WO2023120763A1 - Emulsion-type skin elasticity improving cosmetic material using skin elasticity component material of naturally-derived plant fingerroot, preparation method therefor, and emulsion-type skin elasticity improving cosmetics - Google Patents

Abstract

The present invention relates to a cosmetic material using a natural material-derived extract, and more specifically to: a cosmetic material using a natural plant extract such as a fingerroot root and stem extract, and having excellent skin whitening and skin wrinkle reducing effects; a preparation method therefor; and cosmetics using same.

Description

Emulsion-formulated skin elasticity-improving cosmetic using natural plant finger root's skin-elasticity ingredient, manufacturing method thereof, and emulsion-formulated skin elasticity-improving cosmetics

The present invention relates to an emulsion-type skin elasticity cosmetic with excellent skin whitening and skin wrinkle improvement effects using a finger root rhizome extract from which toxicity has been removed and various natural plant-derived extracts, a method for preparing the same, and an emulsion formulation cosmetic using the same.

Recently, from research on physiological mechanisms through dermatology and research on extracting and refining active ingredients from natural products as new materials with high efficacy, cosmetics that have simply stayed in terms of skin protection have expanded to the category of skin improvement in terms of their efficacy. Demand for functional cosmetics is rapidly increasing.

In particular, among functional cosmetics, multi-functional cosmetics having two or more functionalities in one product (eg, wrinkle-improving function and whitening function at the same time) have a much higher production value and market growth rate than single-functional cosmetics. However, most of the currently marketed cosmetics do not sufficiently satisfy these demands.

Fingerroot (scientific name: Boesenbergia pandurata) is a plant of the ginger family native to Southeast Asia. It is used not only in Asian traditional dishes such as pickles, curry, and drinks, but also as a tonic for childbirth, stomach pain, diarrhea, flatulence, indigestion, ulcer treatment, It has been used for various purposes such as leukocyte prophylaxis and cost supplements.

Fingerroot contains flavonoids that inhibit the infection of Helicobacter pylori bacteria, including indigestion and gastritis, and pinostrobin, which has a cytoprotective effect, increases gastric mucus and reduces the area of gastric ulcer formation. It is also effective in treating peptic ulcer. In addition, it contains components such as flavonoid derivatives, pinocembrin, pinostrobin, ilpinetin, tadamonin, pandurata, and panduratin A that can inhibit the growth of cancer cells. It is known that the component inhibits the growth of cancer cells, and the panduratin A component has a preventive effect on prostate cancer and colon cancer.

According to recent literature, panduratin, a functional raw material extracted from the root of ginger and plants, especially finger root, is a light yellowish brown powder extracted from the root of finger root. and received a grade 2 physiological activity.

Panduratin is a light yellowish brown powder extracted from the root of a plant called finger root. It is known to contribute to reducing body fat by activating 'AMPK (AMP-activated protein kinase)', a sensor protein for maintenance and energy homeostasis in the body.

However, panduratin, one of the finger root extract components, has strong toxicity, so caution is required in use, and there is a limit to its application as a functional cosmetic using it.

The present inventors have developed a method for removing the toxicity of panduratin, a finger root extract that is effective in maintaining skin homeostasis, although it has a slight toxicity disadvantage, as the market demand for multi-functional cosmetics using natural ingredients increases. , By applying the beneficial ingredients of the finger root rhizome extract as a cosmetic using the finger root rhizome extract from which toxicity has been removed as described above, and by applying the mixture with other natural plant-derived extracts, excellent skin whitening, wrinkles, moisturizing, etc. It is an object of the present invention to provide a highly functional cosmetic that can improve skin resistance and skin condition, a manufacturing method thereof, and cosmetics using the same.

In order to solve the above problems, the emulsion-type cosmetic composition for improving skin elasticity of the present invention includes a first liquid, a second liquid, a third liquid and a fourth liquid, wherein the first liquid contains a viscosity modifier and purified water, , The second liquid is a conditioning agent containing at least one selected from glycereth-26, allantoin, hydrolyzed collagen, and adenosine skin; moisturizers including glycerin and butylene glycol; preservatives including 1,2-hexanediol; and a cross-linking agent containing sodium hyaluronate; wherein the third solution is a skin conditioning agent containing at least one selected from sunflower seed oil, dimethicone, cetylethylhexanoate, and glyceryl stearate; surfactants including PEG-100 stearate; humectants including Caprylic/Capric Triglyceride; An emulsifier containing at least one selected from polysorbate 60, stearic acid and sorbitan sesquioleate; and an emulsification stabilizer containing cetearyl alcohol, wherein the fourth liquid is a functional extract containing a pearl extract and a natural plant composite extract; Lactobacillus fermentation lysate; collagen synthesis promoter; And fragrance; contains.

In addition, the present invention relates to a method for preparing the emulsion-type cosmetic for improving skin elasticity, comprising the steps of preparing a first liquid by adding purified water and a viscosity modifier to a reactor and stirring; a second step of injecting the second liquid into the reactor in which the first liquid was prepared and performing stirring; Step 3 of stirring after adding the third liquid to the reactor in which Step 2 was performed; 4th step of adding a pH adjusting agent to the reactor in which step 3 was performed and then stirring; Step 5 of performing agitation after injecting the fourth liquid into the reactor in which Step 4 was performed; Step 6 of filtering and defoaming the mixed solution after step 5; It can be prepared by performing a process including; and step 7 of aging the mixed solution after step 6.

In addition, the present invention relates to a cosmetic for improving skin elasticity using the cosmetic prepared by the method described above, and can provide a cosmetic in an emulsion formulation.

The cosmetic composition of the present invention has excellent skin whitening and skin wrinkle improvement effects while having no skin trouble and excellent moisturizing properties, and thus, it is possible to provide a cosmetic product derived from a highly functional natural material.

1 is a result of measuring the cytotoxicity of the finger root rhizome extract for each solvent conducted in Experimental Example 1.

Figure 2 is the cell protection measurement results of the finger root rhizome extract for each solvent conducted in Experimental Example 2.

Figure 3 is the result of measuring the cell protection effect of the finger root rhizome extract for each solvent conducted in Experimental Example 2.

Figure 4 is a result of measuring the cytotoxicity of each fraction of the finger root rhizome ethanol extract conducted in Experimental Example 3.

Figure 5 is a result of measuring the cell protection effect for each fraction of the finger root rhizome ethanol extract conducted in Experimental Example 4.

6 and 7 are the results of measuring the cytotoxicity and cell protection effect of the water fraction of the finger root rhizome ethanol extract performed in Experimental Example 4.

8 is an evaluation result of intracellular collagen effect on the water fraction of the finger root rhizome ethanol extract conducted in Experimental Example 5.

9 is an evaluation result of the intracellular antioxidant effect on the water fraction of the finger root rhizome ethanol extract conducted in Experimental Example 6.

"Skin improvement" used in the detailed description of the present invention means any action or effect that inhibits or delays skin damage, disease symptoms, improves disease symptoms, or improves the health condition of the skin.

As used in the detailed description of the present invention, "extract" means an active ingredient isolated from a natural product, that is, a substance showing a desired activity. The extract may be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes all forms formulated using the extract or its dry powder. In addition, the extract includes fractionated extracts subjected to the extraction process.

Unless otherwise defined in the detailed description of the present invention, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. there is. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't

Hereinafter, the present invention will be described in more detail through the emulsion-type skin elasticity improving cosmetic of the present invention and a method for preparing the same.

The cosmetic composition of the present invention includes 18 to 22 parts by weight of the second solution, 8 to 12 parts by weight of the third solution, and 10 to 15 parts by weight of the fourth solution, based on 100 parts by weight of the first solution, preferably 100 parts by weight of the first solution. With respect to parts by weight, 18.5 to 21.0 parts by weight of the second solution, 9.0 to 11.5 parts by weight of the third solution and 10.5 to 14.0 parts by weight of the fourth solution, more preferably preferably 100 parts by weight of the first solution, 18.5 to 20.0 parts by weight of the second solution, 10.0 to 11.5 parts by weight of the third solution, and 11.0 to 13.5 parts by weight of the fourth solution.

The first liquid serves as a solvent for cosmetics, and may include 0.20 to 0.40% by weight of a viscosity modifier and the remaining amount of purified water, preferably 0.22 to 0.35% by weight of the viscosity modifier and the remaining amount of purified water. At this time, when the content of the viscosity modifier exceeds 0.40% by weight, the initial viscosity is too high, so that the components of the second to fourth liquids are not properly dissolved in the first liquid, and miscibility of the composition may be poor. In addition, as the viscosity modifier, a general viscosity modifier used in the cosmetics industry may be used, and a preferred example is acrylate/(C _10-30 alkyl)acrylate cross polymer (acrylate/(/(C _10-30 alklyl)acrylate cross polymer) can be used.

Next, among the cosmetic components, the second liquid contains 18.5 to 21.0% by weight of skin conditioning agent, 10 to 12.0% by weight of crosslinking agent, 13.0 to 16.0% by weight of whitening agent, 10 to 15.0% by weight of preservative, and the remainder of the total weight of the second liquid. of moisturizers, preferably 18.8 to 20.5% by weight of skin conditioning agent, 10.2 to 11.7% by weight of crosslinking agent, 13.0 to 15.5% by weight of whitening agent, 12.0 to 15.0% by weight of preservative, and the remainder of the total weight of the second solution of the moisturizer, more preferably 18.8 to 20.0% by weight of the skin conditioning agent, 10.5 to 11.5% by weight of the crosslinking agent, 13.0 to 15.0% by weight of the whitening agent, 13.0 to 15.0% by weight of the preservative and the remaining remaining amount of the moisturizer among the total weight of the second solution can include

Among the components of the second liquid, the skin conditioning agent may include at least one selected from Glycereth-26, Allantoin, Hydrolyzed Collagen, and Adenosine, Preferably, glycereth-26, allantoin, hydrolyzed collagen and adenosine are mixed in a weight ratio of 1:0.1 to 0.4:0.7 to 1.0:0.2 to 0.5, more preferably 1:0.2 to 0.3:0.7 to 0.9:0.2 to 0.4 weight ratio.

Among the components of the second liquid, the crosslinking agent may include sodium hyaluronate, and it is preferable to use a material that does not cause skin trouble as the preservative. For example, 1,2-hexane Diol (1,2-hexane diol) may be included.

In addition, niacinamide may be used as the whitening agent among the components of the second liquid, and the moisturizer may include at least one selected from glycerine and butylene glycol.

Next, among the cosmetic ingredients, the third liquid contains 15.0 to 20.0% by weight of an emulsifier, 5.0 to 8.0% by weight of an emulsion stabilizer, 10.0 to 12.0% by weight of a surfactant, 5.0 to 8.0% by weight of a humectant, and the remaining balance of the total weight of the third liquid. It may include a skin conditioning agent, preferably 16.5 to 20.0% by weight of an emulsifier, 5.5 to 7.5% by weight of an emulsion stabilizer, 10.5 to 12.0% by weight of a surfactant, 5.2 to 7.5% by weight of a humectant, and the remainder of the total weight of the third solution. of skin conditioning agents.

Among the components of the third liquid, the emulsifier serves to increase the miscibility between cosmetic compositions and the emulsification of the composition, and includes polysorbate 60, stearic acid, and sorbitan sesquioleate (Sorbitan sesquioleate), preferably polysorbate 60, stearic acid and sorbitan sesquioleate in a weight ratio of 1: 0.5 to 1.0: 0.5 to 1.0, more preferably polysorbate 60, stearic acid and sorbitan sesquioleate are mixed and used in a weight ratio of 1: 0.8 to 1.0: 0.5 to 0.9.

Among the components of the third liquid, general emulsion stabilizers used in the art may be used as the emulsion stabilizer, and preferably, cetearyl alcohol is used.

In addition, it is preferable to use PEG-(40-100) stearate, preferably PEG-100 stearate, as the surfactant among the components of the third liquid.

In addition, the humectant among the components of the third liquid is caprylic acid, caprylic acid, and caprylic / capric triglyceride, which is a mixed triester of capric acid and glycerin. Triglycerides) are recommended.

In addition, the skin conditioning agent among the components of the third liquid may include at least two selected from sunflower seed oil, dimethicone, cetyl ethyl hexanoate, and glyceryl stearate. Preferably, sunflower seed oil, dimethicone, cetylethylhexanoate, and glyceryl stearate may be included in a weight ratio of 1:0.8 to 1.0:0.5 to 0.8:0.4 to 0.8.

Next, among the cosmetic ingredients, the fourth liquid contains 5.0 to 8.0% by weight of the lactobacillus fermentation lysate, 1.0 to 2.5% by weight of the collagen synthesis promoter, 0.1 to 0.8% by weight of the fragrance, and the remaining balance of the functional extract of the total weight of the fourth liquid. 5.5 to 7.0% by weight of the lactobacillus fermented lysate, 1.2 to 2.3% by weight of the collagen synthesis promoter, 0.1 to 0.8% by weight of the fragrance, and the remaining functional extract of the total weight of the fourth solution. there is. At this time, the lactobacillus fermented lysate among the components of the fourth liquid serves to promote the aging of the functional extract, and if the content thereof is less than 5.0% by weight, the functional extract may not mature well and cause skin trouble. Excessive use is uneconomical.

In addition, the collagen synthesis accelerator is absorbed into the skin and serves to promote collagen synthesis in the skin, and RH-Oligopeptide-1 may be used. In addition, if the content of the collagen synthesis promoter is less than 1.0% by weight, the amount used is too small and the effect of promoting collagen synthesis in the skin is insufficient, so the effect of improving skin wrinkles may be insufficient. It is recommended to use it within the above range because it may interfere with the

In addition, as for the type of fragrance, general fragrances used in the art may be used as long as they do not cause skin trouble or impair the function of the cosmetic of the present invention.

In addition, the functional extract includes a pearl extract and a natural plant complex extract, the pearl extract and the natural plant complex extract in a weight ratio of 1: 3.5 to 5.0, preferably the pearl extract and the natural plant complex extract in a weight ratio of 1: 3.8 to 4.8 can be included with

The pearl of the pearl extract refers to a bead-shaped secretion mass formed in the body of shellfish. After pulverizing 100 g of pearls, 2 to 4 L of 60 to 80 vol% ethanol aqueous solution is used as a solvent for 10 to 16 hours. 1st step of reflux extraction with moderate heating; A second step of cooling the reflux extract and then filtering with a filter paper to obtain a filtrate; It can be prepared by carrying out a process including three steps of concentrating the obtained filtrate by vacuum concentration and vacuum drying to obtain pearl dry powder.

In addition, the natural plant complex extract is finger root rhizome extract (Boesenbergia Pandurata Rhizome Extract), centella extract, vitamin tree extract (Hippophae Rhamnoides Extract), green tea extract (Camellia Sinensis Leaf Extract), Matricaria extract (Chamomilla Recutita Extract) , Opuntia Humifusa Extract, Morus Alba Leaf Extract, Crataegus Pinnatifida Fruit Extract, Mentha Piperita (Peppermint) Extract, Hibiscus Sabdariffa Flower Extract ), rosemary extract (Rosmarinus Officinalis (Rosemary) Extract), quince extract (Chaenomeles Sinensis Fruit Extract) and red chokeberry extract (Aronia Arbutifolia Extract), preferably with respect to 100 parts by weight of finger root rhizome extract, Centella asiatica Extract 15 ~ 30 parts by weight, vitamin tree extract 0.2 ~ 2.0 parts by weight, green tea extract 0.2 ~ 2.0 parts by weight, Matricaria 0.2 ~ 2.0 parts by weight, low tanseon extract 0.1 ~ 1.0 parts by weight, mulberry leaf extract 0.1 ~ 1.0 parts by weight , hawthorn fruit extract 0.1 ~ 1.0 parts by weight, peppermint extract 0.1 ~ 1.0 parts by weight, hibiscus flower extract 0.1 ~ 1.0 parts by weight, rosemary extract 0.1 ~ 1.0 parts by weight, quince extract 0.1 ~ 1.0 parts by weight and red chokeberry extract 0.1 ~ 1.0 parts by weight 1.0 parts by weight, more preferably 100 parts by weight of finger root rhizome extract, 15 to 25 parts by weight of centella asiatica extract, 0.3 to 1.5 parts by weight of vitamin tree extract, 0.3 to 1.5 parts by weight of green tea extract, Matrica Rhea extract 0.3 ~ 1.5 parts by weight, low tanning extract 0.1 ~ 0.8 parts by weight, mulberry leaf extract 0.1 ~ 0.8 parts by weight, hawthorn fruit extract 0.1 ~ 0.8 parts by weight, peppermint extract 0.1 ~ 0.8 parts by weight, hibiscus flower extract 0.1 ~ 0.8 parts by weight It is preferable to include 0.1 to 0.8 parts by weight of rosemary extract, 0.1 to 0.8 parts by weight of quince extract and 0.1 to 0.8 parts by weight of red chokeberry extract.

The finger root rhizome extract is an extract from which toxicity has been removed, and it is recommended to use a finger root rhizome ethanol extract or a water fraction of the finger root rhizome ethanol extract, preferably a finger root rhizome ethanol extract from which toxicity has been removed. It is preferable to use the water fraction, which can be prepared through the following method.

The water fraction of the finger root rhizome ethanol extract is obtained by mixing the finger root rhizome powder with an aqueous ethanol solution, stirring, and performing an extraction process at 15 to 30 ° C for 18 to 24 hours to obtain an ethanol extract. Step 1 to obtain; A second step of filtering the ethanol extract, adding distilled water, and then concentrating in a vacuum to obtain a concentrated solution from which ethanol is removed; And after re-dissolving the concentrate in DMSO (dimethyl sulfoxide), it can be prepared by performing a process comprising: 3 steps of filtering with a sterile filter and then freeze-drying.

And, in the water fraction manufacturing process, the 3-step step 3-1 of removing the lower layer solution after adding dichloroform to the concentrated solution, stirring and leaving it to stand; And after removing the lower layer liquid, ethyl acetate (Ethyl Acetate) is added and left to stir to obtain a lower layer liquid, and then 3-2 steps of lyophilizing and powdering the obtained product.

Hereinafter, a method for preparing a cosmetic composition containing the first to fourth liquids described above will be described.

The cosmetic composition of the present invention includes a first step of preparing a first liquid by adding purified water and a viscosity modifier to a reactor and stirring; a second step of injecting the second liquid into the reactor in which the first liquid was prepared and performing stirring; Step 3 of stirring after adding the third liquid to the reactor in which Step 2 was performed; 4th step of adding a pH adjusting agent to the reactor in which step 3 was performed and then stirring; Step 5 of performing agitation after injecting the fourth liquid into the reactor in which Step 4 was performed; Step 6 of filtering and defoaming the mixed solution after step 5; It can be prepared by performing a process including; and step 7 of aging the mixed solution after step 6.

The stirring in the first step is performed at 60 to 80 ° C and a stirring speed of 2500 to 3500 rpm, preferably for 40 to 80 minutes, preferably for 50 to 70 minutes, under the conditions of 65 to 75 ° C and a stirring speed of 2800 to 3200 rpm. good to do At this time, if the temperature during stirring is less than 60 ° C or the stirring speed is less than 2500 rpm, the viscosity modifier may not be completely dissolved in the purified water, so there may be a problem of lengthening the stirring time, and it is uneconomical when the temperature exceeds 80 ° C during stirring. , If the stirring speed exceeds 3500 rpm, bubbles may be generated, so it is good to perform stirring under the above conditions.

The stirring in the second step is performed at 60 to 80 ° C and a stirring speed of 2000 to 3000 rpm, preferably for 40 to 80 minutes, preferably for 50 to 70 minutes, under the conditions of 65 to 75 ° C and a stirring speed of 2200 to 2700 rpm. good to do

In addition, the stirring in the third step is performed at 70 to 80 ° C and a stirring speed of 2000 to 3000 rpm, preferably at 65 to 75 ° C and a stirring speed of 2200 to 2700 rpm for 30 to 60 minutes, preferably 30 to 60 ° C. It is better to do it for minutes.

And, step 4 is a process of performing pH adjustment before mixing the fourth liquid in step 5, and after lowering the temperature in the reactor in which step 3 was performed, a pH adjuster is introduced and stirring is performed. At this time, stirring is carried out for 10 to 30 minutes, preferably for 20 to 30 minutes under the conditions of 35 ~ 45 ℃ and stirring speed 2000 ~ 3000 rpm, preferably under the conditions of 35 ~ 40 ℃ and stirring speed 2200 ~ 2700 rpm It is good. In addition, arginine may be used as a preferred example of the pH adjusting agent, and it is appropriate to add 0.10 to 0.50 parts by weight, preferably 0.20 to 0.40 parts by weight, based on 100 parts by weight of the first solution.

And, the stirring in step 5 is performed at 35 to 45 ° C and a stirring speed of 2000 to 3000 rpm, preferably at 35 to 40 ° C and a stirring speed of 2200 to 2700 rpm, for 10 to 30 minutes, preferably for 20 to 30 minutes. It is good to do it while

The cosmetic composition for improving skin elasticity of the present invention prepared by the composition and method described above can be prepared as a cosmetic of any formulation conventionally manufactured in the art, and is commonly used in the field of dermatology by containing a dermatologically acceptable medium or base. It can be prepared in the form of an adjuvant that can be applied topically or systemically.

In addition, preferably, it can be prepared as an emulsion-type cosmetic, and the emulsion-type cosmetic may have a viscosity of 45,000 to 51,000 cP and a pH of 6.0 to 7.0.

In the above, the present invention has been described with a focus on embodiments, but this is only an example and does not limit the embodiments of the present invention, and those skilled in the art to which the embodiments of the present invention belong will appreciate the essential characteristics of the present invention. It will be appreciated that various modifications and applications not exemplified above are possible within a range that does not deviate. For example, each component specifically shown in the embodiment of the present invention can be modified and implemented. And differences related to these modifications and applications should be construed as being included in the scope of the present invention as defined in the appended claims.

[Example]

Preparation Example 1: Preparation of finger root rhizome extract

(1) After taking 5 g of finger root rhizome powder, 100 ml of hexane, dichloroform, ethyl acetate, ethanol, methanol and water for each solvent Then, extracts were obtained by extraction at 25° C. for 24 hours using a shaker. Next, the obtained extract was filtered using a filter (Whatman No.2 filter paper), and then the extract for each solvent was concentrated in a vacuum. The concentrate was re-dissolved in DMSO (dimethyl sulfoxide, dimethyl sulfoxide), filtered through a 0.22um sterile filter, and stored frozen at -20°C. Then, the water extract was filtered and lyophilized, and the powder was re-dissolved in 50 vol% DMSO and filtered through a sterile filter before use.

Dichloroform, ethyl acetate, ethanol, and methanol solvent extracts showed a light yellow color, and hexane extracts showed colorless.

(2) Indicator component analysis

Finger root rhizome extracts were aliquoted at the same concentration and prepared as HLPC samples.

The panduratin A content in the extract was analyzed by comparing the HPLC chromatogram of the index component panduratin A of the finger root extract and the extract for each solvent. The chemical structure of panduratin A is shown in Formula 1 below.

[Formula 1]

As for the analysis method, panduratin A was dissolved in methanol and diluted in stages to prepare samples for quantitative writing. The HPLC measurement conditions are shown in Table 1 below, and the validation of the analysis method is shown in Table 2 below.

item	analysis conditions
Detector	UV 280 nm
Column	C 18 (4.6mm ID × 250mm, 5μm)
mobile phase (Mobile phase)	time (minutes)	A[DW] (%)	B [methanol] (%)
	5	40	60
	10	0	100
	15	0	100
	25	40	60
	30	40	60
column temperature	55℃
injection volume	20μL
flux	1mL/min

specificity	Weigh accurately about 100 mg of Panduratin A and dissolve it in dilution solution to make it standard solution (about 1 000 mg/kg) in 100 mL. Then, this standard solution is diluted with dilution solution to analyze Panduratin A standard solution and dilution solution without Panduratin A. and compared with the analysis results of the standard solution.
linearity	Anduratin A standards were analyzed at 4 concentrations, and the coefficient of determination (r2) of linearity was evaluated. A standard solution was prepared by diluting the prepared standard solution (about 1,000 mg/kg) with MeOH.
range	When preparing a calibration curve for Panduratin A in the validation test method, the concentration of the standard solution for which linearity is proven is 1 mg/kg, 5 mg/kg, and 10 mg/kg in consideration of the accuracy test concentration and precision test concentration. , and the concentration range was set at 50 mg/kg.
accuracy (recovery rate)	After adding a certain amount by concentration using Panduratin A standard stock solution (1,000 mg/kg) to MeOH without Panduratin A, MeOH was added and dispersed by ultrasonic wave for 15 minutes. After filtering (0.20 μl syringe filter), it was used as the test solution. After repeating the measurement three times independently for each concentration, the recovery rate was confirmed and evaluated.
precision (reproducibility)	After adding 0.5 mL of Panduratin A standard stock solution (1 000 mg/kg) to MeOH without Panduratin A, add 5 mL of 10% MeOH, disperse by ultrasonic wave for 15 minutes, apply 10 mL, and filter (0.20 μl syringe). After filter), it was used as a test solution. The concentration of Panduratin A 50 mg/kg) was measured 7 times, and the system suitability was confirmed by the relative standard deviation of the resultant value.
Limits of detection and quantification	Approximately 100 mg of the Panduratin A standard was precisely weighed and dissolved in the mobile phase, and the standard stock solution (approximately 1,000 mg/kg) was used as a standard stock solution (approximately 1,000 mg/kg) in 100 mL. Limits of quantification were calculated.

In addition, the results of measuring the content of panduratin A in the extract for each solvent are shown in Table 3 below.

extraction solvent	Retention time	Area	Concentration (ppm)
Panduratin A 1 ppm	29.377	6133	1.348
Panduratin A 5 ppm	29.379	40774	4.660
Panduratin A 10 ppm	29.380	96179	9.956
Panduratin A 50 ppm	29.365	515463	50.036
hexane	29.197	3364	1.088
ethyl acetate	29.346	34382	4.042
dichloroform	29.336	7134	1.437
methanol	29.364	30281	3.663
ethanol	29.362	34237	4.030

Looking at the measurement results in Table 3, the indicator substance panduratin A was detected at 29.3 minutes in all extraction solvents, and ethyl acetate (4.042), ethanol (4.030), and methanol (3.663) contained high concentrations of panduratin A in that order. I was able to confirm that Experimental Example 1: Toxicity test of finger root rhizome extract

A toxicity test was performed on each of the nucleic acid extract (H), dichloroform extract (Ch), ethyl acetate extract (EA), ethanol extract (Et), methanol extract (Me), and water extract prepared in Preparation Example 1, The results are shown in A to C of FIG. 1 . AsA used in the test is labeled with ascorbic acid (Vit.C) as a positive control.

Extracts using nucleic acid (hexane, H), dichloroform (Ch), ethyl acetate (EA), ethanol (ethanol, Et), methanol (Me), and water (water, W) as solvents, respectively As a result of treating skin cell Hs68 at concentrations of 0.1, 0.5, 1, and 5 μg/ml, high toxicity was observed in all extracts except for the water extract at 5 μg/ml.

Experimental Example 2: Cell protection effect of finger root rhizome extract against UVB stimulation and inhibition of collagenase production

(1) Cell protective effect

In order to measure the cytoprotective effect on UVB stimulation, the experimental results of measuring the cytoprotective effect on UVB stimulation of each solvent were measured at the same concentration as when measuring cytotoxicity, A to C in FIG. shown in As shown in FIG. 2, the cell protection effect was not evident in the samples other than ethanol and methanol.

In addition, A and B of Figure 3 are graphs showing the results of experiments with concentrations of 0.5 and 1 μg / ml in order to prepare for the cell protection effect of each solvent finger root rhizome extract against UVB stimulation. As shown in Figure 3, as a result of the cell protection effect test for UVB stimulation, the effect was the best in the ethanol extract, and the protective effect was significantly increased at 0.5 μg / ml than at 1 μg / ml, and the finger root root The cell protection effect of the stem ethanol extract against UVB was the highest.

(2) Collagenase production inhibition experiment

Collagenase production (Pro-collagen synthesis) inhibitory efficacy test was performed at 0.5 μg / ml, 1.0 μg / ml, and 2.5 μg / ml of finger root rhizome ethanol extract, and the results are shown in Table 4 below. The positive control group was TFG-beta1 100ng/ml, and the control group was an ethane water extract untreated group.

division	MMP-1 production (% compared to control)
control group	100%
positive control	63.5%
Ethanol extract 0.5μg/ml	75.6%
Ethanol extract 1.0μg/ml	70.8%
Ethanol extract 2.5μg/ml	63.3%

Through the collagenase production inhibition experiment in Table 4, it was confirmed that the ethanol extract of the finger root rhizome has an inhibitory effect on collagenase activity, and through this, there is an active effect on improving skin wrinkles.

Preparation Example 2: Preparation of fractions of finger root rhizome extract

100 g of finger root rhizome powder was added to 1 L of ethanol and extracted at 25° C. for 24 hours using a shaker. Finger root rhizome ethanol extract was obtained by filtering using a filter (Whatman No.2 filter paper). 100 ml of 1 L of finger root rhizome ethanol extract was taken and 200 ml of distilled water was added.

A mixture of finger root rhizome ethanol extract and distilled water was concentrated in vacuo to remove all ethanol, and then transferred to a separatory funnel. A total of 2 L of dichloroform was added thereto, sufficiently shaken, and then separated, and the lower layer was collected and concentrated.

After the dichloroform fractionation, a total of 1 L of ethyl acetate was added, sufficiently shaken, separated, and the supernatant was collected and concentrated. Ethyl acetate was removed and the remaining water layer was lyophilized to form a powder. Both concentrate and powder were re-dissolved in DMSO, then filtered through a 0.22um sterile filter, and stored at -20 ° C until analysis, ethanol fraction, dichloroform fraction, ethyl acetate fraction and water fraction for finger root rhizome ethanol extract were respectively obtained.

Experimental Example 3: Cytotoxicity test of ethanol-extracted fractions of finger root rhizome

Cytotoxicity was performed on the ethanol-extracted fraction obtained in Preparation Example 2, and cell culture and cytotoxicity experiments were as follows.

Hs68 cells, a human skin fibroblast cell line used in the cytotoxicity test, were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA). DMEM (Dulbecco's Modified Eagle's Medium) medium containing 10% FBS, penicillin (100 units/ml), and streptomycin (50 μg/ml) was used at 37°C, 5% carbon dioxide (CO2) and 95% humid air ( humid air) and cultured in an incubator (Thermo Scientific, Tewksbury, MA, USA). Hs68 cells were dispensed in a 96-well plate at a concentration of 1.0×10 4 cells/well, and after culture for 24 hours, the medium was replaced with a medium containing the sample and not containing FBS. After 24 hours of sample treatment, 20uL of MTT (1 mg/ml) solution was added to each well and incubated for 2 hours. Finally, the supernatant was removed, and blue formazan crystals generated from viable cells were solubilized with DMSO, and absorbance at 550 nm was measured using a Microplate reader (BioTek, Inc., Winooski, VT, USA). During sample processing, the sample was diluted with medium so that the final concentration of DMSO was less than 0.1% (v/v).

To evaluate the cytotoxicity according to the concentration of each fraction, hs68 cells were treated with 0.1, 0.5, 1, or 5 μg/ml. As a result of treatment by concentration, as shown in A and B of FIG. 4, all of the fractions except the water fraction showed cytotoxicity at 5 μg/ml.

Experimental Example 4: Cell protection effect test for UVB stimulation of ethanol-extracted fractions of finger root rhizome

Hs68 cells were dispensed in a 96-well plate at a concentration of 1.0 × 10 ⁴ cells/well and cultured for 24 hours, and then the samples were diluted with FBS-free medium in each well, treated, and cultured for 24 hours.

Then, UVB (30mJ/cm ² ) was irradiated using a UVB lamp (Sankyo Denki Lamps, GL20SE, Marine, Japan), and ultraviolet intensity was monitored using a UV LIGHT METER (LT Lutron, UV-340A, Taiwan). . All UVB irradiation was performed after thinly applying PBS to the cells attached to the 96-well plate. After irradiation, the samples were diluted in FBS-free medium and treated with cells for 24 hours. The control group was run under the same conditions without UVB irradiation.

In order to evaluate the cell protection effect on UVB irradiation for each of the ethanol-extracted fractions, the extracts for each solvent were treated at concentrations of 0.1, 0.5, 1, and 5 μg/ml before and after UVB irradiation on the cells. As a result, as shown in A and B of FIG. 5, only the water fraction maintained the protective effect activity without toxicity up to 5 μg/ml.

The water fraction of the finger root rhizome ethanol extract was selected and the cytotoxicity and cell protection effect against UVB at each concentration (1, 5, 25, 50 μg/ml) were measured, and the results are shown in FIG. 6 . Looking at the cytotoxicity test results of FIG. 6A, in the case of cytotoxicity, cytotoxicity was not shown up to 25 μg / ml, but at 50 μg / ml, the cell viability was reduced to 86%, which was determined to indicate toxicity. On the other hand, in the case of the cell protection effect, as shown in B of FIG. 6, which is the result of the cell protection experiment, the cell protection effect was shown at 5 μg/ml and 25 μg/ml.

As a result of the above test, the cell viability tended to decrease slightly at 25μg/ml of the water fraction of the finger root rhizome ethanol extract. Therefore, the highest concentration was set at 20μg/ml in order to obtain an ideal result that increases in a concentration-dependent manner during sample treatment did

As a result of measuring the cytotoxicity and cell protection effect against UVB of 1 to 20 μg/ml of the water fraction of the finger root rhizome ethanol extract, no toxicity was shown up to 20 μg/ml (see A in FIG. 7), and the UVB-induced Cell viability was increased in a concentration dependent manner (see B in FIG. 7 ).

Experimental Example 5: Evaluation of Intracellular Collagen Effect on Water Fraction of Finger Root Rhizome Ethanol Extract

UVB irradiation reduces cell collagen production. Therefore, the effect of the water fraction of the fingerroot extract on the amount of intracellular collagen was measured. The experimental method is as follows.

(1) Measurement of MMP-1, MMP-3 and water-soluble collagen production

Measurement of MMP-1 and MMP-3, whose synthesis is increased by UVB irradiation, was performed using enzyme-linked immunosorbents using the human MMP-1 and MMP-3 ELISA kit (Merck & Co. Inc., Whitehouse Station, NJ, USA). The production amount of pro-MMP-1 and MMP-3 in the medium was measured by the assay method. After UVB irradiation, the amount of soluble collagen produced was measured using SircolTM soluble collagen assay kit. The medium in which the cells were cultured was collected, mixed with and reacted with Sirco dye reagent, centrifuged, and then cold acid-salt washing reagent was added to the precipitate and mixed. After centrifuging the mixture, the precipitate was dissolved using an alkali reagent, and the absorbance was measured at 555 nm, and the experimental results are shown in FIG. 8A.

Looking at A of FIG. 8, UVB decreased the amount of intracellular collagen, and the collagen level increased in a concentration-dependent manner as the concentration increased when the water fraction was treated from 1 μg/ml to 20 μg/ml.

At this time, UVB irradiation promotes the decomposition of collagen by increasing the production of MMP-1 (Matrix MetalloProtease), an enzyme that decomposes type 1 collagen, which is the most abundant protein in the skin. Therefore, the effect of the water fraction of the fingerroot extract on the production of MMP-1 was evaluated by the method described above, and the results are shown in FIG. 8B. The production of MMP-1 increased upon UVB irradiation, and the production of MMP-1 decreased as the concentration increased from 1 μg/ml to 20 μg/ml when the water fraction was treated.

In addition, since MMP-3 promotes collagen degradation by activating MMP-1, the amount of MMP-3 produced was measured in the same manner as in MMP-1, and the experimental results are shown in FIG. 8C. The production of MMP-3 increased upon UVB irradiation, and the production of MMP-3 decreased as the concentration increased from 1 μg/ml to 20 μg/ml when the water fraction was treated.

Experimental Example 6: Evaluation of intracellular antioxidant effect on the water fraction of finger root rhizome ethanol extract

UVB irradiation promotes the generation of intracellular reactive oxygen species (ROS). The increase in these abnormal reactive oxygen species induces oxidative stress and induces MMPs in dermal fibroblasts to promote skin aging. Therefore, the effect of the water fraction of the finger root rhizome extract on ROS production was evaluated. The experimental method is as follows.

(1) Measurement of ROS generation

To measure the production of reactive oxygen species (ROS), Hs68 cells were pretreated with samples for 24 hours, washed with PBS, and then irradiated with UVB (30 mJ/cm2). After UVB irradiation, the cells were treated with samples for 30 minutes, and then stained with 25uM DCFH-DA. Fluorescence intensity corresponding to intracellular ROS generation was measured with a fluorescent spectrophotometer (Perkin-Elmer, Norwalk, CT, USA) for 2 hours at an excitation wavelength of 485 nm and an emission wavelength of 530 nm.

9 shows a graph of fluorescence intensity measurement for measuring ROS generation of reactive oxygen species (ROS) according to concentrations of 1 to 20 μg/ml of the water fraction of the finger root ethanol extract.

Referring to FIG. 9 , it was confirmed that UVB irradiation increased intracellular ROS generation, and decreased ROS generation in a concentration-dependent manner when the water fraction was treated.

Example 1: Preparation of skin elasticity improving cosmetics (cosmetics) of emulsion formulation

Purified water and acrylate/C _10-30 alkyl acrylate crosspolymer (Carbopol ETD-2020) 0.28% by weight and 99.72% by weight of purified water were added, and then stirred at 3,000 rpm for 60 minutes at 70 ° C to prepare a first solution.

After preparing a second liquid containing a skin conditioning agent, a moisturizer, a preservative, and a crosslinking agent, 19.0 parts by weight of the second liquid based on 100 parts by weight of the first liquid was introduced into the reactor where the first liquid was prepared, and then 2,500 rpm at 70 ° C. Stirring was performed for 60 minutes at speed. At this time, the composition of the second liquid is shown in Table 5 below.

2nd liquid	ingredient	weight%
moisturizer	glycerin	25.7
	Butylene Glycol	14.7
whitening agent	Niacinamide	14.7
preservative	1,2-Hexanediol	14.7
cross-linking agent	sodium hyaluronate	11
skin conditioning agent	glycereth-26	9.5
	allantoin	2.2
	hydrolyzed collagen	7.2
	adenosine	0.3
total		100

Next, after preparing a third liquid containing a skin conditioning agent, surfactant, moisturizer, emulsifier, and emulsion stabilizer, 10.8 parts by weight of the third liquid based on 100 parts by weight of the first liquid is introduced into the reactor in which the second liquid is mixed. After that, stirring was performed at 2,500 rpm for 50 minutes at 75 °C. At this time, the composition of the third liquid is shown in Table 6 below.

3rd liquid	ingredient	weight%
skin conditioning agent	sunflower seed oil	16.8
	dimethicone	15.5
	Cetylethylhexanoate	12.9
	Glyceryl Stearate	12.3
Surfactants	PEG-100 Stearate	11.5
moisturizer	Caprylic/Capric Triglyceride	6.5
emulsion stabilizer	Cetearyl Alcohol	6.5
emulsifier		Polysorbate 60	6.5
	Stearic Acid	6.5
	Sorbitan Sesquioleate	5
total		100

Next, after lowering the temperature of the reactor in which the third liquid was mixed to 40 ° C, 0.28 parts by weight of arginine as a pH adjuster was added to 100 parts by weight of the first liquid, and then 30 at 2,000 rpm at 40 ° C. Next, a fourth liquid containing functional extracts including pearl extract and natural plant complex extract, Lactobacillus fermentation lysate, collagen synthesis promoter and flavoring was prepared in a reactor in which arginine was mixed. , 11.6 parts by weight of the fourth liquid based on 100 parts by weight of the first liquid was introduced into the reactor in which the pH adjusting agent was mixed, and stirring was performed at 40 ° C. at a speed of 2,000 rpm for 30 minutes. At this time, the composition of the fourth liquid is shown in Table 7 below.

4th liquid	ingredient	weight%
functional extract	pearl extract	18
	Natural Plant Complex Extract	73.8
Lactobacillus fermentation lysate		6
collagen synthesis promoter	RH-Oligopeptide-1	1.8
Spices		0.4
total		100

At this time, the pearl extract was pulverized with 100 g of pearls, extracted under reflux by heating for 12 hours using 3 L of 70% ethanol aqueous solution as a solvent, cooled, and filtered with a filter paper having a 1.2 μm permeation size to obtain a filtrate, The obtained filtrate was concentrated under reduced pressure and dried under reduced pressure, and then dried and pulverized to be used. In addition, the finger root rhizome extract is the water fraction of the ethanol extract, and is prepared by the following process. The water fraction of the stem ethanol extract was used.

100 g of finger root rhizome powder was added to 1 L of ethanol and extracted at 25° C. for 24 hours using a shaker. Next, the finger root rhizome ethanol extract was obtained by filtering using a filter (Whatman No.2 filter paper). 100 ml of 1 L of finger root rhizome ethanol extract was taken and 200 ml of distilled water was added. Next, the mixture of the finger root rhizome ethanol extract and distilled water was concentrated in a vacuum to remove all ethanol, and then transferred to a separatory funnel. A total of 2 L of dichloroform was added thereto, sufficiently shaken, and then separated, and the lower layer was collected and concentrated. Next, after the dichloroform fractionation, a total of 1 L of ethyl acetate was added, shaken sufficiently, and the supernatant was separated, and the lower layer was lyophilized to obtain a water fraction, which was then freeze-dried to obtain an ethanol extract of finger root rhizome A dry powder of the water fraction was obtained.

In addition, the composition ratio of the natural plant composite extract is shown in Table 8 below, and other plant extracts other than the finger root rhizome extract were purchased commercially.

Natural plant complex extract	parts by weight
Finger root rhizome extract	100
centella asiatica extract	20
Vitamin Tree Extract	0.4
green tea extract	0.4
Matricaria Extract	0.4
low sweetness extract	0.2
Mulberry Leaf Extract	0.2
Hawthorn Fruit Extract	0.2
Peppermint Extract	0.2
hibiscus flower extract	0.2
rosemary extract	0.2
Chinese quince extract	0.2
Red Chokeberry Extract	0.2

Next, the solution prepared by mixing and stirring the fourth liquid was filtered and degassed, and then aged to finally prepare cosmetics. The prepared cosmetic was an emulsion formulation, and had a viscosity of 47,000 to 49,000 cP and a pH of about 6.4 to 6.6 at 25°C. Comparative Example 1: Preparation of skin elasticity cosmetics

Korean Patent No. 10-2288066 discloses a first mixture (emulsion agent, emulsifier, flavoring agent), a second mixture (purified water, adenosine, niacinamide) and a third mixture (natural plant-derived extract, natural plant-derived fermented filtrate, Conditioning agent, stabilizer, preservative, sequestering agent) were prepared, respectively, and skin elasticity cosmetics were prepared through the following process.

In Table 9, the conditioning agents of the third mixture include Acetyl Hexapeptide-8, Copper Tripeptide-1, sh-Oligopeptide-10, Butylene Gly It was prepared by mixing Butylene Glycol, Propylene Glycol, Hyaluronic Acid, and Methyl Perfluoroisobutyl Ether in equal weight ratios. .

After putting, dispersing, dissolving, and mixing the first mixture into the first container (step 11), the second mixture was dispersed, dissolved, and mixed (step 21). Next, the third mixture was dispersed, dissolved, and mixed. Mixed (step 31). The first mixture mixed in step 11, the second mixture mixed in step 21, and the third mixture mixed in step 31 were each transferred to an emulsifier and mixed and stirred in an emulsifier or the like in step 32. In addition, the mixed and stirred mixture in step 32 was filtered (step 33), vacuum degassed (step 34), and then transferred to a storage tank to perform storage and aging (step 35) to prepare skin elasticity cosmetics. The prepared cosmetic was put into a container and stored.

division	ingredient		Content (% by weight)
1st mixture (10)	emulsifier		polysorbate 80	4.0
	softener	Cyclopentasiloxane	7.5
	flavoring agent	Mixed Spices	0.25
2nd mixture (20)	Purified water		12.5
	adenosine		0.08
	Niacinamide		2.5
3rd mixture (30)	Fingerroot Root Stem Extract		4.0
	centella extract		2.5
	purslane extract		2.5
	mung bean extract		1.0
	Eucalyptus Leaf Extract		1.0
	Western Lion Extract		1.0
	mud mushroom extract		0.5
	Matricaria Extract		0.5
	Virginia Plum Blossom Extract		2.5
	collagen extract		2.5
	Lotus Callus Culture Extract		1.2
	Mulberry Bark Extract		1.2
	BAMBUSA VULGARIS EXTRACT		0.5
	bifida fermentation filtrate		7.5
	Galactomyces fermentation filtrate		2.5
	Yeast/cordyceps ferment filtrate		2.5
	conditioning agent		36.57
	stabilizator	Hydroxyethyl Cellulose	0.7
	preservative	1,2-Hexanediol	2.0
	sequestering agent	Disodium EDT	0.5
Total weight %			100

Experimental Example 7: Measuring the wrinkle improvement effect around the eyes

The effect of improving wrinkles around the eyes before and after using the cosmetics of Example 1 and Comparative Example 1 was measured, and wrinkles around the eyes were measured using PRIMOS (Phaseshift rapid in-vivo measurement of skin), and the evaluation results are shown in Table 10 below. Ra and R3z were selected as PRIMOS analysis variables, and the variable Ra (arithmetic roughness average) is the arithmetic average value of the peak of the roughness section of wrinkles measured by PRIMOS. means improved, and the unit is μm.

The variable R3z (Base roughness depth) is expressed as the arithmetic average of five single roughness depths from R3z1 to R3z5. The single roughness depth means the vertical distance between the third highest cross section peak and the third deepest curve at a single evaluation distance (Ir) of the roughness cross section. As the value of R3z decreases, it means that the depth of wrinkles in the skin decreases, which means that wrinkles are improved. Unit is μm. The temporary wrinkle improvement rate was calculated according to Equation 1 below.

[Equation 1]

Periorbital wrinkle improvement rate (%) = 100% - {(A-B)/B}×100%

In Equation 1, A is a measured value after using the product, and B is a measured value before using the product.

division	Ra		R3z
	Ra measurements	Periorbital wrinkle improvement rate (Ra)	R3z measurements	Periorbital wrinkle improvement rate (R3z)
before use	15.869	100%	42.237	100%
Comparative Example 1	15.015	105.38%	40.250	104.70%
Example 1	14.990	105.54%	40.122	105.00%

As a result of analyzing the wrinkles around the eyes, both the variables Ra and R3z showed a statistically significant temporary improvement in wrinkles around the eyes (p<0.05) after use compared to before and after use of the cosmetics of Comparative Example 1 and Example 1 (p<0.05), Comparative Example Example 1 showed a relatively better improvement rate of wrinkles around the eyes than Example 1.

Experimental Example 8: Measurement of skin elasticity improvement effect

The skin elasticity improvement effect was measured before and after using the cosmetics of Example 1 and Comparative Example 1, and the test site was measured three times using a Cutometer (Courage and Khazaka Electronic, Germany), and the average value was obtained using the three values. The measurement site was measured at a point 3 cm away from the outer corner of the eye. Cutometer applies negative pressure to the skin surface and measures the degree to which the skin is sucked into the probe using an optical system that can pass through the skin. The light used at this time is infrared, and the degree of elasticity is measured by the ratio of the intensity of light that is reduced while passing through the skin and the intensity of light that does not pass through the skin. R2 (A.U.) was selected as a cutometer measurement variable for elasticity.

Variable R2 represents the ratio of the total contraction length of the skin at the maximum value at which the skin can be stretched, and represents the overall elasticity, which is the re-deformation force of the skin. The closer the value of this variable is to 1, the more elastic it is. The skin elasticity improvement rate was calculated according to Equation 1 below. Measurements were taken before use (0 weeks), after 2 weeks and after 4 weeks of use.

During the evaluation period, adverse skin events caused by the test product did not occur, and the skin elasticity measurement results are shown in Table 11 below.

[Equation 2]

Skin elasticity improvement rate (%) = {(B-A)/B}×100%

In Equation 2, A is the R2 measured value after product use, and B is the R2 measured value before product use.

division	0 weeks R2 measured value	2 weeks later R2 measured value	4 weeks later R2 measured value	4 weeks later Skin elasticity improvement rate (%)
Comparative Example 1	0.7703	0.7767	0.7785	101.06
Example 1	0.7712	0.7782	0.7813	101.23

As a result of analyzing the wrinkles around the eyes, both the cosmetics of Comparative Example 1 and Example 1 showed an effect of improving skin elasticity after use compared to before use (p<0.05), and Example 1 was relatively better than Comparative Example 1. There was an improvement in wrinkles around the eyes.

Experimental Example 9: Evaluation of tyrosinase enzyme activity inhibition

The whitening activity effect of the cosmetic of Example 1 was evaluated through the ability to inhibit tyrosinase enzyme activity. 1mL of 0.1M sodium phosphate buffer (pH 6.9), various concentrations of the cosmetic sample of Example 1, and 30μl of mushroom tyrosinase (1 KU/mL) were mixed and reacted at 37°C for 10 minutes, followed by 1.5mM L-tyrosine 30 as a substrate. ul was mixed and reacted at 25 ° C. for 2 minutes, and DOPA chrome generated in the reaction solution was measured at 475 nm, and the results are shown in Table 12. The absorbance of the control group was referred to as Ab _control , and the absorbance of the sample was referred to as Ab _sample , and was calculated according to Equation 3 below.

[Equation 3]

Tyrosinase enzyme activity inhibition rate (%) = (1-Ab _sample ) / Ab _control *100%

Classification (cosmetic sample concentration, sample)	Tyrosinase enzyme activity inhibition rate (%)
0.1 mg/ml	9.7%
0.3 mg/ml	20.1%
0.5 mg/ml	22.3%
0.7 mg/ml	30.5%
1.0 mg/ml	37.4%
1.5 mg/ml	38.2%

Through the evaluation of the tyrosinase enzyme activity inhibition rate in Table 12, it was confirmed that the cosmetic of the present invention has a skin whitening effect through inhibition of the tyrosinase enzyme activity.

Through the above examples and experimental examples, it was confirmed that the cosmetic of the present invention has various effects such as skin whitening and skin wrinkle improvement, and it was confirmed that the cosmetic of the present invention can provide cosmetics for various skin cosmetics. .

Simple modifications or changes of the present invention can be easily performed by those skilled in the art, and all such modifications or changes can be considered to be included in the scope of the present invention.

Claims (9)

1. A first step of preparing a first liquid by adding purified water and a viscosity modifier to the reactor and stirring for 40 to 80 minutes at 2500 to 3500 rpm;

   A second step of adding the second liquid to the reactor in which the first liquid was prepared and performing stirring at 2000 to 3000 rpm for 40 to 80 minutes;

   Step 3 of stirring for 40 to 60 minutes at 2000 to 3000 rpm after adding the third liquid to the reactor in which Step 2 was performed;

   Step 4 of performing stirring for 20 to 40 minutes at 1800 to 2800 rpm after adding a pH adjuster to the reactor in which Step 3 was performed; and

   Step 5 of adding the fourth liquid to the reactor in which step 4 was performed and stirring for 20 to 40 minutes at 1800 to 2800 rpm;

   Step 6 of filtering and defoaming the mixed solution after step 5; and

   A process including step 7 of aging the mixed solution after step 6 is performed,

   The first liquid contains a viscosity modifier and purified water,

   The second liquid is a conditioning agent containing at least one selected from glycereth-26, allantoin, hydrolyzed collagen, and adenosine skin; moisturizers including glycerin and butylene glycol; preservatives; and a crosslinking agent;

   The third liquid is a skin conditioning agent containing at least one selected from sunflower seed oil, dimethicone, cetylethylhexanoate and glycerylstearate; surfactants including PEG-100 stearate; humectants including Caprylic/Capric Triglyceride; An emulsifier containing at least one selected from polysorbate 60, stearic acid and sorbitan sesquioleate; And an emulsion stabilizer; including,

   The pH adjusting agent includes arginine,

   The fourth liquid is a functional extract containing a pearl extract and a natural plant complex extract; Lactobacillus fermentation lysate; collagen synthesis promoter; And fragrance; manufacturing method of skin elasticity improving cosmetics of the emulsion formulation utilizing the skin elasticity component material of the plant finger root of natural origin, characterized in that it comprises a.
2. The method of claim 1, wherein in the second step, 18 to 22 parts by weight of the second liquid is added to 100 parts by weight of the first liquid,

   In step 3, 8 to 12 parts by weight of the third solution is added to 100 parts by weight of the first solution,

   In step 5, 10 to 15 parts by weight of the 4th liquid is added to 100 parts by weight of the 1st liquid, Method for producing emulsion-type skin elasticity improving cosmetics using natural plant finger root skin elasticity component material .
3. Based on 100 parts by weight of the first solution, 18 to 22 parts by weight of the second solution, 8 to 12 parts by weight of the third solution, and 10 to 15 parts by weight of the fourth solution,

   The first liquid contains a viscosity modifier and purified water,

   The second liquid is a conditioning agent containing at least one selected from glycereth-26, allantoin, hydrolyzed collagen, and adenosine skin; moisturizers including glycerin and butylene glycol; preservatives including 1,2-hexanediol; And a crosslinking agent including sodium hyaluronate;

   The third liquid is a skin conditioning agent containing at least one selected from sunflower seed oil, dimethicone, cetylethylhexanoate and glycerylstearate; surfactants including PEG-100 stearate; humectants including Caprylic/Capric Triglyceride; An emulsifier containing at least one selected from polysorbate 60, stearic acid and sorbitan sesquioleate; And an emulsion stabilizer containing cetearyl alcohol;

   The fourth liquid is a functional extract containing a pearl extract and a natural plant complex extract; Lactobacillus fermentation lysate; collagen synthesis promoter; And fragrance; skin elasticity improvement cosmetic of the emulsion formulation utilizing the skin elasticity component material of the plant finger root of natural origin, characterized in that it comprises a.
4. The method of claim 3, wherein the first liquid contains 0.20 to 0.40% by weight of a viscosity modifier and the remaining amount of purified water,

   The second liquid comprises 18.5 to 21.0% by weight of the skin conditioning agent, 10 to 12.0% by weight of the crosslinking agent, 13.0 to 16.0% by weight of the whitening agent, 10 to 15.0% by weight of the preservative and the remaining amount of the moisturizing agent. Emulsion-type skin elasticity improvement cosmetic using the skin elasticity component of the natural plant finger root.
5. The method of claim 3, wherein the third liquid comprises 15.0 to 20.0% by weight of the emulsifier, 5.0 to 8.0% by weight of the emulsion stabilizer, 10.0 to 12.0% by weight of the surfactant, 5.0 to 8.0% by weight of the humectant, and the remaining amount of the skin. Contains a conditioning agent,

   The fourth liquid contains 5.0 to 8.0% by weight of lactobacillus fermented lysate, 1.0 to 2.5% by weight of collagen synthesis promoter, 0.1 to 0.8% by weight of fragrance, and the remaining amount of functional extract,

   The functional extract is a cosmetic for improving skin elasticity of an emulsion formulation using a skin elasticity component material of a natural plant finger root, characterized in that it comprises a pearl extract and a natural plant complex extract in a weight ratio of 1: 3.5 to 5.0.
6. The method of claim 5, wherein the natural plant complex extract is finger root rhizome extract, centella asiatica extract, vitamin tree extract, green tea extract, matricaria extract, low-danseon extract, mulberry leaf extract, hawthorn fruit extract, peppermint extract, hibiscus A cosmetic composition for improving skin elasticity in an emulsion formulation using a skin elasticity component material of a natural plant finger root, comprising a flower extract, a rosemary extract, a quince extract and a red chokeberry extract.
7. According to claim 6,

   The natural plant complex extract is based on 100 parts by weight of finger root rhizome extract, 15 to 30 parts by weight of Centella asiatica extract, 0.2 to 2.0 parts by weight of vitamin tree extract, 0.2 to 2.0 parts by weight of green tea extract, 0.2 to 2.0 parts by weight of Matricaria , 0.1 ~ 1.0 parts by weight of low tanning extract, 0.1 ~ 1.0 parts by weight of mulberry leaf extract, 0.1 ~ 1.0 parts by weight of hawthorn fruit extract, 0.1 ~ 1.0 parts by weight of peppermint extract, 0.1 ~ 1.0 parts by weight of hibiscus flower extract, 0.1 ~ 1.0 parts by weight of rosemary extract 1.0 parts by weight, 0.1 to 1.0 parts by weight of quince extract, and 0.1 to 1.0 parts by weight of red chokeberry extract, characterized by including 0.1 to 1.0 parts by weight of natural plant finger root skin elasticity improvement cosmetic of emulsion formulation using skin elasticity component material.
8. The method of claim 6, wherein the finger root rhizome extract is a finger root rhizome ethanol extract or a water fraction of the finger root rhizome ethanol extract. Elasticity improvement cosmetic.
9. Claims 3 to 8, characterized in that the cosmetic composition of any one item selected from the emulsion formulation improving skin elasticity cosmetic.

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