WO2023219485A1 - Mélange, produit agricole et procédé pour protéger des embryons végétaux dans des conditions climatiques extrêmes - Google Patents

Mélange, produit agricole et procédé pour protéger des embryons végétaux dans des conditions climatiques extrêmes Download PDF

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Publication number
WO2023219485A1
WO2023219485A1 PCT/MX2023/050018 MX2023050018W WO2023219485A1 WO 2023219485 A1 WO2023219485 A1 WO 2023219485A1 MX 2023050018 W MX2023050018 W MX 2023050018W WO 2023219485 A1 WO2023219485 A1 WO 2023219485A1
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WO
WIPO (PCT)
Prior art keywords
mixture
strain
agricultural product
ars
plant
Prior art date
Application number
PCT/MX2023/050018
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English (en)
Spanish (es)
Inventor
Luis Rodrigo BECERRA CARRANZA
Original Assignee
Becerra Carranza Luis Rodrigo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Becerra Carranza Luis Rodrigo filed Critical Becerra Carranza Luis Rodrigo
Publication of WO2023219485A1 publication Critical patent/WO2023219485A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma

Definitions

  • the present invention is related to the technical field of Biotechnology and Agriculture, since it provides a mixture, an agricultural product and a method to protect embryos of plant seeds intended for sowing, against extreme conditions of temperature and humidity.
  • Treating vegetable seeds for planting crops with inappropriate pesticides or at high doses can affect the germination percentage or germination-emergence time.
  • Plant seed species and varieties differ in susceptibility; For example, legumes are more susceptible than cereals and among cereals, maize and wheat are more susceptible than barley (Castillo-Tovar 2015).
  • Patent document EP2019190758A discloses combinations of active ingredients, in particular a fungicidal composition, which comprise: (A) fluoxapiproline and (B) at least one biological control agent and/or (C) at least one compound selected from insecticides, acaricides and nematicides.
  • the compositions are useful for controlling especially harmful microorganisms, in particular oomycetes, in crop protection, for the control of animal pests and as plant growth regulators.
  • the biological control agents these can be selected from a large number of microorganisms, such as antibacterial agents and biological control agents that have an effect to improve the growth and/or health of plants; and these may be of the genus Bacillus spp.
  • Patent document WO2014095994A1 discloses compositions comprising a trizole compound; a component of respiration inhibitors or inhibitors of groups II, III; nucleic acid synthesis inhibitors; inhibitors of cell division and cytoskeleton; etc.; and a wide range of microbial biopesticides, without specifying their strains. As can be noted, this document discloses very complex compositions, where the majority of its components are chemical.
  • the patent document WO2015IB52771A refers to the combination of new nitrification inhibitors of formula I and biopesticides, as well as the combination of (thio) phosphoric acid triamides and biopesticides. Besides, refers to the use of this combination of new nitrification inhibitors and biopesticides to improve the health of a plant, and to the use of the combination of (thio)phosphoric acid triamide(s) and biopesticides to improve the health of a plant ; as well as compositions comprising the nitrification inhibitor or (T) and a biopesticide, and compositions comprising the triamide(s) of (thio) phosphoric acid and a biopesticide.
  • compositions of this patent document are very complex, where most of their components are chemical.
  • compositions or products disclosed in the aforementioned documents have the purpose of protecting plant embryos in conditions of high temperatures and humidity.
  • Figure 1 is a graph that illustrates the percentage of germination of seeds of two varieties of corn, treated with the probiotic + phytohormones mixture of the present invention; and that they were subjected to high temperatures.
  • the first object of the present invention is a mixture that contains: i) an isolated strain of Trichoderma harzianum, which is the ARS TH-19 strain, deposited with the deposit number CM-CNRG TB174; ii) an isolated strain of Bacillus subtilis which is the ARS BS-19 strain, deposited with the deposit number CM-CNRG TB175; iii) an isolated strain of Azospirillum brasilense, which is the ARS AB-19 strain, deposited with the deposit number CM-CNRG TB176; iv) an isolated strain of Metarhizium anisopliae; which is the RVP MA-20 strain, deposited with the deposit number CM-CNRG TB195; v) an isolated strain of Beauveria bassiana, which is the RVP BB-20 strain, deposited with the deposit number CM-CNRG TB196; and vi) a mixture of indoleacetic acid and gibberellic acid, which was observed to have positive effects for the protection of the
  • a second object of the present invention is an agricultural product for protecting a plant embryo against extreme conditions of temperatures and humidity, where said agricultural product comprises the mixture in accordance with the present invention.
  • a third object of this invention is a method for protecting a plant embryo from extreme conditions of temperature and humidity, which comprises applying an effective amount of the agricultural product in accordance with the present invention, to plant seeds intended for sowing. .
  • Method of the present invention is when the effective amount of the agricultural product is 1 mL per 1 Kg of seeds.
  • a preferred embodiment of said method is when the protection is for an embryo of plants of the Poaceae family.
  • Example 1 Evaluate the conservation of the germination capacity of the embryo of corn seeds, after being treated with a probiotic + phytohormones mixture, and subjected to high temperatures.
  • the seeds of the corn varieties (Zea mays L.) P70 and Canelo were inoculated with a probiotic mixture containing: the strains ARS BS-19 (Bacillus subtilis), ARS AB-19 (Azospirillum brasilense) and ARS TH-19. (Trichoderma harzianum), RVP MA-20 (Metarhizium anisopliae) and RVP BB-20 (Beauveria bassiana) + a mixture of phytohormones containing: indoleacetic acid and gibberellic acid.
  • the seeds were germinated in germination rolls at 27°C for 96 h; The same was done with the seeds that were stored for 3 months.
  • ARS BS-19 Bacillus subtilis
  • ARS AB-19 Azospirillum brasilense
  • RVP MA-20 Metalarhizium anisopliae
  • RVP BB-20 Berveria bassiana plus indoleacetic acid and gibberellic acid.
  • variable that was quantified was the germination percentage, which averaged the percentage produced in the first and third months.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Selon l'invention, un mélange comprend une souche isolée de Trichoderma harzianum (ARS TH-19); une souche isolée de Bacillus subtilis (ARS BS-19); une souche isolée d'Azospirillum brasilense (ARS AB-19); une souche isolée de Metarhizium anisopliae (RVP MA-20); une souche isolée de Beauveria bassiana (RVP BB-20); et un mélange d'acide indolacétique et d'acide gibbérellique. Selon l'invention, un produit agricole pour la protection d'un embryon végétal face à des conditions extrêmes de température et d'humidité comprend le mélange décrit précédemment. L'invention concerne aussi un procédé pour la protection d'un embryon végétal face à des conditions extrêmes de température et d'humidité, lequel procédé consiste à appliquer une quantité du produit agricole sur des semences végétales pour l'ensemencement.
PCT/MX2023/050018 2022-05-09 2023-03-01 Mélange, produit agricole et procédé pour protéger des embryons végétaux dans des conditions climatiques extrêmes WO2023219485A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MXMX/A/2022/005607 2022-05-09
MX2022005607 2022-05-09

Publications (1)

Publication Number Publication Date
WO2023219485A1 true WO2023219485A1 (fr) 2023-11-16

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PCT/MX2023/050018 WO2023219485A1 (fr) 2022-05-09 2023-03-01 Mélange, produit agricole et procédé pour protéger des embryons végétaux dans des conditions climatiques extrêmes

Country Status (1)

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WO (1) WO2023219485A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003020038A1 (fr) * 2001-09-04 2003-03-13 Council Of Scientific And Industrial Research Composition bioinoculante contenant des souches bacteriennes de b. subtilis ou de b. lentimorbus provenant du lait de vache
WO2014147528A1 (fr) * 2013-03-20 2014-09-25 Basf Corporation Compositions synergiques comprenant une souche de bacillus subtilis et un biopesticide
CN110870484A (zh) * 2019-11-25 2020-03-10 华南农业大学 一种金龟子绿僵菌与呋虫胺的复配杀虫剂及其应用
CN112143658A (zh) * 2020-10-20 2020-12-29 中国热带农业科学院香料饮料研究所 一种球孢白僵菌菌株mq-08及其应用和微生物制剂

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003020038A1 (fr) * 2001-09-04 2003-03-13 Council Of Scientific And Industrial Research Composition bioinoculante contenant des souches bacteriennes de b. subtilis ou de b. lentimorbus provenant du lait de vache
WO2014147528A1 (fr) * 2013-03-20 2014-09-25 Basf Corporation Compositions synergiques comprenant une souche de bacillus subtilis et un biopesticide
CN110870484A (zh) * 2019-11-25 2020-03-10 华南农业大学 一种金龟子绿僵菌与呋虫胺的复配杀虫剂及其应用
CN112143658A (zh) * 2020-10-20 2020-12-29 中国热带农业科学院香料饮料研究所 一种球孢白僵菌菌株mq-08及其应用和微生物制剂

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A BD EL-DAIM ISLAM A ET AL.: "Improved heat stress tolerance of wheat seedlings by bacterial seed treatment", PLANT AND SOIL, vol. 379, no. 1-2, 2014, pages 337 - 350, XP035318411, ISSN: 0032-079X, DOI: 10.1007/s11104-014-2063-3 *
MASTOURI FATEMEH ET AL.: "Seed Treatment with Trichoderma harzianum Alleviates Biotic, Abiotic,and Physiological Stresses in Germinating Seeds and Seedlings", PHYTOPATHOLOGY, vol. 100, no. 11, 2010, pages 1213 - 1221, XP055258979, ISSN: 0031- 949X, DOI: 10.1094/PHYTO-03-10-0091 *
SULTANA NILUFA, IKEDA TAKESHI, MITSUI TOSHIAKI: "GA 3 and Proline Promote Germination of Wheat Seeds by Stimulating α -Amylase at Unfavorable Temperatures", PLANT PRODUCTION SCIENCE, CROP SCIENCE SOCIETY OF JAPAN, TOKYO, JP, vol. 3, no. 3, 1 January 2000 (2000-01-01), JP , pages 232 - 237, XP093110417, ISSN: 1343-943X, DOI: 10.1626/pps.3.232 *

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