WO2023121114A1 - Dispositif de formation de micropuits et procédé pour la fabrication de disque ayant un micropuits - Google Patents

Dispositif de formation de micropuits et procédé pour la fabrication de disque ayant un micropuits Download PDF

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Publication number
WO2023121114A1
WO2023121114A1 PCT/KR2022/020255 KR2022020255W WO2023121114A1 WO 2023121114 A1 WO2023121114 A1 WO 2023121114A1 KR 2022020255 W KR2022020255 W KR 2022020255W WO 2023121114 A1 WO2023121114 A1 WO 2023121114A1
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WO
WIPO (PCT)
Prior art keywords
disk
assembly
well forming
support
well
Prior art date
Application number
PCT/KR2022/020255
Other languages
English (en)
Korean (ko)
Inventor
이문근
이태재
노동기
배남호
이경균
박유민
이석재
Original Assignee
한국과학기술원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020220173119A external-priority patent/KR20230096857A/ko
Application filed by 한국과학기술원 filed Critical 한국과학기술원
Publication of WO2023121114A1 publication Critical patent/WO2023121114A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C43/00—Compression moulding, i.e. applying external pressure to flow the moulding material; Apparatus therefor
    • B29C43/02—Compression moulding, i.e. applying external pressure to flow the moulding material; Apparatus therefor of articles of definite length, i.e. discrete articles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C43/00—Compression moulding, i.e. applying external pressure to flow the moulding material; Apparatus therefor
    • B29C43/32—Component parts, details or accessories; Auxiliary operations
    • B29C43/36—Moulds for making articles of definite length, i.e. discrete articles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C59/00—Surface shaping of articles, e.g. embossing; Apparatus therefor
    • B29C59/02—Surface shaping of articles, e.g. embossing; Apparatus therefor by mechanical means, e.g. pressing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C59/00—Surface shaping of articles, e.g. embossing; Apparatus therefor
    • B29C59/02—Surface shaping of articles, e.g. embossing; Apparatus therefor by mechanical means, e.g. pressing
    • B29C59/04—Surface shaping of articles, e.g. embossing; Apparatus therefor by mechanical means, e.g. pressing using rollers or endless belts

Definitions

  • the present invention relates to a microwell forming apparatus and a method for manufacturing a disk having microwells.
  • PCR Polymerase Chain Reaction
  • a digital PCR device capable of mounting a sample and processing and analyzing a PCR process is required.
  • the rotating type digital PCR apparatus rotates a disk to accommodate the sample, inserts the sample into the disk, spreads the sample on the inner wall of the disk by centrifugal force, and controls the temperature of the sample to perform the PCR process.
  • a plurality of micro-wells for accommodating samples are provided on the inner wall of a disk applied to a rotating digital PCR device.
  • microwells are spaced apart at equal intervals along the inner circumferential surface of the disk, and are formed in the form of grooves having a predetermined depth so that samples can be individually accommodated by centrifugal force when the disk rotates.
  • a disk and a film having a plurality of microwells are separately manufactured, and the film having the microwells is attached to the inner circumferential surface of the disk using an epoxy material adhesive to manufacture the disk.
  • the epoxy material used during bonding is autofluorescent during UV exposure, causing confusion in the process of detecting the fluorescence signal while rotating the amplified sample. There was a problem affecting the PCR test because the shape was not clearly molded.
  • the present invention has been made to solve the above problems, and an object of the present invention is to directly mold a micro-well on a disk, to prevent the self-fluorescence problem due to the shape defect of the micro-well and the use of a bonding agent, and to prevent the manufacturing process It is to provide a micro-well forming device capable of simplifying the process and a method for manufacturing a disk having micro-wells.
  • Another object of the present invention is to provide a microwell forming apparatus and a method for manufacturing a disc having a microwell, which can reduce manufacturing cost by preventing contamination of the disc and improving productivity and workability through simplification of the manufacturing process. .
  • a microwell forming apparatus for solving the above problems includes a well forming assembly configured to form microwells in a disk, the well forming assembly is disposed inside the disk, and the disk and a stamping roll configured to form the micro-wells on the inner circumferential surface of the disk by pressing the disk while being rotated in contact with the inner circumferential surface of the disk.
  • the stamping roll may have an outer diameter smaller than an inner diameter of the disc, and may press an inner circumferential surface of the disc at an eccentric position with respect to the disc.
  • the stamping roll may include a protrusion-shaped pattern formed on an outer circumferential surface, and the pattern may have a shape and size corresponding to the micro-well.
  • the well forming assembly includes a drive shaft configured to rotate the stamping roll; a roll support housing rotatably supporting the driving shaft; and a roll driving actuator configured to rotate the driving shaft.
  • the well forming assembly may include a heater accommodated inside the drive shaft and configured to heat the stamping roll by dissipating heat; and a temperature sensor configured to sense the temperature of the heater.
  • the well forming assembly may further include a cooling unit configured to prevent the well forming assembly from being heated by heat emitted from the heater accommodated inside the driving shaft.
  • the device may further include a disk holder spaced apart from the well forming assembly and configured to support an outer surface of the disk.
  • the disk holder may include a main shaft disposed opposite to the stamping roll contacting the inner circumferential surface of the disk and configured to support the disk while being rotated in a direction opposite to the direction in which the stamping roll rotates in contact with the outer circumferential surface of the disk; a plurality of driven shafts spaced apart from the main shaft and configured to support an outer circumferential surface of the disk at a plurality of locations; and a support plate disposed opposite to the stamping roll along an axial direction of the disk to support an outer surface of the disk.
  • the disk holder includes a support housing for supporting the main shaft, the plurality of driven shafts, and the support plate; And it may further include a support actuator configured to rotate the main shaft.
  • the disk holder may further include a temperature controller configured to heat or cool the main shaft.
  • the device may further include a roll transfer device configured to support the disk holder and move the well forming assembly in vertical and longitudinal directions.
  • the roll transfer device may include a support structure supporting the disk holder; a vertical drive assembly disposed on the support structure and configured to convert rotational motion into linear motion to move the well forming assembly in the vertical direction; and a front and rear driving assembly configured to support the well forming assembly, move up and down by the vertical driving assembly, and move the well forming assembly in the forward and backward directions.
  • the vertical drive assembly may include a plurality of support units spaced apart from each other in the vertical direction; a screw shaft rotatably coupled to the plurality of support units; a transfer nut that supports the front and rear driving assembly and is screwed to the screw shaft to move along an outer surface of the screw shaft when the screw shaft rotates and elevates the front and rear driving assembly; and a shaft driving actuator configured to rotate the screw shaft.
  • the vertical drive assembly may include an upper and lower guide rail fixed to the support structure; and a vertical transfer block coupled to the front and rear driving assembly and configured to slide along the vertical guide rail while moving up and down by the front and rear driving assembly.
  • the front and rear driving assembly may include a lifting bracket coupled to and supported by the transfer nut and the vertical transfer block, and moving the well forming assembly in the vertical direction while being moved up and down by the transfer nut; a forward and backward driving actuator disposed on the elevating bracket and configured to move the well forming assembly in the forward and backward directions while extending or contracting; Front and rear guide blocks coupled to the elevating bracket and disposed in a fixed state; and a forward/backward moving rail coupled to the well forming assembly and sliding in the forward/backward direction by being guided by the forward/backward guide block when the well forming assembly moves.
  • a shaft holder configured to rotatably support the driving shaft may be further included.
  • the shaft holder may include a post member; a holding plate coupled to and supported by the post member; and a support roller rotatably coupled to the holding plate and rotated by the driving shaft while supporting the driving shaft.
  • the well forming assembly may further include a vacuum chamber accommodated therein and converting an internal space into a vacuum or atmospheric pressure state.
  • the vacuum chamber includes a base plate; a chamber housing coupled to the base plate, disposed above the base plate, accommodating the well forming assembly therein, and having an entrance at one side thereof; a vacuum line communicating with the chamber housing and configured to form the chamber housing in a vacuum state; And it may include an opening and closing door configured to open and close the entrance.
  • the vacuum chamber may further include a detection sensor configured to detect an object entering or exiting the chamber housing from a front of the entrance.
  • a frame assembly configured to support the vacuum chamber and dispose it at a position spaced apart from the ground may be further included.
  • a controller configured to control driving of the well forming assembly, the disk holder, and the roll transfer device may be further included.
  • a method for manufacturing a disk with micro-wells according to an embodiment of the present invention for solving the above problems includes injection molding a disk and a cover; forming microwells in the disk; and forming a disk assembly by coupling the cover to the disk.
  • Forming the microwells on the disk may include fixing the disk; and forming the microwells on the inner circumferential surface of the disc by pressing and heating the inner circumferential surface of the disc at a position eccentric with respect to the center of the disc.
  • micro-wells are directly molded on the disk, problems of autofluorescence due to shape defects of the micro-wells and the use of a bonding agent can be prevented, and the manufacturing process can be simplified.
  • microwells are directly formed on a disk to simplify the manufacturing process, contamination of the disk due to bonding is prevented, productivity and workability are improved, and manufacturing cost can be reduced.
  • Effects according to the present invention are not limited by the contents exemplified above, and more various effects are included in the present invention.
  • FIG. 1 is a conceptual diagram schematically illustrating a state in which a disk is pressed by a well forming assembly of a micro well forming apparatus according to an embodiment of the present invention.
  • FIG. 2 is a side view illustrating a well forming assembly of a micro well forming apparatus according to an embodiment of the present invention.
  • FIG. 3 is an exploded perspective view of a well forming assembly of a micro well forming apparatus according to an embodiment of the present invention.
  • FIG. 4 is a side view illustrating a state in which a heater and a temperature sensor are disposed inside a well forming assembly of a micro well forming apparatus according to an embodiment of the present invention.
  • FIG. 5 is a conceptual diagram schematically illustrating a state in which a disk holder of a microwell forming apparatus according to an embodiment of the present invention supports a disk.
  • FIG. 6 is a perspective view illustrating a disk holder of a microwell forming apparatus according to an embodiment of the present invention.
  • FIG. 7 is a perspective view illustrating a state in which a disk holder and a well forming assembly are coupled to a roll transfer device of a micro well forming apparatus according to an embodiment of the present invention.
  • FIG. 8 is a perspective view illustrating a vertical driving assembly of a roll transfer device of a microwell forming apparatus according to an embodiment of the present invention.
  • FIG. 9 is a perspective view showing a front and rear drive assembly of a roll transfer device of a microwell forming apparatus according to an embodiment of the present invention.
  • FIG. 10 is a side view illustrating a state in which the front and rear driving assemblies and well forming assemblies of the roll transfer device of the microwell forming apparatus according to an embodiment of the present invention are coupled.
  • FIG. 11 is a perspective view illustrating a shaft holder of a microwell forming apparatus according to an embodiment of the present invention.
  • FIG. 12 is a perspective view illustrating a vacuum chamber of a microwell forming apparatus according to an embodiment of the present invention.
  • FIG. 13 is a perspective view showing a vacuum chamber of the microwell forming apparatus according to an embodiment of the present invention viewed from the bottom.
  • FIG. 14 is a perspective view illustrating a state in which a frame assembly of a microwell forming apparatus according to an embodiment of the present invention is coupled to a vacuum chamber.
  • 15 is a conceptual diagram schematically illustrating a disk manufacturing system according to an embodiment of the present invention.
  • 16 is a flowchart illustrating a method of manufacturing a disk having microwells according to an embodiment of the present invention.
  • 17 is a flowchart illustrating a process of forming microwells in a disc in a method of manufacturing a disc having microwells according to an embodiment of the present invention.
  • a “module” or “unit” for a component used in this specification performs at least one function or operation.
  • a “module” or “unit” may perform a function or operation by hardware, software, or a combination of hardware and software.
  • a plurality of “modules” or “units” other than “modules” or “units” to be executed in specific hardware or to be executed in at least one processor may be integrated into at least one module. Singular expressions include plural expressions unless the context clearly dictates otherwise.
  • FIG. 1 is a conceptual diagram schematically illustrating a state in which a disk is pressed by a well forming assembly of a micro well forming apparatus according to an embodiment of the present invention.
  • a microwell forming apparatus 100 includes a well forming assembly 110.
  • the well forming assembly 110 is configured to form micro wells MW in a dish-shaped disk D having one surface opened.
  • the well forming assembly 110 includes a stamping roll 111 .
  • the stamping roll 111 forms micro wells MW on the inner circumferential surface of the disk D while being disposed inside the disk D.
  • the stamping roll 111 is accommodated inside the disk (D) and disposed at a set position, and presses the disk (D) while rotating in contact with the inner circumferential surface of the disk (D). It is configured to form a micro well (MW) in.
  • the stamping roll 111 may have an outer diameter (OD) smaller than an inner diameter (ID) of the disk (D).
  • the central axis RC of the stamping roll 111 contacting the inner circumferential surface of the disk D may be disposed at a different position from the central axis DC of the disk D.
  • the stamping roll 111 accommodated in the disk (D) may press the inner circumferential surface of the disk (D) at an eccentric position with respect to the disk (D).
  • the disk D pressed by the stamping roll 111 may be rotated in the circumferential direction by the stamping roll 111 .
  • the stamping roll 111 may include a pattern 111A forming micro wells MW on an inner circumferential surface of the disk D.
  • the pattern 111A may be formed in plurality along the outer circumferential surface of the stamping roll 111 and formed in a protrusion shape.
  • the protrusion-shaped pattern 111A may have a shape and size corresponding to the micro-well MW.
  • the protruding pattern 111A may be formed in a column shape having a height of 10 to 100 ⁇ m and an inclination angle of 1 to 20 degrees in a protruding direction.
  • the protrusion-shaped pattern 111A may be formed in a columnar shape having width, length, and height of 200 x 200 x 50 ⁇ m, respectively, and an inclination angle of 10 degrees in the protrusion direction.
  • the protrusion-shaped pattern 111A is not necessarily limited thereto, and may be changed in various shapes and sizes.
  • FIG. 2 is a side view showing a well forming assembly of a microwell forming apparatus according to an embodiment of the present invention
  • FIG. 3 is an exploded perspective view of the well forming assembly of a microwell forming apparatus according to an embodiment of the present invention.
  • the well forming assembly 110 includes a drive shaft 112 configured to rotate the stamping roll 111, a roll support housing 113 rotatably supporting the drive shaft 112, and , a roll drive actuator 114 configured to rotate the drive shaft 112 may be further included.
  • the roll drive actuator 114 includes a motor generating rotational force, a drive pulley (not shown) rotated by the motor, a power transmission pulley coupled to the drive shaft 112 and rotated together with the drive shaft 112, and a drive pulley. It may include a timing belt (not shown) and a tensioner (not shown) for controlling the tension of the timing belt that connects the power transmission pulley to transmit the rotational force of the driving pulley to the power transmission pulley.
  • the roll driving actuator 114 is not necessarily limited thereto, and may be implemented in various structures capable of rotating the driving shaft 112 .
  • FIG. 4 is a side view illustrating a state in which a heater and a temperature sensor are disposed inside a well forming assembly of a micro well forming apparatus according to an embodiment of the present invention.
  • the well forming assembly 110 may further include a heater 115 and a temperature sensor 116 .
  • the heater 115 may be accommodated inside the driving shaft 112 and heat the stamping roll 111 by dissipating heat.
  • the heater 115 may generate heat at a preset temperature when the drive shaft 112 rotates, and may be configured to automatically cut off power when the heater 115 generates heat at a temperature higher than a set critical temperature.
  • the temperature sensor 116 is accommodated inside the driving shaft 112 and can detect heating temperatures of the heater 115 and the stamping roll 111 at a location spaced apart from the heater 115 and transmit a signal thereof to an external device. there is.
  • the well forming assembly 110 may form the micro wells MW on the inner circumferential surface of the disk D by heating and pressurizing the disk D.
  • the well forming assembly 110 may further include a cooling unit 117 .
  • the cooling unit 117 may be configured to prevent the well forming assembly 110 from being heated by heat emitted from the heater 115 accommodated inside the driving shaft 112 .
  • the cooling unit 117 includes at least one cold air injection port communicating with the roll support housing 113 in which the heater 115 is accommodated, a cold air supply tube for supplying cold air of a set temperature to the cold air injection port, and a cold air supply.
  • a cold air supply source (not shown) for supplying cold air to the tube may be included.
  • the cooling unit 117 is not necessarily limited thereto, and may be implemented in various shapes and structures capable of preventing the well forming assembly 110 from being heated by heat emitted from the heater 115 .
  • FIG. 5 is a conceptual diagram schematically illustrating a state in which a disk holder of a microwell forming apparatus according to an embodiment of the present invention supports a disk
  • FIG. 6 is a perspective view showing a disk holder of a microwell forming apparatus according to an embodiment of the present invention. am.
  • the microwell forming apparatus 100 may further include a disk holder 120 .
  • the disk holder 120 may be configured to be spaced apart from the well forming assembly 110 and support the outer surface of the disk D at a plurality of locations.
  • the disk holder 120 may include a main shaft 121 , a plurality of driven shafts 122 and a support plate 123 .
  • the main shaft 121 is disposed opposite to the stamping roll 111 in contact with the inner circumferential surface of the disk D, and the main shaft 121 is in contact with the outer circumferential surface of the disk D and the direction in which the stamping roll 111 rotates It can support the disk (D) while being rotated in the opposite direction.
  • the plurality of driven shafts 122 are spaced apart from the main shaft 121 and may support the outer circumferential surface of the disk D at a plurality of locations.
  • the plurality of driven shafts 122 may support the outer circumferential surface of the disk D at two or more positions.
  • An actuator (not shown) for providing rotational driving force is connected to at least one of the main shaft 121 and the plurality of middle shafts 122 to rotate at least one of the main shaft 121 and the plurality of middle shafts 122 can drive
  • a holding plate capable of supporting the end of the disk D may be further provided at the ends of the main shaft 121 and the plurality of driven shafts 122 . Through this, it is possible to prevent the disk D supported by the main shaft 121 and the plurality of driven shafts 122 from being moved or separated in the axial direction.
  • the support plate 123 is disposed to face the stamping roll 111 along the axial direction of the disk D, and may support the outer surface of the disk D along the axial direction of the disk D.
  • the disk holder 120 is configured to rotate the main shaft 121, the plurality of driven shafts 122 and the support housing 124 for supporting the support plate 123, and the main shaft 121.
  • a support actuator 125 may be further included.
  • the disk holder 120 may further include a temperature controller 126 .
  • the temperature control device 126 may be accommodated inside the support actuator 125 and may be configured to heat or cool the main shaft 121 to a set temperature.
  • FIG. 7 is a perspective view illustrating a state in which a disk holder and a well forming assembly are coupled to a roll transfer device of a micro well forming apparatus according to an embodiment of the present invention.
  • the microwell forming apparatus 100 may further include a roll transfer apparatus 130 .
  • the roll transfer device 130 may be configured to support the disk holder 120 and move the well forming assembly 110 in a vertical direction and a longitudinal direction.
  • the roll transfer device 130 may include a support structure 131 , an up and down drive assembly 132 and a front and rear drive assembly 133 .
  • the support structure 131 is formed in a tower structure having a predetermined height, and may support the disk holder 120 and the vertical driving assembly 132 .
  • the support structure 131 includes a first plate disposed parallel to the ground, and a second plate vertically disposed on an upper surface of the first plate and supporting the disk holder 120 and the vertical driving assembly 132. It may include a plate and a plurality of ribs that are vertically disposed on the upper surface of the first plate and support both ends of the second plate.
  • the support structure 131 is not necessarily limited thereto and may be implemented in various structures and shapes.
  • the vertical driving assembly 132 may be disposed on the support structure 131 and convert rotational motion into linear motion to move the well forming assembly 110 in the vertical direction.
  • FIG. 8 is a perspective view illustrating a vertical driving assembly of a roll transfer device of a microwell forming apparatus according to an embodiment of the present invention.
  • the vertical driving assembly 132 includes a plurality of support units 132A spaced apart in the vertical direction and a screw shaft 132B rotatably coupled to the plurality of support units 132A.
  • the up and down drive assembly 132 may include a feed nut 132C and a shaft drive actuator 132D.
  • the transfer nut 132C supports the front and rear drive assembly 133 and is screwed to the outer surface of the screw shaft 132B so that it can move upward or downward along the outer surface of the screw shaft 132B when the screw shaft 132B rotates. there is. Accordingly, the transfer nut 132C may move the front and rear drive assembly 133 up and down.
  • the shaft drive actuator 132D may be connected to the screw shaft 132B and configured to rotate the screw shaft 132B by generating rotational force.
  • the shaft drive actuator 132D includes a motor (not shown) configured to generate rotational force, a first pulley (not shown) coupled to an end of the motor and rotated by the motor, and a screw shaft 132B.
  • a second pulley (not shown) coupled to an end and rotated together with the screw shaft 132B, and a drive belt connecting the first pulley and the second pulley to each other and transmitting the rotational force of the first pulley to the second pulley (not shown). ) may be included.
  • the shaft drive actuator 132D is not necessarily limited thereto and may be implemented in various structures and shapes.
  • the vertical driving assembly 132 may further include an upper and lower guide rail 132E and an upper and lower transfer block 132F.
  • the upper and lower guide rails 132E may be fixedly disposed on the front surface of the support structure 131 and may be disposed such that a longitudinal direction thereof faces an upper and lower direction.
  • the vertical transfer block 132F may be coupled to the front and rear drive assembly 133 and slide along the top and bottom guide rails 132E while being moved up and down by the front and rear drive assembly 133 .
  • the front and rear driving assembly 133 supports the well forming assembly 110, is moved up and down by the vertical driving assembly 132, and may be configured to move the well forming assembly 110 forward and backward. there is.
  • FIG. 9 is a perspective view showing a front and rear drive assembly of a roll transfer device of a microwell forming apparatus according to an embodiment of the present invention.
  • the front and rear driving assembly 133 may include a lifting bracket 133A, a front and rear driving actuator 133B, a front and rear guide block 133C and a front and rear moving rail 133D.
  • the elevating bracket 133A is coupled to and supported by the transport nut 132C and the vertical transport block 132F, and may move the well forming assembly 110 vertically while being moved up and down by the transport nut 132C.
  • the elevating bracket 133A includes a connecting plate coupled to the conveying nut 132C and the vertical conveying block 132F, and coupled to the lower end of the linking plate to include a forward and backward driving actuator 133B, a forward and backward guide block 133C, and It may include a support plate supporting the front and rear movement rail 133D, and a plurality of ribs connecting and supporting the support plate and the connecting plate.
  • the elevating bracket 133A is not necessarily limited thereto and may be implemented in various structures and shapes.
  • the forward and backward driving actuator 133B may be disposed on the elevating bracket 133A and move the well forming assembly 110 forward and backward while extending or contracting.
  • the front and rear driving actuator 133B may include a cylinder body configured to allow fluid to flow into or out of the cylinder body, and a pressure rod that is expanded or contracted by the fluid flowing into or out of the cylinder body.
  • the front and rear driving actuator 133B is not necessarily limited thereto and may be implemented in various structures and shapes.
  • FIG. 10 is a side view illustrating a state in which the front and rear driving assemblies and well forming assemblies of the roll transfer device of the microwell forming apparatus according to an embodiment of the present invention are coupled.
  • the front and rear guide block 133C may be disposed in a fixed state by being coupled to the lower surface of the elevating bracket 133A.
  • a plurality of front and rear guide blocks 133C may be disposed on the lower surface of the elevating bracket 133A along the front and rear directions.
  • the forward/backward movement rail 133D may be coupled to the well forming assembly 110 and disposed in a fixed state, and may be coupled to the forward/backward guide block 133C.
  • the forward and backward movement rail 133D moves together with the well forming assembly 110 and is guided by the front and rear guide blocks 133C in the forward and backward directions. It can slide.
  • the microwell forming apparatus 100 may further include a shaft holder 140 .
  • the shaft holder 140 may be coupled to the support structure 131 and configured to rotatably support the driving shaft 112 .
  • FIG. 11 is a perspective view illustrating a shaft holder of a microwell forming apparatus according to an embodiment of the present invention.
  • the shaft holder 140 includes a plurality of post members 141 coupled to and supported by the support structure 131, and a holding plate 142 coupled to and supported by the plurality of post members 141. ) may be included.
  • the shaft holder 140 may include a plurality of support rollers 143 rotatably coupled to the holding plate 142 and rotatably supporting the driving shaft 112 .
  • the plurality of support rollers 143 may be configured to be rotated by the driving shaft 112 while supporting the driving shaft 112 .
  • FIG. 12 is a perspective view illustrating a vacuum chamber of a microwell forming apparatus according to an embodiment of the present invention.
  • the microwell forming apparatus 100 may further include a vacuum chamber 150 .
  • the vacuum chamber 150 may accommodate the well forming assembly 110 , the disk holder 120 , the roll transfer device 130 and the shaft holder 140 therein. Further, the vacuum chamber 150 forms an internal space in a vacuum state when the micro wells MW are formed on the disk D, and creates an atmospheric pressure state when the micro wells MW are formed on the disk D. can be converted to
  • FIG. 13 is a perspective view showing a vacuum chamber of the microwell forming apparatus according to an embodiment of the present invention viewed from the bottom.
  • the vacuum chamber 150 may include a base plate 151 , a chamber housing 152 , a vacuum line 153 and an opening/closing door 154 .
  • the base plate 151 may support the roll transfer device 130 , the chamber housing 152 , the vacuum line 153 , and the opening/closing door 154 .
  • the roll transfer device 130 and the chamber housing 152 are supported on the upper surface of the base plate 151, and the vacuum line 153 and the opening/closing door 154 are supported on the lower surface of the base plate 151.
  • the chamber housing 152 may be coupled to the base plate 151 and disposed on the base plate 151 .
  • the chamber housing 152 may have a polyhedron structure in which a predetermined space is formed. Accordingly, the well forming assembly 110, the disk holder 120, the roll transfer device 130, and the shaft holder 140 may be accommodated in the chamber housing 152 coupled to the base plate 151.
  • An entrance 152A may be provided on one side of the chamber housing 152 to communicate the inner space and the outer space of the chamber housing 152 and allow the disk D to enter and exit.
  • the chamber housing 152 includes a front plate, a back plate disposed opposite to the front plate in the front-back direction, a plurality of side plates disposed at both ends of the front plate and the back plate, and the plates (front plate, back plate). and a top plate disposed on an upper end of the plurality of side plates).
  • transparent viewing windows may be provided on each of the front plate, the back plate, and the plurality of side plates.
  • an entrance 152A may be provided on the front plate, and at least one vacuum connector (not shown) may be provided on the back plate.
  • a switch for operating the vacuum line 153 may be provided on the top plate.
  • the chamber housing 152 is not necessarily limited thereto and may be implemented in various structures and shapes.
  • the vacuum line 153 is coupled to the lower surface of the base plate 151 to communicate with the chamber housing 152, and sucks in air inside the chamber housing 152 according to a set control command so that the chamber housing ( 152) can be formed in a vacuum state.
  • the vacuum line 153 passes through the base plate 151 and is connected to a pipe communicating with the inner space of the chamber housing 152, a valve that opens and closes a flow path of the pipe, and a pipe, and generates a suction force so that the chamber housing ( 152) may include a vacuum pump (not shown) that forms the inner space in a vacuum state.
  • the vacuum line 153 is not necessarily limited thereto, and may be implemented in various structures and shapes.
  • the opening and closing door 154 may be configured to open and close the entrance (152A).
  • the opening and closing door 154 includes a rail support bar coupled to the lower surface of the base plate 151, a guide rail that is partially coupled to the front plate of the chamber housing 152 and supported by being coupled to the other rail support bar,
  • An opening and closing plate that opens and closes the entrance (152A) while moving up and down along the guide rail, connects the rail support bar and the opening and closing plate, and closes the entrance (152A) by raising the opening and closing plate while extending, or lowers the opening and closing plate while contracting ( 152A) may include an opening/closing drive actuator that opens.
  • a through hole through which the guide rail and the opening/closing plate can pass may be formed in the base plate 151 .
  • the opening and closing door 154 is not necessarily limited thereto, and may be implemented in various structures and shapes.
  • the vacuum chamber 150 may further include a detection sensor 155 .
  • the detection sensor 155 may be configured to detect an object entering or exiting the chamber housing 152 from the front of the entrance 152A.
  • the detection sensor 155 may be implemented as an area sensor.
  • the detection sensor 155 is not necessarily limited thereto and may be implemented in various structures and shapes.
  • FIG. 14 is a perspective view illustrating a state in which a frame assembly of a microwell forming apparatus according to an embodiment of the present invention is coupled to a vacuum chamber.
  • the microwell forming apparatus 100 may further include a frame assembly 160 .
  • the frame assembly 160 may be configured to support the vacuum chamber 150 and place it at a position spaced apart from the ground.
  • the frame assembly 160 may include a profile assembly structure (not shown) that forms the framework of a hexahedral structure. A part of the opening and closing door 154 is accommodated inside the profile assembly structure, and the base plate 151 may be seated and supported on the upper surface of the profile assembly structure.
  • the frame assembly 160 may include a reinforcing profile (not shown) disposed inside the profile assembly structure to support the profile assembly structure.
  • the frame assembly 160 includes a front cover detachably coupled to the front of the profile assembly structure, a back cover (not shown) detachably coupled to the rear of the profile assembly structure, and a plurality of coupled to both sides of the profile assembly structure.
  • the frame assembly 160 may include a side cover and a bottom cover (not shown) coupled to the lower surface of the profile assembly structure.
  • stoppers may be provided on the front and rear surfaces of the profile assembly structure to support the front cover and the back cover.
  • catches capable of being gripped by an operator may be provided on the front cover and the back cover, respectively, and vents may be provided on the plurality of side covers.
  • the frame assembly 160 is disposed on the bottom surface of the profile assembly structure and rotates in contact with the ground caster (not shown) configured to move the profile assembly structure, disposed on the bottom surface of the profile assembly structure and extending in the vertical direction While being supported on the ground may further include a pedestal for separating the caster from the ground.
  • the frame assembly 160 is not necessarily limited thereto and may be implemented in various structures and shapes.
  • a circuit breaker, an electromagnetic contactor, a power supply, a noise filter, a converter, a relay, a buzzer, an outlet, a bus bar, a terminal block, and a duct may be disposed inside the frame assembly 160 .
  • the interior of the frame assembly 160 does not necessarily accommodate only the above-described components, and various components may be accommodated as needed.
  • the microwell forming apparatus 100 may further include a controller 170 .
  • the controller 170 is electrically connected to the well forming assembly 110, the disk holder 120, the roll transfer device 130, and the vacuum chamber 150, and receives a control signal input from the outside to form the well forming assembly 110. ), the disc holder 120, the roll transfer device 130, and the driving of the vacuum chamber 150 can be controlled.
  • the controller 170 may include a control box disposed on the base plate 151 .
  • the controller 170 is accommodated in the control box to control driving of the well forming assembly 110, the disk holder 120, the roll transfer device 130 and the vacuum chamber 150, and the well forming assembly 110, It may include a control unit (not shown) configured to detect driving states and abnormalities of the disk holder 120, the roll transfer device 130, and the vacuum chamber 150 in real time.
  • the controller 170 controls the operation and control conditions of the well forming assembly 110, the disk holder 120, the roll transfer device 130, and the vacuum chamber 150, as well as the micro well forming device 100. It may include a display configured to display all information related to its operation.
  • the display may include a first display unit (not shown) displaying almost all information related to control and operation, and a second display unit (not shown) configured to display numbers or characters.
  • the controller 170 may include a command input unit (not shown) capable of inputting a control signal to the control unit through manipulation by an operator.
  • the command input unit includes a touch panel integrally provided with the display, an emergency stop button coupled to the control box and exposed to the outside to stop the operation of the microwell forming apparatus 100 in an emergency, and power to the microwell forming apparatus 100. It may include a power supply button to supply power.
  • the controller 170 may include a speaker (not shown) generating preset sounds according to work conditions.
  • controller 170 is connected to the control unit through a wired/wireless communication method, transmits a control signal to the control unit, or controls a control server (not shown) and a control unit configured to receive control-related information from the control unit.
  • a communication module (not shown) connecting the server may be further included.
  • the controller 170 is not necessarily limited thereto and may be implemented to further include various functions.
  • each configuration for explaining the method for manufacturing a disk having microwells according to an embodiment of the present invention (hereinafter referred to as 'disc manufacturing method') is used while describing the microwell forming apparatus 100 for convenience of description.
  • 'disc manufacturing method' each configuration for explaining the method for manufacturing a disk having microwells according to an embodiment of the present invention
  • 15 is a conceptual diagram schematically illustrating a disk manufacturing system according to an embodiment of the present invention.
  • the disk manufacturing method is performed through the disk manufacturing system 1 .
  • the disk manufacturing system 1 includes an injection device 200 configured to injection mold a disk D and a cover TC, a cleaning device 300 configured to clean the disk D and cover TC, and a disk ( D) a micro well forming device 100 configured to mold a micro well MW and a coupling device 400 configured to couple a cover TC to a disk D to form a disk assembly DA. do.
  • the cleaning device 300 is included in the disk manufacturing system 1, but the entire process of manufacturing the disk D is performed in a clean room, or the disk manufacturing system 1 In the case of being connected to an independent clean space capable of creating a clean environment, the above-described cleaning device 300 may be selectively applied to the disk manufacturing system 1 .
  • the microwell forming apparatus 100 may include a well forming assembly 110, a disk holder 120, a roll transfer device 130, and a vacuum chamber 150. .
  • the well forming assembly 110 is accommodated inside the disk D, is disposed at an eccentric position with respect to the center of the disk D, and presses the disk D while being rotated in contact with the inner circumferential surface of the disk D. And heating may be configured to form a micro-well (MW) on the inner circumferential surface of the disk (D).
  • MW micro-well
  • the disk holder 120 may be spaced apart from the well forming assembly 110 and rotated together with the disk D while supporting the outer surface of the disk D.
  • the roll transfer device 130 may be configured to support the disk holder 120 and move the well forming assembly 110 in a vertical direction and a longitudinal direction.
  • the vacuum chamber 150 accommodates the well forming assembly 110, the disk holder 120, and the roll transfer device 130 therein, and selectively vacuums the inner space when forming the micro well MW on the disk D. It can be configured to remain in the state.
  • a method for manufacturing a disk will be described with reference to the disk manufacturing system 1 described above.
  • 16 is a flowchart illustrating a method of manufacturing a disk having microwells according to an embodiment of the present invention.
  • the injection device 200 injection molds the disk D and the cover TC (S110).
  • the cleaning device 300 when the disk D and the cover TC are injection-molded by the injection device 200, the cleaning device 300 inserts the disk D and the cover TC is washed to remove foreign substances from the disk D and the cover TC.
  • the cleaning process is for removing foreign substances such as dust or organic matter, and the entire process of manufacturing the disc D is performed in a clean room or an independent clean space where the disc manufacturing system 1 can create a clean environment.
  • the microwell molding device 100 forms the disk D A microwell MW is formed (S120). A process of forming the microwell MW in the disk D by the microwell forming apparatus 100 will be described in more detail.
  • 17 is a flowchart illustrating a process of forming microwells in a disc in a method of manufacturing a disc having microwells according to an embodiment of the present invention.
  • the disk D and the cover TC are injection-molded by the injection device 200, the disk D is mounted and fixed to the disk holder 120 (S121 ).
  • the disk D may be mounted on the disk holder 120 by a worker or mounted on the disk holder 120 by a separately manufactured disk supply device (not shown).
  • the disk supply device includes a tray (not shown) loaded with a plurality of disks D on which microwells MW are not formed, and gripping the disks D from the tray to supply them to the disk holder 120. It may include a configured disk transfer member (not shown) and the like. However, the disk supply device is not necessarily limited thereto and may have more diverse structures and functions.
  • the vacuum chamber 150 maintains the space where the disk D is accommodated at normal pressure, that is, at atmospheric pressure, or forms a vacuum.
  • the internal space in which the disk (D) is accommodated is maintained at atmospheric pressure, or the disk (D) is accommodated. It converts into a vacuum state by inhaling and discharging the air in the internal space.
  • the roll transfer device 130 transfers the well forming assembly 110 into the disk D and brings it into contact with the inner circumferential surface of the disk D.
  • the well forming assembly 110 When the well forming assembly 110 contacts the inner circumferential surface of the disk D, the well forming assembly 110 pressurizes and heats the inner circumferential surface of the disk D while being rotated at a position eccentric with respect to the center of the disk D, Micro wells MW are formed on the inner circumferential surface of the disk D (S122).
  • the well forming assembly 110 stops rotating, and the roll transfer device 130 separates the well forming assembly 110 from the disk D.
  • the vacuum chamber 150 opens an entrance.
  • the coupling device 400 couples the cover TC to the disk D to form a disk assembly DA ( S130).
  • the coupling device 400 may be configured to fuse the cover TC to the disk D using ultrasonic waves or heat.
  • the coupling device 400 is not necessarily limited thereto, and may be configured to bond the cover TC to the disk D using an adhesive or an adhesive film or laser bonding.
  • the cleaning device 300 may selectively clean the disk assembly DA to remove remaining foreign substances from the disk assembly DA.
  • the cleaning process when the entire process of manufacturing the disk D is performed in a clean room, or when the disk manufacturing system 1 is connected to an independent clean space capable of creating a clean environment, the cleaning process described above A cleaning process may optionally be applied.
  • the micro wells MW are directly molded on the disk D, the problem of autofluorescence due to the shape defect of the micro wells MW and the use of a bonding agent is prevented, and the manufacturing process is simplified. can do.
  • micro-well (MW) is directly molded on the disk (D) to simplify the manufacturing process, contamination of the disk (D) due to bonding is prevented, and productivity and workability are improved. Manufacturing cost can be reduced.

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif de formation de micropuits permettant de former directement un micropuits sur un disque pour éviter l'apparition des problèmes d'une forme médiocre d'un micropuits et d'une autofluorescence due à l'utilisation d'un agent de liaison et simplifier un processus de fabrication. Un dispositif de formation de micropuits selon un mode de réalisation de la présente invention comprend un ensemble de formation de puits configuré pour former un micropuits sur un disque, et l'ensemble de formation de puits comporte un rouleau d'estampage disposé dans le disque et configuré pour presser le disque alors qu'il tourne dans un état dans lequel il a été mis en contact avec la surface périphérique interne du disque, permettant ainsi de former un micropuits sur la surface périphérique interne du disque.
PCT/KR2022/020255 2021-12-23 2022-12-13 Dispositif de formation de micropuits et procédé pour la fabrication de disque ayant un micropuits WO2023121114A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20210186461 2021-12-23
KR10-2021-0186461 2021-12-23
KR10-2022-0173119 2022-12-12
KR1020220173119A KR20230096857A (ko) 2021-12-23 2022-12-12 마이크로 웰 성형 장치 및 마이크로 웰을 가지는 디스크 제조 방법

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WO2023121114A1 true WO2023121114A1 (fr) 2023-06-29

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH068241A (ja) * 1992-04-25 1994-01-18 Paul Troester Mas Fab ゴム破片の破砕方法とその装置
JP2002292600A (ja) * 2001-03-30 2002-10-08 Mitsui Chemicals Inc マイクロ配管およびその製造方法
CN102967715A (zh) * 2012-10-25 2013-03-13 杭州普施康生物科技有限公司 一种新型酶联免疫生化光盘检测系统
JP2016101629A (ja) * 2014-11-28 2016-06-02 デクセリアルズ株式会社 マイクロ流路作製用原盤、転写物、およびマイクロ流路作製用原盤の製造方法
JP2016124263A (ja) * 2015-01-08 2016-07-11 株式会社クラレ 円筒微細構造体の製造方法及びレプリカロールの製造方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH068241A (ja) * 1992-04-25 1994-01-18 Paul Troester Mas Fab ゴム破片の破砕方法とその装置
JP2002292600A (ja) * 2001-03-30 2002-10-08 Mitsui Chemicals Inc マイクロ配管およびその製造方法
CN102967715A (zh) * 2012-10-25 2013-03-13 杭州普施康生物科技有限公司 一种新型酶联免疫生化光盘检测系统
JP2016101629A (ja) * 2014-11-28 2016-06-02 デクセリアルズ株式会社 マイクロ流路作製用原盤、転写物、およびマイクロ流路作製用原盤の製造方法
JP2016124263A (ja) * 2015-01-08 2016-07-11 株式会社クラレ 円筒微細構造体の製造方法及びレプリカロールの製造方法

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