WO2023160434A1 - Dispositif de réfrigération et son microtome de cryostat - Google Patents

Dispositif de réfrigération et son microtome de cryostat Download PDF

Info

Publication number
WO2023160434A1
WO2023160434A1 PCT/CN2023/075886 CN2023075886W WO2023160434A1 WO 2023160434 A1 WO2023160434 A1 WO 2023160434A1 CN 2023075886 W CN2023075886 W CN 2023075886W WO 2023160434 A1 WO2023160434 A1 WO 2023160434A1
Authority
WO
WIPO (PCT)
Prior art keywords
plate
refrigeration
block
refrigeration device
board
Prior art date
Application number
PCT/CN2023/075886
Other languages
English (en)
Chinese (zh)
Inventor
黄包秀
王贤强
阳方义
陈华贵
Original Assignee
深圳市瑞沃德生命科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 深圳市瑞沃德生命科技有限公司 filed Critical 深圳市瑞沃德生命科技有限公司
Publication of WO2023160434A1 publication Critical patent/WO2023160434A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
    • F25B21/00—Machines, plants or systems, using electric or magnetic effects
    • F25B21/02—Machines, plants or systems, using electric or magnetic effects using Peltier effect; using Nernst-Ettinghausen effect
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
    • F25B49/00—Arrangement or mounting of control or safety devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
    • F25B2321/00—Details of machines, plants or systems, using electric or magnetic effects
    • F25B2321/02—Details of machines, plants or systems, using electric or magnetic effects using Peltier effects; using Nernst-Ettinghausen effects
    • F25B2321/021—Control thereof

Definitions

  • the present application relates to the technical field of cryostats, in particular to a refrigeration device and a cryostat.
  • a cryostat generally includes a cryostat cavity, which provides a cryostat environment for tissue sample sections.
  • a tissue sample, a sample head and a tool holder are accommodated in the cryostat chamber.
  • the knife holder is fixed, the tissue sample is held by the sample head, and a specific mechanism (not shown in the figure) drives the tissue sample and the sample head to make a vertical reciprocating movement (in the direction of the arrow in the figure) relative to the knife holder for sectioning.
  • the top of the low temperature and constant temperature chamber is a transparent glass window, through which the slices in the low temperature and constant temperature chamber can be observed.
  • the above-mentioned mechanism for driving the tissue sample and the sample head to perform vertical reciprocating motion not only supports the tissue sample and the sample head, but also has a cooling function, which can better maintain the tissue sample in a frozen state.
  • thermoelectric cooler (Thermo Electric Cooler, TEC) is a thermoelectric exchange device. Its basic working process is: N-type semiconductor and P-type semiconductor form a galvanic couple, and a DC current is input in the galvanic couple circuit, and the galvanic couple is Current will flow and energy transfer will occur, and heat will be released at one node, and conversely, heat will be absorbed at the other node, thus forming a cooling surface and a heat dissipation surface on both sides of the TEC.
  • the temperature regulation of the TEC can be realized by controlling the input direct current. At the same time, the thermal inertia of TEC is very small, and a large temperature difference can be achieved between the hot surface and the cold surface in a very short time.
  • TEC With its excellent performance, TEC has been widely used, not only in daily life, such as small refrigerators, air conditioners, water dispensers, etc., but also in military and medical fields, such as infrared detection of submarines and missiles, blood Analyzer etc.
  • TEC also has its own shortcomings, such as relatively low shear strength. Combined with the existing installation methods (welding, bonding, and bolting), TEC cannot play a good supporting role in the shear direction, and it is also not conducive to later maintenance.
  • the embodiment of the present application provides a refrigeration device, which aims to solve the problems in the prior art that the mechanism including the TEC cannot play a good supporting role in the shear direction and the TEC is difficult to be easily removed for maintenance.
  • a refrigeration device including:
  • Fixing means (4) connected to the first plate (1);
  • first plate (1) and the second plate (2) are locked by a controllable torque, and the first plate (1) is locked by a fixing device (4) to fix the refrigeration device.
  • a cryostat including a low-temperature constant temperature cavity, a sample head, a knife holder, and a refrigeration device as described above; the first plate (1) of the refrigeration device supports the sample head, and drives the sample head in the low-temperature constant temperature chamber.
  • the body makes a linear reciprocating motion relative to the tool holder.
  • the refrigeration block is locked between the first plate and the second plate through a controllable torque, and the first plate is fixed by a fixing device.
  • the components play a supporting role, which improves the shear strength, and the refrigeration block can be removed for maintenance by loosening the locking torque between the first plate and the second plate, without causing damage to the refrigeration block.
  • Fig. 1 is a partial schematic diagram of a frozen microtome
  • Fig. 2 is an exploded view of the refrigeration device provided in Embodiment 1 of the present application;
  • Fig. 3 is a schematic diagram of the groove provided in Embodiment 1 of the present application.
  • Fig. 4 is a schematic diagram of the installation of the refrigeration device provided in Embodiment 1 of the present application.
  • the refrigeration block is locked between the first plate and the second plate through a controllable torque, and the first plate is fixed by a fixing device.
  • the components play a supporting role, which improves the shear strength, and the refrigeration block can be removed for maintenance by loosening the locking torque between the first plate and the second plate, without causing damage to the refrigeration block.
  • Fig. 2 is an exploded view of the refrigeration device provided in Embodiment 1 of the present application. As shown in Fig. 2, the refrigeration device includes a first plate 1, a second plate 2, a refrigeration block 3 and a fixing device (4).
  • the refrigeration block 3 is located between the first plate 1 and the second plate 2, and the first plate 1 and the second plate 2 are locked by controllable torque. As shown in Figure 2, let the screw pass through the side ear of the first plate 1 and the side ear of the second plate 2, and control the locking torque of the screw to lock the refrigeration block 3 between the first plate 1 and the second plate 2, which can avoid In the process of tightening the screws, the refrigeration block 3 is unevenly stressed and its performance is affected by warping, and the refrigeration block 3 is squeezed and broken due to excessive force.
  • a heat-conducting silica gel cloth is arranged between the refrigeration block 3 and the first plate 1, and a heat-conducting silica gel cloth is arranged between the refrigeration block 3 and the second plate 2, which can effectively reduce the heat between the refrigeration block 3 and the first plate 1.
  • Resistance the thermal resistance between the cooling block 3 and the second plate 2, reduces the temperature transmission loss of the first plate 1, the cooling block 3 and the second plate 2, and improves the heat conduction efficiency.
  • the use of heat-conducting silicone cloth is more conducive to the disassembly and maintenance of the refrigeration block 3.
  • One side of the refrigeration block 3 cools and the other dissipates heat.
  • the refrigeration block 3 is a TEC
  • the first plate 1 is used to dissipate cold energy
  • the second plate 2 is used to dissipate heat.
  • the first plate 1 and the second plate 2 can also be interchanged, or the first plate 1 and the second plate 2 perform the same function (dissipate cold or dissipate heat)
  • the cooling block 3 can also be a heating block or is another heat conduction device, which is not limited here.
  • the fixing device 4 is connected with the first board 1, and the first board 1 is locked to other mechanisms through the fixing device 4, so as to fix the refrigeration device to other mechanisms.
  • the fixing device 4 may be a combination of parts such as screws, which is not limited here.
  • the second plate 2 and the refrigeration block 3 are not connected or fixed with the fixing device 4 or other mechanisms. When the first plate 1 is stressed in the shear direction, the force is transmitted from the first plate 1 to the fixing device 4 and other mechanisms, and will not be transmitted to the refrigeration block 3, which improves the shear strength of the refrigeration device.
  • the fixing device 4 includes a mounting plate 41 , a connecting piece 42 and a screw 43 , and the second plate 2 is located between the first plate 1 and the mounting plate 41 .
  • the screw 43 passes through the side ear of the first plate 1 and the connecting piece 42, tighten the screw 43, the first plate 1, the connecting piece 42 and the mounting plate 41 fit closely, and the first plate 1 and the connecting piece 42 are locked on the mounting plate 41 superior.
  • the height of the connecting piece 42 is set according to the thickness of the second plate 2 and the refrigeration block 3, so that the distance from the first plate 1 to the mounting plate 41 is greater than the distance from the first plate 1 to the second plate 2, and the second plate 2 and the mounting plate 41
  • the second board 2 is not in contact with, connected to or fixed with the mounting board 41 .
  • the refrigeration device is fixed to other mechanisms through the mounting plate 41 , and the mounting plates 41 of different shapes can adapt to the structural characteristics of different mechanisms.
  • the second plate 2 and the mounting plate 41 are suspended in the air, both of which are located on the same side of the first plate 1, and other devices are installed on the other side of the first plate 1, and the force in the shearing direction will not be transmitted to the refrigeration unit.
  • Block 3 can also reduce the cold transfer loss of the refrigeration device.
  • the material of the connecting piece 42 is selected as a thermal insulation material, which reduces the cooling capacity of the first plate 1 transmitted to the mounting plate 41 and other mechanisms connected to the mounting plate 41, and reduces the cooling capacity of the refrigeration device. transmission loss.
  • FIG. 3 is a schematic view around the groove provided in Embodiment 1 of the present application. As shown in Figure 3, the three on the left are open grooves, and the one on the far right is a closed groove. As shown by the arrow in the figure, the main movement of the refrigeration device is linear reciprocating motion in the shearing direction, the lower side limits the position of the refrigeration block 3 , and the other sides fix the refrigeration block 3 .
  • the first board 1 is surrounded by open grooves
  • the second board 2 is surrounded by open grooves
  • the combination of the two forms an open groove or a closed groove.
  • a closed groove can best limit the movement of the refrigeration block 3 .
  • the grooves around the first board 1 or the grooves around the second board 2 are U-shaped, and the grooves around the other are in-line, and the combination forms a closed groove.
  • the grooves around the first board 1 and the second board 2 are all right-angled, and combined to form a closed groove. According to the main movement direction of the refrigeration device and the actual situation of the product, the shape of the groove around the first plate 1 and the second plate 2 and the surrounding of the groove formed by the combination of the two can be flexibly set, which is not limited here.
  • the refrigeration device further includes a heating rod arranged in the first plate 1 , a temperature control switch, a temperature sensor 5 and a pipe 6 arranged in the second plate 2 .
  • the heating rod coated with thermal conductive silicone grease, temperature control switch and temperature sensor 5 into the first board 1, the temperature sensor 5 monitors the temperature of the first board 1 in real time, and the temperature control switch controls whether the heating rod is activated to cooperate with cooling Block 3 regulates the temperature.
  • the pipe 6 includes a channel arranged in the second plate 2 and an inlet and an outlet attached to the second plate 2 , and the brine can dissipate heat from the second plate 2 through the pipe 6 . Compared with natural heat dissipation and air-cooled heat dissipation, the noise of brine heat dissipation is low, and under the condition of the same area of the second plate 2 , its heat dissipation effect is better.
  • Fig. 4 is a schematic diagram of the installation of the refrigeration device provided in Embodiment 1 of the present application.
  • paste heat-conducting silica gel cloths of equal size on both sides of the refrigeration block 3 and then use screws to lock the first plate 1, the second plate 2 and the refrigeration block 3 with heat-conducting silica gel cloths on both sides through a certain torque
  • the refrigeration block is locked between the first plate and the second plate through a controllable torque, and the first plate is fixed by a fixing device.
  • the components play a supporting role, which improves the shear strength, and the refrigeration block can be removed for maintenance by loosening the locking torque between the first plate and the second plate, without causing damage to the refrigeration block.
  • the basic structure of the refrigeration device is the same as that of Embodiment 1, and the components that are the same as those of Embodiment 1 continue to use the same symbols as in Embodiment 1, including all the features described in Embodiment 1, and are not repeated here. repeat.
  • the cryostat provided in the embodiment of the present application includes a cryostat chamber, a sample head, a knife holder, and the refrigeration device described in Embodiment 1.
  • the first plate 1 of the refrigeration device supports the sample head, and drives the sample head to perform linear reciprocating motion relative to the knife holder in the low-temperature constant temperature chamber.
  • the knife holder is fixedly arranged in the cryogenic constant temperature cavity, the tissue sample is held by the sample head, and the sample head is fixed on the first plate 1 of the refrigeration device.
  • the refrigeration block 3 is locked between the first plate 1 and the second plate 2 by a controllable torsion force, and the first plate 1 is locked to the vertical reciprocating mechanism in the cryostat through a fixing device.
  • the first plate 1 of the refrigerating device drives the tissue sample and the sample head to perform vertical reciprocating motion relative to the knife holder for slicing, and the refrigerating device has both cooling and supporting functions.
  • the direction of the vertical reciprocating motion is the shearing direction of the refrigeration device and also the shearing direction of the refrigeration block 3 .
  • the refrigeration device is subjected to the force of the tissue sample and the sample head in the shearing direction.
  • the block 3 prevents the refrigerating block 3 from being stressed in the shearing direction, and improves the shearing strength of the refrigerating device.
  • the refrigeration block is locked between the first plate and the second plate through a controllable torque, and the first plate is fixed by a fixing device.
  • the components play a supporting role, which improves the shear strength, and the refrigeration block can be removed for maintenance by loosening the locking torque between the first plate and the second plate, without causing damage to the refrigeration block.

Landscapes

  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Thermal Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Dispositif de réfrigération et microtome de cryostat. Le dispositif de réfrigération comprend une première plaque (1) servant à dissiper le froid ; une seconde plaque (2) servant à dissiper la chaleur ; un bloc de réfrigération (3) situé entre la première plaque (1) et la seconde plaque (2) ; et un dispositif de fixation (4) relié à la première plaque (1), la première plaque (1) et la seconde plaque (2) étant verrouillées au moyen d'une torsion pouvant être commandée, et la première plaque (1) étant verrouillée au moyen du dispositif de fixation (4) de façon à fixer le dispositif de réfrigération. Le bloc de réfrigération (3) est verrouillé entre la première plaque (1) et la seconde plaque (2) au moyen d'une torsion pouvant être commandée, la première plaque (1) est fixée au moyen du dispositif de fixation (4), le bloc de réfrigération (3) fournit une réfrigération et, en outre, la première plaque (1) joue un rôle dans le support d'autres éléments dans une direction de cisaillement, de telle sorte que la résistance au cisaillement est améliorée et qu la torsion de verrouillage entre la première plaque (1) et la seconde plaque (2) est relâchée pour retirer le bloc de réfrigération (3) en vue d'un entretien et n'endommage pas le bloc de réfrigération (3).
PCT/CN2023/075886 2022-02-28 2023-02-14 Dispositif de réfrigération et son microtome de cryostat WO2023160434A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202210186297.XA CN114486474A (zh) 2022-02-28 2022-02-28 一种制冷装置及其冷冻切片机
CN202210186297.X 2022-02-28

Publications (1)

Publication Number Publication Date
WO2023160434A1 true WO2023160434A1 (fr) 2023-08-31

Family

ID=81483903

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2023/075886 WO2023160434A1 (fr) 2022-02-28 2023-02-14 Dispositif de réfrigération et son microtome de cryostat

Country Status (2)

Country Link
CN (1) CN114486474A (fr)
WO (1) WO2023160434A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114486474A (zh) * 2022-02-28 2022-05-13 深圳市瑞沃德生命科技有限公司 一种制冷装置及其冷冻切片机

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1531989A (fr) * 1967-06-29 1968-07-05 Tesla Np Platine refroidissante thermo-électrique
CN201464216U (zh) * 2009-04-03 2010-05-12 王文洲 一种生物组织全自动切片机
CN205093076U (zh) * 2015-11-04 2016-03-16 深圳市光峰光电技术有限公司 一种tec散热组件及投影装置
CN110837284A (zh) * 2019-10-25 2020-02-25 华为技术有限公司 热电模组、硬盘装置及电子设备
CN111413133A (zh) * 2020-04-20 2020-07-14 华中科技大学 一种冰冻切片机
CN212778024U (zh) * 2020-08-21 2021-03-23 北京友恩特科技有限公司 一种tec制冷器
CN114486474A (zh) * 2022-02-28 2022-05-13 深圳市瑞沃德生命科技有限公司 一种制冷装置及其冷冻切片机
CN217331858U (zh) * 2022-02-28 2022-08-30 深圳市瑞沃德生命科技有限公司 一种制冷装置及其冷冻切片机

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1531989A (fr) * 1967-06-29 1968-07-05 Tesla Np Platine refroidissante thermo-électrique
CN201464216U (zh) * 2009-04-03 2010-05-12 王文洲 一种生物组织全自动切片机
CN205093076U (zh) * 2015-11-04 2016-03-16 深圳市光峰光电技术有限公司 一种tec散热组件及投影装置
CN110837284A (zh) * 2019-10-25 2020-02-25 华为技术有限公司 热电模组、硬盘装置及电子设备
CN111413133A (zh) * 2020-04-20 2020-07-14 华中科技大学 一种冰冻切片机
CN212778024U (zh) * 2020-08-21 2021-03-23 北京友恩特科技有限公司 一种tec制冷器
CN114486474A (zh) * 2022-02-28 2022-05-13 深圳市瑞沃德生命科技有限公司 一种制冷装置及其冷冻切片机
CN217331858U (zh) * 2022-02-28 2022-08-30 深圳市瑞沃德生命科技有限公司 一种制冷装置及其冷冻切片机

Also Published As

Publication number Publication date
CN114486474A (zh) 2022-05-13

Similar Documents

Publication Publication Date Title
WO2023160434A1 (fr) Dispositif de réfrigération et son microtome de cryostat
CN217331858U (zh) 一种制冷装置及其冷冻切片机
CN114234532B (zh) 一种制冷装置及其冷冻切片机
CN208269475U (zh) 一种抽屉式冰箱散冷结构
CN213089964U (zh) 顶出风式空调室外机
CN112578257A (zh) 控温测试装置及测试设备
CN209656380U (zh) 一种便携式吸附管制冷采样装置
CN2480779Y (zh) 一种间冷式温差电致冷器
CN100450335C (zh) 一种制冷装置功率器件的散热冷却方法
CN101639300A (zh) 一种可间断供电的半导体制冷器
US11313625B2 (en) Intensified cassette-type heat dissipation module
CN109059418B (zh) 一种生物制品冻存和复苏装置及冻存和复苏方法
CN205690734U (zh) 一种半导体制冷保温罩
CN213841138U (zh) 空调室外机
CN113945024A (zh) 基于热电效应的半导体局部制冷设备及其制冷方法与应用
CN215337179U (zh) 一种半导体制冷装置的节能散热结构
JP2013033895A (ja) 電気式冷却ユニット
CN112594964A (zh) 一种平板套管式氮气冷却装置
CN214199272U (zh) 一种平板套管式氮气冷却装置
CN215930243U (zh) 一种用于生产硒化铅红外探测器的制冷装置
CN218239901U (zh) 一种微型低温镜面冷凝系统
CN111322779A (zh) 一种微型制冷装置
CN212746956U (zh) 基于热电效应的半导体局部制冷设备
JPH0623894Y2 (ja) 冷温蔵庫
CN217900266U (zh) 一种冷库制冷设备用辅助装置

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 23759059

Country of ref document: EP

Kind code of ref document: A1