WO2023080304A1 - Antimicrobial degrading agent containing, as active ingredient, mixed strain having antimicrobial activity and organic matter degradation activity, and use thereof - Google Patents
Antimicrobial degrading agent containing, as active ingredient, mixed strain having antimicrobial activity and organic matter degradation activity, and use thereof Download PDFInfo
- Publication number
- WO2023080304A1 WO2023080304A1 PCT/KR2021/016287 KR2021016287W WO2023080304A1 WO 2023080304 A1 WO2023080304 A1 WO 2023080304A1 KR 2021016287 W KR2021016287 W KR 2021016287W WO 2023080304 A1 WO2023080304 A1 WO 2023080304A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- antimicrobial
- organic matter
- antibacterial
- activity
- decomposer
- Prior art date
Links
- 239000005416 organic matter Substances 0.000 title claims abstract description 35
- 230000000845 anti-microbial effect Effects 0.000 title claims abstract description 28
- 239000004480 active ingredient Substances 0.000 title claims abstract description 17
- 230000000694 effects Effects 0.000 title claims abstract description 16
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 6
- 238000006731 degradation reaction Methods 0.000 title abstract description 5
- 230000015556 catabolic process Effects 0.000 title abstract description 4
- 230000000593 degrading effect Effects 0.000 title abstract 3
- 230000000844 anti-bacterial effect Effects 0.000 claims description 46
- 238000000034 method Methods 0.000 claims description 20
- 238000000354 decomposition reaction Methods 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 12
- 241000228197 Aspergillus flavus Species 0.000 claims description 8
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 8
- 244000199866 Lactobacillus casei Species 0.000 claims description 7
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 7
- 241000186684 Lactobacillus pentosus Species 0.000 claims description 7
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 7
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 7
- 244000057717 Streptococcus lactis Species 0.000 claims description 7
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 7
- 229940017800 lactobacillus casei Drugs 0.000 claims description 7
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 7
- 241001277988 Aspergillus sydowii Species 0.000 claims description 6
- 241000194108 Bacillus licheniformis Species 0.000 claims description 6
- 241000266830 Bacillus sonorensis Species 0.000 claims description 6
- 244000063299 Bacillus subtilis Species 0.000 claims description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 6
- 241000187180 Streptomyces sp. Species 0.000 claims description 4
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 1
- 239000008101 lactose Substances 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 8
- 239000004599 antimicrobial Substances 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract 1
- 239000010794 food waste Substances 0.000 description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 15
- 239000001963 growth medium Substances 0.000 description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 241000193830 Bacillus <bacterium> Species 0.000 description 8
- 241000187747 Streptomyces Species 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 239000012153 distilled water Substances 0.000 description 5
- 239000004310 lactic acid Substances 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000006150 trypticase soy agar Substances 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000010800 human waste Substances 0.000 description 3
- 239000010871 livestock manure Substances 0.000 description 3
- 235000019645 odor Nutrition 0.000 description 3
- 239000010801 sewage sludge Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 description 3
- 229960001082 trimethoprim Drugs 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241001629243 Bacillus sonorensis NBRC 101234 = KCTC 13918 Species 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 239000012871 anti-fungal composition Substances 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000010169 landfilling Methods 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229940071440 soy protein isolate Drugs 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B5/00—Operations not covered by a single other subclass or by a single other group in this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/20—Waste processing or separation
Definitions
- the present invention relates to an antibacterial decomposer containing as an active ingredient a mixed strain having antibacterial activity and organic matter decomposition activity and its use.
- the extinction treatment method using such microorganisms has a treatment step of decomposing organic matters of food waste into water and carbon dioxide by the metabolic activity of microorganisms in a device in which the biological environment is appropriately controlled, and volatilizing them into the air.
- Korean Patent No. 0363798 discloses 'a novel strain having organic decomposition and deodorizing function and a food waste treatment method using the same'
- Korean Patent No. 1431250 discloses 'a new strain having antibacterial activity of pathogenic microorganisms.
- a fermented feed additive composition for poultry containing a lactic acid bacterium and a novel useful microorganism having organic matter decomposition activity as active ingredients.
- the 'antibacterial decomposer containing a mixed strain having antibacterial activity and organic matter decomposition activity as an active ingredient and its use' of the present invention has not been disclosed yet.
- the present invention has been derived from the above needs, and the present inventors have prepared an antimicrobial decomposition agent containing as an active ingredient a mixed strain consisting of 4 types of Bacillus bacteria, 4 types of lactic acid bacteria and a strain of the genus Streptomyces, and the antimicrobial decomposition
- the present invention was completed by confirming that the antimicrobial activity and organic matter decomposition activity were excellent.
- the present invention provides an antibacterial decomposer containing a mixed strain having antibacterial activity and organic matter decomposition activity as an active ingredient.
- the present invention provides an antibacterial composition containing the antimicrobial decomposer as an active ingredient.
- the present invention provides a composition for decomposing organic matter containing the antimicrobial decomposer as an active ingredient.
- the present invention provides a method of decomposing organic matter comprising the step of treating the organic matter with the antimicrobial decomposer.
- the antibacterial decomposer of the present invention has excellent antibacterial activity and organic matter decomposition activity, it can be usefully used for environmentally friendly and safe organic matter treatment by suppressing the growth of odors and harmful bacteria generated during the organic matter decomposition process.
- Aspergillus sydowii of the antibacterial decomposer of the present invention Shows antibacterial activity.
- Figure 2 shows the food waste decomposition activity of the antibacterial decomposer of the present invention.
- A distilled water treatment group
- B antibacterial decomposer treatment group.
- the present invention provides an antibacterial decomposer containing a mixed strain having antibacterial activity and organic matter decomposition activity as an active ingredient.
- the mixed strain is preferably Bacillus subtilis , Bacillus licheniformis , Bacillus sonorensis , Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ), Lactobacillus casei , Lactobacillus pentosus , Lactobacillus plantarum , Lactococcus lactis and Streptomyces sp. It may consist of strains, but is not limited thereto.
- the antibacterial decomposer of the present invention may preferably be a 'culture' of the mixed strain, more preferably Bacillus subtilis, Bacillus licheniformis, Bacillus sonorensis, Bacillus amyloliquefaciens, Lactobacillus casei, Lactobacillus pentosus, Lactobacillus plantarum, and Lactococcus lactis strains may be mixed with a culture solution of a Streptomyces genus strain, most preferably Bacillus subtilis, Bacillus Leecheniformis, Bacillus sonorensis, Bacillus amyloliquefaciens, Lactobacillus casei, Lactobacillus pentosus, Lactobacillus plantarum and Lactococcus lactis strains in the culture medium of strains of the genus Streptomyces and It may be mixed by adding a culture medium, but is not limited thereto.
- the antimicrobial activity is Aspergillus sydowii and It may be antibacterial activity against Aspergillus flavus , but is not limited thereto.
- the organic matter may preferably be food waste, human waste, livestock manure, or sewage sludge, more preferably food waste, but is not limited thereto.
- the 'culture medium' may include a culture medium of the mixed strain of the present invention, a concentrate of the culture medium, or a dried product of the culture medium, but is not limited thereto.
- the culture solution may be used in the form of the culture itself containing the mixed strain of the present invention, or may be subjected to centrifugation or filtration to remove the culture medium in the culture solution and recover only the concentrated cells, such Steps can be performed as needed by those skilled in the art.
- Concentrated cells can be frozen or lyophilized according to conventional methods to preserve their activity.
- the culture can be obtained by culturing in large quantities by a conventional microbial culture method, and a medium containing a carbon source, a nitrogen source, vitamins and minerals can be used as the culture medium, and the carbon source includes starch, sucrose, Citrate, maltose, glucose, mannitol, glycerol or xylose may be used, but is not limited thereto.
- the method of culturing the strain of the present invention may be cultured according to a method commonly used in the art, and is not limited to a specific method.
- the strain or its culture obtained in the step of culturing the strain of the present invention is used as an additive
- the strain or the culture of the strain may be added as it is or used together with other additives, and may be appropriately used according to a conventional method.
- the mixing amount of the components can be suitably determined depending on the intended use thereof.
- the antimicrobial decomposer of the present invention can be formulated in various forms.
- the formulation can be prepared, for example, by adding a solvent and/or a carrier.
- the method for preparing the antimicrobial decomposer may use any method known in the art, and is not particularly limited to a specific method.
- the present invention also provides an antibacterial composition containing the antimicrobial decomposer as an active ingredient.
- the antimicrobial decomposer is as described above, Aspergillus sydowii and Aspergillus flavus Mixed strains having antibacterial activity against active ingredient Since it contains, it can be usefully used as an antibacterial, preferably antifungal composition.
- the present invention also provides a composition for decomposing organic matter containing the antimicrobial decomposer as an active ingredient.
- the antibacterial decomposer is as described above, and since it contains as an active ingredient a mixed strain having antibacterial activity and decomposition activity for organic matter, it can be usefully used as a composition for decomposing organic matter.
- the organic matter may preferably be food waste, human waste, livestock manure, or sewage sludge, more preferably food waste, but is not limited thereto.
- the present invention also provides a method for decomposing organic matter comprising the step of treating the organic matter with the antimicrobial decomposer.
- the antimicrobial decomposer is as described above.
- the organic matter may preferably be food waste, human waste, livestock manure, or sewage sludge, more preferably food waste, but is not limited thereto.
- Bacillus subtilis Bacillus subtilis , Bacillus licheniformis , Bacillus sonorensis , Bacillus amyloliquefaciens
- 4 types of lactic acid bacteria Lactobacillus casei , Lactobacillus pentosus , Lactobacillus plantarum , Lactococcus lactis
- Streptomyces sp It was purchased and used by the Korea Research Institute of Engineering (KCTC) (Table 1).
- Bacillus sutilis KCTC3135 Bacillus subtilis
- Bacillus licheniformis KCTC1659 Bacillus licheniformis
- Bacillus sonorensis KCTC13918 Bacillus sonorensis
- Bacillus amyloliquefaciens KCTC3002 Bacillus amyloliquefaciens
- Lactobacillus 4 types Lactobacillus casei KCTC3109 ( Lactobacillus casei ) Lactobacillus Pentosus KCTC3120 ( Lactobacillus pentosus ) Lactobacillus plantarum KCTC13093 ( Lactobacillus plantarum ) Lactococcus lactis KCTC3769 ( Lactococcus lactis ) etc Streptomyces genus KCTC12434BP ( Streptomyces genus KCTC12434BP ( Streptomyces genus KCTC12434BP ( Streptomyces genus KCTC
- bacillus Four types were inoculated into 20 ml of NB (nutrient broth) medium and cultured at 180 rpm, 35 ° C for 48 hours to adjust the OD value to 0.5.
- Four types of lactic acid bacteria were inoculated into 20 ml of MRS broth, respectively. and incubated at 37 ° C for 48 hours, and prepared by adjusting the OD value to 0.5.
- the eight strains were inoculated at 0.2% (v / v) in 5 L of JBT optimal medium (Table 2), and cultured at 33 ° C. for 24 hours at an aeration rate of 0.4 vvm and an agitation speed of 250 rpm.
- the Streptomyces genus strain is plated on TSA (tryptic soy agar) medium and cultured at 30 ° C for 3 to 4 weeks, and when the cultured strain forms spores, 150 ml of GSS liquid medium (glucose 20 g, soybean meal 25 g, soluble starch 10 g, beef extract 1 g, yeast extract 4 g, sodium chloride 2 g, dipotassium phosphate 0.25 g, calcium carbonate 2 g, distilled water 1 l) and incubated for 10 days at 30 ° C and 170 rpm. . Thereafter, the supernatant and the precipitate were separated by centrifugation at 45,000 rpm for 30 minutes.
- GSS liquid medium glucose 20 g, soybean meal 25 g, soluble starch 10 g, beef extract 1 g, yeast extract 4 g, sodium chloride 2 g, dipotassium phosphate 0.25 g, calcium carbonate 2 g, distilled water 1 l
- the supernatant was prepared by filtering with a 0.45 ⁇ m filter (sample 1), and 100 ml of acetone or ethanol was added to the precipitate, stirred at 170 rpm for 10 hours, and then centrifuged at 45,000 rpm for 30 minutes.
- the liquid was prepared by filtering through a 0.45 ⁇ m filter (Sample 2).
- Trimethoprim is one of the antibacterial substances used for the treatment of inflammation and is used as an effective and safe medicine listed on the WHO list of essential medicines. Accordingly, after mixing the sample 1 and sample 2 in the same amount (sample 3), the content of trimethoprim was measured through HPLC analysis under the conditions shown in Table 3 below.
- trimethoprim contained in the 3E-1 cell of the genus Streptomyces and its culture medium was 7243.77 mg/L.
- Aspergillus sidowii Aspergillus sydowii
- Aspergillus flavus Aspergillus flavus
- the antibacterial activity was analyzed through a disk diffusion method. 150 ⁇ l of the culture medium of each strain was spread on TSA medium and dried for 10 to 20 minutes, and then a sterilized 8 mm paper disk was placed on the TSA medium. Thereafter, 50 ⁇ l of the antibacterial decomposer of the present invention was dropwise applied to the paper disk and incubated at 37° C. for 24 hours, and then the ring of inhibition around the paper disk was observed. A group treated with distilled water, 99.9% (v/v) ethanol or 30% (v/v) ethanol was used as a control group.
- the antibacterial decomposer of the present invention has excellent antibacterial activity against Aspergillus sydowii and Aspergillus flavus (FIG. 1).
- food waste samples were prepared by grinding ?? in a blender. 200 ml of the antibacterial decomposer of the present invention was added to 40 g of the food waste sample, reacted at 50 °C and 100 rpm for 3 hours, dried at 90 °C, and weighed. 200 ml of distilled water was added instead of the antimicrobial decomposer and used as a control.
- the weight of the dry food waste in the control group was 19.5 g, whereas the weight of the dry food waste in the experimental group treated with the antibacterial decomposer was 1.7 g, confirming that the weight of the food waste was reduced by about 91.2% compared to the control group ( Fig. 2).
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Agronomy & Crop Science (AREA)
- Medicinal Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to an antimicrobial degrading agent containing, as an active ingredient, a mixed strain having antimicrobial activity and organic matter degradation activity, and the use thereof. The antimicrobial degrading agent has excellent antimicrobial activity and organic matter degradation activity and thus inhibits foul order and proliferation of harmful bacteria which occur during a degradation process of an organic matter, and may be utilized for treating an organic matter in environment-friendly and safe manner.
Description
본 발명은 항균 활성 및 유기물 분해 활성이 있는 혼합 균주를 유효성분으로 함유하는 항균분해제 및 이의 용도에 관한 것이다.The present invention relates to an antibacterial decomposer containing as an active ingredient a mixed strain having antibacterial activity and organic matter decomposition activity and its use.
국내 음식물 쓰레기는 수분 함량과 유기물 함량이 80% 이상으로 염도가 매우 높고 쉽게 부패하여 처리가 쉽지 않다. 2005년 이전까지는 음식물 쓰레기가 발열량이 낮고 수분 함량이 높아 소각 처리에 부적합하다고 생각되어 주로 매립하여 처리하였다. 그러나, 악취 및 침출수 등에 의한 2차 오염과 쓰레기 매립지의 사용 단축 등의 문제로 2005년부터 음식물 쓰레기 매립이 전면 금지되었으며, 또한 런던협약으로 인하여 2013년부터 폐기물의 해양 투기가 전면 금지됨에 따라 음식물 쓰레기 처리를 위한 새로운 방법에 대한 연구가 필요한 실정이다. Domestic food waste has a very high salinity with more than 80% of water and organic matter, and it is not easy to dispose of because it rots easily. Prior to 2005, food waste was considered unsuitable for incineration due to its low calorific value and high moisture content, and was mainly disposed of in landfills. However, food waste landfilling has been completely banned since 2005 due to problems such as secondary pollution by odor and leachate and reduced use of landfills. There is a need for research on new methods for treatment.
최근, 미생물을 이용한 음식물 쓰레기의 소멸화 처리 방식이 관심을 받고 있다. 미생물이 투입된 음식물을 호기적 조건 및 적합한 공정의 짧은 시간 내에 발효시킴으로써 음식물 폐기물의 양을 크게 감소시키고 기존 처리 방법의 문제로 지적된 2차 환경오염도 최소화하여 현재 음식물쓰레기 처리를 위한 가장 합리적인 대안이라 할 수 있다. 이러한 미생물을 이용한 소멸화 처리 방법은 생물학적 환경이 적절하게 조절된 장치 내에서 미생물의 대사 활동에 의해 음식물 쓰레기의 유기물을 물과 탄산가스로 분해하여 대기 중으로 휘발시키는 처리공정을 갖는다.Recently, a food waste extinction treatment method using microorganisms has attracted attention. By fermenting microorganisms-injected food within a short time under aerobic conditions and suitable processes, it greatly reduces the amount of food waste and minimizes secondary environmental pollution, which has been pointed out as a problem with existing treatment methods, and is currently the most reasonable alternative for treating food waste. can The extinction treatment method using such microorganisms has a treatment step of decomposing organic matters of food waste into water and carbon dioxide by the metabolic activity of microorganisms in a device in which the biological environment is appropriately controlled, and volatilizing them into the air.
그러나, 미생물을 이용하여 음식물 찌꺼기를 처리하는 과정 중에는 유기물 분해시 발생되는 심한 악취로 인해 사용이 기피되고 있고, 효능이 만족스럽지 못하여 실사용에서는 거의 사용되지 않고 있는 실정이다. 또한, 미생물에 의한 유기물 분해가 상온에서 이루어지면 병원균과 같은 유해균의 증식이 활발히 이루어져 문제가 되고 있다. 따라서, 항균 활성 및 유기물 분해능이 우수한 미생물 제제에 대한 연구가 필요하다. However, during the process of treating food waste using microorganisms, the use is avoided due to the strong odor generated during the decomposition of organic matter, and the efficiency is not satisfactory, so it is rarely used in practical use. In addition, when organic matter is decomposed by microorganisms at room temperature, harmful bacteria such as pathogens actively proliferate, which is a problem. Therefore, there is a need for research on microbial preparations with excellent antibacterial activity and organic matter degradation ability.
한편, 한국등록특허 제0363798호에는 '유기물 분해능과 탈취기능을 가지는 신규한 균주 및 이를 이용한 음식물 쓰레기 처리방법'에 대해 개시하고 있으며, 한국등록특허 제1431250호에는 '병원성 미생물의 항균활성을 가진 신규한 유산균과 유기물분해 활성을 갖는 신규 유용 미생물을 유효성분으로 함유하는 가금류용 발효사료 첨가제 조성물'에 대해 개시하고 있다. 하지만, 본 발명의 '항균 활성 및 유기물 분해 활성이 있는 혼합 균주를 유효성분으로 함유하는 항균분해제 및 이의 용도'에 대해서는 아직까지 개시된 바가 없다.On the other hand, Korean Patent No. 0363798 discloses 'a novel strain having organic decomposition and deodorizing function and a food waste treatment method using the same', and Korean Patent No. 1431250 discloses 'a new strain having antibacterial activity of pathogenic microorganisms. Disclosed is a fermented feed additive composition for poultry, containing a lactic acid bacterium and a novel useful microorganism having organic matter decomposition activity as active ingredients. However, the 'antibacterial decomposer containing a mixed strain having antibacterial activity and organic matter decomposition activity as an active ingredient and its use' of the present invention has not been disclosed yet.
본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명자들은 바실러스균 4종, 유산균 4종 및 스트렙토마이세스 속 균주로 이루어진 혼합 균주를 유효성분으로 함유하는 항균분해제를 제조하였고, 상기 항균분해제가 항균 활성 및 유기물 분해 활성이 우수한 것을 확인함으로써, 본 발명을 완성하였다.The present invention has been derived from the above needs, and the present inventors have prepared an antimicrobial decomposition agent containing as an active ingredient a mixed strain consisting of 4 types of Bacillus bacteria, 4 types of lactic acid bacteria and a strain of the genus Streptomyces, and the antimicrobial decomposition The present invention was completed by confirming that the antimicrobial activity and organic matter decomposition activity were excellent.
상기 과제를 해결하기 위해, 본 발명은 항균 활성 및 유기물 분해 활성이 있는 혼합 균주를 유효성분으로 함유하는 항균분해제를 제공한다.In order to solve the above problems, the present invention provides an antibacterial decomposer containing a mixed strain having antibacterial activity and organic matter decomposition activity as an active ingredient.
또한, 본 발명은 상기 항균분해제를 유효성분으로 함유하는 항균용 조성물을 제공한다. In addition, the present invention provides an antibacterial composition containing the antimicrobial decomposer as an active ingredient.
또한, 본 발명은 상기 항균분해제를 유효성분으로 함유하는 유기물 분해용 조성물을 제공한다. In addition, the present invention provides a composition for decomposing organic matter containing the antimicrobial decomposer as an active ingredient.
또한, 본 발명은 상기 항균분해제를 유기물에 처리하는 단계를 포함하는 유기물을 분해하는 방법을 제공한다. In addition, the present invention provides a method of decomposing organic matter comprising the step of treating the organic matter with the antimicrobial decomposer.
본 발명의 항균분해제는 항균 활성 및 유기물 분해 활성이 우수하므로, 유기물 분해 과정 중에 발생하는 악취 및 유해균 증식을 억제하여 환경친화적이고 안전한 유기물 처리에 유용하게 활용될 수 있을 것이다. Since the antibacterial decomposer of the present invention has excellent antibacterial activity and organic matter decomposition activity, it can be usefully used for environmentally friendly and safe organic matter treatment by suppressing the growth of odors and harmful bacteria generated during the organic matter decomposition process.
도 1은 본 발명의 항균분해제의 아스퍼질러스 시도위(Aspergillus sydowii) 및 아스퍼질러스 플라부스(Aspergillus flavus)에 대한 항균 활성을 나타낸 것이다. A: 증류수 처리군, B: 99.9%(v/v) 에탄올 처리군, C: 30%(v/v) 에탄올 처리군, D: 항균분해제 처리군.1 is Aspergillus sydowii of the antibacterial decomposer of the present invention. And Aspergillus flavus ( Aspergillus flavus ) Shows antibacterial activity. A: Distilled water treatment group, B: 99.9% (v / v) ethanol treatment group, C: 30% (v / v) ethanol treatment group, D: antibacterial decomposer treatment group.
도 2는 본 발명의 항균분해제의 음식물 쓰레기 분해 활성을 나타낸 것이다. A: 증류수 처리군, B: 항균분해제 처리군.Figure 2 shows the food waste decomposition activity of the antibacterial decomposer of the present invention. A: distilled water treatment group, B: antibacterial decomposer treatment group.
본 발명의 목적을 달성하기 위하여, 본 발명은 항균 활성 및 유기물 분해 활성이 있는 혼합 균주를 유효성분으로 함유하는 항균분해제를 제공한다.In order to achieve the object of the present invention, the present invention provides an antibacterial decomposer containing a mixed strain having antibacterial activity and organic matter decomposition activity as an active ingredient.
본 발명의 항균분해제에서, 상기 혼합 균주는 바람직하게는 바실러스 서틸리스(Bacillus subtilis), 바실러스 리케니포미스(Bacillus licheniformis), 바실러스 소노렌시스(Bacillus sonorensis), 바실러스 아밀로리퀘파시언스(Bacillus amyloliquefaciens), 락토바실러스 카제이(Lactobacillus casei), 락토바실러스 펜토서스(Lactobacillus pentosus), 락토바실러스 플란타룸(Lactobacillus plantarum), 락토코커스 락티스(Lactococcus lactis) 및 스트렙토마이세스 속(Streptomyces sp.) 균주로 이루어진 것일 수 있으나, 이에 제한되지 않는다.In the antimicrobial decomposer of the present invention, the mixed strain is preferably Bacillus subtilis , Bacillus licheniformis , Bacillus sonorensis , Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ), Lactobacillus casei , Lactobacillus pentosus , Lactobacillus plantarum , Lactococcus lactis and Streptomyces sp. It may consist of strains, but is not limited thereto.
또한, 본 발명의 항균분해제는 바람직하게는 상기 혼합 균주의 '배양액'일 수 있고, 더욱 바람직하게는 바실러스 서틸리스, 바실러스 리케니포미스, 바실러스 소노렌시스, 바실러스 아밀로리퀘파시언스, 락토바실러스 카제이, 락토바실러스 펜토서스, 락토바실러스 플란타룸 및 락토코커스 락티스 균주의 배양액에 스트렙토마이세스 속 균주의 배양액을 첨가하여 혼합한 것일 수 있으며, 가장 바람직하게는 바실러스 서틸리스, 바실러스 리케니포미스, 바실러스 소노렌시스, 바실러스 아밀로리퀘파시언스, 락토바실러스 카제이, 락토바실러스 펜토서스, 락토바실러스 플란타룸 및 락토코커스 락티스 균주의 배양 배지에 스트렙토마이세스 속 균주의 균체 및 배양배지를 첨가하여 혼합한 것일 수 있으나, 이에 제한되지 않는다. In addition, the antibacterial decomposer of the present invention may preferably be a 'culture' of the mixed strain, more preferably Bacillus subtilis, Bacillus licheniformis, Bacillus sonorensis, Bacillus amyloliquefaciens, Lactobacillus casei, Lactobacillus pentosus, Lactobacillus plantarum, and Lactococcus lactis strains may be mixed with a culture solution of a Streptomyces genus strain, most preferably Bacillus subtilis, Bacillus Leecheniformis, Bacillus sonorensis, Bacillus amyloliquefaciens, Lactobacillus casei, Lactobacillus pentosus, Lactobacillus plantarum and Lactococcus lactis strains in the culture medium of strains of the genus Streptomyces and It may be mixed by adding a culture medium, but is not limited thereto.
본 발명의 일 구현 예에 다른 항균분해제에 있어서, 상기 항균 활성은 아스퍼질러스 시도위(Aspergillus sydowii) 및 아스퍼질러스 플라부스(Aspergillus flavus)에 대한 항균 활성일 수 있으나, 이에 제한되지 않는다. In another antibacterial decomposer in one embodiment of the present invention, the antimicrobial activity is Aspergillus sydowii and It may be antibacterial activity against Aspergillus flavus , but is not limited thereto.
또한, 상기 유기물은 바람직하게는 음식물 쓰레기, 인분, 축분 또는 하수 슬러지일 수 있으며, 더욱 바람직하게는 음식물 쓰레기일 수 있으나, 이에 제한되지 않는다. In addition, the organic matter may preferably be food waste, human waste, livestock manure, or sewage sludge, more preferably food waste, but is not limited thereto.
본 발명의 항균분해제에 있어서, 상기 '배양액'은 본 발명의 혼합 균주의 배양액, 상기 배양액의 농축액, 또는 상기 배양액의 건조물을 포함할 수 있으나, 이에 제한되지 않는다.In the antimicrobial decomposition agent of the present invention, the 'culture medium' may include a culture medium of the mixed strain of the present invention, a concentrate of the culture medium, or a dried product of the culture medium, but is not limited thereto.
또한, 상기 배양액은 본 발명의 혼합 균주를 포함하는 배양물 자체의 형태로도 사용할 수 있으며, 또는 배양액 중의 배양배지를 제거하고 농축된 균체만을 회수하기 위해 원심분리 또는 여과과정을 거칠 수 있으며, 이러한 단계는 당업자가 필요에 따라 수행할 수 있다. 농축된 균체는 통상적인 방법에 따라 냉동(frozen)하거나 또는 냉동건조(lyophilized)하여 그 활성을 잃지 않도록 보존할 수 있다.In addition, the culture solution may be used in the form of the culture itself containing the mixed strain of the present invention, or may be subjected to centrifugation or filtration to remove the culture medium in the culture solution and recover only the concentrated cells, such Steps can be performed as needed by those skilled in the art. Concentrated cells can be frozen or lyophilized according to conventional methods to preserve their activity.
상기 배양물은 통상적인 미생물의 배양방법에 의해 대량으로 배양하여 수득할 수 있으며, 배양배지로는 탄소원, 질소원, 비타민 및 미네랄을 포함하는 배지를 사용할 수 있으며, 상기 탄소원으로는 전분, 수크로즈, 시트레이트, 말토스, 글루코스, 만니톨, 글리세롤 또는 자일로스를 사용할 수 있으나, 이에 제한되지 않는다.The culture can be obtained by culturing in large quantities by a conventional microbial culture method, and a medium containing a carbon source, a nitrogen source, vitamins and minerals can be used as the culture medium, and the carbon source includes starch, sucrose, Citrate, maltose, glucose, mannitol, glycerol or xylose may be used, but is not limited thereto.
본 발명의 균주를 배양하는 방법은 당업계에 통상적으로 이용되는 방법에 따라 배양할 수 있으며, 특별한 방법에 한정되는 것은 아니다.The method of culturing the strain of the present invention may be cultured according to a method commonly used in the art, and is not limited to a specific method.
본 발명의 균주를 배양하는 단계에서 얻어지는 상기 균주 또는 이의 배양물을 첨가제로 사용할 경우, 상기 균주 또는 상기 균주의 배양물을 그대로 첨가하거나 다른 첨가제를 함께 사용할 수 있으며, 통상적인 방법에 따라 적절하게 사용될 수 있다. 성분의 혼합양은 그의 사용 목적에 따라 적합하게 결정될 수 있다.When the strain or its culture obtained in the step of culturing the strain of the present invention is used as an additive, the strain or the culture of the strain may be added as it is or used together with other additives, and may be appropriately used according to a conventional method. can The mixing amount of the components can be suitably determined depending on the intended use thereof.
또한, 본 발명의 항균분해제는 다양한 형태로 제제화할 수 있다. 상기 제제는 예를 들어 용매 및/또는 담체를 첨가함으로써 제조될 수 있다.In addition, the antimicrobial decomposer of the present invention can be formulated in various forms. The formulation can be prepared, for example, by adding a solvent and/or a carrier.
또한, 상기 항균분해제의 제조 방법은 당업계에 공지된 임의의 방법을 이용할 수 있으며, 특정 방법에 특별히 제한되는 것은 아니다. In addition, the method for preparing the antimicrobial decomposer may use any method known in the art, and is not particularly limited to a specific method.
본 발명은 또한, 상기 항균분해제를 유효성분으로 함유하는 항균용 조성물을 제공한다. The present invention also provides an antibacterial composition containing the antimicrobial decomposer as an active ingredient.
본 발명의 항균용 조성물에 있어서, 상기 항균분해제는 전술한 바와 같으며, 아스퍼질러스 시도위(Aspergillus sydowii) 및 아스퍼질러스 플라부스(Aspergillus flavus)에 대한 항균 활성이 있는 혼합 균주를 유효성분으로 함유하므로, 항균용, 바람직하게는 항진균용 조성물로 유용하게 사용될 수 있다. In the antimicrobial composition of the present invention, the antimicrobial decomposer is as described above, Aspergillus sydowii and Aspergillus flavus Mixed strains having antibacterial activity against active ingredient Since it contains, it can be usefully used as an antibacterial, preferably antifungal composition.
본 발명은 또한, 상기 항균분해제를 유효성분으로 함유하는 유기물 분해용 조성물을 제공한다. The present invention also provides a composition for decomposing organic matter containing the antimicrobial decomposer as an active ingredient.
본 발명의 유기물 분해용 조성물에 있어서, 상기 항균분해제는 전술한 바와 같으며, 항균 활성 및 유기물 분해 활성이 있는 혼합 균주를 유효성분으로 함유하므로, 유기물 분해용 조성물로 유용하게 사용될 수 있다.In the composition for decomposing organic matter of the present invention, the antibacterial decomposer is as described above, and since it contains as an active ingredient a mixed strain having antibacterial activity and decomposition activity for organic matter, it can be usefully used as a composition for decomposing organic matter.
또한, 상기 유기물은 바람직하게는 음식물 쓰레기, 인분, 축분 또는 하수 슬러지일 수 있으며, 더욱 바람직하게는 음식물 쓰레기일 수 있으나, 이에 제한되지 않는다.In addition, the organic matter may preferably be food waste, human waste, livestock manure, or sewage sludge, more preferably food waste, but is not limited thereto.
본 발명은 또한, 상기 항균분해제를 유기물에 처리하는 단계를 포함하는 유기물을 분해하는 방법을 제공한다. The present invention also provides a method for decomposing organic matter comprising the step of treating the organic matter with the antimicrobial decomposer.
본 발명의 유기물을 분해하는 방법에 있어서, 상기 항균분해제는 전술한 바와 같다.In the method of decomposing organic matter of the present invention, the antimicrobial decomposer is as described above.
또한, 상기 유기물은 바람직하게는 음식물 쓰레기, 인분, 축분 또는 하수 슬러지일 수 있으며, 더욱 바람직하게는 음식물 쓰레기일 수 있으나, 이에 제한되지 않는다.In addition, the organic matter may preferably be food waste, human waste, livestock manure, or sewage sludge, more preferably food waste, but is not limited thereto.
이하, 본 발명의 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, an embodiment of the present invention will be described in detail. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited to the following examples.
제조예 1. 항균분해제의 제조Preparation Example 1. Preparation of antibacterial decomposer
본 발명에서는 바실러스 4종(Bacillus subtilis, Bacillus licheniformis, Bacillus sonorensis, Bacillus amyloliquefaciens), 유산균 4종(Lactobacillus casei, Lactobacillus pentosus, Lactobacillus plantarum, Lactococcus lactis) 및 스트렙토마이세스 속(Streptomyces sp.) 균주를 한국생명공학연구원(KCTC)에서 분양받아 사용하였다(표 1). In the present invention, 4 types of Bacillus ( Bacillus subtilis , Bacillus licheniformis , Bacillus sonorensis , Bacillus amyloliquefaciens ), 4 types of lactic acid bacteria ( Lactobacillus casei , Lactobacillus pentosus , Lactobacillus plantarum , Lactococcus lactis ) and Streptomyces sp. It was purchased and used by the Korea Research Institute of Engineering (KCTC) (Table 1).
| 구분division | 균주명strain name |
|
바실러스 4종bacillus 4 types |
바실러스 서틸리스 KCTC3135 (Bacillus subtilis)Bacillus sutilis KCTC3135 ( Bacillus subtilis ) |
|
바실러스 리케니포미스 KCTC1659 (Bacillus licheniformis)Bacillus licheniformis KCTC1659 ( Bacillus licheniformis ) |
|
|
바실러스 소노렌시스 KCTC13918 (Bacillus sonorensis)Bacillus sonorensis KCTC13918 ( Bacillus sonorensis ) |
|
|
바실러스 아밀로리퀘파시언스 KCTC3002 (Bacillus amyloliquefaciens)Bacillus amyloliquefaciens KCTC3002 ( Bacillus amyloliquefaciens ) |
|
|
유산균 4종Lactobacillus 4 types |
락토바실러스 카제이 KCTC3109 (Lactobacillus casei)Lactobacillus casei KCTC3109 ( Lactobacillus casei ) |
|
락토바실러스 펜토서스 KCTC3120 (Lactobacillus pentosus)Lactobacillus Pentosus KCTC3120 ( Lactobacillus pentosus ) |
|
|
락토바실러스 플란타룸 KCTC13093 (Lactobacillus plantarum)Lactobacillus plantarum KCTC13093 ( Lactobacillus plantarum ) |
|
|
락토코커스 락티스 KCTC3769 (Lactococcus lactis)Lactococcus lactis KCTC3769 ( Lactococcus lactis ) |
|
| 기타etc |
스트렙토마이세스 속 KCTC12434BP (Streptomyces sp.)Streptomyces genus KCTC12434BP ( Streptomyces sp.) |
(1) 바실러스 및 유산균 균주의 배양(1) Cultivation of Bacillus and Lactobacillus strains
바실러스 4종은 20 ㎖의 NB(nutrient broth) 배지에 각각 접종하고 180 rpm, 35℃에서 48시간 배양하여 OD값을 0.5로 조정하여 준비하였으며, 유산균 4종은 20 ㎖의 MRS 액체배지에 각각 접종하고 37℃에서 48시간 배양하여 OD값을 0.5로 조정하여 준비하였다. 상기 8종의 균주를 5 ℓ의 JBT 최적 배지(표 2)에 0.2%(v/v)씩 각각 접종하고 33℃에서 통기량 0.4 vvm, 교반속도 250 rpm으로 24시간 동안 배양하여 준비하였다. Four types of bacillus were inoculated into 20 ml of NB (nutrient broth) medium and cultured at 180 rpm, 35 ° C for 48 hours to adjust the OD value to 0.5. Four types of lactic acid bacteria were inoculated into 20 ml of MRS broth, respectively. and incubated at 37 ° C for 48 hours, and prepared by adjusting the OD value to 0.5. The eight strains were inoculated at 0.2% (v / v) in 5 L of JBT optimal medium (Table 2), and cultured at 33 ° C. for 24 hours at an aeration rate of 0.4 vvm and an agitation speed of 250 rpm.
|
배지 성분 medium component |
g/L g/L | 조성비(%)composition ratio (%) |
| 펩톤(peptone)peptone | 3.03.0 | 10.810.8 |
| 분리대두단백(soy protein isolate)soy protein isolate | 1.51.5 | 5.45.4 |
| 옥수수침지액(corn steep liquor)Corn steep liquor | 0.10.1 | 0.40.4 |
| 당밀(molasses)molasses | 9.09.0 | 32.532.5 |
| 효모 추출물(yeast extract)yeast extract | 4.04.0 | 14.414.4 |
| 육즙 추출물(meat extract)meat extract | 4.54.5 | 16.216.2 |
| 덱스트로스(dextrose)dextrose | 5.05.0 | 18.118.1 |
| 염화칼륨(potassium chloride)potassium chloride | 0.20.2 | 0.70.7 |
| 황산마그네슘·7수화물(MgSO4·7H2O)Magnesium sulfate heptahydrate (MgSO 4 7H 2 O) | 0.10.1 | 0.40.4 |
| 염화나트륨(sodium chloride)sodium chloride | 0.30.3 | 1.11.1 |
| 계total | 27.727.7 | 100100 |
(2) 스트렙토마이세스 속 균주의 배양(2) Cultivation of strains of the genus Streptomyces
스트렙토마이세스 속 균주는 TSA(tryptic soy agar) 배지에 도말하여 30℃에서 3~4주 동안 배양한 후, 상기 배양된 균주가 포자를 형성하면 150 ㎖의 GSS 액체배지(glucose 20 g, soybean meal 25 g, soluble starch 10 g, beef extract 1 g, yeast extract 4 g, sodium chloride 2 g, dipotassium phosphate 0.25 g, calcium carbonate 2 g, 증류수 1 ℓ)에 접종하여 30℃, 170 rpm으로 10일간 배양하였다. 이후, 45,000 rpm으로 30분간 원심분리하여 상층액과 침전물을 분리하였다. 상기 상층액은 0.45 μm의 필터로 여과하여 준비하였고(시료 1), 상기 침전물에 아세톤 또는 에탄올을 100 ㎖ 첨가하고 170 rpm에서 10시간 동안 교반한 후, 45,000 rpm으로 30분간 원심분리하여 수득한 상층액을 0.45 μm의 필터로 여과하여 준비하였다(시료 2). The Streptomyces genus strain is plated on TSA (tryptic soy agar) medium and cultured at 30 ° C for 3 to 4 weeks, and when the cultured strain forms spores, 150 ml of GSS liquid medium (glucose 20 g, soybean meal 25 g, soluble starch 10 g, beef extract 1 g, yeast extract 4 g, sodium chloride 2 g, dipotassium phosphate 0.25 g, calcium carbonate 2 g, distilled water 1 ℓ) and incubated for 10 days at 30 ° C and 170 rpm. . Thereafter, the supernatant and the precipitate were separated by centrifugation at 45,000 rpm for 30 minutes. The supernatant was prepared by filtering with a 0.45 μm filter (sample 1), and 100 ml of acetone or ethanol was added to the precipitate, stirred at 170 rpm for 10 hours, and then centrifuged at 45,000 rpm for 30 minutes. The liquid was prepared by filtering through a 0.45 μm filter (Sample 2).
트리메소프림(trimethoprim)은 염증 치료에 사용되는 항균물질 중 하나로, WHO 필수 의약품 목록에 등재되어 있는 효과적이고 안전한 의약품으로 사용되고 있다. 이에, 상기 시료 1과 시료 2를 동일한 양으로 혼합한 후(시료 3), 하기 표 3과 같은 조건으로 HPLC 분석을 통해 트리메소프림의 함량을 측정하였다. Trimethoprim is one of the antibacterial substances used for the treatment of inflammation and is used as an effective and safe medicine listed on the WHO list of essential medicines. Accordingly, after mixing the sample 1 and sample 2 in the same amount (sample 3), the content of trimethoprim was measured through HPLC analysis under the conditions shown in Table 3 below.
| 구분division | 조건condition |
| Instrument Instrument | Agilent 1260 InfinityAgilent 1260 Infinity |
| DetectorDetector | UV detectorUV detector |
| WavelengthWavelength | 285 nm285 nm |
| ColumnColumn | Aegispak C18-L 250 x 4.6 mm, 5 μmAegispak C18-L 250 x 4.6 mm, 5 µm |
| TempTemp | 40 ℃40 ℃ |
| Mobile phaseMobile phase | 50 mM ammonium acetate(pH 4.5):ACN = 73:2750 mM ammonium acetate (pH 4.5):ACN = 73:27 |
| Flow rateFlow rate | 1 ㎖/min1 mL/min |
| InjectionInjection | 10 ㎕10 μl |
그 결과, 스트렙토마이세스 속 3E-1 균체 및 그 배양 배지에 함유된 트리메소프림의 함량은 7243.77 ㎎/ℓ인 것으로 확인하였다. As a result, it was confirmed that the content of trimethoprim contained in the 3E-1 cell of the genus Streptomyces and its culture medium was 7243.77 mg/L.
(3) 항균분해제의 제조(3) Preparation of antibacterial decomposer
상기 시료 3에 트리메소프림이 100 ppm의 농도로 포함되어 있도록 계산한 배양액을, 상기 제조예 (1)의 바실러스 및 유산균 균주의 배양액을 원심분리하여 수득한 상층액 1 ℓ에 첨가하고 혼합하여 항균분해제를 제조하였다.In the above sample 3 A culture solution calculated to contain trimesoprim at a concentration of 100 ppm was added to 1 liter of the supernatant obtained by centrifuging the culture solution of the Bacillus and lactic acid bacteria strains of Preparation Example (1) and mixed to prepare an antibacterial decomposer did
실시예 1. 항균분해제의 항균 활성 분석Example 1. Analysis of antibacterial activity of antimicrobial decomposer
아스퍼질러스 시도위(Aspergillus sydowii) 및 아스퍼질러스 플라부스(Aspergillus flavus)를 이용한 디스크 확산법을 통해 항균 활성을 분석하였다. 각 균주의 배양액 150 ㎕를 TSA 배지에 도말하고 10~20분 동안 건조시킨 후, 멸균된 8 ㎜ 페이퍼 디스크를 TSA 배지 위에 올려놓았다. 이후, 본 발명의 항균분해제 50 ㎕를 상기 페이퍼 디스크에 점적하고 37℃, 24시간 동안 배양한 다음 페이퍼 디스크 주변의 억제환을 관찰하였다. 증류수, 99.9%(v/v) 에탄올 또는 30%(v/v) 에탄올 처리군을 대조군으로 이용하였다. Aspergillus sidowii ( Aspergillus sydowii ) and Aspergillus flavus ( Aspergillus flavus ) The antibacterial activity was analyzed through a disk diffusion method. 150 μl of the culture medium of each strain was spread on TSA medium and dried for 10 to 20 minutes, and then a sterilized 8 mm paper disk was placed on the TSA medium. Thereafter, 50 μl of the antibacterial decomposer of the present invention was dropwise applied to the paper disk and incubated at 37° C. for 24 hours, and then the ring of inhibition around the paper disk was observed. A group treated with distilled water, 99.9% (v/v) ethanol or 30% (v/v) ethanol was used as a control group.
그 결과, 본 발명의 항균분해제는 아스퍼질러스 시도위(Aspergillus sydowii) 및 아스퍼질러스 플라부스(Aspergillus flavus)에 대한 항균 활성이 우수한 것을 확인하였다(도 1).As a result, it was confirmed that the antibacterial decomposer of the present invention has excellent antibacterial activity against Aspergillus sydowii and Aspergillus flavus (FIG. 1).
실시예 2. 항균분해제의 음식물 쓰레기 분해 효과 분석Example 2. Analysis of food waste decomposition effect of antibacterial decomposer
본 발명의 항균분해제의 음식물 쓰레기 분해 효과를 분석하기 위해 ??을 믹서기에 갈아서 음식물 쓰레기 샘플을 준비하였다. 상기 음식물 쓰레기 샘플 40 g에 본 발명의 항균분해제 200 ㎖을 첨가하고 50℃, 100 rpm으로 3시간 동안 반응시킨 후, 90℃에서 건조하고 무게를 측정하였다. 항균분해제 대신 증류수 200 ㎖을 첨가하여 대조군으로 이용하였다.In order to analyze the food waste decomposition effect of the antibacterial decomposer of the present invention, food waste samples were prepared by grinding ?? in a blender. 200 ml of the antibacterial decomposer of the present invention was added to 40 g of the food waste sample, reacted at 50 °C and 100 rpm for 3 hours, dried at 90 °C, and weighed. 200 ml of distilled water was added instead of the antimicrobial decomposer and used as a control.
그 결과, 대조군의 건조한 음식물 쓰레기의 무게는 19.5 g인 반면, 항균분해제를 처리한 실험군의 건조한 음식물 쓰레기의 무게는 1.7 g으로, 대조군 대비 음식물 쓰레기의 무게가 약 91.2% 감소하는 것을 확인하였다(도 2). As a result, the weight of the dry food waste in the control group was 19.5 g, whereas the weight of the dry food waste in the experimental group treated with the antibacterial decomposer was 1.7 g, confirming that the weight of the food waste was reduced by about 91.2% compared to the control group ( Fig. 2).
Claims (6)
- 항균 활성 및 유기물 분해 활성이 있는 혼합 균주를 유효성분으로 함유하는 항균분해제.An antibacterial decomposer containing a mixed strain having antibacterial activity and organic matter decomposition activity as an active ingredient.
- 제1항에 있어서, 상기 혼합 균주는 바실러스 서틸리스(Bacillus subtilis), 바실러스 리케니포미스(Bacillus licheniformis), 바실러스 소노렌시스(Bacillus sonorensis), 바실러스 아밀로리퀘파시언스(Bacillus amyloliquefaciens), 락토바실러스 카제이(Lactobacillus casei), 락토바실러스 펜토서스(Lactobacillus pentosus), 락토바실러스 플란타룸(Lactobacillus plantarum), 락토코커스 락티스(Lactococcus lactis) 및 스트렙토마이세스 속(Streptomyces sp.) 균주로 이루어진 것을 특징으로 하는 항균분해제.The method of claim 1, wherein the mixed strain is Bacillus subtilis, Bacillus licheniformis , Bacillus sonorensis , Bacillus amyloliquefaciens , lactose Characterized in that it consists of strains of Lactobacillus casei , Lactobacillus pentosus , Lactobacillus plantarum , Lactococcus lactis and Streptomyces sp. Antibacterial decomposer to.
- 제1항에 있어서, 상기 항균 활성은 아스퍼질러스 시도위(Aspergillus sydowii) 및 아스퍼질러스 플라부스(Aspergillus flavus)에 대한 항균 활성인 것을 특징으로 하는 항균분해제.The antimicrobial decomposer according to claim 1, wherein the antibacterial activity is antimicrobial activity against Aspergillus sydowii and Aspergillus flavus .
- 제1항 내지 제3항 중 어느 한 항의 항균분해제를 유효성분으로 함유하는 항균용 조성물.Claims 1 to 3 of any one of the antibacterial composition containing the antibacterial decomposition agent as an active ingredient.
- 제1항 내지 제3항 중 어느 한 항의 항균분해제를 유효성분으로 함유하는 유기물 분해용 조성물.A composition for decomposing organic matter containing the antibacterial decomposer according to any one of claims 1 to 3 as an active ingredient.
- 제1항 내지 제3항 중 어느 한 항의 항균분해제를 유기물에 처리하는 단계를 포함하는 유기물을 분해하는 방법.A method for decomposing organic matter comprising the step of treating the organic matter with the antimicrobial decomposer of any one of claims 1 to 3.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020210151947A KR102587464B1 (en) | 2021-11-08 | 2021-11-08 | Antimicrobial decomposer comprising microorganism mixture having antimicrobial activity and decomposable activity of organic waste as effective component and uses thereof |
| KR10-2021-0151947 | 2021-11-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2023080304A1 true WO2023080304A1 (en) | 2023-05-11 |
Family
ID=86241703
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/KR2021/016287 WO2023080304A1 (en) | 2021-11-08 | 2021-11-10 | Antimicrobial degrading agent containing, as active ingredient, mixed strain having antimicrobial activity and organic matter degradation activity, and use thereof |
Country Status (2)
| Country | Link |
|---|---|
| KR (1) | KR102587464B1 (en) |
| WO (1) | WO2023080304A1 (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101029346B1 (en) * | 2009-10-28 | 2011-04-13 | 주식회사 엠투원 | Combined microorganism fermented liquors having the effect of reducing odor gas and antibacterial effect, and methods of using the same |
| KR20160018572A (en) * | 2013-05-20 | 2016-02-17 | 바이오위시 테크놀로지스, 인크. | Microbial-based waste water treatment compositions and methods of use thereof |
| KR20190028407A (en) * | 2019-03-08 | 2019-03-18 | 주식회사 템셀코리아 | Composition for decomposing a food waste |
| KR102177670B1 (en) * | 2020-07-28 | 2020-11-11 | 김주석 | Composition for removing odor and, manufacturing method thereof |
| CN112574911A (en) * | 2020-12-11 | 2021-03-30 | 四川省丹丹郫县豆瓣集团股份有限公司 | Fermentation composition for inhibiting generation of aspergillus flavus and preparation method thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130118625A (en) * | 2012-04-20 | 2013-10-30 | 조선대학교산학협력단 | Lactobacillus plantarum with antifungal activity and an antifungal composition comprising the same |
| KR101379230B1 (en) * | 2012-07-20 | 2014-03-28 | 재단법인 순창군발효미생물관리센터 | Bacillus licheniformis strain SCK B11 having antimicrobial activity against pathogenic microorganism of soy sauce and degrading activity of biogenic amine and uses thereof |
| KR101467990B1 (en) * | 2013-08-22 | 2014-12-02 | 충남대학교산학협력단 | Composition for antibacterial compound containing Streptomyces sp. 3E-1 or its culture fluid as an active ingredient |
| KR101731034B1 (en) * | 2014-11-18 | 2017-04-27 | 주식회사 한울 | Bacillus amyloliquefaciens Ba3 strain having turf trash and thatch - decomposing activity and antifungal activity against plant pathogen, and microbial agent containing the same |
| KR102004089B1 (en) * | 2017-10-19 | 2019-07-25 | 재단법인 전라북도생물산업진흥원 | Mixed strain for decomposing food waste and Decomposition method for food waste using same |
-
2021
- 2021-11-08 KR KR1020210151947A patent/KR102587464B1/en active IP Right Grant
- 2021-11-10 WO PCT/KR2021/016287 patent/WO2023080304A1/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101029346B1 (en) * | 2009-10-28 | 2011-04-13 | 주식회사 엠투원 | Combined microorganism fermented liquors having the effect of reducing odor gas and antibacterial effect, and methods of using the same |
| KR20160018572A (en) * | 2013-05-20 | 2016-02-17 | 바이오위시 테크놀로지스, 인크. | Microbial-based waste water treatment compositions and methods of use thereof |
| KR20190028407A (en) * | 2019-03-08 | 2019-03-18 | 주식회사 템셀코리아 | Composition for decomposing a food waste |
| KR102177670B1 (en) * | 2020-07-28 | 2020-11-11 | 김주석 | Composition for removing odor and, manufacturing method thereof |
| CN112574911A (en) * | 2020-12-11 | 2021-03-30 | 四川省丹丹郫县豆瓣集团股份有限公司 | Fermentation composition for inhibiting generation of aspergillus flavus and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| KR102587464B1 (en) | 2023-10-10 |
| KR20230066693A (en) | 2023-05-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mohagheghi et al. | Isolation and characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a new genus of thermophilic, acidophilic, cellulolytic bacteria | |
| Hayakawa et al. | New methods for the highly selective isolation of Micromonospora and Microbispora from soil | |
| US6025187A (en) | Combinations of bacillus and lactobacillus species for producing protein from waste | |
| KR102177670B1 (en) | Composition for removing odor and, manufacturing method thereof | |
| KR100805036B1 (en) | Novel Bacillus cereus ENB-02 strain having foodwaste decompositing capability and microbial agent | |
| CN100579920C (en) | Domestic sewage process method | |
| KR100805044B1 (en) | Bacillus clausii strain for fats degradation and microbial agent for foodwaste treatment using it | |
| Thompson et al. | Survival of two ecologically distinct bacteria (Flavobacterium and Arthrobacter) in unplanted and rhizosphere soil: laboratory studies | |
| WO2023080304A1 (en) | Antimicrobial degrading agent containing, as active ingredient, mixed strain having antimicrobial activity and organic matter degradation activity, and use thereof | |
| Ezaki et al. | Biphenomycin A production by a mixed culture | |
| CN113621537B (en) | Novel bacterial strain BD-1 for degrading phenylacetic acid and application thereof | |
| CN107022504B (en) | Lactic acid bacteria with formaldehyde degradation effect and application thereof | |
| Kishimoto et al. | Growth inhibition of Acidiphilium species by organic acids contained in yeast extract | |
| KR102326124B1 (en) | Microbial agent of gel formulation for decomposing food waste and uses thereof | |
| Wang et al. | Isolation and identification of ammonia reducing Bacillus megaterium from chicken cecum | |
| CN110317764B (en) | Compound microbial agent and application thereof | |
| KR102238653B1 (en) | Novel Streptomyces sp. capable of degrading organochlorine pesticide in soil and use of the same | |
| JP4267384B2 (en) | New Bacillus sp., Garbage treatment agent, garbage treatment method and apparatus using the same | |
| KR100443267B1 (en) | Novel Bacillus licheniformis H-3 strain having capability of food waste decomposition | |
| KR20210157038A (en) | Optimal medium composition for increasing growth of mixed strain of Bacillus and lactic acid bacteria and uses thereof | |
| KR100782048B1 (en) | 1-2 A novel strain of Bacillus sp. LS 1-2 that growing in citrus waste and the production method of antibiotic probiotics using the same | |
| KR100553377B1 (en) | Probiotic for Inhibiting of Diarrhea and Bloody Excrement of Livestock | |
| Oliveira et al. | Are Azospirillum bacteriocins produced and active in soil? | |
| CN114634903B (en) | Directional activation method of beneficial flora in soil and application of yeast lysate in lactic acid bacteria growth promotion | |
| Karnetová et al. | Production of phenazines by Streptomyces cinnamonensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 21963404 Country of ref document: EP Kind code of ref document: A1 |