WO2023098393A1 - 一种免疫检测的液路系统 - Google Patents

一种免疫检测的液路系统 Download PDF

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WO2023098393A1
WO2023098393A1 PCT/CN2022/129730 CN2022129730W WO2023098393A1 WO 2023098393 A1 WO2023098393 A1 WO 2023098393A1 CN 2022129730 W CN2022129730 W CN 2022129730W WO 2023098393 A1 WO2023098393 A1 WO 2023098393A1
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needle
liquid
pump
valve
reagent
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PCT/CN2022/129730
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English (en)
French (fr)
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王国锋
孟雄
凌赟
姚静岚
陈东鑫
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中翰盛泰生物技术股份有限公司
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Publication of WO2023098393A1 publication Critical patent/WO2023098393A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04—Cleaning involving contact with liquid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • the invention relates to the field of liquid circuit systems, in particular to a liquid circuit system for immunoassay.
  • the plunger pump is an important device in the hydraulic system. It relies on the reciprocating movement of the plunger in the cylinder to change the volume of the sealed working chamber to realize oil absorption and oil pressure.
  • the plunger pump has the advantages of high rated pressure, compact structure, high efficiency and convenient flow adjustment.
  • the hydraulic plunger pump relies on air pressure to supply oil to the hydraulic oil tank. After starting the machine every time, the hydraulic oil tank must reach the operating air pressure before operating the machine.
  • Straight-axis swash plate plunger pumps are divided into two types: pressure oil supply type and self-priming oil type. Most of the pressure oil supply hydraulic pumps use a fuel tank with air pressure, and some hydraulic pumps themselves have a charge pump to provide pressure oil to the oil inlet of the hydraulic pump.
  • the self-priming hydraulic pump has a strong self-priming ability and does not need external force to supply oil.
  • Patent CN 106546758 A discloses a fully automatic chemiluminescent immunoassay analyzer and its liquid circuit system, wherein the system uses a plunger pump and a solenoid valve to pick up and fill samples and pick up and fill luminescent acid and alkali solutions , and use the lotion to clean the inner wall of the sample needle; use the syringe and solenoid valve to pick up and fill the reagent; use two sets of syringes to achieve the ratio dilution of the concentrated lotion, and clean the outer wall of the sample needle and the inner and outer walls of the reagent needle;
  • the peristaltic pump handles the waste liquid from cleaning the magnetic bead cleaning module; the waste liquid diaphragm pump is used to handle the waste liquid generated by the system and the condensed water generated by the reagent cold storage.
  • This structure repeatedly sucks reagents and samples, which is likely to cause repeated pollution, and the conventional liquid system can only suck the inner wall, but cannot suck the
  • the object of the present invention is to provide a liquid circuit system for immunoassay in view of the defects in the prior art.
  • a liquid path system for immunoassay including a cleaning liquid path inside and outside a reagent needle, a clear water cleaning liquid path inside a sample needle, and a cleaning liquid path outside a sample needle;
  • the internal and external cleaning liquid path of the reagent needle includes a clean water bucket, a first pump, a first valve and a first needle washing pool connected in sequence, the reagent needle is arranged in the first needle washing pool, and the first valve is connected with the first needle washing pool all the way.
  • the plunger pump is connected to the inside of the reagent needle for cleaning the inner wall of the reagent needle, and the first valve is connected to the first needle washing pool for cleaning the outer wall of the reagent needle;
  • the clean water cleaning liquid path inside the sample needle includes a clean water bucket, a second pump, a second valve, a second plunger pump and a fourth valve connected in sequence, the second plunger pump is connected to the inside of the sample needle, and when the fourth valve is selected , the inner wall of the reagent needle is cleaned by the clean water cleaning liquid path inside the sample needle;
  • the washing liquid cleaning liquid circuit outside the sample needle includes a washing liquid barrel, a third pump, and a third valve connected in sequence.
  • the sample needle is arranged in the second needle washing pool, and the third valve is connected to the outer wall of the second needle washing pool. When the three valves are selected, the cleaning fluid path outside the sample needle cleans the outer wall of the sample needle.
  • the liquid circuit system also includes a condensate and waste liquid discharge circuit
  • the condensate and waste liquid discharge circuit includes a waste liquid needle, a fifth pump and a fifth valve connected in sequence, and the fifth valve One path is connected to the reagent plate, and the other path is connected to the waste liquid needle.
  • the fifth pump is used to discharge the condensate in the reagent plate and the waste liquid sucked by the waste liquid needle into the waste liquid bucket.
  • a fourth pump is connected to the bottom of the first needle washing pool, and the fourth pump is used to discharge the waste liquid from the first needle washing pool to the waste liquid bucket.
  • the bottom of the second needle washing pool is connected to a sixth pump, and the sixth pump is used to discharge the waste liquid from the second needle washing pool to the waste liquid barrel.
  • a filter and a connection joint are also provided between the clean water bucket and the first pump and the second pump.
  • a filter and a connection joint are also provided between the washing liquid bucket and the third pump.
  • a filter is provided between the reagent disc and the fifth valve.
  • the beneficial effects of the present invention are: the setting of multiple needles avoids the occurrence of cross-contamination during collection and addition, and the multi-channel cleaning liquid path is designed for the inner and outer walls of the sample needle and reagent needle to more thoroughly clean the double The needles are cleaned, so that the double needles will not be polluted twice during the repeated detection process, ensuring the safety of the detection.
  • Fig. 1 is a schematic diagram of a liquid circuit system for immunoassay provided by the present invention.
  • a liquid path system for immunoassay including a cleaning liquid path inside and outside the reagent needle 9, a clear water cleaning liquid path inside the sample needle 15, and a washing liquid cleaning liquid path outside the sample needle 15;
  • the internal and external cleaning liquid path of the reagent needle 9 includes a clear water bucket 20, a first pump 1, a first valve 2 and a first needle washing pool connected in sequence, the reagent needle 9 is arranged in the first needle washing pool, and the first needle washing pool is arranged in the first needle washing pool.
  • One way of the valve 2 is connected to the first plunger pump and connected to the inside of the reagent needle 9 for cleaning the inner wall of the reagent needle 9, and the other way of the first valve 2 is connected to the first needle washing pool for cleaning the outer wall of the reagent needle 9;
  • the clean water cleaning liquid path inside the sample needle 15 includes a clean water bucket 20, a second pump 4, a second valve 5, a second plunger pump 6, and a fourth valve 11 connected in sequence, and the second plunger pump 6 is connected to the sample needle 15.
  • the fourth valve 11 is gated, the inner wall of the sample needle 15 is cleaned with water to clean the liquid path and the inner wall of the reagent needle 9;
  • the washing liquid cleaning liquid circuit outside the sample needle 15 includes a washing liquid barrel 21, a third pump 7 and a third valve 8 connected in sequence.
  • the sample needle 15 is arranged in the second needle washing pool, and the third valve 8 is connected to the second The outer wall of the needle washing pool, when the third valve 8 is selected, the outer wall of the sample needle 15 is cleaned by the washing liquid cleaning liquid path outside the sample needle 15.
  • the liquid system also includes a condensate and waste liquid discharge circuit, the condensate and waste liquid discharge liquid circuit includes a waste liquid needle 17 connected in sequence, a fifth pump 12 and a fifth valve 13, the fifth valve One of 13 is connected to the reagent plate 16, and the other is connected to the waste liquid needle 17.
  • the fifth pump 12 is used to discharge the condensate in the reagent plate 16 and the waste liquid sucked by the waste liquid needle 17 into the waste liquid bucket 22.
  • the bottom of the first needle washing pool is connected to the fourth pump 10 , and the fourth pump 10 is used to discharge the waste liquid from the first needle washing pool to the waste liquid barrel 22 .
  • the bottom of the second needle washing pool is connected to the sixth pump 14 , and the sixth pump 14 is used to discharge the waste liquid from the second needle washing pool to the waste liquid barrel 22 .
  • a filter 19 and a connection joint 18 are also provided between the clean water bucket 20 and the first pump 1 and the second pump 4 .
  • a filter 19 and a connecting joint 18 are further provided between the washing liquid bucket 21 and the third pump 7 .
  • a filter 19 is provided between the reagent disc 16 and the fifth valve 13 .

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

一种免疫检测的液路系统，包括试剂针（9）内外清洗液路、样本针（15）内侧清水清洗液路、样本针（15）外侧洗液清洗液路；试剂针（9）内外清洗液路用于清洗试剂针（9）的内壁及清洗试剂针（9）的外壁；样本针（15）内侧清水清洗液路清洗样本针（15）的内壁；样本针（15）外侧洗液清洗液路清洗样本针（15）的外壁。多针头的设置避免采集、添加时交叉污染情况的发生，多通道清洗液路针对样本针（15）、试剂针（9）的内外两壁设计能更彻底的对双针进行清洗，使双针在反复的检测过程中不会发生二次污染，确保了检测的安全性。

Description

一种免疫检测的液路系统 技术领域
本发明涉及液路系统领域，尤其涉及一种免疫检测的液路系统。
背景技术
柱塞泵是液压系统的一个重要装置。它依靠柱塞在缸体中往复运动，使密封工作容腔的容积发生变化来实现吸油、压油。柱塞泵具有额定压力高、结构紧凑、效率高和流量调节方便等优点。
液压柱塞泵靠气压供油的液压油箱，在每次启动机器后，必须等液压油箱达到使用气压后，才能操作机械。直轴斜盘式柱塞泵分为压力供油型的自吸油型两种。压力供油型液压泵大都采用有气压的油箱，也有液压泵本身带有补油分泵向液压泵进油口提供压力油的。自吸油型液压泵的自吸油能力很强，无需外力供油。
专利CN 106546758 A公开了一种全自动化学发光免疫分析仪及其液路系统，其中，所述系统通过使用柱塞泵和电磁阀进行样本拾取和加注以及发光酸碱液的拾取和加注,并使用洗液进行样本针内壁清洗；使用注射器和电磁阀进行试剂拾取和加注；使用两组注射器实现浓缩洗液的配比稀释,并进行样本针外壁清洗、试剂针内外壁清洗；使用蠕动泵处理清洗磁珠清洗模块的废液；使用废液膜片泵处理系统产生的废液以及试剂冷藏仓产生的冷凝水。该结构反复抽吸试剂和样本，容易造成多次污染，且常规的液路系统只能吸内壁，无法吸外壁。
发明内容
本发明的目的是针对现有技术的缺陷，提供了一种免疫检测的液路系统。
为了实现以上目的，本发明采用以下技术方案：
一种免疫检测的液路系统，包括试剂针内外清洗液路、样本针内侧清水清洗液路、样 本针外侧洗液清洗液路；
所述试剂针内外清洗液路包括依次连通的清水桶、第一泵、第一阀及第一洗针池，所述试剂针设于第一洗针池内，所述第一阀一路与第一柱塞泵并连接试剂针内部用于清洗试剂针的内壁，第一阀另一路连接第一洗针池用于清洗试剂针的外壁；
所述样本针内侧清水清洗液路包括依次连通的清水桶、第二泵、第二阀、第二柱塞泵及第四阀，第二柱塞泵连接样本针内部，第四阀选通时，样本针内侧清水清洗液路清洗试剂针的内壁；
所述样本针外侧洗液清洗液路包括依次连通的洗液桶、第三泵及第三阀，所述样本针设于第二洗针池内，第三阀连接第二洗针池外壁，第三阀选通时，样本针外侧洗液清洗液路清洗样本针的外壁。
进一步的，所述液路系统还包括冷凝液、废液排放液路，所述冷凝液、废液排放液路包括依次连通的废液针，第五泵及第五阀，所述第五阀一路连接试剂盘，另一路连接废液针，所述第五泵用于将试剂盘内的冷凝液及废液针吸取的废液排放至废液桶中。
进一步的，所述第一洗针池底部连接第四泵，第四泵用于将第一洗针池的废液排放至废液桶。
进一步的，所述第二洗针池底部连接第六泵，第六泵用于将第二洗针池的废液排放至废液桶。
进一步的，所述清水桶与第一泵和第二泵之间还设有过滤器及连接接头。
进一步的，所述洗液桶与第三泵之间还设有过滤器及连接接头。
进一步的，所述试剂盘与第五阀之间设有过滤器。
采用本发明技术方案，本发明的有益效果为：多针头的设置避免采集、添加时交叉污染情况的发生，多通道清洗液路针对样本针、试剂针的内外两壁设计能更彻底的对双针进行清洗，使双针在反复的检测过程中不会发生二次污染，确保了检测的安全性。
附图说明
图1是本发明提供的一种免疫检测的液路系统的原理图。
其中，1、第一泵，2、第一阀，3、第一柱塞泵，4、第二泵，5、第二阀，6、第二柱塞泵，7、第三泵，8、第三阀，9、试剂针，10、第四泵，11、第四阀，12、第五泵，13、第五阀，14、第六泵，15、样本针，16、试剂盘，17、废液针，18、接头，19、过滤器，20、清水桶，21、洗液桶，22、废液桶。
具体实施方式
结合附图对本发明具体方案具体实施例作进一步的阐述。
如图所示，一种免疫检测的液路系统，包括试剂针9内外清洗液路、样本针15内侧清水清洗液路、样本针15外侧洗液清洗液路；
所述试剂针9内外清洗液路包括依次连通的清水桶20、第一泵1、第一阀2及第一洗针池，所述试剂针9设于第一洗针池内，所述第一阀2一路与第一柱塞泵并连接试剂针9内部用于清洗试剂针9的内壁，第一阀2另一路连接第一洗针池用于清洗试剂针9的外壁；
所述样本针15内侧清水清洗液路包括依次连通的清水桶20、第二泵4、第二阀5、第二柱塞泵6及第四阀11，第二柱塞泵6连接样本针15内部，第四阀11选通时，样本针15内侧清水清洗液路清洗试剂针9的内壁；
所述样本针15外侧洗液清洗液路包括依次连通的洗液桶21、第三泵7及第三阀8，所述样本针15设于第二洗针池内，第三阀8连接第二洗针池外壁，第三阀8选通时，样本针15外侧洗液清洗液路清洗样本针15的外壁。
所述液路系统还包括冷凝液、废液排放液路，所述冷凝液、废液排放液路包括依次连通的废液针17，第五泵12及第五阀13，所述第五阀13一路连接试剂盘16，另一路连接废液针17，所述第五泵12用于将试剂盘16内的冷凝液及废液针17吸取的废液排放至废液桶22中。
所述第一洗针池底部连接第四泵10，第四泵10用于将第一洗针池的废液排放至废液桶22。
所述第二洗针池底部连接第六泵14，第六泵14用于将第二洗针池的废液排放至废液桶22。
所述清水桶20与第一泵1和第二泵4之间还设有过滤器19及连接接头18。
所述洗液桶21与第三泵7之间还设有过滤器19及连接接头18。
所述试剂盘16与第五阀13之间设有过滤器19。
注意，上述仅为本发明的较佳实施例及所运用技术原理。本领域技术人员会理解，本发明不限于这里所述的特定实施例，对本领域技术人员来说能够进行各种明显的变化、重新调整和替代而不会脱离本发明的保护范围。因此，虽然通过以上实施例对本发明进行了较为详细的说明，但是本发明不仅仅限于以上实施例，在不脱离本发明构思的情况下，还可以包括更多其他等效实施例，而本发明的范围由所附的权利要求范围决定。

Claims (7)

  1. 一种免疫检测的液路系统，其特征在于，包括试剂针内外清洗液路、样本针内侧清水清洗液路、样本针外侧洗液清洗液路；
    所述试剂针内外清洗液路包括依次连通的清水桶、第一泵、第一阀及第一洗针池，所述试剂针设于第一洗针池内，所述第一阀一路与第一柱塞泵并连接试剂针内部用于清洗试剂针的内壁，第一阀另一路连接第一洗针池用于清洗试剂针的外壁；
    所述样本针内侧清水清洗液路包括依次连通的清水桶、第二泵、第二阀、第二柱塞泵及第四阀，第二柱塞泵连接样本针内部，第四阀选通时，样本针内侧清水清洗液路清洗试剂针的内壁；
    所述样本针外侧洗液清洗液路包括依次连通的洗液桶、第三泵及第三阀，所述样本针设于第二洗针池内，第三阀连接第二洗针池外壁，第三阀选通时，样本针外侧洗液清洗液路清洗样本针的外壁。
  2. 如权利要求1所述的一种免疫检测的液路系统，其特征在于，所述液路系统还包括冷凝液、废液排放液路，所述冷凝液、废液排放液路包括依次连通的废液针，第五泵及第五阀，所述第五阀一路连接试剂盘，另一路连接废液针，所述第五泵用于将试剂盘内的冷凝液及废液针吸取的废液排放至废液桶中。
  3. 如权利要求2所述的一种免疫检测的液路系统，其特征在于，所述第一洗针池底部连接第四泵，第四泵用于将第一洗针池的废液排放至废液桶。
  4. 如权利要求2所述的一种免疫检测的液路系统，其特征在于，所述第二洗针池底部连接第六泵，第六泵用于将第二洗针池的废液排放至废液桶。
  5. 如权利要求1所述的一种免疫检测的液路系统，其特征在于，所述清水桶与第一泵和第二泵之间还设有过滤器及连接接头。
  6. 如权利要求1所述的一种免疫检测的液路系统，其特征在于，所述洗液桶与第三泵之间还设有过滤器及连接接头。
  7. 如权利要求2所述的一种免疫检测的液路系统，其特征在于，所述试剂盘与第五阀之间设有过滤器。
PCT/CN2022/129730 2021-12-03 2022-11-04 一种免疫检测的液路系统 WO2023098393A1 (zh)

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CN202123031550.4 2021-12-03
CN202123031550.4U CN217385525U (zh) 2021-12-03 2021-12-03 一种免疫检测的液路系统

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