WO2023178498A1 - 封闭型细胞分离操作装置 - Google Patents

封闭型细胞分离操作装置 Download PDF

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Publication number
WO2023178498A1
WO2023178498A1 PCT/CN2022/082170 CN2022082170W WO2023178498A1 WO 2023178498 A1 WO2023178498 A1 WO 2023178498A1 CN 2022082170 W CN2022082170 W CN 2022082170W WO 2023178498 A1 WO2023178498 A1 WO 2023178498A1
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Prior art keywords
tube body
pipe
tube
closed cell
cell separation
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PCT/CN2022/082170
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English (en)
French (fr)
Inventor
苏鸿麟
申静懿
王复辉
谢佳颖
Original Assignee
通用干细胞股份有限公司
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Application filed by 通用干细胞股份有限公司 filed Critical 通用干细胞股份有限公司
Priority to AU2022449081A priority Critical patent/AU2022449081A1/en
Priority to PCT/CN2022/082170 priority patent/WO2023178498A1/zh
Publication of WO2023178498A1 publication Critical patent/WO2023178498A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/08—Apparatus for tissue disaggregation

Definitions

  • the present invention belongs to the technical field of cell centrifugal separation, and more specifically relates to a closed cell separation operating device that can provide a closed and pollution-free operating environment for supernatant separation during and after centrifugation.
  • Centrifugation is a technique commonly used in cell-related research and testing. The purpose is to separate cells from bone marrow concentrate (BMAC), cerebrospinal fluid (CSF), peripheral blood, urine, ascites, cell culture media and other specimens.
  • BMAC bone marrow concentrate
  • CSF cerebrospinal fluid
  • a centrifuge tube is a necessary tool in centrifugal separation operations. Its common form is a test tube with a lid, such as a centrifuge tube sold under the name Nunc (Thermo Fisher Scientific Company of the United States). According to the existing technology, the fluid to be centrifuged must be poured into a centrifuge tube, and then the centrifuge tube is sealed and placed in a centrifuge for processing. After centrifugation, if you want to remove the separated supernatant (such as plasma when whole blood is centrifuged) and obtain the precipitate (such as blood cells when whole blood is centrifuged), or vice versa, the operator must open the test tube and pour out the supernatant. into a spare container so that the two parts can be separated.
  • the separated supernatant such as plasma when whole blood is centrifuged
  • the precipitate such as blood cells when whole blood is centrifuged
  • the main purpose of the present invention is to provide a closed cell separation operating device, which can provide a closed pollution-free environment for separation of the supernatant during or after centrifugation, while avoiding the risk of specimen splashing. and eliminates the need to purchase expensive laboratory equipment.
  • the closed cell separation operating device includes: a first tube body, which is a cylindrical container with a circumferential wall, the first tube body has a closed top and passes through An air hole and an inlet are connected to the outside world in a closable manner, wherein the air hole is an air hole that allows gas to pass in and out of the first tube body, and the inlet is an air hole that allows fluid from an external fluid source to enter the first tube.
  • the first tube body has a closed bottom and is connected to the outside world in a closable manner through an outlet formed in the center;
  • a second tube body is a cylindrical container with a circumferential wall, and the second tube body has a closed bottom.
  • the tube body has a closed top and is connected to the outside world in a closable manner through an air hole and an inlet, wherein the air hole is an air hole for gas to pass in and out of the second tube body, and the inlet is for gas to pass from the first tube body to the second tube body. At least part of the fluid at the outlet of the pipe body enters the inlet of the second pipe body.
  • the second pipe body has a closed bottom and is connected to the outside world in a closable manner through an outlet formed in the center; and a connecting pipe is provided Between the outlet of the first pipe body and the inlet of the second pipe body, the first pipe body is connected to the second pipe body through the connecting pipe.
  • peripheral wall of the first pipe body or/and the second pipe body extends downward toward the bottom of the pipe body, the peripheral wall and the bottom form a pipe fitting storage space around the outlet of the pipe body, and at least part of the connecting pipe or/ And at least part of the extension pipe can be coiled around the outlet and stored in the pipe fitting storage space.
  • the closed cell separation operating device also includes an extension tube provided at the outlet of the second tube body, and the second tube body is connected to another end of the closed cell separation operating device through the extension tube.
  • One of the first pipe bodies is connected.
  • At least one lower edge of the peripheral walls of the first tube body and the second tube body is formed with a notch for the connecting tube to pass, so when the first tube body When the second pipe body stands on a flat surface, a passage is left in the pipe fitting storage space of the first pipe body/second pipe body for the connecting pipe to pass through.
  • At least one of the connecting tube and the extension tube is equipped with a valve for closing or opening the connecting tube or/and the extension tube, and the valve is At least one selected from the group consisting of pipe wrenches, flow control valves, multi-way faucet connectors and fluid switching valves.
  • the bottom of at least one of the first tube body and the second tube body has a downwardly converging conical structure, the diameter of which is tapered and the bottom of the first tube body is tapered.
  • the outlet of the body or/and the second pipe body reaches a minimum.
  • At least one of the pores of the first tube body and the second tube body is equipped with a filter for filtering air passing through the pores.
  • the closed cell separation operating device further includes an air pressure source provided at the air hole in at least one of the first and second tube bodies, and the air pressure source is used to push air into or extract air from the third tube.
  • the air pressure source is an air pump or an air injector.
  • At least one of the inlet and the air hole of the first tube body and the second tube body is provided with a sealing member that is sealed in an airtight manner.
  • At least one of the inlet and the air hole of the first tube body and the second tube body is further provided with a conduit equipped with a valve.
  • the valve component is at least one selected from the group consisting of pipe wrenches, flow control valves, multi-way faucet connectors and fluid switching valves.
  • the closed cell separation operating device of the present invention can provide a closed pollution-free environment for supernatant separation during or after centrifugation, while avoiding the risk of specimen splashing and eliminating the need to purchase expensive laboratory equipment.
  • Figure 1 is a three-dimensional perspective view of a closed cell separation operating device in an embodiment of the present invention
  • Figures 2 to 4 are application cross-sectional views of the closed cell separation operating device of the present invention, illustrating the operating status of the closed cell separation operating device during centrifugation and supernatant separation after centrifugation;
  • Figure 5 is a three-dimensional perspective view of another embodiment of a closed cell separation operating device, in which two sets of closed cell separation operating devices of the present invention are connected in series;
  • Figure 6 is a three-dimensional perspective view of a closed cell separation operating device in another embodiment of the present invention.
  • first pipe body 11, 21, opening; 121, 221 outlet; 12, 22, bottom; 122, 222, peripheral wall; 123, 223, pipe fitting storage space; 124, 224, notch; 13, First cap; 131, 231, air hole; 132, 232, inlet; 20, second pipe body; 23, second cap; 24, connecting pipe; 30, breathable pipe; 31, filter; 40, liquid inlet Pipe; 50, extension pipe; 60, valve; 70, air pressure source; 80, seal; 90, second breathable pipe; 91, additional filter.
  • a closed cell separation operating device mainly includes a first tube body 10 with a first cap 13, and a second tube 20 with a second cap 23. and a connecting pipe 24 connecting the first pipe body 10 and the second pipe body 20 .
  • the first tube body 10 and the second tube body 20 are substantially the same in structure, for the sake of simplicity, only one of them will be described below.
  • the first tube body 10 or the second tube body 20 is a cylindrical container surrounded by a peripheral wall 122 or 222.
  • the first tube body 10 or the second tube body 20 has a top that is completely open to the outside through the opening 11 or 21, and a bottom 12 or 22 that is closed but has an outlet 121 or 221 formed in the center to the outside.
  • the bottom 12 or 22 has a funnel-shaped structure that tapers downward, and its diameter gradually decreases from top to bottom, reaching a minimum around the outlet 121 or 221 .
  • the peripheral wall 122 or 222 of the first pipe body 10 or the second pipe body 20 extends downward beyond the closed bottom 12 or 22, thus forming a pipe storage space 123 or 223 together with the funnel-shaped bottom 12 or 22 around the outlet 121 or 221. .
  • a notch 124 or 224 is also formed on the lower edge of the peripheral wall 122 or 222. Therefore, when the first pipe body 10 or the second pipe body 20 stands on a flat surface, a channel will be formed for the pipe fitting storage space 123 or 223.
  • the size of the first tube body 10 or the second tube body 20 can be similar to existing centrifuge tubes or blood collection tubes, and therefore can be compatible with existing centrifuges.
  • first cap 13 and the second cap 23 are substantially the same in structure, for the sake of simplicity, only one of them will be described below.
  • the first cap 13 or the second cap 23 can cover the top of the first pipe body 10 or the second pipe body 20 from top to bottom, and is provided with an air hole 131 or 231 and an inlet 132 or 232.
  • the vent 131 or 231 and the inlet 132 or 232 are each configured to be removably connected to a conduit when in use and/or to be removably hermetically sealed by the seal 80 when not in use. .
  • the cap 13 or 23 on the first pipe body 10 or the second pipe body 20 is provided with an air hole 131 or 231 and an inlet 132 or 232
  • the closed type of the present invention The air hole 131 or 231 and the inlet 132 or 232 of the cell separation operating device can be directly provided on the closed top of the first tube body 10 or the second tube body 20 instead of using the cap 13 or 23 to cover the first tube body. 10 or the second tube body 20.
  • the connecting pipe 24 is disposed between the outlet 121 of the first pipe body 10 and the inlet 232 of the second pipe body 20 so that the first pipe body 10 and the second pipe body 20 are in fluid communication.
  • the connecting pipe 24 can be partially coiled around the outlet 121 of the first pipe body 10 and properly stored in the pipe fitting storage space 123 of the first pipe body 10 . Since the notch 124 is formed on the peripheral wall 122 of the first pipe body 10, the connecting pipe 24 can extend between the first pipe body 10 and the second pipe body 20 without affecting the first pipe body 10 standing firmly on any plane.
  • the connecting pipe 24 is also provided with a valve 60 for closing or opening the connecting pipe 24 .
  • the inlet 132 of the first tube body 10 is connected to the liquid inlet pipe from the source of the fluid to be processed (such as a blood bag, a blood collection needle or a specimen tube, not shown). 40 connections.
  • the air hole 131 of the first tube body 10 remains open, so the fluid to be processed (eg, whole blood) can enter the first tube body 10, as shown in FIG. 2 .
  • the air hole 131 of the first pipe body 10 may be provided with a filter 31 to prevent foreign matter from entering the first pipe body 10 and ensure a pollution-free operating environment. Specifically, as shown in FIG.
  • the ventilation tube 30 provided with the filter 31 is connected to the air hole 131 of the first tube body 10 .
  • the ventilation tube 30 can also be controlled by the valve member 60 to open or close.
  • the sealing member 80 can be used to directly seal the inlet 132 of the first tube body 10 airtightly, or the sealing member 80 can be used to seal the inlet 132 that is still connected to the first tube body 10.
  • the liquid inlet pipe 40 is airtightly closed, as shown in Figure 1.
  • the sealing member 80 can be used to directly seal the air hole 131 of the first tube body 10 airtightly, or the sealing member 80 can be used to airtightly cover the ventilation tube 30 that is still connected to the air hole 131, as shown in Figure 1 .
  • the connecting pipe 24, the breathable pipe 30 and the liquid inlet pipe 40 are closed by their respective valves 60, the inside of the first pipe body 10 is separated from the outside world, forming a closed environment. Similarly, at this time, the air hole 231 and the outlet 221 of the second tube body 20 are also closed, so they are isolated from the outside world.
  • the entire closed cell separation operating device can then be moved into a centrifuge (not shown) for centrifugation.
  • a centrifuge not shown
  • the first tube body 10 and the second tube body 20 are placed at two adjacent tube positions in the centrifuge.
  • the connecting tube 24 is partially coiled around the outlet 121 and stored in the pipe fittings of the first tube body 10. Within space 123. This can prevent the connecting tube 24 from excessive shaking during the centrifugation operation.
  • the fluid in the first tube body 10 has been separated into a supernatant (for example, plasma when whole blood is centrifuged) and a precipitate (for example, blood cells when whole blood is centrifuged).
  • a supernatant for example, plasma when whole blood is centrifuged
  • a precipitate for example, blood cells when whole blood is centrifuged.
  • An air pressure source 70 can optionally be removably connected to the air hole 231 of the second tube body 20 in order to utilize suction force to accelerate the speed of sediment movement.
  • the bottom 12 of the first pipe body 10 has a funnel shape, that is, a downward tapered structure, and the outlet 121 is located at the lowest position of the bottom 12 of the first pipe body 10, the operator can accurately control the movement of the sediment.
  • the supernatant liquid will not accidentally enter the second tube body 20 .
  • the valve 60 is used to close the connecting pipe 24, and the air hole 231 of the second pipe body 20 after the air pressure source 70 has been removed is closed again, so that the supernatant and the sediment can be separated.
  • the objects are sealed in the first tube body 10 and the second tube body 20 respectively.
  • another set of closed cell separation operating devices of the present invention can be connected in series after the first set of closed cell separation operating devices.
  • the extension tube 50 can be used to connect the first tube body 10 of the second set of closed cell separation operating devices.
  • the extension pipe 50 can be coiled around the outlet 221 and stored in the pipe storage space 223 of the second pipe body 20 before centrifugation, in the same manner as the connecting pipe 24 described above.
  • FIG. 6 depicts another embodiment of the closed cell separation operating device of the present invention.
  • the closed cell separation operation device of the present invention can also be provided with another second ventilating tube 90 attached to the air hole 231 of the second tube body 20 .
  • the second vent pipe 90 is also equipped with a valve member 60 for opening and closing it.
  • the second vent pipe 90 is connected with an additional filter 91 to prevent foreign matter from entering the second pipe body 20 and further ensure a pollution-free operating environment.
  • the air pressure source 70 as mentioned above can be connected to the air hole 131 of the first tube body 10 to push the contents of the first tube body 10 into the second tube body 20 .
  • valve components 60 may each be a pipe wrench, a flow control valve, a multi-way faucet connector or a fluid switching valve, but are not limited thereto.
  • the air pressure source 70 attached to the air hole 131 of the first pipe body 10 and/or the air hole 231 of the second pipe body 20 can be an air pump or an air injector, or anything else capable of changing the pressure in the first pipe body 10 and the second pipe body 20 A device to promote fluid flow from the first pipe body 10 to the second pipe body 20 .
  • centrifugation is widely used in various medical and biological applications and is well known to those skilled in the art, so for the sake of brevity, the relevant operations and principles will not be described in detail.
  • cell separation technology involving the use of the closed cell separation operating device of the present invention is also familiar knowledge to those skilled in the art, and therefore will not be discussed further here.
  • the closed cell separation operating device of the present invention can provide a closed and pollution-free environment for the supernatant separation operation during and after centrifugation, while avoiding the risk of splashing and eliminating the need to purchase expensive laboratory equipment.

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Abstract

一种封闭型细胞分离操作装置包括具有气孔及入口的第一管体（10）、具有气孔（131）及入口的第二管体（20）及连接第一管体（10）与第二管体（20）的连接管（24），第一管体（10）上设有可供空气进出第一管体（10）的气孔（131），第一管体（10）具有可供来自外部流体源的流体进入第一管体（10）的入口（132），且第一管体（10）具有出口（121）；第二管体（20）上设有可供空气进出第二管体（20）的气孔（231），第二管体（20）具有可供来自第一管体（10）的出口（121）的部分流体进入第二管体（20）的入口（232），且第二管体（20）具有出口（221）。所述的封闭型细胞分离操作装置为离心中及离心后上清液的分离提供封闭无污染的环境。

Description

封闭型细胞分离操作装置 技术领域
本发明属于细胞离心分离的技术领域，更具体而言是关于一种能够为离心中及离心后上清液分离提供封闭无污染操作环境的封闭型细胞分离操作装置。
 背景技术
离心是普遍用于细胞相关研究和检验的技术，目的在于分离出骨髓浓集液(BMAC)、脑脊髓液(CSF)、外周血、尿液、腹水、细胞培养基等检体中的细胞。
离心管是离心分离操作中的必要工具，其常见的形式是带盖的试管，例如以Nunc名称贩卖的离心管(美国ThermoFisherScientific公司)。根据现有技术，需要接受离心处理的流体必须倒入离心管，然后将离心管封合，置入离心机进行处理。离心处理后，若要去除分离的上清液(例如全血离心时的血浆)并取得沉淀物(例如全血离心时的血球细胞)，或相反，操作者必须开启试管，把上清液倒入一个备用容器中，如此才能将两部分分离。但这个操作无可避免地会使上清液和沉淀物暴露在周遭环境中，因而产生污染风险。为避免这个问题，一种解决方法是将整套操作移到无尘室或生物安全柜(BSC)中进行，但相关设备价格高昂且难以移动，并不适合临床用途。
 技术问题
传统离心后上清液分离方法的另一个问题是转移离心管内容物的过程中容易发生喷溅，使得可用的检体量减少。这不仅不利于之后的研究或检验，甚至可能导致研究或检验完全无法进行。
因此，在此技术领域中需要一种新颖的离心后上清液分离装置，其能够有效分离细胞，同时防止检体污染，也能避免检体喷溅风险，并且免除购置昂贵实验室设备的需要。
 技术解决方案
基于上述需求，本发明的主要目的是提供一种封闭型细胞分离操作装置，其可提供封闭的无污染环境，以便进行离心中或离心后的上清液分离，同时避免检体喷溅风险，并且免除购置昂贵实验室设备的需要。
为达成本发明的上述及其他目的，本发明所提供的封闭型细胞分离操作装置包括：一第一管体，其为具有一周壁的筒状容器，该第一管体具有封闭的顶部，经由一气孔及一入口以可封闭的方式与外界连通，其中该气孔为可供气体通过而进出该第一管体的气孔，且该入口为可供来自一外部流体源的流体进入该第一管体的入口；且该第一管体具有封闭的底部，经由一形成在中央的出口以可封闭的方式与外界连通；一第二管体，其为具有一周壁的筒状容器，该第二管体具有封闭的顶部，经由一气孔及一入口以可封闭的方式与外界连通，其中该气孔为可供气体通过而进出该第二管体的气孔，且该入口为可供来自该第一管体出口的该流体的至少一部分进入该第二管体的入口，该第二管体具有封闭的底部，经一形成在中央的出口以可封闭的方式与外界连通；及一连接管，设置在该第一管体的出口与该第二管体的入口之间，将该第一管体通过该连接管与该第二管体连接。
进一步的，该第一管体或/和第二管体的周壁朝管体的底部向下延伸，周壁与底部在管体的出口周围形成一个管件收纳空间，且至少部分的该连接管或/和至少部分的延伸管能够盘绕于该出口周围，并收纳在该管件收纳空间内。
进一步的，在所述封闭型细胞分离操作装置中，还包括一设在该第二管体出口的延伸管，该第二管体通过延伸管与又一所述封闭型细胞分离操作装置的又一所述第一管体连接。
进一步的，在所述封闭型细胞分离操作装置中，第一管体及第二管体的周壁中的至少一个下缘处形成有一供该连接管通过的槽口，因此当该第一管体/第二管体站立于平面时，该第一管体/第二管体的管件收纳空间留有一通道，可供连接管通过。
进一步的，在所述封闭型细胞分离操作装置中，所述连接管及延伸管中的至少一个配备有一阀件，用于封闭或开启该连接管或/和延伸管，且所述阀件是选自管钳、流量控制阀、多路龙头连接器及流体开关阀中的至少一个。
进一步的，在所述封闭型细胞分离操作装置中，该第一管体及第二管体中至少一个的底部具有一个向下收束的锥形结构，其直径渐缩且在该第一管体或/和第二管体的出口处达到最小。
进一步的，在所述封闭型细胞分离操作装置中，所述第一管体及第二管体的气孔中的至少一个配备有一用于过滤通过气孔的空气的过滤器。
进一步的，在所述封闭型细胞分离操作装置中，还包括一个设在该第一及第二管体中至少一个的气孔处的气压源，该气压源用于将空气推入或抽出该第一管体或/和第二管体。
所述气压源是气泵或空气喷射器。
进一步的，在所述封闭型细胞分离操作装置中，所述第一管体及第二管体的入口及气孔中的至少一个设置一以气密方式封合的密封件。
进一步的，在所述封闭型细胞分离操作装置中，所述第一管体及第二管体的入口及气孔中的至少一个还设有一条配备有阀件的导管。
所述阀件是选自管钳、流量控制阀、多路龙头连接器及流体开关阀中的至少一个。
 有益效果
本发明的有益效果为：
本发明的封闭型细胞分离操作装置，可提供封闭的无污染环境，以便进行离心中或离心后的上清液分离，同时能够避免检体喷溅风险，并且免除购置昂贵实验室设备的需要。
 附图说明
图1是本发明一种实施例中封闭型细胞分离操作装置的立体透视图；
图2至图4是本发明封闭型细胞分离操作装置的应用剖视图，图示封闭型细胞分离操作装置在离心中及离心后上清液分离的操作状态；
图5是封闭型细胞分离操作装置另一种实施方式的立体透视图，其中两套本发明封闭型细胞分离操作装置彼此串联；
图6是本发明另一种实施例中封闭型细胞分离操作装置的立体透视图；
图中，10、第一管体；11、21、开口；121、221出口；12、22、底部；122、222、周壁；123、223、管件收纳空间；124、224、槽口；13、第一帽盖；131、231、气孔；132、232、入口；20、第二管体；23、第二帽盖；24、连接管；30、透气管；31、过滤器；40、进液管；50、延伸管；60、阀件；70、气压源；80、密封件；90、第二透气管；91、额外过滤器。
 本发明的最佳实施方式
虽然以下是针对较佳实施例说明本发明的概念，但应知图中所示实施例的组件仅是以便于说明为考虑，且未必按照实际比例显示。
在本说明书中，除非上下文另有指明，否则单数型态的“一”应包括复数参照。参照图1和图2，根据本发明一种实施例，封闭型细胞分离操作装置主要包括附有第一帽盖13的第一管体10、附有第二帽盖23的第二管体20以及连接第一管体10与第二管体20的连接管24。
由于第一管体10与第二管体20在结构方面实质上相同，为求简洁，以下仅就其中一者进行描述。第一管体10或第二管体20是一个由周壁122或222围设而成的筒状容器。第一管体10或第二管体20具有经由开口11或21而对外界完全开放的顶部，且具有封闭但在中央形成有对外的出口121或221的底部12或22。具体而言，底部12或22具有漏斗状且向下成锥状的结构，其直径自上而下逐渐缩减，在出口121或221周围达到最小。第一管体10或第二管体20的周壁122或222向下延伸超过封闭的底部12或22，因而与漏斗状的底部12或22共同在出口121或221周围形成管件收纳空间123或223。周壁122或222的下缘还形成有一个槽口124或224，因此当第一管体10或第二管体20站立于平面时，便会为管件收纳空间123或223形成一个通道。其中，第一管体10或第二管体20的大小尺寸可以是与现有的离心管或采血管相似，因此可以兼容于现有的离心机。
同样地，由于第一帽盖13与第二帽盖23在结构方面实质上相同，为求简洁，以下仅就其中一者进行描述。第一帽盖13或第二帽盖23可自上而下盖合于第一管体10或第二管体20顶部，且设有气孔131或231及入口132或232。如下文将详述的，气孔131或231及入口132或232各自是配置成在使用时以可拆除的方式连接有导管且/或在不使用时以可拆除的方式受密封件80气密封合。
应知虽然在本实施例中，第一管体10或第二管体20上的帽盖13或23设有气孔131或231及入口132或232，但在其他实施例中，本发明封闭型细胞分离操作装置的气孔131或231及入口132或232可以是直接设置在第一管体10或第二管体20封闭的顶部上，而不是使用帽盖13或23来盖合第一管体10或第二管体20。
连接管24设置在第一管体10出口121与第二管体20入口232之间，使得第一管体10与第二管体20产生流体连通。连接管24可以部分盘绕于第一管体10的出口121周围，妥善收纳于第一管体10的管件收纳空间123中。由于第一管体10周壁122上形成有槽口124，连接管24可在第一管体10与第二管体20之间延伸，而不影响第一管体10稳固站立于任何平面。连接管24还设有一个用于关闭或开启连接管24的阀件60。
使用时，在连接管24被阀件60关闭的情况下，第一管体10的入口132与待处理流体来源(例如血袋、采血针或检体管，图未示)连通的进液管40连接。此时，第一管体10的气孔131保持开启，因此待处理的流体(例如全血)可以进入第一管体10，如图2所示。在此，第一管体10的气孔131可以设有过滤器31，防止异物进入第一管体10，确保无污染的操作环境。具体而言，如图2所示，设有过滤器31的透气管30与第一管体10的气孔131连接。所述透气管30同样可以受到阀件60控制而开启或关闭。待流体填充于第一管体10后，可使用密封件80直接将第一管体10的入口132气密盖合，或使用密封件80将仍连接在第一管体10的入口132处的进液管40气密盖合，如图1所示。同理，可使用密封件80直接将第一管体10的气孔131气密盖合，或使用密封件80将仍连接在气孔131处的透气管30气密盖合，如图1所示。
在此要说明的是，当连接管24、透气管30及进液管40受其各自阀件60关闭时，第一管体10的内部与外界隔开，形成一个封闭环境。同样，此时第二管体20的气孔231和出口221也都封闭，因此与外界隔离。
而后可将整套封闭型细胞分离操作装置移入离心机(图未示)进行离心处理。离心过程中，第一管体10及第二管体20放置在离心机中相邻的两个管位，此时连接管24部分盘绕在出口121周围并收纳在第一管体10的管件收纳空间123内。如此可以防止连接管24在离心动作的过程中过度晃动。
现参照图3，第一管体10中的流体此时已分离成上清液(例如全血离心时的血浆)与沉淀物(例如全血离心时的血球细胞)。随后，要将沉淀物移入第二管体20时，先将连接管24上的阀件60开启，并将第一管体10透气管30和第二管体20气孔231上的帽盖80分别取下。可随选将一气压源70以可移除的方式连接到第二管体20的气孔231上，以便利用抽吸力加快沉淀物移动的速度。
由于第一管体10的底部12具有漏斗状，也就是向下呈现锥形的结构，而且出口121是位于第一管体10底部12最低的位置，因此操作者能够精准控制沉淀物的移动，不会不慎使上清液进入第二管体20。此外，由于沉淀物是直接流入第二管体20，所以能够完全避免喷溅的风险。待沉淀物进入第二管体20后，再使用阀件60关闭连接管24，并将已经拆除气压源70后的第二管体20气孔231再次盖合，如此便可将上清液和沉淀物分别密封在第一管体10及第二管体20中。通过以上结构，可以达成沉淀物与上清液高效可靠的分离，如图4所示。
参照图5，在各种需要多重分离的应用中，可将另一套本发明封闭型细胞分离操作装置串联在第一套封闭型细胞分离操作装置之后。具体而言，可使用延伸管50连接第二套封闭型细胞分离操作装置的第一管体10。在此情况下，延伸管50可于离心之前盘绕在出口221周围并收纳在第二管体20的管件收纳空间223中，如同以上所述连接管24的收纳方式。
图6描绘本发明封闭型细胞分离操作装置的另一实施例。如图6所示，本发明封闭型细胞分离操作装置还可设有另一个附加在第二管体20气孔231处的第二透气管90。此第二透气管90同样配备有用于将之开关的阀件60。第二透气管90连接有额外过滤器91，以防止异物进入第二管体20，并进一步确保无污染的操作环境。在此情况下，如上文所述的气压源70可以连接到第一管体10的气孔131上，用以将第一管体10的内容物推入第二管体20。
上述阀件60可各自为管钳、流量控制阀、多路龙头连接器或流体开关阀，但不以此为限。
附加在第一管体10气孔131和/或第二管体20气孔231处的气压源70可以是气泵或空气喷射器，或其他任何能够改变第一管体10、第二管体20中压力以促进流体从第一管体10流向第二管体20的装置。
最后，离心广泛用于各种医疗和生物应用且为本领域技术人员所熟知，因此为求简洁，相关操作和原理不再详述。同样地，涉及使用本发明封闭型细胞分离操作装置的细胞分离技术也属于本领域技术人员熟悉的知识，因而在此不多加讨论。
通过上述配置，本发明的封闭型细胞分离操作装置能够为离心中及离心后的上清液分离操作提供封闭无污染的环境，同时避免喷溅风险，并且免除购置昂贵实验室设备的需要。
以上所述仅为本发明的较佳实施例，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包括在本发明的保护范围之内。

Claims (14)

  1. 一种封闭型细胞分离操作装置，其特征在于，包括：
    一第一管体，其为具有一周壁的筒状容器，该第一管体具有封闭的顶部，经由一气孔及一入口以可封闭的方式与外界连通，其中该气孔为可供气体通过而进出该第一管体的气孔，且该入口为可供来自一外部流体源的流体进入该第一管体的入口；且该第一管体具有封闭的底部，经由一形成在中央的出口以可封闭的方式与外界连通；
    一第二管体，其为具有一周壁的筒状容器，该第二管体具有封闭的顶部，经由一气孔及一入口以可封闭的方式与外界连通，其中该气孔为可供气体通过而进出该第二管体的气孔，且该入口为可供来自该第一管体出口的该流体的至少一部分进入该第二管体的入口，该第二管体具有封闭的底部，经一形成在中央的出口以可封闭的方式与外界连通；及
    一连接管，设置在该第一管体的出口与该第二管体的入口之间，第一管体通过该连接管与该第二管体连接。
  2. 如权利要求1所述的封闭型细胞分离操作装置，其特征在于，该第一管体的该周壁朝该第一管体的该底部向下延伸，该周壁与该底部在该第一管体的该出口周围形成一个管件收纳空间。
  3. 如权利要求2所述的封闭型细胞分离操作装置，其特征在于，该第一管体的该周壁下缘处形成有一供该连接管通过的槽口。
  4. 如权利要求1所述的封闭型细胞分离操作装置，其特征在于，还包括一设在该第二管体出口的延伸管，该第二管体通过延伸管与又一所述封闭型细胞分离操作装置的又一所述第一管体连接。
  5. 如权利要求4所述的封闭型细胞分离操作装置，其特征在于，该第二管体的该周壁朝该第二管体底部向下延伸，该周壁与该底部在该第二管体的该出口周围形成一个管件收纳空间。
  6. 如权利要求5所述的封闭型细胞分离操作装置，其特征在于，该第二管体的该周壁下缘处形成有一供该连接管通过的槽口。
  7. 如权利要求1所述的封闭型细胞分离操作装置，其特征在于，该连接管配备有一用于封闭或开启该连接管的阀件。
  8. 如权利要求4所述的封闭型细胞分离操作装置，其特征在于，该延伸管配备有一用于封闭或开启该延伸管的阀件。
  9. 如权利要求2所述的封闭型细胞分离操作装置，其特征在于，该第一管体的该底部具有一个向下收束的锥形结构，其直径渐缩且在该第一管体的该出口处达到最小。
  10. 如权利要求5所述的封闭型细胞分离操作装置，其特征在于，该第二管体的该底部具有一个向下收束的锥形结构，其直径渐缩且在该第二管体的该出口处达到最小。
  11. 如权利要求1所述的封闭型细胞分离操作装置，其特征在于，该第一管体及第二管体的该气孔中的至少一个配备有一用于过滤通过该气孔的空气的过滤器。
  12. 如权利要求1所述的封闭型细胞分离操作装置，其特征在于，该第一管体及第二管体的该气孔中的至少一个配备有一用于将空气推入或抽出该第一管体或/和第二管体的气压源。
  13. 如权利要求1所述的封闭型细胞分离操作装置，其特征在于，该第一管体及第二管体的该入口及该气孔中的至少一个设置一以气密方式封合的密封件。
  14. 如权利要求1所述的封闭型细胞分离操作装置，其特征在于，该第一管件及第二管体的该入口及该气孔中的至少一个还设有一条配备有一阀件的导管。
PCT/CN2022/082170 2022-03-22 2022-03-22 封闭型细胞分离操作装置 WO2023178498A1 (zh)

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