WO2023134778A1 - 分离组件、纯化设备及控制方法 - Google Patents

分离组件、纯化设备及控制方法 Download PDF

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Publication number
WO2023134778A1
WO2023134778A1 PCT/CN2023/072634 CN2023072634W WO2023134778A1 WO 2023134778 A1 WO2023134778 A1 WO 2023134778A1 CN 2023072634 W CN2023072634 W CN 2023072634W WO 2023134778 A1 WO2023134778 A1 WO 2023134778A1
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WO
WIPO (PCT)
Prior art keywords
magnetic
liquid
container
assembly
separation
Prior art date
Application number
PCT/CN2023/072634
Other languages
English (en)
French (fr)
Inventor
刘显昱
陈春花
廖元明
白涛
张昊
钱红
贺瑞娜
孟凯特
王林
张巍
Original Assignee
南京金斯瑞生物科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 南京金斯瑞生物科技有限公司 filed Critical 南京金斯瑞生物科技有限公司
Priority to CN202380017477.8A priority Critical patent/CN118541219A/zh
Publication of WO2023134778A1 publication Critical patent/WO2023134778A1/zh

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/10—Centrifuges combined with other apparatus, e.g. electrostatic separators; Sets or systems of several centrifuges

Definitions

  • This description relates to the technical field of biomacromolecule purification, in particular to a separation component, purification equipment and control method.
  • the chromatographic column-based purification equipment includes a chromatographic column, which can be used to purify the target substance in the culture medium (ie crude sample) Separation from the culture medium and used to separate the purified target from the non-purified target (or called impurities), so as to achieve the purification of the purified target.
  • both ends of the chromatographic column are provided with openings, and the inner cavity of the chromatographic column is filled with fillers, which can be used to mix or combine with the purified target.
  • the crude sample needs to be centrifuged and filtered to remove impurities such as cell debris, so as to avoid clogging of the chromatographic column.
  • a purification device which can realize the purification of a large sample volume while improving the purification speed and purification effect.
  • One of the present specifications is to provide a purification device, the purification device includes: a mixing assembly, the mixing assembly includes a shaking platform; a transfer assembly, the transfer assembly includes magnetic parts; a separation assembly, the separation assembly includes a separation container and a filter element arranged in the separation container, the filter element is provided with a filter hole, and the diameter of the filter hole is smaller than the diameter of the magnetic beads.
  • the magnetic member includes a magnetic rod, and the magnetic rod can move along the height direction of the vibration platform.
  • the transfer assembly further includes a magnetic rod casing, the magnetic rod casing is sleeved on the magnetic rod, and the magnetic rod casing can move relative to the magnetic rod along a casing direction.
  • the transfer assembly further includes a first track arranged along the height direction of the shaking platform, and the magnetic bar can slide along the first track.
  • the transfer assembly further includes a first track arranged along the height direction of the vibration platform and a second track arranged along the sheathing direction, the first track is opposite to the outer casing of the magnetic bar fixed, the magnetic bar can slide along the first track, and the magnetic bar cover can slide along the second track.
  • the purification device further includes a drip-proof flap, and the drip-proof flap is telescopically disposed on the bottom of the second track.
  • the transfer assembly further includes a third track arranged along the direction from above the shaking platform to above the separation container, and the magnetic bar can slide along the third track.
  • the purification device further includes a magnetic bead detachment assembly for detaching the magnetic beads enriched on the magnetic member from the magnetic member.
  • the magnetic bead detachment assembly includes a washing assembly; the washing assembly includes a washing nozzle, and the washing nozzle is arranged above the separation container.
  • the flushing assembly further includes a rotary table capable of rotating around the central axis of the separation container, and the flushing spray head is arranged on the rotary table.
  • a funnel is provided above the separation container; the narrow end of the funnel communicates with the upper opening of the separation container; the rotating platform can rotate around the wide end of the funnel.
  • the separation container includes a gravity column, and the upper opening of the gravity column communicates with the funnel; the separation assembly further includes a sealing plug, and the lower opening of the gravity column is sealed by the sealing plug; A puncture needle tube is provided at the inlet of the drainage pipeline communicated with the gravity column, and the puncture needle tube can pass through the sealing plug.
  • the separation assembly further includes: a support frame, the gravity column can be placed in the support frame; an adaptation structure, the adaptation structure is used to connect different gravity columns with the The support frame is connected.
  • the fitting structure includes: a buckle provided at the upper opening of the support frame for limiting the shaking of the gravity column; a pressure head provided at the lower opening of the support frame, the The indenter is provided with a liquid channel, the top of the indenter includes a first abutment surface, the upper opening of the liquid channel is set on the first abutment surface, and the lower opening of the liquid channel is sealed by the sealing plug
  • the lower open end of the gravity column includes a second abutment surface adapted to the first abutment surface, and when the first abutment surface abuts against the second abutment surface, the gravity column The lower opening of the bottom protrudes into the liquid channel.
  • the narrow mouth end of the funnel is provided with a compression flange, and when the compression flange is engaged with the buckle, the gravity column abuts against the pressure head.
  • the gravity column includes a first gravity column or a second gravity column of different specifications
  • the buckle includes a first buckle or a second buckle
  • the indenter includes a first indenter or a second indenter.
  • Two pressure heads; the first buckle and the first pressure head are adapted to the first gravity column, and the second buckle and the second pressure head are adapted to the second gravity column.
  • the rotating table is movable along the height direction of the gravity column.
  • the separation assembly further includes a first lifting track arranged along the height direction of the gravity column, and the rotary table can slide along the first lifting track.
  • the support frame is movable along the height direction of the gravity column.
  • the separation assembly further includes a second lifting track arranged along the height direction of the gravity column, and the support frame can slide along the second lifting track.
  • the support frame is movable in a direction perpendicular to the height of the gravity column.
  • the separation assembly further includes a translation track arranged along a direction perpendicular to the height of the gravity column, and the support frame can slide along the translation track.
  • the purification device further includes a first detection component; the first detection component includes a liquid level sensor, and the liquid level sensor is used to detect whether the liquid level in the separation container reaches a set value. Location.
  • the purification device further includes a first detection component; the first detection component includes a liquid level sensor, and the liquid level sensor is used to detect whether the liquid level in the separation container reaches a set value. Location.
  • the first detection component further includes a second liquid level sensor, the second liquid level sensor is arranged at a second position of the separation container, and the height of the first position is higher than that of the first position. The height of the second position.
  • the purification device further includes a second detection component for detecting the volume of the first liquid added into the separation container.
  • the purification device further includes a third detection component, the third detection component is used to detect the impurity content in the first liquid and/or the second Amount of purified target compound in liquid.
  • the third detection component includes a content detection sensor communicated with the drainage pipeline.
  • the content detection sensor includes an ultraviolet absorption detector.
  • the purification device further includes a collection assembly for collecting liquid from the separation vessel.
  • the collection assembly includes a liquid filling port communicating with the lower opening of the separation container and a collection container.
  • the collection assembly further includes a waste liquid temporary storage container.
  • the purification device further includes a fourth detection component, configured to detect whether the liquid level in the temporary waste liquid storage container and/or the collection container reaches a set position.
  • the purification device includes a fifth detection component, the fifth detection component includes a first photoelectric sensor, and the first photoelectric sensor is used to detect whether the first gravitational force is installed in the support frame. column or the second gravity column.
  • the collection assembly further includes a base and a collection container support, the collection container support is used to place the collection container, and the collection container support is installed on the base.
  • the collection container includes a first collection container or a second collection container of different specifications
  • the collection container support includes a first collection container support or a second collection container support
  • the first collection container support is used for For placing the first collection container
  • the second collection container holder is used for placing the second collection container
  • the size of the first collection container holder is the same as that of the second collection container holder.
  • the purification device further includes a sixth detection component, the sixth detection component includes a second photoelectric sensor, and the second photoelectric sensor is used to detect whether the first collector is installed on the base The container holder or the second collection container holder.
  • the collection assembly further includes a fourth track arranged along the direction from above the waste liquid temporary storage container to above the collection container, and the liquid filling port can slide along the fourth track.
  • the purification device further includes a control component; the control component is used to control the mixing component to perform a mixing operation, and control the movement of the magnetic part in the transfer component to the top of the separation container Open your mouth.
  • the mixing assembly also includes a mixing container, and the mixing container is arranged on the vibration platform;
  • the control assembly includes a driving unit and a control unit, and the driving unit and the vibration platform drive connected; the control unit is used to generate a first instruction, and the driving unit is used to drive the oscillation platform to vibrate the mixing container according to the first instruction.
  • the purification device further includes a magnetic bead detachment assembly; the driving unit is also drivingly connected to the magnetic bead detachment assembly and the magnetic member; the control unit is also used to generate a second instruction, the The driving unit is also used to drive the magnetic part to transfer the magnetic beads to the upper opening of the separation container according to the second instruction, and drive the magnetic bead detachment assembly to remove the magnetic beads from the magnetic part. break away.
  • the magnetic bead detachment assembly includes a washing assembly; the driving unit is also drivingly connected to the washing assembly; the control unit is also used to generate a third instruction, and the driving unit is also used to The third instruction drives the flushing component to add the first liquid to the separation container.
  • control unit is further configured to generate a fourth instruction
  • drive unit is further configured to drive the flushing component to add the second liquid to the separation container according to the fourth instruction
  • the purification device further includes a content detection sensor and a collection assembly, and the driving unit is also drivenly connected to the collection assembly;
  • the content detection sensor is used to The impurity content in the first liquid in the road generates a first content detection signal and/or the content of the purified target substance in the second liquid generates a second content detection signal;
  • the control unit is also used to control according to the second content detection signal
  • the driving unit drives the collection assembly to collect the second liquid from the separation container.
  • One of the present specifications is to provide a separation assembly, the separation assembly includes a separation container and a filter element arranged in the separation container, the filter element is provided with a filter hole, and the pore size of the filter hole is smaller than that of the magnetic beads. diameter.
  • both ends of the separation container are provided with openings.
  • the filter element includes a filter plate, the thickness direction of the filter plate is parallel to the height direction of the separation container, and the cross-sectional shape profile of the filter plate along the thickness direction is the same as that of the separation container.
  • the cross-sectional profile of the inner cavity along its height is matched.
  • the purification equipment includes: a mixing assembly, the mixing assembly includes a shaking platform; a transfer assembly, the transfer assembly includes magnetic parts; a separation assembly, the separation The assembly includes a separation container and a filter element arranged in the separation container, the filter element is provided with a filter hole, and the diameter of the filter hole is smaller than the diameter of the magnetic beads; the magnetic bead detachment assembly, the magnetic bead detachment assembly includes washing component; a collection component, used to collect the liquid from the separation container; the control method includes: controlling the mixing component to perform a mixing operation; controlling the magnetic part in the transfer component to transfer the magnetic beads to The upper opening of the separation container; control the magnetic bead detachment assembly to detach the magnetic beads from the magnetic member, so that the magnetic beads enter the separation container and accumulate on the filter; control the The flushing component adds the first liquid into the separation container; the flushing component is controlled to add the second liquid into the separation container;
  • the magnetic member includes a magnetic rod
  • the transfer assembly includes a first track arranged along the height direction of the shaking platform and a first track arranged along the direction from above the shaking platform to above the separation container.
  • the third track, the magnetic bar can slide along the first track and the third track; the control of the magnetic part in the transfer assembly transfers the magnetic beads to the upper opening of the separation container.
  • the method includes: controlling the magnetic bar to slide along the first track and the third track, and transferring the magnetic beads to the upper opening of the separation container.
  • controlling the magnetic bead detachment component to detach the magnetic beads from the magnetic member includes: controlling the washing component to wash the magnetic beads.
  • the purification device further includes a third detection component, and the third detection component is used to detect the impurity content in the first liquid in the drainage pipeline communicated with the separation container; Before the flushing component adds the second liquid into the separation container, it also includes: controlling the third detection component to detect the impurity content in the first liquid in the drain line connected to the separation container ; when the impurity content in the first liquid reaches a first content threshold and the detection time threshold remains unchanged, controlling the flushing component to stop adding the first liquid.
  • the third detection component is also used to detect the content of the purification target substance in the second liquid in the drainage pipeline communicated with the separation container; the control of the collection component to collect The first liquid and/or the second liquid from the separation container includes: controlling the third detection component to detect the Purify target content.
  • Fig. 1 is a schematic structural diagram of a purification device according to some embodiments of the specification.
  • Fig. 2 is a schematic structural diagram of a purification device according to some embodiments of the specification.
  • Fig. 3 is a schematic structural view of purification equipment according to other embodiments of the specification.
  • Fig. 4 is a schematic diagram of the purification device shown in Fig. 2 at another angle;
  • Fig. 5 is a schematic diagram of the purification device shown in Fig. 3 at another angle;
  • Fig. 6 is a schematic diagram of the purification device shown in Fig. 2 at another angle;
  • Fig. 7 is a schematic structural diagram of a transfer assembly according to some embodiments of the present specification.
  • Fig. 8 is a schematic structural diagram of a transfer assembly according to other embodiments of the present specification.
  • Fig. 9 is a schematic diagram of the transfer assembly shown in Fig. 8 at another angle;
  • Fig. 10 is a schematic structural view of a magnetic component located in a mixing container according to some embodiments of the present specification
  • Fig. 11 is a schematic structural view of a magnetic component located in a mixing container according to other embodiments of the present specification.
  • Fig. 12 is a structural cross-sectional view of a purification device according to some embodiments of the specification.
  • Fig. 13 is a structural cross-sectional view of purification equipment shown according to other embodiments of the specification.
  • Fig. 14 is a structural schematic diagram showing magnetic parts transferring magnetic beads to the outside of the mixing container according to some embodiments of the present specification
  • Fig. 15 is a structural schematic diagram showing magnetic components transferring magnetic beads to the outside of the mixing container according to other embodiments of the present specification.
  • Fig. 16 is a cross-sectional view of the structure of the purification device at another angle according to some embodiments of the present specification.
  • Fig. 17 is a structural cross-sectional view of a purification device shown at another angle according to other embodiments of the present specification.
  • Fig. 18 is a structural schematic diagram showing magnetic parts transferring magnetic beads to the upper opening of the separation container according to some embodiments of the present specification
  • Fig. 19 is a structural schematic diagram showing magnetic components transferring magnetic beads to the upper opening of the separation container according to other embodiments of the present specification.
  • Fig. 20 is a cross-sectional view of the structure of the purification device at another angle according to some embodiments of the present specification
  • Fig. 21 is a cross-sectional view of the structure of the purification equipment at another angle according to other embodiments of the present specification.
  • Fig. 22 is a schematic structural view showing the connection between the separation component and the magnetic bead separation component according to some embodiments of the present specification
  • Fig. 23 is a cross-sectional view of a separation assembly connected to a magnetic bead separation assembly according to some embodiments of the present specification
  • Fig. 24 is a cross-sectional view showing the connection between the separation component and the magnetic bead separation component according to other embodiments of the present specification;
  • Fig. 25 is a schematic structural view showing the connection between the separation component and the magnetic bead separation component according to some other embodiments of the present specification.
  • Fig. 26 is a schematic cross-sectional view of the structure in which the separation assembly shown in Fig. 25 is connected to the magnetic bead separation assembly;
  • Fig. 27 is a schematic diagram of pulling out the puncture needle tube from the sealing plug according to some embodiments of the present specification
  • Fig. 28 is a schematic diagram showing a gravity column taken out of a support frame according to some embodiments of the present specification.
  • Fig. 29 is a schematic structural view showing the connection between the gravity column and the support frame according to some embodiments of the present specification.
  • Fig. 30 is a schematic structural view showing the connection between the first gravity column and the second gravity column and the supporting frame according to some embodiments of the present specification
  • Fig. 31 is a schematic cross-sectional view showing the connection between the support frame and the support frame slot according to some embodiments of the present specification
  • Fig. 32 is a partially enlarged schematic diagram of A in Fig. 31;
  • Fig. 33 is a schematic structural diagram of a collection assembly according to some embodiments of the present specification.
  • Fig. 34 is a schematic diagram of the connection between different collection containers and collection container supports according to some embodiments of the present specification.
  • Fig. 35 is a schematic structural view of the collection container bracket installed on the base according to some embodiments of the present specification.
  • Fig. 36 is a schematic structural diagram of a collection assembly according to some embodiments of the present specification.
  • Fig. 37 is a schematic structural diagram of a first purification equipment group according to some embodiments of the present specification.
  • Fig. 38 is a schematic structural diagram of a second purification equipment group according to some other embodiments of the present specification.
  • Figure 39 is an exemplary flow chart of a purification method according to some embodiments of the present specification.
  • the terms “a”, “an”, “an” and/or “the” are not specific to the singular and may include the plural unless the context clearly indicates an exception.
  • the terms “comprising” and “comprising” only suggest the inclusion of clearly identified steps and elements, and these steps and elements do not constitute an exclusive list, and the method or device may also contain other steps or elements.
  • the term “based on” is “based at least in part on”.
  • the term “one embodiment” means “at least one embodiment”; the term “another embodiment” means “at least one additional embodiment”. Relevant definitions of other terms will be given in the description below.
  • Fig. 1 is a schematic structural diagram of a purification device according to some embodiments of the present specification.
  • the purification device 10 may include a mixing component 11 , a transfer component 12 and a separation component 13 .
  • the mixing component 11 can be used to mix or combine the magnetic beads with the purification target.
  • the transfer component 12 can be used to transfer the magnetic beads to a specific container, so as to separate the purification target on the magnetic beads.
  • the separation component 13 can be used to accommodate magnetic beads.
  • the mixing component 11 may include a vibration platform 111 .
  • the shaking platform 111 can be used to place a mixing container 112, and the mixing container 112 is used to accommodate magnetic beads and culture fluid.
  • the vibrating platform 111 can move in the form of reciprocating swing, rotating, etc., and drives the mixing container 112 placed on the vibrating platform 111 to move.
  • the magnetic beads and the purified target in the culture medium can be mixed or combined under the movement of the shaking platform 111 .
  • the oscillating platform 111 may include an oscillating driving component for driving the oscillating platform 111 to move.
  • an oscillatory drive assembly may include an oscillatory drive motor 113 , a crankshaft 114 and a sticky plate 115 .
  • the sticky board 115 can be used to place the mixing container 112 to prevent the mixing container 112 from shaking.
  • the oscillating drive motor 113 is in drive connection with the crankshaft 114, and the crankshaft 114 is in transmission connection with the sticky plate 115.
  • the oscillating drive motor 113 drives the crankshaft 114 to move, it can drive the sticky plate 115 to move, thereby driving the mixing container 112 to move.
  • Magnetic beads may refer to magnetically responsive nanoparticles capable of binding to biomacromolecules (eg, nucleic acids, proteins, antibodies, etc.).
  • the magnetic beads can be coated with silicon base, PS base (ie polystyrene polymer material) or agarose.
  • the magnetic core material of the magnetic beads may be Fe 3 O 4 .
  • the diameter of the magnetic beads may range from 1 nm to 500 um. In some embodiments, the diameter of the magnetic beads may range from 3nm to 400um. In some embodiments, the diameter of the magnetic beads may range from 5nm to 300um. In some embodiments, the diameter of the magnetic beads may range from 50 nm to 200 um.
  • various applications can be performed according to the combination of different functional groups.
  • Functional groups include but are not limited to protein A, protein G, Ni, GSH, oligo probes, streptavidin, antibodies, etc. single or multiple or sandwich structures.
  • magnetic beads can be applied to the purification of biomacromolecules.
  • Purification refers to the separation of the target object of purification from other impurities in a crude sample (eg, culture fluid) to achieve purification.
  • the purification target may include biomacromolecules (such as proteins, antibodies, and nucleic acids, etc.).
  • the object of purification is an antibody, it can be called the antibody of interest.
  • the object of purification when the object of purification is a cell, it may be called a cell of interest.
  • Mixing may refer to separating the purified object from the culture solution and mixing it with the magnetic beads.
  • the mixing method includes but is not limited to physical mixing (for example, allowing the purification target to enter the three-dimensional mesh aperture of the magnetic beads to retain), chemical bonding or combination through ion exchange, electrostatic adsorption, etc. .
  • the purpose of mixing the purified target substance with the magnetic beads is that after the purified target substance is combined with the magnetic beads, it is only necessary to take the magnetic beads out of the culture solution, which is equivalent to separating the purified target substance from the culture solution to achieve preliminary purification.
  • the transfer assembly 12 may include a magnetic member 121 .
  • the magnetic piece 121 has magnetism and can be used to adsorb magnetic beads mixed or combined in the culture solution, so as to separate the magnetic beads from the culture solution.
  • the magnetic member 121 can also be used to transfer the magnetic beads to the upper opening of a specific container (eg, the separation container 131 ).
  • the separation assembly 13 may include a separation container 131, and a filter element 132 is disposed in the separation container 131, and the diameter of the filter holes of the filter element 132 is smaller than the diameter of the magnetic beads.
  • the upper opening of the separation container 131 allows the magnetic beads transferred by the transfer assembly 12 to enter the inner cavity of the separation container 131, and the magnetic beads are intercepted by the filter element 132 arranged in the inner cavity, so as to achieve the purpose of accommodating the magnetic beads.
  • the purification device 10 may further include a control component 18, which may be used to control one or more components of the purification device 10 to perform corresponding functions.
  • the control assembly 18 may include a control unit 181 and a drive unit 182 .
  • the control unit 181 may generate instructions and send commands to the driving unit 182 .
  • the driving unit 182 can drive corresponding components to perform corresponding functions according to instructions. Further description of the control assembly 18 can be found elsewhere in this specification.
  • liquids such as the first liquid and the second liquid can be added to the separation container 131 in sequence.
  • the first liquid can be used to wash the magnetic beads, so as to remove impurities that are not mixed or combined with the magnetic beads, so as to realize the separation of impurities. Therefore, the step of washing the magnetic beads with the first liquid can also be referred to as washing treatment.
  • the first liquid may include a washing liquid, which includes Na 2 HPO 4 , KH 2 PO 4 , NaCl, KCl, and the like.
  • the washing liquid can be different according to the type of the purification target, for example, when the purification target is nucleic acid, the washing liquid can include tris(hydroxymethyl)aminomethane, mannitol and the like.
  • the second liquid can be used to wash the magnetic beads, so as to separate the purification targets bound to the magnetic beads from the magnetic beads. It is mixed in the second liquid and discharged from the separation container 131 to facilitate the collection of the purified target substance. Therefore, the step of washing the magnetic beads with the second liquid can be referred to as an elution process.
  • the second liquid can include an eluent. Eluents can include glycine, citrate, or sodium acetate, among others.
  • the eluent can be different according to the type of purification target, for example, when the purification target is nucleic acid, the eluent can include pure water or Tris-HCl.
  • the purification device 10 provided in this specification can use the filter element 132 arranged in the separation container 131 to separate the magnetic beads from the liquid mixed with impurities or the liquid mixed with the purification target, so that the collection of the purification target More convenient.
  • the purification device 10 does not need to use a special magnetic bead filter column, nor does it need to pre-pack the second magnetic beads in the separation container 131, but directly mixes or combines the magnetic beads with the purification target
  • the beads are transferred to a separation vessel 131 provided with a filter 132 for purification.
  • a filter membrane to filter the crude sample, which reduces the cost of consumables to a certain extent.
  • the filter element 132 can also prevent the magnetic beads from being mixed in the second liquid and flow out through the filter hole 132, which effectively improves the purification effect.
  • the purification device 10 provided in this specification does not need to use a filter membrane to centrifuge and filter the crude sample, it can avoid the loss of the purification target substance caused by centrifugation and filtration, and improve the recovery rate of the purification target substance.
  • the mixing container 112 can be any container with accommodating space.
  • the mixing container 112 may be a container suitable for vibrating and rotating.
  • the mixing container 112 may be a shaker bottle.
  • an elastic member may be provided between the mixing container 112 and the shaking platform 111 .
  • Exemplary elastic members may include springs, shrapnel, and silicone pads.
  • the volume of the mixing container 112 may be determined according to the volume of the culture medium to be contained. Exemplarily, the range of the volume of the mixing container 112 may include 300ml-300L.
  • FIG. 10 to 21 respectively show the process of transferring the magnetic beads 15 from the inside of the mixing container 112 to the upper opening of the separation container 131 by using the magnetic member 121 .
  • This process includes: extending the magnetic piece 121 into the mixing container 112, and submerging a part of the magnetic piece 121 below the liquid surface (the position shown in FIG. 10 and FIG. 13 ) to adsorb the magnetic beads 15 . Control the magnetic piece 121 to move to the outside of the mixing container 112 (the position shown in FIG. 8 and FIG. 9 ). Control the magnetic piece 121 to move to the upper opening of the separation container 131 (the position shown in FIG. 14 and FIG. 17 ).
  • the magnetic piece 121 needs to be extended into different structures (eg, the mixing vessel 112 , the funnel 134 of the separation assembly 13 , etc.), so the outer diameter of the magnetic piece 121 needs to be controlled.
  • the outer diameter of the magnetic member 121 may be smaller than the diameter of the upper opening of the separation container 131 .
  • the outer diameter of the magnetic member 121 may be smaller than the diameter of the upper opening of the mixing container 112 .
  • the outer diameter of the magnetic member 121 may be smaller than the diameter of the wide-mouthed end of the funnel 134 .
  • the magnetic member 121 may include a magnetic bar 1211 .
  • the magnetic bar 1211 can move along the height direction of the vibration platform 111 .
  • the height direction of the vibration platform 111 may also refer to the thickness direction of the vibration platform 111 .
  • the height direction of the shaking platform 111 may be parallel or approximately parallel to the direction from the opening to the bottom of the mixing container 112 .
  • the height direction of the shaking platform 111 can be indicated by the arrow Y in FIG. 4 , FIG. 12 and FIG. 16 .
  • the transfer assembly 12 may further include a first track 123 arranged along the height direction of the shaking platform 111 .
  • the magnetic bar 1211 can slide along the first track 123 .
  • the top of the magnetic bar 1211 is fixedly provided with a first sliding member adapted to the first track 123 .
  • the magnetic rod 1211 can slide along the first track 123 through the first sliding member mating with the first track 123 .
  • the moving path of the magnetic bar 1211 can be regulated, and the stability of the magnetic bar 1211 during the movement can be improved.
  • the transfer assembly 12 can also include a magnetic rod housing 1212 .
  • the magnetic rod casing 1212 can be sleeved on the magnetic rod 1211 , and the magnetic rod casing 1212 can move relative to the magnetic rod 1211 along the sleeve direction.
  • the outer shell 1212 of the magnetic rod may refer to a shell structure sheathed outside the magnetic rod 1211 .
  • one end of the magnetic rod casing 1212 has an opening, and the other end is closed.
  • the magnetic rod 1211 can be inserted into the magnetic rod housing 1212 from the open end of the magnetic rod housing 1212 and move along the sheathing direction.
  • the sleeve direction may be the length direction of the magnetic rod 1211 .
  • the sheathing direction may be the same as the height direction of the vibration platform 111 .
  • the magnetic rod outer cover 1212 that is, the magnetic rod outer cover 1212 is sleeved outside the magnetic rod 1211
  • the adsorption provided by the magnetic rod 1211 The force will cause the magnetic beads 15 to concentrate on the surface of the magnetic bar jacket 1212 .
  • the magnetic rod jacket 1212 sheathed outside the magnetic rod 1211 can protect the magnetic rod 1211 and prevent the magnetic rod 1211 from directly contacting with the culture medium to cause corrosion to the magnetic rod 1211 .
  • the magnetic rod casing 1212 can move relative to the magnetic rod 1211 along the sheathing direction, the magnetic rod casing 1212 can be separated from the magnetic rod 1211 for easy cleaning or replacement.
  • the magnetic bead 15 can be separated from the magnetic rod housing 1212 by utilizing the feature that the magnetic rod housing 1212 can move relative to the magnetic rod 1211 . This is described in more detail in other embodiments of this specification, and will not be repeated here.
  • the transfer assembly 12 has a first rail 123 arranged along the height direction of the vibration platform 111 and a second rail 124 arranged along the sleeve direction.
  • the first track 123 is relatively fixed to the magnetic bar casing 1212 .
  • the magnetic bar 1211 can slide along the first track 123
  • the magnetic bar cover 1212 can slide along the second track 124 .
  • the moving paths of the magnetic bar 1211 and the magnetic bar cover 1212 can be regulated at the same time, and the stability of the magnetic bar 1211 and the magnetic bar cover 1212 can be improved during movement.
  • the open end of the magnetic bar cover 1212 is fixedly provided with a second sliding piece adapted to the second track 124, and the second sliding piece can drive the magnetic bar cover 1212 along the second track. 124 slides.
  • the first track 123 is also provided on the side of the second sliding member away from the second track 124 , so that the first track 123 is relatively fixed to the magnetic bar casing 1212 .
  • the open end of the magnetic bar cover 1212 is fixedly provided with a second sliding piece adapted to the second track 124, and the second sliding piece can drive the magnetic bar cover 1212 along the second track. 124 slides.
  • the first track 123 is also provided on the side of the second sliding member away from the second track 124 , so that the first track 123 is relatively fixed to the magnetic bar casing 1212 .
  • the second track 124 may be provided independently of the first track 123 . As shown in FIG. 8 and FIG. 9 , the first track 124 and the second track 123 can be arranged side by side.
  • the transfer assembly 12 may further include a third rail 125 arranged along the direction from above the shaking platform 111 to above the separation container 131 .
  • the magnetic member 121 (for example, the magnetic bar 1211 ) can slide along the third track 125 .
  • the direction from above the shaking platform 111 to above the separation container 131 may refer to the direction from the top opening of the mixing container 112 fixed on the shaking platform 111 to the top opening of the separation container 131 . For convenience of description, this direction can be indicated by arrow X in FIG. 4 and FIG. 6 .
  • above the vibration platform 111 may refer to directly above or obliquely above the vibration platform 111 , for example, the positions of the magnetic bar 1211 and the magnetic bar casing 1212 in FIGS. 14 to 17 .
  • Above the separation container 131 may refer to directly above or obliquely above the separation container 131 , for example, the positions of the magnetic rod 1211 and the magnetic rod outer casing 1212 in FIG. 10 and FIG. 11 .
  • the movement path of the magnetic rod 1211 and the magnetic rod casing 1212 can be standardized during the process of moving from above the shaking platform 111 to above the separation container 131 , improving the stability during the movement.
  • the descriptions of the magnetic bar 1211 sliding along the first track 123, the magnetic bar casing 1212 sliding along the second rail 124, and the magnetic bar 1211 and the magnetic bar casing 1212 sliding along the third rail 125 are only described. For illustrative purposes, it is not intended to limit the movement of the magnetic rod 1211 and the magnetic rod casing 1212 .
  • the magnetic rod 1211 and the magnetic rod cover 1212 can be moved by manual control (that is, the operator holds it by hand).
  • the magnetic rod 1211 and the magnetic rod outer casing 1212 can be moved by a driving device, for example, a manipulator controls the magnetic rod 1211 and the magnetic rod outer casing 1212 .
  • the transfer assembly 12 of the purification device 10 may further include a drip-proof baffle 126 .
  • the anti-drip flap 126 is telescopically disposed on the bottom of the second track 125 .
  • the drip flap 126 may It is configured to be in an extended state when the magnetic part 121 is located above the plane where the anti-drip baffle 126 is located, and in a retracted state when the magnetic part 121 is located below the plane where the anti-drip baffle 126 is located. For example, when the magnetic rod 1211 and the magnetic rod outer casing 1212 move to the positions shown in FIG. 14 and FIG.
  • the anti-drip baffle 126 can protrude and be located directly under the magnetic rod outer casing 1212 .
  • the anti-drip baffle 126 can be positioned under the magnetic piece 121 to catch the residual liquid contained on the magnetic beads 15 to prevent Residual liquid drips onto other parts of the purification apparatus 10 causing contamination.
  • below the magnetic member 121 may refer to directly above or obliquely below the magnetic member 121 .
  • the purification device 10 may further include a magnetic bead detachment component 133 .
  • the magnetic bead detachment component 133 can be used to separate the magnetic beads 15 enriched on the surface of the magnetic member 121 from the magnetic member 121 , so that the magnetic beads 15 enter the separation container 131 for subsequent processing of the magnetic beads 15 .
  • the magnetic bead detachment assembly 133 can include a wash assembly 1330 .
  • the washing component 1330 can be used for washing the magnetic member 121 (for example, the magnetic bar jacket 1212 covered with the magnetic bar 1211 ) adsorbed with the magnetic beads 15 to separate the magnetic beads 15 from the magnetic member 121 .
  • irrigation assembly 1330 may include irrigation jets 1331 .
  • the rinse nozzle 1331 may be disposed above the separation container 131 .
  • rinse jet 1331 may communicate with an external pump valve assembly.
  • the pump valve assembly can be used to cause the flushing jet 1331 to spray a particular liquid (eg, a first liquid, a second liquid, etc.).
  • the rinse nozzle 1331 can be arranged at any position above the separation container 131 , as long as the rinse nozzle 1331 is positioned toward the upper opening of the separation container 131 so that the sprayed liquid can flow into the separation container 131 .
  • the flushing spray head 1331 can be arranged on the upper end of the separation container 131 , and its spout is slanted downward toward the central axis of the separation container 131 .
  • the washing spray head 1331 sprays a specific liquid to wash the magnetic beads 15 on the magnetic rod casing 1212 .
  • the magnetic beads 15 are separated from the magnetic rod casing 1212 and enter the separation container 131 under the impact of the liquid.
  • the liquid ejected from the flushing nozzle 1331 will flow into the separation container 131 to wash or elute the magnetic beads 15 accumulated on the filter element 132 .
  • the flushing assembly 1330 may include a plurality of flushing nozzles 1331 , and the plurality of flushing nozzles 1331 may be disposed above the separation container 131 at intervals to improve flushing efficiency.
  • the flushing component 1330 can be used to wash and elute the magnetic beads 15 in addition to spraying the first liquid and the second liquid, and can also be used to clean the system pipeline (for example, the drainage pipeline). 17) or magnetic beads 15 for washing.
  • the flushing nozzle 1331 can spray PBS buffer solution, pure water or sodium hydroxide solution into the separation container 131, and clean the magnetic beads 15 and the inside of the assembly (for example, the inner wall of the separation container 131, the drain line 17, etc.). cleaning.
  • the sodium hydroxide solution can remove the endotoxin in the pipeline on the one hand, avoiding the introduction of too much endotoxin in the next purification experiment, which will affect the downstream experiment results.
  • the magnetic beads 15 can be cleaned and regenerated, and the magnetic beads 15 can be used for the next purification experiment after cleaning, so as to avoid waste of the magnetic beads 15 and prevent cross-contamination.
  • the flushing component 1330 may not be necessary. Addition of specific liquids (eg, first liquid, second liquid or other types of liquids) can be accomplished through a liquid addition line.
  • the upper opening of the separation assembly 13 is provided with a liquid-feeding pipeline, and the liquid-feeding pipeline can communicate with the pump valve assembly to add specific liquid into the separation container 131 as required.
  • the liquid feeding pipeline can also be combined with the flushing assembly 1330, and the two can eject different types of liquids respectively.
  • the flushing assembly 1330 sprays out the first liquid, and the liquid feeding pipeline is used to add the second liquid.
  • the flushing assembly 1330 may further include a rotating table 1332 capable of rotating around the central axis of the separation container 131 .
  • the rinse nozzle 1331 may be disposed on the rotating platform 1332 .
  • the rotating platform 1332 can be a circular member, which can be sleeved on the outer wall of the upper opening of the separation container 131 and can rotate relative to the outer wall of the separation container 131 .
  • the magnetic beads 15 in some areas of the magnetic rod casing 1212 may not be flushed. Residue on the magnetic rod housing 1212. If the number of flushing nozzles 1331 is increased, too many flushing nozzles 1331 may result in insufficient pressure of the liquid ejected from the flushing nozzles 1331, resulting in a weaker flushing effect.
  • the rotating table 1332 drives the washing nozzle 1331 to rotate around the central axis of the separation container 131, which can increase the washing area of the washing nozzle 1331 and improve the efficiency of the separation of the magnetic beads 15. , thereby improving the purification efficiency.
  • the rotating table 1332 may not be necessary, and the rotation of the flushing spray head 1331 relative to the magnetic member 121 may be implemented in other ways.
  • the magnetic member 121 may be configured to be able to rotate along its central axis. Through the rotation of the magnetic member 121 itself, the washing area of the washing nozzle 1311 can also be increased, and the detachment of the magnetic beads 15 can be accelerated.
  • the flushing assembly 1330 may further include a rotation driving assembly that drives the rotary table 1332 to rotate around the central axis of the separation container 131 .
  • the rotary drive assembly includes a rotary motor 1351, a pulley 1352 and a belt 1353, the pulley 1352 and the rotary table 1332 are connected by a belt 1353, and the rotary motor 1351 and the pulley 1352 are driven connect.
  • the rotation drive assembly may further include a gear assembly, a screw nut assembly, and the like.
  • the washing component 1330 in addition to using the washing component 1330 to wash the magnetic beads 15 to separate the magnetic beads 15 from the magnetic member 121 (for example, the magnetic rod cover 1212 ), it can also be achieved by utilizing the characteristics of the magnetic member 121 itself.
  • the magnetic bead 15 can be detached from the magnetic member 121 under the action of gravity by reducing the adsorption force of the magnetic member 121 on the magnetic bead 15 (that is, the magnetic force).
  • the magnetic rod 1211 and the magnetic rod casing 1212 can be controlled to slide along corresponding tracks to the positions shown in FIGS. 18 to 21 . Wherein, when the magnetic rod casing 1212 slides to this position along the second track 124 , the bottom of the magnetic rod casing 1212 is located in the funnel 134 of the separation assembly 13 . The magnetic rod 1211 slides to the top along the first track 123 , and the distance between the magnetic rod 1211 and the bottom of the magnetic rod casing 1212 is the farthest.
  • the bar magnet 1211 may comprise an electromagnetic bar.
  • the electromagnetic rod is magnetic when it is powered on; when the power is turned off, the magnetic rod magnetism disappears.
  • the magnetism of the electromagnetic rod can be controlled by controlling the power on/off of the electromagnetic rod, thereby controlling the adsorption force of the magnetic member 121 on the magnetic bead 15 .
  • the power supply to the electromagnetic rod can be stopped. After the electromagnetic rod is powered off, it loses its magnetism, so that the force between the magnetic rod 1211 and the magnetic bead 15 disappears, and the magnetic bead 15 can be separated from the magnetic rod jacket 1212 under the action of gravity.
  • the washing component 1330 in the foregoing embodiments can be combined with the embodiments related to reducing the adsorption force of the magnetic member 121 on the magnetic beads 15 to speed up the separation of the magnetic beads 15 from the magnetic member 121 and improve the purification efficiency.
  • the magnetic member 121 may include a magnetic rod 1211 and a magnetic rod outer casing 1212, and the magnetic rod outer casing 1212 and the magnetic rod 1211 may slide along corresponding tracks to the positions shown in FIGS. 18 to 21 ;
  • a rinse nozzle 1331 is provided.
  • the relative movement of the magnetic bar casing 1212 and the magnetic bar 1211 can reduce the adsorption force of the magnetic member 121 on the magnetic bead 15 .
  • the washing nozzle 1331 is used to wash the magnetic beads 15, which can further accelerate the separation speed of the magnetic beads.
  • both ends of the separation container 131 are provided with openings, including an upper opening and a lower opening.
  • the filter element 132 in the separation container 131 is used to filter substances that enter from a certain opening (for example, an upper opening) of the separation container 131 and are discharged from another opening of the separation container 131 .
  • the filter element 132 may be a filter plate with filter holes opened on the filter plate.
  • the thickness direction of the filter plate is parallel or approximately parallel to the height direction of the separation container 131 .
  • the cross-sectional profile of the filter plate along its thickness direction matches the cross-sectional profile of the internal cavity of the separation container 131 along its height direction.
  • the matching mentioned here may refer to the same or approximately the same shape and similar size, so that the substance to be filtered cannot pass through the gap between the filter element 132 and the inner wall of the separation container 131 .
  • the direction of the central axis of the filter element 132 may be the same or approximately the same as the direction of the central axis of the separation container 131 .
  • the magnetic beads 15 can enter the separation container 131 from the upper opening of the separation container 131 . Since the diameter of the filter holes of the filter element 132 is smaller than the diameter of the magnetic beads 15 , the magnetic beads 15 will be trapped in the separation container 131 by the filter element 132 .
  • the pore diameter of the filter hole is greater than the diameter of substances such as biomacromolecules, so that the biomacromolecules (i.e. impurities) as the non-purified target and the biomacromolecule as the purified target are separated from the magnetic beads 15 and mixed into the
  • the specific liquid may be discharged through the lower opening of the separation container 131 through the filter hole.
  • the washing solution mixed with non-purified protein can flow out from the lower opening of the separation container 131 through the filter hole, while the magnetic beads 15 will continue to accumulate on the filter element 132 to be washed.
  • the separation container 131 may be a cylindrical container with openings at both ends, and the openings at both ends communicate with each other.
  • the filter element 132 may be disposed near the lower opening of the separation container 131 .
  • the bottom of the separation container 131 is funnel-shaped, and the diameter of the lower opening is smaller than that of the rest.
  • the filter element 132 can be arranged at the wide-mouthed end of the funnel-shaped bottom (the lower opening corresponds to the narrow-mouthed end of the funnel-shaped bottom).
  • filter element 132 may be a frit.
  • the sieve plate can be detached from the separation container 131 . In some cases, it is convenient to clean or replace the sieve plate. In other cases, the magnetic beads 15 accumulated on the sieve plate can be taken out. Exemplarily, the sieve plate can move upward relative to the separation container 131 along the height direction of the separation container 131 , and be taken out through the upper opening of the separation container 131 .
  • a funnel 134 may also be provided above the separation container 131 .
  • the funnel 134 may include a wide-mouthed end and a narrow-mouthed end, and the narrow-mouthed end of the funnel 134 may communicate with the upper opening of the separation container 131 .
  • the wide-mouthed end of the funnel 134 can be used to accommodate the magnetic piece 121 (for example, the magnetic rod casing 1212), and the narrow-mouthed end can be used to communicate with the separation container 131 to ensure that the magnetic The beads 15 can smoothly fall into the separation container 131 after detaching from the magnetic member 121 .
  • the funnel 134 can prevent the residual culture solution on the magnetic member 121 or the magnetic beads 15 from dripping out of the separation container 131 to cause pollution.
  • the outer diameter of the narrow end of the funnel 134 can be smaller than the inner diameter of the separation container 131, so that the narrow end of the funnel 134 can be embedded in the upper opening of the separation container 131 to ensure that the magnetic beads 15 and/or Liquid can flow into the separation container 131 .
  • the inner diameter of the wide-mouthed end of the funnel 134 is larger than the outer diameter of the magnetic piece 121 , so that the magnetic piece 121 adsorbed with the magnetic beads 15 can smoothly extend into the funnel 134 from the wide-mouthed end.
  • the funnel 134 can be combined with the flush assembly 1330 of the previous embodiments.
  • the wide-mouthed end of the funnel 134 may be provided with a flushing nozzle 1331 .
  • the rotary table 1332 may be disposed at the wide end of the funnel 134 and may rotate along the wide end of the funnel 134 .
  • the rotary table 1332 may be disposed at the wide-mouthed end of the funnel 134 and may rotate along the wide-mouthed end of the funnel 134 , and a flushing nozzle 1331 is also provided on the rotary table 1332 .
  • the separation container 131 may include a gravity column 1311 , and the upper opening of the gravity column 1311 may communicate with the funnel 134 .
  • the separation assembly 13 may include a sealing plug 1361 through which the lower opening of the gravity column 1311 may be sealed.
  • the inlet of the drainage pipeline (for example, the drainage pipeline 17 in FIG. 21 ) communicating with the gravity column 1311 is provided with a puncture needle tube 1362 , and the puncture needle tube 1362 can pass through the sealing plug 1361 .
  • the sealing plug 1361 can be made of plastic, rubber, silica gel and other materials, so that the puncture needle tube 1362 can penetrate smoothly, and after the puncture needle tube 1362 is pulled out, the deformation can be recovered in time, so that the weight of the gravity column 1311 The lower opening is sealed.
  • a plug plate can be provided in the gravity column 1311 , and the plug plate can be used as the filter element 132 to trap the magnetic beads in the gravity column 1311 .
  • the separation assembly 13 can also include: a support frame 1363, in which the gravity column 1311 can be placed; catch.
  • different gravity columns 1311 may refer to gravity columns 1311 of different specifications.
  • the specifications of the gravity column 1311 may refer to the size parameters of the gravity column 1311 , for example, the diameter and volume of the gravity column 1311 .
  • different gravitational columns 1311 can be matched with the support frame 1363 by setting the matching structure. Therefore, even if the specifications of the gravity column 1311 are different, the gravity column 1311 and the support frame 1363 can be matched with the matching structure of the corresponding specification, so that the support frame 1363 can be compatible with different gravity columns 1311 .
  • the support frame 1363 can be a hollow columnar structure, and the gravity column 1311 can be placed inside the support frame 1363 .
  • the support frame 1363 may be provided with an observation window 13631 to facilitate the operator to observe the situation of the gravity column 1311 from the outside.
  • the fitting structure may include a buckle 1364 , and the buckle 1364 may be provided at the upper opening of the support frame 1363 to limit the gravity column 1311 from shaking.
  • the buckle 1364 can be a ring structure, for example, a snap ring.
  • the snap ring can be arranged at the upper opening of the support frame 1363, and the gravity column 1311 can be put into the support frame 1363 via the snap ring.
  • the snap ring can limit the gravity column 1311.
  • the snap ring can be interference-fitted with the gravity column 1311 so as to completely limit the shaking of the gravity column 1311 .
  • the fitting structure can also include a pressure head 1365, which can be set at the lower opening of the support frame 1363, the pressure head 1365 is provided with a liquid channel 13651, and the top of the pressure head 1365 includes a first abutting surface 13652
  • the upper opening of the liquid passage 13651 is set on the first abutment surface 13652, and the lower opening of the liquid passage 13651 is sealed by the sealing plug 1361;
  • the lower opening of the gravity column 1311 protrudes into the liquid channel 13651 .
  • the gravity column 1311 after the gravity column 1311 is put into the support frame 1363, under its own gravity, the gravity column 1311 will abut against the pressure head 1365, so that the first abutting surface 13652 and the second abutting surface 1312 The abutment allows the lower opening of the gravity column 1311 to extend into the liquid channel 13651 , and the sealed connection with the drainage pipeline can be realized through the sealing plug 1361 and the puncture needle tube 1362 .
  • the first abutting surface 13652 and the second abutting surface 1312 are matched, when the two abut, a sealing effect can be formed to prevent liquid leakage.
  • the first abutment surface 13652 and the second abutment surface 1312 may include mating tapered surfaces.
  • the second abutment surface 1312 can be an outer conical surface, that is, the conical surface protrudes outward, the lower opening of the gravity column 1311 protrudes from the bottom of the outer conical surface, and the first abutment surface
  • the junction surface 13652 can be an inner conical surface, that is, the conical surface is concave inward, and the liquid channel 13651 communicates with the bottom of the inner conical surface.
  • the first abutting surface 13652 may be an outer conical surface
  • the second abutting surface 1312 may be an inner conical surface.
  • the first abutting surface 13652 and the second abutting surface 1312 may be in the shape of a plane, a wavy surface, or the like.
  • the matching structure and the support frame 1363 may be detachably connected.
  • the buckle 1364 can be locked on the upper edge of the support frame 1363 .
  • the pressure head 1365 may be detachably connected to the lower edge of the support frame 1363 through magnetic connection, screw connection and the like.
  • the separation assembly 13 may further include a support frame slot 1366 , and the support frame slot 1366 may be used for placing the support frame 1363 .
  • the support frame slot 1366 There is an opening above the support frame slot 1366, and the support frame 1363 can be drawn from the support frame slot The upper opening of 1366 is put into the support bracket slot 1366 .
  • an O-ring 1367 can be provided at the narrow end of the funnel 134, and the O-ring 1367 is adapted to the upper opening of the gravity column 1311, and the narrow end of the funnel 134 can pass through the O-ring 1367 is in sealing connection with gravity column 1311.
  • the O-ring 1367 can be arranged on the outer edge of the narrow end, when the narrow end of the funnel 134 extends into the upper opening of the gravity column 1311, the O-ring 1367 can be connected with the gravity column 1311
  • the inner wall of the upper opening forms an interference fit, thereby ensuring the tightness of the connection between the funnel 134 and the gravity column 1311 .
  • the gravity column 1311 may include a first gravity column 1313 or a second gravity column 1314 of different specifications
  • the buckle 1364 may include a first buckle 13641 or a second buckle 13642
  • the head 1365 can include a first pressure head 13653 or a second pressure head 13654; the first buckle 13641 and the first pressure head 13643 can be adapted to the first gravity column 1313, and the second buckle 13642 and the second pressure head 13654 can be matched with The second gravity column 1314 fits.
  • the first buckle 13641 and the second buckle 13642 can be different.
  • the specifications of the first buckle 13641 and the second buckle 13642 are different.
  • the first indenter 13653 and the second indenter 13654 can be different.
  • the specifications of the first indenter 13653 and the second indenter 13654 are different.
  • FIG. 30 exemplarily shows a schematic diagram of connecting the support frame 1363 with the first gravity column 1313 and the second gravity column 1314 respectively. Wherein, since the diameter and volume of the first gravity column 1313 in Fig.
  • the diameter of the second abutting surface 1312 of the first gravity column 1313 is greater than the diameter of the second abutting surface 1312 of the second gravity column 1314, so a larger diameter of the first abutting surface 13652 is required (compared with the second pressure head 13654 first abutting surface 13652), so the diameter of the first pressure head 13653 (compared with the second pressure head 13654) is larger.
  • the buckle 1364 and the pressure head 1365 that are compatible with the gravity column 1311 of this specification can be used to use the gravity column 1311 for The support frame 1363 is connected.
  • the support frame 1363 can be compatible with the gravity column 1311 of different specifications by providing the buckle 1364 and the pressure head 1365 (that is, the matching structure) adapted to the specifications of the gravity column 1311 , thereby reducing the cost of use.
  • the support frame slot 1366 can also be continuously used, further reducing the cost of consumables.
  • the examples about the first gravity column 1313 and the second gravity column 1314 are only for illustration purposes, and do not limit the specifications of the gravity column 1311 to two types, except for the first gravity column 1313 and the second gravity column 1314,
  • the third gravity column and the fourth gravity column of other specifications may also be included.
  • the gravity column 1311 of each specification is equipped with an adapted buckle 1364 and a pressure head 1365 (ie, an adaptation structure), so as to connect the gravity column 1311 of different specifications to the supporting frame 1363 .
  • the narrow end of the funnel 134 is provided with a compression flange 1368 , and when the compression flange 1368 is matched with the buckle 1364 , the gravity column 1311 can be compressed against the pressure head 1365 superior.
  • the gravity column 1311 after the gravity column 1311 is put into the support frame 1363, it can be matched with the buckle 1364 by rotating the compression flange 1368, so as to realize the locking of the gravity column 1311 and the support frame 13631, and at the same time The gravity 1311 column is pressed against the pressure head 1365.
  • the narrow end of the funnel 134 can be provided with a magnetic attraction
  • the upper opening of the support frame 1363 can be provided with a magnet
  • the gravity column 1311 can be locked with the support frame 1363 through the magnetic attraction of the magnetic attraction and the magnet. tight.
  • the funnel 134 may include a first funnel 1341 or a second funnel 1342
  • the O-ring 1367 may include a first O-ring 13671 or a second O-ring 13672.
  • a funnel 1341 and the first O-ring 13671 can be adapted to the first gravity column 1313
  • a second funnel 1342 and the second O-ring 13672 can be adapted to the second gravity column 1314 .
  • the rotating table 1332 can move along the height direction of the gravity column 1311 so as to replace, disassemble or install the gravity column 1311 .
  • the funnel 134 needs to cooperate with the rotary table 1332 to receive the magnetic beads (as described in Figure 24 and its embodiment), and when the gravity column 1311 needs to be replaced or disassembled, the funnel 134 needs to be It is replaced and disassembled together with the gravity column 1311, but the rotating table 1332 will interfere with the funnel 134, making it inconvenient to take out the gravity column 1311, so it is necessary to control the lifting of the rotating table 1332.
  • the separation assembly 13 can also include a first lifting track 1371 arranged along the height direction of the gravity column 1311 , and the rotary table 1332 can slide along the first lifting track 1371 .
  • the separation assembly 13 may include a first lifting track 1371, a first lifting motor 1372 and a first lifting screw 1373, the first lifting motor 1372 is in transmission connection with the first lifting screw 1373, and the first lifting screw 1373 is connected with the first lifting screw 1373.
  • Rotary table 1332 is connected, the first lifting motor 1372
  • the first lifting screw 1373 can be driven to move along the first lifting track 1371 , thereby driving the rotating table 1332 to move.
  • the movement path of the turntable 1332 can be regulated, and the stability of the turntable 1332 during movement can be improved.
  • the support frame 1363 can move along the height direction of the gravity column 1311 .
  • the puncture needle tube 1362 can be controlled to pull out from the sealing plug 1361 or penetrate into the sealing plug 1361 , so as to replace, disassemble or install the gravity column 1311 .
  • the puncture needle tube 1362 needs to penetrate the sealing plug 1361 to connect the drain line with the gravity column 1311 .
  • the support frame 1363 can be controlled to move along the height direction of the gravity column 1311, so that the puncture needle tube 1362 can be pulled out from the sealing plug 1361, so that the gravity column 1311
  • the introducer needle tube 1362 is out of the way during the subsequent movement.
  • the separation assembly 13 may further include a second lifting track arranged along the height direction of the gravity column 1311, and the support frame 1363 can slide along the second lifting track.
  • the separation assembly 13 may include a second lifting motor 1381
  • the second lifting track may include a guide shaft 13661 arranged along the height direction of the gravity column 1311 of the support frame slot 1366
  • the support frame 1363 can include a guide bar 13632 arranged along the height direction of the gravity column 1311, the guide bar 13632 is adapted to the guide shaft 13661, and the second lifting motor 1381 can drive the guide bar 13632 to move along the guide shaft 13661, thereby driving the support frame 1363 to lift or drop.
  • the separation assembly 13 may include a second lift screw connected to the second lift motor 1381 in transmission, the second lift screw may be connected to the guide bar 13632, and the second drive motor may drive the second lift screw Drive the guide bar 13632 to move along the guide shaft 13661.
  • the support frame 1363 may be along a height direction perpendicular to the gravity column 1311 . After the puncture needle tube 1362 is pulled out from the sealing plug 1361 , the support frame 1363 can be controlled to move from the bottom of the rotating platform 1332 to other positions, so as to replace or disassemble the gravity column 1311 .
  • the height direction perpendicular to the gravity column 1311 may refer to a certain direction in a plane perpendicular to the height direction of the gravity column 1311 .
  • the separation assembly 13 may further include a translation track 1391 arranged along a direction perpendicular to the height of the gravity column 1311 , and the support frame 1363 can slide along the translation track 1391 .
  • the separation assembly 13 may include a translation track 1391, a translation motor 1392 and a translation screw 1393, the translation screw 1393 may be adapted to the translation track 1391, and connected to the support bracket slot 1366, the translation motor 1392 may drive the translation wire The bar 1393 slides along the translation track 1391 , thereby driving the support frame slot 1366 and the support frame 1363 disposed on the support frame slot 1366 to slide.
  • the collection assembly 14 may include a liquid filling port 141 and a collection container 145 .
  • the collection container 145 can be used to collect the second liquid mixed with the purification target (the content of the purification target reaches the second content threshold).
  • the liquid filling port 141 can communicate with the lower opening of the separation container 131 to discharge the liquid in the separation container 131 into the collection container 145 .
  • the liquid filling port 141 may communicate with the lower opening of the separation container 131 through the liquid discharge pipeline 17 of the pump valve assembly.
  • the collection assembly 14 may further include a waste liquid temporary storage container 146 .
  • the waste liquid temporary storage container 146 can be used to collect the first liquid (such as PBS washing solution) mixed with impurities in the washing process, and the first liquid mixed with the purified target substance in the elution process but whose content does not reach the second content threshold.
  • Two liquids such as glycine eluent.
  • the liquid filling port 141 may be located at the upper opening of the waste liquid temporary storage container 146 (as shown in FIG. 33 ), for draining the cleaning liquid in the separation container 131. into the waste liquid temporary storage container 146.
  • the liquid filling port 141 can still be located at the upper opening of the waste liquid temporary storage container 146, and is used to discharge the eluent. into the waste liquid temporary storage container 146. Until the content of the purification target in the eluate reaches the second content threshold, the liquid filling port 141 can be moved to the upper opening of the collection container 145 for storing the eluate in the collection container 145 .
  • the inner diameter of the temporary waste liquid storage container 146 may be larger than the inner diameter of the collection container 145 .
  • the waste liquid temporary storage container 146 not only needs to store the cleaning liquid, but may also be used to store a part of the eluent.
  • the eluate may be discharged into the waste liquid temporary storage container 146 .
  • the volume of the waste liquid temporary storage container 146 may be greater than the volume of the collection container 145 .
  • the bottom of the waste liquid temporary storage container 146 may be provided with a discharge pipeline, and the discharge pipeline may communicate with an external container with a larger volume.
  • the waste liquid in the waste liquid temporary storage container 146 can be discharged into the container through a discharge pipeline, so as to prevent the liquid in the waste liquid temporary storage container 146 from overflowing.
  • collection assembly 14 may also include an additional fill port 142 .
  • the additional liquid filling port 142 can be used to discharge other types of liquids.
  • other types of liquids can include but not limited to neutralizing liquid, pure water, sodium hydroxide solution and the like.
  • the neutralizing solution may include a buffer with a higher pH.
  • the neutralizing solution can be used to neutralize the acidic environment in the eluate in the collection container 145 to neutral pH, avoiding the Antibodies in the container 145 are destroyed in an acidic environment for a long time.
  • the additional liquid filling port 142 may not be necessary, and the neutralizing liquid may be discharged through other channels.
  • the liquid filling port 141 can be used to discharge the liquid in the separation container 131 or the neutralizing liquid.
  • the collection assembly 14 may further include a fourth track 144 arranged along the direction from above the temporary waste liquid storage container 146 to above the collection container 145 .
  • the liquid filling port 141 can slide along the fourth track 144 .
  • the moving path of the liquid filling port 141 can be regulated, the stability of the liquid filling port 141 can be improved, and the liquid discharged from the liquid filling port 141 can be guaranteed to enter the collection container. 145 or waste liquid temporary storage container 146.
  • collection assembly 14 may also include a base 147 .
  • a plurality of holes are provided along the length direction of the base 147 .
  • a plurality of collection containers 145 may be placed in corresponding holes for fixing, so as to facilitate batch operation of the plurality of collection containers 145 .
  • the direction of the fourth track 144 can be the same or approximately the same as the direction from the upper opening of one of the collection containers 145 of the base 147 to the upper opening of the other collection container 145, so that the liquid filling port 141 can be moved along the fourth rail. The track 144 moves from the upper opening of one of the collecting containers 145 to the upper opening of the other collecting container 145 .
  • the liquid filling port 141 can move to the adjacent collecting container 145 along the fourth track 144.
  • the upper opening drains liquid.
  • the waste liquid temporary storage container 146 may be disposed adjacent to the base 147 to reduce the distance required for the liquid filling port 141 to move from the waste liquid temporary storage container 146 to the collection container 145 .
  • the collection assembly 14 can include a base 147 and a collection container support 148 , the collection container support 148 can be used to place the collection container 145 , and the collection container support 148 can be installed on the base 147 .
  • the collection container holder 148 may include a plurality of placement holes, and each placement hole may place a collection container 145 for batch operation.
  • the collection container 145 can include a first collection container 1451 or a second collection container 1452 of different specifications, and the collection container holder 148 can include a first collection container holder 1481 or a second collection container holder 1481.
  • Collection container support 1482, the first collection container support 1481 can be used for placing the first collection container 1451, and the second collection container support 1482 can be used for placing the second collection container 1452, the size of the first collection container support 1481 and the second collection container Bracket 1482 is the same size.
  • the size of the first collection container support 1481 is the same as that of the second collection container support 1482 may mean that the length, height, width and other dimensions of the first collection container support 1481 are the same as the second collection container support 1482 . Since the first collection container support 1481 and the second collection container support 1482 have the same size, the length direction of the first collection container support 1481 and the second collection container support 1482 can be in the same direction, represented by the arrow X1 shown in FIG. 34 . The height direction of the first collection container support 1481 and the height direction of the second collection container support 1482 may be the same direction, represented by the arrow Y1 shown in FIG. 34 .
  • one or more first collection container holders 1481 or second collection container holders 1482 may be installed on the base 147 .
  • one or more collection containers 145 of the same specification can be placed on the collection container support 148 .
  • the volume of the first collection container 1451 is 50 ml
  • the volume of the second collection container 1452 is 125 ml
  • the outer diameter of the first collection container 1451 is smaller than that of the second collection container 1452 .
  • the first collection container support 1481 includes a plurality of first placement holes
  • the second collection container support 1482 may include a plurality of second placement holes
  • the first placement holes are adapted to the first collection container 1451
  • the second placement holes Compatible with the second collection container 1452.
  • collection container brackets 148 of the same size can be compatible with collection containers 145 of different specifications, so that they can be installed on the same base 147 , which not only facilitates batch operation, but also effectively saves equipment costs.
  • the collection assembly 14 may further include a liquid filling head 143 connected to the liquid filling port 141 .
  • the liquid filling head 143 can be used to fix the liquid filling port 141 to improve the stability of the liquid filling port 141 during operation.
  • the liquid filling head 143 may include a first board body 1431 , a second board body 1432 and a third board body 1433 fixedly connected in sequence. Wherein, the first plate body 1431 is slidably connected with the fourth track 144 .
  • the second board 1432 is arranged at a certain angle (for example, 90 degrees) relative to the first board 1431 .
  • the third board 1433 is parallel or approximately parallel to the first board 1431 (for example, the angle between the two boards is less than 5 degrees).
  • the liquid filling port 141 is disposed on the third plate body 1433 . In this embodiment, when the liquid filling head 143 composed of the first plate body 1431, the second plate body 1432 and the third plate body 1433 slides along the fourth track 144, it can drive the The liquid filling port 141 slides.
  • the detection component may include a first detection component 161 .
  • the first detection component 161 may refer to an instrument or device that detects whether the liquid level (ie, the liquid level) in the container reaches a set liquid level.
  • the first detection component 161 may include a liquid level sensor for detecting whether the liquid level in the separation container 131 reaches a set liquid level.
  • the liquid level position may refer to the position of the highest point of the liquid in the separation container 131 . According to the descriptions in other embodiments of this specification, during the purification process, it is necessary to add the first liquid to the separation container 131 to wash the magnetic beads 15, and add the second liquid to elute the magnetic beads 15 after the washing is completed.
  • the volume of liquid in the separation container 131 can be determined by the volume of liquid discharged from the flushing assembly 1330 (such as the flushing nozzle 1331 ) and the volume of liquid discharged from the separation container 131 .
  • the liquid volume in the separation container 131 may be equal to the difference between the liquid volume discharged from the flushing component 1330 and the liquid volume discharged from the separation container 131 .
  • the first detection component 161 may include a first liquid level sensor 1611 .
  • the first liquid level sensor 1611 may be disposed at the first position of the separation container 131 for detecting the relationship between the liquid level position and the first position.
  • the first position may be any position between the top (ie, the upper opening of the separation container 131 ) and the bottom (eg, where the upper surface of the filter element 132 is located) of the inner cavity of the separation container 131 .
  • the first position may be 1/3 of the height of the inner cavity of the separation container 131 .
  • the first detection component 161 may further include a second liquid level sensor 1612 .
  • the second liquid level sensor 1612 may be disposed at a second position of the separation container 131 .
  • the height of the first location may be higher than the height of the second location.
  • the first liquid level sensor 1611 can detect whether the liquid level reaches the first position, and the second liquid level sensor 1612 can detect whether the liquid level reaches the second position.
  • the first liquid level sensor 1611 arranged at the first position (for example, the first position is 4/5 of the height of the inner cavity of the separation container 131) can be used to detect the highest liquid level position (or called first level threshold). When the liquid level reaches the first position, it indicates that there is too much liquid in the separation container 131 .
  • the second liquid level sensor 1612 arranged at the second position can be used to detect the lowest liquid level position (or called the second liquid level threshold). When the liquid level does not reach the second position, it indicates that there is too little liquid in the separation container 131 .
  • the first liquid level sensor 1611 and the second liquid level sensor 1612 may be the same liquid level sensor, or may be different liquid level sensors.
  • the type of liquid level sensor may include but not limited to flange differential pressure liquid level sensor, float liquid level sensor, magnetic liquid level sensor, submersible liquid level sensor, capacitive liquid level sensor, electric float ball level sensor etc.
  • the liquid level sensor may be a capacitive liquid level sensor, so as to detect the liquid level in the separation container 131 outside the separation container 131 .
  • the detection component of the purification device 10 may further include a second detection component 162 .
  • the second detection component 162 can be used to detect the volume of the first liquid added into the separation container 131 .
  • the magnetic beads before separating the magnetic beads from the purification target, it is necessary to detect whether the impurities are completely separated from the magnetic beads. After it is determined that the impurities on the magnetic beads have been removed, the magnetic beads can be washed with the second liquid to ensure that no residual impurities on the magnetic beads are mixed in the second liquid or that the content of impurities is negligible.
  • the second detection component 162 may include a first flow sensor, and the first flow sensor may detect the volume of the first liquid added into the separation container 131 .
  • the first flow sensor can be a first flow valve, and the first flow valve can be set on the flushing spray head 1331 of the flushing assembly (such as the flushing assembly 1330 ) to detect the first flow rate of the flushing spray head 1331 . flow of liquid. When the volume of the ejected first liquid reaches a set volume threshold (which may be referred to as the first volume threshold), it indicates that the cleaning process is completed.
  • the second detection component 162 can detect the volume of the first liquid discharged through the filter hole of the filter element 132 through the lower opening of the separation container 131 .
  • the volume of the first liquid reaches the second volume threshold, it may be indicated that washing has been completed.
  • the second volume threshold may be equal to the first volume threshold.
  • the volume of the first liquid discharged from the lower opening of the separation container 131 can also be detected by the second detection component.
  • the second detection component 162 may include a second flow sensor, and the second flow sensor may detect the volume of the first liquid discharged from the lower opening of the separation container 131 .
  • the second flow sensor can be a second flow valve, and the second flow valve can be set on the discharge pipeline 17 communicating with the lower opening of the separation container 131 to detect the flow rate from the bottom of the separation container 131. The flow rate of the first liquid discharged from the opening.
  • the detection component may further include a third detection component 163 .
  • the third detection component 163 may refer to an instrument or device for detecting the content of a certain substance in the liquid.
  • the third detection component 163 can be used to detect the impurity content of the first liquid and/or the purification target content of the second liquid in the drainage pipeline 17 communicated with the separation container 131 .
  • the first liquid is added to remove the impurities on the magnetic beads 15, and the impurities on the magnetic beads 15 will be mixed in the first liquid to pass through the filter 132 The lower opening of the separation container 131 is discharged.
  • the impurities on the magnetic beads 15 will become less and less, and the content of impurities mixed in the first liquid will gradually decrease and tend to be stable.
  • the threshold can be called a baseline
  • the detection time threshold remains unchanged (that is, there is no change or almost no change)
  • a second liquid is added to separate the purified target substance (eg, target protein) from the magnetic beads 15 , and the purified target substance will be mixed in the second liquid.
  • the content of the purification target in the eluent i.e. the second liquid
  • the second liquid can be collected, so that It is collected into collection container 145 . It can be understood that as the elution continues, the content of the purified target substance in the second liquid will gradually rise to the highest point and then decrease.
  • the third content threshold may refer to the lowest value of the content of the purification target substance in the second liquid meeting the collection requirement.
  • the third detection component 163 may include a content detection sensor communicated with the liquid discharge pipeline 17 .
  • the type of content detection sensor is related to the type of impurity to be detected or the type of purified target.
  • the content detection sensor may include an ultraviolet absorption detector. The ultraviolet absorption detector can detect the content of specific substances in the liquid according to the principle that the intensity of ultraviolet light absorption of the substance to be detected is proportional to the concentration of the substance to be detected.
  • the ultraviolet absorption detector can detect the ultraviolet absorption value (or optical density value) of the first liquid at the ultraviolet absorption peak of 280nm, and then determine the content of the non-purified protein in the liquid according to the ultraviolet absorption value.
  • the ultraviolet absorption detector can detect the ultraviolet light absorption value of the second liquid at a specific ultraviolet light absorption peak (for example, the ultraviolet light absorption peak is 280nm), so as to determine the content of the purified protein according to the ultraviolet light absorption value.
  • the third detection component 163 when detecting the impurity content in the first liquid or the purified target content in the second liquid, the third detection component 163 may not be necessary, and other detection components may be used for detection.
  • the waste liquid temporary storage container 146 is provided with a specific content detection sensor, which can be used to detect the content of the purification target substance in the waste liquid temporary storage container 146 .
  • the collection container 145 is provided with a specific content detection sensor for detecting the content of the purified target substance in the collection container 145 .
  • the detection component may further include a fourth detection component 164 .
  • the fourth detection component 164 may refer to an instrument or device that detects whether the liquid level (ie, the liquid level) in the container reaches a set liquid level.
  • the fourth detection component 164 can be used to detect whether the liquid level in the temporary waste liquid storage container 146 and/or the collection container 145 reaches a set position.
  • the fourth detection component 164 may be disposed on the liquid filling head 143 of the collection component 14 .
  • the fourth detection component 164 may be disposed on the third plate body 1433 and configured such that when the liquid filling port 141 is located at the upper opening of the collection container 145 , the fourth detection component 164 is also located at the upper opening of the collection container 145 .
  • the liquid filling head 143 moves along the fourth track 144 , it can drive the fourth detection component 164 to move to the upper opening of the temporary waste liquid storage container 146 or the collection container 145 to detect the liquid level position thereof.
  • the fourth detection component 164 can be used to detect whether the liquid level in the collection container 145 reaches the set third liquid level threshold. In some cases, when the liquid in one of the collection containers 145 reaches the third liquid level threshold, the liquid filling port 141 may stop liquid filling to prevent the liquid in the collection container 145 from overflowing. In some embodiments, the fourth detection component 164 can be used to detect whether the liquid level in the temporary waste liquid storage container 146 reaches the fourth liquid level threshold. When the liquid in the waste liquid temporary storage container 146 reaches the fourth liquid level threshold, the liquid can be discharged to an external container with a larger volume through the discharge pipeline arranged at the bottom of the waste liquid to avoid overflow of the waste liquid.
  • the fourth detection component 164 may include one or more liquid level sensors as described in the previous embodiments. In some specific embodiments, the fourth detection component 164 may include an ultrasonic liquid level sensor.
  • the purification device 10 can include a fifth detection component 165, and the fifth detection component 165 can include a first photoelectric sensor 1651, and the first photoelectric sensor 1651 can be used to detect whether the first gravity column is installed in the support frame 1363 1313 or the second gravity column 1314.
  • the fifth detection component 165 includes two first photoelectric sensors 1651 and two spring pins 1652 .
  • the two first photoelectric sensors 1651 are symmetrically arranged under the slot 1366 of the holder.
  • Two installation holes 1653 are provided at the position corresponding to the first photoelectric sensor 1651 in the holder slot 1366 , and the central axes of the two installation holes 1653 are parallel to the height direction of the gravity column 1311 .
  • a spring 1654 is respectively disposed in the two installation holes 1653 .
  • one of the two spring pins 1652 may be called a first spring pin, and the other may be called a second spring pin.
  • One installation hole 1653 of the two installation holes 1653 may be called a first installation hole, and the other may be called a second installation hole.
  • One of the two springs 1654 may be called a first spring, and the other spring 1654 may be called a second spring.
  • the first spring pin passes through the first The spring and the upper end of the first spring abut against the first spring pin
  • the second spring pin passes through the second spring and the upper end of the second spring abuts against the second spring pin.
  • the spring pin 1652 can move along the central axis of the mounting hole 1653 through the spring 1654 .
  • the lower ends of the two guide strips 13632 correspond to the two mounting holes 1653 respectively, and a groove is provided on the lower end surface of one of the guide strips 13632.
  • one of the two guide bars 13632 may be called a first guide bar, and the other may be called a second guide bar.
  • the lower end of the first guide bar is provided with a groove.
  • the lower end of the guide bar 13632 corresponding to the installation hole 1653 may mean that the lower end of the guide bar 13632 is aligned with the installation hole 1653 so that the lower end of the guide bar 13632 can abut against the spring pin 1652 .
  • the lower end of the first guide bar when the first gravity column 1313 is matched with the support frame 1363 and put into the support frame slot 1366, the lower end of the first guide bar can be aligned with the first installation hole, and the lower end of the second guide bar can be Align with the second mounting hole.
  • the second gravity column 1314 when the second gravity column 1314 is mated with the support frame 1363 and put into the support frame slot 1366, the lower end of the first guide bar can be aligned with the second mounting hole, and the lower end of the second guide bar can be Align with the first mounting hole.
  • the upper ends of the spring pins 1652 protrude from the mounting holes 1653 .
  • the first guide bar can abut against the upper end of the first spring pin under the gravity of the support frame 1363 and the first gravity column 1313 and push the first The spring pin presses the first spring to make the first spring compress, and then makes the lower end of the first spring pin contact with the corresponding first photoelectric sensor 1651 .
  • the second guide bar can abut against the upper end of the second spring pin and push the second spring pin under the action of gravity of the support frame 1363 and the second gravity column 1314.
  • the two spring pins press the second spring to make the second spring compress, and then make the lower end of the second spring pin contact with the corresponding first photoelectric sensor 1651 . Therefore, when the first photoelectric sensor 1651 corresponding to the first spring pin generates a photoelectric sensing signal, it indicates that the support frame 1363 is placed in the support frame slot 1366 and the first gravity column 1313 is installed in the support frame 1363 .
  • the first photoelectric sensor 1651 corresponding to the second spring pin When the first photoelectric sensor 1651 corresponding to the second spring pin generates a photoelectric sensing signal, it indicates that the support frame 1363 is placed in the support frame slot 1366 and the second gravity column 1314 is installed in the support frame 1363 . When neither of the two first photoelectric sensors 1651 generates a photoelectric sensing signal, the support frame 1363 and the gravity column 1311 are not placed in the support frame slot 1366 .
  • the purification device 10 may further include a sixth detection component 166, and the sixth detection component 166 may be used to detect whether the first collection container support 1481 or the second collection container support 1481 is installed on the base 147. Two collection vessel holders 1482.
  • the sixth detection device 166 may include a second photoelectric sensor 1661 , and the second photoelectric sensor 1661 may be used to detect whether the first collection container support 1481 or the second collection container support 1482 is installed on the base 147 .
  • the sixth detection component 166 may include a first photoelectric sensor 1662, a second photoelectric sensor 1663 and two second photoelectric sensors 1661, at least one end of the length direction of the first collection container support 1481 is provided with a first photoelectric sensor.
  • the sensing sheet 1662 , the first photoelectric sensing sheet 1662 is disposed at a first height position in the height direction of the first collection container support 1481 .
  • At least one end of the second collection container support 1482 in the length direction is provided with a second photoelectric sensor 1663
  • the second photoelectric sensor 1663 is disposed at a second height position in the height direction of the second collection container support 1482 .
  • the first height position and the second height position are different.
  • At least one end of the base 147 in the length direction is provided with two second photoelectric sensors 1661 , and the two second photoelectric sensors 1661 are respectively located at the third height position and the fourth height position of the base 147 in the height direction.
  • the length direction of the base 147 can be indicated by the arrow X2 in FIG. 35 .
  • the height direction of the base 147 can be indicated by an arrow Y2 in FIG. 35 .
  • the length direction of the base 147 is parallel to the length direction of the first collection container support 1481 .
  • the 1661 detects that the second photoelectric sensor 1661 can generate a sensing signal.
  • the second photoelectric sensor 1663 at the second height position can be detected by the second photoelectric sensor 1661 at the fourth height position, and the second photoelectric sensor 1661 can generate sensing signal.
  • the second photoelectric sensor 1661 at the third height position generates a photoelectric sensing signal
  • the second photoelectric sensor 1661 at the fourth height position generates a photoelectric sensing signal
  • it indicates that the second collection container support 1482 is installed on the base 147 When neither of the two second photoelectric sensors 1661 generates a photoelectric sensing signal, it indicates that the collection container support 148 is not installed on the base 147 or the collection container support 148 is not properly installed.
  • both ends of the first collection container support 1481 in the length direction are provided with a first photoelectric sensor 1662
  • both ends of the second collection container support 1482 in the length direction are provided with a second photoelectric sensor 1663 .
  • the photoelectric sensors at both ends of the first collection container support 1481 and the second collection container support 1482 can be detected by the second photoelectric sensor 1661, and there is no need to install the first collection container support 1481 and the second collection container in a specific direction. Bracket 1482, reducing installation difficulty.
  • the sixth detection device 166 may include a gravity detection sensor disposed on the base 147 , and the gravity detection sensor may determine whether the first collection container support 1481 or the second collection container support 1482 is installed on the base 147 .
  • the self-weight of the first collection container support 1481 is the same as the self-weight of the second collection container support 1482
  • the weight of the second collection container 1452 is greater than that of the first collection container 1451 .
  • one or more components of the purification device 10 may be manually operated to perform corresponding functions.
  • the shaking platform 111 can be manually controlled to shake the mixing container 112 .
  • the magnetic member 121 can be manually controlled to extend into the mixing container 112 to absorb the magnetic beads 15 .
  • the liquid filling head 143 and the liquid filling port 141 can be manually controlled to slide along the fourth track 144 , so as to move to the upper opening of the collection container 145 or the temporary waste liquid storage container 146 .
  • one or more components of purification apparatus 10 may be controlled by other components to perform corresponding functions.
  • the purification device 10 may further include a control component 18, which may be used to control one or more components in the foregoing embodiments to perform corresponding functions.
  • the control component 18 can control the mixing component 11 to perform a mixing operation, so that the magnetic beads 15 in the mixing container 112 are fully combined with the purification target.
  • the control assembly 18 can control the magnetic member 121 in the transfer assembly 12 to move from the inside of the mixing container 112 to the upper opening of the separation container 131 .
  • control assembly 18 may include a control unit 181 and a drive unit 182 .
  • the control unit 181 can be used to receive work instructions, which can be input by an operator to instruct the control component 18 to drive one or more components to perform corresponding functions.
  • the control unit 181 can generate corresponding instructions according to the work instructions, and send the instructions to the driving unit 182 .
  • drive unit 182 may be in drive connection with one or more components of purification apparatus 10 .
  • the driving unit 182 may receive instructions from the control unit 181, and drive corresponding components to perform specific functions according to the instructions.
  • the control unit 181 and the driving unit 182 will be further described below in conjunction with one or more components of the purification device 10 .
  • the control unit 181 may generate a first instruction according to the work instruction and send it to the driving unit 182 .
  • the first instruction corresponds to the vibrating platform 111 of the mixing component 11 , and can instruct the vibrating platform 111 to rotate and vibrate the mixing container 112 at a set speed within a set working time.
  • the driving unit 182 may drive the oscillation platform 111 to work after receiving the first instruction.
  • the drive unit 182 can control the motor of the oscillating platform 111 (for example, the oscillating drive motor 113 in FIG. 13 ) to drive the crankshaft (for example, the crankshaft 114 in FIG. 13 ) to move according to the instruction of the first command, and then drive the oscillating platform 111 to rotate. .
  • the control unit 181 may generate a second instruction according to the work instruction and send it to the driving unit 182 .
  • the second instruction corresponds to the transfer assembly 12 and the magnetic bead separation assembly 133, and can instruct the magnetic member 121 (for example, the magnetic rod 1211 and the magnetic rod cover 1212) of the transfer assembly 12 to move to the upper opening of the separation container 131, and instruct the magnetic beads
  • the separation component 133 separates the magnetic beads 15 from the magnetic member 121 .
  • the driving unit 182 can control the magnetic member 121 and the magnetic bead detachment assembly 133 to work according to the instruction of the first instruction.
  • the magnetic member 121 may include a magnetic rod 1211 and a magnetic rod casing 1212 , and the magnetic rod 1211 and the magnetic rod casing 1212 are slidably connected to the first rail 123 and the second rail 124 through a first slider and a second slider, respectively.
  • the driving unit 182 can drive the first slider and the second slider to move, thereby driving the magnetic bar 1211 and the magnetic bar jacket 1212 to move along the corresponding track to the positions shown in Fig. 18 to Fig. 21, so that the magnetic bead 15 is Separate from the magnetic rod cover 1212.
  • the control unit 181 may generate a third instruction according to the work instruction and send it to the drive unit 182 .
  • the third command corresponds to the flushing assembly 1330, and may instruct the flushing nozzle 1331 to spray a specific liquid at a certain rate.
  • the drive unit 182 may control the flushing nozzle 1331 to work according to the instruction of the third instruction.
  • the driving unit 182 can drive the pump valve assembly communicated with the flushing nozzle 1331 to work, and the first liquid is sprayed out through the flushing nozzle 1331 to flush the magnetic beads 15 .
  • control unit 181 may generate a fourth instruction according to the work instruction and send it to the driving unit 182 .
  • the fourth command corresponds to the flushing assembly 1330, and may instruct the flushing nozzle 1331 to spray a specific liquid at a certain rate.
  • the driving unit 182 may control the flushing nozzle 1331 to work according to the instruction of the fourth instruction.
  • the driving unit 182 can drive the pump valve assembly communicated with the flushing nozzle 1331 to work, and the second liquid is sprayed out through the flushing nozzle 1331 to flush the magnetic beads 15 .
  • the content detection sensor can generate a detection signal according to the content of a specific substance in the liquid in the liquid discharge line communicating with the separation container.
  • the control assembly 18 can also be used to control the driving unit 182 to drive the collection assembly 14 to collect the liquid in the separation container 131 according to the detection signal.
  • the content detection sensor can generate a first content detection signal according to the impurity content in the first liquid in the drain line communicated with the separation container and/or generate a second content from the purification target content in the second liquid heartbeat.
  • the control unit 181 may generate a fifth instruction based on the second content detection signal and send it to the driving unit 182 .
  • the fifth instruction corresponds to the collection assembly 14 and may instruct the collection assembly 14 to collect the second liquid from the separation container 131 .
  • the drive unit 182 After the drive unit 182 receives the fifth instruction, it can To control the collecting component 14 to work according to the instructions of the fifth instruction. Exemplarily, as shown in FIG.
  • the content detection sensor detects that the content of the purification target substance in the second liquid in the discharge pipeline 17 communicated with the separation container 131 reaches the second content threshold. Therefore, the control unit 181 can generate a fifth instruction, and the fifth instruction can instruct the collection assembly 14 to collect the second liquid.
  • the driving unit 182 can drive the liquid filling head 143 of the collection assembly 14 to move along the fourth track 144 to the upper opening of the collection container 145 , and drive the liquid filling port 141 to discharge the second liquid to the collection container 145 .
  • the purification device 10 may also include a host computer, and the host computer may refer to a computer capable of issuing manipulation commands (for example, work instructions), such as a computer, a tablet, and the like.
  • the upper computer can connect/communicate with the control component 18, and instruct the drive unit 182 to drive the corresponding component to work by sending a work instruction to the control unit 181.
  • the upper computer can be manually controlled, for example, the operator can manually operate the upper computer software in the upper computer to issue work instructions.
  • the host computer can control the purification device 10 through a control program or software.
  • the host computer may include a display device, such as a display screen.
  • the display screen can be used to present the detection results of one or more detection components in a specific form (for example, numbers, images, etc.).
  • the purification device 10 may further include a switch 19 , and the switch 19 may be used to realize the connection/communication between the host computer and the control component 18 .
  • control component 18 may also receive a detection signal sent by the fourth detection component 164 that the liquid level of the liquid in the collection container 145 reaches the third liquid level threshold.
  • the control unit 181 may generate a sixth instruction based on the detection signal, and the instruction may instruct the liquid filling port 141 to move from above the current collection container 145 to above another collection container 145 .
  • the drive unit 182 can drive the liquid filling head 143 to move along the fourth track 144 , thereby driving the liquid filling port 141 to move to a corresponding position.
  • multiple purification devices 10 described in the foregoing embodiments may be connected in parallel to form a new first purification device group 100 .
  • the first purification equipment group 100 is composed of four purification equipment 10 connected in parallel.
  • the plurality of purification devices 10 in the first purification device group 100 are independent of each other and do not affect each other during the purification process.
  • one or more purification devices 10 of the first purification device group 100 may be controlled by the same host computer.
  • one or more purification devices 10 of the first purification device group 100 may be controlled by different host computers. It should be noted that the first purification equipment group 100 shown in FIG.
  • each purification device 10 of the first set of purification devices 100 may be identical.
  • the number of components of a single purification device 10 can also be increased.
  • the second purification equipment group 200 may include the same components as the purification equipment 10 in other embodiments of this specification.
  • the shaking platform 111 of the second purification equipment group 200, the mixing container 112, the separation assembly 13 (for example, the separation container 131), the collection assembly 14, the control assembly 18, the detection assembly (for example, the third detection assembly 163), the magnetic The bead detachment assembly 133 and the transfer assembly 12 (for example, the magnetic piece 121) can be connected with the shaking platform 111 of the purification device 10, the mixing container 112, the separation assembly 13 (for example, the separation container 131), the collection assembly 14, the control assembly 18, the detection Components (eg, third detection component 163 ), magnetic bead detachment component 133 , and transfer component 12 (eg, magnetic member 121 ) are the same or similar.
  • the shaking platform 111 of the second purification equipment group 200 can fix two mixing containers 112, and is provided with two separation components 13, two collection components 14, two transfer components 12 and two detection components.
  • the components of the second purification equipment group 200 can be independently controlled by the control component 18, and do not affect each other during work. In some cases, by increasing the number of components, the purification efficiency of the second purification equipment group 200 is improved, and the second purification equipment group 200 can also be made more compact in structure and smaller in size.
  • the expansion to 2 channels shown in FIG. 38 of this embodiment does not mean that the present invention is limited to the expansion to 2 channels, and the number of channels that can be expanded is not limited.
  • Figure 39 is an exemplary flow chart of a purification method according to some embodiments of the present specification. As shown in FIG. 39 , this specification also provides a method for purifying biomacromolecules in culture fluid based on the purification device 10 described in one or more of the foregoing embodiments.
  • the method 3900 may include the following steps:
  • Step 3910 control the mixing component 11 to perform a mixing operation.
  • controlling the mixing component 11 to perform the mixing operation includes: first putting a certain amount of culture solution and a certain amount of magnetic beads 15 into the mixing container 112, then fixing the mixing container 112 on the shaking platform 111 and The vibration platform 111 is controlled to vibrate the mixing container 112 to make the magnetic beads 15 combine with the purified target substance in the culture medium.
  • Step 3920 control the magnetic member 121 in the transfer assembly 12 to transfer the magnetic beads 15 to the upper opening of the separation container 131 .
  • controlling the magnetic piece 121 in the transfer assembly 12 to transfer the magnetic beads 15 to the upper opening of the separation container 131 includes: protruding a part of the magnetic piece 121 below the liquid surface of the mixing container 112, using the magnetic piece The adsorption force of 121 makes the magnetic beads 15 enriched on the surface of the magnetic element 121 . The magnetic piece 121 is then taken out from the mixing container 112 and moved to the separation container 131 opening above.
  • Step 3930 control the magnetic bead detachment component 133 to detach the magnetic beads 15 from the magnetic member 121 , so that the magnetic beads 15 enter the separation container 131 and accumulate on the filter member 132 .
  • Step 3940 control the flushing component 1330 to add the first liquid into the separation container 131 . Impurities not bound to the magnetic beads 15 are removed by washing the magnetic beads 15 with the first liquid.
  • Step 3950 control the flushing component 1330 to add the second liquid into the separation container 131 . Washing the magnetic beads 15 with the second liquid separates the purification target compound bound to the magnetic beads 15 from the magnetic beads 15 .
  • Step 3960 control the collecting component 14 to collect the first liquid and/or the second liquid from the separation container 131 .
  • the second liquid mixed with the purification target can be collected through the collection component 14 communicating with the lower opening of the separation container 131 .
  • the collection component 14 can be controlled to collect the first liquid from the separation container 131 .
  • the first liquid mixed with impurities may be collected through the collection component 14 communicating with the lower opening of the separation container 131 .
  • the magnetic member 121 may include a magnetic bar 1211 .
  • the magnetic bar 1211 can be controlled to slide along the first track 123 and the second track 124, so as to standardize the movement path of the magnetic bar 1211 and improve the stability during the movement sex.
  • the magnetic beads 15 can be washed by the washing assembly 1330 of the magnetic bead detachment assembly 133 arranged at the upper opening of the separation container 131 to remove the magnetic beads.
  • the beads 15 are separated from the magnetic member 121 .
  • the magnetic force of the magnetic member 121 can also be reduced to reduce the magnetic force of the magnetic member 121 on the magnetic bead 15 , so that the magnetic bead 15 is separated from the magnetic member 121 under the action of gravity.
  • the magnetic bar 1211 and the magnetic bar cover 1212 can be controlled to move to the positions shown in Fig. 18 to Fig.
  • the third detection component 163 can be used to detect the content of impurities in the first liquid in the drain line 17 communicating with the separation container 131 .
  • the impurity content in the first liquid reaches the first content threshold and the detection time threshold remains unchanged (that is, there is no change or almost no change), it indicates that the impurity washing is completed, and the addition of the first liquid can be stopped.
  • the third detection component 163 can be used to detect the content of the purification target substance in the second liquid in the drain line 17 communicated with the separation container 131 .
  • the second content threshold indicates that the content of the purification target substance in the second liquid meets the collection requirement, and the second liquid can be collected.
  • the purification target substance content reaches the third content threshold value, it shows that the purification target substance separated from the magnetic beads is less, and it can be considered that the purification target substance remaining on the magnetic beads 15 is less or completely non-residual, so the separation container 131 can be stopped. Add the second liquid and end the collection of the second liquid.
  • the third content threshold may exceed the second content threshold.
  • the third content threshold exceeds the second content threshold, if the content of the purification target reaches the third content threshold, and the content of the purification target in the second liquid is in a downward trend (for example, it can be based on the purification target corresponding to several consecutive moments content value (such as 10 numerical values), to determine the current trend of the purification target content), then it shows that the remaining purification target on the magnetic beads 15 is less or completely non-residual, so it is possible to stop adding the second purification target to the separation container 131. liquid and ends the collection of the second liquid. If the content of the purified target substance is not in a downward trend, the second liquid may continue to be collected.
  • the third content threshold may be less than the second content threshold.
  • the third content threshold is less than the second content threshold, if the content of the purification target reaches the third content threshold, it can be directly determined to stop adding the second liquid to the separation container 131 and end the collection of the second liquid.
  • the purification process can be considered as finished.
  • the rinsing component 1330 (for example, the rinsing nozzle 1331 ) can be controlled to add other liquids (including but not limited to PBS cleaning solution, pure water, sodium hydroxide solution) into the separation container 131 .
  • PBS cleaning solution pure water, sodium hydroxide solution
  • adding PBS detergent, pure water or sodium hydroxide solution, etc. to clean and disinfect the inside of the separation container 131 , the filter element 132 , the drain line 17 and the magnetic beads 15 .
  • parts such as the magnetic rod casing 1212 and the funnel 134 are soaked in sodium hydroxide solution for disinfection.
  • the purification method described in one or more embodiments above can be implemented through the control component 18 .
  • details about how the control component 18 controls one or more components can refer to the relevant embodiment in FIG. 37 , and details are not repeated here.
  • the possible beneficial effects of the culture device of the embodiment of this specification include but are not limited to: (1) by setting a filter in the separation container, the magnetic beads are separated from the liquid after washing the magnetic beads by the filter, so as to facilitate the mixing The liquid with purification target is collected, which effectively improves the purification efficiency; (2) by setting the first track (corresponding to the magnetic bar), the second track (corresponding to the magnetic bar coat), and the third track (corresponding to the magnetic bar coat) and magnetic rod), the fourth track (corresponding to the liquid head), standardize the operation of the corresponding parts (3) By setting a flushing nozzle above the separation container and using the liquid ejected from the flushing nozzle to flush the magnetic beads, the transfer efficiency of the magnetic beads can be accelerated, thereby improving the purification efficiency; (4) By setting a rotary table that rotates around the central axis of the separation container above the separation container, and setting a flushing nozzle on the rotary table, the rotary table is used to drive the flushing nozzle to flush the side walls of the magnetic parts in all directions, speeding
  • Various parameters in the purification process can be detected by setting detection components, for example, by detecting whether the liquid level in the separation container reaches the set position, and then the liquid The surface position is controlled in an appropriate range, which can effectively improve the purification efficiency; (6) By setting the separation container as openings at both ends, the magnetic beads can enter from one end opening, and impurities and purification targets are mixed during the purification process.
  • the liquid can be discharged from its other end opening, which improves the collection efficiency of the liquid in the separation container; (7) when the purification equipment provided by the invention is used for purification, it can save the cost of expensive consumables required for filtering and removing impurities from the crude sample, and Save the cost of consumables that need to be replaced multiple times in the conventional magnetic bead purification step; (8)
  • the crude sample volume that can be purified by the purification equipment provided by the present invention will not be restricted, and the sample volume above 1 liter can be purified; ( 9) When using the purification equipment provided by the present invention to purify the protein in the culture solution (i.e. the crude sample), the steps of centrifuging and filtering the culture solution can be omitted, which avoids the loss of the purified target substance in the culture solution during centrifugation and filtration , effectively improving the recovery rate of the purified target.
  • aspects of this specification may be illustrated and described in several patentable categories or situations, including any new and useful process, machine, product or combination of substances or their combination Any new and useful improvements.
  • various aspects of this specification may be entirely executed by hardware, may be entirely executed by software (including firmware, resident software, microcode, etc.), or may be executed by a combination of hardware and software.
  • the above hardware or software may be referred to as “block”, “module”, “engine”, “unit”, “component” or “system”.
  • aspects of this specification may be embodied as a computer product comprising computer readable program code on one or more computer readable media.
  • numbers describing the quantity of components and attributes are used. It should be understood that such numbers used in the description of the embodiments use modifiers such as "about”, “approximately” or “substantially” in some examples. to modify. Unless otherwise stated, “about”, “approximately” or “substantially” indicates that the stated figure allows for a variation of ⁇ 20%. Accordingly, in some embodiments, the numerical data used in the specification and claims are approximations that can vary depending upon the desired characteristics of individual embodiments. In some embodiments, numerical data should take into account the specified significant digits and adopt the general digit reservation method. Although the numerical ranges and data used in certain embodiments of this specification to confirm the breadth of the ranges are approximations, in specific embodiments, such numerical values are set as precisely as practicable.

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Abstract

一种分离组件、纯化设备及控制方法，其中纯化设备10包括混合组件11、转移组件12、分离组件13，混合组件包括震荡平台111；转移组件包括磁性件121；分离组件包括分离容器131和设置于分离容器中的过滤件132，过滤件开设有过滤孔，过滤孔的孔径小于磁珠的直径。还涉及一种控制方法，控制混合组件、转移组件、分离组件工作。

Description

分离组件、纯化设备及控制方法
优先权信息
本发明要求2022年01月17日提交的申请号为202210049101.2的中国专利申请的优先权，其全部内容通过引用并入本文。
技术领域
本说明书涉及生物大分子纯化技术领域，尤其涉及一种分离组件、纯化设备及控制方法。
背景技术
生物大分子(例如，抗体、蛋白、核酸等)的纯化是分子生物学、医学研究中的重要环节之一，纯化的效果直接影响研究和诊断的进程和结果。目前，抗体、蛋白等的纯化主要是通过层析柱纯化设备进行纯化，基于层析柱的纯化设备包括层析柱，层析柱可以用于将培养液(即粗样品)中的纯化目标物与培养液分离以及用于将纯化目标物与非纯化目标物(或称为杂质)分离，从而实现纯化目标物的提纯。一般层析柱的两端均设置有开口，层析柱的内部腔体中填充有填料，填料可以用于与纯化目标物混合或结合。利用该设备进行纯化之前，需要对粗样品进行离心和过滤去除细胞碎片等杂质，避免造成层析柱堵塞，操作比较麻烦，整体耗时较长，并且纯化体积较小，纯化效果较差。
针对上述问题，提供一种纯化设备，在提高纯化速度和纯化效果的同时可以实现大体积样本量的纯化。
发明内容
本说明书的之一在于提供一种纯化设备，所述纯化设备包括：混合组件，所述混合组件包括震荡平台；转移组件，所述转移组件包括磁性件；分离组件，所述分离组件包括分离容器和设置于所述分离容器中的过滤件，所述过滤件开设有过滤孔，所述过滤孔的孔径小于磁珠的直径。
在一些实施例中，所述磁性件包括磁棒，所述磁棒可沿所述震荡平台的高度方向运动。
在一些实施例中，所述转移组件还包括磁棒外套，所述磁棒外套套设在所述磁棒上，且所述磁棒外套能够相对所述磁棒沿套设方向运动。
在一些实施例中，所述转移组件还包括沿所述震荡平台的高度方向设置的第一轨道，所述磁棒可沿所述第一轨道滑动。
在一些实施例中，所述转移组件还包括沿所述震荡平台的高度方向设置的第一轨道和沿所述套设方向设置的第二轨道，所述第一轨道与所述磁棒外套相对固定，所述磁棒可沿所述第一轨道滑动，所述磁棒外套可沿所述第二轨道滑动。
在一些实施例中，所述纯化设备还包括防滴挡片，所述防滴挡片可伸缩地设置于所述第二轨道的底部。
在一些实施例中，所述转移组件还包括沿所述震荡平台的上方至所述分离容器的上方方向设置的第三轨道，所述磁棒可沿所述第三轨道滑动。
在一些实施例中，所述纯化设备还包括磁珠脱离组件，所述磁珠脱离组件用于将富集在所述磁性件上的所述磁珠从所述磁性件上脱离。
在一些实施例中，所述磁珠脱离组件包括冲洗组件；所述冲洗组件包括冲洗喷头，所述冲洗喷头设置在所述分离容器的上方。
在一些实施例中，所述冲洗组件还包括能够绕所述分离容器的中心轴线转动的旋转台，所述冲洗喷头设置于所述旋转台上。
在一些实施例中，所述分离容器的上方还设置有漏斗；所述漏斗的窄口端与所述分离容器的上方开口连通；所述旋转台能够绕所述漏斗的广口端转动。
在一些实施例中，所述分离容器包括重力柱，所述重力柱的上方开口与所述漏斗连通；所述分离组件还包括密封塞，所述重力柱的下方开口通过所述密封塞密封；与所述重力柱连通的排液管路的入口设置有穿刺针管，所述穿刺针管能够穿过所述密封塞。
在一些实施例中，所述分离组件还包括：支撑架，所述重力柱可以放置于所述支撑架内；适配结构，所述适配结构用于将不同的所述重力柱与所述支撑架进行配接。
在一些实施例中，所述适配结构包括：卡扣，设于所述支撑架的上方开口，用于限制所述重力柱晃动；压头，设于所述支撑架的下方开口，所述压头设有液体通道，所述压头的上方包括第一抵接面，所述液体通道的上方开口设于所述第一抵接面，所述液体通道的下方开口通过所述密封塞密封；所述重力柱的下方开口端包括与所述第一抵接面适配的第二抵接面，所述第一抵接面与所述第二抵接面抵接时，所述重力柱的下方开口伸入所述液体通道中。
在一些实施例中，所述漏斗的窄口端设置有压紧法兰，所述压紧法兰与所述卡扣配接时，将所述重力柱抵接于所述压头上。
在一些实施例中，所述重力柱包括不同规格的第一重力柱或第二重力柱，所述卡扣包括第一卡扣或第二卡扣，所述压头包括第一压头或第二压头；所述第一卡扣、所述第一压头与所述第一重力柱适配，所述第二卡扣、所述第二压头与所述第二重力柱适配。
在一些实施例中，所述旋转台可沿所述重力柱的高度方向运动。
在一些实施例中，所述分离组件还包括沿所述重力柱的高度方向设置的第一升降轨道，所述旋转台可沿所述第一升降轨道滑动。
在一些实施例中，所述支撑架可沿所述重力柱的高度方向移动。
在一些实施例中，所述分离组件还包括沿所述重力柱的高度方向设置的第二升降轨道，所述支撑架可沿所述第二升降轨道滑动。
在一些实施例中，所述支撑架可沿垂直于所述重力柱的高度方向移动。
在一些实施例中，所述分离组件还包括沿垂直于所述重力柱的高度方向设置的平移轨道，所述支撑架可沿所述平移轨道滑动。
在一些实施例中，所述纯化设备还包括第一检测组件；所述第一检测组件包括液位传感器，所述液位传感器用于检测所述分离容器中的液面位置是否达到设定的位置。
在一些实施例中，所述纯化设备还包括第一检测组件；所述第一检测组件包括液位传感器，所述液位传感器用于检测所述分离容器中的液面位置是否达到设定的位置。
在一些实施例中，所述第一检测组件还包括第二液位传感器，所述第二液位传感器设置在所述分离容器的第二位置，所述第一位置的高度高于所述第二位置的高度。
在一些实施例中，所述纯化设备还包括第二检测组件，所述第二检测组件用于检测加入到所述分离容器中的第一液体的体积。
在一些实施例中，所述纯化设备还包括第三检测组件，所述第三检测组件用于检测与所述分离容器连通的排液管路中第一液体中的杂质含量和/或第二液体中的纯化目标物含量。
在一些实施例中，所述第三检测组件包括与所述排液管路连通的含量检测传感器。
在一些实施例中，所述含量检测传感器包括紫外吸收检测器。
在一些实施例中，所述纯化设备还包括收集组件，所述收集组件用于收集来自所述分离容器的液体。
在一些实施例中，所述收集组件包括与所述分离容器的下方开口连通的加液口以及收集容器。
在一些实施例中，所述收集组件还包括废液暂存容器。
在一些实施例中，所述纯化设备还包括第四检测组件，用于检测所述废液暂存容器和/或收集容器中的液面位置是否达到设定的位置。
在一些实施例中，所述纯化设备包括第五检测组件，所述第五检测组件包括第一光电传感器，所述第一光电传感器用于检测所述支撑架内是否安装有所述第一重力柱或者所述第二重力柱。
在一些实施例中，所述收集组件还包括底座和收集容器支架，所述收集容器支架用于放置所述收集容器，所述收集容器支架安装于所述底座。
在一些实施例中，所述收集容器包括不同规格的第一收集容器或第二收集容器，所述收集容器支架包括第一收集容器支架或第二收集容器支架，所述第一收集容器支架用于放置所述第一收集容器，所述第二收集容器支架用于放置所述第二收集容器，所述第一收集容器支架的尺寸与所述第二收集容器支架的尺寸相同。
在一些实施例中，所述纯化设备还包括第六检测组件，所述第六检测组件包括第二光电传感器，所述第二光电传感器用于检测所述底座上是否安装有所述第一收集容器支架或者所述第二收集容器支架。
在一些实施例中，所述收集组件还包括沿所述废液暂存容器上方至所述收集容器上方方向设置的第四轨道，所述加液口可沿所述第四轨道滑动。
在一些实施例中，所述纯化设备还包括控制组件；所述控制组件用于控制所述混合组件进行混匀操作、控制所述转移组件中的所述磁性件运动至所述分离容器的上方开口。
在一些实施例中，所述混合组件还包括混匀容器，所述混匀容器设置于所述震荡平台上；所述控制组件包括驱动单元和控制单元，所述驱动单元与所述震荡平台驱动连接；所述控制单元用于生成第一指令，所述驱动单元用于根据所述第一指令驱动所述震荡平台震荡所述混匀容器。
在一些实施例中，所述纯化设备还包括磁珠脱离组件；所述驱动单元还与所述磁珠脱离组件以及所述磁性件驱动连接；所述控制单元还用于生成第二指令，所述驱动单元还用于根据所述第二指令驱动所述磁性件将所述磁珠转移至所述分离容器的上方开口以及驱动所述磁珠脱离组件将所述磁珠从所述磁性件上脱离。
在一些实施例中，所述磁珠脱离组件包括冲洗组件；所述驱动单元还与所述冲洗组件驱动连接；所述控制单元还用于生成第三指令，所述驱动单元还用于根据所述第三指令驱动所述冲洗组件向所述分离容器加入第一液体。
在一些实施例中，所述控制单元还用于生成第四指令，所述驱动单元还用于根据所述第四指令驱动所述冲洗组件向所述分离容器加入第二液体。
在一些实施例中，所述纯化设备还包括含量检测传感器和收集组件，所述驱动单元还与所述收集组件驱动连接；所述含量检测传感器用于根据与所述分离容器连通的排液管路中第一液体中的杂质含量生成第一含量检测信号和/或第二液体中的纯化目标物含量生成第二含量检测信号；所述控制单元还用于根据所述第二含量检测信号控制所述驱动单元驱动所述收集组件收集来自所述分离容器内的所述第二液体。
本说明书的之一在于提供一种分离组件，所述分离组件包括分离容器和设置于所述分离容器中的过滤件，所述过滤件开设有过滤孔，所述过滤孔的孔径小于磁珠的直径。
在一些实施例中，所述分离容器的两端均设置有开口。
在一些实施例中，所述过滤件包括过滤板，所述过滤板的厚度方向与所述分离容器的高度方向平行，所述过滤板沿其厚度方向的横截面形状轮廓与所述分离容器的内部腔体沿其高度方向的横截面轮廓匹配。
本说明书的之一在于提供一种基于纯化设备的控制方法，所述纯化设备包括：混合组件，所述混合组件包括震荡平台；转移组件，所述转移组件包括磁性件；分离组件，所述分离组件包括分离容器和设置于所述分离容器中的过滤件，所述过滤件开设有过滤孔，所述过滤孔的孔径小于磁珠的直径；磁珠脱离组件，所述磁珠脱离组件包括冲洗组件；收集组件，用于收集来自所述分离容器的液体；所述控制方法包括：控制所述混合组件进行混匀操作；控制所述转移组件中的所述磁性件将所述磁珠转移至所述分离容器的上方开口；控制所述磁珠脱离组件将所述磁珠从所述磁性件上脱离，使所述磁珠进入所述分离容器并堆积于所述过滤件上；控制所述冲洗组件向所述分离容器内加入第一液体；控制所述冲洗组件向所述分离容器内加入第二液体；控制所述收集组件收集来自所述分离容器的所述第一液体和/或所述第二液体。
在一些实施例中，所述磁性件包括磁棒，所述转移组件包括沿所述震荡平台的高度方向设置的第一轨道以及沿所述震荡平台的上方至所述分离容器的上方方向设置的第三轨道，所述磁棒可沿所述第一轨道和所述第三轨道滑动；所述控制所述转移组件中的所述磁性件将所述磁珠转移至所述分离容器的上方开口包括：控制所述磁棒沿所述第一轨道和所述第三轨道滑动，将所述磁珠转移至所述分离容器的上方开口。
在一些实施例中，所述控制所述磁珠脱离组件将所述磁珠从所述磁性件上脱离包括：控制所述冲洗组件冲洗所述磁珠。
在一些实施例中，所述纯化设备还包括第三检测组件，所述第三检测组件用于检测与所述分离容器连通的排液管路中所述第一液体中的杂质含量；在控制所述冲洗组件向所述分离容器内加入第二液体之前，还包括：控制所述第三检测组件检测与所述分离容器连通的所述排液管路中所述第一液体中的杂质含量；当所述第一液体中的杂质含量达到第一含量阈值且维持检测时间阈值不变时，控制所述冲洗组件停止加入所述第一液体。
在一些实施例中，所述第三检测组件还用于检测与所述分离容器连通的所述排液管路中所述第二液体中的纯化目标物含量；所述控制所述收集组件收集来自所述分离容器的所述第一液体和/或第二液体包括：控制所述第三检测组件检测与所述分离容器连通的所述排液管路中所述第二液体中的所述纯化目标物含量。
附图说明
本说明书将以示例性实施例的方式进一步说明，这些示例性实施例将通过附图进行详细描述。这些实施例并非限制性的，在这些实施例中，相同的编号表示类似的结构，其中：
本说明书将以示例性实施例的方式进一步说明，这些示例性实施例将通过附图进行详细描述。这些实施例并非限制性的，在这些实施例中，相同的编号表示相同的结构，其中：
图1是根据本说明书一些实施例所示的纯化设备的简易结构示意图；
图2是根据本说明书一些实施例所示的纯化设备的结构示意图；
图3是根据本说明书另一些实施例所示的纯化设备的结构示意图；
图4是图2所示的纯化设备在另一角度下的示意图；
图5是图3所示的纯化设备在另一角度下的示意图；
图6是图2所示的纯化设备在又一角度下的示意图；
图7是根据本说明书一些实施例所示的转移组件的结构示意图；
图8是根据本说明书另一些实施例所示的转移组件的结构示意图；
图9是图8所示的转移组件在另一角度下的示意图；
图10是根据本说明书一些实施例所示的磁性件位于混匀容器内的结构示意图；
图11是根据本说明书另一些实施例所示的磁性件位于混匀容器内的结构示意图；
图12是根据本说明书一些实施例所示的纯化设备的结构剖视图；
图13是根据本说明书另一些实施例所示的纯化设备的结构剖视图；
图14是根据本说明书一些实施例所示的磁性件将磁珠转移至混匀容器外部的结构示意图；
图15是根据本说明书另一些实施例所示的磁性件将磁珠转移至混匀容器外部的结构示意图；
图16是根据本说明书一些实施例所示的纯化设备在另一角度下的结构剖视图；
图17是根据本说明书另一些实施例所示的纯化设备在另一角度下的结构剖视图；
图18是根据本说明书一些实施例所示的磁性件将磁珠转移至分离容器的上方开口的结构示意图；
图19是根据本说明书另一些实施例所示的磁性件将磁珠转移至分离容器的上方开口的结构示意图；
图20是根据本说明书一些实施例所示的纯化设备在又一角度下的结构剖视图；
图21是根据本说明书另一些实施例所示的纯化设备在又一角度下的结构剖视图；
图22是根据本说明书一些实施例所示的分离组件与磁珠脱离组件连接的结构示意图；
图23是根据本说明书一些实施例所示的分离组件与磁珠脱离组件连接的剖视图；
图24是根据本说明书另一些实施例所示的分离组件与磁珠脱离组件连接的剖视图；
图25是根据本说明书又一些实施例所示的分离组件与磁珠脱离组件连接的结构示意图；
图26是图25所示的分离组件与磁珠脱离组件连接的结构的剖面示意图；
图27是根据本说明书一些实施例所示的穿刺针管从密封塞拔出的示意图；
图28是根据本说明书一些实施例所示的重力柱从支撑架中取出的示意图；
图29是根据本说明书一些实施例所示的重力柱与支撑架连接的结构示意图；
图30是根据本说明书一些实施例所示的第一重力柱和第二重力柱分别与支撑架连接的结构示意图；
图31是根据本说明书一些实施例所示的支撑架与支撑架卡槽连接的剖面示意图；
图32是图31中A处的局部放大示意图；
图33是根据本说明书一些实施例所示的收集组件的结构示意图；
图34是根据本说明书一些实施例所示的不同收集容器与收集容器支架连接的结构示意图；
图35是根据本说明书一些实施例所示的收集容器支架安装于底座的结构示意图；
图36是根据本说明书一些实施例所示的收集组件的结构示意图；
图37是根据本说明书一些实施例所示的第一纯化设备组的结构示意图；
图38是根据本说明书又一些实施例所示的第二纯化设备组的结构示意图；
图39是根据本说明书一些实施例所示的纯化方法的示例性流程图。
附图标记：10-纯化设备，11-混合组件，111-震荡平台，112-混匀容器，113-震荡驱动电机；114-曲轴；115-粘板；12-转移组件，121-磁性件，1211-磁棒，1212-磁棒外套，123-第一轨道，124- 第二轨道，125-第三轨道，126-防滴挡片，13-分离组件，131-分离容器，1311-重力柱，1312-第二抵接面，1313-第一重力柱，1314-第二重力柱，132-过滤件，133-磁珠脱离组件，1330-冲洗组件，1331-冲洗喷头，1332-旋转台，134-漏斗，1341-第一漏斗，1342-第二漏斗，1351-旋转电机，1352-带轮，1353-皮带，1361-密封塞，1362-穿刺针管，1363-支撑架，13631-观察窗，13632-导向条，1364-卡扣，13641-第一卡扣，13642-第二卡扣，1365-压头，13651-液体通道，13652-第一抵接面，13653-第一压头，13654-第二压头，1366-支撑架卡槽，13661-导向轴，1367-O型圈，13671-第一O型圈，13672-第二O型圈，1368-压紧法兰，1371-第一升降轨道，1372-第一升降电机，1373-第一升降丝杠，1381-第二升降电机，1391-平移轨道，1392-平移电机，1393-平移丝杠，14-收集组件，141-加液口，142-额外加液口，143-加液头，1431-第一板体，1432-第二板体，1433-第三板体，144-第四轨道，145-收集容器，1451-第一收集容器，1452-第二收集容器，146-废液暂存容器，147-底座，148-收集容器支架，1481-第一收集容器支架，1482-第二收集容器支架，15-磁珠，161-第一检测组件，1611-第一液位传感器，1612-第二液位传感器，162-第二检测组件，163-第三检测组件，164-第四检测组件，165-第五检测组件，1651-第一光电传感器，1652-弹簧销，1653-安装孔，1654-弹簧，166-第六检测组件，1661-第二光电传感器，1662-第一光电感应片，1663-第二光电感应片，17-排液管路，18-控制组件，181-控制单元，182-驱动单元，100-第一纯化设备组，200-第二纯化设备组。
具体实施方式
为了更清楚地说明本说明书的实施例的技术方案，下面将对实施例描述中所需要使用的附图作简单的介绍。显而易见地，下面描述中的附图仅仅是本说明书的一些示例或实施例，对于本领域的普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图将本说明书应用于其他类似情景。应当理解，给出这些示例性实施例仅仅是为了使相关领域的技术人员能够更好地理解进而实现本发明，而并非以任何方式限制本发明的范围。除非从语言环境中显而易见或另做说明，图中相同标号代表相同结构或操作。
如本说明书和权利要求书中所示，除非上下文明确提示例外情形，“一”、“一个”、“一种”和/或“该”等词并非特指单数，也可包括复数。一般说来，术语“包括”与“包含”仅提示包括已明确标识的步骤和元素，而这些步骤和元素不构成一个排它性的罗列，方法或者设备也可能包含其他的步骤或元素。术语“基于”是“至少部分地基于”。术语“一个实施例”表示“至少一个实施例”；术语“另一实施例”表示“至少一个另外的实施例”。其他术语的相关定义将在下文描述中给出。
图1是根据本说明书一些实施例所示的纯化设备的简易结构示意图。如图1所示，该纯化设备10可以包括混合组件11、转移组件12以及分离组件13。混合组件11可以用于将磁珠与纯化目标物混合或结合。转移组件12可以用于将磁珠转移至特定容器中，以便于对磁珠上的纯化目标物进行分离。分离组件13可以用于收容磁珠。其中，混合组件11可以包括震荡平台111。震荡平台111上可以用于放置混匀容器112，混匀容器112用于收容磁珠和培养液。震荡平台111可以以包括往复摆动、旋转等在内的形式进行运动，并带动放置在震荡平台111上的混匀容器112进行运动。磁珠和培养液中的纯化目标物可以在震荡平台111的运动下进行混合或结合。在一些实施例中，震荡平台111可以包括震荡驱动组件，用于驱动震荡平台111进行运动。仅作为示例，如图13所示，震荡驱动组件可以包括震荡驱动电机113、曲轴114和粘板115。粘板115可以用于放置混匀容器112，防止混匀容器112晃动。震荡驱动电机113与曲轴114传动连接，曲轴114与粘板115传动连接，震荡驱动电机113驱动曲轴114运动时，可以带动粘板115运动，从而带动混匀容器112进行运动。
磁珠(或称为超顺磁性纳米微球)可以是指能够与生物大分子(例如，核酸、蛋白、抗体等)相结合的具有磁响应性的纳米粒子。在一些实施例中，磁珠可以由硅基、PS基(即聚苯乙烯高分子材料)或琼脂糖等包裹。示例性的，磁珠的磁核材料可以是Fe3O4。在一些实施例中，磁珠的直径的取值范围可以包括1nm～500um。在一些实施例中，磁珠的直径的取值范围可以包括3nm～400um。在一些实施例中，磁珠的直径的取值范围可以包括5nm～300um。在一些实施例中，磁珠的直径的取值范围可以包括50nm～200um。在一些实施例中，可以根据结合不同功能基团来进行各种应用。功能基团包括但不限于Protein A、Protein G、Ni、GSH、oligo探针、链霉亲和素、抗体等单种或多种或夹心结构。在一些实施例中，磁珠可以应用于对生物大分子的纯化。纯化是指将粗样品(例如，培养液)中的纯化目标物与其他杂质分离，实现提纯。在一些实施例中，纯化目标物可以包括生物大分子(例如蛋白、抗体以及核酸等)。例如，当纯化目标物为抗体时，可以称为目标抗体。又例 如，当纯化目标物为细胞时，可以称为目标细胞。在一些实施例中，在纯化之前，需要将磁珠与纯化目标物混合。混合可以是指使纯化目标物与培养液分离，并与磁珠进行混杂。在一些实施例中，混合的方式包括但不限于物理方式混合(例如，让纯化目标物进入磁珠的三维网状孔径里面滞留)、化学键相结合或者通过离子交换、静电吸附等方式相结合等。将纯化目标物与磁珠混合的目的在于当纯化目标物与磁珠相结合之后，仅需要将磁珠从培养液中取出就相当于将纯化目标物从培养液中分离，实现初步提纯。
在一些实施例中，转移组件12可以包括磁性件121。磁性件121具有磁性，可以用于吸附混合或结合在培养液中的磁珠，以将磁珠与培养液分离。此外，磁性件121还可以用于将磁珠转移至特定容器(例如，分离容器131)的上方开口。分离组件13可以包括分离容器131，分离容器131中设置有过滤件132，过滤件132的过滤孔的孔径小于磁珠的直径。分离容器131的上方开口可以供由转移组件12转移的磁珠进入分离容器131的内部腔体，通过设置在内部腔体中的过滤件132对磁珠进行截留，从而达到收容磁珠的目的。
在一些实施例中，纯化设备10还可以包括控制组件18，控制组件18可以用于控制纯化设备10的一个或多个组件执行相应的功能。在一些实施例中，控制组件18可以包括控制单元181和驱动单元182。控制单元181可以生成指令并将命令发送给驱动单元182。驱动单元182可以根据指令驱动对应的组件执行相应的功能。关于控制组件18的更多描述可参见本说明书的其他部分。
在一些实施例中，在基于本说明书提供的纯化设备进行纯化的过程中，当磁珠被转移至分离容器131中后，可以依次向分离容器131中加入第一液体、第二液体等液体。第一液体可以用于对磁珠进行冲洗，以将未与磁珠混合或结合的杂质移除，实现杂质分离。因此利用第一液体冲洗磁珠的步骤也可称为洗杂处理。在一些实施例中，第一液体可以包括洗杂液，其包括Na2HPO4、KH2PO4、NaCl和KCl等。洗杂液可以根据纯化目标物的种类不同而不同，例如，当纯化目标物为核酸时，洗杂液可以包括三(羟甲基)氨基甲烷、甘露醇等。第二液体可以用于对磁珠进行冲洗，以将与磁珠结合的纯化目标物从磁珠上脱离。使其混合在第二液体中从分离容器131排出，便于收集提纯后的纯化目标物。因此利用第二液体冲洗磁珠的步骤可称为洗脱处理。在一些实施例中，第二液体可以包括洗脱液。洗脱液可以包括甘氨酸、柠檬酸盐或者醋酸钠等。洗脱液可以根据纯化目标物的种类不同而不同，例如，当纯化目标物为核酸时，洗脱液可以包括纯水或Tris-HCl。在一些情况下，本说明书提供的纯化设备10可以利用其设置在分离容器131中的过滤件132将磁珠与混合有杂质或者混合有纯化目标物的液体进行分离，使得对纯化目标物的收集更加方便。
在一些情况下，本说明书提供的纯化设备10不需要使用特制的磁珠过滤柱，也不需要在分离容器131中预装第二磁珠，而是直接将混合或结合有纯化目标物的磁珠转移至设置有过滤件132的分离容器131中进行纯化。同时无需利用滤膜对粗样品进行过滤，一定程度上减小了耗材成本。在另一些情况下，由于过滤件132的过滤孔的孔径小于磁珠的直径，因此可以将磁珠截留在分离容器131中，不需要使用第二磁珠的磁性来固定磁珠，使纯化操作更加简便，提高了纯化效率。同时该过滤件132还可以避免磁珠混合在第二液体中经由过滤孔132流出，有效提高了纯化效果。此外，由于本说明书提供的纯化设备10不需要利用滤膜对粗样品进行离心和过滤处理，因此可以避免离心和过滤对纯化目标物造成的损失，提高纯化目标物的回收率。
在一些实施例中，混匀容器112可以是任意具有容纳空间的容器。在一些具体实施例中，混匀容器112可以为适宜进行震荡旋转的容器。示例性的，如图2至图6所示，混匀容器112可以为摇瓶。在一些实施例中，为了防止震荡过程中对混匀容器112造成破坏，在混匀容器112和震荡平台111之间可以设置弹性件。示例性的弹性件可以包括弹簧、弹片以及硅胶垫等。在一些实施例中，混匀容器112的容积可以根据所要容纳的培养液的体积而定。示例性的，混匀容器112的容积的取值范围可以包括300ml～300L。
图10至图21分别示出了利用磁性件121将磁珠15从混匀容器112内部转移至分离容器131的上方开口的过程。该过程包括：将磁性件121伸入混匀容器112内部，并使一部分磁性件121没入液面以下(如图10和图13所示的位置)，对磁珠15进行吸附。控制磁性件121移动至混匀容器112外部(如图8和图9所示的位置)。控制磁性件121移动至分离容器131的上方开口(如图14和图17所示的位置)。本说明书不限制磁性件121的具体结构、尺寸，只要能够实现上述描述的功能即可。例如，在纯化过程中，需要将磁性件121伸入到不同结构(例如，混匀容器112、分离组件13的漏斗134等)中，因此需要控制磁性件121的外径。在一些实施例中，磁性件121的外径可以小于分离容器131的上方开口的口径。在一些实施例中，磁性件121的外径可以小于混匀容器112的上方开口的口径。在一些实施例中，磁性件121的外径可以小于漏斗134的广口端的口径。 在一些实施例中，磁性件121可以包括磁棒1211。如前述实施例描述，磁棒1211可以沿震荡平台111的高度方向运动。震荡平台111的高度方向也可以是指震荡平台111的厚度方向。在一些实施例中，当混匀容器112固定于震荡平台111上时，震荡平台111的高度方向可以与混匀容器112的开口至底部的方向平行或近似平行。为了方便描述，在本说明书中，震荡平台111的高度方向可以通过图4、图12和图16中的箭头Y来表示。
结合图7至图11所示，在一些实施例中，转移组件12还可以包括沿震荡平台111的高度方向设置的第一轨道123。磁棒1211可以沿第一轨道123滑动。示例性的，如图7所示，磁棒1211的顶部固定设置有与第一轨道123适配的第一滑动件。通过第一滑动件与第一轨道123配接使得磁棒1211能够沿第一轨道123滑动。在一些情况下，通过设置第一轨道123，可以规范磁棒1211的运动路径，提高磁棒1211在运动过程中的稳定性。
在一些实施例中，转移组件12还可以包括磁棒外套1212。磁棒外套1212可以套设在磁棒1211上，且磁棒外套1212能够相对磁棒1211沿套设方向运动。其中，磁棒外套1212可以是指套设在磁棒1211外的壳体结构。示例性的，磁棒外套1212的一端具有开口，另一端封闭。磁棒1211可以从磁棒外套1212的开口端嵌入磁棒外套1212内，并沿套设方向移动。套设方向可以是磁棒1211的长度方向。在一些实施例中，套设方向可以与震荡平台111的高度方向相同。在本实施例中，当磁棒1211与磁棒外套1212配接(即磁棒外套1212套设在磁棒1211外)并伸入混匀容器112的液面以下时，磁棒1211提供的吸附力会使得磁珠15富集在磁棒外套1212的表面。在一些情况下，套设在磁棒1211外的磁棒外套1212可以对磁棒1211进行保护，避免磁棒1211直接与培养液接触对磁棒1211造成腐蚀。在另一些情况下，由于磁棒外套1212能够相对磁棒1211沿套设方向运动，因此可以将磁棒外套1212与磁棒1211分离，便于清洗或更换。还有一些情况下，可以利用磁棒外套1212能够相对磁棒1211移动的特点，实现磁珠15与磁棒外套1212的分离。关于此，在本说明书其他实施例中有更详细的描述，此处不再赘述。
如图7所示，在一些实施例中，转移组件12沿震荡平台111的高度方向设置的第一轨道123和沿套设方向设置的第二轨道124。第一轨道123与磁棒外套1212相对固定。磁棒1211可沿第一轨道123滑动，磁棒外套1212可沿第二轨道124滑动。在一些情况下，通过设置第一轨道123和第二轨道124，可以同时规范磁棒1211和磁棒外套1212的移动路径，提高磁棒1211和磁棒外套1212在运动过程中的稳定性。
如图7所示，在一些实施例中，磁棒外套1212的开口端固定设置有与第二轨道124适配的第二滑动件，通过第二滑动件能够带动磁棒外套1212沿第二轨道124滑动。同时，第二滑动件背离第二轨道124的一面还设置有第一轨道123，使得第一轨道123与磁棒外套1212相对固定。如图7所示，在一些实施例中，磁棒外套1212的开口端固定设置有与第二轨道124适配的第二滑动件，通过第二滑动件能够带动磁棒外套1212沿第二轨道124滑动。同时，第二滑动件背离第二轨道124的一面还设置有第一轨道123，使得第一轨道123与磁棒外套1212相对固定。在一些实施例中，第二轨道124可以独立于第一轨道123设置。如图8和图9所示，第一轨道124和第二轨道123可以并列设置。
如图10、图14和图18所示，在一些实施例中，转移组件12还可以包括沿震荡平台111的上方至分离容器131的上方方向设置的第三轨道125。磁性件121(例如，磁棒1211)可以沿第三轨道125滑动。从震荡平台111的上方至分离容器131的上方方向可以是指，从固定于震荡平台111上的混匀容器112的上方开口至分离容器131的上方开口的方向。为了方便描述，该方向可以通过图4和图6中的箭头X表示。其中，震荡平台111的上方可以是指震荡平台111的正上方或斜上方，例如，图14至图17中磁棒1211和磁棒外套1212所处的位置。分离容器131的上方可以是指分离容器131的正上方或斜上方，例如，图10和图11中磁棒1211和磁棒外套1212所处的位置。在一些情况下，通过设置第三轨道125，可以规范磁棒1211和磁棒外套1212从震荡平台111上方移动至分离容器131上方过程中的运动路径，提高在运动过程中的稳定性。
需要说明的是，前述实施例对于磁棒1211沿着第一轨道123滑动、磁棒外套1212沿着第二轨道124滑动以及磁棒1211和磁棒外套1212沿着第三轨道125滑动的描述仅出于说明目的，并不旨在限制磁棒1211、磁棒外套1212的运动方式。在一些实施例中，磁棒1211、磁棒外套1212可以通过手动控制(即操作人员用手拿着)进行移动。在另一些实施例中，磁棒1211、磁棒外套1212可以通过驱动装置，例如，通过机械手控制磁棒1211、磁棒外套1212进行移动。
结合图14至图17所示，在一些实施例中，纯化设备10的转移组件12还可以包括防滴挡片126。防滴挡片126可伸缩地设置于第二轨道125的底部。在一些实施例中，防滴挡片126可以 被配置为当磁性件121位于防滴挡片126所在平面以上时时处于伸出状态，当磁性件121位于防滴挡片126所在平面以下时处于收缩状态。例如，当磁棒1211和磁棒外套1212分别沿各自的轨道移动到图14和图16所示的位置时，防滴挡片126可以伸出并位于磁棒外套1212的正下方。在磁性件121从混匀容器112外部移动至分离容器131的上方开口的过程中，防滴挡片126可以位于磁性件121的下方，用于接住磁珠15上所含的残余液体，防止残余液体滴落到纯化设备10的其他部件上造成污染。其中，磁性件121的下方可以是指磁性件121的正上方或斜下方。
结合图18至图24所示，在一些实施例中，纯化设备10还可以包括磁珠脱离组件133。磁珠脱离组件133可以用于将富集在磁性件121表面的磁珠15与磁性件121分离，使磁珠15进入分离容器131中，以便对磁珠15进行后续处理。在一些实施例中，磁珠脱离组件133可以包括冲洗组件1330。冲洗组件1330可以用于对吸附有磁珠15的磁性件121(例如，套设有磁棒1211的磁棒外套1212)进行冲洗，将磁珠15与磁性件121分离。在一些实施例中，冲洗组件1330可以包括冲洗喷头1331。冲洗喷头1331可以设置在分离容器131的上方。
在一些实施例中，冲洗喷头1331可以与外部的泵阀组件连通。泵阀组件可以用于使冲洗喷头1331喷出特定的液体(例如，第一液体、第二液体等)。在一些实施例中，冲洗喷头1331可以设置在分离容器131的上方任意位置，只要冲洗喷头1331朝向分离容器131的上方开口设置，以使喷出的液体能够流入到分离容器131内即可。例如，在图18至图24所示的实施例中，冲洗喷头1331可以设置在分离容器131的上端，其喷口倾斜向下朝向分离容器131的中心轴线。在一些应用场景中，冲洗喷头1331喷出特定的液体可以对磁棒外套1212上的磁珠15进行冲洗。使得磁珠15在液体的冲击下从磁棒外套1212上脱离进入到分离容器131内。在另一些应用场景中，冲洗喷头1331喷出的液体会流入到分离容器131中，对堆积在过滤件132上的磁珠15进行洗杂或洗脱。
在一些实施例中，冲洗组件1330可以包括多个冲洗喷头1331，多个冲洗喷头1331可以间隔环绕设置在分离容器131的上方，提高冲洗效率。
在一些实施例中，冲洗组件1330除了可以用于喷出第一液体和第二液体对磁珠15进行洗杂和洗脱之外，还可以用于对系统管路(例如，排液管路17)或磁珠15进行清洗。示例性的，冲洗喷头1331可以喷出PBS缓冲液、纯水或者氢氧化钠溶液到分离容器131中，对磁珠15以及组件内部(例如，分离容器131内壁、排液管路17等)进行清洗。其中，氢氧化钠溶液一方面可以去除管路中的内毒素，避免下一次纯化实验引入过多内毒素，影响下游实验结果。另一方面可以对磁珠15进行清洗再生，磁珠15清洗后可以用于下一次纯化实验，避免磁珠15浪费，同时防止交叉污染。需要说明的是，冲洗组件1330可以不是必须的。特定液体(例如，第一液体、第二液体或者其他类型的液体)的添加可以通过加液管路实现。例如，分离组件13的上方开口设置有加液管路，加液管路可以与泵阀组件连通，根据需要向分离容器131中加入特定的液体。在一些实施例中，加液管路也可以与冲洗组件1330相结合，两者可以分别喷出不同类型的液体。例如，冲洗组件1330喷出第一液体，加液管路用于加入第二液体。
在一些实施例中，冲洗组件1330还可以包括能够绕分离容器131的中心轴线转动的旋转台1332。冲洗喷头1331可以设置于旋转台1332上。在一些实施例中，旋转台1332可以为圆环状构件，圆环状构件可以套设在分离容器131的上方开口部分的外侧壁，并且能够相对分离容器131的外侧壁转动。
在一些应用场景中，由于磁棒外套1212的侧壁上均富集有磁珠15，若仅通过一个冲洗喷头1331进行冲洗，则磁棒外套1212的部分区域的磁珠15可能无法被冲洗，残留在磁棒外套1212上。若增加冲洗喷头1331的数量，冲洗喷头1331的数量过多可能会导致冲洗喷头1331喷出的液体压力不足，使得冲洗效果较弱。在一些情况下，通过在分离容器131上方设置旋转台1332，使旋转台1332带动冲洗喷头1331绕分离容器131的中心轴线转动，可以增大冲洗喷头1331的冲洗面积，提高磁珠15脱离的效率，进而提高纯化效率。需要说明的是，旋转台1332可以不是必须的，可以通过其他方式实现冲洗喷头1331相对磁性件121的转动。示例性的，磁性件121可以被配置为能够沿其中心轴线进行转动。通过磁性件121自身的转动，同样可以提高冲洗喷头1311的冲洗面积，加快磁珠15脱离。
在一些实施例中，冲洗组件1330还可以包括驱动旋转台1332绕分离容器131的中心轴线转动的旋转驱动组件。仅作为示例，结合图25和图26所示，旋转驱动组件包括旋转电机1351、带轮1352和皮带1353，带轮1352和旋转台1332通过皮带1353实现传动连接，旋转电机1351与带轮1352传动连接。当旋转电机1351驱动带轮1352旋转时，带轮1352能够通过皮带1353带动旋转台1332旋转。在另一些实施例中，旋转驱动组件还可以包括齿轮组件、螺杆螺母组件等。
在一些实施例中，除了利用冲洗组件1330对磁珠15进行冲洗使磁珠15从磁性件121(例如，磁棒外套1212)上脱离外，还可以利用磁性件121本身的特性来实现。例如，可以通过减小磁性件121对于磁珠15的吸附力(即磁力作用)，使得磁珠15在重力作用下与磁性件121脱离。
在一些实施例中，可以分别控制磁棒1211和磁棒外套1212沿对应的轨道滑动到图18至图21所示的位置。其中，磁棒外套1212沿第二轨道124滑动至该位置时，磁棒外套1212的底部位于分离组件13的漏斗134内。而磁棒1211则沿第一轨道123滑动至最上方，此时磁棒1211与磁棒外套1212的底部之间的距离最远。在本实施例中，由于磁棒1211与磁棒外套1212的底部距离较远，使得磁棒1211对于富集在磁棒外套1212的底部(即伸入到分离组件13的漏斗134中的部分)的磁珠15的吸附力几乎可以忽略。因此磁珠15可以在重力作用下从磁棒外套1212上脱离。在一些替代性实施例中，磁棒1211可以包括电磁棒。电磁棒通电时具有磁性；断电时，电磁棒磁性消失。在本实施例中，可以通过控制电磁棒的通/断电来控制电磁棒的磁性，进而控制磁性件121对磁珠15的吸附力。示例性的，当需要将磁珠15与磁性件121脱离时，可以停止对电磁棒通电。电磁棒断电后失去磁性，使得磁棒1211与磁珠15之间的作用力消失，磁珠15可以在重力作用下与磁棒外套1212分离。
在一些实施例中，可以将前述实施例中的冲洗组件1330与减小磁性件121对于磁珠15的吸附力相关的实施例相结合，加快磁珠15与磁性件121分离，提高纯化效率。示例性的，磁性件121可以包括磁棒1211和磁棒外套1212，磁棒外套1212和磁棒1211可以分别沿对应的轨道滑动至图18至图21所示的位置；在分离容器131的上方设置有冲洗喷头1331。在一些情况下，通过磁棒外套1212和磁棒1211的相对移动，可以降低磁性件121对于磁珠15的吸附力。同时利用冲洗喷头1331对磁珠15进行冲洗，可以进一步加快磁珠分离的速度。
在一些实施例中，分离容器131的两端均设置有开口，包括上方开口和下方开口。分离容器131中的过滤件132用于将从分离容器131的某一开口(例如，上方开口)进入，从另一开口排出的物质进行过滤。在一些实施例中，过滤件132可以为过滤板，过滤板上开设有过滤孔。过滤板的厚度方向与分离容器131的高度方向平行或近似平行。过滤板沿其厚度方向的横截面轮廓与分离容器131的内部腔体沿其高度方向的横截面轮廓匹配。这里所说的匹配可以是指两者形状相同或近似相同，且尺寸相近，以使得所要过滤的物质无法从过滤件132与分离容器131的内壁之间的缝隙通过。在一些实施例中，过滤件132的中心轴线方向可以与分离容器131的中心轴线方向相同或近似相同。在一些实施例中，磁珠15可以从分离容器131的上方开口处进入分离容器131内部。由于过滤件132的过滤孔的孔径小于磁珠15的直径，因此磁珠15将会被过滤件132截留在分离容器131内部。在一些实施例中，过滤孔的孔径大于生物大分子等物质的直径，以使得作为非纯化目标物的生物大分子(即杂质)和作为纯化目标物的生物大分子与磁珠15分离混入到特定液体中后可以经由过滤孔通过分离容器131的下方开口排出。例如，混合非纯化蛋白的洗杂液可以经由过滤孔从分离容器131的下方开口流出，而磁珠15将会继续堆积在过滤件132上被冲洗。
如图4所示，在一些具体实施例中，分离容器131可以为柱状容器，其两端均设有开口，并且两端的开口相互连通。
在一些实施例中，过滤件132可以设置在靠近分离容器131的下方开口处的位置。例如，在图23和图24所示的实施例中，分离容器131的底部为漏斗状，下方开口的管径小于其余部分的管径。过滤件132可以设置在该漏斗状底部的广口端(下方开口对应漏斗状底部的窄口端)。在一些实施例中，过滤件132可以为筛板。在一些实施例中，筛板可以相对分离容器131拆卸。在一些情况下，可以方便对筛板进行清洗或更换。在另一些情况下，可以取出堆积在筛板上的磁珠15。示例性，筛板可以沿分离容器131的高度方向相对分离容器131向上移动，通过分离容器131的上方开口取出。
在一些实施例中，分离容器131的上方还可以设置有漏斗134。漏斗134可以包括广口端和窄口端，漏斗134的窄口端可以与分离容器131的上方开口连通。如图18至图2所示，在一些情况下，漏斗134的广口端可以用于收容磁性件121(例如，磁棒外套1212)，窄口端可以用于连通分离容器131，以保证磁珠15脱离磁性件121之后可以顺利掉落到分离容器131内。同时漏斗134可以防止磁性件121或磁珠15上残余的培养液滴落到分离容器131外造成污染。在一些实施例中，漏斗134的窄口端的外径可以小于分离容器131的内径，使得漏斗134的窄口端可以嵌入分离容器131的上方开口，以保证漏斗134中的磁珠15和/或液体能够流入到分离容器131中。在一些实施例中，漏斗134的广口端的内径大于磁性件121的外径，以使得吸附有磁珠15的磁性件121可以顺利地从广口端伸入漏斗134内。
在一些实施例中，漏斗134可以与前述实施例中的冲洗组件1330相结合。示例性的，漏斗134的广口端可以设置冲洗喷头1331。在另一示例中，旋转台1332可以设置在漏斗134的广口端并且可以沿漏斗134的广口端转动。在又一示例中，旋转台1332可以设置在漏斗134的广口端并且可以沿漏斗134的广口端转动，旋转台1332上还设置有冲洗喷头1331。
图25-图32示出了另一种分离组件的结构示意图。结合图25至图31所示，在一些实施例中，分离容器131可以包括重力柱1311，重力柱1311的上方开口可以与漏斗134连通。分离组件13可以包括密封塞1361，重力柱1311的下方开口可以通过密封塞1361密封。与重力柱1311连通的排液管路(例如，图21中的排液管路17)的入口设置有穿刺针管1362，穿刺针管1362能够穿过密封塞1361。在一些情况下，通过设置密封塞1361和穿刺针管1362，利用穿刺针管1362穿过密封塞1361来将排液管路和重力柱1311的下方开口密封连接，可以有效避免重力柱1311与排液管路的连通处漏液的情况出现。
在一些实施例中，密封塞1361可以由塑料、橡胶、硅胶等材料制成，以便于穿刺针管1362能够顺利穿透，并且穿刺针管1362拔出后，能够及时恢复形变，从而将重力柱1311的下方开口进行密封。
在一些实施例中，重力柱1311内可以设置有塞板，塞板可以作为过滤件132对重力柱1311中的磁珠进行截留。
在一些实施例中，分离组件13还可以包括：支撑架1363，重力柱1311可放置于支撑架1363内；适配结构，适配结构可以用于将不同的重力柱1311与支撑架1363进行配接。其中，不同的重力柱1311可以是指不同规格的重力柱1311。重力柱1311的规格可以是指重力柱1311的尺寸参数，例如，重力柱1311的直径、容积等。在一些情况下，通过设置适配结构，可以实现不同的重力柱1311与支撑架1363的配接。因此即使重力柱1311的规格不同，也可以采用对应规格的适配结构将重力柱1311与支撑架1363配接，使得支撑架1363能够兼容不同的重力柱1311。
在一些实施例中，支撑架1363可以为中空柱状结构，重力柱1311可以放入支撑架1363的内部。在一些实施例中，如图29所示，支撑架1363可以设有观察窗13631，以方便操作人员从外部观察重力柱1311的情况。
在一些实施例中，适配结构可以包括卡扣1364，卡扣1364可以设于支撑架1363的上方开口，用于限制重力柱1311晃动。
在一些实施例中，卡扣1364可以为环状结构，例如，卡环。卡环可以设置在支撑架1363的上方开口处，重力柱1311可以经由卡环放入到支撑架1363内，当重力柱1311晃动时，卡环可以对重力柱1311起到限制作用。例如，卡环可以与重力柱1311过盈配合，从而完全限制重力柱1311的晃动。
在一些实施例中，适配结构还可以包括压头1365，压头1365可以设于支撑架1363的下方开口，压头1365设有液体通道13651，压头1365的上方包括第一抵接面13652，液体通道13651的上方开口设于第一抵接面13652，液体通道13651的下方开口通过密封塞1361密封；重力柱1311的下方开口端包括与第一抵接面13652适配的第二抵接面1312，第一抵接面13652与第二抵接面1312抵接时，重力柱1311的下方开口伸入液体通道13651中。在本实施例中，当重力柱1311放入支撑架1363内之后，在自身重力作用下，重力柱1311会与压头1365抵接，从而使第一抵接面13652与第二抵接面1312抵接，进而使得重力柱1311的下方开口能够伸入液体通道13651中，再通过密封塞1361和穿刺针管1362能够实现与排液管路的密封连接。此外，由于第一抵接面13652和第二抵接面1312适配，因此当两者抵接之后，能够形成密封的效果，防止漏液。
在一些实施例中，第一抵接面13652和第二抵接面1312可以包括适配的锥状面。仅作为示例，如图29所示，第二抵接面1312可以为外锥状面，即锥状面向外凸出，重力柱1311的下方开口从外锥状面的底部伸出，第一抵接面13652可以为内锥状面，即锥状面向内凹陷，液体通道13651与内锥状面的底部连通。在一些实施例中，第一抵接面13652可以为外锥状面，第二抵接面1312可以为内锥状面。在另一些实施例中，第一抵接面13652和第二抵接面1312可以为平面、波浪面等形状。
在一些实施例中，适配结构与支撑架1363可以是可拆卸连接。例如，卡扣1364可以卡接在支撑架1363的上沿。又例如，压头1365可以通过磁吸连接、螺钉连接等方式与支撑架1363的下沿可拆卸连接。
在一些实施例中，如图28所示，分离组件13还可以包括支撑架卡槽1366，支撑架卡槽1366可以用于放置支撑架1363。支撑架卡槽1366上方具有开口，支撑架1363可以从支撑架卡槽 1366的上方开口放入支撑架卡槽1366内。
在一些实施例中，如图29所示，漏斗134的窄口端可以设置O型圈1367，O型圈1367与重力柱1311的上方开口适配，漏斗134的窄口端可以通过O型圈1367与重力柱1311密封连接。仅作为示例，如图29所示，O型圈1367可以设置在窄口端的外沿，当漏斗134的窄口端伸入重力柱1311的上方开口后，O型圈1367可以与重力柱1311的上方开口的内壁形成过盈配合，从而确保漏斗134与重力柱1311连接的密封性。
在一些实施例中，如图30所示，重力柱1311可以包括不同规格的第一重力柱1313或第二重力柱1314，卡扣1364可以包括第一卡扣13641或第二卡扣13642，压头1365可以包括第一压头13653或第二压头13654；第一卡扣13641、第一压头13643可以与第一重力柱1313适配，第二卡扣13642、第二压头13654可以与第二重力柱1314适配。
在一些实施例中，第一卡扣13641和第二卡扣13642可以不同。仅作为示例，第一卡扣13641和第二卡扣13642的规格不相同。在一些实施例中，第一压头13653和第二压头13654可以不同。仅作为示例，第一压头13653和第二压头13654的规格不相同。图30示例性的示出了支撑架1363分别与第一重力柱1313和第二重力柱1314连接的示意图。其中，由于图30中的第一重力柱1313的直径、容积大于第二重力柱1314的直径和容积，因此需要采用尺寸更大的第一卡扣13641(相较于第二卡扣13642)对第一重力柱1313进行固定。此外，第一重力柱1313的第二抵接面1312直径大于第二重力柱1314的第二抵接面1312直径，因此需要直径更大的第一抵接面13652(相较于第二压头13654的第一抵接面13652)进行配合，因而第一压头13653的直径(相较于第二压头13654)更大。
在本实施例中，由于重力柱1311具有不同的规格，因此当更换不同规格的重力柱1311时，可以使用与该规格重力柱1311适配的卡扣1364和压头1365将重力柱1311用于支撑架1363进行连接。在一些情况下，通过设置不同规格的重力柱1311，能够有效满足对于不同纯化目标的需求。此外，通过配备与重力柱1311规格适配的卡扣1364与压头1365(即适配结构)，能够使支撑架1363兼容不同规格的重力柱1311，降低使用成本。同时，由于支撑架1363可以兼容不同规格的重力柱1311，因此支撑架卡槽1366也可以持续使用，进一步降低耗材成本。
可以理解的是，关于第一重力柱1313和第二重力柱1314的示例仅出于说明目的，并不限制重力柱1311的规格为两种，除第一重力柱1313和第二重力柱1314，还可以包括其他规格的第三重力柱、第四重力柱等。相应的，每种规格的重力柱1311均具备适配的卡扣1364和压头1365(即适配结构)，以便于将不同规格的重力柱1311与支撑架1363进行连接。
在一些实施例中，如图29所示，漏斗134的窄口端设置有压紧法兰1368，压紧法兰1368与卡扣1364配接时，可以将重力柱1311压紧于压头1365上。仅作为示例，在将重力柱1311放入支撑架1363之后，可以通过旋转压紧法兰1368的方式将其与卡扣1364配接，从而实现重力柱1311与支撑架13631的锁紧，同时将重力1311柱压紧在压头1365上。在一些情况下，通过压紧法兰1368和卡扣1364的配合，不仅可以实现重力柱1311与支撑架1363的锁紧，还可以将重力柱1311压紧在压头1365上，有效避免重力柱1311与压头1365脱离导致漏液。
在一些实施例中，除了压紧法兰1368和卡扣1364，还可以通过其他的方式来实现上述效果。在一些实施例中，漏斗134的窄口端可以设置有磁吸件，支撑架1363的上方开口可以设置有磁铁，通过磁吸件与磁铁的磁吸作用可以将重力柱1311与支撑架1363锁紧。
在一些实施例中，结合图29和图30所示，漏斗134可以包括第一漏斗1341或第二漏斗1342，O型圈1367可以包括第一O型圈13671或第二O型圈13672，第一漏斗1341、第一O型圈13671可以与第一重力柱1313适配，第二漏斗1342、第二O型圈13672可以与第二重力柱1314适配。
在一些实施例中，旋转台1332可沿重力柱1311的高度方向运动，以便对重力柱1311进行更换、拆卸或安装。仅作为示例，在纯化设备10工作过程中，漏斗134需要与旋转台1332配合来接收磁珠(如图24及其实施例所描述)，而当需要更换、拆卸重力柱1311时，漏斗134需要连同重力柱1311一起更换、拆卸，而旋转台1332会对漏斗134进行干涉，不便于取出重力柱1311，因此需要控制旋转台1332抬升。
在一些实施例中，结合图1、图26和图28所示，分离组件13还可以包括沿重力柱1311的高度方向设置的第一升降轨道1371，旋转台1332可沿第一升降轨道1371滑动。仅作为示例，分离组件13可以包括第一升降轨道1371、第一升降电机1372和第一升降丝杠1373，第一升降电机1372与第一升降丝杠1373传动连接，第一升降丝杠1373与旋转台1332连接，第一升降电机1372 可以驱动第一升降丝杠1373沿第一升降轨道1371运动，从而带动旋转台1332运动。在一些情况下，通过设置第一升降轨道1371，可以规范旋转台1332的运动路径，提高旋转台1332在运动过程中的稳定性。
在一些实施例中，支撑架1363可沿重力柱1311的高度方向移动。在本实施例中，通过控制支撑架1363的重力柱1311的高度方向运动，可以控制穿刺针管1362从密封塞1361拔出或穿入密封塞1361，以便对重力柱1311进行更换、拆卸或安装。仅作为示例，结合图1、图26和图27所示，在纯化设备10工作过程中，穿刺针管1362需要穿入密封塞1361，将排液管路与重力柱1311连通。由于穿刺针管1362相对纯化设备10固定，因此当需要拆卸、拆卸重力柱1311时，可以控制支撑架1363沿重力柱1311的高度方向运动，使穿刺针管1362从密封塞1361拔出，使得重力柱1311在后续运动过程中避让穿刺针管1362。
在一些实施例中，分离组件13还可以包括沿重力柱1311的高度方向设置的第二升降轨道，支撑架1363可沿第二升降轨道滑动。仅作为示例，结合图1、图28和图28所示，分离组件13可以包括第二升降电机1381，第二升降轨道可以包括支撑架卡槽1366的沿重力柱1311高度方向设置的导向轴13661，支撑架1363可以包括沿重力柱1311高度方向设置的导向条13632，导向条13632与导向轴13661适配，第二升降电机1381能够驱动导向条13632沿导向轴13661运动，从而带动支撑架1363抬升或下降。在一些情况下，通过设置第二升降轨道，可以规范支撑架13631的运动路径，提高支撑架13631在运动过程中的稳定性。在另一示例中，分离组件13可以包括与第二升降电机1381传动连接的第二升降丝杠，第二升降丝杠可以与导向条13632连接，第二驱动电机可以通过驱动第二升降丝杠带动导向条13632沿导向轴13661运动。
在一些实施例中，支撑架1363可沿垂直于重力柱1311的高度方向。当穿刺针管1362从密封塞1361中拔出之后，可以控制支撑架1363从旋转台1332的下方移动到其他位置，以便于对重力柱1311进行更换或拆卸。其中，垂直于重力柱1311的高度方向可以是指垂直于重力柱1311高度方向的平面内的某一方向。
在一些实施例中，结合图1和图28所示，分离组件13还可以包括沿垂直于重力柱1311的高度方向设置的平移轨道1391，支撑架1363可沿平移轨道1391滑动。仅作为示例，分离组件13可以包括平移轨道1391、平移电机1392和平移丝杠1393，平移丝杠1393可以与平移轨道1391适配，且与支撑架卡槽1366连接，平移电机1392可以驱动平移丝杠1393沿平移轨道1391滑动，从而带动支撑架卡槽1366以及设置在支撑架卡槽1366上的支撑架1363滑动。
结合图33和图36所示，在一些实施例中，收集组件14可以包括加液口141和收集容器145。收集容器145可以用于收集混合有纯化目标物(纯化目标物含量达到第二含量阈值)的第二液体。加液口141可以与分离容器131的下方开口连通，将分离容器131中的液体排入收集容器145中。示例性的，加液口141可以通过泵阀组件的排液管路17与分离容器131的下方开口连通。
在一些实施例中，收集组件14还可以包括废液暂存容器146。废液暂存容器146可以用于收集洗杂过程中混合有杂质的第一液体(如PBS洗杂液)，以及洗脱过程中混合有纯化目标物但其含量未达到第二含量阈值的第二液体(如甘氨酸洗脱液)。在一些实施例中，在洗杂处理阶段，加液口141可以位于废液暂存容器146的上方开口处(如图33所示的位置)，用于将分离容器131中的洗杂液排入废液暂存容器146中。在一些实施例中，在洗脱开始阶段，在纯化目标物含量未达到第二含量阈值之前，加液口141依旧可以位于废液暂存容器146的上方开口处，用于将洗脱液排入废液暂存容器146中。直到洗脱液中的纯化目标物含量达到第二含量阈值时，加液口141可以移动到收集容器145的上方开口处，用于将洗脱液存储在收集容器145中。
在一些具体实施例中，如图33所示，废液暂存容器146的内径可以大于收集容器145的内径。在一些应用场景中，由于废液暂存容器146不仅需要存储洗杂液，还可能会用于存储一部分洗脱液。示例性的，当洗脱液中的纯化目标物含量未达到第二含量阈值时，洗脱液可能会被排放到废液暂存容器146当中。基于上述原因，废液暂存容器146的容积可以大于收集容器145的容积。在一些实施例中，废液暂存容器146的底部可以设置有排出管路，排出管路可以与外部的容积较大的容器连通。废液暂存容器146中的废液可以经由排出管路排入到该容器中，防止废液暂存容器146内的液体溢出。
在一些实施例中，收集组件14还可以包括额外加液口142。额外加液口142可以用于排出其他类型的液体，示例性的，其他类型的液体可以包括但不限于中和液、纯水、氢氧化钠溶液等。在一些实施例中，中和液可以包括pH值偏高的缓冲液。在一些应用场景中，当纯化目标物的类型为抗体时，中和液可以用于将收集容器145中的洗脱液中的酸性环境中和成pH中性，避免收集容 器145中的抗体长期处于酸性环境受到破坏。需要说明的是，额外加液口142可以不是必须的，中和液可以通过其他的通道排出。例如，加液口141既可以用于排出分离容器131中的液体，也可以排出中和液。
如图33所示，在一些实施例中，收集组件14还可以包括沿废液暂存容器146上方至收集容器145上方方向设置的第四轨道144。加液口141可以沿第四轨道144滑动。在一些实施例中，在一些情况下，通过设置第四轨道144，可以规范加液口141的移动路径，提高加液口141运动时的稳定性，保证加液口141排出的液体进入收集容器145或废液暂存容器146当中。
如图33所示，在一些实施例中，收集组件14还可以包括底座147。在沿底座147的长度方向上设置有多个孔位。多个收集容器145可以放置在对应的孔位中进行固定，以便于对多个收集容器145批量操作。在一些实施例中，第四轨道144方向可以与从底座147其中一个收集容器145的上方开口至另一个收集容器145的上方开口的方向相同或近似相同，以便于加液口141可以沿第四轨道144从其中一个收集容器145的上方开口移动至另一个收集容器145的上方开口。在一些应用场景中，当其中一个收集容器145中的液面达到一定高度(例如，第三液面高度阈值)时，加液口141可以沿第四轨道144移动到相邻的收集容器145的上方开口排出液体。在一些实施例中，废液暂存容器146可以与底座147相邻设置，以减小加液口141从废液暂存容器146移动至收集容器145所需的距离。
在一些实施例中，如图35所示，收集组件14可以包括底座147和收集容器支架148，收集容器支架148可以用于放置收集容器145，收集容器支架148可以安装于底座147。仅作为示例，收集容器支架148可以包括多个放置孔位，每个放置孔位可以放置一个收集容器145，以便进行批量化操作。
在一些实施例中，结合图34和图35所示，收集容器145可以包括不同规格的第一收集容器1451或第二收集容器1452，收集容器支架148可以包括第一收集容器支架1481或第二收集容器支架1482，第一收集容器支架1481可以用于放置第一收集容器1451，第二收集容器支架1482可以用于放置第二收集容器1452，第一收集容器支架1481的尺寸与第二收集容器支架1482的尺寸相同。其中，第一收集容器支架1481的尺寸与第二收集容器支架1482的尺寸相同可以是指第一收集容器支架1481的长度、高度、宽度等尺寸与第二收集容器支架1482相同。由于第一收集容器支架1481与第二收集容器支架1482尺寸相同，因此第一收集容器支架1481和第二收集容器支架1482的长度方向可以是同一方向，通过图34所示的箭头X1来表示。第一收集容器支架1481的高度方向和第二收集容器支架1482的高度方向可以是同一方向，通过图34所示的箭头Y1来表示。
在一些实施例中，底座147上可以安装一个或多个第一收集容器支架1481或者第二收集容器支架1482。在一些实施例中，收集容器支架148上可以放置一个或多个同样规格的收集容器145。
仅作为示例，第一收集容器1451的容积为50ml，第二收集容器1452的容积为125ml，且第一收集容器1451的外径小于第二收集容器1452的外径。第一收集容器支架1481包括多个第一放置孔位，第二收集容器支架1482可以包括多个第二放置孔位，第一放置孔位与第一收集容器1451适配，第二放置孔位与第二收集容器1452适配。在一些情况下，相同尺寸的收集容器支架148可以兼容不同规格的收集容器145，从而能够安装于同一个底座147上，不仅更方便批量化操作，还能够有效节省设备成本。
结合图33和图36所示，在一些实施例中，收集组件14还可以包括与加液口141连接的加液头143。加液头143可以用于固定加液口141，提高加液口141在工作过程中的稳定性。在一些实施例中，加液头143可以包括依次固定连接的第一板体1431、第二板体1432以及第三板体1433。其中，第一板体1431与第四轨道144滑动连接。第二板体1432相对第一板体1431呈一定角度(例如，90度)设置。第三板体1433与第一板体1431平行或近似平行(例如，两个板体之间的夹角小于5度)。加液口141设置在第三板体1433上。在本实施例中，当由第一板体1431、第二板体1432以及第三板体1433组成的加液头143沿第四轨道144滑动时，可以带动设置在第三板体1433上的加液口141滑动。
在一些实施例中，检测组件可以包括第一检测组件161。第一检测组件161可以是指检测容器中液位(即液面位置)是否达到设定液位的仪器或设备。第一检测组件161可以包括液位传感器，用于检测分离容器131中的液面位置是否达到设定的液位。液面位置可以是指分离容器131中的液体最高点的位置。根据本说明书其他实施例的描述，在纯化过程中，需要向分离容器131中加入第一液体对磁珠15进行洗杂，以及在洗杂完成后加入第二液体对磁珠15进行洗脱。在一些实施 例中，为了保证杂质和纯化目标物更快脱离磁珠15并与液体混合，需要让第一液体或第二液体与磁珠15充分接触，同时也要避免分离容器131中的液体过多发生液体溢出的情况发生。因此需要对分离容器131内的液面位置进行检测并根据检测结果进行调整，使其保持在合适的范围内。在一些情况下，通过在适当位置设置液位传感器，可以检测分离容器131中的液面位置是否达到设定的位置，进而可以根据检测结果控制加液量(例如，可以通过控制冲洗喷头1331喷出的液体量来实现)和排液量(例如，可以通过控制加液口141排出的液体量来实现)。需要说明的是，第一检测组件161可以不是必须的。在一些实施例中，分离容器131内的液体体积可以通过冲洗组件1330(如冲洗喷头1331)排出的液体体积和从分离容器131内排出的液体体积来确定。示例性的，分离容器131内的液体体积可以等于冲洗组件1330排出的液体体积与从分离容器131内排出的液体体积的差值。
在一些实施例中，第一检测组件161可以包括第一液位传感器1611。第一液位传感器1611可以设置在分离容器131的第一位置，用于检测液面位置与第一位置之间的关系。在一些实施例中，第一位置可以为分离容器131的内部腔体的顶部(即分离容器131的上方开口处)至底部(例如，过滤件132上表面所在的位置)之间的任意位置。示例性的，第一位置可以为分离容器131的内部腔体的高度的1/3处。当液位传感器检测到液面位置未达到第一位置时，表明分离容器131中的液位高度过低，液体过少。
在一些实施例中，第一检测组件161还可以包括第二液位传感器1612。第二液位传感器1612可以设置在分离容器131的第二位置。第一位置的高度可以高于第二位置的高度。在本实施例中，第一液位传感器1611可以检测液面位置是否达到第一位置，第二液位传感器1612可以检测液面位置是否达到第二位置。示例性的，设置在第一位置(例如，第一位置为分离容器131的内部腔体的高度的4/5处)的第一液位传感器1611可以用于检测最高液面位置(或称为第一液面阈值)。当液面位置达到第一位置时，表明分离容器131内的液体过多。设置在第二位置(例如，第二位置为分离容器131的内部腔体的高度的1/3处)的第二液位传感器1612可以用于检测最低液面位置(或称为第二液面阈值)。当液面位置未达到第二位置时，表明分离容器131内的液体过少。在一些实施例中，第一液位传感器1611和第二液位传感器1612可以是相同的液位传感器，也可以是不同的液位传感器。
在一些实施例中，液位传感器的类型可以包括但不限于法兰压差液位传感器、浮球式液位传感器、磁性液位传感器、投入式液位传感器、电容式液位传感器、电动浮球液位传感器等。在一些具体实施例中，液位传感器可以为电容式液位传感器，以便于在分离容器131外部对分离容器131内的液位进行检测。
如图23和图24所示，在一些实施例中，纯化设备10的检测组件还可以包括第二检测组件162。第二检测组件162可以用于检测加入到分离容器131中的第一液体的体积。
在一些实施例中，在将磁珠与纯化目标物分离之前，需要检测杂质是否与磁珠分离完毕。当确定磁珠上的杂质移除完毕后，可以利用第二液体对磁珠进行冲洗，以保证磁珠上没有残留的杂质混合在第二液体中或者杂质的含量可以忽略不计。
在一些实施例中，第二检测组件162可以包括第一流量传感器，第一流量传感器可以检测加入到分离容器131中的第一液体的体积。在一些具体实施例中，第一流量传感器可以为第一流量阀，第一流量阀可以设置在冲洗组件(如冲洗组件1330)的冲洗喷头1331上，用来检测冲洗喷头1331喷出的第一液体的流量。当喷出的第一液体的体积达到设定的体积阈值(可称为第一体积阈值)时，表明洗杂处理完成。
在一些替代性实施例中，第二检测组件162可以检测经过滤件132的过滤孔通过分离容器131的下方开口排出的第一液体的体积。当第一液体的体积达到第二体积阈值时，可以表明洗杂已经完成。在一些实施例中，第二体积阈值可以等于第一体积阈值。
在一些实施例中，从分离容器131的下方开口排出的第一液体的体积也可以通过第二检测组件进行检测。在一些实施例中，第二检测组件162可以包括第二流量传感器，第二流量传感器可以检测分离容器131的下方开口排出的第一液体的体积。在一些具体实施例中，第二流量传感器可以为第二流量阀，第二流量阀可以设置在与分离容器131的下方开口连通的排液管路17上，用来检测从分离容器131的下方开口排出的第一液体的流量。
在一些实施例中，检测组件还可以包括第三检测组件163。第三检测组件163可以是指检测液体中某种物质含量的仪器或设备。第三检测组件163可以用于检测与分离容器131连通的排液管路17中第一液体的杂质含量和/或第二液体的纯化目标物含量。示例性的，在洗杂处理过程中，加入第一液体将磁珠15上的杂质移除，磁珠15上的杂质会混合在第一液体中经由过滤件132通过 分离容器131的下方开口排出。可以理解的是，随着洗杂的不断进行，磁珠15上的杂质会越来越少，混合在第一液体中的杂质含量会逐渐降低并趋于稳定。在一些实施例中，当洗杂液中的杂质含量达到第一含量阈值(该阈值可以称为基线)并维持检测时间阈值不变(即没有变化或者几乎没有变化)时，表明磁珠15上的杂质已完全与磁珠15分离或者残余的杂质可以忽略不计，即洗杂完成。
在另一示例中，在洗脱处理过程中，加入第二液体将纯化目标物(例如，目标蛋白)与磁珠15分离，纯化目标物会混合在第二液体中。在一些实施例中，当洗脱液(即第二液体)中的纯化目标物含量逐渐上升并达到第二含量阈值时，表明洗脱已经达到纯化要求，可以对第二液体进行收集，因此可以将其收集至收集容器145中。可以理解的是，随着洗脱的不断进行，第二液体中的纯化目标物含量会逐渐上升至最高点然后下降。在一些实施例中，当第二液体中的纯化目标物含量达到第三含量阈值且处于下降趋势时，表明从磁珠15上分离的纯化目标物较少，可以认为磁珠15上残留的纯化目标物较少或完全没有残留。因此可以停止向分离容器131中加入第二液体并结束对第二液体的收集。第三含量阈值可以是指满足收集要求的第二液体中的纯化目标物含量的最低值。关于确定是否停止收集第二液体的更多细节可以参见图39的描述，此处不再赘述。
如图1、2、4、6、10、14、18所示，在一些实施例中，第三检测组件163可以包括与排液管路17连通的含量检测传感器。在一些实施例中，含量检测传感器的类型与所要检测的杂质类型或者纯化目标物类型有关。为了方便描述，本说明书将以非纯化蛋白(即杂质)以及纯化蛋白为例进行说明。在一些实施例中，含量检测传感器可以包括紫外线吸收检测器。紫外吸收检测器可以根据所要检测的物质对紫外光吸收强弱与所要检测的物质浓度成正比的原理对液体中的特定物质的含量进行检测。示例性的，紫外吸收检测器可以检测第一液体在紫外光吸收峰为280nm处的紫外光吸收值(或称为光密度值)，进而根据紫外光吸收值确定液体中非纯化蛋白的含量。示例性的，紫外吸收检测器可以检测第二液体在特定紫外光吸收峰(例如，紫外光吸收峰为280nm)处的紫外光吸收值，从而根据紫外光吸收值确定纯化蛋白的含量。
在另一些实施例中，在检测第一液体中的杂质含量或者第二液体中的纯化目标物含量时，第三检测组件163可以不是必须的，可以通过其他检测组件进行检测。例如，废液暂存容器146上设置有特定的含量检测传感器，可以用于对废液暂存容器146中的纯化目标物含量进行检测。再例如，收集容器145上设置有特定的含量检测传感器，用于对收集容器145中的纯化目标物含量进行检测。
如图33和图36所示，在一些实施例中，检测组件还可以包括第四检测组件164。第四检测组件164可以是指检测容器中液位(即液面位置)是否达到设定液位的仪器或设备。第四检测组件164可以用于检测废液暂存容器146和/或收集容器145中的液面位置是否达到设定的位置。在一些实施例中，第四检测组件164可以设置在收集组件14的加液头143上。示例性的，第四检测组件164可以设置在第三板体1433上，并且被配置为当加液口141位于收集容器145的上方开口时，第四检测组件164也位于收集容器145的上方开口。当加液头143沿着第四轨道144移动时，可以带动第四检测组件164移动到废液暂存容器146或收集容器145的上方开口，对其液面位置进行检测。
如图33所示，在一些实施例中，第四检测组件164可以用于检测收集容器145中的液面位置是否达到设定的第三液面阈值。在一些情况下，当其中一个收集容器145中的液体达到第三液面阈值时，加液口141可以停止加液，防止收集容器145中的液体溢出。在一些实施例中，第四检测组件164可以用于检测废液暂存容器146中的液面位置是否达到第四液面阈值。当废液暂存容器146中的液体达到第四液面阈值时，可以将液体通过设置在其底部的排出管路排到外部的容积较大的容器中，避免废液溢出。
在一些实施例中，第四检测组件164可以包括如前述实施例所描述的一种或多种液位传感器。在一些具体实施例中，第四检测组件164可以包括超声波液面传感器。
在一些实施例中，纯化设备10可以包括第五检测组件165，第五检测组件165可以包括第一光电传感器1651，第一光电传感器1651可以用于检测支撑架1363内是否安装有第一重力柱1313或者第二重力柱1314。仅作为示例，结合图31和图32所示，第五检测组件165包括两个第一光电传感器1651、两个弹簧销1652。两个第一光电传感器1651对称设置在保持架卡槽1366下方。保持架卡槽1366与第一光电传感器1651对应的位置设置有两个安装孔1653，两个安装孔1653的中心轴线均与重力柱1311的高度方向平行。两个安装孔1653内分别设置有一个弹簧1654。为方便描述，两个弹簧销1652中的其中一个可以称为第一弹簧销，另一个可以称为第二弹簧销。两个安装孔1653中的一个安装孔1653可以称为第一安装孔，另一个可以称为第二安装孔。两个弹簧1654中的其中一个弹簧1654可以称为第一弹簧，另一个弹簧1654可以称为第二弹簧。第一弹簧销穿过第一 弹簧且第一弹簧的上端与第一弹簧销抵接，第二弹簧销穿过第二弹簧且第二弹簧的上端与第二弹簧销抵接。弹簧销1652通过弹簧1654可实现沿安装孔1653的中心轴线方向运动。
支撑架1363的导向条13632的数量为两个，两个导向条13632的下端分别与两个安装孔1653对应，其中一个导向条13632的下端面设置有凹槽。为了方便描述，两个导向条13632中的一个可以称为第一导向条，另一个可以称为第二导向条。第一导向条的下端设置有凹槽。导向条13632的下端与安装孔1653对应可以是指导向条13632的下端与安装孔1653对齐，使得导向条13632的下端能抵接弹簧销1652。在一些实施例中，当第一重力柱1313与支撑架1363配接并放入支撑架卡槽1366内时，第一导向条的下端可以与第一安装孔对齐，第二导向条的下端可以与第二安装孔对齐。在一些实施例中，当第二重力柱1314与支撑架1363配接并放入支撑架卡槽1366内时，第一导向条的下端可以与第二安装孔对齐，第二导向条的下端可以与第一安装孔对齐。
当支撑架卡槽1366中未安装支撑架1363时，弹簧销1652的上端均伸出安装孔1653。当支撑架1363以及第一重力柱1313放入支撑架卡槽1366时，第一导向条在支撑架1363以及第一重力柱1313的重力作用下可以抵接第一弹簧销的上端并推动第一弹簧销挤压第一弹簧，促使第一弹簧压缩，进而使第一弹簧销的下端与对应的第一光电传感器1651接触。而当支撑架1363以及第二重力柱1314放入支撑架卡槽1366时，第二导向条在支撑架1363以及第二重力柱1314的重力作用下可以抵接第二弹簧销的上端并推动第二弹簧销挤压第二弹簧，促使第二弹簧压缩，进而使第二弹簧销的下端与对应的第一光电传感器1651接触。所以，当第一弹簧销对应的第一光电传感器1651产生光电传感信号时，表明支撑架卡槽1366内放置有支撑架1363，且支撑架1363内安装有第一重力柱1313。当第二弹簧销对应的第一光电传感器1651产生光电传感信号时，表明支撑架卡槽1366内放置有支撑架1363，且支撑架1363内安装有第二重力柱1314。当两个第一光电传感器1651均未产生光电传感信号时，支撑架卡槽1366内未放置支撑架1363和重力柱1311。
在一些实施例中，结合图34和图35所示，纯化设备10还可以包括第六检测组件166，第六检测组件166可以用于检测底座147上是否安装有第一收集容器支架1481或者第二收集容器支架1482。
在一些实施例中，第六检测设备166可以包括第二光电传感器1661，第二光电传感器1661可以用于检测底座147上是否安装有第一收集容器支架1481或者第二收集容器支架1482。
仅作为示例，第六检测组件166可以包括第一光电感应片1662、第二光电感应片1663和两个第二光电传感器1661，第一收集容器支架1481的长度方向的至少一端设置有第一光电感应片1662，第一光电感应片1662设于第一收集容器支架1481的高度方向上的第一高度位置。第二收集容器支架1482的长度方向的至少一端设置有第二光电感应片1663，第二光电感应片1663设于第二收集容器支架1482的高度方向上的第二高度位置。第一高度位置和第二高度位置不同。
底座147的长度方向上的至少一端设置有两个第二光电传感器1661，两个第二光电传感器1661分别位于底座147高度方向上的第三高度位置和第四高度位置。底座147的长度方向可以通过图35中的箭头X2来表示。底座147的高度方向可以通过图35中的箭头Y2来表示。底座147的长度方向与第一收集容器支架1481的长度方向平行。当第一收集容器支架1481安装于底座147上时，第一高度位置与第三高度位置相同，因此位于第一高度位置的第一光电感应片1662能够被位于第三高度位置的第二光电传感器1661检测到，该第二光电传感器1661可以产生传感信号。当第二收集容器支架1482安装于底座147上时，位于第二高度位置的第二光电感应片1663能够被位于第四高度位置的第二光电传感器1661检测到，该第二光电传感器1661可以产生传感信号。基于上述内容，当位于第三高度位置的第二光电传感器1661产生光电传感信号时，表明底座147上安装的是第一收集容器支架1481。当位于第四高度位置的第二光电传感器1661产生光电传感信号时，表明底座147上安装的是第二收集容器支架1482。当两个第二光电传感器1661均未产生光电传感信号时，表明底座147上没有安装收集容器支架148或者收集容器支架148安装不到位。
在一些实施例中，第一收集容器支架1481的长度方向的两端均设置有第一光电感应片1662，第二收集容器支架1482的长度方向的两端均设置有第二光电感应片1663。如此设置之后，第一收集容器支架1481和第二收集容器支架1482两端的光电感应片均可以被第二光电传感器1661检测到，无需按照特定的方向安装第一收集容器支架1481和第二收集容器支架1482，降低安装难度。
在一些实施例中，第六检测设备166可以包括设置在底座147上的重力检测传感器，通过重力检测传感器可以确定底座147上是否安装有第一收集容器支架1481或者第二收集容器支架1482。仅作为示例，第一收集容器支架1481的自身重量与第二收集容器支架1482的自身重量相同，第二收集容器1452的重量大于第一收集容器1451的重量。当第一收集容器支架1481和第二收集 容器支架1482放置相同数量的第一收集容器1451和第二收集容器1452时，由于，所以第二收集容器支架1482的重量相较第一收集容器支架1481更重，通过底座147上的重力传感器的检测结果即可确定。
在一些实施例中，纯化设备10的一个或多个组件可以通过手动操作的方式执行相应功能。示例性的，结合图2所示，可以手动控制震荡平台111对混匀容器112进行震荡。在另一示例中，结合图16所示，可以手动控制磁性件121伸入混匀容器112内吸附磁珠15。在又一示例中，结合图33所示，可以手动控制加液头143和加液口141沿着第四轨道144滑动，从而移动到收集容器145或者废液暂存容器146的上方开口。
在一些实施例中，纯化设备10的一个或多个组件可以由其他组件进行控制以执行相应的功能。如图1所示，在一些实施例中，纯化设备10还可以包括控制组件18，控制组件18可以用于控制前述实施例中的一个或多个组件执行相应的功能。示例性的，控制组件18可以控制混合组件11进行混匀操作，以使混匀容器112中的磁珠15与纯化目标物充分结合。在另一示例中，控制组件18可以控制转移组件12中的磁性件121从混匀容器112内部运动至分离容器131的上方开口。
在一些实施例中，控制组件18可以包括控制单元181和驱动单元182。控制单元181可以用于接收工作指令，工作指令可以由操作人员输入，用于指示控制组件18驱动一个或多个组件执行相应的功能。在一些实施例中，控制单元181可以根据工作指令生成对应的指令，并将指令发送至驱动单元182。在一些实施例中，驱动单元182可以与纯化设备10的一个或多个组件驱动连接。驱动单元182可以接收来自控制单元181的指令，并根据指令驱动对应的组件执行特定功能。以下将结合纯化设备10的一个或多个组件对控制单元181和驱动单元182进行进一步说明。
如图1和图2所示，在一些实施例中，控制单元181可以根据工作指令生成第一指令并发送至驱动单元182。第一指令与混合组件11的震荡平台111对应，可以指示震荡平台111在设定的工作时间内，按照设定转速对混匀容器112进行旋转震荡。在一些实施例中，驱动单元182接收到第一指令后，可以驱动震荡平台111进行工作。例如，驱动单元182可以控制震荡平台111的电机(例如，图13中的震荡驱动电机113)按照第一指令的指示驱动曲轴(例如，图13中的曲轴114)运动，进而带动震荡平台111旋转。
结合图1、图2和图7所示，在一些实施例中，控制单元181可以根据工作指令生成第二指令并发送至驱动单元182。第二指令与转移组件12以及磁珠脱离组件133对应，可以指示转移组件12的磁性件121(例如，磁棒1211和磁棒外套1212)移动到分离容器131的上方开口处，并指示磁珠脱离组件133将磁珠15与磁性件121分离。在一些实施例中，驱动单元182接收到第二指令后，可以控制磁性件121以及磁珠脱离组件133按照第一指令的指示工作。示例性的，磁性件121可以包括磁棒1211和磁棒外套1212，磁棒1211和磁棒外套1212分别通过第一滑动件和第二滑动件与第一轨道123和第二轨道124滑动连接。驱动单元182可以驱动第一滑动件和第二滑动件运动，从而带动磁棒1211和磁棒外套1212沿对应的轨道运动至图18至图21所示的位置，使磁珠15在重力作用下与磁棒外套1212分离。
结合图1、图2、图6和图10所示，在一些实施例中，控制单元181可以根据工作指令生成第三指令并发送至驱动单元182。第三指令与冲洗组件1330对应，可以指示冲洗喷头1331按照一定的速率喷出特定的液体。驱动单元182接收到第三指令后，可以控制冲洗喷头1331按照第三指令的指示进行工作。示例性的，驱动单元182可以驱动与冲洗喷头1331连通的泵阀组件工作，通过冲洗喷头1331喷出第一液体对磁珠15进行冲洗。
在一些实施例中，控制单元181可以根据工作指令生成第四指令并发送至驱动单元182。第四指令与冲洗组件1330对应，可以指示冲洗喷头1331按照一定的速率喷出特定的液体。驱动单元182接收到第四指令后，可以控制冲洗喷头1331按照第四指令的指示进行工作。示例性的，驱动单元182可以驱动与冲洗喷头1331连通的泵阀组件工作，通过冲洗喷头1331喷出第二液体对磁珠15进行冲洗。
结合图1、图4、和图33所示，在一些实施例中，含量检测传感器可以根据与分离容器连通的排液管路中液体中的特定物质的含量生成检测信号。控制组件18还可以用于根据检测信号控制驱动单元182驱动收集组件14收集分离容器131内的液体。
在一些实施例中，含量检测传感器可以根据与分离容器连通的排液管路中第一液体中的杂质含量生成第一含量检测信号和/或第二液体中的纯化目标物含量生成第二含量检测信号。控制单元181可以基于第二含量检测信号生成第五指令并发送至驱动单元182。第五指令与收集组件14对应，可以指示收集组件14收集来自分离容器131内的第二液体。驱动单元182接收到第五指令后，可 以控制收集组件14按照第五指令的指示进行工作。示例性的，结合图33所示，含量检测传感器检测到与分离容器131连通的排液管路17中第二液体中的纯化目标物含量达到第二含量阈值。因此控制单元181可以生成第五指令，第五指令可以指示收集组件14对第二液体进行收集。驱动单元182在接收到该指令后，可以驱动收集组件14的加液头143沿第四轨道144移动至收集容器145的上方开口，并驱动加液口141排出第二液体至收集容器145。
在一些实施例中，纯化设备10还可以包括上位机，上位机可以是指能够发出操控命令(例如，工作指令)的计算机，如电脑、平板等。上位机可以与控制组件18连接/通讯，通过将工作指令发送给控制单元181，指示驱动单元182驱动相应的组件进行工作。在一些实施例中，上位机可以被手动操控，例如，操作人员可以手动操作上位机中的上位机软件发出工作指令。在另一些实施例中，上位机可以通过控制程序或软件对纯化设备10进行控制。在一些实施例中，上位机可以包括显示装置，例如显示屏。显示屏可以用于将一个或多个检测组件的检测结果以特定的形式(例如，数字、图像等)呈现。在一些实施例中，如图3所示，纯化设备10还可以包括交换机19，交换机19可以用于实现上位机可以与控制组件18连接/通讯。
需要说明的是，本说明书所描述的通过控制组件18对纯化设备10的一个或多个组件进行控制的实施例仅出于说明目的，并不旨在限制控制组件18所能控制的组件具体类型。例如，控制组件18还可以接收第四检测组件164发送的收集容器145中液体的液面位置达到第三液面阈值的检测信号。控制单元181可以基于该检测信号生成第六指令，该指令可以指示加液口141从当前收集容器145的上方移动至另一个收集容器145的上方。驱动单元182接收到该指令后，可以驱动加液头143沿第四轨道144进行移动，从而带动加液口141移动到相应的位置。
在一些实施例中，为了提高纯化效率，可以将多个前述实施例中所描述的纯化设备10进行并联，组成新的第一纯化设备组100。例如，在图37所示的实施例中，第一纯化设备组100由4台纯化设备10并联组成。该第一纯化设备组100的多个纯化设备10之间相互独立，在纯化过程中互不影响。在一些实施例中，第一纯化设备组100的一个或多个纯化设备10可以由同一上位机进行控制。在另一些实施例中，第一纯化设备组100的一个或多个纯化设备10可以由不同的上位机进行控制。需要说明的是，本实施例图37中所示的第一纯化设备组100包括4台纯化设备10，并非表示本发明仅仅局限于扩展到4台，可以扩展的台数不作限定。在一些实施例中，第一纯化设备组100的每个纯化设备10可以完全相同。
在一些实施例中，除了将多台纯化设备10并联来提高纯化效率之外，在一些实施例中，还可以提高单台纯化设备10的组件数量来实现。如图38所示，在一些实施例中，第二纯化设备组200可以包括与本说明书其他实施例中的纯化设备10相同的组件。例如，第二纯化设备组200的震荡平台111、混匀容器112、分离组件13(例如，分离容器131)、收集组件14、控制组件18、检测组件(例如，第三检测组件163)、磁珠脱离组件133、转移组件12(例如，磁性件121)可以与纯化设备10的震荡平台111、混匀容器112、分离组件13(例如，分离容器131)、收集组件14、控制组件18、检测组件(例如，第三检测组件163)、磁珠脱离组件133、转移组件12(例如，磁性件121)相同或相似。不同之处在于第二纯化设备组200的震荡平台111可以固定两个混匀容器112，以及设置有两个分离组件13、两个收集组件14、两个转移组件12以及两个检测组件。第二纯化设备组200的组件均可以通过控制组件18独立进行控制，在工作时互不影响。在一些情况下，通过提高组件数量，提高了第二纯化设备组200纯化效率，还可以使第二纯化设备组200结构更加紧凑，体积更加小巧。本实施例图38中所示扩展到2通道(即组件数量为2)，并非表明本发明仅仅局限于扩展到2通道，可以扩展的通道数不作限定。
图39是根据本说明书一些实施例所示的纯化方法的示例性流程图。如图39所示，本说明书还提供一种基于前述一个或多个实施例所描述的纯化设备10，对培养液中的生物大分子进行纯化的方法，该方法3900可以包括以下步骤：
步骤3910、控制混合组件11进行混匀操作。
在一些实施例中，控制混合组件11进行混匀操作包括：首先将一定量的培养液和一定量的磁珠15放入混匀容器112中，然后将混匀容器112固定于震荡平台111并控制震荡平台111对混匀容器112进行震荡，使磁珠15与培养液中的纯化目标物结合。
步骤3920、控制转移组件12中的磁性件121将磁珠15转移至分离容器131的上方开口。
在一些实施例中，控制转移组件12中的磁性件121将磁珠15转移至分离容器131的上方开口包括：将磁性件121的一部分伸入到混匀容器112的液面以下，利用磁性件121的吸附力使磁珠15富集在磁性件121的表面。然后将磁性件121从混匀容器112中取出并移动至分离容器131 的上方开口处。
步骤3930、控制磁珠脱离组件133将磁珠15从磁性件121上脱离，使磁珠15进入分离容器131并堆积于过滤件132上。
步骤3940、控制冲洗组件1330向分离容器131内加入第一液体。通过第一液体冲洗磁珠15来移除未与磁珠15结合的杂质。
步骤3950、控制冲洗组件1330向分离容器131内加入第二液体。通过第二液体冲洗磁珠15使得与磁珠15结合的纯化目标物与磁珠15分离。
步骤3960、控制收集组件14收集来自分离容器131的第一液体和/或第二液体。在一些实施例中，可以通过与分离容器131的下方开口连通的收集组件14收集混合有纯化目标物的第二液体。在一些实施例中，可以控制收集组件14收集来自分离容器131的第一液体。在一些实施例中，可以通过与分离容器131的下方开口连通的收集组件14收集混合有杂质的第一液体。
在一些实施例中，磁性件121可以包括磁棒1211。在将磁棒1211转移至分离容器131的上方开口处的过程中，可以控制磁棒1211沿第一轨道123和第二轨道124滑动，以规范磁棒1211的运动路径，提高运动过程中的稳定性。
在一些实施例中，当磁性件121位于分离容器131的上方开口处时，可以通过设置在分离容器131的上方开口处的磁珠脱离组件133的冲洗组件1330对磁珠15进行冲洗来将磁珠15与磁性件121分离。在一些实施例中，还可以通过减小磁性件121的磁力降低磁性件121对磁珠15的吸附力，使磁珠15在重力作用下与磁性件121分离。例如，可以控制磁棒1211和磁棒外套1212分别移动至图18至图21所示的位置，减小磁棒1211对磁棒外套1212底部的磁珠15的吸附力，使磁珠15在重力作用下脱离。关于通过减小磁性件121的磁力降低磁性件121对磁珠15的吸附力的更多细节，可以参见本说明书其他实施例的描述，此处不再赘述。
在一些实施例中，在洗杂过程中，可以通过第三检测组件163检测与分离容器131连通的排液管路17中的第一液体中的杂质含量。当第一液体中的杂质含量达到第一含量阈值且维持检测时间阈值不变(即没有发生变化或者几乎没有变化)时，表明洗杂完成，可以停止加入第一液体。
在一些实施例中，在洗脱过程中，可以通过第三检测组件163检测与分离容器131连通的排液管路17中第二液体中的纯化目标物含量。当第二液体中的纯化目标物含量达到第二含量阈值时，表明第二液体中的纯化目标物含量满足收集要求，可以对第二液体进行收集。当纯化目标物含量达到第三含量阈值时，表明从磁珠上分离的纯化目标物较少，可以认为磁珠15上残留的纯化目标物较少或完全没有残留，因此可以停止向分离容器131中加入第二液体并结束对第二液体的收集。在一些实施例中，第三含量阈值可以超过第二含量阈值。当第三含量阈值超过第二含量阈值时，若纯化目标物含量达到第三含量阈值，且第二液体中的纯化目标物含量处于下降趋势(例如，可以基于若干个连续的时刻对应的纯化目标物含量数值(如10个数值)，确定目前的纯化目标物含量变化趋势)，则表明磁珠15上残留的纯化目标物较少或完全没有残留，因此可以停止向分离容器131中加入第二液体并结束对第二液体的收集。若纯化目标物含量不处于下降趋势，则可以继续收集第二液体。在一些实施例中，第三含量阈值可以小于第二含量阈值。当第三含量阈值小于第二含量阈值时，若纯化目标物含量达到第三含量阈值，则可以直接确定停止向分离容器131中加入第二液体并结束对第二液体的收集。在一些实施例中，当停止向分离容器131中加入第二液体并结束对第二液体的收集时，可以视为纯化过程结束。
在一些实施例中，当停止向分离容器131中加入第二液体并结束对第二液体的收集后，还可以对纯化设备10的一个或多个组件、管路以及磁珠15进行清洗、消毒。在一些具体实施例中，可以控制冲洗组件1330(例如，冲洗喷头1331)向分离容器131中加入其他液体(包括但不限于PBS洗杂液、纯水、氢氧化钠溶液)。例如，加入PBS洗杂液、纯水或氢氧化钠溶液等对分离容器131内部、过滤件132、排液管路17以及磁珠15进行清洗、消毒。又例如，将磁棒外套1212和漏斗134等部件浸泡在氢氧化钠溶液中进行消毒。
在一些实施例中，前述一个或多个实施例所描述的纯化方法可以通过控制组件18来实现。在一些实施例中，控制关于控制组件18如何控制一个或多个组件的相关细节可以参见图37相关的实施例，此处不再赘述。
本说明书实施例的培养装置可能带来的有益效果包括但不限于：(1)通过在分离容器中设置过滤件，利用过滤件将磁珠与冲洗磁珠后的液体进行分离，以便于对混合有纯化目标物的液体进行收集，有效提高了纯化效率；(2)通过设置第一轨道(对应于磁棒)、第二轨道(对应于磁棒外套)、第三轨道(对应于磁棒外套和磁棒)、第四轨道(对应于加液头)，规范了对应的部件的运 动路径，提高其运动过程中的稳定性；(3)通过在分离容器的上方设置冲洗喷头，利用冲洗喷头喷出的液体对磁珠进行冲洗，可以加快磁珠转移效率，从而提高纯化效率；(4)通过在分离容器的上方设置绕分离容器的中心轴线旋转的旋转台，并且在旋转台上设置冲洗喷头，利用旋转台带动冲洗喷头对磁性件的侧壁进行全方位冲洗，加快了磁珠转移效率，从而提高纯化效率；(5)通过设置检测组件，可以对纯化过程中的各种参数进行检测，例如，通过检测分离容器内的液面位置是否达到设定的位置，进而将液面位置控制在合适的范围，可以有效提高纯化效率；(6)通过将分离容器设置为两端开口，使得磁珠可以从其一端开口进入，在纯化过程中混合有杂质以及混合有纯化目标物的液体可以从其另一端开口排出，提高了对分离容器的液体的收集效率；(7)利用本发明提供的纯化设备进行纯化时可以节省对粗样品过滤除杂所需要的昂贵耗材成本，以及节省常规磁珠纯化步骤中需要多次更换容器的耗材成本；(8)本发明提供的纯化设备所能纯化的粗样本体积不会受到制约，可以对1升以上级别的样本量进行纯化；(9)利用本发明提供的纯化设备提纯培养液(即粗样本)中的蛋白时，可以省去对培养液离心和过滤的步骤，避免了离心和过滤时对培养液中的纯化目标物造成损失，有效提高了纯化目标物的回收率。
上文已对基本概念做了描述，显然，对于本领域技术人员来说，上述发明披露仅仅作为示例，而并不构成对本说明书的限定。虽然此处并没有明确说明，本领域技术人员可能会对本说明书进行各种修改、改进和修正。该类修改、改进和修正在本说明书中被建议，所以该类修改、改进、修正仍属于本说明书示范实施例的精神和范围。
同时，本说明书使用了特定词语来描述本说明书的实施例。如“一个实施例”、“一实施例”和/或“一些实施例”意指与本说明书至少一个实施例相关的某一特征、结构或特点。因此，应强调并注意的是，本说明书中在不同位置两次或多次提及的“一实施例”或“一个实施例”或“一替代性实施例”并不一定是指同一实施例。此外，本说明书的一个或多个实施例中的某些特征、结构或特点可以进行适当的组合。
此外，本领域技术人员可以理解，本说明书的各方面可以通过若干具有可专利性的种类或情况进行说明和描述，包括任何新的和有用的工序、机器、产品或物质的组合或对他们的任何新的和有用的改进。相应地，本说明书的各个方面可以完全由硬件执行、可以完全由软件(包括固件、常驻软件、微码等)执行、也可以由硬件和软件组合执行。以上硬件或软件均可被称为“数据块”、“模块”、“引擎”、“单元”、“组件”或“系统”。此外，本说明书的各方面可能表现为位于一个或多个计算机可读介质中的计算机产品，该产品包括计算机可读程序编码。
此外，除非权利要求中明确说明，本说明书所述处理元素和序列的顺序、数字字母的使用或其他名称的使用，并非用于限定本说明书流程和方法的顺序。尽管上述披露中通过各种示例讨论了一些目前认为有用的发明实施例，但应当理解的是，该类细节仅起到说明的目的，附加的权利要求并不仅限于披露的实施例，相反，权利要求旨在覆盖所有符合本说明书实施例实质和范围的修正和等价组合。例如，虽然以上所描述的系统组件可以通过硬件设备实现，但是也可以只通过软件的解决方案得以实现，如在现有的服务器或移动设备上安装所描述的系统。
同理，应当注意的是，为了简化本说明书披露的表述，从而帮助对一个或多个发明实施例的理解，前文对本说明书实施例的描述中，有时会将多种特征归并至一个实施例、附图或对其的描述中。但是，这种披露方法并不意味着本说明书对象所需要的特征比权利要求中提及的特征多。实际上，实施例的特征要少于上述披露的单个实施例的全部特征。
一些实施例中使用了描述成分、属性数量的数字，应当理解的是，此类用于实施例描述的数字，在一些示例中使用了修饰词“大约”、“近似”或“大体上”等来修饰。除非另外说明，“大约”、“近似”或“大体上”表明所述数字允许有±20％的变化。相应地，在一些实施例中，说明书和权利要求中使用的数值数据均为近似值，该近似值根据个别实施例所需特点可以发生改变。在一些实施例中，数值数据应考虑规定的有效数位并采用一般位数保留的方法。尽管本说明书一些实施例中用于确认其范围广度的数值域和数据为近似值，在具体实施例中，此类数值的设定在可行范围内尽可能精确。
最后，应当理解的是，本说明书中所述实施例仅用以说明本说明书实施例的原则。其他的变形也可能属于本说明书的范围。因此，作为示例而非限制，本说明书实施例的替代配置可视为与本说明书的教导一致。相应地，本说明书的实施例不仅限于本说明书明确介绍和描述的实施例。

Claims (52)

  1. 一种纯化设备，所述纯化设备包括：
    混合组件，所述混合组件包括震荡平台；
    转移组件，所述转移组件包括磁性件；
    分离组件，所述分离组件包括分离容器和设置于所述分离容器中的过滤件，所述过滤件开设有过滤孔，所述过滤孔的孔径小于磁珠的直径。
  2. 根据权利要求1所述的纯化设备，所述磁性件包括磁棒，所述磁棒可沿所述震荡平台的高度方向运动。
  3. 根据权利要求2所述的纯化设备，所述转移组件还包括磁棒外套，所述磁棒外套套设在所述磁棒上，且所述磁棒外套能够相对所述磁棒沿套设方向运动。
  4. 根据权利要求2所述的纯化设备，所述转移组件还包括沿所述震荡平台的高度方向设置的第一轨道，所述磁棒可沿所述第一轨道滑动。
  5. 根据权利要求3所述的纯化设备，所述转移组件还包括沿所述震荡平台的高度方向设置的第一轨道和沿所述套设方向设置的第二轨道，所述第一轨道与所述磁棒外套相对固定，所述磁棒可沿所述第一轨道滑动，所述磁棒外套可沿所述第二轨道滑动。
  6. 根据权利要求1所述的纯化设备，所述纯化设备还包括防滴挡片，所述防滴挡片可伸缩地设置于所述第二轨道的底部。
  7. 根据权利要求2所述的纯化设备，所述转移组件还包括沿所述震荡平台的上方至所述分离容器的上方方向设置的第三轨道，所述磁棒可沿所述第三轨道滑动。
  8. 根据权利要求1所述的纯化设备，所述纯化设备还包括磁珠脱离组件，所述磁珠脱离组件用于将富集在所述磁性件上的所述磁珠从所述磁性件上脱离。
  9. 根据权利要求8所述的纯化设备，所述磁珠脱离组件包括冲洗组件；所述冲洗组件包括冲洗喷头，所述冲洗喷头设置在所述分离容器的上方。
  10. 根据权利要求9所述的纯化设备，所述冲洗组件还包括能够绕所述分离容器的中心轴线转动的旋转台，所述冲洗喷头设置于所述旋转台上。
  11. 根据权利要求10所述的纯化设备，所述分离容器的上方还设置有漏斗；所述漏斗的窄口端与所述分离容器的上方开口连通；所述旋转台能够绕所述漏斗的广口端转动。
  12. 根据权利要求11所述的纯化设备，所述分离容器包括重力柱，所述重力柱的上方开口与所述漏斗连通；所述分离组件还包括密封塞，所述重力柱的下方开口通过所述密封塞密封；与所述重力柱连通的排液管路的入口设置有穿刺针管，所述穿刺针管能够穿过所述密封塞。
  13. 根据权利要求12所述的纯化设备，所述分离组件还包括：
    支撑架，所述重力柱可以放置于所述支撑架内；
    适配结构，所述适配结构用于将不同的所述重力柱与所述支撑架进行配接。
  14. 根据权利要求13所述的纯化设备，所述适配结构包括：
    卡扣，设于所述支撑架的上方开口，用于限制所述重力柱晃动；
    压头，设于所述支撑架的下方开口，所述压头设有液体通道，所述压头的上方包括第一抵接面，所述液体通道的上方开口设于所述第一抵接面，所述液体通道的下方开口通过所述密封塞密封；
    所述重力柱的下方开口端包括与所述第一抵接面适配的第二抵接面，所述第一抵接面与所述第二抵接面抵接时，所述重力柱的下方开口伸入所述液体通道中。
  15. 根据权利要求14所述的纯化设备，所述漏斗的窄口端设置有压紧法兰，所述压紧法兰与所述卡扣配接时，将所述重力柱抵接于所述压头上。
  16. 根据权利要求14所述的纯化设备，所述重力柱包括不同规格的第一重力柱或第二重力柱，所述卡扣包括第一卡扣或第二卡扣，所述压头包括第一压头或第二压头；所述第一卡扣、所述第一压头与所述第一重力柱适配，所述第二卡扣、所述第二压头与所述第二重力柱适配。
  17. 根据权利要求12所述的纯化设备，所述旋转台可沿所述重力柱的高度方向运动。
  18. 根据权利要求17所述的纯化设备，所述分离组件还包括沿所述重力柱的高度方向设置的第一升降轨道，所述旋转台可沿所述第一升降轨道滑动。
  19. 根据权利要求13所述的纯化设备，所述支撑架可沿所述重力柱的高度方向移动。
  20. 根据权利要求19所述的纯化设备，所述分离组件还包括沿所述重力柱的高度方向设置的第二升降轨道，所述支撑架可沿所述第二升降轨道滑动。
  21. 根据权利要求13所述的纯化设备，所述支撑架可沿垂直于所述重力柱的高度方向移动。
  22. 根据权利要求21所述的纯化设备，所述分离组件还包括沿垂直于所述重力柱的高度方向设置的平移轨道，所述支撑架可沿所述平移轨道滑动。
  23. 根据权利要求1所述的纯化设备，所述纯化设备还包括第一检测组件；所述第一检测组件包括液位传感器，所述液位传感器用于检测所述分离容器中的液面位置是否达到设定的位置。
  24. 根据权利要求23所述的纯化设备，所述纯化设备还包括第一检测组件；所述第一检测组件包括液位传感器，所述液位传感器用于检测所述分离容器中的液面位置是否达到设定的位置。
  25. 根据权利要求24所述的纯化设备，所述第一检测组件还包括第二液位传感器，所述第二液位传感器设置在所述分离容器的第二位置，所述第一位置的高度高于所述第二位置的高度。
  26. 根据权利要求1所述的纯化设备，所述纯化设备还包括第二检测组件，所述第二检测组件用于检测加入到所述分离容器中的第一液体的体积。
  27. 根据权利要求1所述的纯化设备，所述纯化设备还包括第三检测组件，所述第三检测组件用于检测与所述分离容器连通的排液管路中第一液体中的杂质含量和/或第二液体中的纯化目标物含量。
  28. 根据权利要求27所述的纯化设备，所述第三检测组件包括与所述排液管路连通的含量检测传感器。
  29. 根据权利要求28所述的纯化设备，所述含量检测传感器包括紫外吸收检测器。
  30. 根据权利要求1所述的纯化设备，所述纯化设备还包括收集组件，所述收集组件用于收集来自所述分离容器的液体。
  31. 根据权利要求30所述的纯化设备，所述收集组件包括与所述分离容器的下方开口连通的加液口以及收集容器。
  32. 根据权利要求31所述的纯化设备，所述收集组件还包括废液暂存容器。
  33. 根据权利要求32所述的纯化设备，所述纯化设备还包括第四检测组件，用于检测所述废液暂存容器和/或收集容器中的液面位置是否达到设定的位置。
  34. 根据权利要求16所述的纯化设备，所述纯化设备包括第五检测组件，所述第五检测组件包括第一光电传感器，所述第一光电传感器用于检测所述支撑架内是否安装有所述第一重力柱或者所述第二重力柱。
  35. 根据权利要求31所述的纯化设备，所述收集组件还包括底座和收集容器支架，所述收集容器支架用于放置所述收集容器，所述收集容器支架安装于所述底座。
  36. 根据权利要求35所述的纯化设备，所述收集容器包括不同规格的第一收集容器或第二收集容器，所述收集容器支架包括第一收集容器支架或第二收集容器支架，所述第一收集容器支架用于放置所述第一收集容器，所述第二收集容器支架用于放置所述第二收集容器，所述第一收集容器支架的尺寸与所述第二收集容器支架的尺寸相同。
  37. 根据权利要求36所述的纯化设备，所述纯化设备还包括第六检测组件，所述第六检测组件包括第二光电传感器，所述第二光电传感器用于检测所述底座上是否安装有所述第一收集容器支架或者所述第二收集容器支架。
  38. 根据权利要求32所述的纯化设备，所述收集组件还包括沿所述废液暂存容器上方至所述收集容器上方方向设置的第四轨道，所述加液口可沿所述第四轨道滑动。
  39. 根据权利要求1所述的纯化设备，所述纯化设备还包括控制组件；所述控制组件用于控制所述混合组件进行混匀操作、控制所述转移组件中的所述磁性件运动至所述分离容器的上方开口。
  40. 根据权利要求39所述的纯化设备，所述混合组件还包括混匀容器，所述混匀容器设置于所述震荡平台上；所述控制组件包括驱动单元和控制单元，所述驱动单元与所述震荡平台驱动连接；
    所述控制单元用于生成第一指令，所述驱动单元用于根据所述第一指令驱动所述震荡平台震荡所述混匀容器。
  41. 根据权利要求40所述的纯化设备，所述纯化设备还包括磁珠脱离组件；所述驱动单元还与所述磁珠脱离组件以及所述磁性件驱动连接；
    所述控制单元还用于生成第二指令，所述驱动单元还用于根据所述第二指令驱动所述磁性件将所述磁珠转移至所述分离容器的上方开口以及驱动所述磁珠脱离组件将所述磁珠从所述磁性件上脱离。
  42. 根据权利要求41所述的纯化设备，所述磁珠脱离组件包括冲洗组件；所述驱动单元还与所述冲洗组件驱动连接；
    所述控制单元还用于生成第三指令，所述驱动单元还用于根据所述第三指令驱动所述冲洗组件向所述分离容器加入第一液体。
  43. 根据权利要求42所述的纯化设备，所述控制单元还用于生成第四指令，所述驱动单元还用于根据所述第四指令驱动所述冲洗组件向所述分离容器加入第二液体。
  44. 根据权利要求40的纯化设备，所述纯化设备还包括含量检测传感器和收集组件，所述驱动单元还与所述收集组件驱动连接；
    所述含量检测传感器用于根据与所述分离容器连通的排液管路中第一液体中的杂质含量生成第一含量检测信号和/或第二液体中的纯化目标物含量生成第二含量检测信号；
    所述控制单元还用于根据所述第二含量检测信号控制所述驱动单元驱动所述收集组件收集来自所述分离容器内的所述第二液体。
  45. 一种分离组件，所述分离组件包括分离容器和设置于所述分离容器中的过滤件，所述过滤件开设有过滤孔，所述过滤孔的孔径小于磁珠的直径。
  46. 根据权利要求45所述的分离组件，所述分离容器的两端均设置有开口。
  47. 根据权利要求45所述的分离组件，所述过滤件包括过滤板，所述过滤板的厚度方向与所述分离容器的高度方向平行，所述过滤板沿其厚度方向的横截面形状轮廓与所述分离容器的内部腔体沿其高度方向的横截面轮廓匹配。
  48. 一种基于纯化设备的控制方法，所述纯化设备包括：混合组件，所述混合组件包括震荡平台；转移组件，所述转移组件包括磁性件；分离组件，所述分离组件包括分离容器和设置于所述分离容器中的过滤件，所述过滤件开设有过滤孔，所述过滤孔的孔径小于磁珠的直径；磁珠脱离组件，所述磁珠脱离组件包括冲洗组件；收集组件，用于收集来自所述分离容器的液体；所述控制方法包括：
    控制所述混合组件进行混匀操作；
    控制所述转移组件中的所述磁性件将所述磁珠转移至所述分离容器的上方开口；
    控制所述磁珠脱离组件将所述磁珠从所述磁性件上脱离，使所述磁珠进入所述分离容器并堆积于所述过滤件上；
    控制所述冲洗组件向所述分离容器内加入第一液体；
    控制所述冲洗组件向所述分离容器内加入第二液体；
    控制所述收集组件收集来自所述分离容器的所述第一液体和/或所述第二液体。
  49. 根据权利要求48所述的控制方法，所述磁性件包括磁棒，所述转移组件包括沿所述震荡平台的高度方向设置的第一轨道以及沿所述震荡平台的上方至所述分离容器的上方方向设置的第三轨道，所述磁棒可沿所述第一轨道和所述第三轨道滑动；所述控制所述转移组件中的所述磁性件将所述磁珠转移至所述分离容器的上方开口包括：
    控制所述磁棒沿所述第一轨道和所述第三轨道滑动，将所述磁珠转移至所述分离容器的上方开口。
  50. 根据权利要求48所述的控制方法，所述控制所述磁珠脱离组件将所述磁珠从所述磁性件上脱离包括：
    控制所述冲洗组件冲洗所述磁珠。
  51. 根据权利要求48所述的控制方法，所述纯化设备还包括第三检测组件，所述第三检测组件用于检测与所述分离容器连通的排液管路中所述第一液体中的杂质含量；在控制所述冲洗组件向所述分离容器内加入第二液体之前，还包括：
    控制所述第三检测组件检测与所述分离容器连通的所述排液管路中所述第一液体中的杂质含量；
    当所述第一液体中的杂质含量达到第一含量阈值且维持检测时间阈值不变时，控制所述冲洗组件停止加入所述第一液体。
  52. 根据权利要求51所述的控制方法，所述第三检测组件还用于检测与所述分离容器连通的所述排液管路中所述第二液体中的纯化目标物含量；所述控制所述收集组件收集来自所述分离容器的所述第一液体和/或第二液体包括：
    控制所述第三检测组件检测与所述分离容器连通的所述排液管路中所述第二液体中的所述纯化目标物含量。
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