CN114634903B - Directional activation method of beneficial flora in soil and application of yeast lysate in lactic acid bacteria growth promotion - Google Patents
Directional activation method of beneficial flora in soil and application of yeast lysate in lactic acid bacteria growth promotion Download PDFInfo
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Abstract
The invention belongs to the technical field of farmland improvement and soil microorganism, and particularly relates to a directional activation method of beneficial bacteria in soil. The directional activation method of beneficial bacteria in soil comprises the following steps: s1, preparing lactic acid yeast composite bacteria liquid: adding a saccharomycete lysate into the lactobacillus fermentation liquid to obtain a lactobacillus composite bacterial liquid; s2, application of lactic acid yeast complex bacteria liquid: the lactic acid yeast composite bacteria liquid is applied into soil, the growth and propagation of lactic acid bacteria are promoted by the yeast lysate, and the growth and propagation of beneficial bacteria are further promoted by the growth and propagation of lactic acid bacteria. The invention establishes a directional activation method of beneficial bacteria in soil, which adds saccharomycete lysate to lactobacillus fermentation liquor to lead lactobacillus to be fixed and propagated in cultivated soil, and further activates beneficial bacteria such as spore bacteria, photosynthetic bacteria and actinomycetes in the cultivated soil in a directional manner, thereby playing a role in inhibiting plant germ growth in the cultivated soil.
Description
Technical Field
The invention belongs to the technical field of farmland improvement and soil microorganism, and particularly relates to a directional activation method of beneficial bacteria in soil.
Background
The long-term and large-scale use of chemical fertilizers and chemical pesticides leads to the rapid decrease of the abundance of beneficial bacteria in crop habitats such as cultivated land soil, and harmful microorganisms gradually occupy dominant ecological niches, further leads to the decrease of the quality of the cultivated land soil, and seriously affects the yield and quality of agricultural products. Activating beneficial bacteria of the cultivated land soil, and has positive significance for preventing and controlling cultivated land soil-borne diseases.
In the field of soil improvement, beneficial bacteria such as lactobacillus and saccharomycetes are common strains of microbial agents.
The microbial restoration agent for restoring the soil ecosystem disclosed in the Chinese patent document CN103627658A is prepared by mixing fermentation broths of various strains according to a proportion, and takes bacillus megaterium, bacillus licheniformis, clostridium butyricum and microzyme as main bacteria and takes lactobacillus and streptomyces microflavus as auxiliary bacteria. After entering the soil ecosystem, the microbial remediation agent can form dominant flora with indigenous beneficial microorganisms, and promote nitrogen and oxygen circulation of the soil ecosystem, so that the soil ecosystem is remedied, a new stable balanced ecosystem is formed, the growth environment of crops is improved, and the quality and yield of crops are improved.
A soil remediation agent disclosed in Chinese patent document CN106433680A comprises the following components: photosynthetic bacteria, lactic acid bacteria, bacillus subtilis, actinomycetes, bacteroides fragilis, candida utilis, bacillus natto, rhizobium and the like. The scheme adopts the mixture of a plurality of strains, and the photosynthetic bacteria have unique disease resistance and growth promotion capability; bacillus subtilis has direct or indirect inhibition effect on some pathogenic bacteria; actinomycetes fix nitrogen in the atmosphere; the bacteroides fragilis has strong anti-inflammatory property, can singly inhibit the generation of pro-inflammatory factors, and provides necessary nutrition for soil; the candida utilis assimilates nitrate carried by the fertilizer remained in the soil; the bacillus natto can decompose soil organic matters, supplement beneficial bacteria in the soil and inhibit growth and reproduction of harmful bacteria; rhizobia has nitrogen fixation effect and can increase nitrogen in soil.
Chinese patent document CN108728108A discloses a medicament for contaminated soil remediation, which consists of microbial agents and chemical agents, wherein the microbial agents include indigenous microbial cultures, nitrifying bacteria cultures, denitrifying bacteria cultures, polyphosphoric bacteria cultures, saccharomycetes cultures, lactic acid bacteria cultures. The indigenous microorganisms obtained by the culture medium intensified culture are helpful for rapidly repairing the polluted soil, the nitrifying bacteria, the denitrifying bacteria and the phosphorus accumulating bacteria have the functions of forming a stable nitrogen and phosphorus environment in the polluted soil, and the saccharomycetes and the lactic acid bacteria have the functions of keeping a stable organic matter environment in the polluted soil.
The technical proposal comprises saccharomycetes and lactobacillus, but the functions of the 2 strains in each proposal are different. The three technical schemes have the following defects: only the types and abundance of beneficial microorganisms in the soil ecosystem are focused on, and the colonization ability of exogenous bacterial species in the new soil environment is not considered.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a directional activation method for beneficial bacteria in soil, which promotes the stationary reproduction of lactic acid bacteria by adding yeast lysate into lactic acid bacteria fermentation liquor, and further promotes the directional activation of beneficial bacteria such as spore bacteria, photosynthetic bacteria, actinomycetes and the like in cultivated land soil, thereby inhibiting the growth of plant pathogenic bacteria.
In order to achieve the above object, the technical scheme of the present invention is as follows:
a directional activation method of beneficial bacteria in soil comprises the following steps:
s1, preparing lactic acid yeast composite bacteria liquid: adding a saccharomycete lysate into the lactobacillus fermentation liquid to obtain a lactobacillus composite bacterial liquid;
s2, application of lactic acid yeast complex bacteria liquid: the lactic acid yeast composite bacteria liquid is applied into soil, the growth and propagation of lactic acid bacteria are promoted by the yeast lysate, and the growth and propagation of beneficial bacteria are further promoted by the growth and propagation of lactic acid bacteria.
As an improvement, the preparation method of the saccharomycete lysate in the step S1 is as follows: inoculating yeast into YPDA liquid culture medium, culturing to obtain yeast liquid, ultrasonic-crushing yeast liquid at 40kHz for 10min, and intermittently crushing for 1min for 15-25 times.
As a further improvement, the preparation method of the saccharomycete lysate in the step S1 is as follows:
s11, adding saccharomycetes into YPDA culture solution for culture to obtain saccharomycetes solution;
s12, mixing the yeast liquid obtained in the step S11 with an improved YPDA culture liquid, and continuing fermenting until the yeast dies and cracks, wherein the improved YPDA culture liquid comprises the following components: 5.0-15.0g/L yeast extract, 15.0-25.0g/L peptone and 2.0-5.0g/L glucose.
As a further improvement, the saccharomycete liquid and the modified type YPDA culture solution are prepared according to the ratio of 1-10:1000 by volume.
As an improvement, in the step S1, the saccharomycete lysate is saccharomycete lysate, and the saccharomycete lysate and the lactic acid bacteria fermentation broth are mixed according to a ratio of 5-15:1000 by volume.
As an improvement, in the step S2, the application amount of the lactic acid yeast complex liquid is as follows: 2-8 kg of the soil is applied to each 100 kg of soil.
The invention also discloses application of the saccharomycete lysate in promoting the growth of the lactic acid bacteria, and experiments prove that the promoting effect of the saccharomycete lysate on the lactic acid bacteria is far better than the promoting effect of the living saccharomycete liquid on the lactic acid bacteria.
The invention establishes a directional activation method of beneficial bacteria in soil, which adds saccharomycete lysate to lactobacillus fermentation liquor to lead lactobacillus to be fixed and propagated in cultivated soil, and further activates beneficial bacteria such as spore bacteria, photosynthetic bacteria and actinomycetes in the cultivated soil in a directional manner, thereby playing a role in inhibiting plant germ growth in the cultivated soil.
Detailed Description
Example 1
The invention relates to a directional activation method of beneficial bacteria in soil, which comprises the following steps:
s1, preparation of lactic acid yeast composite bacterial liquid
Preparation of lactic acid bacteria fermentation liquor: selecting a lactococcus lactis strain screened from soil of a tomato planted in a certain continuous 3 years of a waterside area of a south-city in Huzhou as a test lactobacillus strain, inoculating the lactobacillus strain into a 1L conical flask filled with 500mL of MRS culture solution, and culturing at 37 ℃ with shaking for 24 hours; inoculating 500mL lactobacillus solution into 100L fermentation tank containing 50LMRS liquid culture medium by flame inoculation method, culturing at 37deg.C and 140rpm to OD 600 =2.16. The MRS culture solution comprises the following components: 10.0g/L peptone, 8.0g/L beef powder, 4.0g/L yeast powder, 20.0g/L glucose, 2.0g/L dipotassium hydrogen phosphate, 2.0g/L diammonium hydrogen citrate, 5.0g/L sodium acetate, 0.2g/L magnesium sulfate, 0.04g/L manganese sulfate, 1.0g/L Tween 80 and pH value of 5.7.
Preparing a saccharomycete liquid: selecting a strain of saccharomycete screened in soil of a tomato planted in a certain continuous 3 years of a waterside area in a city of Huzhou, and identifying the strain as the same strain as saccharomycete CCTCCWY2008374 preserved in a China center for type culture collection. The yeast was inoculated into a 100mL Erlenmeyer flask containing 30mL of YPDA medium and cultured at 28℃with shaking for 24 hours. The YPDA culture solution comprises the following components: yeast extract 10.0g/L, peptone 20.0g/L, glucose 20.0g/L, adenine sulfate 0.03g/L.
Preparation of yeast lysate: 2.5mL of saccharomycete liquid is mixed according to the volume ratio of 5:1000 was added to a 1L Erlenmeyer flask containing 500mL of the modified YPDA culture solution, and the resulting culture solution was a yeast lysate, and cultured at 28℃with shaking for 72 hours. The improved YPDA culture solution comprises the following components: yeast extract 10.0g/L, peptone 20.0g/L, glucose 3.0g/L.
Preparing lactic acid yeast composite bacterial liquid: 500mL of yeast lysate was added to 50L of lactic acid fermentation broth, i.e., 1:100 volume ratio, and fully stirring and uniformly mixing to obtain the lactic acid yeast composite bacterial liquid. The lactic acid yeast composite bacteria liquid is equally divided into 10 parts, and each part is 5L for standby.
S2, application of lactic acid yeast composite bacterial liquid
2000 kg of soil for planting tomatoes in a certain continuous 3 years of city in a Xun district of Huzhou is taken, fully and uniformly mixed, and the soil is divided into 20 parts with 100 kg each.
And 5L of lactic acid yeast composite bacteria liquid is applied into 100 kg of soil, fully and uniformly mixed, kept stand and incubated for 15 days, a certain amount of sterile water is sprayed every day, and the soil moisture is kept about 12%.
S3, soil flora structure analysis: after 15 days, the soil before and after the application of the lactic acid yeast complex liquid is subjected to flora structure analysis by adopting a high throughput sequencing technology, and the effects of the lactic acid yeast complex liquid on spore flora, photosynthetic flora, actinomycetes and 8 plant pathogenic bacteria (Fusarium oxysporum Schltdl. Root rot pathogenic bacteria; colletotrichum chlorophyti, anthracnose pathogenic bacteria; phytophthora capsici Leonian, pepper phytophthora pathogenic bacteria; leptosphaeria sp.F92, leaf blight pathogenic bacteria; phyllosticta capsici Speg, pepper leaf spot pathogenic bacteria; cercospora capsici Heald & F.A.wolf, pepper brown spot pathogenic bacteria; septoria lycopersici Speg. Tomato white spot pathogenic bacteria; fusarium graminearum Schwabe, root rot pathogenic bacteria) are examined by comparing and analyzing the soil flora structures before and after the application of the lactic acid yeast complex liquid.
The abundance conditions of lactococcus lactis, spore flora, photosynthetic flora and actinomycetes are shown in table 1 before and after the application of the lactic acid yeast compound bacterial liquid, and the result shows that the lactic acid yeast compound bacterial liquid can obviously activate the growth and propagation of soil spore flora, photosynthetic flora and actinomycetes, and simultaneously obviously reduce the abundance of 8 plant pathogenic bacteria in soil, thereby achieving the purpose of directional activation of beneficial flora in soil of a directional activated cultivated land.
TABLE 1 results of effect of lactic acid Yeast Complex liquid application on soil microbial abundance
Comparative example 1
Comparative example "Yeast lysate (Low sugar autophagy) was mixed with lactic acid bacteria broth in step S1 of example 1 at a ratio of 1:100 by volume ratio of the yeast fermentation broth to the lactobacillus fermentation broth according to a ratio of 1:100 by volume ratio "," mixing yeast lysate (ultrasonic disruption method) with lactobacillus fermentation broth according to 1:100 by volume ratio "," blank lysates were mixed with lactobacillus fermentation broth according to 1:100 by volume.
The preparation method of the yeast fermentation broth comprises the following steps: selecting a strain of saccharomycete screened in soil of a tomato planted in a certain continuous 3 years of a waterside area of a city, a south of Huzhou as a test saccharomycete strain, and identifying the strain as the same strain as saccharomycete CCTCC WY2008374 preserved in China center for type culture collection. The yeast is inoculated in a 100mL conical flask filled with 30mL of special culture medium YPDA for the yeast, and is cultured for 24 hours at the temperature of 28 ℃ with shaking, and then 2.5mL of yeast fermentation liquid is added according to the volume ratio of 5:1000 is added into a 1L conical flask filled with 500mL YPDA culture solution, and is cultured for 72 hours at 28 ℃ with shaking, thus obtaining the final saccharomycete fermentation broth. The YPDA liquid medium comprises the following components: yeast extract 10.0g/L, peptone 20.0g/L, glucose 20.0g/L, adenine sulfate 0.03g/L.
The preparation method of the saccharomycete lysate (ultrasonic disruption method) comprises the following steps: the preparation method of the yeast fermentation broth is the same as that described above, 500mL of the final yeast fermentation broth is subjected to ultrasonic crushing, 40kHz ultrasonic crushing for 10min and intermittent 1min, and the yeast lysate is obtained after crushing for 20 times (ultrasonic crushing method).
The control lysates were prepared as follows: culturing a 100mL conical flask which is not inoculated with yeast and is filled with 30mL of yeast special culture medium YPDA at 28 ℃ with shaking for 24 hours; then 2.5mL of culture solution is added according to the volume ratio of 5:1000 was added to a 1L Erlenmeyer flask containing 500mL of the modified YPDA medium, and the medium was cultured at 28℃with shaking for 72 hours, and the resulting medium was a blank lysate.
The yeast fermentation broth, the yeast lysate (ultrasonic disruption method) and the blank control lysate obtained in the comparative example 1 are respectively applied to the same three 100 kg of soil according to the same application proportion, fully and uniformly mixed, and are kept stand for 15 days for incubation, a certain amount of sterile water is sprayed every day, and the soil moisture is kept about 12%.
After 15 days, the flora structures before and after the application of the composite bacterial liquid to the three soils were analyzed by high throughput sequencing technology, and the activation effect of the yeast lysate (low sugar autophagy method), the yeast fermentation liquid (yeast live bacteria), the yeast lysate (ultrasonic disruption method) and the blank control lysate on the beneficial flora such as spore flora, photosynthetic flora and actinomycetes was examined by comparing with the soil flora structures before and after the application of the composite bacterial liquid to the three soils in application example 1. The abundance of lactococcus lactis, spore flora, photosynthetic flora, and actinomycetes in the soil before and after application is shown in table 2.
TABLE 2 results of effect of application of 4 lactic acid Complex bacterial liquids on soil spore flora, photosynthetic flora, actinomycete flora abundance
Table 2 the results show that: the abundance of lactococcus lactis in the 4 test groups of yeast lysate (low sugar autophagy), yeast fermentation broth (yeast live), yeast lysate (ultrasonication) and control lysate was significantly increased, because direct administration of the 4 lactic acid complex bacteria solutions containing a large amount of lactococcus lactis resulted in the soil, but the number of lactococcus lactis in the cultivated land soil of the other 3 test groups was significantly lower than that in the yeast lysate (low sugar autophagy) test group, wherein the minimum control lysate test group was 18.58% of the yeast lysate (low sugar autophagy) test group, the yeast fermentation broth (yeast live) and the yeast lysate (ultrasonication) test group were slightly higher than that in the control lysate test group, 30.53% and 31.86% of the yeast lysate (low sugar autophagy) test group, respectively, indicating that the yeast lysate (low sugar autophagy) can efficiently activate the determination of lactococcus lactis, whereas the active substance in the yeast lysate (ultrasonication) has a certain activity equivalent to that in the active medium of the active substance in the yeast lysate under high energy had a small amount of activated lactic acid bacteria.
Table 2 the results show that: the abundance of beneficial bacteria such as soil spore bacteria, photosynthetic bacteria and actinomycetes in the 4 test groups is increased, and the three beneficial bacteria abundance increases have positive correlation with the abundance of lactococcus lactis, which shows that the saccharomycete lysate further activates the beneficial bacteria such as spore bacteria, photosynthetic bacteria and actinomycetes in the cultivated land soil by promoting the growth of a large amount of lactobacillus.
Example 2
Example 2 differs from example 1 only in that: the lactobacillus and the saccharomycete are replaced, and the lactobacillus is lactobacillus plantarum, wherein the lactobacillus is lactobacillus CCTCCAB206127 preserved by the China center for type culture collection. The saccharomycete is selected from saccharomycete CCTCCDY 20081311 preserved in China center for type culture collection.
The method for applying the lactic acid yeast complex liquid was the same as in example 1, and after 15 days, the soil before and after applying the lactic acid yeast complex liquid was subjected to flora structure analysis by using a high throughput sequencing technology, and the results are shown in table 3.
TABLE 3 results of effect of lactic acid Yeast Complex liquid application on soil microbial abundance
The result shows that the lactic acid yeast composite bacterial liquid can obviously activate the growth and propagation of soil spore bacterial groups, photosynthetic bacterial groups and actinomycetes bacterial groups, and simultaneously obviously reduce the abundance of 8 plant pathogenic bacteria in soil, thereby achieving the purpose of directionally activating beneficial bacterial groups in soil of a directionally activated cultivated land.
Comparative example 2
Comparative example 2 was carried out in the same manner as in comparative example 1, and "the yeast lysate (low-sugar autophagy method) and the lactic acid bacteria fermentation broth were mixed in the same manner as in comparative example 2 at a ratio of 1:100 by volume ratio of the yeast fermentation broth to the lactobacillus fermentation broth according to a ratio of 1:100 by volume ratio "," mixing yeast lysate (ultrasonic disruption method) with lactobacillus fermentation broth according to 1:100 by volume ratio "," blank lysates were mixed with lactobacillus fermentation broth according to 1:100 by volume.
After 15 days using the same application method, the flora structures before and after the application of the composite bacterial liquid to the three soils were analyzed by high throughput sequencing technology, and the activation effects of the yeast lysate (low sugar autophagy method), the yeast fermentation liquid (yeast live bacteria), the yeast lysate (ultrasonic disruption method) and the blank control lysate on the beneficial flora such as spore flora, photosynthetic flora and actinomycetes were examined by comparing with the soil flora structures to which the lactic acid yeast composite bacterial liquid was applied in application example 2. The abundance of lactobacillus plantarum, bacillus, photosynthetic and actinomycetes before and after application is shown in table 4.
TABLE 4 Effect of application of 4 lactic acid Complex bacterial liquids on soil spore flora, photosynthetic flora, actinomycete flora abundance results
Table 4 the results show that: the abundance of lactobacillus plantarum in the 4 test groups of yeast lysate (low sugar autophagy), yeast fermentation broth (yeast live), yeast lysate (ultrasonication) and control lysate is significantly increased, because direct administration of the 4 lactic acid complex bacteria liquids containing a large amount of lactobacillus plantarum resulted in the soil, but the number of lactobacillus plantarum in the cultivated land soil of the other 3 test groups is significantly lower than that of the yeast lysate (low sugar autophagy) test group, wherein the minimum control lysate test group is 20.35% of the yeast lysate (low sugar autophagy) test group, the yeast fermentation broth (yeast live) and the yeast lysate (ultrasonication) test group are slightly higher than that of the control lysate test group, which are 28.76% and 31.42% of the yeast lysate (low sugar autophagy) test group, respectively, indicating that the yeast lysate (low sugar autophagy) can effectively activate the colonization of lactobacillus plantarum, and that the activity of the active substance in the yeast lysate (ultrasonication method) is partially inactive to the active lactobacillus plantarum under high energy effect is comparable to that of the active substance in the live bacteria.
Table 4 the results show that: the abundance of beneficial bacteria such as soil spore bacteria, photosynthetic bacteria and actinomycetes in the 4 test groups is increased, and the abundance of three beneficial bacteria has positive correlation with the abundance of lactobacillus plantarum, which shows that the saccharomycete lysate further activates the beneficial bacteria such as spore bacteria, photosynthetic bacteria and actinomycetes in the cultivated land soil by promoting the growth of a large amount of lactobacillus.
Example 3
This example examined the effect of yeast lysates and live yeasts on the cultivation of lactic acid bacteria, actinomycetes, spores and photosynthetic bacteria. The lactobacillus strain used for the experiment is purchased from the Siankang generation biotechnology Co., ltd and is lactobacillus casei (powder, 10 hundred million/g); the experimental yeast is purchased from Shandong Jining Jin Yi microorganism engineering Co., ltd and is Cryptococcus terrestris (powder, 2 hundred million/g); actinomycetes, bacillus and photosynthetic bacterial strains for the experiments were purchased from Cangzhou Hua Yu Biotechnology Co., ltd, as Streptomyces griseus (powder, 0.5 hundred million/g), bacillus licheniformis (powder, 100 hundred million/g) and cyanobacteria (powder, 2 hundred million/g), respectively. Purifying, separating and culturing saccharomycetes from saccharomycetes powder purchased from Shandong Jining Jin Yi microbial engineering Co., ltd to obtain saccharomycetes, and preparing saccharomycetes liquid by adopting the YPDA culture liquid culture method of the embodiment 1; yeast lysate was prepared by the low-sugar autophagy method of example 1, and 2L of yeast liquid (live yeast) and 2L of yeast lysate (low-sugar autophagy method) were obtained, respectively, for use.
8 parts of the soil from example 1 were taken, each 100 kg, and numbered sequentially.
1 kg (powder, 10 hundred million/g) of lactic acid bacteria are added into the No. 1 soil, and in theory, 1000 ten thousand lactic acid bacteria are contained in 1 g of soil. 500mL of yeast lysate was added and thoroughly mixed.
1 kg (powder, 10 hundred million/g) of lactic acid bacteria are added into No. 2 soil, and in theory, 1000 ten thousand lactic acid bacteria are contained in 1 g of soil. 500mL of yeast liquid is added and fully and uniformly mixed.
1 kg of actinomycetes (powder, 0.5 hundred million/g) is added to the No. 3 soil, and theoretically, 1 g of the soil contains 50 ten thousand actinomycetes. 500mL of yeast lysate was added and thoroughly mixed.
1 kg of actinomycetes (powder, 0.5 hundred million/g) is added to the No. 4 soil, and theoretically, 1 g of the soil contains 50 ten thousand actinomycetes. 500mL of yeast liquid is added and fully and uniformly mixed.
1 kg (100 hundred million/g) of bacillus is added to the soil of part 5, and 1 hundred million bacillus is theoretically contained in 1 g of soil. 500mL of yeast lysate was added and thoroughly mixed.
1 kg (100 hundred million/g) of bacillus is added to the No. 6 soil, and 1 hundred million bacillus is theoretically contained in 1 g of soil. 500mL of yeast liquid is added and fully and uniformly mixed.
1 kg (powder, 2 hundred million/g) of photosynthetic bacteria are added into the No. 7 soil, and theoretically, 1 g of soil contains 200 ten thousand photosynthetic bacteria. 500mL of yeast lysate was added and thoroughly mixed.
1 kg (powder, 2 hundred million/g) of photosynthetic bacteria are added into No. 8 soil, and theoretically, 1 g of soil contains 200 ten thousand photosynthetic bacteria. 500mL of yeast liquid is added and fully and uniformly mixed.
The 8 parts of soil are subjected to standing incubation for 15 days, a certain amount of sterile water is sprayed every day, and the soil moisture is kept about 12%.
After 15 days, the number and abundance of target bacteria in the soil before and after 8 application of the saccharomycete lysate or saccharomycete liquid were analyzed using a high throughput sequencing technique, wherein the results are shown in table 5, including lactobacillus casei for soil analysis No. 1 and No. 2, streptomyces griseus for soil analysis No. 3 and No. 4, bacillus licheniformis for soil analysis No. 5 and No. 6, and cyanobacteria for soil analysis No. 7 and No. 8.
TABLE 5 influence of Yeast solution and Yeast lysate on the growth of lactic acid bacteria, actinomycetes, spore bacteria, photosynthetic bacteria, respectively
As can be seen from table 5, both the yeast lysate and the yeast liquid can effectively promote the growth and reproduction of lactic acid bacteria, and the promoting effect of the yeast lysate remarkably optimizes the yeast liquid; the yeast lysate and the yeast liquid have no promotion effect on the growth and reproduction of 3 bacteria of Streptomyces griseus (actinomycetes), bacillus licheniformis (bacillus) and cyanobacteria (photosynthetic bacteria); the quantity of 3 bacteria of Streptomyces griseus (actinomycetes), bacillus licheniformis (Bacillus) and cyanobacteria (photosynthetic bacteria) is reduced, because beneficial bacteria cannot be well colonized after being applied into soil, and decay occurs. The results show that the saccharomycete lysate can obviously promote the growth and reproduction of lactic acid bacteria, and has no obvious effect of promoting the growth and reproduction of 3 bacteria of streptomyces griseus (actinomycetes), bacillus licheniformis (bacillus) and cyanobacteria (photosynthetic bacteria).
Claims (7)
1. A method for directionally activating beneficial bacteria in soil, which is characterized by comprising the following steps:
s1, preparing lactic acid yeast composite bacteria liquid: adding a saccharomycete lysate into the lactobacillus fermentation liquid to obtain a lactobacillus composite bacterial liquid;
s2, application of lactic acid yeast complex bacteria liquid: the lactic acid yeast composite bacterial liquid is applied into soil, the growth and reproduction of lactic acid bacteria are promoted by the yeast lysate, the growth and reproduction of lactic acid bacteria further promote the growth and reproduction of beneficial bacterial groups, wherein the beneficial bacterial groups are spore bacterial groups, photosynthetic bacterial groups or actinomycetes.
2. The method for directional activation of beneficial bacteria in soil according to claim 1, wherein the preparation method of the saccharomycete lysate in the step S1 is as follows: inoculating saccharomycetes into YPDA liquid culture medium for culturing to obtain saccharomycetes liquid, and ultrasonically crushing the saccharomycetes liquid.
3. The method for directionally activating beneficial bacteria in soil according to claim 2, wherein the mode of ultrasonic disruption of the saccharomycete liquid is as follows: crushing at 40kHz for 10min, intermittently for 1min, and crushing for 15-25 times.
4. The method for directional activation of beneficial bacteria in soil according to claim 1, wherein the preparation method of the saccharomycete lysate in the step S1 is as follows:
s11, adding saccharomycetes into YPDA culture solution for culture to obtain saccharomycetes solution;
s12, mixing the yeast liquid obtained in the step S11 with an improved YPDA culture liquid, and continuing fermenting until the yeast dies and cracks, wherein the improved YPDA culture liquid comprises the following components: 5.0-15.0g/L yeast extract, 15.0-25.0g/L peptone and 2.0-5.0g/L glucose.
5. The method for directional activation of beneficial bacteria in soil according to claim 4, wherein the yeast liquid and the modified YPDA culture liquid are mixed according to a ratio of 1-10:1000 by volume.
6. The method for directional activation of beneficial bacteria in soil according to claim 4, wherein in the step S1, the yeast lysate is a yeast lysate, and the yeast lysate and the lactic acid bacteria broth are mixed according to a ratio of 5-15:1000 by volume.
7. The method for directional activation of beneficial bacteria in soil according to claim 1, wherein in the step S2, the application amount of the lactic acid yeast complex is as follows: 2-8 kg of the soil is applied to each 100 kg of soil.
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