WO2023219484A1 - Mélanges probiotiques, bioproduits agricoles et procédés pour améliorer la capacité de germination des semences végétales d'ensemencement - Google Patents

Mélanges probiotiques, bioproduits agricoles et procédés pour améliorer la capacité de germination des semences végétales d'ensemencement Download PDF

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Publication number
WO2023219484A1
WO2023219484A1 PCT/MX2023/050017 MX2023050017W WO2023219484A1 WO 2023219484 A1 WO2023219484 A1 WO 2023219484A1 MX 2023050017 W MX2023050017 W MX 2023050017W WO 2023219484 A1 WO2023219484 A1 WO 2023219484A1
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WIPO (PCT)
Prior art keywords
strain
seeds
bioproduct
cnrg
agricultural
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PCT/MX2023/050017
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English (en)
Spanish (es)
Inventor
Luis Rodrigo BECERRA CARRANZA
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Becerra Carranza Luis Rodrigo
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Publication of WO2023219484A1 publication Critical patent/WO2023219484A1/fr

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22Bacillus
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/32Yeast
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38Trichoderma

Definitions

  • the present invention is related to the technical field of Biotechnology and Agriculture, since it provides probiotic mixtures, agricultural bioproducts and methods to improve the germination capacity of vegetable seeds, preferably those intended for sowing.
  • Treating vegetable seeds for planting crops with inappropriate pesticides or at high doses can affect the germination percentage or germination-emergence time.
  • Plant seed species and varieties differ in susceptibility; For example, legumes are more susceptible than cereals and among cereals, corn and wheat are more susceptible than barley (Castillo-Tovar 2015).
  • the patent document MX/a/2017/014720 refers to a foliar application microbial consortium that acts as a biofertilizer and as a biological control agent. Between Its main functions are the stimulation of plant growth, the reduction of stress in the face of environmental fluctuations and the biological control of phytopathogens.
  • the consortium includes the following genera of bacteria and fungi: Bacillus, Pseudomonas, Streptomyces, Azotobacter, Azospirillum, Beijerinckia, Trichoderma and Aspergillus, within which the species Bacillus subtilis, Bacillus azoto formas are considered; Bacillus megaterium, Bacillus pumilus, Bacillus polymyxa, Bacillus sphaericus, Bacillus stearothermophilus; Pseudomonas fluorescens, Pseudomonas putida, Streptomyces avermitilis; Streptomyces noursei; Streptomyces griseus, Azotobacter chroococcum, Azotobacter vinelandii, Azospirillum brasilense; Azospirillum oryzae, Beijerinckia indica; Beijerinckia derxir, Trichoderma viride,
  • the species of the consortium can be epiphytic or endophytic and act in the aerial structures of plants. It includes nitrogen-fixing bacteria (BFN) that guarantee that nitrogen is fixed near the plant's assimilation sites, which is why they participate in the incorporation of nitrogen into organic matter.
  • BFN nitrogen-fixing bacteria
  • this document proposes the use of bacterial and fungal microorganisms, said consortium involves a large number of strains, and it is not evident that said consortium has an industrial application, since the strains involved are not specified. Nor is the existence of said strains evident, for example, if they are deposited in a recognized patent office, etc.
  • the fertilizer is made from, by weight, 5-10 parts of composite microorganisms, 10-30 parts of botanical organic matter, 5-20 parts of organic matter of animal origin, 3-10 parts of medium trace elements and 2-10 parts of other active additives.
  • the fertilizer is made from, by weight, 5-10 parts of composite microorganisms, 10-30 parts of botanical organic matter, 5-20 parts of organic matter of animal origin, 3-10 parts of medium trace elements and 2-10 parts of other active additives.
  • the solids obtained after composting are combined with the medium trace elements and other active substances to obtain a product.
  • the special growth-promoting and disease-resistant bacterial fertilizer has the advantages that great growth-promoting function and resistance effectiveness to diseases and insect damage are achieved, and after the application of the fertilizer, the rate of occurrence of diseases and insect damage of Radix astragali is obviously lower than that of common compound fertilizers, while the yield and quality of R. astragali mixture are obviously higher than those of common compound fertilizers.
  • microorganisms are the probiotic strains: Bacillus subtilis, Bacillus cereus, Bacillus polymyxa, Trichoderma harzianum; N-fixing strains: Azorhizobium tuberculosis, Azospirillum brasiliensis; growth promoting strains: Bacillus polymyxa, Streptomyces marine, Streptomyces avermitilis; and strains with an insecticidal effect: Beauveria bassiana, Beauveria bruce!, Metarhizium anisopliae.
  • the patent document EP2019190758A discloses combinations of active ingredients, in particular a fungicidal composition, which comprise: (A) fluoxapiproline and (B) at least one biological control agent and/or (C) at least one compound selected from insecticides, acaricides and nematicides.
  • the compositions are useful for controlling especially harmful microorganisms, in particular oomycetes, in crop protection, for the control of animal pests and as plant growth regulators.
  • biological control agents these can be selected from a large number of microorganisms, such as antibacterial agents and biological control agents that have an effect to improve the growth and/or health of plants; and these may be of the genus Bacillus spp. , Pseudomonas spp. , Rhizobium spp. , among others.
  • These biological control agents are already in the state of the art and were isolated by third parties. It can be concluded from this document that it is the combination of biological control agents with a chemical ingredient and optionally a fungicide agent.
  • strains of Bacillus spp., Pseudomonas spp., Rizobium spp. that are proposed are isolated in geographical regions outside our country or similar regions.
  • the patent document WO2018IB54949A describes mixtures that comprise a biopesticide and an inhibitor of nitri fication.
  • a large number of genera and species of microorganisms are listed among biopesticides; and also a wide range of nitrification inhibitors, among which biological and synthetic chemical nitrification inhibitor groups are mentioned.
  • the use of such mixtures is to increase plant health and/or provide better crop yields and/or better quality of plants or crops, and/or show better resistance to stress, and/or allow reducing the amount of pesticides used, and/or helping to avoid the development of resistance to said pesticides.
  • probiotic mixtures, agricultural bioproducts and methods have been developed to improve the germination capacity of vegetable seeds, preferably those intended for sowing.
  • a first object of the present invention is a probiotic mixture 1, which comprises: i) an isolated strain of Trichoderma harzianum, which is the ARS TH-19 strain deposited with the deposit number CM-CNRG TB174; ii) an isolated strain of Bacillus subtilis which is the ARS BS-19 strain deposited with the deposit number CM-CNRG TB175; and iii) an isolated strain of Azospirillum brasilense, which is the ARS AB-19 strain, deposited with the deposit number CM-CNRG TB176.
  • probiotic mixture of the present invention is when the strains are in a 1:1:1 ratio.
  • a second object of the present invention is a probiotic mixture 2, which is the same probiotic mixture 1, but also comprises: i) an isolated strain of Metarhizium anisopliae (RVP MA-20); which is the RVP MA-20 strain, deposited with the deposit number CM-CNRG TB195; and ii) an isolated strain of Beauveria bassiana, which is the RVP BB-20 strain, deposited with the deposit number CM-CNRG TB196.
  • RVP MA-20 Metarhizium anisopliae
  • a third object of this invention is a probiotic mixture 3, which is the same probiotic mixture 2, but also comprises an isolated strain of Bacillus thuringiensis var israelensis, which is the RVP BTI-20 strain, deposited with the number of tank CM-CNRG TB193. Where said isolated strain is present in an amount of 29.1%.
  • a fourth object of the present invention is an agricultural bioproduct to increase and preserve for a longer time the germination capacity of the embryo of plant seeds, where said bioproduct comprises the probiotic mixture 1 in accordance with this invention.
  • a fifth object of this invention is an agricultural bioproduct to increase the production of effective leaf area in vegetable seedlings, which contains the probiotic mixture 2 described in this invention.
  • One embodiment of this bioproduct is when the plant seeds are from plants of the Poaceae family.
  • a sixth object of the present invention is an agricultural bioproduct to increase the speed of emergence of vegetable seeds, which comprises the probiotic mixture 3 in accordance with the present invention. It is also a preferred embodiment of said agricultural bioproduct, when the plant seeds are from plants of the Poaceae family.
  • a seventh object of the invention in question is a method to increase and conserve for a longer time, the germination capacity of the embryo of plant seeds, which comprises, applying an effective amount of the agricultural bioproduct to increase and conserve for a longer time, the capacity germinative of the embryo of vegetable seeds, to seeds and/or vegetable grains intended for sowing.
  • An embodiment of this method is when the effective amount of the agricultural bioproduct is 1 mL per 1 Kg of seeds and/or grains; and such plant seeds and/or grains are from plants of the Poaceae family.
  • the present invention also has as its eighth object a method to increase the production of effective leaf area in plant seedlings, which comprises applying an effective amount of the bioproduct useful to increase the production of effective leaf area in plant seedlings, to seeds and/or grains. vegetables intended for planting.
  • the effective amount of the agricultural bioproduct is 1 mL per 1 kg of seeds and/or grains; and where the seeds and/or vegetable grains are from plants of the Poaceae family, preferably.
  • This invention also has a ninth object, which is a method to increase the speed of emergence in vegetable seeds, which comprises applying an effective amount of the bioproduct to increase the speed of emergence in vegetable seeds, to seeds and/or vegetable grains destined for planting.
  • the effective amount of the agricultural bioproduct is 1 mL per 1 kg of seeds and/or grains; and the plant seeds and/or grains are from plants of the Poaceae family.

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

Selon l'invention, des mélanges probiotiques comprennent : (A) une souche isolée de Trichoderma harzianum, laquelle est la souche ARS TH-19 déposée en tant que CM-CNRG TB174; une souche isolée de Bacillus subtilis, laquelle est la souche ARS BS-19 déposée en tant que CM-CNRG TB175; et une souche isolée de Azospirillum brasilense, laquelle est la souche ARS AB-19 déposée en tant que CM-CNRG TB176; (B) une souche isolée de Metarhizium anisopliae (RVP MA-20) laquelle est la souche RVP MA-20 déposée en tant que CM-CNRG TB195; et une souche isolée de Beauveria bassiana, laquelle est la souche RVP BB-20 déposée en tant que CM-CNRG TB196; et (C) une souche isolée de Bacillus thuringiensis var israelensis, laquelle est la souche RVP BTI-20 déposée en tant que CM-CNRG TB193. Lesdits bioproduits agricoles comprennent lesdits mélanges probiotiques. L'invention concerne aussi des procédés pour augmenter et conserver plus longtemps la capacité germinative de l'embryon, augmenter la zone foliaire effective et la vitesse d'émergence dans des semences végétales destinées à l'ensemencement.
PCT/MX2023/050017 2022-05-09 2023-03-01 Mélanges probiotiques, bioproduits agricoles et procédés pour améliorer la capacité de germination des semences végétales d'ensemencement WO2023219484A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MX2022005608 2022-05-09
MXMX/A/2022/005608 2022-05-09

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WO2023219484A1 true WO2023219484A1 (fr) 2023-11-16

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010109436A1 (fr) * 2009-03-25 2010-09-30 Carepro Bioscience (P) Ltd Formulation microbienne conçue pour une utilisation étendue en agriculture
WO2014147528A1 (fr) * 2013-03-20 2014-09-25 Basf Corporation Compositions synergiques comprenant une souche de bacillus subtilis et un biopesticide
CN107619335A (zh) * 2017-03-29 2018-01-23 济南航晨生物科技有限公司 一种黄芪专用促生抗病菌肥
MX2017014720A (es) * 2017-11-16 2019-05-17 Jose Alfredo Suarez Rivero Consorcio microbiano para uso agricola y formulacion que lo contiene.
WO2021091365A1 (fr) * 2019-11-06 2021-05-14 Becerra Carranza Luis Rodrigo Mélange et produit organique à usage agricole, et leurs procédés de préparation et d'application
WO2022191693A1 (fr) * 2021-03-12 2022-09-15 Becerra Carranza Luis Rodrigo Mélanges et procédé pour la lutte contre des agents phytopathogènes attaquant les semences végétales pendant leur germination

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010109436A1 (fr) * 2009-03-25 2010-09-30 Carepro Bioscience (P) Ltd Formulation microbienne conçue pour une utilisation étendue en agriculture
WO2014147528A1 (fr) * 2013-03-20 2014-09-25 Basf Corporation Compositions synergiques comprenant une souche de bacillus subtilis et un biopesticide
CN107619335A (zh) * 2017-03-29 2018-01-23 济南航晨生物科技有限公司 一种黄芪专用促生抗病菌肥
MX2017014720A (es) * 2017-11-16 2019-05-17 Jose Alfredo Suarez Rivero Consorcio microbiano para uso agricola y formulacion que lo contiene.
WO2021091365A1 (fr) * 2019-11-06 2021-05-14 Becerra Carranza Luis Rodrigo Mélange et produit organique à usage agricole, et leurs procédés de préparation et d'application
WO2022191693A1 (fr) * 2021-03-12 2022-09-15 Becerra Carranza Luis Rodrigo Mélanges et procédé pour la lutte contre des agents phytopathogènes attaquant les semences végétales pendant leur germination

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