WO2023151260A1 - 一种细胞培养瓶 - Google Patents

一种细胞培养瓶 Download PDF

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Publication number
WO2023151260A1
WO2023151260A1 PCT/CN2022/114727 CN2022114727W WO2023151260A1 WO 2023151260 A1 WO2023151260 A1 WO 2023151260A1 CN 2022114727 W CN2022114727 W CN 2022114727W WO 2023151260 A1 WO2023151260 A1 WO 2023151260A1
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cell culture
support rod
flask
bottle cap
moving
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PCT/CN2022/114727
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English (en)
French (fr)
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王仲
胡雅琦
王凯
杨智广
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辛普森国际生命科技(天津)有限公司
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Publication of WO2023151260A1 publication Critical patent/WO2023151260A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/24—Apparatus for enzymology or microbiology tube or bottle type

Definitions

  • the utility model relates to the technical field of cell culture devices, in particular to a cell culture bottle.
  • Cell culture refers to a method of simulating the internal environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable it to survive, grow, reproduce and maintain its main structure and function.
  • Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. No matter for the entire bioengineering technology or one of the biological cloning technologies, cell culture is an essential process, and cell culture itself is a large-scale cloning of cells.
  • Cell culture technology can transform a single cell into simple single cell or rarely differentiated multi-cell through mass culture, which is an essential part of cloning technology, and cell culture itself is cell cloning.
  • Cell culture technology is an important and commonly used technology in cell biology research methods.
  • the cell culture bottle used is a culture bottle placed horizontally and the bottle mouth is inclined, but when the liquid is replenished, it needs to be carried out under sterile conditions, but at this time, the bottle cap of the culture bottle needs to be removed and placed on an aseptic operating table , but because the bottle cap will touch the operating table, it will increase the probability of contamination of the bottle cap and affect the growth of cells in the cell culture bottle.
  • a cell culture bottle is provided with a fixing device for the bottle cap, one end of the fixing device for the bottle cap is fixed on the body of the cell culture bottle, and the other end of the fixing device fixes the bottle cap through a bearing.
  • the bottle cap fixing device includes a bottle cap fixing plate, a support rod and a moving device for the support rod.
  • the bottle cap fixing plate is in the shape of a vertical ring.
  • One end of the support rod is fixedly connected to the bottle cap fixed plate, and the other end of the support rod is movably connected to the mobile device for the support rod, and the end of the support rod connected with the mobile device for the support rod is provided with a limit plate and an angle limit protrusion, and the limit plate
  • the angle limiting protrusions are arc-shaped protrusions located on the front and rear end surfaces of the support rod, and the limit disk is fixed on the end surface of the support rod.
  • the supporting rod is provided with a moving slot on the moving device, and the top and bottom faces of the moving slot are provided with arc-shaped stop protrusions, and the length of the arc-shaped stop protrusions is less than the distance between the front end face and the rear end face of the move slot.
  • the limit plate is placed in the gap between the inner surface of the moving groove and the arc-shaped limit protrusion.
  • the angle limit protrusion can be clamped by two adjacent arc-shaped limit protrusions. Realize fixation, that is, the support rod realizes the fixed frame of the angle.
  • the device of the application utilizes the fixing device for the bottle cap that can move horizontally and can also be rotated and fixed to realize the suspension and fixation of the bottle cap during liquid replenishment, so as to prevent the bottle cap from being raised due to touching the operating table.
  • the probability of bacterial infection affects the culture of cells.
  • Fig. 1 is the three-dimensional structure schematic diagram of the overall device of the present utility model
  • Fig. 2 is the structural representation of the fixing device for the bottle cap of the present invention
  • Fig. 3 is the structural representation of the utility model support bar
  • Fig. 4 is a schematic cross-sectional structure diagram of the moving device for the support rod of the present invention.
  • the cell culture bottle provided by this program includes a cell culture bottle 1 and a bottle cap 2, the bottle cap 2 is connected to the cell culture bottle 1 through threads, and also includes a fixing device 3 for the bottle cap, the bottle cap
  • the fixing device 3 includes a bottle cap fixing plate 3.1, a support rod 3.2 and a moving device 3.3 for the support rod, the bottle cap fixing plate 3.1 is taken on the bottle cap 2 through a bearing fixing sleeve, and one end of the support rod 3.2 is fixedly connected to the bottle cap fixing plate 3.1 , the other end of the support rod 3.2 is connected to the mobile device 3.3 for the support rod, and the two support rods are respectively fixed on the front end surface and the rear end surface of the cell culture bottle 1 by the mobile device 3.3.
  • the support rod 3.2 is provided with a limit plate 3.2.1 and an angle limit protrusion 3.2.2, the limit plate 3.2.1 is fixed on the end face of the support rod 3.2, and the limit plate 3.2.1 is located on the mobile In the gap between the inner surface of the groove 3.3.2 and the arc-shaped limit protrusion 3.3.3, several angle limit protrusions 3.2.2 are respectively located on the left end surface and the right end surface of the support rod 3.2.
  • the angle limiting projection 3.2.2 is an arc-shaped projection.
  • the ends of the support rods 3.2 pass through the moving slots 3.3.2.
  • the angle limiting protrusion 3.2.2 is fixed on the end of the support rod 3.2.
  • the moving device 3.3 for the support rod includes a fixed column 3.3.1, a moving groove 3.3.2 and an arc-shaped limit protrusion 3.3.3, and the fixed column 3.3.1 and the moving groove 3.3.2 are placed horizontally
  • the moving slot 3.3.2 is in the shape of a cuboid, and the moving slot 3.3.2 is located on the end face of the fixed column 3.3.1, and several arc-shaped limiting protrusions 3.3.3 are located on the upper end face and the lower end face of the moving slot 3.3.2.
  • the bottle cap 2 when it is necessary to replenish liquid in the cell culture bottle 1 under a sterile environment, the bottle cap 2 is unscrewed first, and the bottle cap 2 will drive the support rod 3.2 to move to the right when the bottle cap 2 is unscrewed.
  • the support rod 3.2 is rotated upwards, at this time, the angle limiting protrusion 3.2.2 will be rotated to be fixed in the gap between the adjacent arc-shaped limiting protrusions 3.3.3,
  • the bottle cap 2 can be suspended and fixed, so that the cell culture bottle 1 can be directly replenished with liquid.
  • Culture flask The in vitro culture of cells and tissues has become an essential part of life research and practice.
  • a wide variety of cell types are cultured, from viruses to bacteria and fungi, from human cells to animal cells and plant cells. Some cells and tissues can grow in suspension, but a significant fraction of mammalian cells require surface attachment. Therefore, in addition to having good transparency, non-toxicity and sterility, the culture flasks, culture plates and culture dishes used to provide a cell culture environment in vitro also need surface modification to enable them to adhere, divide and grow.
  • the cell culture bottle mentioned in this patent is a common culture bottle that can be placed horizontally.
  • the support rod used in this program is a cylindrical support rod made of stainless steel.
  • the limit plate is a circular shape placed vertically.
  • the limit plate used in this solution is made of stainless steel, and the limit plate and the support rod are of an integrated structure.
  • the angle limiting protrusion mentioned in this solution is an arc-shaped protrusion made of plastic material.
  • the moving trough mentioned in this solution is a rectangular trough placed horizontally, one end of the support rod can move left and right in the moving trough, and the arc-shaped stop protrusion is an arc-shaped protrusion made of plastic material.
  • both the angle limiting protrusion and the arc-shaped limiting protrusion are made of plastic, the frictional force between them is relatively large, and it is easier to fix the support rod.
  • the angle limit protrusion and the arc limit protrusion can also be made of transparent glass or stainless steel, but they need to be frosted on their surfaces to ensure the friction between the two when they move.

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Abstract

一种细胞培养瓶，包括细胞培养瓶和瓶盖，所述瓶盖通过螺纹与所述细胞培养瓶连接，还包括瓶盖用固定装置，所述瓶盖用固定装置包括瓶盖固定盘、支撑杆和支撑杆用移动装置，所述瓶盖固定盘通过轴承固定套取在所述瓶盖上，所述支撑杆的一端固定连接所述瓶盖固定盘，所述支撑杆的另一端连接所述支撑杆用移动装置，两个所述支撑杆用移动装置分别固定在所述细胞培养瓶的前端面和后端面上。该装置利用可水平移动，也可旋转固定的瓶盖用固定装置实现在补液时对瓶盖的悬空固定，防止瓶盖因为碰触操作台而提高染菌的概率，影响细胞的培养。

Description

一种细胞培养瓶 技术领域
本实用新型涉及细胞培养装置技术领域，具体涉及一种细胞培养瓶。
背景技术
细胞培养是指在体外模拟体内环境(无菌、适宜温度、酸碱度和一定营养条件等)，使之生存、生长、繁殖并维持主要结构和功能的一种方法。细胞培养也叫细胞克隆技术，在生物学中的正规名词为细胞培养技术。不论对于整个生物工程技术，还是其中之一的生物克隆技术来说，细胞培养都是一个必不可少的过程，细胞培养本身就是细胞的大规模克隆。细胞培养技术可以由一个细胞经过大量培养成为简单的单细胞或极少分化的多细胞，这是克隆技术必不可少的环节，而且细胞培养本身就是细胞的克隆。细胞培养技术是细胞生物学研究方法中重要和常用技术，通过细胞培养既可以获得大量细胞，又可以借此研究细胞的信号转导、细胞的合成代谢、细胞的生长增殖等。目前，使用的细胞培养瓶是水平放置、瓶口倾斜的培养瓶，但是在补液时，需要在无菌条件下进行，但是这时需要将培养瓶的瓶盖取下放置在无菌操作台上，但是由于瓶盖会接触操作台，所以会增加瓶盖的染菌概率，影响细胞培养瓶内的细胞生长。
发明内容
鉴于现有技术中的上述缺陷或不足，期望提供一种细胞培养瓶。
根据本申请实施例提供的技术方案，一种细胞培养瓶，设置瓶盖用固定装置，瓶盖用固定装置一端固定在细胞培养瓶的瓶身上，另一端通过轴承固定瓶盖。
瓶盖用固定装置包括瓶盖固定盘、支撑杆和支撑杆用移动装置，瓶盖固定盘为竖直圆环状，瓶盖通过轴承固定套取在瓶盖固定盘的圆孔内，支撑杆的一端固定连接瓶盖固定盘，支撑杆的另一端活动式连接支撑杆用移动装置，与支撑杆用移动装置连接的支撑杆的端部设置限位盘和角度限位凸起，限位盘为竖直放置盘，角度限位凸起为位于支撑杆前端面和后端面的弧形凸起，限位盘固定在支撑杆的端面上。
支撑杆用移动装置上设置移动槽，移动槽的顶端面和底端面设置弧形限位凸起，弧形限位凸起的长度小于移动槽前端面与后端面之间的距 离。
限位盘放置在移动槽的内表面与弧形限位凸起之间的空隙内，支撑杆在转动时，角度限位凸起可通过被相邻两个弧形限位凸起夹住而实现固定，即支撑杆实现角度的定格。
综上所述，本申请的有益效果：本申请装置利用可水平移动，也可旋转固定的瓶盖用固定装置实现在补液时对瓶盖的悬空固定，防止瓶盖因为碰触操作台而提高染菌的概率，影响细胞的培养。
附图说明
通过阅读参照以下附图所作的对非限制性实施例所作的详细描述，本申请的其它特征、目的和优点将会变得更明显：
图1为本实用新型整体装置的立体结构示意图；
图2为本实用新型瓶盖用固定装置的结构示意图；
图3为本实用新型支撑杆的结构示意图；
图4为本实用新型支撑杆用移动装置的剖视结构示意图。
图中标号：细胞培养瓶－1；瓶盖－2；瓶盖用固定装置－3；瓶盖固定盘－3.1；支撑杆－3.2；限位盘－3.2.1；角度限位凸起－3.2.2；支撑杆用移动装置－3.3；固定柱－3.3.1；移动槽－3.3.2；弧形限位凸起－3.3.3。
具体实施方式
下面结合附图和实施例对本申请作进一步的详细说明。可以理解的是，此处所描述的具体实施例仅仅用于解释相关发明，而非对该发明的限定。另外还需要说明的是，为了便于描述，附图中仅示出了与发明相关的部分。
需要说明的是，在不冲突的情况下，本申请中的实施例及实施例中的特征可以相互组合。下面将参考附图并结合实施例来详细说明本申请。
结合图1、图2所示，本方案提供的细胞培养瓶，包括细胞培养瓶1和瓶盖2，瓶盖2通过螺纹与细胞培养瓶1连接，还包括瓶盖用固定装置3，瓶盖用固定装置3包括瓶盖固定盘3.1、支撑杆3.2和支撑杆用移动装置3.3，瓶盖固定盘3.1通过轴承固定套取在瓶盖2上，支撑杆3.2的一端固定连接瓶盖固定盘3.1，支撑杆3.2的另一端连接支撑杆用移动装置3.3，两个支撑杆用移动装置3.3分别固定在细胞培养瓶1的前 端面和后端面上。
结合图3所示，支撑杆3.2上设有限位盘3.2.1和角度限位凸起3.2.2，限位盘3.2.1固定在支撑杆3.2的端面上，限位盘3.2.1位于移动槽3.3.2内侧面与弧形限位凸起3.3.3之间的空隙内，若干个角度限位凸起3.2.2分别位于支撑杆3.2的左端面和右端面上。
角度限位凸起3.2.2为弧形凸起。支撑杆3.2的端部穿过移动槽3.3.2。角度限位凸起3.2.2固定在支撑杆3.2的端部上。
结合图4所示，支撑杆用移动装置3.3包括固定柱3.3.1、移动槽3.3.2和弧形限位凸起3.3.3，固定柱3.3.1和移动槽3.3.2均为水平放置的长方体形状，移动槽3.3.2位于固定柱3.3.1的端面上，若干个弧形限位凸起3.3.3位于移动槽3.3.2的上端面和下端面上。
如图1所示，当需要在无菌环境下向细胞培养瓶1内补液时，先将瓶盖2旋开，瓶盖2旋开的过程中，将会带动支撑杆3.2向右运动，当瓶盖2离开细胞培养瓶1时，将支撑杆3.2向上旋转，这时角度限位凸起3.2.2将会旋转至相邻的弧形限位凸起3.3.3之间的空隙内固定，即可实现瓶盖2悬空固定，这样就可以直接向细胞培养瓶1内补液。
培养瓶：细胞和组织的体外培养已成为生命研究和实践的必不可少内容。培养的细胞类型繁多,从病毒到细菌和真菌,从人体细胞到动物细胞和植物细胞。一些细胞和组织可在悬浮状态下生长,但相当一部分哺乳动物细胞需要表面贴壁。因此,用于提供细胞体外培养环境的培养瓶、培养板和培养皿除了具有良好的透明度和无毒、无菌外，还需经表面改性处理使之能够贴壁、分裂和生长。本专利中提到的细胞培养瓶为普通的可水平放置的培养瓶。
本方案中使用的支撑杆为不锈钢材质的圆柱形支撑杆。
限位盘为竖直放置的圆形形状，本方案中使用的限位盘为不锈钢材质，限位盘与支撑杆为一体式结构。
本方案中提到的角度限位凸起为塑胶材质的弧形凸起。
本方案中提到的移动槽为水平放置的长方形槽体，支撑杆的一端部可在移动槽内左右移动，弧形限位凸起为塑胶材质的弧形凸起。
由于角度限位凸起和弧形限位凸起均为塑胶材质，所以之间的摩擦力较大，较易固定支撑杆。
角度限位凸起和弧形限位凸起也可为透明的玻璃材质或不锈钢材质，但是需要在其表面做磨砂处理，以保证两者在相互运动时的摩擦力。
以上描述仅为本申请的较佳实施例以及对所运用技术原理等方案的说明。同时，本申请中所涉及的发明范围，并不限于上述技术特征的特定组合而成的技术方案，同时也应涵盖在不脱离所述发明构思的情况下，由上述技术特征或其等同特征进行任意组合而形成的其它技术方案。例如上述特征与本申请中公开的(但不限于)具有类似功能的技术特征进行互相替换而形成的技术方案。

Claims (6)

  1. 一种细胞培养瓶，包括细胞培养瓶(1)和瓶盖(2)，所述瓶盖(2)通过螺纹与所述细胞培养瓶(1)连接，其特征是：还包括瓶盖用固定装置(3)，
    所述瓶盖用固定装置(3)包括瓶盖固定盘(3.1)、支撑杆(3.2)和支撑杆用移动装置(3.3)，所述瓶盖固定盘(3.1)通过轴承固定套取在所述瓶盖(2)上，所述支撑杆(3.2)的一端固定连接所述瓶盖固定盘(3.1)，所述支撑杆(3.2)的另一端连接所述支撑杆用移动装置(3.3)，两个所述支撑杆用移动装置(3.3)分别固定在所述细胞培养瓶(1)的前端面和后端面上。
  2. 根据权利要求1所述的一种细胞培养瓶，其特征是：所述支撑杆(3.2)上设有限位盘(3.2.1)和角度限位凸起(3.2.2)，所述限位盘(3.2.1)固定在所述支撑杆(3.2)的端面上，所述角度限位凸起(3.2.2)固定在所述支撑杆(3.2)的端部上。
  3. 根据权利要求1所述的一种细胞培养瓶，其特征是：所述支撑杆用移动装置(3.3)包括固定柱(3.3.1)、移动槽(3.3.2)和弧形限位凸起(3.3.3)，所述固定柱(3.3.1)和所述移动槽(3.3.2)均为水平放置的长方体形状，所述移动槽(3.3.2)位于所述固定柱(3.3.1)的端面上，若干个所述弧形限位凸起(3.3.3)位于所述移动槽(3.3.2)的上端面和下端面上。
  4. 根据权利要求2、3所述的一种细胞培养瓶，其特征是：所述限位盘(3.2.1)位于所述移动槽(3.3.2)内侧面与所述弧形限位凸起(3.3.3)之间的空隙内，所述支撑杆(3.2)的端部穿过所述移动槽(3.3.2)。
  5. 根据权利要求2所述的一种细胞培养瓶，其特征是：若干个所述角度限位凸起(3.2.2)分别位于所述支撑杆(3.2)的左端面和右端面上。
  6. 根据权利要求2所述的一种细胞培养瓶，其特征是：所述角度限位凸起(3.2.2)为弧形凸起。
PCT/CN2022/114727 2022-02-11 2022-08-25 一种细胞培养瓶 WO2023151260A1 (zh)

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Publication number Priority date Publication date Assignee Title
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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5398837A (en) * 1993-02-15 1995-03-21 Degrassi; Alberto Cell culture flask and closure
JP2003012087A (ja) * 2001-07-02 2003-01-15 Seiko Corp ボトルキャップの支持装置とそのボトルキャップ支持用のアタッチメント
CN205839034U (zh) * 2016-07-27 2016-12-28 浙江译美生物科技有限公司 细胞培养瓶
CN208233725U (zh) * 2018-03-23 2018-12-14 柳州市妇幼保健院 一种优化结构的细胞培养瓶瓶盖
CN211999763U (zh) * 2020-03-12 2020-11-24 华中科技大学同济医学院附属协和医院 一种细胞培养瓶
CN212128200U (zh) * 2020-04-16 2020-12-11 福建省妇幼保健院 改进型细胞培养瓶
CN214087625U (zh) * 2020-12-26 2021-08-31 常州市和顺玻璃制品有限公司 一种nnpb食品用绿色环保超轻量化夹层玻璃瓶
CN214400550U (zh) * 2020-11-26 2021-10-15 安诺伦(武汉)生物科技有限公司 一种细胞培养支架及其装置
CN217025936U (zh) * 2022-02-11 2022-07-22 辛普森国际生命科技(天津)有限公司 一种细胞培养瓶

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5398837A (en) * 1993-02-15 1995-03-21 Degrassi; Alberto Cell culture flask and closure
JP2003012087A (ja) * 2001-07-02 2003-01-15 Seiko Corp ボトルキャップの支持装置とそのボトルキャップ支持用のアタッチメント
CN205839034U (zh) * 2016-07-27 2016-12-28 浙江译美生物科技有限公司 细胞培养瓶
CN208233725U (zh) * 2018-03-23 2018-12-14 柳州市妇幼保健院 一种优化结构的细胞培养瓶瓶盖
CN211999763U (zh) * 2020-03-12 2020-11-24 华中科技大学同济医学院附属协和医院 一种细胞培养瓶
CN212128200U (zh) * 2020-04-16 2020-12-11 福建省妇幼保健院 改进型细胞培养瓶
CN214400550U (zh) * 2020-11-26 2021-10-15 安诺伦(武汉)生物科技有限公司 一种细胞培养支架及其装置
CN214087625U (zh) * 2020-12-26 2021-08-31 常州市和顺玻璃制品有限公司 一种nnpb食品用绿色环保超轻量化夹层玻璃瓶
CN217025936U (zh) * 2022-02-11 2022-07-22 辛普森国际生命科技(天津)有限公司 一种细胞培养瓶

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