LU502241B1 - Automatic production device and method for producing red cell suspension - Google Patents
Automatic production device and method for producing red cell suspension Download PDFInfo
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- LU502241B1 LU502241B1 LU502241A LU502241A LU502241B1 LU 502241 B1 LU502241 B1 LU 502241B1 LU 502241 A LU502241 A LU 502241A LU 502241 A LU502241 A LU 502241A LU 502241 B1 LU502241 B1 LU 502241B1
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- centrifugal
- centrifugal cavity
- cell suspension
- liquid
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- 239000006285 cell suspension Substances 0.000 title claims abstract description 63
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 56
- 239000007788 liquid Substances 0.000 claims abstract description 97
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 39
- 230000007246 mechanism Effects 0.000 claims description 29
- 238000003860 storage Methods 0.000 claims description 29
- 210000004369 blood Anatomy 0.000 claims description 21
- 239000008280 blood Substances 0.000 claims description 21
- 238000010438 heat treatment Methods 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 239000003146 anticoagulant agent Substances 0.000 claims description 4
- 229940127219 anticoagulant drug Drugs 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 4
- 210000003743 erythrocyte Anatomy 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 239000000523 sample Substances 0.000 description 7
- 230000001960 triggered effect Effects 0.000 description 6
- 239000002699 waste material Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 206010034719 Personality change Diseases 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- External Artificial Organs (AREA)
- Centrifugal Separators (AREA)
Abstract
The present application discloses an automatic production device and method for producing red cell suspension, the automatic production device includes a centrifugal device, a suction device, a supply device, an identification device and a control device for controlling the automatic production device, the identification device including a first identification device provided in the suction device and a second identification device provided in the centrifugal cavity; the driving device driving the centrifugal cavity to carry out centrifugal movement to make the liquid in the centrifugal cavity centrifuge for layering; the first identification device identifing and triggering a first signal to the control device; the control device controlling the suction device to suck the layered liquid; after the second identification device triggers the second signal to the control device, the control device controlling the suction device to stop and controlling the supply device to add normal saline into the centrifugal cavity. The present realizes the automatic production for producing red blood cell suspension through the identification of the identification device and the control of the control device, thereby improving the production efficiency and avoiding wasting time.
Description
AUTOMATIC PRODUCTION DEVICE AND METHOD FOR PRODUCING RED CELL
SUSPENSION 50220
[0001] The present application claims the priority to Chinese patent application No. 202111461594.2, titled “Automatic production device and method for producing red cell suspension” and filed on December 2, 2021, which is hereby incorporated for reference in its entirety for all purposes.
[0002] The present application relates to the technical field of red cell suspension production, in particular to an automatic production device for producing red cell suspension and an automatic production method for producing red cell suspension.
[0003] In the production of the red cell suspension, first a whole blood centrifugation is performed on anticoagulant venous blood, normal saline is added after centrifugation and mix well, the upper live liquid is removed, and then the centrifugation is performed again, those steps are repeated until the upper live liquid after centrifugation is transparent to obtain overstocked red cells. Then, one drop of overstocked red cells and nine drops of normal saline are mixed to obtain 10% red cell suspension.
[0004] However, this production process of the red cell suspension involves many key operations, such as adding blood samples, adding normal saline, centrifuging blood samples, and extracting overstocked red cells. At present, these operations are mostly realized by manual operation. Repeated operations are easy to reduce the operation accuracy and increase fatigue, which is not conducive to mass production. In addition, repeated operations are easy to reduce the configuration efficiency and accuracy, and the red cell suspension is polluted by colonies.
[0005] During the centrifugation of the whole blood to the overstocked red cells, blood samples will contact bacteria in the air multiple times, resulting that the colonies attach the blood samples and grow in the blood samples, and the red cell suspension is polluted. At present, blood samples and normal saline of different concentrations are generally extracted manually by the operator, and centrifugal equipments with different speeds are provided according to different blood samples. Once the demand for suspension configuration increases, the workload of the operator will be greatly increased, and the accuracy is difficult to be maintained in the case of multiple operations. In the process of producing the red cell suspension, the whole blood needs to be centrifuged for many times, and the upper live liquid after centrifugation should be removed. Live liquid (waste plasma, red cells and cell fluid) pollutes the environment and must be inactivated before being discharged into the natural environment. At present, the pipette, the test tube and other auxiliary instruments used in the process of producing the red cell suspension must be cleaned and sterilized after configuring the suspension each time, and then they can be p02 used again. At present, the sterilization is mostly realized by manual cleaning combined with special disinfection equipment. Due to the need for manual transfer of the instruments, the efficiency is low, and it is easy to be stained with bacteria during the transfer.
[0006] The main purpose of the present application is to propose an automatic production device and method for producing red cell suspension, which aims to realize the automation function of multiple key steps of producing red cell suspension, thereby saving the waiting time of manual operation, greatly improving the producing efficiency, and providing technical support for mass production of red cell suspension.
[0007] In order to achieve the above purpose, the present application provides an automatic production device for producing red cell suspension, including:
[0008] a centrifugal device comprising a reaction box, a driving device and a reaction bottle, the reaction box being provided with a reaction cavity, the reaction bottle being rotatably provided in the reaction cavity along an axis in a height direction of the reaction box, a centrifugal cavity being provided in the reaction bottle, the driving device be configured for driving the reaction bottle to rotate to drive liquid in the centrifugal cavity to make centrifugal movement for layering;
[0009] a suction device comprising a suction part mounted in the reaction box to be close to or away from the centrifugal cavity, and a driving part for driving the suction part to move close to the centrifugal cavity to suck the liquid in the centrifugal cavity;
[0010] a supply device for adding normal saline to the centrifugal cavity;
[0011] an identification device comprising a first identification device provided at the suction part and a second identification device provided at a bottom of the centrifugal cavity, the first identification device being configured for triggering a first signal after detecting completion of centrifugal layering of the liquid in the centrifugal cavity, and the second identification device being configured for triggering a second signal after detecting that the suction part is sucking; and,
[0012] a control device electrically connected with the centrifugal device, the suction device, the supply device and the identification device for driving the suction device to be close to the centrifugal cavity upon receiving the first signal, stopping the suction device upon receiving the second signal, and driving the supply device to add normal saline into the centrifugal cavity.
[0013] Optionally, the suction device includes a movable cylinder and a fixed cylinder sleeved on the movable cylinder, and the movable cylinder is movable close to or away from the centrifugal cavity;
[0014] the suction device further comprises a first piston rod, an air pump and an 06661 accumulator, the first piston rod is movably provided in the fixed cylinder to divide the fixed cylinder into a first cavity section close to the centrifugal cavity and a second cavity section away from the centrifugal cavity, the air pump is configured for supplying air to the second cavity section to drive the first piston rod to move close to the centrifugal cavity, and the accumulator is configured for supplying air to the first cavity section to drive the first piston rod to move away from the centrifugal cavity; and
[0015] wherein the first piston rod is connected with one end of the movable cylinder away from the centrifugal cavity.
[0016] Optionally, two limit switches are provided on the fixed cylinder at intervals along a direction close to the centrifugal cavity, the first piston rod is movable back and forth between the two limit switches, and a trigger signal is sent when the first piston rod moves to touch a limit switch in one direction, the control device is configured for controlling the first piston rod to move in an opposite direction upon receiving the trigger signal.
[0017] Optionally, the suction device further includes:
[0018] a second piston rod movably provided in the movable cylinder to divide the movable cylinder into a third cavity section close to the centrifugal cavity and a fourth cavity section away from the centrifugal cavity;
[0019] a first driving mechanism for driving the second piston rod and comprising a gear and a movable rack, the second piston rod being connected with the rack, the gear being configured for rotating to drive the rack to move gradually close to or away from the centrifugal cavity and make the second piston rod move close to or away from the centrifugal cavity; and,
[0020] a needle tube communicated with the fourth cavity section, one end of the needle tube extending towards the centrifugal cavity and extending out of the fourth cavity section;
[0021] wherein the first identification device is provided at one end of the fourth cavity section close to the centrifugal cavity.
[0022] Optionally, a thermal resistance wire is provided on an inner wall of the fourth cavity section for heating the liquid in the fourth cavity section.
[0023] Optionally, the first cavity section is provided with an air inlet channel, and the air inlet channel is provided with a first control valve; and/or,
[0024] a first channel is provided between the first cavity section and the air pump, and a second control valve is provided in the first channel; and/or,
[0025] a second channel is provided between the second cavity section and the accumulator, and a third control valve is provided in the second channel; and/or,
[0026] the third cavity section is provided with a liquid outlet channel, and the liquid outlet channel is provided with a fourth control valve; and/or, 1006661
[0027] the needle tube is provided with a fifth control valve.
[0028] Optionally, a plurality of reaction bottles are provided in the reaction cavity, each reaction bottle comprises a bottle body and a bottle cap for sealing the bottle body, the centrifugal device further comprises a connecting rod mechanism and a driving motor, the bottle cap is connected with the driving motor through the connecting rod mechanism, and the driving motor is configured to drive the connecting rod mechanism to move and make the bottle cap to move to open and close the bottle body.
[0029] Optionally, the supply device includes:
[0030] a liquid storage box movably provided on a top of the centrifugal cavity along a direction close to or away from the centrifugal cavity for holding normal saline;
[0031] a pipe for communicating the liquid storage box and the centrifugal cavity; and,
[0032] a second driving mechanism provided outside of the liquid storage box for driving the liquid storage box to move close to or away from the centrifugal cavity, the liquid storage box moving to change a liquid level difference between liquid in the liquid storage box and the liquid in the centrifugal cavity and input the liquid in the liquid storage box into the centrifugal cavity through the pipe.
[0033] Optionally, further including a heating device provided on an outer side of the reaction bottle for heating the liquid in the centrifugal cavity.
[0034] Correspondingly, the present application provides a producing method based on the automatic production device for producing red cell suspension, including:
[0035] controlling the centrifugal device to centrifuge anticoagulant venous blood in the centrifugal cavity for layering;
[0036] controlling the suction device to suck the upper liquid in the centrifugal cavity upon receiving the first signal sent by the first identification device;
[0037] controlling the suction device to stop sucking upon receivingthe second signal sent by the second identification device, and controlling the supply device to supply normal saline into the centrifugal cavity to obtain the red cell suspension;
[0038] controlling the supply device to fill the centrifugal cavity with the normal saline after the red cell suspension in the centrifugal cavity is taken out; and
[0039] controlling the suction device to suck the normal saline in the centrifugal cavity and discharge the normal saline.
[0040] In the technical solution of the present application, the control device controls the driving device to drive the reaction bottle to rotate to layer the liquid in the centrifugal cavity.
After receiving the first signal triggered by the first identification device, the control device controls the suction part to suck the liquid in the centrifugal cavity. After receiving the second p02 signal triggered by the second identification device, the control device controls the suction device to stop and controls the supply device to add normal saline into the centrifugal cavity to 5 obtain red cell suspension. The identification device transmits signals to the control device for automatic control to complete the automatic production of red cell suspension, such that it realizes the automation function of multiple key steps in the production of red cell suspension, thereby saving the waiting time of manual operation, greatly improving the production efficiency, and providing technical support for the mass production of red cell suspension.
[0041] In order to more clearly explain the embodiments of the present application or the technical solutions in the related art, the following will briefly introduce the drawings in the embodiments or the description of the related art. It is obvious that the drawings in the following description are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained according to the structure shown in these drawings without paying creative labor.
[0042] Fig. 1 is a schematic diagram of an automatic production device for producing red cell suspension according to the present application.
[0043] Fig. 2 is a structural diagram of a centrifugal device in Fig. 1.
[0044] Fig. 3 is a structure of a suction device in Fig. 1.
[0045] Fig. 4 is a structural diagram of a supply device in Fig. 1.
[0046] Description of attached drawing label: 100 Automatic production device 223 Needle tube for producing red cell suspension
213 Accumulator 31 First identification pm Te 214 First cavity section 32 Second identification me TE 221 Second piston rod 43 Second driving
CT mi
[0047] The realization of the purpose, functional features and advantages of the present application will be further described with reference to the attached drawings in combination with the embodiments.
[0048] The technical solution in the embodiment of the present application will be clearly and completely described below in combination with the attached drawings in the embodiment of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work belong to the claimed scope of the present application.
[0049] It should be noted that if the embodiment of the present application involves directional indication (such as up, down, left, right, front, back...), the directional indication is only used to explain the relative position relationship and movement among components in a specific attitude (as shown in the attached drawings). If the specific attitude changes, the directional indications will change accordingly.
[0050] In addition, there is the description of “first”, “second” and the like in the present invention, the description of “first”, “second” and the like is only for descriptive purposes, and cannot be understood as indicating or implying its relative importance or implicitly indicating the number of indicated technical features. Thus, the features defined with “first” and “second” may include at least one of the features explicitly or implicitly. In addition, the meaning of “and/or” in the full text includes three parallel schemes. Taking “A and/or B” as an example, it includes scheme A, or scheme B, or both scheme A and B. In addition, the technical solutions of various embodiments can be combined with each other, but it must be based on the realization of those skilled in the art. When the combination of technical solutions is contradictory or impossible, it should be considered that the combination of technical solutions does not exist and is not within the claimed scope of the present application. 502247
[0051] The production process of the existing red cell suspension needs to involve many key steps, such as adding blood sample, adding normal saline, centrifuging blood sample, and extracting overstocked red cells. At present, these operation steps are mostly realized by manual operation. Repeated operation for many times is easy to reduce the operation accuracy and increase fatigue, which is not conducive to mass production. There is an urgent need for an automatic and intelligent device to realize the integrated production of red cell suspension. In view of this, the present application provides an automatic production device for producing red cell suspension, saving the time of manual operation, greatly improving the producing efficiency, and providing technical support for the mass production of red cell suspension.
[0052] Referring to Figs. 1 to 4, the present application provides an embodiment of an automatic production device for producing red cell suspension.
[0053] Referring to Fig. 1 to Fig. 2, an automatic production device 100 for producing red cell suspension includes a centrifugal device 1, a suction device 2, a supply device 4, an identification device 3 and a control device. The centrifugal device 1 includes a reaction box, a driving device and a reaction bottle 11. The reaction box is provided with a reaction cavity. The reaction bottle 11 is rotatably provided in the reaction cavity along an axis in a height direction of the reaction box, and a centrifugal cavity 11a is provided in the reaction bottle 11. The driving device drives the reaction bottle 11 to rotate to drive the liquid in the centrifugal cavity 11a to make centrifugal movement for layering. The suction device 2 includes a suction part mounted in the reaction box in a direction close to and away from the centrifugal cavity 11a, and a driving part for driving the suction part. The suction part is driven by the driving part to be close to the centrifugal cavity 11a to suck the liquid in the centrifugal cavity 11a. The supply device 4 is configured for adding normal saline to the centrifugal cavity 11a. The identification device 3 includes a first identification device 31 provided at the suction part and a second identification device 32 provided at a bottom of the centrifugal cavity 11a. The first identification device 31 triggers a first signal after detecting completion of centrifugal layering of the liquid in the centrifugal cavity 1la, and the second identification device 32 triggers a second signal after detecting that the suction part is sucking. The control device is electrically connected with the centrifugal device 1, the suction device 2, the supply device 4 and the identification device 3 to drive the suction device 2 to be close to the centrifugal cavity 11a upon receiving the first signal, and to stop the suction device 2 upon receiving the second signal and drive the supply device 4 to add normal saline into the centrifugal cavity 11a.
[0054] In the embodiment, the control device controls the driving device to drive the reaction bottle 11 to rotate to layer the liquid in the centrifugal cavity 11a. Upon receiving the first signal triggered by the first identification device 31, the control device controls the suction part to suck 22H! the liquid in the centrifugal cavity 11a. Upon receiving the second signal triggered by the second identification device 32, the control device controls the suction device 2 to stop and controls the supply device 4 to add normal saline into the centrifugal cavity 11a to obtain red cell suspension.
The identification device 3 transmits signals to the control device for automatic control and completing the automatic production of red cell suspension. The automation of multiple key steps in the production of red cell suspension is realized, thereby saving the waiting time of manual operation, greatly improving the production efficiency, and providing technical support for the mass production of red cell suspension.
[0055] It is worth mentioning that the first identification device 31 and the second identification device 32 each includes a photoelectric liquid level switch. The photoelectric liquid level switch includes a light-emitting element and a receiving element. The light-emitting element and the receiving element can be a light-emitting crystal and a receiving crystal respectively, or other elements that can emit or receive light, which are not limited here. Based on the principle of reflection and refraction of light at an interface of two different media, the light-emitting element detects an intensity difference between light emitted by the light-emitting element and light reflected back and determines whether a preset condition is met. The specific principle is that the first identification device 31 continuously detects an upper surface of the whole blood in the centrifugal cavity 11a, and the light-emitting element emits light. At this time, an intensity of the reflected light received by the receiving element is Q1. When the suction part of the suction device 2 contacts the upper surface of the whole blood, an intensity of the reflected light received by the receiving element is Q2. When the received intensity changes from Q1 to
Q2, it is equivalent to that the first signal is generated, the control device starts to control the suction part for suction. The second identification device 32 continuously detects the layered liquid in the centrifugal cavity 11a, and the light-emitting element emits light. When there are two layers of layered liquid, an intensity of the reflected light received by the receiving element is Q3. When only one layer of liquid is detected, an intensity of the reflected light received by the receiving element is Q4. When the received intensity changes from Q3 to Q4, it is equivalent to that the second signal is generated. The control device controls the suction part to stop suction and the supply device to supply normal saline.
[0056] The first identification device 31 identifies a refractive light difference of the light in the air and in the liquid level, and then transmits a signal to the control device. The second identification device 32 identifies a refractive light difference of the light in different liquid level, and then transmits a signal to the control device, so as to control the suction part to suck or stop sucking. During centrifugation, the centrifugal cavity 11a is in a closed state to prevent the liquid to be centrifuged from flying out of the centrifugal cavity 1la during centrifugation. During 06661 suction and supply, the reaction cavity is also in the closed state to avoid the situation that the blood sample and bacteria in the air contact and result in that the colonies attach the blood sample and grow in the blood sample and the red cell suspension is polluted.
[0057] Referring to Fig. 3, the suction device 2 includes a movable cylinder 22 and a fixed cylinder 22 sleeved on the movable cyclinder 22, the movable cylinder 22 is movable close to or away from the centrifugal cavity 11a. The suction device 2 also includes a first piston rod 211, an air pump 212 and an accumulator 213. The first piston rod 211 is movably provided in the fixed cylinder 21 to divide the fixed cylinder 21 into a first cavity section 214 close to the centrifugal cavity 11a and a second cavity section 215 away from the centrifugal cavity 11a. The air pump 212 supplies air to the second cavity section 215 to drive the first piston rod 211 to move close to the centrifugal cavity 11a, and the accumulator 213 supplies air to the first cavity section 214 to drive the first piston rod 211 to move away from the centrifugal cavity 11a. The first piston rod 211 is connected with one end of the movable cylinder 22 away from the centrifugal cavity 11a.
[0058] In this embodiment, the air pump 212 makes the first piston rod 211 to move close to the centrifugal cavity 11a, and the air in the air pump 212 will enter the accumulator 213 for storage after driving the first piston rod 211 to move. When the first piston rod 211 moves to a preset position, the accumulator 213 drives the first piston rod 211 to move away from the centrifugal cavity 11a. The air pump 212 and the accumulator 213 can not only protect the environment, but also save resources. The fixed cylinder 21 is sleeved on the movable cylinder 22. The fixed cylinder 21 is connected with the movable cylinder 22 through the first piston rod 211. The first piston rod 211 pushes the movable cylinder 22 to be close to the liquid level for suction, and the suction part is formed in the movable cylinder 22. The separated two sections of the cylinder is convenient not only for control, but also for disassembly, cleaning and replacement.
[0059] Further, the fixed cylinder 21 is provided with two limit switches 216 at intervals along a direction close to the centrifugal cavity 11a, the first piston rod 211 moves back and forth between the two limit switches 216, and a trigger signal is sent when the first piston rod 211 moves to touch the limit switch 216 in one direction. The control device controls the first piston rod to move in an opposite direction when receiving the trigger signal.
[0060] In this embodiment, a limit switch 216 is provided on the fixed cylinder 21, and the positions of the two limit switches 216 can be adjusted according to the actual situation to make the movable stroke of the first piston rod 211 controllable. When the suction part is to suck, the first piston rod 211 moves close to the centrifugal cavity 1la for suction. During suction,
according to the signal transmitted by the first identification device 31, the control device controls the first piston rod 211 to move gradually close to the centrifugal cavity 11a. When the 06661 piston rod 211 reaches the limit switch close to the centrifugal cavity 11a, the control device controls the first piston rod 211 to move away from the centrifugal cavity 11a. When the first piston rod 211 reaches the limit switch away from the centrifugal cavity 11a, the control device determines whether to continue to control the first piston rod 211 to move in the opposite direction. When receiving the signal triggered by the limit switch, the control device controls the first piston rod to move in the opposite direction for intelligent control, such that the blood sample is prevented from contacting with the air to pollute the red cell suspension during control.
[0061] Referring to Fig. 3, the suction device 2 also includes a second piston rod 221, a first driving mechanism 222 and a needle tube 223. The second piston rod 221 is movably provided in the movable cylinder 22 to divide the movable cylinder 22 into a third cavity section 224 close to the centrifugal cavity 11a and a fourth cavity section 225 away from the centrifugal cavity 11a.
The first driving mechanism 222 is configured to drive the second piston rod 221 to move, the driving mechanism includes a gear and a movable rack, the second piston rod 221 is connected with the rack, and the gear rotates to drive the rack to move gradually close to or away from the centrifugal cavity 1la to make the second piston rod 221 to move close to or away from the centrifugal cavity 11a. The needle tube 223 is communicated with the fourth cavity section 225, and one end of the needle tube 223 is extended towards the centrifugal cavity 11a and out of the fourth cavity section 225. The first identification device 31 is provided at one end of the fourth cavity section 225 close to the centrifugal cavity 11a.
[0062] In this embodiment, the whole movable cylinder 22 is used as a needle to suck the upper liquid level after layering. The second piston rod 221 can be movably provided in the movable cylinder 22, and the movable cylinder 22 is divided into the third cavity section 224 and the fourth cavity section 225. During suction, the first driving mechanism 222 drives the first piston rod 211 to move close to the centrifugal cavity 11a first. At this time, a volume of the third cavity section 224 is similar to a volume of the whole movable cylinder 22. Then the first driving mechanism 222 drives the first piston rod 211 to move away from the centrifugal cavity 11a to form a negative pressure at the fourth cavity section 225 and the needle tube 223 to suck the liquid in the centrifugal cavity 11a. The first driving mechanism 222 includes a gear and a rack.
The gear and the rack mesh for controlling a sucking amount, which facilitates more accurate suction and prevents excessive suction. The first driving mechanism can also include two gears meshing with each other, which can accurately control the movement of the second piston rod 221, and is not limited here. The first identification device 31 is provided at one end of the fourth cavity section 225 close to the centrifugal cavity 1la to facilitate an identification of the first identification device 31.
[0063] Further, an inner wall of the fourth cavity section 225 is provided with a Le resistance wire 226 for heating the liquid in the fourth cavity section 225.
[0064] In this embodiment, an inner wall of the fourth cavity section 225 is provided with a thermal resistance wire 226. The thermal resistance wire 226 after being powered on can inactivate the waste liquid in the fourth cavity section 225 at high temperature, and avoid to pollute the environment when the waste liquid is discharged. At the same time, the thermal resistance wire 226 can also conduct high-temperature treatment in the fourth cavity section 225 before suction to purify the environment in the fourth cavity section 225. In the present application, the device for high-temperature inactivation can also be other devices which can realize heating inactivation effect, such as an infrared heating device, and thus 1s not limited to the thermal resistance wire 226.
[0065] Referring to Fig. 3, the first cavity section is provided with an air inlet channel, and the air inlet channel is provided with a first control valve 23; and/or, a first channel is provided between the first cavity section and the air pump 212, and a second control valve 24 1s provided on the first channel; and/or, a second channel 1s provided between the second cavity section and the accumulator 213, and a third control valve 25 is provided on the second channel; and/or, the third cavity section is provided with a liquid outlet channel, and the liquid outlet channel is provided with a fourth control valve 26; and/or, the needle tube 223 1s provided with a fifth control valve 27.
[0066] In this embodiment, the first control valve 23 is configured to seal the first cavity section 214, so that the air pump 212 and the accumulator 213 drive the first piston rod 211 to move. After the second control valve 24 is opened, the air pump 212 supplies air to the first cavity section 214, and after the third control valve 25 1s opened, the accumulator 213 supplies air to the second cavity section 215. In order to facilitate the discharge of the liquid in the second cylinder. A liquid outlet channel communicated with the outside is provided on the second cylinder. The fourth control valve 26 controls the liquid outlet channel to be opened or closed, and the fifth control valve 27 is provided on the needle tube 223 to discharge or prohibit to discharge the liquid in the needle tube 223. A plurality of control valves can make the control of the suction device 2 more convenient during suction, and meet the needs of more suction situations, and make the automatic suction device 100 for producing red cell suspension more intelligent. The plurality of control valves are electrically connected with the control device to facilitate the control thereof. The specific control mode is that the first identification device 31 starts to identify, and triggers the first signal, then the second control valve 23 is opened, the third control valve 25 is closed, and the first piston rod 211 moves close to the centrifugal cavity
11a. After the second signal is triggered, the first control valve 23 is closed, the second control valve 24 is closed, the third control valve 25 is opened, and the first piston rod 211 moves away oo from the centrifugal cavity 1la. It is worth mentioning that the specific forms of the control valves are not limited. The control valves can be solenoid valves or other electric valves.
[0067] Referring to Fig. 1 and Fig. 2, a plurality of reaction bottles 11 are provided in the reaction cavity. Each reaction bottle 11 includes a bottle body and a bottle cap for sealing the bottle body. The centrifugal device 1 also includes a connecting rod mechanism 12 and a driving motor 13. Each bottle cap is connected with the driving motor 13 through the connecting rod mechanism 12, and the driving motor 13 drives the connecting rod mechanism 12 to move, and make each bottle cap to move to open or close the bottle body.
[0068] In this embodiment, the reaction cavity is cylindrical, and two opposite reaction bottles 11 are provided in the reaction cavity along a radial direction of the reaction cavity. The corresponding two reaction bottles 11 can make the objects in the centrifugal device 1 more stable during the centrifugal movement. In the present application, the number of reaction bottles 11 in the reaction cavity is even and the reaction bottles are symmetrically provided in the reaction cavity, which is convenient to improve the stability of the objects in the centrifugal cavity 11a. Meanwhile, in this embodiment, it is a way to use a bottle cap to seal a bottle body, and use the driving motor 13 and the connecting rod mechanism 12 as a mechanism to drive the bottle cap to seal the bottle body. Both are used to seal the bottle body together. In other embodiments, detachable sealing bags, etc. can be used to seal. Here, the way to seal the reaction bottles 11 is not specifically limited.
[0069] Referring to Fig. 4, the supply device 4 includes a liquid storage box 41, a pipe 42 and a second driving mechanism 43. The liquid storage box 41 is movably provided on a top of the centrifugal cavity 1la in a direction close to or away from the centrifugal cavity 1la for holding normal saline. The pipe 42 is used to communicate the liquid storage box 41 with the centrifugal cavity 11a. The second driving mechanism 43 is provided outside of the liquid storage box 41 to drive the liquid storage box 41 to move close to or away from the centrifugal cavity 11a. The liquid storage box 41 moves to change the liquid level difference between the liquid in the liquid storage box 41 and the liquid in the centrifugal cavity 11a, and input the liquid in the liquid storage box 41 into the centrifugal cavity 11a through the pipe 42.
[0070] In this embodiment, the liquid in the liquid storage box 41 is input into the reaction cavity by using the siphon principle, the pipe 42 is a U-shaped siphon, the second driving mechanism 43 drives the liquid storage box 41 to move, and the liquid in the liquid storage box 41 is transmitted to the centrifugal cavity 11a by the liquid level difference. Since the amount of normal saline required for the production of red cell suspension is mostly in ml, it is necessary to carry out accurate micro feeding. Therefore, the second driving mechanism 43 is a gear and rack mechanism, the rack is connected with the liquid storage box 41. The gear drives the rack o p02 move close to or away from the centrifugal cavity 11a, so as to drive the liquid storage box 41 to move. According to the feed rate of each tooth of the gear, the cross-section area of the liquid storage box 41 and the normal saline to be added, the number of teeth to be rotated of the gear is calculated, and the accuracy of the amount of normal saline to be added is ensured through the accurately calculated feed rate. In the present application, the specific form of the second driving mechanism 43 is not limited. It can also be driven by the meshing of two gears or driven by threaded screws, which can ensure the accurate feeding of normal saline.
[0071] Referring to Figs. 1 and 2, the automatic production device 100 for producing red cell suspension also includes a heating device 5 provided on an outer side of the reaction bottle 11 for heating the liquid in the centrifugal cavity 11a.
[0072] In this embodiment, the heating device 5 is a thermal resistance wire 226 for inactivating or disinfecting specific substances in the centrifugal cavity 11a at high temperature.
Inthe present invention, the specific form of the heating device 5 is not limited, but can be a pipe heater, an infrared heating, or the like.
[0073] The present application also proposes a producing method for producing red cell suspension based on the automatic production device 100 for producing red cell suspension, the producing method includes:
[0074] controlling a centrifugal device 1 to centrifuge anticoagulant venous blood in a centrifugal cavity 11a for layering;
[0075] controlling a suction device 2 to suck upper liquid in the centrifugal cavity 11a when receiving a first signal sent by a first identification device 31;
[0076] controlling the suction device 2 to stop sucking upon receiving a second signal sent by a second identification device 32, and controlling a supply device 4 to supply normal saline into the centrifugal cavity 11a to obtain red cell suspension;
[0077] controlling the supply device 4 to fully fill the centrifugal cavity 11a with the normal saline after the red cell suspension in the centrifugal cavity 11a is taken out; and
[0078] controlling the suction device 2 to suck the normal saline in the centrifugal cavity 11a to discharge the normal saline in the suction device 2.
[0079] Specifically, in this embodiment, the centrifugal device 1 includes a cylindrical cavity, two reaction bottles 11 are provided in the cavity along both sides of the central axis of the cavity (the reaction bottle 11 is provided with a centrifugal cavity 11a, hereinafter referred to as a first centrifugal cavity and a second centrifugal cavity), the reaction bottle 11 includes a bottle body and a bottle cap for sealing the bottle body, and the connecting rod mechanism 12 is connected with the bottle caps of the two reaction bottles 11. The driving motor 13 drives the bottle cap to seal or contact to seal the bottle body. After centrifugal layering, the suction device 2 is close o p02 the first centrifugal cavity, and the first identification device 31 starts to identify. When the first signal is received, the suction part starts to suck. At this time, the second identification device 32 starts to identify. When the suction is completed, that is, the second identification device 32 triggers the second signal. The control device controls the suction device 2 to stop sucking and controls the suction device 2 to be away from the first centrifugal cavity. At this time, the centrifugal device 1 rotates 180°, the second centrifugal cavity is aligned with the suction device 2, and the suction device 2 performs suction. At this time, the first centrifugal cavity corresponds to the supply device 4. The supply device 4 adds normal saline into the first centrifugal cavity and then the liquid in the first centrifugal cavity stands to obtain the red cell suspension.
However, the red cell suspension is not pure at this time, so it needs to add normal saline for washing. After the suction of the second centrifugal cavity is completed, the centrifugal device 1 is rotated to add normal saline into the second centrifugal cavity, then the centrifugation is performed in the first centrifugal cavity and the second centrifugal cavity again, and the suction is performed again after centrifugal layering, and finally the pure red cell suspension is obtained.
[0080] In the present application, after the red cell suspension is configured, in order to ensure the service life of the automatic red cell production device and prevent environmental pollution, it is necessary to inactivate the waste liquid at high temperature and wash the device.
Therefore, firstly the waste liquid in the suction device 2 is inactivated at high temperature and then is discharged, and then the centrifugal cavity 11a is fully filled with normal saline through the supply device 4 to wash the centrifugal cavity 11a. The normal saline in the centrifugal cavity 11a is sucked through the suction device 2 to wash the suction device 2, and finally the centrifugal cavity 11a is heated and sterilized by the heating device 5 to obtain a clean and sterile centrifugal cavity 11a for the production of the next red cell suspension.
[0081] The above is only the preferred embodiment of the present application and does not limit the claimed scope of the present application. Under the inventive concept of the present application, the equivalent structural transformation made by using the contents of the description and attached drawings of the present application, or direct/indirect application in other relevant technical fields, is included in the patent claimed scope of the present application.
Claims (10)
1. An automatic production device for producing red cell suspension, comprising: a centrifugal device comprising a reaction box, a driving device and a reaction bottle, the reaction box being provided with a reaction cavity, the reaction bottle being rotatably provided in the reaction cavity along an axis in a height direction of the reaction box, a centrifugal cavity being provided in the reaction bottle, the driving device be configured for driving the reaction bottle to rotate to drive liquid in the centrifugal cavity to make centrifugal movement for layering; a suction device comprising a suction part mounted in the reaction box to be close to or away from the centrifugal cavity, and a driving part for driving the suction part to move close to the centrifugal cavity to suck the liquid in the centrifugal cavity; a supply device for adding normal saline to the centrifugal cavity; an identification device comprising a first identification device provided at the suction part and a second identification device provided at a bottom of the centrifugal cavity, the first identification device being configured for triggering a first signal after detecting completion of centrifugal layering of the liquid in the centrifugal cavity, and the second identification device being configured for triggering a second signal after detecting that the suction part is sucking; and, a control device electrically connected with the centrifugal device, the suction device, the supply device and the identification device for driving the suction device to be close to the centrifugal cavity upon receiving the first signal, stopping the suction device upon receiving the second signal, and driving the supply device to add normal saline into the centrifugal cavity.
2. The automatic production device for producing red cell suspension according to claim 1, wherein the suction device comprises a movable cylinder and a fixed cylinder sleeved on the movable cylinder, and the movable cylinder is movable close to or away from the centrifugal cavity; the suction device further comprises a first piston rod, an air pump and an accumulator, the first piston rod is movably provided in the fixed cylinder to divide the fixed cylinder into a first cavity section close to the centrifugal cavity and a second cavity section away from the centrifugal cavity, the air pump is configured for supplying air to the second cavity section to drive the first piston rod to move close to the centrifugal cavity, and the accumulator is configured for supplying air to the first cavity section to drive the first piston rod to move away from the centrifugal cavity; and wherein the first piston rod is connected with one end of the movable cylinder away from the centrifugal cavity. 1006661
3. The automatic production device for producing red cell suspension according to claim 2, wherein two limit switches are provided on the fixed cylinder at intervals along a direction close to the centrifugal cavity, the first piston rod is movable bac k and forth between the two limit switches, and a trigger signal is sent when the first piston rod moves to touch a limit switch in one direction, the control device is configured for controlling the first piston rod to move in an opposite direction upon receiving the trigger signal.
4. The automatic production device for producing red cell suspension according to claim 2, wherein the suction device further comprises: a second piston rod movably provided in the movable cylinder to divide the movable cylinder into a third cavity section close to the centrifugal cavity and a fourth cavity section away from the centrifugal cavity; a first driving mechanism for driving the second piston rod and comprising a gear and a movable rack, the second piston rod being connected with the rack, the gear being configured for rotating to drive the rack to move gradually close to or away from the centrifugal cavity and make the second piston rod move close to or away from the centrifugal cavity; and, a needle tube communicated with the fourth cavity section, one end of the needle tube extending towards the centrifugal cavity and extending out of the fourth cavity section; wherein the first identification device is provided at one end of the fourth cavity section close to the centrifugal cavity.
5. The automatic production device for producing red cell suspension according to claim 4, wherein a thermal resistance wire is provided on an inner wall of the fourth cavity section for heating the liquid in the fourth cavity section.
6. The automatic production device for producing red cell suspension according to claim 4, wherein the first cavity section is provided with an air inlet channel, and the air inlet channel is provided with a first control valve; and/or, a first channel is provided between the first cavity section and the air pump, and a second control valve is provided in the first channel; and/or, a second channel is provided between the second cavity section and the accumulator, and a third control valve is provided in the second channel; and/or,
the third cavity section is provided with a liquid outlet channel, and the liquid outlet channel is provided with a fourth control valve; and/or, 1006661 the needle tube is provided with a fifth control valve.
7. The automatic production device for producing red cell suspension according to claim 1, wherein a plurality of reaction bottles are provided in the reaction cavity, each reaction bottle comprises a bottle body and a bottle cap for sealing the bottle body, the centrifugal device further comprises a connecting rod mechanism and a driving motor, the bottle cap is connected with the driving motor through the connecting rod mechanism, and the driving motor is configured to drive the connecting rod mechanism to move and make the bottle cap to move to open and close the bottle body.
8. The automatic production device for producing red cell suspension according to claim 1, wherein the supply device comprises: a liquid storage box movably provided on a top of the centrifugal cavity along a direction close to or away from the centrifugal cavity for holding normal saline; a pipe for communicating the liquid storage box and the centrifugal cavity; and, a second driving mechanism provided outside of the liquid storage box for driving the liquid storage box to move close to or away from the centrifugal cavity, the liquid storage box moving to change a liquid level difference between liquid in the liquid storage box and the liquid in the centrifugal cavity and input the liquid in the liquid storage box into the centrifugal cavity through the pipe.
9. The automatic production device for producing red cell suspension according to claim 1, further comprising a heating device provided on an outer side of the reaction bottle for heating the liquid in the centrifugal cavity.
10. A producing method based on the automatic production device for producing red cell suspension according to claims 1-9, comprising: controlling the centrifugal device to centrifuge anticoagulant venous blood in the centrifugal cavity for layering; controlling the suction device to suck the upper liquid in the centrifugal cavity upon receiving the first signal sent by the first identification device; controlling the suction device to stop sucking upon receivingthe second signal sent by the second identification device, and controlling the supply device to supply normal saline into the centrifugal cavity to obtain the red cell suspension; . . . . . . LU502241 controlling the supply device to fill the centrifugal cavity with the normal saline after the red cell suspension in the centrifugal cavity is taken out; and controlling the suction device to suck the normal saline in the centrifugal cavity and discharge the normal saline.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111461594.2A CN114323837B (en) | 2021-12-02 | 2021-12-02 | Automatic manufacturing device and manufacturing method for erythrocyte suspension |
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| Publication Number | Publication Date |
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| LU502241A1 LU502241A1 (en) | 2023-06-02 |
| LU502241B1 true LU502241B1 (en) | 2023-10-09 |
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| LU502241A LU502241B1 (en) | 2021-12-02 | 2022-04-29 | Automatic production device and method for producing red cell suspension |
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| CN (1) | CN114323837B (en) |
| LU (1) | LU502241B1 (en) |
| WO (1) | WO2023097963A1 (en) |
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| CN114323837B (en) * | 2021-12-02 | 2022-10-18 | 湖北文理学院 | Automatic manufacturing device and manufacturing method for erythrocyte suspension |
| CN114703050A (en) * | 2022-04-18 | 2022-07-05 | 湖北文理学院 | Automatic preparation device and preparation method of RNA |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0664052B2 (en) * | 1987-12-24 | 1994-08-22 | 富士写真フイルム株式会社 | Serum suction device |
| US4927545A (en) * | 1988-10-06 | 1990-05-22 | Medical Automation Specialties, Inc. | Method and apparatus for automatic processing and analyzing of blood serum |
| US5439645A (en) * | 1993-01-25 | 1995-08-08 | Coulter Corporation | Apparatus for automatically, selectively handling multiple, randomly associated hematological samples |
| SE513881C2 (en) * | 1994-01-10 | 2000-11-20 | Boule Medical Ab | Method and apparatus for analyzing liquid samples |
| CA2507323A1 (en) * | 2005-05-13 | 2006-11-13 | Chromedx Inc. | Diagnostic whole blood and plasma apparatus |
| KR101587975B1 (en) * | 2013-10-17 | 2016-01-22 | 영동제약 주식회사 | System and Controlling Method Forautomatic Cell Smear |
| US10716880B2 (en) * | 2018-06-15 | 2020-07-21 | Incuvate, Llc | Systems and methods for aspiration and monitoring |
| CN110358673B (en) * | 2019-04-11 | 2022-09-13 | 武汉原生药谷生物医药科技有限公司 | Cell separation system and method |
| CN210030652U (en) * | 2019-04-11 | 2020-02-07 | 武汉原生药谷生物医药科技有限公司 | Tissue cell separation device |
| CN210347276U (en) * | 2019-07-29 | 2020-04-17 | 山东省食品药品检验研究院 | Animal blood preparation device is used in laboratory |
| CN110511863B (en) * | 2019-09-29 | 2024-03-12 | 深圳赛动生物自动化有限公司 | Cell centrifugal split charging device and working method thereof |
| CN210974680U (en) * | 2019-09-29 | 2020-07-10 | 深圳赛动生物自动化有限公司 | Liquid suction mechanism |
| CN110982670A (en) * | 2019-12-27 | 2020-04-10 | 武汉原生药谷生物医药科技有限公司 | Automated cell separation system and method |
| KR102360717B1 (en) * | 2020-02-10 | 2022-02-10 | 주식회사 아이센스 | Method for auto- classifying whole blood/plasma/not-suction using reflected photo sensor |
| CN111205964A (en) * | 2020-03-20 | 2020-05-29 | 中南大学湘雅医院 | Cell separation mechanism and device |
| CN213951155U (en) * | 2020-12-09 | 2021-08-13 | 厦门三一造血技术有限公司 | Portable device for preparing medicinal red blood cells |
| CN114323837B (en) * | 2021-12-02 | 2022-10-18 | 湖北文理学院 | Automatic manufacturing device and manufacturing method for erythrocyte suspension |
-
2021
- 2021-12-02 CN CN202111461594.2A patent/CN114323837B/en active Active
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2022
- 2022-04-29 LU LU502241A patent/LU502241B1/en active IP Right Grant
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| Publication number | Publication date |
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| CN114323837A (en) | 2022-04-12 |
| CN114323837B (en) | 2022-10-18 |
| LU502241A1 (en) | 2023-06-02 |
| WO2023097963A1 (en) | 2023-06-08 |
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