WO2023216514A1 - Traditional chinese medicine extract composition for preventing and treating influenza, preparation method therefor and use thereof - Google Patents

Abstract

A traditional Chinese medicine extract composition for preventing and treating influenza, comprising the following components: an Angelica sinensis extract, a Ligusticum wallichii extract and pharmaceutically acceptable excipients. The Angelica sinensis extract is an Angelica sinensis volatile oil, the Ligusticum wallichii extract is a Ligusticum wallichii volatile oil, and the volatile oils are obtained by means of supercritical or subcritical extraction. The Angelica sinensis extract contains ferulic acid and ligustilide, and the Ligusticum wallichii extract contains ligustilide and senkyunolide A. The traditional Chinese medicine extract composition has a good therapeutic effect on diseases caused by influenza viruses and can significantly prolong the survival of influenza virus mice, reduce the mortality rate among influenza virus mice, significantly reduce the lung index of influenza virus mice, and effectively inhibit lung injury caused by influenza viruses.

Description

A traditional Chinese medicine extract composition for preventing and treating influenza and its preparation method and application

This application requires the priority of the Chinese patent application submitted to the China Patent Office on May 13, 2022, with the application number 202210532703.3 and the invention title "A Chinese medicine extract composition for preventing and treating influenza and its preparation method and application", which The entire contents are incorporated herein by reference.

Technical field

The invention relates to the field of medicine, specifically a traditional Chinese medicine extract composition for preventing and treating influenza and its preparation method and application.

Background technique

Influenza is an acute respiratory infectious disease caused by influenza viruses. It is most prevalent in spring and autumn, especially in the Asia-Pacific region. In high-risk groups such as children, pregnant women, the elderly, and immunodeficiency groups, influenza viruses can cause respiratory tract infections in the lungs, liver, and other organs. Injury, in severe cases, can lead to multiple organ failure, worsening of underlying diseases, or even death.

At present, some western medicines are commonly used to prevent and treat influenza, with symptomatic treatment being the mainstay. Due to the variability of influenza viruses, the efficacy of anti-influenza virus treatments is limited. For example, oseltamivir can convert esterase in the intestine into active metabolites, thereby interfering with the spread of the virus, but it is not suitable for patients who have symptoms of lower respiratory tract infection; peramivir is the only intravenous neuramine Acidase inhibitors are more effective than other neuraminidase inhibitors and can shorten the fever reduction time more effectively than oral oseltamivir. However, they may be mutually resistant to other neuraminidase inhibitors and are resistant to oseltamivir. The efficacy of drug-resistant patients is not ideal; the use of ribavirin may cause adverse reactions such as nausea and vomiting, fatigue, headache, weakness, chest tightness, fever, chills, etc. In severe cases, leukopenia, hemolytic anemia, myocardial damage, and pancreatitis may occur. and other symptoms.

Traditional Chinese medicine can treat colds as a whole and shows unique efficacy in preventing and treating influenza with mild or no side effects. However, due to various reasons, it is limited to clinical dialectical treatment and individualized prescriptions, and the number of people who benefit is very limited.

Contents of the invention

In order to solve the deficiencies in the prior art, the present invention provides a traditional Chinese medicine extract composition for preventing and treating influenza, its preparation method and application, which can significantly reduce the mortality rate of influenza and the damage effect of influenza on the lungs, and broaden the selection of drugs for preventing and treating influenza. scope.

In order to achieve the above objects, the present invention adopts the following technical solutions:

The invention provides a traditional Chinese medicine extraction composition for preventing and treating influenza, which includes Angelica sinensis extract and Chuanxiong extract.

Preferably, the Angelicae Angelicae extract is Angelicae Sinensis volatile oil; the Ligusticum Chuanxiong extract is Ligusticum Chuanxiong volatile oil.

Preferably, the mass content of ferulic acid in the angelica volatile oil is greater than or equal to 1%, and the content ratio of ligustilide is greater than or equal to 30%.

Preferably, the mass content of ligustilide in the volatile oil of Ligusticum chuanxiong is not less than 10%, and the total amount of ligustilide and ligustilide A is not less than 20%.

The present invention also provides a traditional Chinese medicine extract composition for preventing and treating influenza, which includes the following components: Angelica sinensis extract, Ligusticum chuanxiong extract and auxiliary materials acceptable in medicines.

Preferably, the Angelica sinensis extract is extracted from the native drug of Angelica sinensis, and the Ligusticum chuanxiong extract is extracted from the native drug of Ligusticum chuanxiong. The mass ratio of the original drug of Angelica sinensis and the native drug of Ligusticum chuanxiong is calculated as (0.1~5): ( 0.2～10).

Preferably, the Ligusticum chuanxiong extract is Ligusticum chuanxiong volatile oil obtained by subcritical or supercritical extraction.

Preferably, the mass content of ligustilide in the Ligusticum chuanxiong extract is not less than 10%, and the total amount of ligustilide and ligustilide A is not less than 20%.

Preferably, the Angelicae Angelicae extract is Angelicae Sinensis volatile oil obtained by subcritical or supercritical extraction.

Preferably, the content of ferulic acid in the angelica extract is greater than or equal to 1%, and the content of ligustilide is greater than or equal to 30%.

Preferably, the ferulic acid in the composition is not less than 1%, and the total amount of ligustilide and ligustilide A is not less than 5%.

Preferably, the excipients are disintegrants, excipients, lubricants or binders.

The invention also provides a preparation method of the traditional Chinese medicine extract composition for preventing and treating influenza, which includes the following steps:

Preparation of Angelicae extract: Take Angelicae medicinal materials, crush and sieve, put into subcritical fluid extraction tank, add subcritical fluid, extract 2-3 times at 35-45℃, extraction time 35-45min, separate the extractant, and recover Angelicae volatile oil , that is, get;

Preparation of Ligusticum chuanxiong extract: Take Ligusticum chuanxiong medicinal materials, crush and sieve, put it into a subcritical fluid extraction tank, add subcritical fluid, extract 2-3 times at 35-45°C, extraction time 45-55 minutes, separate the extractant, and recover Ligusticum chuanxiong volatile oil , that’s it.

Preferably, the subcritical fluid is butane.

Preferably, the preparation method of the traditional Chinese medicine extract composition for preventing and treating influenza includes the following steps:

Preparation of Angelicae extract: Take Angelicae medicinal materials, crush them through a 40-mesh sieve, put them into a subcritical fluid extraction tank, add subcritical fluid, extract at 35°C for 45min, extract twice, separate the extractant, and recover Angelicae volatile oil to obtain;

Preparation of Ligusticum chuanxiong extract: Take Ligusticum chuanxiong medicinal material, grind it through a 40-mesh sieve, put it into a subcritical fluid extraction tank, add subcritical fluid, extract at 45°C for 45 minutes, extract twice, separate the extractant, and recover the volatile oil of Ligusticum chuanxiong.

Preferably, the preparation method of the traditional Chinese medicine extract composition for preventing and treating influenza is obtained by mixing Angelica sinensis extract, Ligusticum chuanxiong extract and acceptable auxiliary materials in medicines.

The present invention also provides a preparation of the traditional Chinese medicine extract composition, which is prepared into a pharmaceutically acceptable dosage form according to conventional processes.

Preferably, the preparation is an oral pharmaceutical preparation or an external preparation.

Preferably, the dosage form contains Ligusticum chuanxiong extract and Angelica sinensis extract.

The present invention also provides a use of the traditional Chinese medicine extract composition, the preparation method, or the preparation of the traditional Chinese medicine extract composition in the preparation of anti-influenza drugs.

More preferably, the application is specifically the protection of death caused by influenza virus fluid and the protection of lung damage caused by influenza virus fluid.

The beneficial effects of the present invention are:

1. The combination of Angelica sinensis extract and Ligusticum chuanxiong extract of the present invention has a good therapeutic effect on diseases caused by influenza virus. Research shows that the traditional Chinese medicine extract composition for preventing and treating influenza of the present invention can significantly extend the survival period of influenza virus mice and reduce the risk of influenza virus infection. The mortality rate of influenza virus mice; at the same time, it can significantly reduce the lung index of influenza virus mice. Different degrees of interstitial and alveolar inflammatory cell infiltration can be seen in lung specimens. The degree of lesions is reduced, and it can effectively inhibit lung damage caused by influenza virus.

2. The composition of the present invention fully considers the composition of the raw materials and the ratio between each raw material, so that the raw materials cooperate with each other and work together in a specific ratio, and have a significant synergistic effect in anti-influenza virus.

3. The present invention adopts new subcritical or supercritical extraction of native medicine to obtain extracts of volatile oils of Ligusticum chuanxiong and Angelica sinensis, which significantly improves the stability of its active ingredients and is conducive to the absorption and utilization of active ingredients. The process is stable and controllable, non-toxic and harmless, and can be produced industrially on a large scale.

Description of the drawings

Figure 1 shows the effect of traditional Chinese medicine extract composition on lung histopathology of influenza virus pneumonia mouse model (HE, ×100) (where, A: control; B: model; C: ribavirin; D: traditional Chinese medicine extract The composition preparation is 2.0 g/kg of the original drug; E: the traditional Chinese medicine extract composition preparation is 4.0 g/kg of the original drug; F: the traditional Chinese medicine extract composition preparation is 8.0 g/kg of the original drug).

Detailed ways

In order to enable those skilled in the art to better understand the technical solution of the invention, the invention will be further described in detail below in conjunction with specific embodiments.

The raw materials in the examples of the present invention are all commercially available, among which:

Ligusticum chuanxiong: purchased from Pengzhou and identified as the dried rhizome of Ligusticum chuanxiong Hort. The volatile oil is extracted subcritically. The yield of the traditional Chinese medicine extract is unstable and varies within a certain range. The experimentally determined yield in this example is 5.9%. Batch number: 2019080201, provided by the Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences.

Angelica sinensis: Purchased from Minxian County, Gansu Province, it was identified as the dried root of Angelicasinensis (Oliv.) Diels, an Umbelliferae plant. The volatile oil is extracted subcritically. The yield of the traditional Chinese medicine extract is unstable and varies within a certain range. The yield measured experimentally in this example is 2.2%. Batch number: 2019072901, provided by the Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences.

Edible oil: Arowana edible blended oil is used, batch number: 2HQ09, produced by Yihai Kerry (Chongqing) Grain and Oil Co., Ltd.

The auxiliary materials of the present invention are auxiliary materials commonly used in this field.

The dosage of the extract described in the embodiments of the present invention is converted by Angelica sinensis + Chuanxiong native drug g/kg. For example, "Chinese medicine extract composition preparation 2.0 native drug g/kg" means the native Angelica sinensis drug and Ligusticum chuanxiong native drug used for 1kg body weight. The medicine is 2g in total.

Example 1

Preparation of angelica extract: Take 1kg of angelica medicinal material, crush it and pass it through a 40-mesh sieve, put it into a subcritical fluid extraction tank (CBE-2×25L), add butane, extract at 45°C for 35 minutes, and extract 3 times , separate the extraction agent, recover angelica volatile oil, and detect that the mass content of ferulic acid is greater than or equal to 1.0%, and the mass content of ligustilide is greater than or equal to 30%;

Preparation of Ligusticum Chuanxiong extract: The dried whole medicinal materials of Ligusticum Chuanxiong were crushed with a grinder and passed through a 40-mesh sieve. Weighed 1kg, put it into a subcritical fluid extraction tank (CBE-2×25L), added butane, and heated at 35°C. , extract for 55 minutes, extract 3 times, separate the extraction agent, recover the volatile oil of Ligusticum chuanxiong, and detect that the content of ligustilide is not less than 10%, and the total amount of ligustilide and ligustilide A is not less than 20%;

The Angelica sinensis volatile oil and Ligusticum chuanxiong volatile oil prepared above (the mass ratio of the original medicinal materials is 1:1) is a traditional Chinese medicine extract composition. The ferulic acid in the composition is not less than 1%, and the content of ligustilide and ligustilide A is detected. The total amount is not less than 5%.

It can be prepared into soft capsules according to conventional processes, such as:

Add the above-mentioned angelica extract and Ligusticum chuanxiong extract to the soft capsule. The excipients account for 2% of the mass of the soft capsule, and the remainder is edible oil. Mix them to obtain the result.

Example 2

Preparation of angelica extract: Take 1kg of angelica medicinal material, crush it and pass it through a 40-mesh sieve, put it into a subcritical fluid extraction tank (CBE-2×25L), add butane, extract at 35°C for 45 minutes, and extract twice , separate the extraction agent, recover angelica volatile oil, and detect that the mass content of ferulic acid is greater than or equal to 1%, and the mass content of ligustilide is greater than or equal to 35%;

Preparation of Ligusticum chuanxiong extract: The dried whole medicinal material of Ligusticum chuanxiong was crushed with a pulverizer and passed through a 40-mesh sieve. Weighed 1kg, put it into a subcritical fluid extraction tank (CBE-2×25L), added butane, and heated at 45°C. , extract for 45 minutes, extract twice, separate the extraction agent, recover the volatile oil of Ligusticum chuanxiong, and detect that the content of ligustilide is not less than 12%, and the total amount of ligustilide and ligustilide A is not less than 20%;

The Angelica sinensis volatile oil and Ligusticum chuanxiong volatile oil prepared above (the mass ratio of the original medicinal materials is 1:1) are traditional Chinese medicine extract compositions. The ferulic acid in the composition is detected to be not less than 1%, ligustilide and ligustilide A. The total amount is not less than 5%.

It can be prepared into soft capsules according to conventional processes, such as:

Add the above-mentioned angelica extract and Ligusticum chuanxiong extract to the soft capsule. The auxiliary materials account for 3% of the mass of the soft capsule, and the remainder is edible oil. Mix them to obtain the soft capsule.

Example 3

Preparation of angelica extract: Take 2kg of angelica medicinal material, crush it and pass it through a 40 mesh sieve, put it into a subcritical fluid extraction tank (CBE-2×25L), add butane, extract at 35°C for 45 minutes, and extract twice , separate the extraction agent, recover angelica volatile oil, and detect that the mass content of ferulic acid is greater than or equal to 1%, and the mass content of ligustilide is greater than or equal to 35%;

Preparation of Ligusticum chuanxiong extract: The dried whole medicinal material of Ligusticum chuanxiong was crushed with a pulverizer and passed through a 40-mesh sieve. Weighed 2kg and put it into a subcritical fluid extraction tank (CBE-2×25L). Add butane and sieve at 45°C. , extract for 45 minutes, extract twice, separate the extraction agent, recover the volatile oil of Ligusticum chuanxiong, and detect that the content of ligustilide is not less than 10%, and the total amount of ligustilide and ligustilide A is not less than 20%;

The Angelica sinensis volatile oil and Ligusticum chuanxiong volatile oil prepared above (the mass ratio of the original medicinal materials is 1:1) are traditional Chinese medicine extract compositions. The ferulic acid in the composition is detected to be not less than 1%, ligustilide and ligustilide A. The total amount is not less than 5%.

It can be prepared into soft capsules according to conventional processes, such as:

Add the above-mentioned angelica extract and Ligusticum chuanxiong extract to the soft capsule. The auxiliary materials account for 5% of the mass of the soft capsule, and the remainder is edible oil. Mix them to obtain the soft capsule.

Example 4

Same as Example 1, except that the only difference is: 0.1kg of Angelica sinensis and 0.2kg of Ligusticum chuanxiong. The mass ratio of angelica volatile oil and Ligusticum chuanxiong volatile oil in the traditional Chinese medicine extract composition is 1:2 based on the original medicinal materials. Tablets are made according to conventional processes.

Example 5

Same as Example 1, the only difference is: 5 kg of Angelica sinensis and 10 kg of Ligusticum chuanxiong. The mass ratio of angelica volatile oil and Ligusticum chuanxiong volatile oil in the traditional Chinese medicine extract composition is 1:2 based on the original medicinal materials. Dripping pills are made according to conventional processes.

Effect Example

1. Experimental drugs:

Sodium chloride injection: Batch number: B21020205, produced by Sichuan Kelun Pharmaceutical Co., Ltd.

Ribavirin dispersible tablets: Batch number: 2012010, produced by Sinopharm Guorui Pharmaceutical Co., Ltd. Before use, prepare a suspension with the required concentration with 0.5% CMC solution for later use.

2.Test reagents

Formaldehyde solution: concentration 7.0%-40.0%, batch number: 20210415, produced by Chengdu Changlian Chemical Reagent Co., Ltd.;

1% guinea pig red blood cells: batch number 211122, produced by Guangzhou Hongquan Biotechnology Co., Ltd.

3. Experimental instruments

Analytical balance: model BP211D, produced by Sartorius, Germany; model YP1201, produced by Shanghai Liangping Instrument Co., Ltd.

Desktop high-speed refrigerated centrifuge: model TGL-16, produced by Changsha Xiangyi Centrifuge Instrument Co., Ltd.;

Fully automatic coagulation analyzer: Model: ExC810, produced by Mindray;

Biological safety cabinet: Model 1389, produced by Thermo Fisher Scientific;

Fully electric smart microscope: model Olympus BX63, produced by Olympus Japan. VarioskanFlash full-wavelength scanning multi-function reader: produced by Thermo Company of the United States.

4. Test cells/animals

Kunming mice, SPF grade, were provided by the Experimental Animal Center of the Sichuan Academy of Chinese Medical Sciences, production license number: SCXK (Sichuan)-2018-19.

Influenza virus strain, influenza A virus mouse lung-adapted strain A/PR/8/34 (H1N1), was purchased from the Institute of Virology, Chinese Academy of Preventive Medicine. After one round of passage and proliferation in chicken embryos, the hemagglutination titer was 1:512, and the LD50 for mice was 10-3.5/50μl. It was stored in a -80°C ultra-low temperature refrigerator.

5. Test environment

The experimental animals were kept in the barrier system of the Experimental Animal Center of the Sichuan Academy of Chinese Medical Sciences, with certificate number: SYXK (Sichuan)-2018-100, at a room temperature of 22-25°C, a humidity of 50±5%, and 12 hours of light and dark.

The preparation formula of the traditional Chinese medicine extract composition of the effect example is the same as that of Examples 1 to 3. When performing the effect experiment, there is no need to prepare a soft capsule, and only the traditional Chinese medicine extract composition and edible oil can be mixed.

Effect Example 1 The traditional Chinese medicine extract composition for preventing and treating influenza according to the present invention can effectively prevent the death of mice caused by influenza virus fluid. impact of death

Experimental grouping: Take Kunming mice with a body weight of 18-22g, 80 in total, half male and half female. After adaptive feeding for 2 days, they are randomly divided into 5 groups of 16 mice in each group according to body weight, which are model group and traditional Chinese medicine extract composition preparation. 8.0 native drug g/kg, traditional Chinese medicine extract composition preparation 4.0 native drug g/kg, traditional Chinese medicine extract composition preparation 2.0 native drug g/kg, ribavirin 0.1/kg.

Experimental process: Mice were administered intragastrically, once in the morning and afternoon every day, for 8 consecutive times; the model group was intragastrically administered an equal volume of edible oil, and the administration volume was 10ml/kg; after the third administration After 1 hour, the mouse was anesthetized and fixed on a fixed plate, so that the mouse's head was upward at a 45° angle to the horizontal. Then an incision was made in the middle of the neck to expose the trachea, and influenza A virus was injected into the trachea toward the lungs. Adapted strain A/PR/8/34 (H1N1) virus liquid (concentration is 10LD50=10-2.5) 0.05mL/mouse, then keep the mouse tilted for 2 minutes to allow the virus liquid to fully flow into the lungs; suture the surgical wound, disinfect and intramuscular injection Add 0.1 ml of 400,000 U/ml penicillin solution/mouse back to the cage until it wakes up, record the number of mice that died within 14 days after infection, and count the mortality of mice, as shown in Table 1.

Table 1 Effect of traditional Chinese medicine extract compositions for preventing and treating influenza on the mortality rate of influenza virus pneumonia mouse model

Compared with the model group: *P＜0.05, **P＜0.01, ***P＜0.001;

Mice died one after another 5 days after being infected with influenza virus. Without treatment, all died within 7 days after infection. As can be seen from Table 1, the traditional Chinese medicine extract composition for preventing and treating influenza of the present invention can significantly prolong the life of mice with influenza virus. The survival period is statistically significant when compared with the model group; 8.0 g/kg of the traditional Chinese medicine extract composition for preventing and treating influenza of the present invention can significantly reduce the mortality of influenza virus mice, and the difference is statistically significant when compared with the model group significance.

Effect Example 2 Effect of traditional Chinese medicine extract composition for preventing and treating influenza on lung injury in mice caused by influenza virus ring

Experimental grouping: 68 male Kunming mice with a body weight of 18-22g were randomly divided into 6 groups according to their weight, namely the control group, the model group, the Chinese medicine extract composition preparation 8.0 native medicine g/kg, and the Chinese medicine extract combination The pharmaceutical preparation is 4.0 g/kg of the original drug, the traditional Chinese medicine extract composition preparation is 2.0 g/kg of the original drug, and the ribavirin group is 0.1 mg/kg.

Experimental process: Mice were administered intragastrically once a day for 7 consecutive times; the model group and the control group were intragastrically administered equal volumes of edible oil, and the administration volume was both 10ml/kg. 1 hour after the third administration, After anesthesia, the mouse was fixed on a fixed plate so that the mouse's head was upward at a 45° angle to the horizontal. Then an incision was made in the middle of the neck to expose the trachea, and the mouse lung-adapted strain of influenza A virus was injected into the trachea toward the lungs. A/PR/8/34 (H1N1) virus liquid (concentration is 10LD50=10-2.5) 0.05mL/mouse, keep the mouse tilted for 2 minutes to allow the virus liquid to fully flow into the lungs, suture the incision and disinfect it; the control group is anesthetized and fixed Then make an incision in the middle of the neck on the fixed plate, inject an equal volume of normal saline, suture the incision and disinfect it; 1 hour after the last dose, kill the mouse, take the lung tissue, weigh the wet weight and calculate the organ index, and remove the lung tissue. The large leaves were fixed for pathological observation, see Table 2, Table 3 and Figure 1.

Table 2 Effects of traditional Chinese medicine extract compositions for preventing and treating influenza on lung index of influenza virus pneumonia mouse model

Compared with the model group: *P＜0.05, **P＜0.01, ***P＜0.001

On the fifth day after influenza virus infection, two mice died in the model group, and no deaths occurred in the remaining administration groups. As can be seen from Table 2, the lung index of mice increased significantly after being infected with influenza virus, and the difference was statistically significant compared with the control group. Scientific significance: Each dosage group of the traditional Chinese medicine extract composition of the present invention can significantly reduce the lung index of influenza virus mice, among which the traditional Chinese medicine extract composition preparation of 8.0 g/kg of native drug has the most significant effect, and the difference is statistically significant compared with the model group significance.

Table 3 Effects of traditional Chinese medicine extract compositions for preventing and treating influenza on lung histopathology of influenza virus pneumonia mouse model

It can be seen from Table 3 and Figure 1 that under the microscope, the lung specimens of the model group showed a large number of inflammatory cell infiltrates in the lung interstitium, alveolar septa and alveolar spaces, and the difference was statistically significant compared with the control group; the lungs of each dose group of the traditional Chinese medicine extract composition Different degrees of infiltration of pulmonary interstitium and alveolar septic cells were seen in all specimens, and the degree of lesions was lighter than that in the model group. Among them, the Chinese medicine extract composition preparation 8.0 g/kg of native drug had the most significant effect, and the difference was statistically significant compared with the model group.

The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limitations of the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. For those of ordinary skill in the art, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims (11)

1. A traditional Chinese medicine extract composition for preventing and treating influenza, which is characterized in that it includes Angelica sinensis extract and Chuanxiong extract.
2. The traditional Chinese medicine extraction composition for preventing and treating influenza according to claim 1, characterized in that the Angelica sinensis extract is Angelica sinensis volatile oil; the Ligusticum chuanxiong extract is Ligusticum chuanxiong volatile oil.
3. A traditional Chinese medicine extract composition for preventing and treating influenza, which is characterized in that it includes the following components: Angelica sinensis extract, Ligusticum chuanxiong extract and auxiliary materials acceptable in medicines.
4. The traditional Chinese medicine extract composition for preventing and treating influenza according to claim 3, wherein the angelica extract is extracted from the original medicine of Angelicae Sinensis, the Ligusticum chuanxiong extract is extracted from the original medicine of Ligusticum chuanxiong, and the original medicine of Angelica sinensis is extracted. The mass ratio of the crude drug and the crude drug of Chuanxiong is calculated as (0.1~5): (0.2~10).
5. The traditional Chinese medicine extract composition for preventing and treating influenza according to claim 3, characterized in that the Ligusticum chuanxiong extract is Ligusticum chuanxiong volatile oil obtained by subcritical or supercritical extraction.
6. The traditional Chinese medicine extract composition for preventing and treating influenza according to claim 3, characterized in that the mass content of ligustilide in the Ligusticum chuanxiong extract is not less than 10%, and the total content of ligustilide and ligustilide A The amount is not less than 20%.
7. The traditional Chinese medicine extract composition for preventing and treating influenza according to claim 3, characterized in that the Angelica sinensis extract is Angelica sinensis volatile oil obtained by subcritical or supercritical extraction.
8. The traditional Chinese medicine extract composition for preventing and treating influenza according to claim 3, characterized in that the mass content of ferulic acid in the angelica extract is greater than or equal to 1%, and the mass content of ligustilide is greater than or equal to 30 %.
9. The traditional Chinese medicine extract composition for preventing and treating influenza according to claim 3, wherein the auxiliary material is a disintegrant, excipient, lubricant or adhesive.
10. A preparation comprising the traditional Chinese medicine extract composition for preventing and treating influenza according to any one of claims 1 to 9, characterized in that it is prepared into a pharmaceutically acceptable dosage form according to conventional processes.
11. The application of the traditional Chinese medicine extract composition for preventing and treating influenza according to any one of claims 1 to 9 or the preparation of the composition according to claim 10 in the preparation of anti-influenza drugs.

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