WO2024019212A1 - Cosmetic composition having whitening and antioxidant functions - Google Patents

Cosmetic composition having whitening and antioxidant functions Download PDF

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WO2024019212A1
WO2024019212A1 PCT/KR2022/012106 KR2022012106W WO2024019212A1 WO 2024019212 A1 WO2024019212 A1 WO 2024019212A1 KR 2022012106 W KR2022012106 W KR 2022012106W WO 2024019212 A1 WO2024019212 A1 WO 2024019212A1
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extract
parts
weight
natural
powder
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French (fr)
Korean (ko)
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조규태
조혜인
조혜진
전지수
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(주)바이오시엠
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin

Definitions

  • the present invention relates to a cosmetic composition having whitening and antioxidant functions.
  • the skin is composed of the epidermis, dermis, and subcutaneous tissue.
  • the most important role of the skin barrier is to keep our body, which is made up of about 80% water, safe from the external environment, and this barrier function is created and maintained in the epidermis, the outer layer of the skin.
  • the cells that make up the epidermis are composed of keratinocytes, Langerhans cells, Merkel cells, and melanocytes, which determine the color of the skin.
  • the dermis accounts for approximately 90% of the skin, is 10 to 40 times thicker than the epidermis, and is composed of collagen and elasticity-enhancing fibers that are involved in skin wrinkles.
  • melanocytes that produce melanin pigment There are many factors that determine human skin color, and the activity of melanocytes that produce melanin pigment, distribution of blood vessels, skin thickness, and other genetic factors play an important role. Among these, the activity of melanocytes in the human body to produce melanin pigment through the action of various enzymes such as tyrosinase is known to be the most important factor.
  • melanin is a dark brown pigment produced by oxidative polymerization of phenolic compounds. It not only determines human skin color, but also absorbs ultraviolet rays and exerts an antioxidant effect as a free radical scavenger to protect the skin. It plays an important role. However, if the skin is excessively exposed to ultraviolet rays, or melanin is overproduced due to factors such as hormonal changes or stress, it can cause pigmentation in the skin, making the skin dark and cloudy, or causing spots or freckles, which has a negative effect on skin beauty. You can.
  • whitening ingredients include substances that inhibit tyrosinase enzyme activity such as kojic acid, arbutin, alpha-bisabolol, ethyl ascobyl ether, and ascorbyl.
  • substances that inhibit the oxidation of tyrosine to melanin such as ascobyl glucoside and magnesium ascobyl phosphate.
  • melanin pigment By inhibiting the synthesis of melanin pigment, they can brighten skin tone and achieve skin whitening, or improve hyperpigmentation of the skin such as spots and freckles caused by ultraviolet rays, hormones, or genetic factors.
  • the most widely known whitening ingredient is ascorbic acid, which has far superior whitening and antioxidant effects compared to other whitening ingredients and is relatively economical in price. However, it has poor stability over time, so its whitening and antioxidant effects are significantly reduced when used for a long time. There is.
  • Patent Document 1 Republic of Korea Patent No. 10-2350285
  • the present invention was created to solve the above problems, and the purpose of the present invention is to solve the problem by applying a natural complex extract to maintain stability over time, which is a disadvantage of ascorbic acid, which has excellent whitening and antioxidant activity.
  • a natural complex extract to maintain stability over time, which is a disadvantage of ascorbic acid, which has excellent whitening and antioxidant activity.
  • four types of whitening and antioxidant ingredients were additionally added to further increase whitening and antioxidant activity.
  • a cosmetic composition with whitening and antioxidant functions containing glutathione, tocopherol, natural complex extract, ascorbic acid, ethyl ascorbyl ether, and tranexamic acid.
  • the natural complex extract is characterized in that it includes lotus seed extract, honeysuckle flower extract, burdock seed extract, Gimo root extract, Forsythia fruit extract, propolis extract, centella asiatica extract, and Gilge extract.
  • the first mixed powder a mixed solvent of acetone and chloroform mixed at a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMIM TFSI, 1-Ethyl-
  • EMIM TFSI 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide
  • the first natural product comprising lotus seed extract and burdock seed extract, comprising the steps of extracting a solution containing the active ingredient by mixing 3-methylimidazolium bis (trifluoromethylsulfylimide); and concentrating and freeze-drying the extracted solution under reduced pressure. Preparing an extract;
  • Preparing a second mixed powder containing honeysuckle flower powder, root root powder, and forsythia fruit powder by washing honeysuckle flowers, root roots, and forsythia fruit, cutting them into appropriate sizes, freeze-drying, and pulverizing them; mixing the second mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105°C for 10-14 hours; After performing the first hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering through filter paper, mixing the remaining solids with water again, and performing the second hot water extraction at a temperature of 135-145°C for 4-8 hours; After secondary hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a second extract; and mixing the first extract and the second extract; preparing a second natural extract comprising a honeysuckle flower extract, a root extract, and a forsythia fruit extract;
  • Preparing a third mixed powder containing propolis powder, centella asiatica powder, and apricot root by washing propolis, centella asiatica, and apricot root, cutting them into appropriate sizes, freeze-drying, and pulverizing them; extracting a solution containing the active ingredient by mixing the third mixed powder and ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure; preparing a third natural extract including propolis extract, Centella asiatica extract, and Gilge extract;
  • Preparing a natural complex extract by mixing 28-32 parts by weight of the first natural extract, 28-32 parts by weight of the second natural extract, and 38-42 parts by weight of the third natural extract;
  • Glutathione 0.3-0.7 parts by weight, tocopherol 0.5-2 parts by weight, 0.5-3 parts by weight of the above natural complex extract, ascorbic acid 9-20 parts by weight, ethyl ascorbyl ether 1-2 parts by weight, and tranexamic acid 1-3 It provides a method for producing a cosmetic composition having whitening and antioxidant functions, comprising the step of producing a composition containing parts by weight.
  • the cosmetic composition according to the present invention has antioxidant activity and whitening function.
  • Figure 1 shows a flow chart of a method for producing a first natural extract provided in one aspect according to the present invention
  • Figure 2 is a flowchart showing a method for producing a second natural extract provided in one aspect according to the present invention
  • Figure 3 is a flowchart showing a method for producing a third natural extract provided in one aspect according to the present invention.
  • Figure 4 shows the electron donating ability measurement test results of examples and comparative examples of skin formulations
  • Figure 5 shows the electron donating ability measurement test results of Examples and Comparative Examples of lotion formulations
  • Figure 6 shows the electron donating ability measurement test results of Examples and Comparative Examples of serum formulations
  • Figure 7 shows the electron donating ability measurement test results of Examples and Comparative Examples of cream formulations
  • Figure 8 shows the results of a test for measuring Tyrosinase inhibitory activity of examples and comparative examples of skin formulations
  • Figure 9 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of lotion formulations.
  • Figure 10 shows the results of a test for measuring Tyrosinase inhibitory activity of examples and comparative examples of serum formulations
  • Figure 11 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of cream formulations.
  • a cosmetic composition with whitening and antioxidant functions containing glutathione, tocopherol, natural complex extract, ascorbic acid, ethyl ascorbyl ether, and tranexamic acid.
  • Glutathione is a substance produced in the liver and is a type of protein that protects cells and tissues from active oxygen. It has an excellent antioxidant effect and suppresses melanin production in the skin, contributing to the whitening effect.
  • the glutathione preferably contains 0.3-1 part by weight, and more preferably 0.3-0.7 parts by weight. If used in a small amount within the above content range, there is a problem that there is almost no antioxidant activity and whitening effect, and if used in excessive amounts, there is a problem that side effects such as skin redness occur.
  • the tocopherol is a fat-soluble vitamin that can easily penetrate the double lipid membrane of cells, effectively exhibiting antioxidant activity and inhibiting melanin biosynthesis within melanocytes of epidermal tissue, and the excellent antioxidant effect of water-soluble ascorbic acid and tyrosinase. By maximizing the activity inhibition effect and melanin biosynthesis inhibition effect, it effectively prevents skin pigmentation and has excellent whitening effects such as improving skin color and skin gloss.
  • the tocopherol preferably contains 0.5-3 parts by weight, and more preferably 0.5-2 parts by weight. If used in a small amount within the above content range, there is a problem that the antioxidant activity is minimal, and if used in excessive amounts, there is a problem that the skin becomes dry or itchy side effects occur.
  • Ascorbic acid is also called vitamin C, and its effects can be divided into three categories: antioxidant, collagen synthesis, and whitening.
  • Ultraviolet rays have the greatest impact on the skin. The skin is always damaged by ultraviolet rays, and the free radicals generated by these ultraviolet rays are the main cause of skin aging.
  • Ascorbic acid is an antioxidant that inhibits the action of free radicals and causes aging. It shows a preventive effect. In addition, it has the effect of improving and preventing wrinkles by playing an auxiliary role in the synthesis of collagen, the main component of the dermis.
  • the ascorbic acid preferably contains 9-25 parts by weight, and more preferably contains 9-20 parts by weight.
  • the ascorbic acid preferably contains 9-25 parts by weight, and more preferably contains 9-20 parts by weight.
  • Ethyl Ascorbyl Ether is a substance or derivative synthesized from ascorbic acid and has excellent skin aging, wrinkle improvement, and whitening functions, so it is applied together with ascorbic acid to overcome the disadvantage of ascorbic acid's instability. It can show a high whitening function improvement effect. Moreover, when applied, it shows superior whitening function without skin irritation.
  • the ethyl ascorbyl ether preferably contains 0.5-4 parts by weight, and more preferably 1-2 parts by weight. If used less than the above content range, there is a problem of almost no antioxidant activity and whitening effect, and if used in excessive amounts, there is a problem that it is difficult to be absorbed into the skin, and when applied to sensitive skin, the safety for the skin is reduced.
  • the tranexamic acid is a synthetic amino acid and is a white crystal derived from the amino acid lysine. Originally, it is a substance used in the medical field due to its anti-inflammatory, anti-allergic and hemostatic effects. It is widely used for eczema, urticaria, tonsillitis, and hemostatic agents, and its safety has been confirmed. By applying this to cosmetic compositions, it can improve the skin whitening function as well as the function of repairing the skin barrier and restoring damaged skin.
  • the tranexamic acid preferably contains 1-4 parts by weight, and more preferably 1-3 parts by weight.
  • the natural complex extract includes lotus seed extract, honeysuckle flower extract, burdock seed extract, Gimo root extract, Forsythia fruit extract, propolis extract, centella asiatica extract, and Gilge extract.
  • the natural complex extract preferably contains 0.5-5 parts by weight, and more preferably contains 0.5-3 parts by weight.
  • the lotus seed (Nelumbo Nucifera Seed) refers to the seed of the lotus flower.
  • the lotus is a perennial aquatic plant of the order Ranunculaceae and the water lily family.
  • the flowers bloom in July-August, are red or white, have one flower at the end of the flower stem, and have seeds in the holes of the flower stalk. Its safety has been proven as various parts of lotus have been used as food or herbal medicine for a long time.
  • lotus seed extract contains asparagine and arginine, which can remove free radicals that are fatal to skin aging and help make dull skin tone clear and bright. It can also help soothe the skin and reduce oxidative stress on the skin by suppressing skin peroxidation.
  • honeysuckle flower extract contains ingredients such as ginol, sterols, glycosides, chlorogenic acid, flavonoid compounds, tannin, isochlorogenic acid, caffeic acid, and quercetin. It maximizes whitening function by assisting with whitening ingredients such as big acid.
  • burdock seeds refer to the seeds of burdock.
  • Burdock is 50-150 cm tall.
  • the taproot grows 30-60 cm long and a stem emerges from the end.
  • the leaves attached to the roots come out in clusters and have long petioles.
  • the stems are alternate and heart-shaped.
  • the outside is dark green, but the back has dense white fluff, and there are teeth shaped like this on the edges.
  • the flowers bloom in July-August and are black-purple, and the flower heads hang in corymbs at the ends of branches.
  • the involucre is round, the bracts are needle-shaped, and the ends look like hooks.
  • the flowers are tubular, the seeds are black, and the tubular hairs are brown.
  • the fruit is an achene and ripens in September. It is hardy, rarely suffers from diseases, is very resistant to cold, and is not particularly sensitive to soil type. Propagation is done by seed or by dividing. It is a naturalized plant native to Europe. Varieties include Noyacheon, which has long and thick roots, and Sacheon, which has good flesh and short roots. The root contains inulin and some palmitic acid. In Europe, it is used as a diuretic and diaphoretic, and the seeds are used as a diuretic when there is swelling, and as an antidote to sore throats and poisonous insects.
  • Burdock seeds are also called Ubangja, and are also used as a medicinal herb that has the effect of dispersing wind heat by dissipating it, clearing waste, causing acne to grow, reducing swelling, and detoxifying.
  • burdock seed extract When used as a cosmetic ingredient, burdock seed extract contains arctigenin, vitamin A, vitamin B1, etc., which inhibits melanin production and has a whitening effect.
  • the Anemarrhena Asphodeloides Root refers to the root of the Anemarrhena Asphodeloides Root.
  • Jimo is a perennial herb belonging to the Liliaceae family. Its roots contain a large amount of saponin, the same as the main ingredient of ginseng, and are used for medicinal purposes. It helps improve cough, phlegm, and thirst caused by fever.
  • this zygote root extract contains borphyrin, saponin, etc., which helps improve skin tone, soothe the skin, enhances moisturizing, releases toxins, and has antioxidant effects.
  • the Forsythia Suspensa Fruit refers to the fruit of Forsythia Suspensa.
  • Forsythia is also called Yeongyo. It is a medicinal product of the fruit of Uiseong Forsythia, a member of the ash tree family. It has a unique smell, tastes bitter, and is slightly cold in nature. In addition, it reduces fever and detoxifies, so it reduces heart heat in the early stage of heat illness, is used for high fever and mental confusion, and is used for boils, rashes, appendicitis, pulmonary rust, lymphadenitis, and sore throat, and has a diuretic and anti-inflammatory effect.
  • forsythia fruit extract contains ingredients such as saponin, oleanolic acid, arctigenin, and matairesinol glucoside, which helps improve moisturizing power to keep the skin moist, and protects the skin from harmful substances with its antioxidant effect. protect
  • Propolis is a dark gray-brown powder that has no unpleasant odor and has a unique flavor. Bees mix their own saliva and enzymes with substances such as resins extracted from various plants for their own survival and reproduction. As a made substance, the most common ingredients are organic substances and minerals (inorganic salts). Minerals, vitamins, amino acids, fats, organic acids, flavonoids, etc. play an important role in cell metabolism, and terpenes have anti-cancer properties. Its main effects include anti-inflammatory, antioxidant, immune enhancement, and moisturizing properties. When used as a cosmetic ingredient, this propolis extract contains over 20 different types of flavonoids, maximizing its antioxidant role in protecting the skin from free radicals.
  • Platycodon Grandiflorus also called Gilgyeonggeun
  • Gilgyeong is a medicinal herb made by removing the roots or pericarp of campanula and bellflower root, and is known to have a slight odor, bitter taste, and flat properties.
  • Gilgyeong acts on the lungs to treat symptoms such as excessive seawater and phlegm and difficulty breathing, and has the effect of stopping coughing and removing phlegm. It is also used when there is general edema and small amount of urine due to difficulty in urinating. Therefore, it is prescribed and used for sore throat, cough due to cold, phlegm, nasal congestion, asthma, bronchitis, pleurisy, headache, chills, tonsillitis, etc.
  • this Gilge extract contains ingredients such as 3''-O-acetylplaticonic acid A, 3''-O-acetylpolygalaxin D, and polygalaxin D3, thereby suppressing oxidation of cosmetics. It prevents deterioration, decay, and deterioration of cosmetics, and hinders the activity of external substances harmful to the skin.
  • centella asiatica is a slender perennial vine that grows naturally in tropical regions. It is also called tiger grass because it is said that tigers used to roll in this grass field when they had injuries on their bodies since ancient times.
  • the leaves and stems contain active ingredients such as asiaticoside, saponin, and matecasque acid, which helps in maintaining smooth skin. gives.
  • this Centella asiatica extract soothes irritated skin by suppressing melanin and prevents pigmentation to assist with whitening function.
  • the cosmetic composition having whitening and antioxidant functions according to the present invention contains 1-6 parts by weight of butylene glycol, 1-3 parts by weight of tromethamine, 1-6 parts by weight of propanediol, and ammonium acryloyldimethyl tau. It is preferable to include 0.1-1 part by weight of rate/VP copolymer, 1-3 parts by weight of 1,2-hexanediol, and the remainder containing water, and 1-6 parts by weight of dipropylene glycol and butylene glycol.
  • ⁇ parts 1-3 parts by weight of 1,2-hexanediol, 0.01-0.1 parts by weight of ethylhexylglycerin, 1-6 parts by weight of dipropylene glycol, 1-5 parts by weight of Laureth-23, sodium hyaluronate It is preferable to include 0.1-1 part by weight and the remainder of water, 1-6 parts by weight of dipropylene glycol, 1-6 parts by weight of butylene glycol, 1-6 parts by weight of propanediol, and tromethamine.
  • xanthan gum 0.1-0.5 parts by weight, ammonium acryloyldimethyl taurate/VPicopolymer 0.1-1 parts by weight, polysorbate 80 0.1-2 parts by weight, cetearyl alcohol 1-5 parts by weight parts, dicaprylyl carbonate 1-8 parts by weight, dimethicone, dimethicone/vinyl dimethicone crosspolymer 0.1-2 parts by weight, 1,2-hexanediol 1-3 parts by weight, ethylhexylglycerin 0.01 It is preferable to include water at -0.1 part by weight and the remaining balance.
  • the first mixed powder a mixed solvent of acetone and chloroform mixed at a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMIM TFSI, 1-Ethyl-
  • EMIM TFSI 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide
  • the first natural product comprising lotus seed extract and burdock seed extract, comprising the steps of extracting a solution containing the active ingredient by mixing 3-methylimidazolium bis (trifluoromethylsulfylimide); and concentrating and freeze-drying the extracted solution under reduced pressure. Preparing an extract;
  • Preparing a second mixed powder containing honeysuckle flower powder, root root powder, and forsythia fruit powder by washing honeysuckle flowers, root roots, and forsythia fruit, cutting them into appropriate sizes, freeze-drying, and pulverizing them; mixing the second mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105°C for 10-14 hours; After performing the first hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering through filter paper, mixing the remaining solids with water again, and performing the second hot water extraction at a temperature of 135-145°C for 4-8 hours; After secondary hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a second extract; and mixing the first extract and the second extract; preparing a second natural extract comprising a honeysuckle flower extract, a root extract, and a forsythia fruit extract;
  • Preparing a third mixed powder containing propolis powder, centella asiatica powder, and apricot root by washing propolis, centella asiatica, and apricot root, cutting them into appropriate sizes, freeze-drying, and pulverizing them; extracting a solution containing the active ingredient by mixing the third mixed powder and ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure; preparing a third natural extract including propolis extract, Centella asiatica extract, and Gilge extract;
  • Preparing a natural complex extract by mixing 28-32 parts by weight of the first natural extract, 28-32 parts by weight of the second natural extract, and 38-42 parts by weight of the third natural extract;
  • Glutathione 0.3-0.7 parts by weight, tocopherol 0.5-2 parts by weight, 0.5-3 parts by weight of the above natural complex extract, ascorbic acid 9-20 parts by weight, ethyl ascorbyl ether 1-2 parts by weight, and tranexamic acid 1-3 It provides a method for producing a cosmetic composition having whitening and antioxidant functions, comprising the step of producing a composition containing parts by weight.
  • Figures 1 to 3 show a flow chart of the method for producing the natural extract provided in one aspect according to the present invention.
  • the cosmetic composition having whitening and antioxidant functions according to the present invention The manufacturing method is explained in detail at each step.
  • the method for producing a cosmetic composition with whitening and antioxidant functions includes preparing a first natural extract containing lotus seed extract and burdock seed extract.
  • the step of preparing the first natural extract includes washing lotus seeds and burdock seeds, cutting them into appropriate sizes, freeze-drying, and pulverizing them to prepare a first mixed powder containing lotus seed powder and burdock seed powder;
  • the first mixed powder a mixed solvent of acetone and chloroform mixed at a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMIM TFSI, 1-Ethyl- It includes extracting a solution containing the active ingredient by mixing 3-methylimidazolium bis (trifluoromethylsulfylimide), and concentrating and freeze-drying the extracted solution under reduced pressure.
  • EMIM TFSI 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide
  • lotus seed extract and burdock seed extract are prepared, and the extract is prepared using lotus seed and burdock seed together.
  • the content of active ingredients polyphenols, flavonoids, etc.
  • Extraction is performed by introducing ionic liquid as a solvent and additive.
  • the lotus seeds and burdock seeds are washed, cut into appropriate sizes, freeze-dried, and pulverized to prepare a first mixed powder containing lotus seed powder and burdock seed powder.
  • the first mixed powder a mixed solvent of acetone and chloroform mixed at a volume ratio of 1:1
  • EMIM TFSI 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide
  • a solution containing the active ingredient is extracted by mixing Ethyl-3-methylimidazolium bis (trifluoromethylsulfylimide).
  • the first mixed powder, mixed solvent, and EMIM TFSI are preferably mixed at a mixing ratio of 1:20:0.5 (first mixed powder (g): mixed solvent (ml): EMIm TFSI (g)).
  • the extraction is preferably performed at a temperature range of 20-40°C for 12-24 hours, and more preferably at a temperature of 30°C for 12 hours. Additionally, the extraction is preferably performed using an ultrasonic process.
  • the extracted solution is concentrated under reduced pressure and freeze-dried. Specifically, the extract is filtered through filter paper, then concentrated under reduced pressure at 55°C in a concentrator and freeze-dried.
  • the method for producing a cosmetic composition with whitening and antioxidant functions includes the step of preparing a second natural extract containing honeysuckle flower extract, syringa root extract, and forsythia fruit extract.
  • the step of preparing the second natural extract includes washing honeysuckle flowers, roots and forsythia fruits, cutting them into appropriate sizes, freeze-drying and pulverizing them to produce honeysuckle flower powder, root powder and forsythia fruit powder.
  • Preparing a second mixed powder mixing the second mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105°C for 10-14 hours; After performing the first hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering through filter paper, mixing the remaining solids with water again, and performing the second hot water extraction at a temperature of 135-145°C for 4-8 hours; After secondary hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a second extract; and mixing the first extract and the second extract.
  • honeysuckle flower extract, root extract, and forsythia fruit extract are prepared, and the extract is prepared using honeysuckle flower, root root, and forsythia fruit together.
  • extracts are extracted from vines, roots, or fruits.
  • active ingredients polyphenols, flavonoids, etc.
  • a hot water extraction process is introduced, and in particular, a multi-stage hot water extraction process is introduced to extract them.
  • the second mixed powder and water are mixed, stirred, and first hot water extraction is performed at a temperature of 95-105°C for 10-14 hours.
  • the first hot water extraction is preferably performed at a temperature of 98-102°C for 11-13 hours, and most preferably at a temperature of 100°C for 12 hours.
  • the first hot water extraction can be performed at a temperature of 100°C for 12 hours to maximize extraction performance during the second hot water extraction, thereby producing an extract with a high content of active ingredients.
  • the second hot water extraction is performed at a temperature of 135-145°C for 4-8 hours.
  • the secondary hot water extraction is preferably performed at a temperature of 138-142°C for 5-7 hours, and most preferably at a temperature of 140°C for 6 hours.
  • the secondary hot water extraction is performed at a high temperature of 140°C for 6 hours, allowing the active ingredient to be extracted at a higher content from the solid obtained after the first hot water extraction.
  • the method for producing a cosmetic composition with whitening and antioxidant functions includes the step of preparing a third natural extract including propolis extract, centella asiatica extract, and Gilge extract.
  • the step of preparing the third natural extract includes washing propolis, centella asiatica, and apricot chinensis, cutting them into appropriate sizes, freeze-drying, and pulverizing them to prepare a third mixed powder containing propolis powder, centella asiatica powder, and apricot chinensis powder. step; extracting a solution containing the active ingredient by mixing the third mixed powder and ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure.
  • propolis extract, centella asiatica extract, and Gilgeum extract are prepared, and the extract is prepared using propolis, Centella asiatica, and Gilgeria together, and the extract is prepared by using propolis, Centella asiatica, and Gilgeria together, so that the propolis extract becomes water.
  • Centella asiatica extract and Gilge extract act as natural surfactants to increase the solubility of propolis extract, allowing it to be easily mixed into cosmetic compositions.
  • the third mixed powder and ethanol are mixed to extract a solution containing the active ingredient.
  • the extraction is preferably performed at a temperature range of 20-40°C for 12-24 hours, and more preferably at a temperature of 20°C for 12 hours. Additionally, the extraction is preferably performed by a dipping process.
  • the extracted solution is concentrated under reduced pressure and freeze-dried. Specifically, the extract is filtered through filter paper, then concentrated under reduced pressure at 55°C in a concentrator and freeze-dried.
  • the method for producing a cosmetic composition having whitening and antioxidant functions according to the present invention is to use 28-32 parts by weight of the first natural extract, 28-32 parts by weight of the second natural extract, and 38-42 parts by weight of the third natural extract. It includes the step of mixing the parts to produce a natural complex extract.
  • the first natural extract, the second natural extract, and the third natural extract prepared in the previous step are all mixed, and 28-32 parts by weight of the first natural extract, 28-32 parts by weight of the second natural extract, and the third natural extract are mixed together.
  • 3 Prepare a natural complex extract by mixing 38-42 parts by weight of natural extract.
  • a natural complex extract is prepared by mixing 29-31 parts by weight of the first natural extract, 29-31 parts by weight of the second natural extract, and 39-41 parts by weight of the third natural extract, and most preferably, , 30 parts by weight of the first natural extract, 30 parts by weight of the second natural extract, and 40 parts by weight of the third natural extract are mixed to prepare a natural complex extract.
  • a natural complex extract obtained by mixing the first to third natural extracts in the above mixing ratio can be applied as a cosmetic composition to exhibit excellent antioxidant activity and whitening function.
  • the method for producing a cosmetic composition with whitening and antioxidant functions according to the present invention is 0.3-0.7 parts by weight of glutathione, 0.5-2 parts by weight of tocopherol, 0.5-3 parts by weight of the natural complex extract, and 9-20 parts by weight of ascorbic acid. parts, 1-2 parts by weight of ethyl ascorbyl ether, and 1-3 parts by weight of tranexamic acid.
  • Glutathione 0.3-0.7 parts by weight, tocopherol 0.5-2 parts by weight, 0.5-3 parts by weight of the above natural complex extract, ascorbic acid 9-20 parts by weight, ethyl ascorbyl ether 1-2 parts by weight, and tranexamic acid 1-3 It is more preferred to prepare a composition comprising parts by weight.
  • 1-6 parts by weight of butylene glycol 1-3 parts by weight of tromethamine, 1-6 parts by weight of propanediol, 0.1-1 parts by weight of ammonium acryloyl dimethyl taurate/VP copolymer, 1, It is preferable to include 1-3 parts by weight of 2-hexanediol and the remainder containing water, 1-6 parts by weight of dipropylene glycol, 1-6 parts by weight of butylene glycol, and 1-6 parts by weight of propanediol.
  • the remaining balance preferably contains water, 1-6 parts by weight of butylene glycol, 1-6 parts by weight of propanediol, 1-5 parts by weight of sodium lactate, and 1-3 parts by weight of 1,2-hexanediol.
  • a natural complex extract was prepared by mixing 30 parts by weight of the first natural extract prepared in Preparation Example 1, 30 parts by weight of the second natural extract prepared in Preparation Example 2, and 40 parts by weight of the third natural extract prepared in Preparation Example 3. Manufactured.
  • the skin formulation is manufactured by dissolving the aqueous part glutathione, ascorbic acid, ethyl ascorbyl ether, tranexamic acid, butylene glycol, tromethamine, and propanediol and the solubilized part tocopherol and laureth-23 at a temperature of 50°C. Then mix the aqueous part and the solubilized part. Add the thickener part by dissolving ammonium acryloyldimethyl taurate/VP copolymer in purified water heated to a temperature of 75-80°C, then add the additive parts, natural complex extract and 1,2-hexanediol. Manufactured. The content of each component is shown in Table 1 below.
  • the aqueous parts such as glutathione, ascorbic acid, ethyl ascorbyl ether, tranexamic acid, dipropylene glycol, butylene glycol, propanediol, tromethamine and the oil parts such as tocopherol and polysorbate are used.
  • 80 measure dicaprylyl carbonate, dimethicone, and dimethicone/vinyl dimethicone crosspolymer separately, heat to a temperature of 75-80°C, mix and stir the aqueous phase and oil phase (75-80°C) , Homo mix 3000 rpm, 5 minutes).
  • the thickener parts, xanthan gum and ammonium acryloyl dimethyl taurate/VP copolymer are dissolved in an azimixer at a temperature of 75-80°C, then added and mixed and stirred (75-80°C, Homo mix 3000 rpm, 5 minutes). do.
  • After cooling to a temperature of 50-60°C add the additive part natural complex extract, 1,2-hexanediol, and ethylhexylglycerin, mix and stir (50-60°C, Homo mix 2000 rpm, 5 minutes), and cool (30-35°C). °C) degassing.
  • Table 2 The content of each component is shown in Table 2 below.
  • the water part glutathione, ascorbic acid, ethyl ascorbyl ether, tranexamic acid, dipropylene glycol, butylene glycol, propanediol, tromethamine, the oil part tocopherol, polysorbate 80, Cetearyl alcohol, dicaprylyl carbonate, dimethicone, dimethicone/vinyl dimethicone crosspolymer were weighed separately, heated to a temperature of 75-80°C, mixed and stirred (75°C). -80°C, Homo mix 3000 rpm, 5 minutes).
  • the thickener part, xanthan gum, ammonium acryloyl dimethyl taurate/VP copolymer, is dissolved in an Azi mixer at a temperature of 75-80°C, then added and mixed and stirred (75-80°C, Homo mix 3000 rpm, 5 minutes). After cooling to a temperature of 50-60°C, add all the additive parts, natural complex extract, 1,2-hexanediol, and ethylhexylglycerin, mix and stir (50-60°C, Homo mix 2000 rpm, 5 minutes), and cool (30-60°C). 35°C) Degassing. The content of each component is shown in Table 4 below.
  • DPPH (2,2-diphenyl-1-picrylhydrazyl) assay is a quick and simple measurement method that is widely used to measure the antioxidant capacity of plants, food extracts, or single compounds.
  • DPPH is an organic nitrogen radical that is dark purple in color. When it comes into contact with a substance with antioxidant activity, it is reduced by accepting electrons from the hydrogen radical at a very rapid rate and is irreversibly converted to the stable compound 2,2-diphenyl-1-picryl hydrazine, forming a dark substance. It has the characteristic of fading purple color. The degree to which the color becomes lighter means that the DPPH radical scavenging ability of the extract or compound is high.
  • the DPPH assay measurement result is expressed as a percentage using the difference between the absorbance of the sample added group and the absorbance of the untreated group and can be obtained using the formula below.
  • A is the absorbance of the sample addition section
  • B is the absorbance of the untreated section.
  • the DPPH (2,2-diphenyl-1-picryl hydrazyl) solution used in the electron donating ability experiment was dissolved in methanol to make 0.25mM. After adding 90uL 0.25mM DPPH (1,1-diphenyl-2-picryl-hydrazyl) solution and 100uL MeOH to a 96 well plate, 10uL of each concentration of extract was added and reacted for 30 minutes under light blocking conditions. After reaction, the absorbance was measured at 517 nm using an absorbance meter. Ascorbic acid, Kojic acid, etc. are used as positive controls.
  • pigmentation is caused by various external stimuli such as ultraviolet rays and inflammation. This occurs in melanocytes present in the basal layer of the epidermis, and the mechanism of pigmentation caused by ultraviolet rays is mainly played by the tyrosinase expression control factor.
  • Tyrosinase inhibitory activity measurement experiment uses the fact that tyrosinase has DOPA oxidase activity, which oxidizes DOPA to DOPA quinone during the pigmentation process.
  • the enzyme activity inhibition ability of the sample is measured by comparing the amount of DOPA chrome produced by the reaction between tyrosinase and L-DOPA.
  • Sodium phosphate buffer for the tyrosinase inhibitory activity test was prepared and used at 0.175M, pH 6.8.
  • L-DOPA (3,4-dihydroxy-L-phenyl-alanine) and Tyrosinase from mushroom were dissolved in sodium phosphate buffer to 10mM and 1000U/mL, respectively.
  • the stability evaluation and confirmation experiment of cosmetic contents evaluates changes in appearance, odor, viscosity, pH, etc. when stored for a long period of time at three temperatures of 4°C, 40°C, and 25°C, and is conducted over a period of 1 day, 7 days, 3 weeks, 5 weeks, and 8 weeks. Conduct an experiment to check the appearance (color change) and odor.
  • Figure 4 shows the electron donating ability measurement test results of Examples and Comparative Examples of skin formulations
  • Figure 5 shows the electron donating ability measurement test results of Examples and Comparative Examples of lotion formulations
  • Figure 6 shows the electron donating ability measurement test results of Examples and Comparative Examples of serum formulations. These are the test results
  • Figure 7 shows the electron donating ability measurement test results of the cream formulation examples and comparative examples.
  • Figure 8 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of skin formulations
  • Figure 9 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of lotion formulations
  • Figure 10 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of serum formulations. These are the results of the inhibitory activity measurement test
  • Figure 11 shows the results of the Tyrosinase inhibitory activity measurement test of the examples and comparative examples of cream formulations.

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Abstract

The present invention provides a cosmetic composition having whitening and antioxidant functions, comprising glutathione, tocopherol, a natural complex extract, ascorbic acid, ethyl ascorbyl ether and tranexamic acid.

Description

미백 및 항산화기능을 가지는 화장품 조성물Cosmetic composition with whitening and antioxidant properties
본 발명은 미백 및 항산화기능을 가지는 화장품 조성물에 관한 것이다.The present invention relates to a cosmetic composition having whitening and antioxidant functions.
피부는 표피, 진피, 피하조직으로 구성되어 있다. 피부 장벽의 가장 중요한 역할은 약 80%의 물로 구성된 우리의 몸을 외부환경으로부터 안전하게 지켜주는 것이며, 이러한 장벽 기능은 피부 바깥층인 표피에서 생성, 유지되고 있다.The skin is composed of the epidermis, dermis, and subcutaneous tissue. The most important role of the skin barrier is to keep our body, which is made up of about 80% water, safe from the external environment, and this barrier function is created and maintained in the epidermis, the outer layer of the skin.
표피를 구성하는 세포들은 각질형성세포, 랑게르한스 세포, 머켈세포와 더불어 피부의 색을 결정하는 멜라닌 세포로 구성되어 있다. 진피는 피부의 약 90%를 차지하며, 두께가 표피의 10~40배에 달하며, 피부의 주름에 관여하는 콜라겐 및 탄력 증진섬유로 구성되어 있다.The cells that make up the epidermis are composed of keratinocytes, Langerhans cells, Merkel cells, and melanocytes, which determine the color of the skin. The dermis accounts for approximately 90% of the skin, is 10 to 40 times thicker than the epidermis, and is composed of collagen and elasticity-enhancing fibers that are involved in skin wrinkles.
인간의 피부색을 결정하는 요인에는 여러가지가 존재하는데, 멜라닌 색소를 생성하는 멜라노사이트(melanocyte)의 활성, 혈관의 분포와 피부의 두께 기타 유전적인 요인들이 중요하게 작용한다. 이 중 특히 티로시나제 등 여러 효소의 작용에 의해 인체 내 멜라노사이트가 멜라닌 색소를 생성하는 활성이 특히 가장 중요한 요인으로 알려져 있다. There are many factors that determine human skin color, and the activity of melanocytes that produce melanin pigment, distribution of blood vessels, skin thickness, and other genetic factors play an important role. Among these, the activity of melanocytes in the human body to produce melanin pigment through the action of various enzymes such as tyrosinase is known to be the most important factor.
한편, 멜라닌(melanin)은 페놀 화합물의 산화중합에 의하여 생성되는 흑갈색의 색소로, 인간의 피부색을 결정할 뿐 아니라 자외선을 흡수하거나 Free Radical Scavenger로서 항산화 효과를 나타내어 피부의 보호 작용을 하는 등 피부 내에서 중요한 역할을 담당하고 있다. 그러나, 피부가 자외선에 과도하게 노출되거나, 호르몬 변화나 스트레스 등 요인으로 인해 멜라닌이 과잉 생산될 경우, 피부에 색소 침착을 가져와 피부색이 어둡고 탁해지거나 기미나 주근깨 등을 유발하여 피부 미용에는 부정적으로 작용할 수 있다. Meanwhile, melanin is a dark brown pigment produced by oxidative polymerization of phenolic compounds. It not only determines human skin color, but also absorbs ultraviolet rays and exerts an antioxidant effect as a free radical scavenger to protect the skin. It plays an important role. However, if the skin is excessively exposed to ultraviolet rays, or melanin is overproduced due to factors such as hormonal changes or stress, it can cause pigmentation in the skin, making the skin dark and cloudy, or causing spots or freckles, which has a negative effect on skin beauty. You can.
일반적으로 알려진 미백　성분으로, 코지산(kojic acid) 또는 알부틴 (arbutin) 알파-비사보롤(alpha-bisabolol)등과 같은 티로시나제 효소활성을 억제하는 물질, 에칠아스코빌에텔(ethyl ascobyl ether), 아스코빌글루코사이드(ascobyl glucoside), 마그네슘아스코빌포스페이트(magnesium ascobyl phosphate) 등과 같은 티로신의 멜라닌으로 산화 억제하는 물질이 있다. 이들은 멜라닌 색소의 합성을 저해함으로써 피부 톤을 밝게 하고 피부　미백을 달성하거나, 자외선, 호르몬 또는 유전적 요소에 기인한 기미나 주근깨 등 피부의 과색소 침착증을 개선할 수 있다. 현재 가장 많이 알려져 있는 미백성분은 아스코빅애씨드로 미백 및 항산화 효과가 다른 미백성분에 비해 월등히 뛰어나고 가격적인 면에서도 비교적 경제적인 반면에 시간 경과에 따른 안정도가 떨어져서 장기간 사용시 미백 및 항산화 효과가 현격히 떨어지는 단점이 있다.Commonly known whitening ingredients include substances that inhibit tyrosinase enzyme activity such as kojic acid, arbutin, alpha-bisabolol, ethyl ascobyl ether, and ascorbyl. There are substances that inhibit the oxidation of tyrosine to melanin, such as ascobyl glucoside and magnesium ascobyl phosphate. By inhibiting the synthesis of melanin pigment, they can brighten skin tone and achieve skin whitening, or improve hyperpigmentation of the skin such as spots and freckles caused by ultraviolet rays, hormones, or genetic factors. Currently, the most widely known whitening ingredient is ascorbic acid, which has far superior whitening and antioxidant effects compared to other whitening ingredients and is relatively economical in price. However, it has poor stability over time, so its whitening and antioxidant effects are significantly reduced when used for a long time. There is.
(선행기술문헌)(Prior art literature)
(특허문헌) 특허문헌 1: 대한민국등록특허 제10-2350285호(Patent Document) Patent Document 1: Republic of Korea Patent No. 10-2350285
본 발명은 상기와 같은 문제점을 해결하기 위해 안출된 것으로서, 본 발명의 목적은 미백 및 항산화 활성이 뛰어난 아스코빅애씨드의 단점인 시간 경과에 따른 안정도를 유지하기 위해 천연복합추출물을 적용해서 문제를 해결하였으며, 이와 더불어 미백 및 항산화 활성을 더욱 높이기 위해 4종의 미백 및 항산화 성분을 추가로 함유시켰다.The present invention was created to solve the above problems, and the purpose of the present invention is to solve the problem by applying a natural complex extract to maintain stability over time, which is a disadvantage of ascorbic acid, which has excellent whitening and antioxidant activity. In addition, four types of whitening and antioxidant ingredients were additionally added to further increase whitening and antioxidant activity.
본 발명의 과제는 이상에서 언급한 과제들로 제한되지 않으며, 언급되지 않은 또 다른 과제들은 아래의 기재로부터 당업자에게 명확하게 이해될 수 있을 것이다.The object of the present invention is not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below.
상기 목적을 달성하기 위하여 본 발명은In order to achieve the above object, the present invention
글루타치온, 토코페롤, 천연복합추출물, 아스코빅애씨드, 에칠아스코빌에텔 및 트라넥사믹애씨드를 포함하는 미백 및 항산화기능을 가지는 화장품 조성물을 제공한다.Provides a cosmetic composition with whitening and antioxidant functions containing glutathione, tocopherol, natural complex extract, ascorbic acid, ethyl ascorbyl ether, and tranexamic acid.
또한, 상기 천연복합추출물은 연꽃씨추출물, 인동덩굴꽃추출물, 우엉씨추출물, 지모뿌리추출물, 당개나리열매추출물, 프로폴리스추출물, 병풀추출물 및 길경추출물을 포함하는 것을 특징으로 한다.In addition, the natural complex extract is characterized in that it includes lotus seed extract, honeysuckle flower extract, burdock seed extract, Gimo root extract, Forsythia fruit extract, propolis extract, centella asiatica extract, and Gilge extract.
나아가, 본 발명은Furthermore, the present invention
연꽃씨 및 우엉씨를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 연꽃씨분말 및 우엉씨분말을 포함하는 제1 혼합분말을 준비하는 단계; 상기 제1 혼합분말, 1:1의 부피비로 혼합된 아세톤 및 클로로포름의 혼합 용매 및 1-에틸-3-메틸이미다졸리움 비스(트리플루오로메틸설포닐)이미드(EMIM TFSI, 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfylimide)를 혼합하여 유효성분을 포함하는 용액을 추출하는 단계; 및 추출된 용액을 감압농축 및 동결건조하는 단계;를 포함하는 연꽃씨추출물 및 우엉씨추출물을 포함하는 제1 천연추출물을 제조하는 단계;Washing lotus seeds and burdock seeds, cutting them into appropriate sizes, freeze-drying, and pulverizing them to prepare a first mixed powder containing lotus seed powder and burdock seed powder; The first mixed powder, a mixed solvent of acetone and chloroform mixed at a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMIM TFSI, 1-Ethyl- The first natural product comprising lotus seed extract and burdock seed extract, comprising the steps of extracting a solution containing the active ingredient by mixing 3-methylimidazolium bis (trifluoromethylsulfylimide); and concentrating and freeze-drying the extracted solution under reduced pressure. Preparing an extract;
인동덩굴꽃, 지모뿌리 및 당개나리열매를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 인동덩굴꽃분말, 지모뿌리분말 및 당개나리열매분말을 포함하는 제2 혼합분말을 준비하는 단계; 상기 제2 혼합분말 및 물을 혼합하고, 교반을 수행하며 95-105℃의 온도에서 10-14시간 동안 1차 열수추출을 수행하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 135-145℃의 온도에서 4-8시간 동안 2차 열수추출을 수행하는 단계; 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조하는 단계; 및 상기 제1 추출물 및 제2 추출물을 혼합하는 단계;를 포함하는 인동덩굴꽃추출물, 지모뿌리추출물 및 당개나리열매추출물을 포함하는 제2 천연추출물을 제조하는 단계;Preparing a second mixed powder containing honeysuckle flower powder, root root powder, and forsythia fruit powder by washing honeysuckle flowers, root roots, and forsythia fruit, cutting them into appropriate sizes, freeze-drying, and pulverizing them; mixing the second mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105°C for 10-14 hours; After performing the first hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering through filter paper, mixing the remaining solids with water again, and performing the second hot water extraction at a temperature of 135-145°C for 4-8 hours; After secondary hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a second extract; and mixing the first extract and the second extract; preparing a second natural extract comprising a honeysuckle flower extract, a root extract, and a forsythia fruit extract;
프로폴리스, 병풀 및 길경을 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 프로폴리스분말, 병풀분말 및 길경분말을 포함하는 제3 혼합분말을 준비하는 단계; 상기 제3 혼합분말 및 에탄올을 혼합하여 유효성분을 포함하는 용액을 추출하는 단계; 및 추출된 용액을 감압농축 및 동결건조하는 단계;를 포함하는 프로폴리스추출물, 병풀추출물 및 길경추출물을 포함하는 제3 천연추출물을 제조하는 단계;Preparing a third mixed powder containing propolis powder, centella asiatica powder, and apricot root by washing propolis, centella asiatica, and apricot root, cutting them into appropriate sizes, freeze-drying, and pulverizing them; extracting a solution containing the active ingredient by mixing the third mixed powder and ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure; preparing a third natural extract including propolis extract, Centella asiatica extract, and Gilge extract;
상기 제1 천연추출물 28-32 중량부, 상기 제2 천연추출물 28-32 중량부 및 상기 제3 천연추출물 38-42 중량부를 혼합하여 천연복합추출물을 제조하는 단계; 및Preparing a natural complex extract by mixing 28-32 parts by weight of the first natural extract, 28-32 parts by weight of the second natural extract, and 38-42 parts by weight of the third natural extract; and
글루타치온 0.3-0.7 중량부, 토코페롤 0.5-2 중량부, 상기 천연복합추출물 0.5-3 중량부, 아스코빅애씨드 9-20 중량부, 에칠아스코빌에텔 1-2 중량부 및 트라넥사믹애씨드 1-3 중량부를 포함하는 조성물을 제조하는 단계;를 포함하는 미백 및 항산화기능을 가지는 화장품 조성물의 제조방법을 제공한다.Glutathione 0.3-0.7 parts by weight, tocopherol 0.5-2 parts by weight, 0.5-3 parts by weight of the above natural complex extract, ascorbic acid 9-20 parts by weight, ethyl ascorbyl ether 1-2 parts by weight, and tranexamic acid 1-3 It provides a method for producing a cosmetic composition having whitening and antioxidant functions, comprising the step of producing a composition containing parts by weight.
본 발명에 따른 화장품 조성물은 항산화 활성 및 미백기능을 가진다.The cosmetic composition according to the present invention has antioxidant activity and whitening function.
도 1은 본 발명에 따른 일 측면에서 제공되는 제1 천연추출물의 제조방법을 순서도로 나타낸 것이고,Figure 1 shows a flow chart of a method for producing a first natural extract provided in one aspect according to the present invention,
도 2는 본 발명에 따른 일 측면에서 제공되는 제2 천연추출물의 제조방법을 순서도로 나타낸 것이고,Figure 2 is a flowchart showing a method for producing a second natural extract provided in one aspect according to the present invention;
도 3은 본 발명에 따른 일 측면에서 제공되는 제3 천연추출물의 제조방법을 순서도로 나타낸 것이고,Figure 3 is a flowchart showing a method for producing a third natural extract provided in one aspect according to the present invention;
도 4는 스킨제형의 실시예 및 비교예의 전자공여능 측정 시험 결과를 나타낸 것이고,Figure 4 shows the electron donating ability measurement test results of examples and comparative examples of skin formulations;
도 5는 로션제형의 실시예 및 비교예의 전자공여능 측정 시험 결과를 나타낸 것이고, Figure 5 shows the electron donating ability measurement test results of Examples and Comparative Examples of lotion formulations;
도 6은 세럼제형의 실시예 및 비교예의 전자공여능 측정 시험 결과를 나타낸 것이고,Figure 6 shows the electron donating ability measurement test results of Examples and Comparative Examples of serum formulations;
도 7은 크림제형의 실시예 및 비교예의 전자공여능 측정 시험 결과를 나타낸 것이고,Figure 7 shows the electron donating ability measurement test results of Examples and Comparative Examples of cream formulations;
도 8은 스킨제형의 실시예 및 비교예의 Tyrosinase 저해 활성 측정 시험 결과를 나타낸 것이고,Figure 8 shows the results of a test for measuring Tyrosinase inhibitory activity of examples and comparative examples of skin formulations;
도 9는 로션제형의 실시예 및 비교예의 Tyrosinase 저해 활성 측정 시험 결과를 나타낸 것이고,Figure 9 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of lotion formulations.
도 10은 세럼제형의 실시예 및 비교예의 Tyrosinase 저해 활성 측정 시험 결과를 나타낸 것이고,Figure 10 shows the results of a test for measuring Tyrosinase inhibitory activity of examples and comparative examples of serum formulations;
도 11은 크림제형의 실시예 및 비교예의 Tyrosinase 저해 활성 측정 시험 결과를 나타낸 것이다.Figure 11 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of cream formulations.
이하에서는 첨부된 도면을 참조하여 다양한 실시예를 보다 상세하게 설명한다. 본 명세서에 기재된 실시예는 다양하게 변형될 수 있다. 특정한 실시예가 도면에서 묘사되고 상세한 설명에서 자세하게 설명될 수 있다. 그러나 첨부된 도면에 개시된 특정한 실시 예는 다양한 실시예를 쉽게 이해하도록 하기 위한 것일 뿐이다. 따라서 첨부된 도면에 개시된 특정 실시예에 의해 기술적 사상이 제한되는 것은 아니며, 발명의 사상 및 기술 범위에 포함되는 모든 균등물 또는 대체물을 포함하는 것으로 이해되어야 한다.Hereinafter, various embodiments will be described in more detail with reference to the attached drawings. The embodiments described herein may be modified in various ways. Specific embodiments may be depicted in the drawings and described in detail in the detailed description. However, the specific embodiments disclosed in the attached drawings are only intended to facilitate understanding of the various embodiments. Accordingly, the technical idea is not limited to the specific embodiments disclosed in the attached drawings, and should be understood to include all equivalents or substitutes included in the spirit and technical scope of the invention.
1차, 2차, 제1, 제2 등과 같이 서수를 포함하는 용어는 다양한 구성요소들을 설명하는데 사용될 수 있지만, 이러한 구성요소들은 상술한 용어에 의해 한정되지는 않는다. 상술한 용어는 하나의 구성요소를 다른 구성요소로부터 구별하는 목적으로만 사용된다.Terms containing ordinal numbers, such as primary, secondary, first, second, etc., may be used to describe various components, but these components are not limited by the above-mentioned terms. The above-mentioned terms are used only for the purpose of distinguishing one component from another.
본 명세서에서, '포함한다' 또는 '가지다' 등의 용어는 명세서상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다. 어떤 구성요소가 다른 구성요소에 '연결되어' 있다거나 '접속되어' 있다고 언급된 때에는, 그 다른 구성요소에 직접적으로 연결되어 있거나 또는 접속되어 있을 수도 있지만, 중간에 다른 구성요소가 존재할 수도 있다고 이해되어야 할 것이다. 반면에, 어떤 구성요소가 다른 구성요소에 '직접 연결되어' 있다거나 '직접 접속되어' 있다고 언급된 때에는, 중간에 다른 구성요소가 존재하지 않는 것으로 이해되어야 할 것이다.In this specification, terms such as 'include' or 'have' are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that this does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof. When a component is said to be 'connected' or 'connected' to another component, it is understood that it may be directly connected or connected to the other component, but that other components may exist in between. It should be. On the other hand, when a component is mentioned as being 'directly connected' or 'directly connected' to another component, it should be understood that there are no other components in between.
그 밖에도, 본 발명을 설명함에 있어서, 관련된 공지 기능 혹은 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우, 그에 대한 상세한 설명은 축약하거나 생략한다.In addition, when describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof is abbreviated or omitted.
본 발명은This invention
글루타치온, 토코페롤, 천연복합추출물, 아스코빅애씨드, 에칠아스코빌에텔 및 트라넥사믹애씨드를 포함하는 미백 및 항산화기능을 가지는 화장품 조성물을 제공한다.Provides a cosmetic composition with whitening and antioxidant functions containing glutathione, tocopherol, natural complex extract, ascorbic acid, ethyl ascorbyl ether, and tranexamic acid.
이하, 본 발명에 따른 항산화 활성 및 미백기능을 가지는 화장품 조성물에 대하여 상세히 설명한다.Hereinafter, the cosmetic composition having antioxidant activity and whitening function according to the present invention will be described in detail.
상기 글루타치온(Glutathione)은 간에서 생성되는 물질로 세포와 조직 기관을 활성 산소로부터 보호해주는 단백질의 일종이다. 항산화 효과가 뛰어나 피부 속 멜라닌 생성을 억제해 미백 효과에 도움을 주게 된다. 상기 글루타치온은 0.3-1 중량부를 포함하는 것이 바람직하고, 0.3-0.7 중량부를 포함하는 것이 더욱 바람직하다. 상기 함량 범위에서 적게 사용하는 경우 항산화 활성 및 미백 효과가 거의 없는 문제가 있으며, 또한 과량 사용하는 경우 오히려 피부가 붉어지는 등의 부작용이 발생하는 문제가 있다.Glutathione is a substance produced in the liver and is a type of protein that protects cells and tissues from active oxygen. It has an excellent antioxidant effect and suppresses melanin production in the skin, contributing to the whitening effect. The glutathione preferably contains 0.3-1 part by weight, and more preferably 0.3-0.7 parts by weight. If used in a small amount within the above content range, there is a problem that there is almost no antioxidant activity and whitening effect, and if used in excessive amounts, there is a problem that side effects such as skin redness occur.
상기 토코페롤(tocopherol)은 지용성 비타민으로 세포의 이중 지질막에 쉽게 침투할 수 있어 표피 조직의 멜라노사이트 내에서 효과적으로 항산화 활성 및 멜라닌 생합성 저해 작용을 나타내고, 수용성 아스코빅애씨드의 우수한 항산화 효과, 타이로시나아제 활성 억제 효과, 멜라닌 생합성 저해 효과를 극대화함으로써 피부의 색소 침착을 효과적으로 예방해 주며, 피부색 개선 및 피부 광택 등의 우수한 미백 효과를 나타낸다. 상기 토코페롤은 0.5-3 중량부를 포함하는 것이 바람직하고, 0.5-2 중량부를 포함하는 것이 더욱 바람직하다. 상기 함량 범위에서 적게 사용하는 경우 항산화 활성이 미미한 문제가 있으며, 또한 과량 사용하는 경우 피부가 오히려 건조해지거나 가려운 부작용이 발생하는 문제가 있다.The tocopherol is a fat-soluble vitamin that can easily penetrate the double lipid membrane of cells, effectively exhibiting antioxidant activity and inhibiting melanin biosynthesis within melanocytes of epidermal tissue, and the excellent antioxidant effect of water-soluble ascorbic acid and tyrosinase. By maximizing the activity inhibition effect and melanin biosynthesis inhibition effect, it effectively prevents skin pigmentation and has excellent whitening effects such as improving skin color and skin gloss. The tocopherol preferably contains 0.5-3 parts by weight, and more preferably 0.5-2 parts by weight. If used in a small amount within the above content range, there is a problem that the antioxidant activity is minimal, and if used in excessive amounts, there is a problem that the skin becomes dry or itchy side effects occur.
상기 아스코빅애씨드는 비타민 C라고도 하며, 효과는 항산화, 콜라겐 합성, 미백 3가지로 구분할 수 있다. 자외선은 피부에 가장 많은 영향을 미치는 데, 자외선에 의해 피부는 늘 손상을 받으며, 이러한 자외선에 의해 발생되는 활성산소는 피부노화의 주범으로 아스코빅애씨드는 활성산소의 작용을 억제하는 항산화제로서 노화방지효과를 나타낸다. 그리고 진피의 주성분인 콜라겐 합성에 보조역할을 함으로서 주름개선과 예방효과를 지닌다. 또한, 자외선 노출과 정상적인 노화에 의해 야기된 불균일한 색소 침착을 완화시키는 데, 멜라닌 생성을 억제하고 산화된 멜라닌을 환원하여 색소 침착을 예방하며 기미, 주근깨 치료 등에 사용될 수 있다. 상기 아스코빅애씨드는 9-25 중량부를 포함하는 것이 바람직하고, 9-20 중량부를 포함하는 것이 더욱 바람직하다. 상기 함량 범위에서 적게 사용하는 경우 항산화 활성 및 미백 효과가 거의 없는 문제가 있으며, 또한, 과량 사용하는 경우 피부로 흡수되기 어려운 문제가 있으며 민감성 피부에 적용시 피부에 대한 안전성이 감소하는 문제가 있다.Ascorbic acid is also called vitamin C, and its effects can be divided into three categories: antioxidant, collagen synthesis, and whitening. Ultraviolet rays have the greatest impact on the skin. The skin is always damaged by ultraviolet rays, and the free radicals generated by these ultraviolet rays are the main cause of skin aging. Ascorbic acid is an antioxidant that inhibits the action of free radicals and causes aging. It shows a preventive effect. In addition, it has the effect of improving and preventing wrinkles by playing an auxiliary role in the synthesis of collagen, the main component of the dermis. In addition, it can be used to alleviate uneven pigmentation caused by exposure to ultraviolet rays and normal aging, prevent pigmentation by suppressing melanin production and reduce oxidized melanin, and treat blemishes and freckles. The ascorbic acid preferably contains 9-25 parts by weight, and more preferably contains 9-20 parts by weight. When used in a small amount within the above range, there is a problem of almost no antioxidant activity and whitening effect. Additionally, when used in excessive amounts, it is difficult to be absorbed into the skin, and when applied to sensitive skin, the safety for the skin is reduced.
상기 에칠아스코빌에텔(Ethyl Ascorbyl Ether)은 아스코빅애씨드로부터 합성된 물질 또는 유도체로 피부노화, 주름개선, 미백기능이 우수하여 아스코빅애씨드와 같이 적용되어 아스코빅애씨드의 불안정성의 단점을 극복하여 더욱 높은 미백 기능 개선 효과를 나타낼 수 있다. 더욱이, 이를 적용하는 경우 피부자극이 없으면서도 더욱 우수한 미백 기능을 나타낸다. 상기 에칠아스코빌에텔는 0.5-4 중량부를 포함하는 것이 바람직하고, 1-2 중량부를 포함하는 것이 더욱 바람직하다. 상기 함량 범위에서 적게 사용하는 경우 항산화 활성 및 미백 효과가 거의 없는 문제가 있으며, 또한 과량 사용하는 경우 피부로 흡수되기 어려운 문제가 있으며 민감성 피부에 적용시 피부에 대한 안전성이 감소하는 문제가 있다.Ethyl Ascorbyl Ether is a substance or derivative synthesized from ascorbic acid and has excellent skin aging, wrinkle improvement, and whitening functions, so it is applied together with ascorbic acid to overcome the disadvantage of ascorbic acid's instability. It can show a high whitening function improvement effect. Moreover, when applied, it shows superior whitening function without skin irritation. The ethyl ascorbyl ether preferably contains 0.5-4 parts by weight, and more preferably 1-2 parts by weight. If used less than the above content range, there is a problem of almost no antioxidant activity and whitening effect, and if used in excessive amounts, there is a problem that it is difficult to be absorbed into the skin, and when applied to sensitive skin, the safety for the skin is reduced.
상기 트라넥사믹애씨드(tranexamic acid)는 합성 아미노산으로 아미노산인 리신(lysine)에서 유래되는 흰색의 결정체이다. 원래 항염증, 항알레르기 지혈효과 등이 있어서 의약분야에 사용되는 물질로 습진, 두드러기, 편도염, 지혈제 등 폭넓게 사용되며 안정성이 확인된 성분이다. 이를 화장품 조성물에 적용하여 피부 장벽을 복구하고 손상된 피부를 회복시키는 기능과 더불어 피부 미백 기능을 높일 수 있다. 상기 트라넥사믹애씨드는 1-4 중량부를 포함하는 것이 바람직하고, 1-3 중량부를 포함하는 것이 더욱 바람직하다.The tranexamic acid is a synthetic amino acid and is a white crystal derived from the amino acid lysine. Originally, it is a substance used in the medical field due to its anti-inflammatory, anti-allergic and hemostatic effects. It is widely used for eczema, urticaria, tonsillitis, and hemostatic agents, and its safety has been confirmed. By applying this to cosmetic compositions, it can improve the skin whitening function as well as the function of repairing the skin barrier and restoring damaged skin. The tranexamic acid preferably contains 1-4 parts by weight, and more preferably 1-3 parts by weight.
상기 천연복합추출물은 연꽃씨추출물, 인동덩굴꽃추출물, 우엉씨추출물, 지모뿌리추출물, 당개나리열매추출물, 프로폴리스추출물, 병풀추출물 및 길경추출물을 포함한다. 상기 천연복합추출물은 0.5-5 중량부를 포함하는 것이 바람직하고, 0.5-3 중량부를 포함하는 것이 더욱 바람직하다.The natural complex extract includes lotus seed extract, honeysuckle flower extract, burdock seed extract, Gimo root extract, Forsythia fruit extract, propolis extract, centella asiatica extract, and Gilge extract. The natural complex extract preferably contains 0.5-5 parts by weight, and more preferably contains 0.5-3 parts by weight.
상기 연꽃씨(Nelumbo Nucifera Seed)는 연꽃의 씨앗을 말한다. 연꽃은 미나리아제비목 수련과의 여러해살이 수초로 꽃은 7-8월에 피고 홍색 또는 백색이며 꽃줄기 끝에 1개씩 달리고 꽃턱의 구멍에 씨앗이 있다. 연은 다양한 부위가 식품 또는 한약재로 오랫동안 사용되어 오면서 그 안전성이 입증되었다. 이러한 연꽃씨추출물은 화장품 성분으로 사용될 경우, 아스파라긴 및 알기닌이 함유되어 있어 피부노화에 치명적인 자유라디칼을 제거 할 수 있고, 칙칙한 피부톤을 맑고 화사하게 가꾸어 주는데 도움이 된다. 또한 피부를 진정시키며 피부의 과산화를 억제해 피부가 받는 산화적 스트레스를 줄여주는데 도움을 줄 수 있다.The lotus seed (Nelumbo Nucifera Seed) refers to the seed of the lotus flower. The lotus is a perennial aquatic plant of the order Ranunculaceae and the water lily family. The flowers bloom in July-August, are red or white, have one flower at the end of the flower stem, and have seeds in the holes of the flower stalk. Its safety has been proven as various parts of lotus have been used as food or herbal medicine for a long time. When used as a cosmetic ingredient, lotus seed extract contains asparagine and arginine, which can remove free radicals that are fatal to skin aging and help make dull skin tone clear and bright. It can also help soothe the skin and reduce oxidative stress on the skin by suppressing skin peroxidation.
상기 인동덩굴꽃(Lonicera Japonica Flower)은 인동덩굴의 꽃을 말한다. 우리나라 전역의 산에서 자라는 반상록활엽 덩굴성 관목으로, 한약재명으로는 금은화라고 한다. 이러한 인동덩굴은 일반적으로 고열, 급성 간염, 염증 등에 약용으로 사용되어 왔다. 이러한 인동덩굴꽃추출물은 화장품 성분으로 사용될 경우, 진놀, 스테롤, 글리코시드, 클로로겐산, 플라보노이드 화합물, 탄닌, 이소클로로겐산, 카페익산, 케르세틴 등의 성분을 함유하고 있어 항산화 작용을 높여 피부 노화를 방지하고 아스코빅애씨드 등의 미백 성분을 보조하여 미백 기능을 극대화시킨다. The Lonicera Japonica Flower refers to the flower of the honeysuckle vine. It is a semi-evergreen broad-leafed vine shrub that grows in the mountains throughout Korea, and is called honeysuckle in herbal medicine. These honeysuckle plants have generally been used medicinally for high fever, acute hepatitis, and inflammation. When used as a cosmetic ingredient, honeysuckle flower extract contains ingredients such as ginol, sterols, glycosides, chlorogenic acid, flavonoid compounds, tannin, isochlorogenic acid, caffeic acid, and quercetin. It maximizes whitening function by assisting with whitening ingredients such as big acid.
상기 우엉씨(Arctium Lappa Seed)는 우엉의 씨앗을 말한다. 우엉은 우엉은 높이 50-150 cm이다. 곧은 뿌리가 30-60 cm 자라고 끝에서 줄기가 나온다. 뿌리에 달린 잎은 무더기로 나오고 잎자루가 길다. 줄기에서는 어긋나며 심장모양이다. 겉면은 짙은 녹색이지만 뒷면에 흰 솜털이 빽빽이 나며, 가장자리에 이 모양의 톱니가 있다. 꽃은 7-8월에 피는데 검은 자줏빛이 돌며, 두화는 가지 끝에 산방꽃차례로 달린다. 총포는 둥글고 포는 바늘 모양이며 끝이 갈고리처럼 생긴다. 꽃은 관상화이고 종자는 검은색이며 관모는 갈색이다. 열매는 수과로서 9월에 익는다. 강건하여 병이 거의 없고 추위에도 매우 강하며 토질을 별로 가리지 않는다. 번식은 종자나 포기나누기로 한다. 유럽 원산의 귀화식물이다. 품종으로는 뿌리가 길고 굵은 노야천과 육질이 좋고 뿌리가 짧은 사천 등이 있다. 뿌리에는 이눌린과 약간의 팔미트산이 들어있다. 유럽에서는 이뇨제와 발한제로 쓰고 종자는 부기가 있을 때 이뇨제로 사용하며, 인후통과 독충의 해독제로 쓴다. 우엉씨는 우방자라고도 하며, 풍열을 소통시켜 흩어지게 하고 폐기를 통하게 하며 두진이 돋아나게 하고 부기를 가라앉히며 해독하는 효능이 있는 약재로도 쓰인다. 이러한 우엉씨추출물은 화장품 성분으로 사용될 경우, 악티게닌, 비타민 A, 비타민 B1 등을 포함하고 있어 멜라닌 생성 억제 효과를 나타내며 미백 효과를 가진다. The above burdock seeds (Arctium Lappa Seed) refer to the seeds of burdock. Burdock is 50-150 cm tall. The taproot grows 30-60 cm long and a stem emerges from the end. The leaves attached to the roots come out in clusters and have long petioles. The stems are alternate and heart-shaped. The outside is dark green, but the back has dense white fluff, and there are teeth shaped like this on the edges. The flowers bloom in July-August and are black-purple, and the flower heads hang in corymbs at the ends of branches. The involucre is round, the bracts are needle-shaped, and the ends look like hooks. The flowers are tubular, the seeds are black, and the tubular hairs are brown. The fruit is an achene and ripens in September. It is hardy, rarely suffers from diseases, is very resistant to cold, and is not particularly sensitive to soil type. Propagation is done by seed or by dividing. It is a naturalized plant native to Europe. Varieties include Noyacheon, which has long and thick roots, and Sacheon, which has good flesh and short roots. The root contains inulin and some palmitic acid. In Europe, it is used as a diuretic and diaphoretic, and the seeds are used as a diuretic when there is swelling, and as an antidote to sore throats and poisonous insects. Burdock seeds are also called Ubangja, and are also used as a medicinal herb that has the effect of dispersing wind heat by dissipating it, clearing waste, causing acne to grow, reducing swelling, and detoxifying. When used as a cosmetic ingredient, burdock seed extract contains arctigenin, vitamin A, vitamin B1, etc., which inhibits melanin production and has a whitening effect.
상기 지모뿌리(Anemarrhena Asphodeloides Root)는 지모의 뿌리를 말한다. 지모는 백합과에 속하는 여러살이 풀로 지모의 뿌리에는 인삼의 주요원료와 동일한 사포닌이 다량 함유되어 있으며 약재의 용도로 사용되고 있으며, 열로 인한 기침, 담, 갈증 개선에 도움을 준다. 이러한 지모뿌리추출물은 화장품 성분으로 사용될 경우 보르피린, 사포닌 등이 함유되어 있어 피부톤 개선, 피부 진정에 도움을 주고, 보습 강화, 독소 배출, 항산화 효과를 나타낸다.The Anemarrhena Asphodeloides Root refers to the root of the Anemarrhena Asphodeloides Root. Jimo is a perennial herb belonging to the Liliaceae family. Its roots contain a large amount of saponin, the same as the main ingredient of ginseng, and are used for medicinal purposes. It helps improve cough, phlegm, and thirst caused by fever. When used as a cosmetic ingredient, this zygote root extract contains borphyrin, saponin, etc., which helps improve skin tone, soothe the skin, enhances moisturizing, releases toxins, and has antioxidant effects.
상기 당개나리열매(Forsythia Suspensa Fruit)는 당개나리의 열매를 말한다. 당개나리는 연교라고도 하며, 물푸레나무과의 의성개나리의 열매를 약용한 것으로 특이한 냄새가 있으며 맛은 쓰고 성질은 약간 차다. 또한 열을 내리고 해독하므로 온열병 초기에 심장의 열을 내리고 고열과 정신혼몽에 쓰며, 종기, 반진, 맹장염, 폐녹양, 림프절염, 인후염 등에 사용하고 이뇨, 소염효과가 있다. 약리작용으로 항균작용, 항염증작용, 혈압강하, 지혈작용, 간치료작용, 해열, 진통, 이뇨작용이 보고되었다. 이러한, 당개나리열매추출물은 화장품 성분으로 사용될 경우 사포닌, 올레아놀산, 악티게닌, 마타이레시놀글루코사이드 등의 성분을 함유하여 피부를 촉촉하게 유지하도록 보습력 향상에 도움을 주며, 항산화효과로 유해물질로부터 피부를 보호한다.The Forsythia Suspensa Fruit refers to the fruit of Forsythia Suspensa. Forsythia is also called Yeongyo. It is a medicinal product of the fruit of Uiseong Forsythia, a member of the ash tree family. It has a unique smell, tastes bitter, and is slightly cold in nature. In addition, it reduces fever and detoxifies, so it reduces heart heat in the early stage of heat illness, is used for high fever and mental confusion, and is used for boils, rashes, appendicitis, pulmonary rust, lymphadenitis, and sore throat, and has a diuretic and anti-inflammatory effect. Antibacterial, anti-inflammatory, blood pressure lowering, hemostatic, liver healing, antipyretic, analgesic, and diuretic effects have been reported as pharmacological effects. When used as a cosmetic ingredient, forsythia fruit extract contains ingredients such as saponin, oleanolic acid, arctigenin, and matairesinol glucoside, which helps improve moisturizing power to keep the skin moist, and protects the skin from harmful substances with its antioxidant effect. protect
상기 프로폴리스(Propolis)는 이미,이취가 없고 고유의 향미가 있는 어두운 회갈색의 분말로, 꿀벌이 자신의 생존과 번식을 위해 여러 식물에서 뽑아낸 수지와 같은 물질에 자신의 침과 효소 등을 섞어서 만든 물질로서, 성분으로는 유기물과 미네랄(무기염류)이 가장 많은데, 미네랄, 비타민, 아미노산, 지방, 유기산, 플라보노이드 등은 세포대사에 중요한 역할을 하며, 테르펜류 등은 항암 작용을 한다. 주요한 효능으로는 항염, 항산화, 면역증강, 보습 등이 있다. 이러한 프로폴리스추출물은 화장품 성분으로 사용될 경우, 20종이 넘는 다양한 플라보니이드를 함유하고 있어 활성산소로부터 피부를 보호하는 항산화 역할을 극대화시켜 준다.Propolis is a dark gray-brown powder that has no unpleasant odor and has a unique flavor. Bees mix their own saliva and enzymes with substances such as resins extracted from various plants for their own survival and reproduction. As a made substance, the most common ingredients are organic substances and minerals (inorganic salts). Minerals, vitamins, amino acids, fats, organic acids, flavonoids, etc. play an important role in cell metabolism, and terpenes have anti-cancer properties. Its main effects include anti-inflammatory, antioxidant, immune enhancement, and moisturizing properties. When used as a cosmetic ingredient, this propolis extract contains over 20 different types of flavonoids, maximizing its antioxidant role in protecting the skin from free radicals.
상기 길경(Platycodon Grandiflorus)은 길경근으로도 불리며 초롱꽃과 도라지의 뿌리 또는 주피를 제거하여 만든 약재로서, 냄새가 약간 있고 맛은 쓰고 매우며 성질은 평하다고 알려져 있다. 길경은 폐에 작용하여 해수와 가래가 많고 호흡이 불편한 증상을 치료하며 기침을 멈추고 담을 없애는 효과가 있다. 또한, 소변을 잘 보지 못하여 전신부종이 있고 소변양이 적을 때도 사용한다. 따라서 인후통, 감기로 인한 기침, 가래, 코막힘, 천식, 기관지염증, 흉막염, 두통, 오한, 편도선염 등에 처방되어 사용한다. 약리작용으로는 거담작용, 콜레스테롤 강하작용, 및 개선균 억제작용 등이 보고되어있다. 이러한 길경추출물은 화장품 성분으로 사용될 경우 3''-O-아세틸플라티코닉애씨드A, 3''-O-아세틸폴리갈락신 D, 폴리갈락신D3 등의 성분을 함유하여 화장품의 산화를 억제하여 화장품의 변질, 부패, 품질저하 등을 방지하고, 피부에 해로운 외부 물질의 활성을 방해한다.Platycodon Grandiflorus, also called Gilgyeonggeun, is a medicinal herb made by removing the roots or pericarp of campanula and bellflower root, and is known to have a slight odor, bitter taste, and flat properties. Gilgyeong acts on the lungs to treat symptoms such as excessive seawater and phlegm and difficulty breathing, and has the effect of stopping coughing and removing phlegm. It is also used when there is general edema and small amount of urine due to difficulty in urinating. Therefore, it is prescribed and used for sore throat, cough due to cold, phlegm, nasal congestion, asthma, bronchitis, pleurisy, headache, chills, tonsillitis, etc. Pharmacological effects include expectorant action, cholesterol-lowering action, and antibacterial action have been reported. When used as a cosmetic ingredient, this Gilge extract contains ingredients such as 3''-O-acetylplaticonic acid A, 3''-O-acetylpolygalaxin D, and polygalaxin D3, thereby suppressing oxidation of cosmetics. It prevents deterioration, decay, and deterioration of cosmetics, and hinders the activity of external substances harmful to the skin.
상기 병풀은 센텔라아시아티카이며 가느다란 다년생의 덩굴식물로서 열대 지방에서 자생한다. 예로부터 호랑이가 몸에 상처가 났을 때 이 풀 밭에서 뒹굴었다고 하여 호랑이풀이라고도 하며 특히 잎과 줄기에 아시아티코사이드, 사포닌, 마테카식에씨드와 같은 유효성분을 포함하고 있어 매끈한 피부를 가꾸는데 도움을 준다. 이러한 병풀추출물은 화장품 성분으로 사용될 경우 멜라닌을 억제하여 자극 받은 피부를 진정시켜 주고 색소 침착을 방지하여 미백 기능을 보조하여 준다.The centella asiatica is a slender perennial vine that grows naturally in tropical regions. It is also called tiger grass because it is said that tigers used to roll in this grass field when they had injuries on their bodies since ancient times. In particular, the leaves and stems contain active ingredients such as asiaticoside, saponin, and matecasque acid, which helps in maintaining smooth skin. gives. When used as a cosmetic ingredient, this Centella asiatica extract soothes irritated skin by suppressing melanin and prevents pigmentation to assist with whitening function.
또한, 본 발명에 따른 미백 및 항산화기능을 가지는 화장품 조성물은 부틸렌글라이콜 1-6 중량부, 트로메타민 1-3 중량부, 프로판다이올 1-6 중량부, 암모늄아크릴로일다이메틸타우레이트/브이피코폴리머 0.1-1 중량부, 1,2-헥산다이올 1-3 중량부 및 나머지 잔부로 물을 포함하는 것이 바람직하고, 다이프로필렌글라이콜 1-6 중량부, 부틸렌글라이콜 1-6 중량부, 프로판다이올 1-6 중량부, 트로메타민 0.5-3 중량부, 잔탄검 0.1-0.5 중량부, 암모늄아크릴로일다이메틸타우레이트/브이피코폴리머 0.1-1 중량부, 폴리소르베이트80 0.1-2 중량부, 다이카프릴릴카보네이트 1-8 중량부, 다이메치콘,다이메티콘/비닐다이메티콘크로스폴리머 0.1-2 중량부, 1,2-헥산다이올 1-3 중량부, 에칠헥실글리세린 0.01-0.1 중량부 및 나머지 잔부로 물을 포함하는 것이 바람직하고, 부틸렌글라이콜 1-6 중량부, 프로판다이올 1-6 중량부, 소듐락테이트 1-5중량부, 1,2-헥산다이올 1-3 중량부, 에칠헥실글리세린 0.01-0.1 중량부, 다이프로필렌글라이콜 1-6중량부, 라우레스-23 1-5 중량부, 소듐하이알루로네이트 0.1-1 중량부 및 나머지 잔부로 물을 포함하는 것이 바람직하고, 다이프로필렌글라이콜 1-6 중량부, 부틸렌글라이콜 1-6 중량부, 프로판다이올 1-6 중량부, 트로메타민 0.5-3 중량부, 잔탄검 0.1-0.5 중량부, 암모늄아크릴로일다이메틸타우레이트/브이피코폴리머 0.1-1 중량부, 폴리소르베이트80 0.1-2 중량부, 세테아릴알코올 1-5 중량부, 다이카프릴릴카보네이트 1-8 중량부, 다이메치콘,다이메티콘/비닐다이메티콘크로스폴리머 0.1-2 중량부, 1,2-헥산다이올 1-3 중량부, 에칠헥실글리세린 0.01-0.1 중량부 및 나머지 잔부로 물을 포함하는 것이 바람직하다.In addition, the cosmetic composition having whitening and antioxidant functions according to the present invention contains 1-6 parts by weight of butylene glycol, 1-3 parts by weight of tromethamine, 1-6 parts by weight of propanediol, and ammonium acryloyldimethyl tau. It is preferable to include 0.1-1 part by weight of rate/VP copolymer, 1-3 parts by weight of 1,2-hexanediol, and the remainder containing water, and 1-6 parts by weight of dipropylene glycol and butylene glycol. 1-6 parts by weight, propanediol 1-6 parts by weight, tromethamine 0.5-3 parts by weight, xanthan gum 0.1-0.5 parts by weight, ammonium acryloyldimethyl taurate/VPicopolymer 0.1-1 part by weight, 0.1-2 parts by weight of polysorbate 80, 1-8 parts by weight of dicaprylyl carbonate, 0.1-2 parts by weight of dimethicone, dimethicone/vinyl dimethicone crosspolymer, 1,2-hexanediol 1- It is preferable to include 3 parts by weight, 0.01-0.1 parts by weight of ethylhexylglycerin, and the remainder containing water, 1-6 parts by weight of butylene glycol, 1-6 parts by weight of propanediol, and 1-5 parts by weight of sodium lactate. parts, 1-3 parts by weight of 1,2-hexanediol, 0.01-0.1 parts by weight of ethylhexylglycerin, 1-6 parts by weight of dipropylene glycol, 1-5 parts by weight of Laureth-23, sodium hyaluronate It is preferable to include 0.1-1 part by weight and the remainder of water, 1-6 parts by weight of dipropylene glycol, 1-6 parts by weight of butylene glycol, 1-6 parts by weight of propanediol, and tromethamine. 0.5-3 parts by weight, xanthan gum 0.1-0.5 parts by weight, ammonium acryloyldimethyl taurate/VPicopolymer 0.1-1 parts by weight, polysorbate 80 0.1-2 parts by weight, cetearyl alcohol 1-5 parts by weight parts, dicaprylyl carbonate 1-8 parts by weight, dimethicone, dimethicone/vinyl dimethicone crosspolymer 0.1-2 parts by weight, 1,2-hexanediol 1-3 parts by weight, ethylhexylglycerin 0.01 It is preferable to include water at -0.1 part by weight and the remaining balance.
또한, 본 발명은 In addition, the present invention
연꽃씨 및 우엉씨를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 연꽃씨분말 및 우엉씨분말을 포함하는 제1 혼합분말을 준비하는 단계; 상기 제1 혼합분말, 1:1의 부피비로 혼합된 아세톤 및 클로로포름의 혼합 용매 및 1-에틸-3-메틸이미다졸리움 비스(트리플루오로메틸설포닐)이미드(EMIM TFSI, 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfylimide)를 혼합하여 유효성분을 포함하는 용액을 추출하는 단계; 및 추출된 용액을 감압농축 및 동결건조하는 단계;를 포함하는 연꽃씨추출물 및 우엉씨추출물을 포함하는 제1 천연추출물을 제조하는 단계;Washing lotus seeds and burdock seeds, cutting them into appropriate sizes, freeze-drying, and pulverizing them to prepare a first mixed powder containing lotus seed powder and burdock seed powder; The first mixed powder, a mixed solvent of acetone and chloroform mixed at a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMIM TFSI, 1-Ethyl- The first natural product comprising lotus seed extract and burdock seed extract, comprising the steps of extracting a solution containing the active ingredient by mixing 3-methylimidazolium bis (trifluoromethylsulfylimide); and concentrating and freeze-drying the extracted solution under reduced pressure. Preparing an extract;
인동덩굴꽃, 지모뿌리 및 당개나리열매를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 인동덩굴꽃분말, 지모뿌리분말 및 당개나리열매분말을 포함하는 제2 혼합분말을 준비하는 단계; 상기 제2 혼합분말 및 물을 혼합하고, 교반을 수행하며 95-105℃의 온도에서 10-14시간 동안 1차 열수추출을 수행하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 135-145℃의 온도에서 4-8시간 동안 2차 열수추출을 수행하는 단계; 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조하는 단계; 및 상기 제1 추출물 및 제2 추출물을 혼합하는 단계;를 포함하는 인동덩굴꽃추출물, 지모뿌리추출물 및 당개나리열매추출물을 포함하는 제2 천연추출물을 제조하는 단계;Preparing a second mixed powder containing honeysuckle flower powder, root root powder, and forsythia fruit powder by washing honeysuckle flowers, root roots, and forsythia fruit, cutting them into appropriate sizes, freeze-drying, and pulverizing them; mixing the second mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105°C for 10-14 hours; After performing the first hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering through filter paper, mixing the remaining solids with water again, and performing the second hot water extraction at a temperature of 135-145°C for 4-8 hours; After secondary hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a second extract; and mixing the first extract and the second extract; preparing a second natural extract comprising a honeysuckle flower extract, a root extract, and a forsythia fruit extract;
프로폴리스, 병풀 및 길경을 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 프로폴리스분말, 병풀분말 및 길경분말을 포함하는 제3 혼합분말을 준비하는 단계; 상기 제3 혼합분말 및 에탄올을 혼합하여 유효성분을 포함하는 용액을 추출하는 단계; 및 추출된 용액을 감압농축 및 동결건조하는 단계;를 포함하는 프로폴리스추출물, 병풀추출물 및 길경추출물을 포함하는 제3 천연추출물을 제조하는 단계;Preparing a third mixed powder containing propolis powder, centella asiatica powder, and apricot root by washing propolis, centella asiatica, and apricot root, cutting them into appropriate sizes, freeze-drying, and pulverizing them; extracting a solution containing the active ingredient by mixing the third mixed powder and ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure; preparing a third natural extract including propolis extract, Centella asiatica extract, and Gilge extract;
상기 제1 천연추출물 28-32 중량부, 상기 제2 천연추출물 28-32 중량부 및 상기 제3 천연추출물 38-42 중량부를 혼합하여 천연복합추출물을 제조하는 단계; 및Preparing a natural complex extract by mixing 28-32 parts by weight of the first natural extract, 28-32 parts by weight of the second natural extract, and 38-42 parts by weight of the third natural extract; and
글루타치온 0.3-0.7 중량부, 토코페롤 0.5-2 중량부, 상기 천연복합추출물 0.5-3 중량부, 아스코빅애씨드 9-20 중량부, 에칠아스코빌에텔 1-2 중량부 및 트라넥사믹애씨드 1-3 중량부를 포함하는 조성물을 제조하는 단계;를 포함하는 미백 및 항산화기능을 가지는 화장품 조성물의 제조방법을 제공한다.Glutathione 0.3-0.7 parts by weight, tocopherol 0.5-2 parts by weight, 0.5-3 parts by weight of the above natural complex extract, ascorbic acid 9-20 parts by weight, ethyl ascorbyl ether 1-2 parts by weight, and tranexamic acid 1-3 It provides a method for producing a cosmetic composition having whitening and antioxidant functions, comprising the step of producing a composition containing parts by weight.
이때, 도 1 내지 도 3에 본 발명에 따른 일 측면에서 제공되는 천연추출물의 제조방법을 순서도로 나타내었으며, 이하, 도 1 내지 도 3을 참조하여 본 발명에 따른 미백 및 항산화기능을 가지는 화장품 조성물의 제조방법에 대하여 각 단계별로 상세히 설명한다.At this time, Figures 1 to 3 show a flow chart of the method for producing the natural extract provided in one aspect according to the present invention. Hereinafter, with reference to Figures 1 to 3, the cosmetic composition having whitening and antioxidant functions according to the present invention. The manufacturing method is explained in detail at each step.
먼저, 본 발명에 따른 미백 및 항산화기능을 가지는 화장품 조성물의 제조방법은 연꽃씨추출물 및 우엉씨추출물을 포함하는 제1 천연추출물을 제조하는 단계를 포함한다. 상기 제1 천연추출물을 제조하는 단계는 연꽃씨 및 우엉씨를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 연꽃씨분말 및 우엉씨분말을 포함하는 제1 혼합분말을 준비하는 단계; 상기 제1 혼합분말, 1:1의 부피비로 혼합된 아세톤 및 클로로포름의 혼합 용매 및 1-에틸-3-메틸이미다졸리움 비스(트리플루오로메틸설포닐)이미드(EMIM TFSI, 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfylimide)를 혼합하여 유효성분을 포함하는 용액을 추출하는 단계; 및 추출된 용액을 감압농축 및 동결건조하는 단계;를 포함한다.First, the method for producing a cosmetic composition with whitening and antioxidant functions according to the present invention includes preparing a first natural extract containing lotus seed extract and burdock seed extract. The step of preparing the first natural extract includes washing lotus seeds and burdock seeds, cutting them into appropriate sizes, freeze-drying, and pulverizing them to prepare a first mixed powder containing lotus seed powder and burdock seed powder; The first mixed powder, a mixed solvent of acetone and chloroform mixed at a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMIM TFSI, 1-Ethyl- It includes extracting a solution containing the active ingredient by mixing 3-methylimidazolium bis (trifluoromethylsulfylimide), and concentrating and freeze-drying the extracted solution under reduced pressure.
상기 단계에서는 연꽃씨추출물 및 우엉씨추출물을 제조하되, 연꽃씨와 우엉씨를 함께 사용하여 추출물을 제조하며, 특히 씨앗으로부터 추출물을 추출함에 있어서 유효성분(폴리페놀, 플라보노이드 등) 함량이 높게 형성되도록 특정 용매와 첨가제로서 이온성액체를 도입하여 추출한다.In the above step, lotus seed extract and burdock seed extract are prepared, and the extract is prepared using lotus seed and burdock seed together. In particular, when extracting the extract from the seed, the content of active ingredients (polyphenols, flavonoids, etc.) is specified to be high. Extraction is performed by introducing ionic liquid as a solvent and additive.
먼저, 연꽃씨 및 우엉씨를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 연꽃씨분말 및 우엉씨분말을 포함하는 제1 혼합분말을 준비한다.First, the lotus seeds and burdock seeds are washed, cut into appropriate sizes, freeze-dried, and pulverized to prepare a first mixed powder containing lotus seed powder and burdock seed powder.
다음, 상기 제1 혼합분말, 1:1의 부피비로 혼합된 아세톤 및 클로로포름의 혼합 용매 및 1-에틸-3-메틸이미다졸리움 비스(트리플루오로메틸설포닐)이미드(EMIM TFSI, 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfylimide)를 혼합하여 유효성분을 포함하는 용액을 추출한다. Next, the first mixed powder, a mixed solvent of acetone and chloroform mixed at a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMIM TFSI, 1- A solution containing the active ingredient is extracted by mixing Ethyl-3-methylimidazolium bis (trifluoromethylsulfylimide).
상기 제1 혼합분말, 혼합 용매 및 EMIM TFSI은 1 : 20 : 0.5의 혼합비율(제1 혼합분말(g) : 혼합 용매(ml) : EMIm TFSI(g))로 혼합하는 것이 바람직하다.The first mixed powder, mixed solvent, and EMIM TFSI are preferably mixed at a mixing ratio of 1:20:0.5 (first mixed powder (g): mixed solvent (ml): EMIm TFSI (g)).
상기 추출은 20-40℃의 온도범위에서 12-24시간 동안 수행되는 것이 바람직하고, 30℃의 온도에서 12시간 동안 수행되는 것이 더욱 바람직하다. 또한, 상기 추출은 초음파공정으로 수행되는 것이 바람직하다.The extraction is preferably performed at a temperature range of 20-40°C for 12-24 hours, and more preferably at a temperature of 30°C for 12 hours. Additionally, the extraction is preferably performed using an ultrasonic process.
다음, 추출된 용액을 감압농축 및 동결건조한다. 구체적으로, 추출물을 여과지로 여과한 뒤 농축기로 55℃에서 감압농축 및 동결건조한다.Next, the extracted solution is concentrated under reduced pressure and freeze-dried. Specifically, the extract is filtered through filter paper, then concentrated under reduced pressure at 55°C in a concentrator and freeze-dried.
다음으로, 본 발명에 따른 미백 및 항산화기능을 가지는 화장품 조성물의 제조방법은 인동덩굴꽃추출물, 지모뿌리추출물 및 당개나리열매추출물을 포함하는 제2 천연추출물을 제조하는 단계를 포함한다. 상기 제2 천연추출물을 제조하는 단계는 인동덩굴꽃, 지모뿌리 및 당개나리열매를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 인동덩굴꽃분말, 지모뿌리분말 및 당개나리열매분말을 포함하는 제2 혼합분말을 준비하는 단계; 상기 제2 혼합분말 및 물을 혼합하고, 교반을 수행하며 95-105℃의 온도에서 10-14시간 동안 1차 열수추출을 수행하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 135-145℃의 온도에서 4-8시간 동안 2차 열수추출을 수행하는 단계; 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조하는 단계; 및 상기 제1 추출물 및 제2 추출물을 혼합하는 단계;를 포함한다.Next, the method for producing a cosmetic composition with whitening and antioxidant functions according to the present invention includes the step of preparing a second natural extract containing honeysuckle flower extract, syringa root extract, and forsythia fruit extract. The step of preparing the second natural extract includes washing honeysuckle flowers, roots and forsythia fruits, cutting them into appropriate sizes, freeze-drying and pulverizing them to produce honeysuckle flower powder, root powder and forsythia fruit powder. Preparing a second mixed powder; mixing the second mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105°C for 10-14 hours; After performing the first hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering through filter paper, mixing the remaining solids with water again, and performing the second hot water extraction at a temperature of 135-145°C for 4-8 hours; After secondary hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a second extract; and mixing the first extract and the second extract.
상기 단계에서는 인동덩굴꽃추출물, 지모뿌리추출물 및 당개나리열매추출물을 제조하되, 인동덩굴꽃, 지모뿌리 및 당개나리열매를 함께 사용하여 추출물을 제조하며, 특히 덩굴, 뿌리 또는 열매로부터 추출물을 추출함에 있어서 유효성분(폴리페놀, 플라보노이드 등) 함량이 높게 형성되도록 열수추출 공정을 도입하고, 특히 다단계 열수추출 공정을 도입하여 추출한다.In the above step, honeysuckle flower extract, root extract, and forsythia fruit extract are prepared, and the extract is prepared using honeysuckle flower, root root, and forsythia fruit together. In particular, extracts are extracted from vines, roots, or fruits. In order to form a high content of active ingredients (polyphenols, flavonoids, etc.), a hot water extraction process is introduced, and in particular, a multi-stage hot water extraction process is introduced to extract them.
먼저, 인동덩굴꽃, 지모뿌리 및 당개나리열매를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 인동덩굴꽃분말, 지모뿌리분말 및 당개나리열매분말을 포함하는 제2 혼합분말을 준비한다.First, wash the honeysuckle flowers, syringa root, and forsythia fruit, cut them into appropriate sizes, freeze-dry, and grind to prepare a second mixed powder containing honeysuckle flower powder, syringa root powder, and forsythia fruit powder. .
다음, 상기 제2 혼합분말 및 물을 혼합하고, 교반을 수행하며 95-105℃의 온도에서 10-14시간 동안 1차 열수추출을 수행한다.Next, the second mixed powder and water are mixed, stirred, and first hot water extraction is performed at a temperature of 95-105°C for 10-14 hours.
상기 1차 열수추출은 98-102℃의 온도에서 11-13시간 동안 수행하는 것이 바람직하고, 100℃의 온도에서 12시간 동안 수행하는 것이 가장 바람직하다. 상기 1차 열수추출은 100℃의 온도에서 12시간 동안 수행함으로써 2차 열수추출 시 추출성능을 극대화시켜 높은 유효성분 함량을 나타내는 추출물을 제조할 수 있다.The first hot water extraction is preferably performed at a temperature of 98-102°C for 11-13 hours, and most preferably at a temperature of 100°C for 12 hours. The first hot water extraction can be performed at a temperature of 100°C for 12 hours to maximize extraction performance during the second hot water extraction, thereby producing an extract with a high content of active ingredients.
다음, 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조한다.Next, after performing the first hot water extraction, it is filtered through filter paper and the filtrate is freeze-dried to prepare the first extract.
다음, 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 135-145℃의 온도에서 4-8시간 동안 2차 열수추출을 수행한다. 상기 2차 열수추출은 138-142℃의 온도에서 5-7시간 동안 수행하는 것이 바람직하고, 140℃의 온도에서 6시간 동안 수행하는 것이 가장 바람직하다. 상기 2차 열수추출은 140℃의 고온에서 6시간 동안 수행함으로써 1차 열수추출 후 얻어지는 고형물에서 유효성분을 더욱 높은 함량으로 추출할 수 있게 된다.Next, after performing the first hot water extraction, it is filtered through filter paper, the remaining solid is mixed with water again, and the second hot water extraction is performed at a temperature of 135-145°C for 4-8 hours. The secondary hot water extraction is preferably performed at a temperature of 138-142°C for 5-7 hours, and most preferably at a temperature of 140°C for 6 hours. The secondary hot water extraction is performed at a high temperature of 140°C for 6 hours, allowing the active ingredient to be extracted at a higher content from the solid obtained after the first hot water extraction.
다음, 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조한다. Next, after the second hot water extraction, it is filtered through filter paper and the filtrate is freeze-dried to prepare a second extract.
다음, 상기 제1 추출물 및 제2 추출물을 혼합한다.Next, the first extract and the second extract are mixed.
다음으로, 본 발명에 따른 미백 및 항산화기능을 가지는 화장품 조성물의 제조방법은 프로폴리스추출물, 병풀추출물 및 길경추출물을 포함하는 제3 천연추출물을 제조하는 단계를 포함한다. 상기 제3 천연추출물을 제조하는 단계는 프로폴리스, 병풀 및 길경을 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 프로폴리스분말, 병풀분말 및 길경분말을 포함하는 제3 혼합분말을 준비하는 단계; 상기 제3 혼합분말 및 에탄올을 혼합하여 유효성분을 포함하는 용액을 추출하는 단계; 및 추출된 용액을 감압농축 및 동결건조하는 단계;를 포함한다.Next, the method for producing a cosmetic composition with whitening and antioxidant functions according to the present invention includes the step of preparing a third natural extract including propolis extract, centella asiatica extract, and Gilge extract. The step of preparing the third natural extract includes washing propolis, centella asiatica, and apricot chinensis, cutting them into appropriate sizes, freeze-drying, and pulverizing them to prepare a third mixed powder containing propolis powder, centella asiatica powder, and apricot chinensis powder. step; extracting a solution containing the active ingredient by mixing the third mixed powder and ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure.
상기 단계에서는 프로폴리스추출물, 병풀추출물 및 길경추출물을 제조하되, 프로폴리스, 병풀 및 길경을 함께 사용하여 추출물을 제조하며, 프로폴리스와 병풀 및 길경을 함께 사용하여 추출물을 제조함으로써 프로폴리스추출물이 물에 대한 용해도가 낮은 문제를 병풀추출물 및 길경추출물이 천연계면활성제 역할을 하여 프로폴리스추출물의 용해도를 높여 화장품 조성물로 용이하게 혼합되도록 한다.In the above step, propolis extract, centella asiatica extract, and Gilgeum extract are prepared, and the extract is prepared using propolis, Centella asiatica, and Gilgeria together, and the extract is prepared by using propolis, Centella asiatica, and Gilgeria together, so that the propolis extract becomes water. To solve the problem of low solubility, Centella asiatica extract and Gilge extract act as natural surfactants to increase the solubility of propolis extract, allowing it to be easily mixed into cosmetic compositions.
먼저, 프로폴리스, 병풀 및 길경을 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 프로폴리스분말, 병풀분말 및 길경분말을 포함하는 제3 혼합분말을 준비한다.First, wash propolis, centella asiatica, and apricot root, cut into appropriate sizes, freeze-dry, and grind to prepare a third mixed powder containing propolis powder, centella asiatica powder, and apricot root.
다음, 상기 제3 혼합분말 및 에탄올을 혼합하여 유효성분을 포함하는 용액을 추출한다. 상기 추출은 20-40℃의 온도범위에서 12-24시간 동안 수행되는 것이 바람직하고, 20℃의 온도에서 12시간 동안 수행되는 것이 더욱 바람직하다. 또한, 상기 추출은 디핑공정으로 수행되는 것이 바람직하다.Next, the third mixed powder and ethanol are mixed to extract a solution containing the active ingredient. The extraction is preferably performed at a temperature range of 20-40°C for 12-24 hours, and more preferably at a temperature of 20°C for 12 hours. Additionally, the extraction is preferably performed by a dipping process.
다음, 추출된 용액을 감압농축 및 동결건조한다. 구체적으로, 추출물을 여과지로 여과한 뒤 농축기로 55℃에서 감압농축 및 동결건조한다.Next, the extracted solution is concentrated under reduced pressure and freeze-dried. Specifically, the extract is filtered through filter paper, then concentrated under reduced pressure at 55°C in a concentrator and freeze-dried.
다음으로, 본 발명에 따른 미백 및 항산화기능을 가지는 화장품 조성물의 제조방법은 상기 제1 천연추출물 28-32 중량부, 상기 제2 천연추출물 28-32 중량부 및 상기 제3 천연추출물 38-42 중량부를 혼합하여 천연복합추출물을 제조하는 단계를 포함한다.Next, the method for producing a cosmetic composition having whitening and antioxidant functions according to the present invention is to use 28-32 parts by weight of the first natural extract, 28-32 parts by weight of the second natural extract, and 38-42 parts by weight of the third natural extract. It includes the step of mixing the parts to produce a natural complex extract.
상기 단계에서는 전단계에서 제조되는 제1 천연추출물, 제2 천연추출물 및 제3 천연추출물을 모두 혼합하되, 상기 제1 천연추출물 28-32 중량부, 상기 제2 천연추출물 28-32 중량부 및 상기 제3 천연추출물 38-42 중량부를 혼합하여 천연복합추출물을 제조한다.In this step, the first natural extract, the second natural extract, and the third natural extract prepared in the previous step are all mixed, and 28-32 parts by weight of the first natural extract, 28-32 parts by weight of the second natural extract, and the third natural extract are mixed together. 3 Prepare a natural complex extract by mixing 38-42 parts by weight of natural extract.
더욱 바람직하게는, 상기 제1 천연추출물 29-31 중량부, 상기 제2 천연추출물 29-31 중량부 및 상기 제3 천연추출물 39-41 중량부를 혼합하여 천연복합추출물을 제조하고, 가장 바람직하게는, 상기 제1 천연추출물 30 중량부, 상기 제2 천연추출물 30 중량부 및 상기 제3 천연추출물 40 중량부를 혼합하여 천연복합추출물을 제조한다. 상기 제1-3 천연추출물을 상기 혼합비율로 혼합한 천연복합추출물을 화장품 조성물로 적용하여 우수한 항산화 활성 및 미백 기능을 나타낼 수 있다.More preferably, a natural complex extract is prepared by mixing 29-31 parts by weight of the first natural extract, 29-31 parts by weight of the second natural extract, and 39-41 parts by weight of the third natural extract, and most preferably, , 30 parts by weight of the first natural extract, 30 parts by weight of the second natural extract, and 40 parts by weight of the third natural extract are mixed to prepare a natural complex extract. A natural complex extract obtained by mixing the first to third natural extracts in the above mixing ratio can be applied as a cosmetic composition to exhibit excellent antioxidant activity and whitening function.
다음으로, 본 발명에 따른 미백 및 항산화기능을 가지는 화장품 조성물의 제조방법은 글루타치온 0.3-0.7 중량부, 토코페롤 0.5-2 중량부, 상기 천연복합추출물 0.5-3 중량부, 아스코빅애씨드 9-20 중량부, 에칠아스코빌에텔 1-2 중량부 및 트라넥사믹애씨드 1-3 중량부를 포함하는 조성물을 제조하는 단계를 포함한다.Next, the method for producing a cosmetic composition with whitening and antioxidant functions according to the present invention is 0.3-0.7 parts by weight of glutathione, 0.5-2 parts by weight of tocopherol, 0.5-3 parts by weight of the natural complex extract, and 9-20 parts by weight of ascorbic acid. parts, 1-2 parts by weight of ethyl ascorbyl ether, and 1-3 parts by weight of tranexamic acid.
글루타치온 0.3-0.7 중량부, 토코페롤 0.5-2 중량부, 상기 천연복합추출물 0.5-3 중량부, 아스코빅애씨드 9-20 중량부, 에칠아스코빌에텔 1-2 중량부 및 트라넥사믹애씨드 1-3 중량부를 포함하는 조성물을 제조하는 것이 더욱 바람직하다.Glutathione 0.3-0.7 parts by weight, tocopherol 0.5-2 parts by weight, 0.5-3 parts by weight of the above natural complex extract, ascorbic acid 9-20 parts by weight, ethyl ascorbyl ether 1-2 parts by weight, and tranexamic acid 1-3 It is more preferred to prepare a composition comprising parts by weight.
또한, 부틸렌글라이콜 1-6 중량부, 트로메타민 1-3 중량부, 프로판다이올 1-6 중량부, 암모늄아크릴로일다이메틸타우레이트/브이피코폴리머 0.1-1 중량부, 1,2-헥산다이올 1-3 중량부 및 나머지 잔부로 물을 포함하는 것이 바람직하고, 다이프로필렌글라이콜 1-6 중량부, 부틸렌글라이콜 1-6 중량부, 프로판다이올 1-6 중량부, 트로메타민 0.5-3 중량부, 잔탄검 0.1-0.5 중량부, 암모늄아크릴로일다이메틸타우레이트/브이피코폴리머 0.1-1 중량부, 폴리소르베이트80 0.1-2 중량부, 다이카프릴릴카보네이트 1-8 중량부, 다이메치콘,다이메티콘/비닐다이메티콘크로스폴리머 0.1-2 중량부, 1,2-헥산다이올 1-3 중량부, 에칠헥실글리세린 0.01-0.1 중량부 및 나머지 잔부로 물을 포함하는 것이 바람직하고, 부틸렌글라이콜 1-6 중량부, 프로판다이올 1-6 중량부, 소듐락테이트 1-5중량부, 1,2-헥산다이올 1-3 중량부, 에칠헥실글리세린 0.01-0.1 중량부, 다이프로필렌글라이콜 1-6중량부, 라우레스-23 1-5 중량부, 소듐하이알루로네이트 0.1-1 중량부 및 나머지 잔부로 물을 포함하는 것이 바람직하고, 다이프로필렌글라이콜 1-6 중량부, 부틸렌글라이콜 1-6 중량부, 프로판다이올 1-6 중량부, 트로메타민 0.5-3 중량부, 잔탄검 0.1-0.5 중량부, 암모늄아크릴로일다이메틸타우레이트/브이피코폴리머 0.1-1 중량부, 폴리소르베이트80 0.1-2 중량부, 세테아릴알코올 1-5 중량부, 다이카프릴릴카보네이트 1-8 중량부, 다이메치콘,다이메티콘/비닐다이메티콘크로스폴리머 0.1-2 중량부, 1,2-헥산다이올 1-3 중량부, 에칠헥실글리세린 0.01-0.1 중량부 및 나머지 잔부로 물을 포함하는 것이 바람직하다.Additionally, 1-6 parts by weight of butylene glycol, 1-3 parts by weight of tromethamine, 1-6 parts by weight of propanediol, 0.1-1 parts by weight of ammonium acryloyl dimethyl taurate/VP copolymer, 1, It is preferable to include 1-3 parts by weight of 2-hexanediol and the remainder containing water, 1-6 parts by weight of dipropylene glycol, 1-6 parts by weight of butylene glycol, and 1-6 parts by weight of propanediol. parts, tromethamine 0.5-3 parts by weight, xanthan gum 0.1-0.5 parts by weight, ammonium acryloyldimethyl taurate/VPicopolymer 0.1-1 parts by weight, polysorbate 80 0.1-2 parts by weight, dicapryl 1-8 parts by weight of reel carbonate, 0.1-2 parts by weight of dimethicone, dimethicone/vinyl dimethicone crosspolymer, 1-3 parts by weight of 1,2-hexanediol, 0.01-0.1 parts by weight of ethylhexylglycerin, and The remaining balance preferably contains water, 1-6 parts by weight of butylene glycol, 1-6 parts by weight of propanediol, 1-5 parts by weight of sodium lactate, and 1-3 parts by weight of 1,2-hexanediol. parts, 0.01-0.1 parts by weight of ethylhexylglycerin, 1-6 parts by weight of dipropylene glycol, 1-5 parts by weight of Laureth-23, 0.1-1 parts by weight of sodium hyaluronate, and the remainder containing water. It is preferable, 1-6 parts by weight of dipropylene glycol, 1-6 parts by weight of butylene glycol, 1-6 parts by weight of propanediol, 0.5-3 parts by weight of tromethamine, 0.1-0.5 parts by weight of xanthan gum. , 0.1-1 parts by weight of ammonium acryloyl dimethyl taurate/VP copolymer, 0.1-2 parts by weight of polysorbate 80, 1-5 parts by weight of cetearyl alcohol, 1-8 parts by weight of dicaprylyl carbonate, It contains 0.1-2 parts by weight of dimethicone, dimethicone/vinyl dimethicone crosspolymer, 1-3 parts by weight of 1,2-hexanediol, 0.01-0.1 parts by weight of ethylhexylglycerin, and the remainder is water. desirable.
이하, 본 발명을 하기의 실시예에 의해 보다 상세하게 설명한다.Hereinafter, the present invention will be explained in more detail by the following examples.
단, 하기 실시예는 본 발명의 내용을 예시하는 것일 뿐 발명의 범위가 실시예 및 실험예에 의해 한정되는 것은 아니다.However, the following examples only illustrate the content of the present invention and the scope of the invention is not limited by the examples and experimental examples.
<제조예 1> 제1 천연추출물의 제조<Preparation Example 1> Preparation of the first natural extract
연꽃씨와 우엉씨를 준비하여 세척하고, 적절한 크기로 잘라 동결건조 시킨 후, 믹서기로 갈아 분쇄시켜 혼합물말을 제조한다.Prepare lotus seeds and burdock seeds, wash them, cut them into appropriate sizes, freeze-dry them, grind them in a blender, and grind them to prepare the mixture.
상기 혼합분말 10 g을 아세톤 및 클로로포름이 1:1의 부피비로 혼합된 혼합용매 200 ml와 혼합하고, 여기에 1-에틸-3-메틸이미다졸리움 비스(트리플루오로메틸설포닐)이미드(EMIM TFSI, 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfylimide)를 5 g 첨가하고, 초음파공정(ultra sonic)을 수행하여 30℃의 온도에서 12시간 동안 추출한 후, 여과지로 여과한 뒤 농축기로 55℃에서 감압농축 및 동결건조하여 제1 천연추출물을 얻었다.10 g of the mixed powder was mixed with 200 ml of a mixed solvent containing acetone and chloroform in a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ( Add 5 g of EMIM TFSI, 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfylimide), perform ultrasonic extraction at 30°C for 12 hours, filter through filter paper, and extract in a concentrator at 55°C. The first natural extract was obtained by concentration under reduced pressure and freeze-drying.
<제조예 2> 제2 천연추출물의 제조<Preparation Example 2> Preparation of the second natural extract
인동덩굴꽃, 지모뿌리추출물 및 당개나리열매를 준비하여 세척하고, 적절한 크기로 잘라 동결건조 시킨 후, 믹서기로 갈아 분쇄시켜 혼합물말을 제조한다.Prepare and wash honeysuckle flowers, syringa root extract, and forsythia fruit, cut them into appropriate sizes, freeze-dry them, grind them in a blender, and grind them to prepare the mixture.
상기 혼합분말 10 g을 물 100 ml와 혼합하고, 교반하면서 100℃의 온도로 가열하여 12시간 동안 1차 열수추출을 수행하였다. 이후, 여과지로 여과하고, 그 여액을 동결건조하여 제1 추출물을 제조하였다. 여과된 고형물을 다시 물 98 ml와 혼합하고, 교반 없이 140℃의 온도로 가열하여 6시간 동안 2차 열수추출을 수행하였다. 이후, 여과지로 여과하고, 그 여액을 동결건조하여 제2 추출물을 제조하였다. 최종적으로 제1 추출물 및 제2 추출물을 혼합하여 제2 천연추출물을 얻었다.10 g of the mixed powder was mixed with 100 ml of water, heated to a temperature of 100°C while stirring, and primary hot water extraction was performed for 12 hours. Afterwards, it was filtered through filter paper, and the filtrate was freeze-dried to prepare the first extract. The filtered solid was mixed again with 98 ml of water, heated to a temperature of 140°C without stirring, and secondary hot water extraction was performed for 6 hours. Afterwards, it was filtered through filter paper, and the filtrate was freeze-dried to prepare a second extract. Finally, the first extract and the second extract were mixed to obtain a second natural extract.
<제조예 3> 제3 천연추출물의 제조<Preparation Example 3> Preparation of third natural extract
프로폴리스, 병풀 및 길경을 준비하여 세척하고, 적절한 크기로 잘라 동결건조 시킨 후, 믹서기로 갈아 분쇄시켜 혼합물말을 제조한다.Prepare and wash propolis, centella asiatica, and apricot root, cut them into appropriate sizes, freeze-dry them, and grind them in a blender to prepare the mixture.
상기 혼합분말 10 g을 에탄올 80 ml와 혼합하고, 디핑공정을 수행하여 20℃의 온도에서 12시간 동안 추출한 후, 여과지로 여과한 뒤 농축기로 55℃에서 감압농축 및 동결건조하여 제3 천연추출물을 얻었다.10 g of the mixed powder was mixed with 80 ml of ethanol, subjected to a dipping process and extracted at a temperature of 20°C for 12 hours, filtered through filter paper, concentrated under reduced pressure at 55°C using a concentrator, and freeze-dried to produce the third natural extract. got it
<제조예 4> 천연복합추출물의 제조<Preparation Example 4> Preparation of natural complex extract
상기 제조예 1에서 제조된 제1 천연추출물 30 중량부, 상기 제조예 2에서 제조된 제2 천연추출물 30 중량부 및 상기 제조예 3에서 제조된 제3 천연추출물 40 중량부를 혼합하여 천연복합추출물을 제조하였다.A natural complex extract was prepared by mixing 30 parts by weight of the first natural extract prepared in Preparation Example 1, 30 parts by weight of the second natural extract prepared in Preparation Example 2, and 40 parts by weight of the third natural extract prepared in Preparation Example 3. Manufactured.
<제조예 5> 스킨 제형의 화장품 조성물 제조<Preparation Example 5> Preparation of skin formulation cosmetic composition
스킨 제형 제조는 수상파트 글루타치온, 아스코빅애씨드, 에칠아스코빌에텔, 트라넥사믹애씨드, 부틸렌글라이콜, 트로메타민, 프로판다이올과 가용화 파트 토코페롤, 라우레스-23을 50℃의 온도에서 녹인 후 수상파트와 가용화 파트를 섞는다. 점증제 파트를 75-80℃의 온도로 가온한 정제수에 암모늄아크릴로일다이메틸타우레이트/브이피코폴리머를 용해시켜 투입한 후 첨가물 파트인 천연복합추출물, 1,2-헥산다이올을 넣은 후 제조하였다. 각 성분의 함량은 하기 표 1에 나타내었다.The skin formulation is manufactured by dissolving the aqueous part glutathione, ascorbic acid, ethyl ascorbyl ether, tranexamic acid, butylene glycol, tromethamine, and propanediol and the solubilized part tocopherol and laureth-23 at a temperature of 50℃. Then mix the aqueous part and the solubilized part. Add the thickener part by dissolving ammonium acryloyldimethyl taurate/VP copolymer in purified water heated to a temperature of 75-80℃, then add the additive parts, natural complex extract and 1,2-hexanediol. Manufactured. The content of each component is shown in Table 1 below.
성분 명 Ingredient name 비교예 1Comparative Example 1 실시예 1 Example 1 실시예 2Example 2 비교예 2Comparative Example 2 실시예 3Example 3
정제수Purified water Total 100Total 100 Total 100Total 100 Total 100Total 100 Total 100Total 100 Total 100Total 100
글루타치온glutathione 0.00.0 0.30.3 0.60.6 0.00.0 0.40.4
토코페롤tocopherol 0.00.0 0.50.5 0.80.8 0.00.0 1.01.0
천연복합추출물Natural complex extract 0.00.0 1.01.0 0.50.5 0.30.3 2.02.0
아스코빅애씨드 Ascorbic Acid 1010 1010 1010 1010 1010
에칠아스코빌에텔Ethyl Ascorbyl Ether 0.00.0 1.01.0 1.51.5 0.00.0 2.02.0
트라넥사믹애씨드Tranexamic Acid 0.00.0 2.52.5 2.02.0 0.00.0 2.02.0
부틸렌글라이콜Butylene glycol 6.06.0 6.06.0 6.06.0 6.06.0 6.06.0
트로메타민Tromethamine 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5
프로판다이올Propanediol 6.06.0 6.06.0 6.06.0 6.06.0 6.06.0
1,2-헥산다이올1,2-hexanediol 2.02.0 2.02.0 2.02.0 2.02.0 2.02.0
라우레스-23Laureth-23 2.02.0 2.02.0 2.02.0 2.02.0 2.02.0
암모늄아크릴로일다이메틸타우레이트/브이피코폴리머Ammonium Acryloyl Dimethyl Taurate/VPicopolymer 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2
<제조예 6> 로션 제형의 화장품 조성물 제조<Preparation Example 6> Preparation of lotion-type cosmetic composition
로션 제형 제조시 수상파트인 글루타치온, 아스코빅애씨드, 에칠아스코빌에텔, 트라넥사믹애씨드, 다이프로필렌글라이콜, 부틸렌글라이콜, 프로판다이올, 트로메타민과 유상파트인 토코페롤, 폴리소르베이트80, 다이카프릴릴카보네이트, 다이메치콘,다이메티콘/비닐다이메티콘크로스폴리머를 각각 측량하여 따로 달고 75-80℃의 온도로 가온하여 수상파트와 유상파트를 혼합 교반(75-80℃, Homo mix 3000 rpm, 5분)한다. 점증제 파트인 잔탄검, 암모늄아크릴로일다이메틸타우레이트/브이피코폴리머를 75-80℃의 온도인 아지믹서로 용해시킨 후 투입 혼합교반(75-80℃, Homo mix 3000 rpm, 5분)한다. 50-60℃의 온도로 냉각 후 첨가물 파트 천연복합추출물, 1,2-헥산다이올, 에칠헥실글리세린을 넣어 혼합 교반(50-60℃, Homo mix 2000 rpm, 5분) 후 냉각(30-35℃) 탈포한다. 각 성분의 함량은 하기 표 2에 나타내었다.When manufacturing lotion formulations, the aqueous parts such as glutathione, ascorbic acid, ethyl ascorbyl ether, tranexamic acid, dipropylene glycol, butylene glycol, propanediol, tromethamine and the oil parts such as tocopherol and polysorbate are used. 80, measure dicaprylyl carbonate, dimethicone, and dimethicone/vinyl dimethicone crosspolymer separately, heat to a temperature of 75-80℃, mix and stir the aqueous phase and oil phase (75-80℃) , Homo mix 3000 rpm, 5 minutes). The thickener parts, xanthan gum and ammonium acryloyl dimethyl taurate/VP copolymer, are dissolved in an azimixer at a temperature of 75-80℃, then added and mixed and stirred (75-80℃, Homo mix 3000 rpm, 5 minutes). do. After cooling to a temperature of 50-60℃, add the additive part natural complex extract, 1,2-hexanediol, and ethylhexylglycerin, mix and stir (50-60℃, Homo mix 2000 rpm, 5 minutes), and cool (30-35℃). ℃) degassing. The content of each component is shown in Table 2 below.
성분 명 Ingredient name 비교예 3Comparative Example 3 실시예 4Example 4 실시예 5Example 5 비교예4Comparative Example 4 실시예 6Example 6
정제수Purified water Total 100Total 100 Total 100Total 100 Total 100Total 100 Total 100Total 100 Total 100Total 100
글루타치온glutathione 0.00.0 0.30.3 0.40.4 0.00.0 0.50.5
토코페롤tocopherol 0.00.0 0.80.8 1.21.2 0.00.0 1.51.5
천연복합추출물Natural complex extract 0.00.0 0.80.8 0.50.5 0.20.2 2.02.0
아스코빅애씨드 Ascorbic Acid 1212 1212 1212 1212 1212
에칠아스코빌에텔Ethyl Ascorbyl Ether 0.00.0 1.01.0 1.51.5 0.00.0 2.02.0
트라넥사믹애씨드Tranexamic Acid 0.00.0 0.30.3 0.30.3 0.00.0 0.30.3
다이프로필렌글라이콜Dipropylene glycol 6.06.0 6.06.0 6.06.0 6.06.0 6.06.0
부틸렌글라이콜Butylene glycol 6.06.0 6.06.0 6.06.0 6.06.0 6.06.0
프로판다이올Propanediol 6.06.0 6.06.0 6.06.0 6.06.0 6.06.0
트로메타민Tromethamine 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0
잔탄검xanthan gum 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2
암모늄아크릴로일다이메틸타우레이트/브이피코폴리머Ammonium Acryloyl Dimethyl Taurate/VPicopolymer 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2
폴리소르베이트80 Polysorbate 80 0.60.6 0.60.6 0.60.6 0.60.6 0.60.6
다이카프릴릴카보네이트dicaprylyl carbonate 4.04.0 4.04.0 4.04.0 4.04.0 4.04.0
다이메티콘,다이메티콘/비닐다이메티콘크로스폴리머Dimethicone, dimethicone/vinyl dimethicone crosspolymer 0.60.6 0.60.6 0.60.6 0.60.6 0.60.6
1,2-헥산다이올1,2-hexanediol 2.02.0 2.02.0 2.02.0 2.02.0 2.02.0
에칠헥실글리세린Ethylhexylglycerin 0.050.05 0.050.05 0.050.05 0.050.05 0.050.05
<제조예 7> 세럼 제형의 화장품 조성물 제조<Preparation Example 7> Preparation of serum-type cosmetic composition
세럼 제형 제조시 수상파트 글루타치온, 아스코빅애씨드, 에칠아스코빌에텔, 트라넥사믹애씨드, 부틸렌글라이콜, 프로판다이올, 다이프로필렌글라이콜을 50-60℃의 온도로 가온하여 아지믹서로 용해시킨 후 가용화 파트 토코페롤, 라우레스-23을 혼합한 후 수상파트에 천천히 투입 후 아지믹서로 용해 시켜준다. 점증제 파트는 75-80℃ 온도의 정제수로 소듐하이알루로네이트를 용해 후 투입 교반한다. 첨가물 파트 천연복합추출물, 소듐락테이트, 1,2-헥산다이올, 에칠헥실글리세린을 넣은 후 제조하였다. 각 성분의 함량은 하기 표 3에 나타내었다.When preparing a serum formulation, heat the aqueous parts glutathione, ascorbic acid, ethyl ascorbyl ether, tranexamic acid, butylene glycol, propanediol, and dipropylene glycol to a temperature of 50-60℃ and dissolve them with an azimixer. After mixing the solubilization part tocopherol and laureth-23, slowly add it to the aqueous part and dissolve it with an azimixer. For the thickener part, sodium hyaluronate is dissolved in purified water at a temperature of 75-80℃, then added and stirred. It was prepared by adding natural complex extract, sodium lactate, 1,2-hexanediol, and ethylhexylglycerin as additive parts. The content of each component is shown in Table 3 below.
성분 명 Ingredient name 비교예 5Comparative Example 5 실시예 7Example 7 실시예 8Example 8 비교예 6Comparative Example 6 실시예 9Example 9
정제수Purified water Total 100Total 100 Total 100Total 100 Total 100Total 100 Total 100Total 100 Total 100Total 100
글루타치온glutathione 0.00.0 0.60.6 0.40.4 0.00.0 0.70.7
토코페롤tocopherol 0.00.0 1.01.0 1.01.0 0.00.0 0.50.5
천연복합추출물Natural complex extract 0.00.0 1.01.0 1.01.0 0.150.15 1.01.0
아스코빅애씨드Ascorbic Acid 1515 1515 1515 1515 1515
에칠아스코빌에텔Ethyl Ascorbyl Ether 0.00.0 1.01.0 1.51.5 0.00.0 1.31.3
트라넥사믹애씨드Tranexamic Acid 0.00.0 1.01.0 1.51.5 0.00.0 2.02.0
부틸렌글라이콜Butylene glycol 5.05.0 5.05.0 5.05.0 5.05.0 5.05.0
프로판다이올Propanediol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0
소듐락테이트Sodium Lactate 1.61.6 1.61.6 1.61.6 1.61.6 1.61.6
1,2-헥산다이올1,2-hexanediol 2.02.0 2.02.0 2.02.0 2.02.0 2.02.0
에칠헥실글리세린Ethylhexylglycerin 0.050.05 0.050.05 0.050.05 0.050.05 0.050.05
다이프로필렌글라이콜Dipropylene glycol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0
라우레스-23Laureth-23 2.02.0 2.02.0 2.02.0 2.02.0 2.02.0
소듐하이알루로네이트Sodium Hyaluronate 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1
<제조예 8> 크림 제형의 화장품 조성물 제조<Preparation Example 8> Preparation of cream-type cosmetic composition
크림 제형 제조 시 수상파트 글루타치온,아스코빅애씨드, 에칠아스코빌에텔, 트라넥사믹애씨드, 다이프로필렌글라이콜, 부틸렌글라이콜, 프로판다이올, 트로메타민, 유상파트 토코페롤, 폴리소르베이트80, 세테아릴알코올, 다이카프릴릴카보네이트, 다이메치콘,다이메티콘/비닐다이메티콘크로스폴리머를 각각 측량하여 따로 달고 75-80℃의 온도로 가온하여 수상파트와 유상파트를 혼합 교반(75-80℃, Homo mix 3000 rpm, 5분)한다. 점증제 파트 잔탄검, 암모늄아크릴로일다이메틸타우레이트/브이피코폴리머, 75-80℃ 온도의 아지믹서로 용해 시킨 후 투입 혼합교반(75-80℃, Homo mix 3000 rpm, 5분)한다. 50-60℃의 온도로 냉각 후 첨가물 파트 천연복합추출물, 1,2-헥산다이올, 에칠헥실글리세린을 전부 넣어 혼합 교반(50-60℃, Homo mix 2000 rpm, 5분) 후 냉각(30-35℃) 탈포한다. 각 성분의 함량은 하기 표 4에 나타내었다.When manufacturing cream formulations, the water part glutathione, ascorbic acid, ethyl ascorbyl ether, tranexamic acid, dipropylene glycol, butylene glycol, propanediol, tromethamine, the oil part tocopherol, polysorbate 80, Cetearyl alcohol, dicaprylyl carbonate, dimethicone, dimethicone/vinyl dimethicone crosspolymer were weighed separately, heated to a temperature of 75-80°C, mixed and stirred (75°C). -80℃, Homo mix 3000 rpm, 5 minutes). The thickener part, xanthan gum, ammonium acryloyl dimethyl taurate/VP copolymer, is dissolved in an Azi mixer at a temperature of 75-80℃, then added and mixed and stirred (75-80℃, Homo mix 3000 rpm, 5 minutes). After cooling to a temperature of 50-60℃, add all the additive parts, natural complex extract, 1,2-hexanediol, and ethylhexylglycerin, mix and stir (50-60℃, Homo mix 2000 rpm, 5 minutes), and cool (30-60℃). 35℃) Degassing. The content of each component is shown in Table 4 below.
성분 명 Ingredient name 비교예 7Comparative Example 7 실시예 10Example 10 실시예 11Example 11 비교예 8Comparative Example 8 실시예 12Example 12
정제수Purified water Total 100Total 100 Total 100Total 100 Total 100Total 100 Total 100Total 100 Total 100Total 100
글루타치온glutathione 0.00.0 0.60.6 0.40.4 0.00.0 0.70.7
토코페롤tocopherol 0.00.0 1.81.8 1.51.5 0.00.0 2.02.0
천연복합추출물Natural complex extract 0.00.0 22 1.01.0 0.250.25 1.51.5
아스코빅애씨드Ascorbic Acid 1717 1717 1717 1717 1717
에칠아스코빌에텔Ethyl Ascorbyl Ether 0.00.0 2.02.0 1.01.0 0.00.0 2.02.0
트라넥사믹애씨드Tranexamic Acid 0.00.0 1.01.0 1.51.5 0.00.0 2.02.0
다이프로필렌글라이콜Dipropylene glycol 5.05.0 5.05.0 5.05.0 5.05.0 5.05.0
부틸렌글라이콜Butylene glycol 5.05.0 5.05.0 5.05.0 5.05.0 5.05.0
프로판다이올Propanediol 5.05.0 5.05.0 5.05.0 5.05.0 5.05.0
트로메타민Tromethamine 2.02.0 2.02.0 2.02.0 2.02.0 2.02.0
잔탄검xanthan gum 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2
암모늄아크릴로일다이메틸타우레이트/브이피코폴리머Ammonium Acryloyl Dimethyl Taurate/VPicopolymer 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2
폴리소르베이트80 Polysorbate 80 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5
세테아릴알코올Cetearyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0
다이카프릴릴카보네이트dicaprylyl carbonate 5.05.0 5.05.0 5.05.0 5.05.0 5.05.0
다이메티콘, 다이메티콘/비닐다이메티콘크로스폴리머Dimethicone, dimethicone/vinyl dimethicone crosspolymer 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5
1,2-헥산다이올1,2-hexanediol 2.02.0 2.02.0 2.02.0 2.02.0 2.02.0
에칠헥실글리세린Ethylhexylglycerin 0.050.05 0.050.05 0.050.05 0.050.05 0.050.05
<실험방법 1> 항산화 활성 분석<Experimental Method 1> Antioxidant activity analysis
전자공여능 측정 방법Electron donating ability measurement method
DPPH(2,2-diphenyl-1-picrylhydrazyl) assay는 빠르고 간단하게 이용할 수 있는 측정법으로 식물이나 식품 추출물 또는 단일 화합물의 항산화능을 측정하기 위해 널리 사용되는 방법이다. DPPH는 짙은 보라색을 나타내는 organic nitrogen radical로 항산화 활성이 있는 물질과 만나면 매우 빠른 속도로 hydrogen radical의 전자를 받아들이면서 환원되어 안정한 화합물인 2,2-diphenyl-1-picryl hydrazine으로 비가역적으로 전환되며 짙은 보라색이 엷어지는 특징을 가진다. 색깔이 엷어지는 정도는 추출물이나 화합물의 DPPH radical 소거능이 큰 것을 의미한다.DPPH (2,2-diphenyl-1-picrylhydrazyl) assay is a quick and simple measurement method that is widely used to measure the antioxidant capacity of plants, food extracts, or single compounds. DPPH is an organic nitrogen radical that is dark purple in color. When it comes into contact with a substance with antioxidant activity, it is reduced by accepting electrons from the hydrogen radical at a very rapid rate and is irreversibly converted to the stable compound 2,2-diphenyl-1-picryl hydrazine, forming a dark substance. It has the characteristic of fading purple color. The degree to which the color becomes lighter means that the DPPH radical scavenging ability of the extract or compound is high.
DPPH radical과 antioxidants의 반응 과정을 간단히 나타내면 아래와 같다.The reaction process between DPPH radical and antioxidants is briefly expressed as follows.
Figure PCTKR2022012106-appb-img-000001
Figure PCTKR2022012106-appb-img-000001
DPPH assay 측정 결과는 시료 첨가구의 흡광도와 무처리구의 흡광도의 차이를 이용하여 백분율로 나타내며 아래의 식으로 구할 수 있다.The DPPH assay measurement result is expressed as a percentage using the difference between the absorbance of the sample added group and the absorbance of the untreated group and can be obtained using the formula below.
Figure PCTKR2022012106-appb-img-000002
Figure PCTKR2022012106-appb-img-000002
여기서, A는 시료 첨가구의 흡광도, B는 무처리구의 흡광도임.Here, A is the absorbance of the sample addition section, and B is the absorbance of the untreated section.
전자공여능 실험에 이용한 DPPH(2,2-diphenyl-1-picryl hydrazyl) 용액은 Methanol에 녹여 0.25mM로 만들어 사용하였다. 96well plate에 90uL 0.25mM DPPH (1,1-diphenyl-2-picryl-hydrazyl)용액과 100uL MeOH을 가한 후 농도별 추출물을 10uL씩 가하고 차광상태에서 30분간 반응시켰다. 반응 후 흡광도기를 이용하여 517nm에서 흡광도를 측정하였다. 양성대조군으로 Ascorbic acid, Kojic acid 등을 사용한다.The DPPH (2,2-diphenyl-1-picryl hydrazyl) solution used in the electron donating ability experiment was dissolved in methanol to make 0.25mM. After adding 90uL 0.25mM DPPH (1,1-diphenyl-2-picryl-hydrazyl) solution and 100uL MeOH to a 96 well plate, 10uL of each concentration of extract was added and reacted for 30 minutes under light blocking conditions. After reaction, the absorbance was measured at 517 nm using an absorbance meter. Ascorbic acid, Kojic acid, etc. are used as positive controls.
<실험방법 2> 미백 기능 분석<Experiment Method 2> Whitening function analysis
Tyrosinase 저해 활성 측정Tyrosinase inhibitory activity measurement
색소 침착은 외부에서 자외선, 염증 등의 여러 자극에 의해 야기되는 것으로 알려져있다. 이는 표피 기저층에 존재하는 melanocyte에서 일어나며 자외선에 의한 색소침착의 메커니즘은 tyrosinase 발현 제어 인자가 주 역할을 한다.It is known that pigmentation is caused by various external stimuli such as ultraviolet rays and inflammation. This occurs in melanocytes present in the basal layer of the epidermis, and the mechanism of pigmentation caused by ultraviolet rays is mainly played by the tyrosinase expression control factor.
Tyrosinase 저해 활성 측정 실험은 색소 침착 과정에서 tyrosinase가 DOPA를 DOPA quinone으로 산화하는 DOPA oxidase 활성을 가지고 있는 것을 이용한 실험이다. Tyrosinase와 L-DOPA의 반응으로 생긴 DOPA chrome의 생성량을 비교하여 시료의 효소활성 억제능을 측정한다.Tyrosinase inhibitory activity measurement experiment uses the fact that tyrosinase has DOPA oxidase activity, which oxidizes DOPA to DOPA quinone during the pigmentation process. The enzyme activity inhibition ability of the sample is measured by comparing the amount of DOPA chrome produced by the reaction between tyrosinase and L-DOPA.
Tyrosinase저해 활성실험의 Sodium phosphate buffer는 0.175M, pH6.8이 되도록 제조하여 사용하였다. L-DOPA (3,4-dihydroxy-L-phenyl-alanine)와 Tyrosinase from mushroom는 각각 10mM와 1000U/mL이 되도록 Sodium phosphate buffer에 녹여 사용하였다.Sodium phosphate buffer for the tyrosinase inhibitory activity test was prepared and used at 0.175M, pH 6.8. L-DOPA (3,4-dihydroxy-L-phenyl-alanine) and Tyrosinase from mushroom were dissolved in sodium phosphate buffer to 10mM and 1000U/mL, respectively.
96well에 시료 20ul, 0.175M Sodium phospate buffer(pH6.8) 100ul, L-DOPA (3,4-dihydroxy-L-phenyl-alanine) 40ul와 Tyrosinase 1000U/mL 40ul을 혼합하여 37℃ incubator (Sejong, SJ-8085F)에서 2분간 반응시킨 뒤 492nm의 파장에서 흡광도를 측정했다.Mix 20ul of sample, 100ul of 0.175M sodium phospate buffer (pH6.8), 40ul of L-DOPA (3,4-dihydroxy-L-phenyl-alanine) and 40ul of Tyrosinase 1000U/mL in 96 well and incubate at 37℃ (Sejong, SJ). After reacting for 2 minutes at -8085F), the absorbance was measured at a wavelength of 492nm.
<실험방법 3> 안정성 평가 실험<Experiment Method 3> Stability evaluation experiment
화장품 내용물 안정성 평가 확인 실험은 4℃, 40℃, 25℃ 3가지 온도에서 장기 보존했을 때 성상, 향취, 점도, pH 등 변화 등 평가하며 1일, 7일, 3주, 5주, 8주 기간동안 확인하는 실험을 실시하여 성상(색상변화) 및 향취 등을 확인한다.The stability evaluation and confirmation experiment of cosmetic contents evaluates changes in appearance, odor, viscosity, pH, etc. when stored for a long period of time at three temperatures of 4℃, 40℃, and 25℃, and is conducted over a period of 1 day, 7 days, 3 weeks, 5 weeks, and 8 weeks. Conduct an experiment to check the appearance (color change) and odor.
<실험결과 1> 안정도 평가 실험 결과<Experiment Result 1> Stability evaluation experiment results
[규칙 제91조에 의한 정정 25.08.2022]
1) 스킨제형에 대한 안정도 평가 실험 결과를 표 5에 나타내었다(도 12 참조).[Correction 25.08.2022 pursuant to Rule 91]
1) The results of the stability evaluation experiment for the skin formulation are shown in Table 5 (see Figure 12).
[규칙 제91조에 의한 정정 25.08.2022][Correction 25.08.2022 pursuant to Rule 91]
[규칙 제91조에 의한 정정 25.08.2022]
2) 로션제형에 대한 안정도 평가 실험 결과를 표 6에 나타내었다(도 13 참조).[Correction 25.08.2022 pursuant to Rule 91]
2) The results of the stability evaluation experiment for the lotion formulation are shown in Table 6 (see Figure 13).
[규칙 제91조에 의한 정정 25.08.2022][Correction 25.08.2022 pursuant to Rule 91]
[규칙 제91조에 의한 정정 25.08.2022]
3) 세럼제형에 대한 안정도 평가 실험 결과를 표 7에 나타내었다(도 14 참조).[Correction 25.08.2022 pursuant to Rule 91]
3) The results of the stability evaluation experiment for the serum formulation are shown in Table 7 (see Figure 14).
[규칙 제91조에 의한 정정 25.08.2022][Correction 25.08.2022 pursuant to Rule 91]
[규칙 제91조에 의한 정정 25.08.2022]
4) 크림제형에 대한 안정도 평가 실험 결과를 표 8에 나타내었다(도 15 참조).[Correction 25.08.2022 pursuant to Rule 91]
4) The results of the stability evaluation experiment for the cream formulation are shown in Table 8 (see Figure 15).
[규칙 제91조에 의한 정정 25.08.2022][Correction 25.08.2022 pursuant to Rule 91]
<실험결과 2> 전자공여능 측정 시험 결과<Experiment Result 2> Electron donation ability measurement test results
전자공여능 측정 시험 결과를 도 4 내지 도 7에 나타내었다.The results of the electron donating ability measurement test are shown in Figures 4 to 7.
도 4는 스킨제형의 실시예 및 비교예의 전자공여능 측정 시험 결과이고, 도 5는 로션제형의 실시예 및 비교예의 전자공여능 측정 시험 결과이고, 도 6은 세럼제형의 실시예 및 비교예의 전자공여능 측정 시험 결과이고, 도 7은 크림제형의 실시예 및 비교예의 전자공여능 측정 시험 결과이다.Figure 4 shows the electron donating ability measurement test results of Examples and Comparative Examples of skin formulations, Figure 5 shows the electron donating ability measurement test results of Examples and Comparative Examples of lotion formulations, and Figure 6 shows the electron donating ability measurement test results of Examples and Comparative Examples of serum formulations. These are the test results, and Figure 7 shows the electron donating ability measurement test results of the cream formulation examples and comparative examples.
<실험결과 3> Tyrosinase 저해 활성 측정 시험 결과<Experiment Result 3> Tyrosinase inhibitory activity measurement test results
Tyrosinase 저해 활성 측정 시험 결과를 도 8 내지 도 11에 나타내었다.The results of the tyrosinase inhibitory activity measurement test are shown in Figures 8 to 11.
도 8은 스킨제형의 실시예 및 비교예의 Tyrosinase 저해 활성 측정 시험 결과이고, 도 9는 로션제형의 실시예 및 비교예의 Tyrosinase 저해 활성 측정 시험 결과이고, 도 10은 세럼제형의 실시예 및 비교예의 Tyrosinase 저해 활성 측정 시험 결과이고, 도 11은 크림제형의 실시예 및 비교예의 Tyrosinase 저해 활성 측정 시험 결과이다.Figure 8 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of skin formulations, Figure 9 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of lotion formulations, and Figure 10 shows the results of a test for measuring Tyrosinase inhibitory activity of Examples and Comparative Examples of serum formulations. These are the results of the inhibitory activity measurement test, and Figure 11 shows the results of the Tyrosinase inhibitory activity measurement test of the examples and comparative examples of cream formulations.

Claims (4)

  1. 글루타치온, 토코페롤, 천연복합추출물, 아스코빅애씨드, 에칠아스코빌에텔 및 트라넥사믹애씨드를 포함하는 미백 및 항산화기능을 가지는 화장품 조성물.A cosmetic composition with whitening and antioxidant functions containing glutathione, tocopherol, natural complex extract, ascorbic acid, ethyl ascorbyl ether, and tranexamic acid.
  2. 제1항에 있어서,According to paragraph 1,
    상기 화장품 조성물은 글루타치온 0.3-0.7 중량부, 토코페롤 0.5-2 중량부, 상기 천연복합추출물 0.5-3 중량부, 아스코빅애씨드 9-20 중량부, 에칠아스코빌에텔 1-2 중량부 및 트라넥사믹애씨드 1-3 중량부를 포함하는 것을 특징으로 하는 미백 및 항산화기능을 가지는 화장품 조성물.The cosmetic composition contains 0.3-0.7 parts by weight of glutathione, 0.5-2 parts by weight of tocopherol, 0.5-3 parts by weight of the natural complex extract, 9-20 parts by weight of ascorbic acid, 1-2 parts by weight of ethyl ascorbyl ether, and tranexamic. A cosmetic composition with whitening and antioxidant functions, comprising 1-3 parts by weight of acid.
  3. 제1항 또는 제2항에 있어서,According to claim 1 or 2,
    상기 천연복합추출물은 연꽃씨추출물, 인동덩굴꽃추출물, 우엉씨추출물, 지모뿌리추출물, 당개나리열매추출물, 프로폴리스추출물, 병풀추출물 및 길경추출물을 포함하는 것을 특징으로 하는 미백 및 항산화기능을 가지는 화장품 조성물.The natural complex extract is a cosmetic product with whitening and antioxidant functions, comprising lotus seed extract, honeysuckle flower extract, burdock seed extract, Gimo root extract, Forsythia fruit extract, propolis extract, centella asiatica extract, and Gilge extract. Composition.
  4. 연꽃씨 및 우엉씨를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 연꽃씨분말 및 우엉씨분말을 포함하는 제1 혼합분말을 준비하는 단계; 상기 제1 혼합분말, 1:1의 부피비로 혼합된 아세톤 및 클로로포름의 혼합 용매 및 1-에틸-3-메틸이미다졸리움 비스(트리플루오로메틸설포닐)이미드(EMIM TFSI, 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfylimide)를 혼합하여 유효성분을 포함하는 용액을 추출하는 단계; 및 추출된 용액을 감압농축 및 동결건조하는 단계;를 포함하는 연꽃씨추출물 및 우엉씨추출물을 포함하는 제1 천연추출물을 제조하는 단계;Washing lotus seeds and burdock seeds, cutting them into appropriate sizes, freeze-drying, and pulverizing them to prepare a first mixed powder containing lotus seed powder and burdock seed powder; The first mixed powder, a mixed solvent of acetone and chloroform mixed at a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMIM TFSI, 1-Ethyl- The first natural product comprising lotus seed extract and burdock seed extract, comprising the steps of extracting a solution containing the active ingredient by mixing 3-methylimidazolium bis (trifluoromethylsulfylimide); and concentrating and freeze-drying the extracted solution under reduced pressure. Preparing an extract;
    인동덩굴꽃, 지모뿌리 및 당개나리열매를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 인동덩굴꽃분말, 지모뿌리분말 및 당개나리열매분말을 포함하는 제2 혼합분말을 준비하는 단계; 상기 제2 혼합분말 및 물을 혼합하고, 교반을 수행하며 95-105℃의 온도에서 10-14시간 동안 1차 열수추출을 수행하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 135-145℃의 온도에서 4-8시간 동안 2차 열수추출을 수행하는 단계; 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조하는 단계; 및 상기 제1 추출물 및 제2 추출물을 혼합하는 단계;를 포함하는 인동덩굴꽃추출물, 지모뿌리추출물 및 당개나리열매추출물을 포함하는 제2 천연추출물을 제조하는 단계;Preparing a second mixed powder containing honeysuckle flower powder, root root powder, and forsythia fruit powder by washing honeysuckle flowers, root roots, and forsythia fruit, cutting them into appropriate sizes, freeze-drying, and pulverizing them; mixing the second mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105°C for 10-14 hours; After performing the first hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering through filter paper, mixing the remaining solids with water again, and performing the second hot water extraction at a temperature of 135-145°C for 4-8 hours; After secondary hot water extraction, filtering through filter paper and then freeze-drying the filtrate to prepare a second extract; and mixing the first extract and the second extract; preparing a second natural extract comprising a honeysuckle flower extract, a root extract, and a forsythia fruit extract;
    프로폴리스, 병풀 및 길경을 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 프로폴리스분말, 병풀분말 및 길경분말을 포함하는 제3 혼합분말을 준비하는 단계; 상기 제3 혼합분말 및 에탄올을 혼합하여 유효성분을 포함하는 용액을 추출하는 단계; 및 추출된 용액을 감압농축 및 동결건조하는 단계;를 포함하는 프로폴리스추출물, 병풀추출물 및 길경추출물을 포함하는 제3 천연추출물을 제조하는 단계;Preparing a third mixed powder containing propolis powder, centella asiatica powder, and apricot root by washing propolis, centella asiatica, and apricot root, cutting them into appropriate sizes, freeze-drying, and pulverizing them; extracting a solution containing an active ingredient by mixing the third mixed powder and ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure; preparing a third natural extract including propolis extract, Centella asiatica extract, and Gilge extract;
    상기 제1 천연추출물 28-32 중량부, 상기 제2 천연추출물 28-32 중량부 및 상기 제3 천연추출물 38-42 중량부를 혼합하여 천연복합추출물을 제조하는 단계; 및Preparing a natural complex extract by mixing 28-32 parts by weight of the first natural extract, 28-32 parts by weight of the second natural extract, and 38-42 parts by weight of the third natural extract; and
    글루타치온 0.3-0.7 중량부, 토코페롤 0.5-2 중량부, 상기 천연복합추출물 0.5-3 중량부, 아스코빅애씨드 9-20 중량부, 에칠아스코빌에텔 1-2 중량부 및 트라넥사믹애씨드 1-3 중량부를 포함하는 조성물을 제조하는 단계;를 포함하는 미백 및 항산화기능을 가지는 화장품 조성물의 제조방법.Glutathione 0.3-0.7 parts by weight, tocopherol 0.5-2 parts by weight, 0.5-3 parts by weight of the above natural complex extract, ascorbic acid 9-20 parts by weight, ethyl ascorbyl ether 1-2 parts by weight, and tranexamic acid 1-3 A method for producing a cosmetic composition having whitening and antioxidant functions, comprising: preparing a composition containing parts by weight.
PCT/KR2022/012106 2022-07-20 2022-08-12 Cosmetic composition having whitening and antioxidant functions WO2024019212A1 (en)

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