WO2023114932A3 - Subtilisin variants and uses thereof - Google Patents

Subtilisin variants and uses thereof Download PDF

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Publication number
WO2023114932A3
WO2023114932A3 PCT/US2022/081697 US2022081697W WO2023114932A3 WO 2023114932 A3 WO2023114932 A3 WO 2023114932A3 US 2022081697 W US2022081697 W US 2022081697W WO 2023114932 A3 WO2023114932 A3 WO 2023114932A3
Authority
WO
WIPO (PCT)
Prior art keywords
subtilisin
subtilisin variants
subtilisin variant
compositions
nucleic acid
Prior art date
Application number
PCT/US2022/081697
Other languages
French (fr)
Other versions
WO2023114932A2 (en
Inventor
Viktor Yuryevich Alekseyev
Lilia BABE
Lydia Dankmeyer
Frits Goedegebuur
Roopa GHIRNIKAR
Thijs Kaper
Harm Mulder
Nils Henning REDESTIG
Sander Van Stigt Thans
Original Assignee
Danisco Us Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc. filed Critical Danisco Us Inc.
Publication of WO2023114932A2 publication Critical patent/WO2023114932A2/en
Publication of WO2023114932A3 publication Critical patent/WO2023114932A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Abstract

Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
PCT/US2022/081697 2021-12-16 2022-12-15 Subtilisin variants and methods of use WO2023114932A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163290067P 2021-12-16 2021-12-16
US63/290,067 2021-12-16

Publications (2)

Publication Number Publication Date
WO2023114932A2 WO2023114932A2 (en) 2023-06-22
WO2023114932A3 true WO2023114932A3 (en) 2023-07-27

Family

ID=85172579

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2022/081697 WO2023114932A2 (en) 2021-12-16 2022-12-15 Subtilisin variants and methods of use

Country Status (1)

Country Link
WO (1) WO2023114932A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021081547A2 (en) * 2019-10-24 2021-04-29 The Procter & Gamble Company Automatic dishwashing detergent composition comprising a protease

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