WO2023097963A1 - Automatic preparation device for red cell suspension and preparation method - Google Patents

Abstract

An automatic preparation device (100) for a red cell suspension and a preparation method. The automatic preparation device (100) for a red cell suspension comprises a centrifugal device (1), a suction device (2), a replenishment device (4), an identification device (3), and a control device for controlling them. The centrifugal device (1) comprises centrifugal chambers (11a) and a driving device. The identification device (3) comprises a first identification device (31) disposed in the suction device (2) and second identification devices (32) disposed in the centrifugal chambers (11a). The driving device drives the centrifugal chambers (11a) to perform a centrifugal motion so that the liquid inside the centrifugal chambers (11a) is centrifuged and layered; the first identification device (31) identifies and triggers a first signal to the control device, and the control device controls the suction device to suction the layered liquid; the second identification devices (32) trigger a second signal to the control device, then the control device controls the suction device (2) to stop working and controls the replenishment device (4) to add a saline solution to the centrifugal chambers (11a).

Description

A kind of red blood cell suspension automatic production device and production method

This application claims the priority of the Chinese patent application with application number 202111461594.2 filed on December 02, 2021, the entire contents of which are hereby incorporated by reference.

technical field

The present application relates to the technical field of production of red blood cell suspension, in particular to an automatic production device and method of red blood cell suspension.

Background technique

In the production of the existing erythrocyte suspension, the first step is to centrifuge the whole blood against coagulation, add physiological saline after centrifugation, mix well, remove the upper layer of living fluid and then centrifuge, repeat this step until the upper living fluid after centrifugation is transparent, and the pressure is obtained. accumulation of red blood cells. Then draw one drop of packed red blood cells and nine drops of normal saline and mix evenly to obtain a 10% red blood cell suspension.

However, the preparation of the existing red blood cell suspension requires several key steps such as adding blood samples, adding normal saline, centrifuging blood samples, and extracting packed red blood cells. Operational accuracy and increased fatigue are not conducive to mass production. In addition, repeated operations are likely to reduce configuration efficiency and accuracy, and produce colonies to contaminate the red blood cell suspension.

The process from the centrifugation of whole blood to the formation of packed red blood cells will be accompanied by multiple contacts between the blood sample and bacteria in the air, resulting in the attachment and growth of bacterial colonies in the blood sample, contaminating the red blood cell suspension; currently, it is usually manually operated by the operator Different concentration of blood samples and normal saline are drawn in different ways, and centrifuges with different speeds are arranged according to different blood samples. Once the demand for suspension liquid configuration increases, it will greatly increase the workload of the operator. In the case of multiple operations The lower accuracy is difficult to guarantee; the process of preparing the red blood cell suspension needs to centrifuge the whole blood multiple times, and remove the supernatant after centrifugation. Living fluids (discarded plasma, red blood cells, and cell fluids) pollute the environment and must be inactivated before they can be discharged into the natural environment; currently, auxiliary instruments such as pipettes and test tubes used in the preparation process of red blood cell suspensions are After the suspension is prepared, it must be cleaned and sterilized before it can be used again. At present, most of them are realized by manual cleaning combined with special disinfection equipment sterilization. Since this process requires manual transfer of instruments, the efficiency is low, and the transfer process is easy to be stained with bacteria.

technical problem

The main purpose of this application is to propose an automatic red blood cell suspension production device and production method, which aims to realize the automation of multiple key steps in the production of red blood cell suspension, save the waiting time for manual operations and greatly improve the production efficiency. Suspension batch production provides technical support.

technical solution

In order to achieve the above object, the application proposes a red blood cell suspension automatic production device, including: a centrifugal device, including a reaction box, a driving device and a reaction bottle, a reaction chamber is formed in the reaction box, and the reaction bottle is The axis in the height direction of the box is rotatably set in the reaction chamber, a centrifugal chamber is formed in the reaction bottle, and the driving device drives the reaction bottle to rotate to drive the liquid in the centrifugal chamber to perform centrifugal movement for layered;

A suction device, including a suction part movably installed on the reaction box in the direction of approaching and away from the centrifugal cavity, and a driving part for driving the suction part, so as to drive the The suction part is close to the centrifugal cavity to suck the liquid in the centrifugal cavity;

A supply device, configured to add physiological saline into the centrifuge chamber;

The identification device includes a first identification device provided on the suction part and a second identification device provided at the bottom of the centrifugal chamber, the first identification device senses that the liquid in the centrifugal chamber has completed centrifugation The first signal is triggered after the first layer, and the second identification device triggers the second signal after sensing that the suction part performs suction; and,

a control device, electrically connected to the centrifugal device, the suction device, the replenishment device and the identification device, so as to drive the suction device close to the centrifugal cavity when receiving the first signal , and when the second signal is received, the suction device is stopped and the replenishment device is driven to add physiological saline to the centrifuge cavity.

In one embodiment, the suction device includes a movable sleeved fixed cylinder and a movable cylinder, and the movable cylinder has a movable stroke close to or away from the centrifugal chamber;

The suction device also includes a first piston rod, an air pump, and an accumulator, and the first piston rod is movably arranged in the fixed cylinder to separate the fixed cylinder into a section close to the centrifugal chamber. The first cavity segment and the second cavity segment away from the centrifugal cavity; the air pump supplies air to the second cavity segment to drive the first piston rod to move close to the centrifugal cavity, and the accumulator supplies energy to the centrifugal cavity. supply air to the first chamber section to drive the first piston rod to move away from the centrifugal chamber;

Wherein, the first piston rod is connected to an end of the movable cylinder away from the centrifugal cavity.

In one embodiment, two limit switches are arranged at intervals along the direction close to the centrifugal chamber on the fixed cylinder, and the first piston rod reciprocates between the two limit switches, and moves along the A signal is triggered when moving in one direction until the limit switch is touched, and the control device controls the reverse movement of the piston rod when receiving the trigger signal.

In one embodiment, the suction device further includes:

The second piston rod is movably arranged in the movable cylinder, so as to separate the movable cylinder into a third cavity segment close to the centrifugal cavity and a fourth cavity segment away from the centrifugal cavity;

The first drive mechanism is configured to drive the second piston rod to move, the drive mechanism includes a gear and a movable rack, the second piston rod is connected to the rack, and the gear rotates to drive the rack a bar gradually moves toward or away from the centrifuge chamber to move the second piston rod toward or away from the centrifuge chamber; and,

A needle tube communicated with the fourth chamber segment, and one end extends toward the direction of the centrifuge chamber to extend out of the fourth chamber segment;

Wherein, the first identification device is arranged at one end of the fourth chamber segment close to the centrifugal chamber.

In one embodiment, a thermal resistance wire is provided on the inner wall of the fourth chamber section, configured to heat the liquid in the fourth chamber section.

In one embodiment, the first cavity is provided with an air intake channel, and the air intake channel is provided with a first control valve; and/or,

A first passage is provided between the first cavity and the air pump, and a second control valve is provided on the first passage; and/or,

A second channel is provided between the second cavity and the accumulator, and a third control valve is provided on the second channel; and/or,

A liquid outlet channel is provided on the third cavity, and a fourth control valve is provided on the liquid outlet channel; and/or,

The needle tube is provided with a fifth control valve.

In one embodiment, the reaction chamber is provided with a plurality of reaction bottles, each of the reaction bottles includes a bottle body and a bottle cap, and the bottle cap is configured to seal the bottle body, and the centrifuge device also includes A linkage mechanism and a drive motor, each of the bottle caps is connected to the drive motor through a linkage mechanism, and the drive motor drives the movement of the linkage mechanism so that each of the bottle caps moves to open and close the bottle.

In one embodiment, the replenishment device includes:

A liquid storage tank is movably arranged on the top of the centrifugal chamber along a direction close to or away from the centrifugal chamber, and is configured to hold physiological saline;

a pipe configured to communicate with the liquid storage tank and the centrifuge chamber; and,

The second driving mechanism is arranged outside the liquid storage tank and is configured to drive the liquid storage tank to move close to or away from the centrifugal chamber, and the liquid storage tank moves to realize the connection between the liquid storage tank and the centrifugal chamber. The liquid level difference of the liquid changes, so that the liquid in the liquid storage tank is input into the centrifugal chamber through the pipeline.

In one embodiment, the red blood cell suspension automatic preparation device further includes a heating device, which is arranged on the outside of the reaction bottle and configured to heat the liquid in the centrifuge chamber.

Correspondingly, the present application also proposes a manufacturing method based on an automatic red blood cell suspension production device, and the production method of the red blood cell suspension includes the following steps:

controlling the centrifugal device to perform centrifugal stratification on the centrifuge cavity filled with anticoagulated whole blood;

receiving the first signal sent by the first identification device, controlling the suction device to suck the upper liquid level in the centrifuge chamber;

receiving the second signal sent by the second identification device, controlling the suction device to stop suction, and controlling the replenishment device to inject physiological saline into the centrifuge cavity to obtain a suspension of red blood cells;

After the red blood cell suspension in the centrifuge cavity is taken out, the replenishment device is controlled to fill the centrifuge cavity with physiological saline;

The suction device is controlled to suck the physiological saline in the centrifugal cavity, so as to discharge the physiological saline in the suction device.

Beneficial effect

In the technical solution of the present application, the control device controls the driving device to drive the reaction bottle to rotate to stratify the liquid in the centrifuge chamber, and the control device receives the triggering of the first identification device After receiving the first signal, the suction part is controlled to suck the liquid in the centrifuge cavity, and the control device controls the pumping part after receiving the second signal triggered by the second identification device. The suction device is stopped and the replenishment device is controlled to add physiological saline into the centrifuge chamber to obtain a suspension of red blood cells. The identification device transmits signals to the control device for automatic control, completes the automatic production of red blood cell suspension, realizes the automation function of multiple key steps in the production of red blood cell suspension, saves the waiting time for manual operation and greatly improves the production efficiency, and provides red blood cell suspension. Liquid mass production to provide technical support.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present application, and those skilled in the art can also obtain other drawings according to the structures shown in these drawings without creative effort.

Fig. 1 is the overall schematic diagram of an embodiment of the red blood cell suspension automatic production device provided by the present application;

Fig. 2 is the structural representation of centrifugal device in Fig. 1;

Fig. 3 is a schematic structural view of the suction device in Fig. 1;

Fig. 4 is a schematic structural diagram of the replenishing device in Fig. 1 .

Explanation of reference numbers:

label	name	label	name
100	Red blood cell suspension automatic production device	223	Needle
1	centrifugal device	224	third chamber segment
11	Reaction bottle	225	fourth chamber segment
11a	centrifuge chamber	226	Thermal resistance wire
12	Linkage	twenty three	first control valve
13	motor	twenty four	Second control valve
2	suction device	25	third control valve
twenty one	Fixed cylinder	26	Fourth control valve
211	first piston rod	27	fifth control valve
212	air pump	3	identification device
213	Accumulator	31	first identification device
214	first chamber segment	32	Second identification device
215	second chamber segment	4	supply device
216	limit switch	41	Liquid storage tank
twenty two	Active cylinder	42	pipeline
221	second piston rod	43	second drive mechanism
222	first drive mechanism	5	heating equipment

The realization, functional features and advantages of the present application will be further described in conjunction with the embodiments and with reference to the accompanying drawings.

Embodiments of the present invention

The following will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of them. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of this application.

It should be noted that if there are directional indications (such as up, down, left, right, front, back...) in the embodiment of the present application, the directional indications are only used to explain the position in a certain posture (as shown in the attached figure). If the specific posture changes, the directional indication will also change accordingly.

In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present application, the descriptions of "first", "second", etc. are only for descriptive purposes, and cannot be interpreted as indications or hints Its relative importance or implicitly indicates the number of technical features indicated. Thus, the features defined as "first" and "second" may explicitly or implicitly include at least one of these features. In addition, the meaning of "and/or" appearing in the whole text includes three parallel schemes, taking "A and/or B" as an example, including scheme A, scheme B, or schemes that both A and B satisfy. In addition, the technical solutions of the various embodiments can be combined with each other, but it must be based on the realization of those skilled in the art. When the combination of technical solutions is contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist , nor within the scope of protection required by the present application.

The preparation process of the existing erythrocyte suspension involves multiple key steps such as adding blood samples, adding normal saline, centrifuging blood samples, and extracting packed red blood cells. Currently, these operating steps are mostly carried out manually, and repeated operations are easy to reduce the operating cost. Accuracy and increased fatigue are not conducive to mass production. An automated and intelligent device is urgently needed to realize the integrated production of red blood cell suspension. In view of this, this application designs an automatic production device for red blood cell suspension, which saves manual operation and waiting The time is greatly improved and the production efficiency is greatly improved, providing technical support for the mass production of red blood cell suspension.

Please refer to FIG. 1 to FIG. 4 , which are an embodiment of an automatic red blood cell suspension production device proposed in this application.

Please refer to Fig. 1 to Fig. 2, a kind of red blood cell suspension automatic preparation device 100 comprises centrifugal device 1, suction device 2, replenishment device 4, identification device 3 and control device, and described centrifugal device 1 comprises reaction box, driving device and Reaction bottle 11, a reaction cavity is formed in the reaction box, and the reaction bottle 11 is rotatably arranged in the reaction cavity along the axis of the height direction of the reaction box, and a centrifugal cavity 11a is formed in the reaction bottle 11, so The driving device drives the reaction bottle 11 to rotate, so as to drive the liquid in the centrifugal chamber 11a to perform centrifugal movement for stratification; the suction device 2 includes a movable The suction part installed in the reaction box, and the driving part that drives the suction part to move, so that the suction part can be driven to approach the centrifugal chamber 11a on the driving part to the liquid in the centrifugal chamber 11a Suction; the replenishment device 4 is set to add physiological saline to the centrifuge cavity 11a; the identification device 3 includes a first identification device 31 located at the suction part and a first identification device 31 located at the centrifuge cavity 11a The second identification device 32 at the bottom, the first identification device 31 triggers the first signal after sensing that the liquid in the centrifuge chamber 11a completes centrifugal stratification, and the second identification device 32 senses that the The suction part triggers the second signal after sucking; the control device is electrically connected with the centrifugal device 1, the suction device 2, the replenishment device 4 and the identification device 3, so that when receiving When the first signal is used, the suction device 2 is driven close to the centrifugal cavity 11a, and when the second signal is received, the suction device 2 is stopped and the replenishment device 4 is driven to add physiological saline to the centrifugal cavity 11a.

In this embodiment, the control device controls the drive device to drive the reaction bottle 11 to rotate to stratify the liquid in the centrifuge chamber 11a, and the control device receives the first identification device 31 After the first signal is triggered, the suction part is controlled to suck the liquid in the centrifugal chamber 11a, and the control device receives the second signal triggered by the second identification device 32 , control the suction device 2 to stop the activity and control the replenishment device 4 to add physiological saline into the centrifuge chamber 11a to obtain red blood cell suspension. The identification device 3 transmits signals to the control device for automatic control, completes the automatic production of the red blood cell suspension, realizes the automation of multiple key steps in the production of the red blood cell suspension, saves the waiting time for manual operations and greatly improves the production efficiency. Provide technical support for mass production of suspensions.

It is worth noting that both the first identification device 31 and the second identification device 32 include a photoelectric liquid level switch, and the photoelectric liquid level switch includes a light emitting element and a receiving element, and the light emitting element and the receiving element can be It is a light-emitting crystal and a receiving crystal, or other elements capable of emitting light and receiving, which are not limited here. Using the principle of reflection and refraction of light at the interface of two different media, the receiving element senses the intensity difference of the light reflected from the light emitting element, and judges whether the preset condition is met and takes action. The specific principle is that the first identification device 31 continuously detects the upper surface of the whole blood in the centrifuge cavity 11a, and the light-emitting element emits light. At this time, the intensity of the reflected light signal received by the receiving element is Q1. When the suction part of the suction device 2 contacts the upper surface of the whole blood, the intensity of the reflected light signal received by the receiving element is Q2, and when the intensity of the received signal changes from Q1 to Q2, it is equivalent to triggering the first Once a signal is received, the control device starts to control the suction part to perform suction; the second identification device 32 continues to detect the layered liquid in the centrifugal chamber 11a, and the light-emitting element emits light. When there are two layers of layered liquid, the intensity of the reflected light signal received by the receiving element is Q3; when only one layer of liquid is detected, the intensity of the reflected light signal received by the receiving element is Q4; when the intensity of the received signal is When changing from Q3 to Q4, it is equivalent to triggering the second signal, and the control device controls the suction part to stop suction and controls the supply device to supply physiological saline.

The first recognition device 31 recognizes by the difference of light refraction between the air and the liquid surface and then transmits a signal to the control device, and the second recognition device 32 uses the difference of light refraction of light between different liquid surfaces Identify and then transmit a signal to the control device, and then control the action of the suction part to perform suction or stop suction. The centrifuge chamber 11a is in a sealed state during centrifugation to prevent the centrifuged liquid from flying out of the centrifuge chamber 11a during centrifugation. During the suction and replenishment process, the inside of the reaction chamber is also in a sealed state to prevent the blood sample from entering the air. Multiple exposures of bacteria, resulting in the attachment and growth of colonies in the blood sample, contaminate the erythrocyte suspension.

Referring to Fig. 3, the suction device 2 includes a movable socketed fixed cylinder 21 and a movable cylinder 22, and the movable cylinder 22 has a movable stroke close to or away from the centrifugal chamber 11a; 2 also includes a first piston rod 211, an air pump 212 and an accumulator 213, the first piston rod 211 is movably arranged in the fixed cylinder 21, so as to separate the inside of the fixed cylinder 21 from the The first chamber section 214 of the centrifugal chamber 11a and the second chamber section 215 away from the centrifugal chamber 11a; the air pump 212 supplies air to the second chamber section 215 to drive the first piston rod 211 close to the The centrifugal cavity 11a moves, and the accumulator 213 supplies air to the first cavity segment 214 to drive the first piston rod 211 to move away from the centrifugal cavity 11a; wherein, the first piston rod 211 and the The movable cylinder 22 is connected to one end away from the centrifugal chamber 11a.

In this embodiment, the air pump 212 moves the first piston rod 211 close to the centrifugal cavity 11a, and the gas of the air pump 212 will enter the accumulator after driving the first piston rod 211 to move. 213 for storage, when the first piston rod 211 moves to the preset position, the accumulator 213 drives the first piston rod 211 to move away from the centrifugal chamber 11a, the air pump 212 and the accumulator 213 The design is not only environmentally friendly but also saves resources. The fixed cylindrical body 21 and the movable cylindrical body 22 are designed to be movably socketed in sequence, the fixed cylindrical body 21 and the movable cylindrical body 22 are connected through the first piston rod 211, and the first piston rod 211 The movable cylinder 22 is pushed close to the liquid surface for suction, and the movable cylinder 22 forms the suction part. The separated two-section cylinder is not only convenient for control, but also easy to disassemble, clean and replace.

Further, two limit switches 216 are arranged at intervals on the fixed cylinder 21 along the direction close to the centrifugal cavity 11a, and the first piston rod 211 reciprocates between the two limit switches 216, And move in one direction until the limit switch 216 is touched to trigger a signal, and the control device controls the piston rod to move backward when receiving the trigger signal.

In this embodiment, limit switches 216 are provided on the fixed cylinder 21, and the positions of the two limit switches 216 can be adjusted according to actual conditions, so that the movable stroke of the first piston rod 211 can be adjusted. control, when the suction part is sucking, the first piston rod 211 moves close to the centrifugal chamber 11a for suction, and the control device controls the suction according to the signal transmitted by the first identification device 31 The first piston rod 211 gradually moves closer to the centrifugal cavity 11a until the piston rod 211 reaches a limit switch close to the centrifugal cavity 11a, and the control device controls the first piston rod 211 Moving away from the centrifugal chamber 11a, when reaching the limit switch away from the centrifugal chamber 11a, the control device will judge whether to continue to control the movement of the first piston rod 211 in the opposite direction. The control device controls the reverse movement of the piston rod when receiving the signal triggered by the travel switch to perform intelligent control, and prevents the blood sample from contacting the air to contaminate the red blood cell suspension during control.

Please refer to FIG. 3, the suction device 2 also includes a second piston rod 221, a first driving mechanism 222 and a needle tube 223, and the second piston rod 221 is movably arranged in the movable cylinder 22, so as to The movable cylinder 22 separates a third chamber section 224 close to the centrifugal chamber 11a and a fourth chamber section 225 away from the centrifugal chamber 11a; the first driving mechanism 222 is configured to drive the second piston rod 221 movement, the drive mechanism includes a gear and a movable rack, the second piston rod 221 is connected to the rack, and the gear rotates to drive the rack to gradually approach or move away from the centrifugal cavity 11a to move Move the second piston rod 221 close to or away from the centrifugal cavity 11a; the needle tube 223 communicates with the fourth cavity section 225, and one end extends toward the direction of the centrifugal cavity 11a to extend out of the fourth cavity. outside the chamber segment 225; wherein, the first identifying device 31 is disposed in the fourth chamber segment 225 near one end of the centrifugal chamber 11a.

In this embodiment, the movable cylinder 22 is used as a needle to suck the upper liquid surface after stratification, and the second piston rod 221 is movably arranged in the movable cylinder 22, and the The movable cylinder 22 is divided into the third chamber section 224 and the fourth chamber section 225. During suction, the first driving mechanism 222 drives the first piston rod 211 to move close to the centrifugal chamber 11a first. , at this moment, the volume of the third chamber section 224 approximates the volume of the entire movable cylinder 22, and then the first driving mechanism 222 drives the first piston rod 211 to move away from the centrifugal chamber 11a, so that the Negative pressure is formed at the fourth chamber section 225 and the needle tube 223 to suck the liquid in the centrifugal chamber 11a. The first driving mechanism 222 includes a gear and a rack, and the gear and the rack are controlled to mesh to control the feeding amount, so as to facilitate more accurate suction movement and prevent excessive suction. The first driving mechanism may also include two gears meshing with each other, and it only needs to be able to precisely control the movement amount of the second piston rod 221 , which is not limited here. The first identification device 31 is arranged at one end of the fourth cavity section 225 close to the centrifugal cavity 11a, so as to facilitate the identification by the first identification device 31 .

Further, the inner wall of the fourth chamber section 225 is provided with a thermal resistance wire 226 configured to heat the liquid in the fourth chamber section 225 .

In this embodiment, a thermal resistance wire 226 is provided on the inner wall of the fourth chamber section 225, and the thermal resistance wire 226 after being energized can perform high-temperature inactivation treatment on the waste liquid in the fourth chamber section 225, Avoid polluting the environment when the waste liquid is discharged. At the same time, the thermal resistance wire 226 can also perform high-temperature treatment on the inside of the fourth chamber section 225 when not being sucked, so as to purify the environment in the fourth chamber section 225 . In this application, the device for high-temperature inactivation may also be other devices with heat inactivation effect, such as an infrared heating device, and is not limited to using the thermal resistance wire 226 for heat inactivation.

Please refer to FIG. 3 , the first cavity is provided with an air intake passage, and the first control valve 23 is provided on the air intake passage; and/or, a A first channel, the first channel is provided with a second control valve 24; and/or, a second channel is provided between the second cavity and the accumulator 213, and a second channel is provided on the second channel There is a third control valve 25; and/or, the third cavity is provided with a liquid outlet channel, and the fourth control valve 26 is provided on the liquid outlet channel; and/or, the needle tube 223 is provided with a first Five control valves 27 .

In this embodiment, the first control valve 23 is set to seal the first cavity section 214, so that the air pump 212 and the accumulator 213 can drive the first piston rod 211 to move, and the second The air pump 212 supplies air to the first chamber section 214 after the control valve 24 is opened, and the accumulator 213 supplies air to the second chamber section 215 after the third control valve 25 is opened. In order to facilitate the discharge of the liquid in the second cylinder, a liquid outlet channel communicating with the outside world is designed on the second cylinder body, and the fourth control valve 26 controls the opening and closing of the liquid outlet channel. The fifth control valve 27 is arranged on the needle tube 223 and prohibits or discharges the liquid in the needle tube 223 . Designing multiple control valves can make the suction device 2 more convenient to control during suction, can adapt to the needs of more suction situations, and make the red blood cell suspension automatic production device 100 more intelligent. The multiple control valves It is electrically communicated with the control device for easy control. The specific control method is that the first identification device 31 starts to identify, and after the first signal is triggered, the second control valve 24 is opened, the third control valve 25 is closed, and the first piston rod 211 approaches The centrifugal chamber 11a moves; after the second signal is triggered, the first control valve 23 is closed, the second control valve 24 is closed, the third control valve 25 is opened, and the first piston rod 211 is away from the The centrifugal chamber 11a moves. It is worth noting that the specific form of the control valve is not limited, and it may be a solenoid valve or other electric valves.

Referring to Fig. 1 and Fig. 2, a plurality of reaction vials 11 are arranged in the reaction chamber, and each of the reaction vials 11 includes a bottle body and a bottle cap, and the bottle cap is configured to seal the bottle body, the The centrifugal device 1 also includes a link mechanism 12 and a drive motor 13, each of the bottle caps is connected to the drive motor 13 through the link mechanism 12, and the drive motor 13 drives the link mechanism 12 to move, so that Each of the bottle caps is movable to open and close the bottle body.

In this embodiment, the reaction chamber is cylindrical, and two reaction vials 11 are arranged opposite to each other along the radial direction of the reaction chamber in the reaction chamber, and the corresponding two reaction vials 11 can make the centrifuge The device 1 makes the objects in the centrifugal device 1 more stable when performing centrifugal motion. In the present application, the number of the reaction bottles 11 in the reaction chamber is an even number and they are arranged symmetrically in the reaction chamber, so as to improve the stability of the objects in the centrifuge chamber 11a. At the same time, in this embodiment, the bottle cap is used as a method of sealing the bottle body, and the driving motor 13 and the linkage mechanism 12 are used as a mechanism for driving the bottle cap to seal the bottle body. The two seal the bottle body together. In other embodiments, a detachable sealing bag can also be used for sealing. The method of sealing the reaction bottle 11 is not specifically limited here.

Please refer to FIG. 4 , the replenishment device 4 includes a liquid storage tank 41 , a pipeline 42 and a second drive mechanism 43 , and the liquid storage tank 41 is movably arranged in the centrifugal chamber 11a in a direction close to or away from the centrifugal chamber 11a. The top of the cavity 11a is set to hold physiological saline; the pipeline 42 is set to communicate with the liquid storage tank 41 and the centrifugal cavity 11a; the second driving mechanism 43 is arranged outside the liquid storage tank 41 and is set to Drive the liquid storage tank 41 to move close to or away from the centrifugal cavity 11a, and the liquid storage tank 41 moves to realize the change of the liquid level difference between the liquid storage tank 41 and the liquid in the centrifugal cavity 11a, so that the The liquid in the liquid storage tank 41 is input to the centrifugal chamber 11a through the pipeline 42 .

In this embodiment, the liquid in the liquid storage tank 41 is input into the reaction chamber by using the siphon principle, the pipeline 42 is a U-shaped siphon tube, and the second driving mechanism 43 drives the liquid storage tank 41 moves to transport the liquid in the liquid storage tank 41 to the centrifugal cavity 11a by using the liquid level difference. Since the amount of physiological saline required to prepare the erythrocyte suspension is mostly measured in milliliters, precise micro-feeding is required, so the second driving mechanism 43 is a rack and pinion mechanism, and the rack and the liquid storage tank 41 connected, the gear drives the rack to move closer to or away from the centrifugal cavity 11a to drive the liquid storage tank 41 to move, and the feed per tooth of the gear and the cross-sectional area of the liquid storage tank 41 need to be increased Calculate the number of teeth that the driving gear needs to rotate, and ensure the accuracy of the amount of added physiological saline through the accurately calculated feed amount. In the present application, the specific form of the second driving mechanism 43 is not limited, and it can also be two gears for meshing transmission, or a threaded screw drive, as long as it can ensure the accurate feeding of physiological saline.

Please refer to Fig. 1 and Fig. 2, described erythrocyte suspension automatic preparation device 100 also comprises heating device 5, and described heating device 5 is arranged on the outside of described reaction bottle 11, is set to the liquid in described centrifugal chamber 11a heating.

In this embodiment, the heating device 5 is a thermal resistance wire 226, and the heating device 5 is set to inactivate or sterilize specific substances in the centrifugal cavity 11a at high temperature. In this application, the heating device 5 The specific form of the device 5 is not limited, and it may also be a pipeline heater, an infrared heater, or the like.

The present application also proposes a method for making a red blood cell suspension based on the automatic red blood cell suspension production device 100, which specifically includes the following steps:

Controlling the centrifuge device 1 to centrifugally stratify the centrifuge cavity 11a filled with anticoagulated whole blood;

receiving the first signal sent by the first identification device 31, controlling the suction device 2 to suck the upper liquid level in the centrifugal cavity 11a;

After receiving the second signal sent by the second identification device 32, control the suction device 2 to stop suction, and control the replenishment device 4 to inject physiological saline into the centrifuge cavity 11a, so as to obtain red blood cells suspension;

After the red blood cell suspension in the centrifuge chamber 11a is taken out, control the replenishment device 4 to fill the centrifuge chamber 11a with physiological saline;

The suction device 2 is controlled to suck the physiological saline in the centrifugal cavity 11 a, so as to discharge the physiological saline in the suction device 2 .

Specifically, in this embodiment, the centrifuge device 1 includes a cylindrical cavity, and two reaction vials 11 are respectively arranged in the cavity along the two sides of the central axis of the cavity (the reaction vials 11 are provided with centrifuge chamber 11a, hereafter referred to as the first centrifuge chamber and the second centrifuge chamber), the reaction bottle 11 includes a bottle body and a bottle cap, the bottle cap is set to seal the bottle body, and the linkage mechanism 12 is respectively connected The bottle caps of the two reaction bottles 11, the driving motor 13 drives the bottle caps to seal or contact to seal the bottle body. After the centrifugal stratification, the suction device 2 approaches the first centrifugal chamber, the first recognition device 31 starts to identify, confirms that the first signal is received, and the suction part starts to suck. The second identifying device 32 starts to identify. When the suction is completed, that is, the second identification device 32 triggers the second signal, the control device controls the suction device 2 to stop suction and controls the suction device 2 to move away from the first centrifugal cavity, At this time, the centrifugal device 1 is rotated by 180°, and the second centrifugal chamber is aligned with the suction device 2, and the suction device 2 performs suction. At this time, the first centrifuge chamber corresponds to the supply device 4, and the resupply device 4 adds physiological saline into the first centrifuge chamber, and leaves it still to obtain a suspension of red blood cells, but the suspension of red blood cells at this time is not pure, so physiological saline needs to be added. brine for washing. Rotate the centrifuge device 1 after the suction of the second centrifuge chamber is completed, add physiological saline in the second centrifuge chamber and centrifuge the first and second centrifuge cavities again. red blood cell suspension.

In this application, after the preparation of the red blood cell suspension is completed, in order to ensure the service life of the red blood cell automatic production device and prevent environmental pollution, it is necessary to inactivate the waste liquid at high temperature and wash the device. Therefore, the suction device 2 The waste liquid inside is discharged after high-temperature inactivation, and then the centrifugal chamber 11a is filled with physiological saline through the replenishment device 4 to wash the centrifugal chamber 11a, and the physiological saline in the centrifugal chamber 11a is drained through the suction device 2 Suction is performed to wash the suction device 2, and finally the heating device 5 is used for heat sterilization to obtain a clean and sterile centrifuge chamber 11a for the next production of red blood cell suspension.

The above are only optional embodiments of the present application, and are not intended to limit the patent scope of the present application. Under the inventive concept of the present application, the equivalent structural transformations made by using the description of the application and the contents of the accompanying drawings, or direct/indirect Applications in other relevant technical fields are included in the patent protection scope of the present application.

Claims (10)

1. An automatic production device for red blood cell suspension, comprising:

   The centrifugal device includes a reaction box, a driving device and a reaction bottle. A reaction chamber is formed in the reaction box, and the reaction bottle is rotatably arranged in the reaction chamber along the axis of the height direction of the reaction box. The reaction bottle A centrifugal cavity is formed inside, and the driving device drives the reaction bottle to rotate, so as to drive the liquid in the centrifugal cavity to perform centrifugal motion for stratification;

   A suction device, including a suction part movably installed on the reaction box in the direction of approaching and away from the centrifugal cavity, and a driving part for driving the suction part, so as to drive the The suction part is close to the centrifugal cavity to suck the liquid in the centrifugal cavity;

   A supply device, configured to add physiological saline into the centrifuge chamber;

   The identification device includes a first identification device provided on the suction part and a second identification device provided at the bottom of the centrifugal chamber, the first identification device senses that the liquid in the centrifugal chamber has completed centrifugation The first signal is triggered after the first layer, and the second identification device triggers the second signal after sensing that the suction part performs suction; and,

   a control device, electrically connected to the centrifugal device, the suction device, the replenishment device and the identification device, so as to drive the suction device close to the centrifugal cavity when receiving the first signal , and when the second signal is received, the suction device is stopped and the replenishment device is driven to add physiological saline to the centrifuge cavity.
2. The device for automatically producing red blood cell suspension according to claim 1, wherein the suction device comprises a movable sleeved fixed cylinder and a movable cylinder, and the movable cylinder has a movable stroke close to or away from the centrifugal chamber ;

   The suction device also includes a first piston rod, an air pump, and an accumulator, and the first piston rod is movably arranged in the fixed cylinder to separate the fixed cylinder into a section close to the centrifugal cavity. The first cavity segment and the second cavity segment away from the centrifugal cavity; the air pump supplies air to the second cavity segment to drive the first piston rod to move close to the centrifugal cavity, and the accumulator supplies energy to the centrifugal cavity. supply air to the first chamber section to drive the first piston rod to move away from the centrifugal chamber;

   Wherein, the first piston rod is connected to an end of the movable cylinder away from the centrifugal chamber.
3. The device for automatically producing red blood cell suspension according to claim 2, wherein two limit switches are arranged at intervals along the direction close to the centrifugal chamber on the fixed cylinder, and the first piston rod is positioned between the two limit switches. The limit switches reciprocate and move in one direction until the limit switch is touched to trigger a signal, and the control device controls the piston rod to move in reverse when receiving the trigger signal.
4. The automatic preparation device for red blood cell suspension according to claim 2, wherein the suction device further comprises:

   The second piston rod is movably arranged in the movable cylinder, so as to separate the movable cylinder into a third cavity segment close to the centrifugal cavity and a fourth cavity segment away from the centrifugal cavity;

   The first drive mechanism is configured to drive the second piston rod to move, the drive mechanism includes a gear and a movable rack, the second piston rod is connected to the rack, and the gear rotates to drive the rack gradually moving toward or away from the centrifuge chamber to move the second piston rod toward or away from the centrifuge chamber; and,

   A needle tube communicated with the fourth chamber segment, and one end extends toward the direction of the centrifuge chamber to extend out of the fourth chamber segment;

   Wherein, the first identification device is arranged at one end of the fourth chamber segment close to the centrifugal chamber.
5. The device for automatically producing red blood cell suspension according to claim 4, wherein a thermal resistance wire is provided on the inner wall of the fourth chamber section, and is configured to heat the liquid in the fourth chamber section.
6. The device for automatically producing red blood cell suspension according to claim 4, wherein, the first cavity is provided with an air inlet channel, and the air inlet channel is provided with a first control valve; and/or,

   A first passage is provided between the first cavity and the air pump, and a second control valve is provided on the first passage; and/or,

   A second channel is provided between the second cavity and the accumulator, and a third control valve is provided on the second channel; and/or,

   A liquid outlet channel is provided on the third cavity, and a fourth control valve is provided on the liquid outlet channel; and/or,

   The needle tube is provided with a fifth control valve.
7. The automatic preparation device for red blood cell suspension according to claim 1, wherein a plurality of reaction bottles are arranged in the reaction chamber, and each reaction bottle includes a bottle body and a bottle cap, and the bottle cap is configured to seal the described bottle body, the centrifugal device also includes a link mechanism and a drive motor, each of the bottle caps is connected to the drive motor through a link mechanism, and the drive motor drives the link mechanism to move, so that each The bottle cap is movable to open and close the bottle body.
8. The automatic preparation device for red blood cell suspension according to claim 1, wherein the replenishment device comprises:

   A liquid storage tank is movably arranged on the top of the centrifugal chamber along a direction close to or away from the centrifugal chamber, and is configured to hold physiological saline;

   a pipe configured to communicate with the liquid storage tank and the centrifuge chamber; and,

   The second driving mechanism is arranged outside the liquid storage tank and is configured to drive the liquid storage tank to move close to or away from the centrifugal chamber, and the liquid storage tank moves to realize the connection between the liquid storage tank and the centrifugal chamber. The liquid level difference of the liquid changes, so that the liquid in the liquid storage tank is input into the centrifugal chamber through the pipeline.
9. The automatic preparation device for red blood cell suspension according to claim 1, wherein, the automatic preparation device for red blood cell suspension further comprises a heating device, and the heating device is arranged on the outside of the reaction bottle, and is set to the inside of the centrifuge chamber. liquid is heated.
10. A manufacturing method based on the red blood cell suspension automatic manufacturing device described in claims 1-9, comprising the following steps:

    controlling the centrifugal device to perform centrifugal stratification on the centrifuge cavity filled with anticoagulated whole blood;

    receiving the first signal sent by the first identification device, controlling the suction device to suck the upper liquid level in the centrifuge chamber;

    receiving the second signal sent by the second identification device, controlling the suction device to stop suction, and controlling the replenishment device to inject physiological saline into the centrifuge cavity to obtain a suspension of red blood cells;

    After the red blood cell suspension in the centrifuge cavity is taken out, the replenishment device is controlled to fill the centrifuge cavity with physiological saline;

    The suction device is controlled to suck the physiological saline in the centrifugal cavity, so as to discharge the physiological saline in the suction device.