WO2023196159A1 - Aliment pour poissons ou additif pour aliments pour poissons - Google Patents
Aliment pour poissons ou additif pour aliments pour poissons Download PDFInfo
- Publication number
- WO2023196159A1 WO2023196159A1 PCT/US2023/016913 US2023016913W WO2023196159A1 WO 2023196159 A1 WO2023196159 A1 WO 2023196159A1 US 2023016913 W US2023016913 W US 2023016913W WO 2023196159 A1 WO2023196159 A1 WO 2023196159A1
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- WO
- WIPO (PCT)
- Prior art keywords
- fish
- fish feed
- feed additive
- feed
- additive
- Prior art date
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 493
- 239000003674 animal food additive Substances 0.000 title claims abstract description 216
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 143
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 143
- 239000001301 oxygen Substances 0.000 claims abstract description 143
- 238000000034 method Methods 0.000 claims abstract description 122
- CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 claims abstract description 54
- DRBBFCLWYRJSJZ-UHFFFAOYSA-N N-phosphocreatine Chemical compound OC(=O)CN(C)C(=N)NP(O)(O)=O DRBBFCLWYRJSJZ-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000004475 Arginine Substances 0.000 claims abstract description 36
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000002253 acid Substances 0.000 claims abstract description 34
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- 229960003624 creatine Drugs 0.000 claims abstract description 27
- 229950007002 phosphocreatine Drugs 0.000 claims abstract description 26
- 208000024891 symptom Diseases 0.000 claims abstract description 9
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims abstract description 5
- 241000277331 Salmonidae Species 0.000 claims description 45
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- 238000002360 preparation method Methods 0.000 claims description 23
- 239000000203 mixture Substances 0.000 claims description 13
- 150000001450 anions Chemical class 0.000 claims description 12
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 229910001415 sodium ion Inorganic materials 0.000 claims description 11
- 230000004083 survival effect Effects 0.000 claims description 11
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- 239000000463 material Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
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- 244000144977 poultry Species 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000004949 second gill arch Anatomy 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
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- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
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- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000000385 Brassica napus var. napus Species 0.000 description 1
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- 108090000790 Enzymes Proteins 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
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- 206010021143 Hypoxia Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
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- 235000019764 Soybean Meal Nutrition 0.000 description 1
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- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
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- 239000001569 carbon dioxide Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006392 deoxygenation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
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- 238000012851 eutrophication Methods 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
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- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
Definitions
- Ocean deoxygenation is the reduction of the oxygen content of the oceans which can be caused by various factors such as anthropogenic emissions of carbon dioxide or eutrophication-driven excess production. It is manifest in the increasing number of coastal and estuarine hypoxic areas, or dead zones, and the expansion of oxygen minimum zones in the world’s oceans. The decrease in oxygen content of the oceans has been fairly rapid and poses a threat to all aerobic marine life, as well as to people who depend on marine life for nutrition or death. During low O2 events fish typically experience decreased feeding, metabolic stress, a reduction in grow th, and a reduction in animal welfare. There is a need for fish feed additives and methods that reduce or eliminate the symptoms of residing in a low oxygen environment.
- the present invention provides a fish feed additive that includes guanadinoacetic acid.
- the present invention provides a fish feed additive that includes guanadinoacetic acid that is 50 wt% to 99 wt% of the fish feed additive.
- the fish feed additive also includes arginine that is 50 wt% of the fish feed additive.
- the present invention provides a fish feed that includes a fish feed additive.
- the fish feed additive includes guanadinoacetic acid.
- the fish feed also includes a base feed component.
- the present invention provides a fish feed that includes a fish feed additive.
- the fish feed additive is 50 wt% to 100 wt% guanadinoacetic acid.
- the fish feed additive is 0.01 wt% to 5 wt% of the fish feed.
- the fish feed also includes a base feed component.
- the present invention provides a fish feed that includes guanadinoacetic acid.
- the guanadinoacetic acid is 0.01 wt% to 5% wt% of the fish feed.
- the fish feed also includes a base feed component.
- the present invention provides a method of forming the fish feed described herein.
- the method includes adding the fish feed additive described herein to a base feed component to form the fish feed.
- the present invention provides a method of feeding fish.
- the method includes feeding the fish the fish feed additive described herein or the fish feed described herein.
- the present invention provides a method of feeding salmonid fish.
- the method includes feeding the salmonid fish a feed composition including a base feed and including a feed additive that is 0.01 wt% to 5 wt% of the fish feed.
- the feed additive is 50 wt% to 100 wt% guanadinoacetic acid.
- the salmonid fish reside in an environment having an oxygen saturation of 30% to 85%.
- the method is effective to improve to maintain overall fish health, growth rate, survival rate, rate of feed consumption, gill efficiency, rate of damaged gill cell repair, gill cell energy production, gill cell energy storage, gill processes including oxygen absorption, gill processes including ionic homeostasis, gill processes including osmoregulation, gill processes including pH control, blood anion gap, blood pH, blood sodium ion concentration, or a combination thereof, as compared to the same fish in the same environment and fed a diet free of the fish feed additive.
- the present invention provides a fish feed additive for feeding to salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment.
- the fish feed additive includes arginine.
- the present invention provides a fish feed for feeding to salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment.
- the fish feed includes a fish feed additive that includes arginine.
- the fish feed also includes a base feed component.
- the present invention provides a method of feeding salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment.
- the method includes feeding the salmonids residing in the low oxygen environment a fish feed that includes arginine, a fish feed additive that includes arginine, or a combination thereof.
- the present invention provides a fish feed additive for feeding to salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment.
- the fish feed additive includes creatine and/or phosphocreatine.
- the present invention provides a fish feed for feeding to salmonids residing in a low oxygen environment or in preparation for residing in a low oxy gen environment.
- the fish feed includes a fish feed additive that includes creatine and/or phosphocreatine.
- the fish feed also includes a base feed component.
- the present invention provides a method of feeding salmonids residing in a low oxygen environment or in preparation for residing in a low oxy gen environment.
- the method includes feeding the salmonids residing in the low oxygen environment a fish feed that includes creatine and/or phosphocreatine, a fish feed additive that includes creatine and/or phosphocreatine, or a combination thereof.
- the fish feed additive of the present invention can have certain advantages over other fish feed additives and methods of using the same.
- the fish feed additive of the present invention and/or the fish feed including the same can be effective to improve or maintain overall fish health, growth rate, survival rate, rate of feed consumption, gill efficiency, rate of damaged gill cell repair, gill cell energy production, gill cell energy storage, gill processes including oxygen absorption, gill processes including ionic homeostasis, gill processes including osmoregulation, gill processes including pH control, blood anion gap, blood pH, blood sodium ion concentration, fish productivity, reduce overall stress, reduce susceptibility to secondary illness, or a combination thereof, as compared to the same fish in the same environment and fed a diet free of the fish feed additive.
- the fish feed additive of the present invention and/or the fish feed including the same when fed to a fish in a low oxygen environment, can be effective to reduce or eliminate one or more symptoms of the low oxygen environment on the fish.
- the fish feed additive of the present invention and/or the fish feed including the same may enable damaged gills to function more efficiently by supporting undamaged cells to maintain their gill function and prevent overall loss of fish condition while the damaged cells are being repaired.
- the fish feed additive of the present invention and/or the fish feed including the same may be used in reaction to or in preparation for sudden low oxygen events and/or gill damage, in reaction to known seasonal variations in oxygen concentration (e.g., administration may begin prior to the onset of low' oxygen conditions), and/or for programmed handling or disease treatment.
- FIG. 1 illustrates modelled weight gain of fish subjected to various feed/02 conditions, in accordance with various aspects.
- FIG. 2 illustrates the anion gap of blood of fish subjected to various feed/02 conditions, in accordance with various aspects.
- FIG. 3 illustrates blood pH of fish subjected to various feed/02 conditions, in accordance with various aspects.
- FIG. 4 illustrates blood sodium ion concentration of fish subjected to various feed/02 conditions, in accordance with various aspects.
- values expressed in a range format should be interpreted in a flexible manner to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range is explicitly recited.
- a range of “about 0.1% to about 5%” or “about 0.1% to 5%” should be interpreted to include not just about 0.1% to about 5%, but also the individual values (e.g., 1%, 2%, 3%, and 4%) and the sub-ranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, 3.3% to 4.4%) within the indicated range.
- the acts can be carried out in a specific order as recited herein.
- specific acts may be carried out in any order without departing from the principles of the invention, except when a temporal or operational sequence is explicitly recited.
- specified acts can be carried out concurrently unless explicit claim language recites that they be carried out separately or the plain meaning of the claims would require it.
- a claimed act of doing X and a claimed act of doing Y can be conducted simultaneously within a single operation, and the resulting process will fall within the literal scope of the claimed process.
- substantially refers to a majority of, or mostly, as in at least about 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.9%, 99.99%, or at least about 99.999% or more, or 100%.
- substantially free of can mean having none or having a trivial amount of, such that the amount of material present does not affect the material properties of the composition including the material, such that about 0 wt% to about 5 wt% of the composition is the material, or about 0 wt% to about 1 wt%, or about 5 wt% or less, or less than, equal to, or greater than about 4.5 wt%, 4, 3.5, 3, 2.5, 2, 1.5, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0. 1, 0.01, or about 0.001 wt% or less, or about 0 wt%.
- the present invention provides a fish feed additive that includes guanadinoacetic acid.
- the fish feed additive can be suitable for feeding to fish that reside in any aqueous environment, such as an environment having normal oxygen levels (e g., having an oxygen saturation of >95% to ⁇ 100%), or such as an environment having low oxygen levels.
- Low oxygen levels can include water conditions having an oxygen saturation of 10% to 95%, 30% to 85%, 50% to 70%, or less than or equal to 95% and greater than or equal to 10%, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, or 90%.
- An environment having normal oxygen levels can have an oxygen concentration of 6.5 mg/L or more, or 5.5 mg/L or more.
- An environment having low oxygen levels can have an oxygen concentration of 0. 1 mg/L to 6.5 mg/L, or 0. 1 mg/L to 5.5 mg/L, or 0.1 mg/L to 4 mg/L, or less than or equal to 6.5 mg/L and greater than or equal to 0.1 mg/L, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, or 6 mg/L.
- the fish feed additive can be suitable for feeding to any suitable type of fish.
- the fish can be a carp, anchovy, tuna, salmon, tilapia, Alaska pollack, catfish, cod, or a combination thereof.
- the fish can be a salmonid.
- the salmonid can be any suitable type of salmonid, such as Atlantic salmon, Chinook salmon, Chum salmon, Coho salmon, Masu salmon, Pink salmon, Sockeye salmon, Australian salmon, Danube salmon, Hawaiian salmon, Indian salmon, or a combination thereof.
- the fish feed additive be for feeding to a salmonids in a low oxygen environment.
- the fish feed additive When fed to fish, the fish feed additive can have a beneficial effect on the fish.
- the beneficial effect can occur when the fish feed additive is fed to fish in normal oxygen conditions or under low oxygen conditions.
- the fish feed additive When fed to fish, such as fish residing in a normal oxygen environment or under a low oxygen environment, the fish feed additive can be effective to improve to maintain overall fish health, growth rate, survival rate, rate of feed consumption, gill efficiency, rate of damaged gill cell repair, gill cell energy production, gill cell energy storage, gill processes including oxygen absorption, gill processes including ionic homeostasis, gill processes including osmoregulation, gill processes including pH control, blood anion gap, blood pH, blood sodium ion concentration, or a combination thereof, as compared to the same fish in the same environment and fed a diet free of the fish feed additive or fed a diet including less of the fish feed additive.
- the fish feed additive When fed to fish residing in a low oxygen environment or in preparation for
- the fish feed additive can be fed to the fish as any suitable proportion of the diet of the fish.
- the fish feed additive can be for feeding to the fish as 0.01 wt% to 5 wt% of the diet of the fish, 0.05 v % to 0.5 v .%, 0.
- Guanadinoacetic acid can be any suitable proportion of the fish feed additive.
- the guanadinoacetic acid can be 1 wt% to 100 wt% of the fish feed additive, 10 wt% to 100 wt%, 50 wt% to 100 wt%, 90 wt% to 100 wt%, or less than or equal to 100 wt% and greater than or equal to 1 wt%, 2, 4, 6, 8, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 92, 94, 96, 98, or 99 wt% of the fish feed additive.
- the fish feed additive can further include one or more secondary components.
- the one or more secondary components can form any suitable proportion of the fish feed additive, such as 0 wt% of the fish feed additive, or such as 0 wt% to 1 wt% of the fish feed additive, or 1 wt% to 99 wt% of the fish feed additive, 1 wt% to 90 wt%, 1 wt% to 50 wt%, 1 wt% to 20 wt%, 0.
- the fish feed additive can have any suitable weight ratio of the guanadinoacetic acid to the one or more secondary components, such as 99: 1 to 1 :99, 99: 1 to 1 : 1 , or less than or equal to 99: 1 and greater than or equal to 1:99, 1:95, 1:90, 1:85, 1:80, 1:75, 1:70, 1:65, 1:60, 1 :55, 1 :50, 1 :45, 1 :40, 1 :35, 1 :30, 1:25, 1:20, 1: 15, 1: 10, 1:5, 1 :4, 1:3, 1:2, 1 : 1, 2: 1, 3:1, 4: 1, 5: 1, 10: 1, 15: 1, 20: 1, 25: 1, 30: 1, 35: 1, 40: 1, 45: 1, 50: 1, 55:1, 60:1, 65:1, 70: 1, 75: 1, 80: 1, 85: 1, 90: 1, or 95:1.
- the one or more secondary components can include creatine, phosphocreatine, arginine, or a combination thereof.
- the one or more secondary components can include arginine.
- the fish feed additive can include guanadinoacetic acid and arginine, and can be substantially free of other materials.
- the fish feed additive can include guanadinoacetic acid and a secondary component that is arginine, creatine, and/or phosphocreatine, and can be substantially free of other materials. Fish feed.
- the present invention provides a fish feed that includes guanadinoacetic acid.
- the fish feed can be suitable for feeding to fish that reside in any environment, such as an environment having normal oxygen levels, or such as an environment having low oxygen levels.
- the fish feed can include the fish feed additive described herein including guanadinoacetic acid, and a base feed component.
- the fish feed additive can be any suitable proportion of the fish feed.
- the fish feed additive can be 0.01 wt% to 5 wt% of the fish feed, 0.05 wt% to 0.5 wt%, 0. 1 wt% to 0.2 wt%, or less than or equal to 5 wt% and greater than or equal to 0.01 wt%, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, 0.14, 0.15, 0.16, 0.18, 0.20, 0.25, 0.3, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.5, 3, 3.5, 4, or 4.5 wt% of the fish feed.
- the base feed component can be any suitable base feed.
- the base feed can be an aquafeed, such as a manufactured or artificial diet to supplement or to replace natural feeds in the aquaculture industry (e.g., the fish or salmonid industry ). These prepared feeds are most commonly produced in flake, pellet, or tablet form.
- the base feed is composed of several ingredients in various proportions complementing each other to form a nutntionally complete diet for the fish.
- the base feed can include one or more proteins, carbohydrates, fats, fibers, vitamins, minerals, phosphorus sources, or a combination thereof.
- the base feed can include wheat, wheat flour, soybean meal, fish meal, fish oil, poultry oil, vegetable oil (e g., from soybeans, rapeseeds, sunflower seeds, or flax seeds), cottonseed meal, peanut meal, canola meal, distillers grain with solubles, legume meal, binder attractant, additives, toxin absorbers, or a combination thereof.
- Additives can include enzymes, growth promoters, heath additives, or a combination thereof.
- Health additives can include immunostimulants, probiotics, vaccines, or a combination thereof.
- the base feed component can be any suitable proportion of the fish feed.
- the base feed component can be 95 wt% to 99.99 wt% of the fish feed, 99.5 wt% to 99.95 wt%, or less than or equal to 99.99 wt% and greater than or equal to 95 wt%, 95.5, 96, 96.5, 97, 97.5, 98, 98.5, 99, 99.2, 99.4, 99.6, 99.8, or 99.9 wt% of the fish feed.
- the guanadinoacetic acid of the fish feed additive can be any suitable proportion of the fish feed, such as 0.01 wt% to 5 wt% of the fish feed, 0.05 wt% to 0.5 wt% of the fish feed, or less than or equal to 5 wt% and greater than or equal to 0.01 wt%, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, 0.14, 0.15, 0.16, 0.18, 0.20, 0.25, 0.3, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.5, 3, 3.5, 4, or 4.5 wt% of the fish feed.
- the fish feed additive contains arginine
- the arginine from the fish feed additive can form any suitable proportion of the fish feed.
- arginine in the fish feed additive can be 0.001 wt% to 4 wt% of the fish feed, or 0.01 wt% to 2 wt% of the fish feed, or less than or equal to 4 wt% and greater than or equal to 0.001 wt%, 0.005, 0.01, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.2, 2.4, 2.6, 2.8, 3, 3.2, 3.4, 3.6, or 3.8 wt%.
- the arginine from the fish feed additive can form any suitable proportion of the fish feed.
- creatine and/or phosphocreatine in the fish feed additive can be 0.001 wt% to 4 wt% of the fish feed, or 0.01 wt% to 2 wt% of the fish feed, or less than or equal to 4 wt% and greater than or equal to 0.001 wt%, 0.005, 0.01, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.2, 2.4, 2.6, 2.8, 3, 3.2, 3.4, 3.6, or 3.8 wt%.
- the fish feed additive is vacuum coated on the base feed component.
- the vacuum coating can be performed by subjecting the base feed component to a vacuum, adding the fish feed additive thereto (optionally dispersed in a carrier liquid, such as an oil), and releasing the vacuum to cause the fish feed additive to be drawn within the pores of the base feed component.
- the oil can be any suitable oil, such as fish oil, poultry oil, or a combination thereof.
- the fish feed additive is combined with a base feed component and the combined mixture is extruded to form the fish feed.
- the fish feed can have any suitable physical form that is palatable to fish.
- the fish feed can have the form of a flake, tablet, pellet, or a combination thereof.
- the present invention provides a method of forming the fish feed described herein that includes a fish feed additive and a base feed component.
- the method can be any suitable method that forms the fish feed described herein.
- the method can include combining the fish feed additive and the base feed component together to form the fish feed.
- the method can include extruding a mixture of the fish feed additive and the base feed component to form the fish feed.
- the method includes vacuum coating the fish feed additive onto and into the base feed component.
- the vacuum coating can be performed by subjecting the base feed component to a vacuum, adding the fish feed additive thereto (optionally dispersed in a carrier liquid, such as an oil), and releasing the vacuum to cause the fish feed additive to be drawn within the pores of the base feed component.
- the present invention provides a method of feeding fish.
- the method includes feeding the fish the fish feed additive described herein and/or the fish feed described herein.
- the method can include feeding the fish while the fish reside in water having any suitable oxygen concentration, such as an environment having normal oxygen levels (e.g., having an oxygen saturation of >95% to ⁇ 100%), or such as an environment having low oxygen levels.
- Low oxygen levels can include water conditions having an oxygen saturation of 10% to 95%, 30% to 85%, 50% to 70%, or less than or equal to 95% and greater than or equal to 10%, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, or 90%.
- An environment having normal oxygen levels can have an oxygen concentration of 6.5 mg/L or more, or 5.5 mg/L or more.
- An environment having low oxygen levels can have an oxygen concentration of 0.1 mg/L to 6.5 mg/L, or 0.1 mg/L to 5.5 mg/L, or 0.1 mg/L to 4 mg/L, or less than or equal to 6.5 mg/L and greater than or equal to 0.1 mg/L, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, or 6 mg/L.
- the method can include feeding the fish in preparation for the fish to reside in water having such an oxygen concentration, such as 1-7 days ahead of time, 1-4 weeks ahead of time, or 1-6 months ahead of time.
- the fish feed additive can form any suitable proportion of the diet of the fish.
- the fish feed additive can be 0.01 wt% to 5 wt% of a diet of the fish, or 0.05 wt% to 0.5 wt%, or less than or equal to 5 wt% and greater than or equal to 0.01 wt%, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, 0.14, 0.15, 0.16, 0.18, 0.20, 0.25, 0.3, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.5, 3, 3.5, 4, or 4.5 wt% ofthe diet of the fish.
- the fish can be any suitable fish.
- the fish can be a carp, anchovy, tuna, salmon, tilapia, Alaska pollack, catfish, cod, or a combination thereof.
- the fish can be a salmonid.
- the salmonid can be any suitable type of salmonid, such as Atlantic salmon, Chinook salmon, Chum salmon, Coho salmon, Masu salmon, Pink salmon, Sockeye salmon, Australian salmon, Danube salmon, Hawaiian salmon, Indian salmon, or a combination thereof
- the method can have a beneficial effect on the fish.
- the beneficial effect can occur when the fish feed additive is fed to fish in normal oxygen conditions or under low oxygen conditions.
- the method can be effective to improve to maintain overall fish health, growth rate, survival rate, rate of feed consumption, gill efficiency, rate of damaged gill cell repair, gill cell energy production, gill cell energy storage, gill processes including oxygen absorption, gill processes including ionic homeostasis, gill processes including osmoregulation, gill processes including pH control, blood anion gap, blood pH, blood sodium ion concentration, or a combination thereof, as compared to the same fish in the same environment and fed a diet free of the fish feed additive or fed a diet including less of the fish feed additive.
- the method can be effective to reduce or eliminate one or more symptoms of the low oxygen environment on the fish.
- Fish feed additive including arginine, fish feed including same, and method of feeding fish the fish feed additive and/or the fish feed.
- the present invention provides a fish feed additive for feeding to fish such as salmonids.
- the fish can reside in an aqueous environment having normal oxygen levels or such as an aqueous environment having low oxygen levels.
- Low oxygen levels can include water conditions having an oxygen saturation of 10% to 95%, 30% to 85%, 50% to 70%, or less than or equal to 95% and greater than or equal to 10%, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, or 90%.
- An environment having normal oxygen levels can have an oxygen concentration of 6.5 mg/L or more, or 5.5 mg/L or more.
- An environment having low oxygen levels can have an oxygen concentration of 0.
- the fish feed additive can include arginine.
- arginine can be any suitable proportion of the fish feed additive, such as 1 wt% to 100 wt% of the fish feed additive, 1 wt% to 90 wt%, 1 wt% to 50 wt%, 1 wt% to 20 wt%, or less than or equal to 90 wt% and greater than or equal to 1 wt%, 2, 4, 6, 8, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 92, 94, 96, 97, or 98 wt% of the fish feed additive.
- the fish feed additive can be for feeding to fish at any suitable concentration of their diet, such wherein the fish feed additive is 0.01 wt% to 5 wt% of the diet of the fish, 0.05 wt% to 0.5 wt%, 0. 1 wt% to 0.2 wt%, or less than or equal to 5 wt% and greater than or equal to 0.01 wt%, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, 0.14, 0.15, 0.16, 0.18, 0.20, 0.25, 0.3, 0.4, 0.5, 0.6, 0.8, 1 , 1.2, 1.4, 1.6, 1.8, 2, 2.5, 3, 3.5, 4, or 4.5 wt% of the diet of the fish.
- the present invention provides a fish feed for feeding to fish such as salmonids, such as for feeding to fish in a low oxygen environment.
- the fish feed includes the fish feed additive that includes arginine.
- the fish feed also includes a base feed component.
- the fish feed can include any suitable amount of arginine, such as arginine at a concentration of 0.01 wt% to 5 wt% of fish feed, 0.05 wt% to 0.5 wt%, 0.
- the present invention provides a method of feeding fish such as salmonids, such wherein the fish reside in a low oxygen environment.
- the method includes feeding the salmonids residing in the low oxygen environment the fish feed of the present invention that includes arginine, or the fish feed additive of the present invention that includes arginine, or a combination thereof.
- the method can provide arginine at any suitable concentration in the diet of the fish, such as at a concentration of 0.01 wt% to 5 wt% of the diet of the fish, 0.05 wt% to 0.5 wt%, 0.1 wt% to 0.2 wt%, or less than or equal to 5 wt% and greater than or equal to 0.01 wt%, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, 0.14, 0.15, 0.16, 0.18, 0.20, 0.25, 0.3, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.5, 3, 3.5, 4, or 4.5 wt% of the diet of the fish.
- Fish feed additive including creatine and/or phosphocreatine, fish feed including same, and method of feeding fish the fish feed additive and/or the fish feed.
- the present invention provides a fish feed additive for feeding to fish such as salmonids.
- the fish can reside in an aqueous environment having normal oxygen levels or such as an aqueous environment having low oxygen levels.
- Low oxygen levels can include water conditions having an oxygen saturation of 10% to 95%, 30% to 85%, 50% to 70%, or less than or equal to 95% and greater than or equal to 10%, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, or 90%.
- An environment having normal oxygen levels can have an oxygen concentration of 6.5 mg/L or more, or 5.5 mg/L or more.
- An environment having low oxygen levels can have an oxygen concentration of 0.
- the fish feed additive can include creatine and/or phosphocreatine.
- creatine and/or phosphocreatine can be any suitable proportion of the fish feed additive, such as 1 wt% to 100 wt% of the fish feed additive, 1 wt% to 90 wt%, 1 wt% to 50 wt%, 1 wt% to 20 wt%, or less than or equal to 90 wt% and greater than or equal to 1 wt%, 2, 4, 6, 8, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 92, 94, 96, 97, or 98 wt% of the fish feed additive.
- the fish feed additive can be for feeding to fish at any suitable concentration of their diet, such wherein the fish feed additive is 0.01 wt% to 5 wt% of the diet of the fish, 0.05 wt% to 0.5 wt%, 0. 1 wt% to 0.2 wt%, or less than or equal to 5 wt% and greater than or equal to 0.01 wt%, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, 0.14, 0.15, 0.16, 0.18, 0.20, 0.25, 0.3, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.5, 3, 3.5, 4, or 4.5 wt% of the diet of the fish.
- the present invention provides a fish feed for feeding to fish such as salmonids, such as for feeding to fish in a low oxygen environment.
- the fish feed includes the fish feed additive that includes creatine and/or phosphocreatine.
- the fish feed also includes a base feed component.
- the fish feed can include any suitable amount of creatine and/or phosphocreatine, such as creatine and/or phosphocreatine at a concentration of 0.01 wt% to 5 wt% of fish feed, 0.05 wt% to 0.5 wt%, 0.1 wt% to 0.2 wt%, or less than or equal to 5 wt% and greater than or equal to 0.01 wt%, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, 0.14, 0.15, 0.16, 0.18, 0.20, 0.25, 0.3, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.5, 3, 3.5, 4, or 4.5 v .% of the fish feed.
- creatine and/or phosphocreatine such as creatine and/or phosphocreatine at a concentration of 0.01 wt% to 5 wt% of fish feed, 0.05 wt% to 0.5 wt%, 0.1 wt
- the present invention provides a method of feeding fish such as salmonids, such wherein the fish reside in a low oxygen environment.
- the method includes feeding the salmonids residing in the low oxygen environment the fish feed of the present invention that includes creatine and/or phosphocreatine, or the fish feed additive of the present invention that includes creatine and/or phosphocreatine, or a combination thereof.
- the method can provide creatine and/or phosphocreatine at any suitable concentration in the diet of the fish, such as at a concentration of 0.01 wt% to 5 wt% of the diet of the fish, 0.05 wt% to 0.5 wt%, 0.
- the present invention provides a method of feeding fish such as salmonids, such wherein the fish reside in a low oxygen environment.
- the method includes feeding the salmonids residing in the low oxygen environment the fish feed of the present invention that includes creatine and/or phosphocreatine, or the fish feed additive of the present invention that includes creatine and/or phosphocreatine, or a combination thereof.
- the method can provide creatine and/or phosphocreatine at any suitable concentration in the diet of the fish, such as at a concentration of 0.01 wt% to 5 wt% of the diet of the fish, 0.05 wt% to 0.5 wt%, 0.1 wt% to 0.2 wt%, or less than or equal to 5 wt% and greater than or equal to 0.01 wt%, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12, 0.14, 0.15, 0.16, 0.18, 0.20, 0.25, 0.3, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.5, 3, 3.5, 4, or 4.5 wt% of the diet of the fish.
- the second gill arch, from the right-hand side of the fish was removed and stored (10% buffered formalin) for later histological analysis, and the left second gill arch was removed and stored (rapid freezing -80 °C with liquid N2) for later qPCR analysis.
- Results showed that compared to standard Cargill Micro G feed at 60% O2 sat., the addition of CreAmino® at 0.15% w/w did not cause an improvement or reduction in fish grow th or condition when measured both at 28 days and 56 days after beginning the experimental feeding period.
- CreAmino alone did cause an improvement in certain blood parameters that may be beneficial for fish survival and wellbeing at low O2 conditions.
- Preliminary results using the same base feed but with 3 wt% total arginine in the feed instead of CreAmino caused a reduction in modelled growth, but also caused an improvement in certain blood parameters that may be beneficial for fish survival and well-being at low O2 conditions.
- FIG. 1 illustrates modelled weight gain of fish subjected to various feed/Ch conditions, in accordance with various aspects.
- FIG. 2 illustrates the anion gap of blood of fish subjected to various feed/Ch conditions, in accordance with various aspects.
- FIG. 3 illustrates blood pH of fish subjected to various feed/Ch conditions, in accordance with various aspects.
- FIG. 4 illustrates blood sodium ion concentration of fish subjected to various feed/02 conditions, in accordance with various aspects.
- Aspect 1 provides a fish feed additive comprising: guanadinoacetic acid.
- Aspect 2 provides the fish feed additive of Aspect 1, wherein the fish feed additive is for feeding to fish residing in a low oxygen environment or in preparation for residing in a low oxygen environment.
- Aspect 3 provides the fish feed additive of Aspect 2, wherein the fish residing in the low oxygen environment comprise salmonid fish.
- Aspect 4 provides the fish feed additive of any one of Aspects 2-3, wherein the low oxygen environment has an oxygen saturation of 10% to 95%.
- Aspect 5 provides the fish feed additive of any one of Aspects 2-4, wherein the low oxygen environment has an oxygen saturation of 30% to 85%.
- Aspect 6 provides the fish feed additive of any one of Aspects 2-5, wherein the low oxygen environment has an oxygen saturation of 50% to 70%.
- Aspect 7 provides the fish feed additive of any one of Aspects 1-6, wherein when fed to fish, the fish feed additive is effective to improve to maintain overall fish health, growth rate, survival rate, rate of feed consumption, gill efficiency, rate of damaged gill cell repair, gill cell energy production, gill cell energy storage, gill processes including oxygen absorption, gill processes including ionic homeostasis, gill processes including osmoregulation, gill processes including pH control, blood anion gap, blood pH, blood sodium ion concentration, or a combination thereof, as compared to the same fish in the same environment and fed a diet free of the fish feed additive.
- Aspect 8 provides the fish feed additive of any one of Aspects 1-7, wherein when fed to fish residing in a low oxygen environment or in preparation for residing in a low oxygen environment, the fish feed additive is effective to reduce or eliminate one or more symptoms of the low oxygen environment on the fish.
- Aspect 9 provides the fish feed additive of any one of Aspects 1-8, wherein when fed to fish residing in a low oxygen environment or in preparation for residing in a low oxy gen environment, the fish feed additive is effective to maintain or improve overall fish health, growth rate, survival rate, rate of feed consumption, gill efficiency, rate of damaged gill cell repair, gill cell energy production, gill cell energy storage, gill processes including oxygen absorption, gill processes including ionic homeostasis, gill processes including osmoregulation, gill processes including pH control, blood anion gap, blood pH, blood sodium ion concentration, or a combination thereof, as compared to the same fish in the same environment and fed a diet free of the fish feed additive.
- Aspect 10 provides the fish feed additive of any one of Aspects 1-9, wherein the fish feed additive is for feeding to carp, anchovy , tuna, salmon, tilapia, Alaska pollack, catfish, cod, or a combination thereof
- Aspect 11 provides the fish feed additive of any one of Aspects 1-10, wherein the fish feed additive is for feeding to salmonid fish.
- Aspect 12 provides the fish feed additive of any one of Aspects 1-11, wherein the fish feed additive is for feeding to a salmonid fish comprising Atlantic salmon, Chinook salmon, Chum salmon, Coho salmon, Masu salmon, Pink salmon, Sockeye salmon, Australian salmon, Danube salmon, Hawaiian salmon, Indian salmon, or a combination thereof.
- Aspect 13 provides the fish feed additive of any one of Aspects 1-12, wherein the fish feed additive is for feeding to fish as 0.01 wt% to 5 wt% of a diet of the fish.
- Aspect 14 provides the fish feed additive of any one of Aspects 1-13, wherein the fish feed additive is for feeding to fish as 0.05 wt% to 0.5 wt% of a diet of the fish.
- Aspect 15 provides the fish feed additive of any one of Aspects 1-14, wherein the fish feed additive is for feeding to fish as 0. 1 wt% to 0.2 wt% of a diet of the fish.
- Aspect 16 provides the fish feed additive of any one of Aspects 1-15, wherein the guanadinoacetic acid is 1 wt% to 100 wt% of the fish feed additive.
- Aspect 17 provides the fish feed additive of any one of Aspects 1-16, wherein the guanadinoacetic acid is 90 wt% to 100 wt% of the fish feed additive.
- Aspect 18 provides the fish feed additive of any one of Aspects 1-17, further comprising one or more secondary components.
- Aspect 19 provides the fish feed additive of Aspect 18, wherein the one or more secondary components are 1 wt% to 90 wt% of the fish feed additive.
- Aspect 20 provides the fish feed additive of any one of Aspects 18-19, wherein the one or more secondary components are 1 wt% to 50 wt% of the fish feed additive.
- Aspect 21 provides the fish feed additive of any one of Aspects 18-20, wherein the one or more secondary components are 1 wt% to 20 wt% of the fish feed additive.
- Aspect 22 provides the fish feed additive of any one of Aspects 18-21, wherein the fish feed additive has a weight ratio of the guanadinoacetic acid to the one or more secondary components of 99: 1 to 1:99.
- Aspect 23 provides the fish feed additive of any one of Aspects 18-22, wherein the fish feed additive has a weight ratio of the guanadinoacetic acid to the one or more secondary components of 99: 1 to 1: 1.
- Aspect 24 provides the fish feed additive of any one of Aspects 18-23, wherein the secondary component comprises creatine, phosphocreatine, arginine, or a combination thereof.
- Aspect 25 provides the fish feed additive of any one of Aspects 18-24, wherein the secondary component comprises arginine.
- Aspect 26 provides the fish feed additive of Aspect 25, wherein the fish feed additive has a weight ratio of the guanadinoacetic acid to arginine of 99: 1 to 1 :99.
- Aspect 27 provides the fish feed additive of any one of Aspects 25-26, wherein the fish feed additive has a weight ratio of the guanadinoacetic acid to arginine of 99:1 to 1 : 1.
- Aspect 28 provides a fish feed additive comprising: guanadinoacetic acid that is 50 wt% to 99 wt% of the fish feed additive; and arginine that is 1 wt% to 50 wt% of the fish feed additive.
- Aspect 29 provides a fish feed comprising: the fish feed additive of any one of Aspects 1-28; and a base feed component.
- Aspect 30 provides the fish feed of Aspect 29, wherein the fish feed additive is 0.01 wt% to 5 wt% of the fish feed.
- Aspect 31 provides the fish feed of any one of Aspects 29-30, wherein the fish feed additive is 0.05 wt% to 0.5 wt% of the fish feed.
- Aspect 32 provides the fish feed of any one of Aspects 29-31, wherein the fish feed additive is 0. 1 wt% to 0.2 wt% of the fish feed.
- Aspect 33 provides the fish feed of any one of Aspects 29-32, wherein the base feed component is 95 wt% to 99.99 wt% of the fish feed.
- Aspect 34 provides the fish feed of any one of Aspects 29-33, wherein the base feed component is 99.5 wt% to 99.95 wt% of the fish feed.
- Aspect 35 provides the fish feed of any one of Aspects 29-34, wherein the guanadinoacetic acid of the fish feed additive is 0.01 wt% to 5 wt% of the fish feed.
- Aspect 36 provides the fish feed of any one of Aspects 29-35, wherein the guanadinoacetic acid of the fish feed additive is 0.05 wt% to 0.5 wt% of the fish feed.
- Aspect 37 provides the fish feed of any one of Aspects 29-36, wherein arginine from the fish feed additive is 0.001 wt% to 4 wt% of the fish feed.
- Aspect 38 provides the fish feed of any one of Aspects 29-37, wherein arginine from the fish feed additive is 0.01 wt% to 2 wt% of the fish feed.
- Aspect 39 provides the fish feed of any one of Aspects 29-38, wherein the fish feed additive is vacuum coated on the base feed component, or wherein a mixture of the fish feed additive and the base feed component are extruded.
- Aspect 40 provides the fish feed of any one of Aspects 29-39, wherein a mixture of the fish feed additive and a carrier liquid is vacuum coated on the base feed component.
- Aspect 41 provides the fish feed of Aspect 40, wherein the carrier liquid comprises an oil.
- Aspect 42 provides the fish feed of any one of Aspects 29-41, wherein the base feed component comprises proteins, carbohydrates, fat, fiber, vitamins, minerals, a phosphorus source, or a combination thereof.
- Aspect 43 provides the fish feed of any one of Aspects 29-42, wherein the fish feed has the form of a flake, tablet, pellet, or a combination thereof.
- Aspect 43.5 provides a fish feed comprising: guanadinoacetic acid that is 0.01 wt% to 5 wt% of the fish feed; and a base feed component.
- Aspect 44 provides a fish feed comprising: a fish feed additive that is 50 wt% to 100 wt% guanadinoacetic acid, wherein the fish feed additive is 0.01 wt% to 5 wt% of the fish feed; and a base feed component
- Aspect 45 provides a method of forming the fish feed of any one of Aspects 29-44, the method comprising: adding the fish feed additive of any one of Aspects 1-28 to the base feed component to form the fish feed.
- Aspect 46 provides the method of Aspect 45, wherein adding the fish feed additive to the base feed component comprises vacuum coating an oil composition comprising the fish feed additive onto the base feed component, or wherein adding the fish feed additive to the base feed component comprises forming a mixture of the fish feed additive and the base feed component and the method further comprises extruding the mixture to form the fish feed.
- Aspect 47 provides a method of feeding fish, the method comprising: feeding the fish the fish feed additive of any one of Aspects 1-28 and/or the fish feed of any one of Aspects 29-44.
- Aspect 48 provides the method of Aspect 47, wherein the fish reside in an environment having an oxygen saturation of 10% to 95%.
- Aspect 49 provides the method of any one of Aspects 47-48, wherein the fish reside in an environment having an oxygen saturation of 30% to 85%.
- Aspect 50 provides the method of any one of Aspects 47-49, wherein the fish reside in an environment having an oxygen saturation of 50% to 70%.
- Aspect 51 provides the method of any one of Aspects 47-50, wherein the fish feed additive is 0.01 wt% to 5 wt% of a diet of the fish.
- Aspect 52 provides the method of any one of Aspects 47-51, wherein the fish feed additive is 0.05 wt% to 0.5 wt% of a diet of the fish.
- Aspect 53 provides the method of any one of Aspects 47-52, wherein the fish are carp, anchovy, tuna, salmon, tilapia, Alaska pollack, catfish, cod, or a combination thereof.
- Aspect 54 provides the method of any one of Aspects 47-53, wherein the fish are salmonids.
- Aspect 55 provides the method of any one of Aspects 47-54, wherein the method is effective to reduce or eliminate one or more symptoms of the low oxygen environment on the fish.
- Aspect 56 provides the method of any one of Aspects 47-55, wherein the method is effective to improve to maintain overall fish health, growth rate, survival rate, rate of feed consumption, gill efficiency, rate of damaged gill cell repair, gill cell energy production, gill cell energy' storage, gill processes including oxygen absorption, gill processes including ionic homeostasis, gill processes including osmoregulation, gill processes including pH control, blood anion gap, blood pH, blood sodium ion concentration, or a combination thereof, as compared to the same fish in the same environment and fed a diet free of the fish feed additive.
- Aspect 57 provides a method of feeding salmonid fish, the method comprising: feeding the salmonid fish a feed composition comprising a base feed and comprising a feed additive that is 0.01 wt% to 5 wt% of the fish feed, wherein the feed additive is 50 wt% to 100 wt% guanadinoacetic acid; wherein the salmonid fish reside in an environment having an oxygen saturation of 30% to 85%, and wherein the method is effective to improve to maintain overall fish health, growth rate, survival rate, rate of feed consumption, gill efficiency, rate of damaged gill cell repair, gill cell energy production, gill cell energy storage, gill processes including oxygen absorption, gill processes including ionic homeostasis, gill processes including osmoregulation, gill processes including pH control, blood anion gap, blood pH, blood sodium ion concentration, or a combination thereof, as compared to the same fish in the same environment and fed a diet free of the fish feed additive.
- Aspect 58 provides a fish feed additive for feeding to salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment, the fish feed additive comprising arginine.
- Aspect 59 provides a fish feed for feeding to salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment, the fish feed comprising: the fish feed additive of Aspect 58; and a base feed component.
- Aspect 60 provides a method of feeding salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment, the method comprising: feeding the salmonids residing in the low oxygen environment the fish feed of Aspect 59, the fish feed additive of Aspect 58, or a combination thereof.
- Aspect 61 provides a fish feed additive for feeding to salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment, the fish feed additive comprising creatine and/or phosphocreatine.
- Aspect 62 provides a fish feed for feeding to salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment, the fish feed comprising: the fish feed additive of Aspect 61; and a base feed component
- Aspect 63 provides a method of feeding salmonids residing in a low oxygen environment or in preparation for residing in a low oxygen environment, the method comprising: feeding the salmonids residing in the low oxygen environment the fish feed of Aspect 61, the fish feed additive of Aspect 62, or a combination thereof.
- Aspect 61 provides the fish feed additive, fish feed, method of forming the fish feed, or method of feeding fish of any one or any combination of Aspects 1-60 optionally configured such that all elements or options recited are available to use or select from.
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- Marine Sciences & Fisheries (AREA)
- Insects & Arthropods (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
L'invention concerne un additif alimentaire pour poissons ou un aliment pour poissons qui comprend de l'acide guanadinoacétique, de l'arginine, de la créatine et/ou de la phosphocréatine, et des procédés de fabrication et d'utilisation de l'additif alimentaire pour poissons ou de l'aliment pour poissons. Lorsqu'il est donné à des poissons se trouvant dans des conditions de faible teneur en oxygène, l'additif alimentaire pour poissons ou l'aliment pour poissons peut être efficace pour réduire ou éliminer un ou plusieurs symptômes de l'environnement à faible teneur en oxygène sur le poisson.
Applications Claiming Priority (2)
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US202263329269P | 2022-04-08 | 2022-04-08 | |
US63/329,269 | 2022-04-08 |
Publications (1)
Publication Number | Publication Date |
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WO2023196159A1 true WO2023196159A1 (fr) | 2023-10-12 |
Family
ID=86099800
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/US2023/016913 WO2023196159A1 (fr) | 2022-04-08 | 2023-03-30 | Aliment pour poissons ou additif pour aliments pour poissons |
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WO (1) | WO2023196159A1 (fr) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2569269A1 (fr) * | 2004-06-09 | 2005-12-22 | Degussa Ag | Acide guanidinoacetique servant de complement pour aliments destines aux animaux |
WO2019185408A1 (fr) * | 2018-03-27 | 2019-10-03 | Evonik Degussa Gmbh | Utilisation de l'acide guanidinoacétique et/ou de la créatine en aquaculture |
CN113208012A (zh) * | 2021-05-19 | 2021-08-06 | 湖北嘉康生物科技有限公司 | 一种加州鲈诱食促消化功能性饲料添加剂 |
-
2023
- 2023-03-30 WO PCT/US2023/016913 patent/WO2023196159A1/fr unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2569269A1 (fr) * | 2004-06-09 | 2005-12-22 | Degussa Ag | Acide guanidinoacetique servant de complement pour aliments destines aux animaux |
WO2019185408A1 (fr) * | 2018-03-27 | 2019-10-03 | Evonik Degussa Gmbh | Utilisation de l'acide guanidinoacétique et/ou de la créatine en aquaculture |
CN113208012A (zh) * | 2021-05-19 | 2021-08-06 | 湖北嘉康生物科技有限公司 | 一种加州鲈诱食促消化功能性饲料添加剂 |
Non-Patent Citations (3)
Title |
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AKIYAMA ET AL: "Amino acid requirements of chum salmon fry and supplementation of amino acids to diet", 1 January 1993 (1993-01-01), pages 35 - 48, XP008182867, Retrieved from the Internet <URL:https://docs.lib.noaa.gov/noaa_documents/NMFS/Aquaculture/Proceedings_20th_US_Japan_Meeting.pdf> * |
MCFARLANE W.J ET AL: "Creatine supplementation affects sprint endurance in juvenile rainbow trout", COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART A, MOLECULAR AND INTEGRATIVE PHYSIOLOGY, vol. 130, no. 4, 1 November 2001 (2001-11-01), US, pages 857 - 866, XP093052418, ISSN: 1095-6433, DOI: 10.1016/S1095-6433(01)00448-2 * |
VARGHESE TINCY ET AL: "Dietary arginine attenuates hypoxia- induced HIF expression, metabolic responses and oxidative stress in Indian Major Carp, Cirrhinus mrigala", COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY PART B: BIOCHEMISTRY AND MOLECULAR BIOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 259, 10 January 2022 (2022-01-10), XP086973736, ISSN: 1096-4959, [retrieved on 20220110], DOI: 10.1016/J.CBPB.2022.110714 * |
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