is a method for protecting a plant embryo from extreme conditions of temperature and humidity, which comprises applying an effective amount of the agricultural product in accordance with the present invention, to plant seeds intended for sowing. .
  • Method of the present invention is when the effective amount of the agricultural product is 1 mL per 1 Kg of seeds.
  • a preferred embodiment of said method is when the protection is for an embryo of plants of the Poaceae family.
  • Example 1 Evaluate the conservation of the germination capacity of the embryo of corn seeds, after being treated with a probiotic + phytohormones mixture, and subjected to high temperatures.
  • the seeds of the corn varieties (Zea mays L.) P70 and Canelo were inoculated with a probiotic mixture containing: the strains ARS BS-19 (Bacillus subtilis), ARS AB-19 (Azospirillum brasilense) and ARS TH-19. (Trichoderma harzianum), RVP MA-20 (Metarhizium anisopliae) and RVP BB-20 (Beauveria bassiana) + a mixture of phytohormones containing: indoleacetic acid and gibberellic acid.
  • the seeds were germinated in germination rolls at 27°C for 96 h; The same was done with the seeds that were stored for 3 months.
  • ARS BS-19 Bacillus subtilis
  • ARS AB-19 Azospirillum brasilense
  • RVP MA-20 Metalarhizium anisopliae
  • RVP BB-20 Berveria bassiana plus indoleacetic acid and gibberellic acid.
  • variable that was quantified was the germination percentage, which averaged the percentage produced in the first and third months.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a mixture comprising: an isolated strain of Trichoderma harzianum (ARS TH-19); an isolated strain of Bacillus subtilis (ARS BS-19); an isolated strain of Azospirillum brasilense (ARS AB-19); an isolated strain of Metarhizium anisopliae (RVP MA-20); an isolated strain of Beauveria bassiana (RVP BB-20); and a mixture of indoleacetic acid and gibberellic acid. The invention also relates to an agricultural product for protecting a plant embryo against extreme temperature and humidity conditions, which comprises the mixture already disclosed. The invention also relates to a method for protecting a plant embryo against extreme temperature and humidity conditions, comprising applying an amount of the agricultural product to plant seeds for sowing.

Description

MEZCLA, PRODUCTO AGRÍCOLA Y MÉTODO, PARA PROTEGER EMBRIONESMIXTURE, AGRICULTURAL PRODUCT AND METHOD, TO PROTECT EMBRYOS
VEGETALES EN CONDICIONES CLIMÁTICAS EXTREMAS VEGETABLES IN EXTREME CLIMATE CONDITIONS
CAMPO TÉCNICO DE LA INVENCIÓN TECHNICAL FIELD OF THE INVENTION
La presente invención se relaciona con el campo técnico de la Biotecnología y Agrícola, ya que aporta una mezcla, un producto agrícola y un método para para proteger embriones de semillas vegetalesdestinadas a la siembra, en contra de condiciones extremas de temperatura y humedad . The present invention is related to the technical field of Biotechnology and Agriculture, since it provides a mixture, an agricultural product and a method to protect embryos of plant seeds intended for sowing, against extreme conditions of temperature and humidity.
ANTECEDENTES DE LA INVENCIÓN BACKGROUND OF THE INVENTION
El tratar semillas vegetales para la siembra de cultivos con plaguicidas inadecuados o en dosis altas , pueden afectar el porcentaj e de germinación o el tiempo de germinación- emergencia . Las especies y variedades de semillas vegetales di fieren en susceptibilidad; por ej emplo , las leguminosas son más susceptibles que los cereales y entre los cereales , el maí z y el trigo son más susceptibles que la cebada ( Castillo-Tovar 2015 ) . Treating vegetable seeds for planting crops with inappropriate pesticides or at high doses can affect the germination percentage or germination-emergence time. Plant seed species and varieties differ in susceptibility; For example, legumes are more susceptible than cereals and among cereals, maize and wheat are more susceptible than barley (Castillo-Tovar 2015).
Es por ello , el interés de obtener productos alternativos para el tratamiento de semillas que serán utili zadas en la siembra de cultivos en los siguientes documentos de patentes se puede observar los intentos de obtener productos elaborados con productos químicos , orgánicos , biológicos , y su combinación entre ellos . For this reason, the interest in obtaining alternative products for the treatment of seeds that will be used in sowing crops in the following patent documents can be seen the attempts to obtain products made with chemical, organic, biological products, and their combination. among them .
El documento de patente EP2019190758A divulga combinaciones de principios activos , en particular una composición fungicida, que comprenden : (A) f luoxapiprolina y (B ) al menos , un agente de control biológico y/o ( C ) al menos , un compuesto seleccionado entre insecticidas, acaricidas y nematicidas. Las composiciones son útiles para controlar especialmente microorganismos dañinos, en particular oomicetos, en la protección de cultivos, para el control de plagas animales y como reguladores del crecimiento de las plantas. Entre los agentes de control biológico, estos pueden ser seleccionados de una gran cantidad de microorganismos, tales como agentes antibacterianos y agentes de control biológico que tienen un efecto para mejorar el crecimiento y/o salud de las plantas; y estos pueden ser del género Bacillus spp . , Pseudomonas spp . , Rizobium spp . , entre otros. Dichos agentes de control biológico ya se encuentran en el estado de la técnica y fueron aislados por terceros. Si bien es cierto que se propone la combinación de agentes de control biológico con un ingrediente químico y opcionalmente un agente fungicida; pero no propone el uso de las cepas microbianas que propone la presente invención, ni la mezcla propuesta . Patent document EP2019190758A discloses combinations of active ingredients, in particular a fungicidal composition, which comprise: (A) fluoxapiproline and (B) at least one biological control agent and/or (C) at least one compound selected from insecticides, acaricides and nematicides. The compositions are useful for controlling especially harmful microorganisms, in particular oomycetes, in crop protection, for the control of animal pests and as plant growth regulators. Among the biological control agents, these can be selected from a large number of microorganisms, such as antibacterial agents and biological control agents that have an effect to improve the growth and/or health of plants; and these may be of the genus Bacillus spp. , Pseudomonas spp. , Rhizobium spp. , among others. These biological control agents are already in the state of the art and were isolated by third parties. Although it is true that the combination of biological control agents with a chemical ingredient and optionally a fungicide agent is proposed; but it does not propose the use of the microbial strains proposed by the present invention, nor the proposed mixture.
El documento de patente WO2014095994A1 divulga composiciones que comprenden un compuesto de trizol; un componente de inhibidores de respiración o inhibidores de los grupos II, III; inhibidores de sintesis de ácidos nucleicos; inhibidores de la división celular y citoesqueleto ; etc.; y una gran gama de bioplaguicidas microbianos, sin especificar cepas de los mismos. Como se puede notar, en este documento se divulgan composiciones muy complejas, donde la mayoría de sus componentes son químicos. Patent document WO2014095994A1 discloses compositions comprising a trizole compound; a component of respiration inhibitors or inhibitors of groups II, III; nucleic acid synthesis inhibitors; inhibitors of cell division and cytoskeleton; etc.; and a wide range of microbial biopesticides, without specifying their strains. As can be noted, this document discloses very complex compositions, where the majority of its components are chemical.
El documento de patente WO2015IB52771A se refiere a la combinación de nuevos inhibidores de la nitrif icación de fórmula I y bioplaguicidas, asi como a la combinación de triamidas de ácido (thio) fosfórico y bioplaguicidas. Además, se refiere al uso de esta combinación de nuevos inhibidores de la nitrif icación y bioplaguicidas para mejorar la salud de una planta, y al uso de la combinación de triamida (s) de ácido (tio) fosfórico y bioplaguicidas para mejorar la salud de una planta; asi como composiciones que comprenden el inhibidor de nitrif icación o (T) y un biopesticida, y composiciones que comprenden la(s) triamida (s) del ácido (tio) fosfórico y un biopesticida. Además, en este documento se divulgan métodos para aumentar la salud de una planta que comprenden el tratamiento de plantas, suelo y/o loci con dicha combinación del nuevo inhibidor de nitrif icación o (T) y un biopesticida o con dicha combinación de (thio) fosfórico. Las composiciones de este documento de patente son muy complejas, donde la mayoría de sus componentes son químicos . The patent document WO2015IB52771A refers to the combination of new nitrification inhibitors of formula I and biopesticides, as well as the combination of (thio) phosphoric acid triamides and biopesticides. Besides, refers to the use of this combination of new nitrification inhibitors and biopesticides to improve the health of a plant, and to the use of the combination of (thio)phosphoric acid triamide(s) and biopesticides to improve the health of a plant ; as well as compositions comprising the nitrification inhibitor or (T) and a biopesticide, and compositions comprising the triamide(s) of (thio) phosphoric acid and a biopesticide. Furthermore, disclosed herein are methods for increasing the health of a plant that comprise treating plants, soil and/or loci with said combination of the new nitrification inhibitor or (T) and a biopesticide or with said combination of (thio ) phosphoric. The compositions of this patent document are very complex, where most of their components are chemical.
Se puede resumir que ninguna de las composiciones o productos divulgados en los documentos antes citados, tienen la finalidad de proteger a los embriones vegetales en condiciones de altas temperaturas y humedad. It can be summarized that none of the compositions or products disclosed in the aforementioned documents have the purpose of protecting plant embryos in conditions of high temperatures and humidity.
Por lo tanto, con la finalidad de contribuir a solucionar los inconvenientes comprendidos en el estado de la técnica, se ha desarrollado una mezcla y un producto agrícola y un método para la protección del embrión de semillas vegetales para siempra, en contra de condiciones adversas de temperatura y humedad. Therefore, in order to contribute to solving the drawbacks included in the state of the art, a mixture and an agricultural product and a method for protecting the embryo of plant seeds forever have been developed, against adverse conditions of temperature and humidity.
Los detalles característicos de la presente invención, se muestran claramente en la siguiente descripción detallada, ejemplo y figura que se acompañan meramente con la finalidad de ilustrar la concepción y algunas realizaciones preferentes de la invención. BREVE DESCRIPCIÓN DE LA FIGURA The characteristic details of the present invention are clearly shown in the following detailed description, example and figure that are accompanied merely for the purpose of illustrating the conception and some preferred embodiments of the invention. BRIEF DESCRIPTION OF THE FIGURE
La figura 1 es una gráfica que ilustra el porcentaje de germinación de semillas de dos variados de maiz, tratadas con la mezcla probiótica + f itohormonas , de la presente invención; y que fueron sometidas altas temperatura . Figure 1 is a graph that illustrates the percentage of germination of seeds of two varieties of corn, treated with the probiotic + phytohormones mixture of the present invention; and that they were subjected to high temperatures.
DESCRIPCIÓN DE LA INVENCIÓN DESCRIPTION OF THE INVENTION
La presente invención, tiene como primer objeto a una mezcla, que contiene: i) una cepa aislada de Trichoderma harzianum, la cual es la cepa ARS TH-19, depositada con el número de depósito CM-CNRG TB174; ii) una cepa aislada de Bacillus subtilis la cual es la cepa ARS BS-19, depositada con el número de depósito CM-CNRG TB175; iii) una cepa aislada de Azospirillum brasilense, la cual es la cepa ARS AB-19, depositada con el número de depósito CM-CNRG TB176; iv) una cepa aislada de Metarhizium anisopliae; la cual es la cepa RVP MA-20, depositada con el número de depósito CM-CNRG TB195; v) una cepa aislada de Beauveria bassiana, la cual es la cepa RVP BB-20, depositada con el número de depósito CM-CNRG TB196; y vi) una mezcla de ácido indolacético y ácido giberélico, la cual se observó que tiene efectos positivos para la protección del embrión en condiciones extremas de temperatura y humedad. Una modalidad de la mezcla de la presente invención, es cuando la suma de las cantidades de las cepas aislada es de un 37 % y la mezcla de ácido indolacético y ácido giberélico está en una cantidad de un 0 . 006% . The first object of the present invention is a mixture that contains: i) an isolated strain of Trichoderma harzianum, which is the ARS TH-19 strain, deposited with the deposit number CM-CNRG TB174; ii) an isolated strain of Bacillus subtilis which is the ARS BS-19 strain, deposited with the deposit number CM-CNRG TB175; iii) an isolated strain of Azospirillum brasilense, which is the ARS AB-19 strain, deposited with the deposit number CM-CNRG TB176; iv) an isolated strain of Metarhizium anisopliae; which is the RVP MA-20 strain, deposited with the deposit number CM-CNRG TB195; v) an isolated strain of Beauveria bassiana, which is the RVP BB-20 strain, deposited with the deposit number CM-CNRG TB196; and vi) a mixture of indoleacetic acid and gibberellic acid, which was observed to have positive effects for the protection of the embryo in extreme conditions of temperature and humidity. One modality of the mixture of the present invention is when the sum of the amounts of the isolated strains is 37% and the mixture of indoleacetic acid and gibberellic acid is in an amount of 0. 006% .
Un segundo obj eto de la presente invención es un producto agrícola para proteger a un embrión vegetal en contra de condiciones extremas de temperaturas y humedad, donde dicho producto agrícola comprende , a la mezcla de conformidad con la presente invención . A second object of the present invention is an agricultural product for protecting a plant embryo against extreme conditions of temperatures and humidity, where said agricultural product comprises the mixture in accordance with the present invention.
Donde una reali zación del producto agrícola en cuestión es cuando la protección es para un embrión vegetal de plantas de la familia Poaceae . Where a realization of the agricultural product in question is when the protection is for a plant embryo of plants of the Poaceae family.
Un tercer obj eto de esta invención es un método para proteger a un embrión vegetal de las condiciones extremas de temperatura y humedad, el cual comprende , aplicar una cantidad efectiva del producto agrícola de conformidad con la presente invención, a semillas vegetales destinadas a la siembra . A third object of this invention is a method for protecting a plant embryo from extreme conditions of temperature and humidity, which comprises applying an effective amount of the agricultural product in accordance with the present invention, to plant seeds intended for sowing. .
Donde una reali zación del Método de la presente invención es cuando la cantidad efectiva del producto agrícola es de 1 mL por 1 Kg de semillas . Where one embodiment of the Method of the present invention is when the effective amount of the agricultural product is 1 mL per 1 Kg of seeds.
Una reali zación preferente de dicho método , es cuando la protección es para un embrión de plantas de la familia Poaceae . EJEMPLOS DE LA INVENCIÓN A preferred embodiment of said method is when the protection is for an embryo of plants of the Poaceae family. EXAMPLES OF THE INVENTION
Los siguientes ejemplos son para ilustrar la concepción de la presente invención y algunas de sus realizaciones preferentes. Por lo tanto, tales ejemplos no deben ser considerados como una limitante para el alcance de protección de dicha invención. The following examples are to illustrate the conception of the present invention and some of its preferred embodiments. Therefore, such examples should not be considered as a limitation for the scope of protection of said invention.
Ejemplo 1. Evaluar la conservación de la capacidad germinativa del embrión de semillas de maiz, después de ser tratadas con una mezcla probiótica + f itohormonas , y sometidas a altas temperaturas. Example 1. Evaluate the conservation of the germination capacity of the embryo of corn seeds, after being treated with a probiotic + phytohormones mixture, and subjected to high temperatures.
Ob j etivo Aim
Evaluar la conservación de la capacidad germinativa del embrión de semillas de malees de las variedades P70 y Canelo, después de ser tratadas con la mezcla probiótica + una mezcla de f itohormonas , y sometidas a altas temperaturas. To evaluate the conservation of the germination capacity of the embryo of male seeds of the P70 and Canelo varieties, after being treated with the probiotic mixture + a mixture of phytohormones, and subjected to high temperatures.
Metodología Methodology
Las semillas de las variedades de maíz (Zea mays L.) P70 y Canelo, fueron inoculadas con una mezcla probiótica que contiene: las cepas ARS BS-19 (Bacillus subtilis) , ARS AB- 19 (Azospirillum brasilense) y ARS TH-19 (Trichoderma harzianum) , RVP MA-20 (Metarhizium anisopliae) y RVP BB-20 (Beauveria bassiana) + una mezcla de fitohormonas que contiene: ácido indolacético y ácido giberelico. The seeds of the corn varieties (Zea mays L.) P70 and Canelo were inoculated with a probiotic mixture containing: the strains ARS BS-19 (Bacillus subtilis), ARS AB-19 (Azospirillum brasilense) and ARS TH-19. (Trichoderma harzianum), RVP MA-20 (Metarhizium anisopliae) and RVP BB-20 (Beauveria bassiana) + a mixture of phytohormones containing: indoleacetic acid and gibberellic acid.
Una vez inoculadas las semillas fueron sometidas a un envejecimiento acelerado en condiciones de 42 °C por 96 h y se almacenaron durante 1 y 3 meses. -1-Once inoculated, the seeds were subjected to accelerated aging in conditions of 42 °C for 96 h and were stored for 1 and 3 months. -1-
Una vez cumplido el primer mes, las semillas fueron puestas a germinar en rollos de germinación a 27°C por 96 h; lo mismo se hizo con las semillas que se almacenaron durante 3 meses. Once the first month was over, the seeds were germinated in germination rolls at 27°C for 96 h; The same was done with the seeds that were stored for 3 months.
Por lo que los tratamientos fueron: So the treatments were:
- Semilla testigo, sin inoculación con la mezcla. - Control seed, without inoculation with the mixture.
- Semillas inoculadasARS BS-19 (Bacillus subtilis) , ARS AB-19 (Azospirillum brasilense) y ARS TH-19- Inoculated seeds ARS BS-19 (Bacillus subtilis), ARS AB-19 (Azospirillum brasilense) and ARS TH-19
(Trichoderma harzianum) , RVP MA-20 (Metarhizium anisopliae) y RVP BB-20 (Beauveria bassiana) más acido indolacético y ácido giberélico. (Trichoderma harzianum), RVP MA-20 (Metarhizium anisopliae) and RVP BB-20 (Beauveria bassiana) plus indoleacetic acid and gibberellic acid.
- Maxim Quattro. -Maxim Quattro.
- Oxys SST. - Oxys SST.
- Damp SST - Damp SST
La variable que se cuantificó fue el porcentaje de germinación, la cual se promedió el porcentaje arrojado en primer y tercer mes. The variable that was quantified was the germination percentage, which averaged the percentage produced in the first and third months.
Resultados Results
En la figura 1 se puede ver que la variedad Canelo siempre presentó los más altos porcentajes de germinación, en comparación con la variedad P70; y que las semillas de ambas variedades que fueron tratadas con la mezcla probiótica + la mezcla de f itohormonas , fueron las que mayor porcentaje de germinación presentaron, en comparación con los otros tratamientos . In figure 1 it can be seen that the Canelo variety always presented the highest germination percentages, compared to the P70 variety; and that the seeds of both varieties that were treated with the probiotic mixture + the phytohormone mixture were the ones with the highest germination percentage, compared to the other treatments.
Por lo tanto, se puede concluir que la mezcla probiótica + mezcla de hormonas, si protegen a los embriones vegetales cuando se exponen a condiciones adversas de temperatura. LITERATURA CITADA Therefore, it can be concluded that the probiotic mixture + hormone mixture does protect plant embryos when exposed to adverse temperature conditions. LITERATURE CITED
Castillo-Tovar, H. 2015. El tratamiento de semilla para la siembra de maiz en P-V. Boletín electrónico Año 1, No. 14. Campo Experimental, Centro de Investigación Regional del Noreste, INIFAP. Páginas 2. Castillo-Tovar, H. 2015. Seed treatment for corn sowing in P-V. Electronic Bulletin Year 1, No. 14. Experimental Field, Northeast Regional Research Center, INIFAP. Pages 2.

Claims

REIVINDICACIONES
1. Una mezcla, caracterizada porque comprende: i) una cepa aislada de Trichoderma harzianum, la cual es la cepa ARS TH-19, depositada con el número de depósito CM-CNRG TB174; ii) una cepa aislada de Bacillus subtilis la cual es la cepa ARS BS-19, depositada con el número de depósito CM-CNRG TB175; iii) una cepa aislada de Azospirillum brasilense, la cual es la cepa ARS AB-19, depositada con el número de depósito CM-CNRG TB176; iv) una cepa aislada de Metarhizium anisopliae; la cual es la cepa RVP MA-20, depositada con el número de depósito CM-CNRG TB195; v) una cepa aislada de Beauveria bassiana, la cual es la cepa RVP BB-20, depositada con el número de depósito CM-CNRG TB196; y vi) una mezcla de ácido indolacético y ácido giberélico . 1. A mixture, characterized in that it comprises: i) an isolated strain of Trichoderma harzianum, which is the ARS TH-19 strain, deposited with the deposit number CM-CNRG TB174; ii) an isolated strain of Bacillus subtilis which is the ARS BS-19 strain, deposited with the deposit number CM-CNRG TB175; iii) an isolated strain of Azospirillum brasilense, which is the ARS AB-19 strain, deposited with the deposit number CM-CNRG TB176; iv) an isolated strain of Metarhizium anisopliae; which is the RVP MA-20 strain, deposited with the deposit number CM-CNRG TB195; v) an isolated strain of Beauveria bassiana, which is the RVP BB-20 strain, deposited with the deposit number CM-CNRG TB196; and vi) a mixture of indoleacetic acid and gibberellic acid.
2. La mezcla de la reivindicación anterior, donde la suma de la cantidad de las cepas aisladas es de un 37%. 2. The mixture of the previous claim, where the sum of the amount of the isolated strains is 37%.
3. La mezcla de la reivindicación 1, donde la mezcla de ácido indolacético y ácido giberélico está en una cantidad de un 0.006%. 3. The mixture of claim 1, wherein the mixture of indoleacetic acid and gibberellic acid is in an amount of 0.006%.
4. Un producto agrícola para proteger a un embrión vegetal en contra de condiciones extremas de temperaturas y humedad, caracterizado porque comprende, a la mezcla de conformidad con las reivindicaciones anteriores. 4. An agricultural product to protect a plant embryo against extreme conditions of temperatures and humidity, characterized in that it comprises the mixture according to the preceding claims.
5 . El producto agrícola de la reivindicación anterior, donde el embrión vegetal es de una planta de la familia Poaceae . 5 . The agricultural product of the previous claim, where the plant embryo is from a plant of the Poaceae family.
6 . Un método para proteger a un embrión vegetal de las condiciones extremas de temperatura y humedad, caracteri zado porque comprende , aplicar una cantidad efectiva del producto agrícola de conformidad con las reivindicaciones 3 y 4 , a semillas vegetales destinadas a la siembra . 6. A method for protecting a plant embryo from extreme conditions of temperature and humidity, characterized in that it comprises applying an effective amount of the agricultural product in accordance with claims 3 and 4, to plant seeds intended for sowing.
7 . El Método de la reivindicación, donde la cantidad efectiva del producto agrícola es de 1 mL por 1 Kg de semillas . 7. The Claim Method, where the effective amount of the agricultural product is 1 mL per 1 Kg of seeds.
8 . El método de la reivindicación 5 , donde el embrión vegetal pertenece a semillas de plantas de la familia8. The method of claim 5, wherein the plant embryo belongs to seeds of plants of the family
Poaceae . Poaceae.
PCT/MX2023/050018 2022-05-09 2023-03-01 Mixture, agricultural product and method to protect plant embryos under extreme climate conditions WO2023219485A1 (en)

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