WO2023066029A1 - 光纤束插芯的制造方法及多通道光纤记录系统 - Google Patents

光纤束插芯的制造方法及多通道光纤记录系统 Download PDF

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Publication number
WO2023066029A1
WO2023066029A1 PCT/CN2022/123828 CN2022123828W WO2023066029A1 WO 2023066029 A1 WO2023066029 A1 WO 2023066029A1 CN 2022123828 W CN2022123828 W CN 2022123828W WO 2023066029 A1 WO2023066029 A1 WO 2023066029A1
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WIPO (PCT)
Prior art keywords
optical fiber
optical
positioning mold
fibers
fiber
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PCT/CN2022/123828
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English (en)
French (fr)
Inventor
毕国强
张轲铭
祝清源
刘北明
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中国科学技术大学
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Publication of WO2023066029A1 publication Critical patent/WO2023066029A1/zh

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
    • A61B5/316—Modalities, i.e. specific diagnostic methods
    • A61B5/388—Nerve conduction study, e.g. detecting action potential of peripheral nerves
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/255—Splicing of light guides, e.g. by fusion or bonding
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/36—Mechanical coupling means
    • G02B6/38—Mechanical coupling means having fibre to fibre mating means
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/42—Coupling light guides with opto-electronic elements

Definitions

  • the present disclosure relates to the field of neural signal recording, and in particular to a manufacturing method of an optical fiber bundle ferrule configured for neural signal recording and a multi-channel optical fiber recording system.
  • the brain is a high-level center that controls behavior and psychology, and contains a large number of neurons and other types of cells. Recording and interpreting the neural activity of the brain plays a vital role in understanding various behaviors and psychology, diagnosing and treating mental diseases, and developing artificial intelligence. Fiber-optic recording systems have been developed to observe neural activity in the brain.
  • a fiber-optic jumper is used to connect one end of the animal's brain and the other end of the recording device to collect neural activity signals in a single brain region.
  • the optical fiber recording system can only record the activity of a single brain region, and cannot simultaneously record signals from multiple brain regions.
  • the brain is usually active in multiple brain regions at the same time, and a single brain region record will inevitably lose a lot of information.
  • the optical fiber signal acquisition device of the optical fiber recording system is bulky, with a three-dimensional size usually ranging from tens of centimeters to tens of centimeters, and a weight usually ranging from several kilograms to tens of kilograms.
  • optical fiber recording system it is impossible to record for a long time, because the optical fiber jumper will be entangled with the movement of the animal; it is also impossible to record complex and violent behaviors, one is because the optical fiber jumper has certain rigidity and weight, which may hinder Animal activities; Second, the deformation of the optical fiber jumper caused by the violent activities of animals will also cause signal distortion.
  • a multi-fiber bundle jumper is used to record the activities of multiple brain regions.
  • the separated ends of the optical fiber bundle jumper are connected to different brain regions of the animal, and the integrated end is connected to the optical fiber acquisition device.
  • the multi-channel optical fiber recording system can record multiple brain regions, the signal is distorted due to its large size, heavy weight, optical fiber winding, and optical fiber deformation. Simultaneous connection loads in the brain severely hamper their application to small animals.
  • the present disclosure provides a method for manufacturing an optical fiber bundle ferrule and a multi-channel optical fiber recording system, so as to improve the ability of the optical fiber recording system to simultaneously record different brain regions. flexibility.
  • the present disclosure provides a method for manufacturing an optical fiber bundle ferrule, comprising: manufacturing an optical fiber positioning mold, forming a plurality of positioning holes on the optical fiber positioning mold; inserting the first ends of multiple optical fibers into the positioning holes of the optical fiber positioning mold Middle; the position of the exposed multiple optical fibers near the optical fiber positioning mold is fixed with the curing material and the optical fiber positioning mold, so as to keep the relative position of the multiple optical fibers inserted into one end of the optical fiber positioning mold unchanged; the second end of the multiple optical fibers part into the tubular part, so that the optical fiber positioned between the tubular part and the optical fiber positioning mold forms an umbrella part; utilize a curing material to fix the umbrella part and part of the tubular part to form a fixed part; and insert The optical fiber in the optical fiber positioning mold is drawn out from the optical fiber positioning mold.
  • the inserting the first ends of the plurality of optical fibers into the positioning holes of the optical fiber positioning mold respectively includes: manufacturing an optical fiber alignment plate, on which a plurality of The position and quantity of the positioning holes on the optical fiber positioning mold are the same, and the collimation holes configured for the optical fiber to pass through; the optical fiber alignment plate is placed in parallel above the optical fiber positioning mold, and positioned on the optical fiber In the direction of the orthographic projection of the mould, each of the collimation holes corresponds to the position of one of the positioning holes; the first ends of a plurality of the optical fibers are respectively passed through the corresponding collimation holes to pre-positioning the optical fibers; and inserting the first ends of each of the optical fibers into the positioning holes; wherein the alignment holes are through holes.
  • the exposed multiple optical fibers are fixed to the fiber positioning mold with a curing material near the fiber positioning mold, so as to keep the relative positions of the multiple optical fibers inserted into the fiber positioning mold unchanged. After that, it also includes removing the fiber collimating plate.
  • the positioning hole formed on the optical fiber positioning mold includes a blind hole or a diameter-reducing hole.
  • the tubular member after inserting the second ends of the plurality of optical fibers into the tubular member, it further includes: inserting one or more reference optical fibers into the plurality of optical fibers, so that the plurality of optical fibers The optical fibers are closely arranged around the reference optical fiber in concentric circles, squares, rectangles or other shapes, and one end of the reference optical fiber is close to the optical fiber positioning mold.
  • the cured material has light-shielding properties.
  • the method further includes: cutting off parts of the plurality of optical fibers exposing the tubular member.
  • the present disclosure also provides a multi-channel optical fiber recording system, including: an optical fiber bundle ferrule manufactured by the method for manufacturing an optical fiber bundle ferrule according to any one of the above-mentioned fiber optic bundle ferrules.
  • the measuring target is used to guide the excitation light to the measured target and collect the emitted light generated after the measured target is excited; and the optical fiber detection device is connected to the fiber bundle ferrule and is configured to generate the excited light, receive the emitted light, and Convert optical signals into electrical signals.
  • the optical fiber detection device includes: an optical fiber connector, and a tubular member of the optical fiber bundle ferrule is partially inserted into one end of the optical fiber connector, so as to realize the connection between the optical fiber connector and the optical fiber connector.
  • the optical transceiver includes: a housing including an interface optically coupled with the optical fiber connector; a light source disposed in the housing and configured to generate light beams; a first filter a light sheet, disposed in the housing, configured to filter the light beam from the light source to generate the excitation light; and an optical conversion assembly, disposed in the housing, configured to filter the beam from the light source to generate the excitation light; Excitation light from the first filter is directed to the fiber optic bundle ferrule and emission light from the fiber optic bundle ferrule is directed to the image sensor.
  • the optical conversion assembly includes: a dichroic mirror; an objective lens arranged between the dichroic mirror and the optical fiber connector, and the objective lens is arranged to receive The mirror reflects the excitation light from the first optical filter and the emission light from the fiber bundle ferrule, and further injects the excitation light into the fiber bundle ferrule; the eyepiece, the eyepiece is set and a second filter configured to filter the emitted light from the eyepiece and guide the filtered emitted light to the image sensor.
  • the optical fiber bundle ferrule includes: a plurality of optical fibers; the tubular member, the second ends of the plurality of optical fibers are held in the tubular member; and a fixing part, the The middle part of the optical fiber is fixed in the fixing part, and the first end part of the optical fiber protrudes from the fixing part so as to be inserted into the target to be measured.
  • the multi-channel optical fiber recording system further includes: a signal acquisition device configured to receive the electrical signal from the optical fiber detection device; and a reversing device coupled to the optical fiber detection device and Between the signal acquisition devices to avoid cable entanglement caused by the movement of the measured object.
  • the manufactured optical fiber bundle ferrule has high integration, small size, light weight, and the relative position of multiple optical fibers in the optical fiber bundle ferrule It can be set freely, so it has high flexibility to record different brain regions at the same time.
  • FIG. 1 is a flowchart of a method for manufacturing an optical fiber bundle ferrule according to an embodiment of the present disclosure
  • FIG. 2 is a schematic diagram of a multi-channel optical fiber recording system according to an embodiment of the present disclosure
  • FIG. 3 is a schematic diagram of an application scenario of a multi-channel optical fiber recording system according to an embodiment of the present disclosure
  • FIG. 4 is a schematic diagram of another application scenario of a multi-channel optical fiber recording system according to an embodiment of the present disclosure.
  • FIG. 5 is a schematic diagram of the operation process of a method for manufacturing an optical fiber bundle ferrule according to an embodiment of the present disclosure.
  • FIG. 6 is a comparison diagram of the angle deviation of the optical fiber caused by using a collimating plate to pre-position the optical fiber and not pre-positioning the optical fiber in the schematic diagram of the operation process shown in FIG. 5 .
  • 1 optical fiber connector
  • 2 tubular piece
  • 3 fixed part
  • 4 optical fiber
  • 5 light source
  • 6 condensing lens
  • 7 first filter
  • 8 dichroic mirror
  • 9 objective lens
  • 10 eyepiece
  • 11 second optical filter
  • 12 image sensor
  • 13 housing
  • 14 fiber bundle ferrule
  • 15 optical transceiver
  • 16 first reversing device
  • 17 signal acquisition device
  • 18 The second reversing device
  • 19 optical fiber positioning mold
  • 20 optical fiber alignment plate.
  • a method for manufacturing an optical fiber bundle ferrule including operation S100 to operation S600.
  • operation S100 includes: manufacturing an optical fiber positioning mold, and forming a plurality of positioning holes on the optical fiber positioning mold.
  • operation S200 includes: respectively inserting the first ends of the plurality of optical fibers into the positioning holes of the optical fiber positioning mold.
  • operation S300 includes: using a curing material to fix the positions of the multiple exposed optical fibers adjacent to the optical fiber positioning mold, so as to keep the relative positions of the multiple optical fibers inserted into one end of the optical fiber positioning mold unchanged. Change.
  • operation S400 includes: inserting the second ends of the plurality of optical fibers into the tubular member, so that the optical fibers located between the tubular member and the fiber positioning mold form an umbrella-shaped portion.
  • operation S500 includes: fixing the umbrella portion and part of the tubular member with a curing material to form a fixing portion.
  • operation S600 includes: extracting the optical fiber inserted into the optical fiber positioning mold from the optical fiber positioning mold.
  • operation S200 includes:
  • Step S210 Manufacture an optical fiber alignment plate, on which there are a plurality of alignment holes that are configured to pass through the optical fiber and are configured to pass through the optical fiber alignment plate in the same position and number as the positioning holes on the optical fiber positioning mold. .
  • Step S220 placing the fiber alignment plate in parallel above the fiber positioning mold, and making each alignment hole correspond to a position of a positioning hole in the direction of the orthographic projection of the fiber positioning mold.
  • Step S230 passing the first ends of the plurality of optical fibers through the corresponding collimating holes, so as to pre-position the optical fibers.
  • Step S240 inserting the first end of each optical fiber into the positioning hole respectively.
  • operation S300 further includes: fixing the exposed multiple optical fibers adjacent to the optical fiber positioning mold with a curing material and the optical fiber positioning mold, so as to maintain the relative position of the multiple optical fibers inserted into one end of the optical fiber positioning mold Once unchanged, remove the fiber alignment plate.
  • the positioning holes formed on the optical fiber positioning mold include but are not limited to blind holes or reduced diameter holes.
  • At least two of the blind holes have different depths.
  • the depths of the multiple blind holes formed on the optical fiber positioning mold are all different, so as to meet the requirement of the length of the optical fiber protruding from the fixing part.
  • a plurality of diameter-reducing holes are formed on the fiber positioning mold.
  • each reducing hole decreases along the depth direction from the opening position.
  • the diameter of the opening of the variable diameter hole is greater than or equal to the diameter of the optical fiber, so that the optical fiber can pass through, and the internal diameter of the variable diameter hole is smaller than the diameter of the optical fiber, so as to limit the suitable depth of the optical fiber inserted into the fiber positioning mold It is appropriate.
  • a multi-channel optical fiber recording system comprising:
  • At least one optical fiber bundle ferrule manufactured according to the manufacturing method of the above-mentioned optical fiber bundle ferrule, the optical fiber of the optical fiber bundle ferrule exposing the fixed part is inserted into the target to guide the excitation light to the target and collect the measured object.
  • the emission light generated after the target is excited; and the optical fiber detection device, connected with the fiber bundle ferrule, is configured to generate the excitation light, receive the emission light and convert the optical signal into an electrical signal.
  • the spatial positions between multiple optical fibers can be freely set according to the target to be measured, which improves the flexibility of simultaneously recording different targets to be measured.
  • FIG. 2 is a schematic diagram of a multi-channel fiber optic recording system according to one embodiment of the present disclosure.
  • the multi-channel optical fiber recording system of the embodiment of the present disclosure includes a fiber bundle ferrule 14 and an optical fiber detection device connected to the fiber bundle ferrule 14.
  • the emitted light generated after the target to be measured is excited; the optical fiber detection device is configured to generate the excited light, receive the emitted light and convert the optical signal into an electrical signal.
  • the fiber optic bundle ferrule 14 includes: a plurality of optical fibers 4, and the first ends (the lower end in FIG. 2 ) of the plurality of optical fibers 4 are inserted into the measured target (such as the brain of the measured animal) Tubular member 2, the second end portion (upper end in Fig. 2) of a plurality of said optical fibers 4 is held in said tubular member 2; Fixed part 3, the middle part of said optical fiber 4 is fixed on said fixed part Among them, the first end of the optical fiber 4 protrudes from the fixing part 3 to be inserted into the target to be measured.
  • the spatial position between the first ends of the optical fiber 4 can be set freely according to different objects to be measured.
  • the first end of the optical fiber 4 protrudes from the fixing part 3 so as to be inserted into the target to be measured, for example, into different targeted brain regions of the head of an animal.
  • the plurality of optical fibers 4 may be plastic optical fibers or optical fibers made of other materials.
  • the fixing part 3 fixes the middle parts of the multiple optical fibers 4 to ensure that the relative spatial positions of the first ends of the multiple optical fibers 4 remain unchanged.
  • the fixing part 3 is formed after the curing material cures the middle parts of the plurality of optical fibers 4 .
  • the curing material can be dental fluid resin, light curing glue, epoxy resin, silicone rubber, PDMS, agarose.
  • the curing material is a material with light-shielding properties.
  • a plurality of optical fibers 4 are closely arranged, and the tubular member 2 centrally fixes the second ends of the plurality of optical fibers 4 , and the second ends of the plurality of optical fibers 4 are held at the in the tubular member.
  • a tubular member 2 with an appropriate inner diameter is selected.
  • the tubular member 2 can be a tubular member or other fixed structures.
  • the tubular member 2 is a rigid capillary, and optionally, for example, the tubular member 2 is a stainless steel capillary.
  • the multiple optical fibers 4 include 6 plastic optical fibers with a diameter of 250 micrometers of different lengths, targeting 6 brain regions, the height of the fixing part 3 is 7 mm, and the fixing part 3 can be tapered or other shape, the tubular member 2 is 2 mm high.
  • the fiber bundle ferrule 14 further includes a reference fiber, which is not inserted into the target to be measured, but only reflects the excitation light, as a comparison of the optical signals in the multiple optical fibers 4 .
  • a plurality of optical fibers 4 are closely arranged in concentric circles, squares, rectangles or other shapes around the reference optical fiber.
  • the reference optical fiber is a plastic optical fiber with a diameter of 250 microns, and multiple optical fibers 4 are closely arranged in concentric circles around the reference optical fiber.
  • the end face of the reference optical fiber at one end of the first end of the optical fiber 4 is provided with a metallic paint coating, so as to enhance the optical signal in the reference optical fiber.
  • the optical fiber detection device includes: a fiber optic connector 1, the tubular member 2 of the fiber bundle ferrule is partially inserted into one end of the fiber optic connector, so as to realize multiple connections between the fiber optic connector 1 and the fiber bundle ferrule 14 optical coupling of the optical fiber 4; an optical transceiver 15 configured to generate the excitation light and receive the emitted light; and an image sensor 12 configured to receive the light according to the optical transceiver 15
  • the emitted light produces an electrical signal characterizing the image of the object under test.
  • the weight of the optical fiber detection device may be less than 2 grams.
  • the optical fiber detection device 15 measures 7 ⁇ 7 ⁇ 16 mm and weighs 0.8 grams.
  • the optical fiber connector 1 is a tubular connector, and the tubular member 2 of the optical fiber bundle ferrule 14 is partially inserted into one end of the optical fiber connector 1 to realize the optical fiber connector 1 Optically coupled with the multiple optical fibers 4 of the optical fiber bundle ferrule 14 .
  • the optical fiber connector 1 is a tubular connector with an outer dimension of 3 ⁇ 3 ⁇ 3 mm, and each of its opposite sides has a M0.6 miniature screw, which is configured to be fixed with the optical transceiver 15 .
  • the optical transceiver 15 includes: a housing 13, including an interface optically coupled with the optical fiber connector 1; a light source 5, disposed in the housing 13, configured to generate light beams;
  • the first optical filter 7 is arranged in the housing 13 and is configured to filter the light beam from the light source 5 to generate the excitation light; and an optical conversion component is arranged in the housing 13, configured to guide the excitation light from the first optical filter 7 to the fiber bundle ferrule 14, and guide the emission light from the fiber bundle ferrule 14 to the image sensor 12.
  • the housing 13 includes an interface optically coupled with the optical fiber connector 1 , and the inner surface of the housing 13 has an uneven structure to reduce stray light in the optical transceiver 15 .
  • the shading degree of the casing 13 is greater than 90%.
  • the housing 13 can be made of 3D printed black plastic or other light-shielding materials.
  • the light source 5 is a micro LED with a size of 1.7 ⁇ 1.3 ⁇ 0.4 mm, which is arranged on the lower right side of the inner wall of the casing 13 .
  • the micro-LED emits a blue light beam with a dominant wavelength of 470 nanometers.
  • the light source 5 can also be two micro-LEDs with different dominant wavelengths, which emit two-color light beams with two dominant wavelengths.
  • the light source 5 can also be a plurality of micro LEDs with different dominant wavelengths.
  • the first filter 7 is disposed on the side of the light source 5 away from the inner wall of the housing 13 , and its length and width are no greater than 5 mm.
  • the first optical filter 7 is a disc with a diameter of 2 mm, a thickness of 1 mm, a wavelength selection range of 460-480 nm, and an OD value of at least 6.
  • a condenser lens 6 may also be arranged between the light source 5 and the first filter 7 to collect the light beam generated by the light source 5 intensively.
  • the diameter of the condenser lens 6 is not greater than 5 mm.
  • the condenser lens 6 is a hemispherical lens with a diameter of 2 mm, and one end of the plane of the hemispherical lens is closely attached to the left side of the light source 5 .
  • the first optical filter 7 is vertically arranged on the left side of the condenser lens 6 , 0.1 mm away from the high point of the condenser lens 6 .
  • the optical conversion assembly includes: a dichroic mirror 8; an objective lens 9, which is arranged between the dichroic mirror 8 and the optical fiber connector 1, and the objective lens 9 is arranged to receive the light reflected by the dichroic mirror 8 from The excitation light of the first optical filter 7 and the emission light from the fiber bundle ferrule 14, and further the excitation light is incident on the fiber bundle ferrule 14; the eyepiece 10, the eyepiece 10 is set To accept the emitted light from the objective lens 9 transmitted by the dichroic mirror 8; and the second filter 11 is configured to filter the emitted light from the eyepiece 10, and will be filtered The emitted light is directed to the image sensor 12.
  • the optical conversion component is disposed on a side of the first filter 7 away from the light source 5 .
  • the upper end of the dichroic mirror 8 is inclined to the right and is adjacent to the first optical filter 7, the objective lens 9 is arranged below the dichroic mirror 8, the eyepiece 10 is arranged on the top of the dichroic mirror 8, and the second optical filter 11 Set above the eyepiece 10.
  • the upper end of the reflection and transmission surface of the dichroic mirror 8 is obliquely arranged on the other side of the first filter 7, forming a vertical angle of 45° with the first filter 7, and the width is not more than 5 mm.
  • the length of the dichroic mirror 8 is 5.2 millimeters, the width is 3 millimeters, the thickness is 1.1 millimeters, and the center wavelength is 500 nanometers.
  • the light sheet 7 is arranged at a vertical angle of 45°, and the center point of the dichroic mirror 8 is 1.8 millimeters away from the first filter 7 .
  • the objective lens 9 is located below the dichroic mirror 8 and has a diameter not larger than 5 mm.
  • the objective lens 9 is a biconvex lens with a diameter of 2 mm and a focal length of 2 mm.
  • the center point of the lower surface is 1.6 millimeters apart, and the center point of the objective lens 9 coincides with the center point of the reflection and transmission surface of the dichroic mirror 8 in the vertical direction.
  • the eyepiece 10 is above the dichroic mirror 8 and has a diameter not greater than 5 mm.
  • the eyepiece 10 is a biconvex lens with a diameter of 3 mm and a focal length of 6.3 mm, and is placed above the dichroic mirror 8 .
  • the second optical filter 11 is above the eyepiece 10 , and its length and width are no greater than 5 mm.
  • the second optical filter 11 is a disc with a diameter of 3.5 mm, a thickness of 1 mm, a wavelength selection range of 515-535 nm, and an OD value of at least 6, placed above the eyepiece 10, 0.1 mm away from the upper surface of the eyepiece 10.
  • the image sensor 12 is arranged outside the housing 13 at one end away from the fiber bundle ferrule 14, and is configured to generate and characterize the measured light according to the emitted light received by the optical transceiver 15. An electrical signal of an image of an object.
  • the image sensor 12 is a 600-line analog CMOS with a size of 6.5 ⁇ 6.5 ⁇ 2 mm, located above the second optical filter 11 , with a distance of 3.4 mm from the second optical filter 11 .
  • Fig. 3 is a schematic diagram of an application scenario of a multi-channel optical fiber recording system according to an embodiment of the present disclosure.
  • a fluorescent probe such as the calcium activity indicator GCaMP6s
  • a fluorescent probe should be injected into the target brain region in advance, and at the same time, the first part of the optical fiber 4 End insertions target brain regions.
  • the light beam emitted by the light source 5 is converged by the condenser lens 6 and then filtered and purified by the first filter 7 to form excitation light.
  • the excitation light is reflected by the dichroic mirror 8 into the objective lens 9, and is transmitted to the target brain area by the fiber optic bundle ferrule 14, so as to excite the calcium activity indicator GCaMP6s injected into the target brain area in advance.
  • the green fluorescent signal emitted by GCaMP6s after being excited is collected by the first end of the optical fiber 4 as emitted light.
  • the emitted light is received by the objective lens 9 through the fixed part 3 and the tubular member 2, received by the eyepiece 10 after being transmitted by the dichroic mirror 8, filtered and purified by the second filter 11, and finally imaged by the image sensor 12 at 25 frames per second. frequency collection.
  • the image sensor 12 converts the light signal of the emitted light into an electric signal.
  • the multi-channel optical fiber recording system can also include a signal acquisition device 17, the signal acquisition device 17 is coupled with the optical fiber detection device, and is configured to receive signals from the optical fiber detection device electric signal.
  • the capture device 17 is a computer installed with an analog video capture card.
  • the multi-channel optical fiber recording system may further include a reversing device coupled between the optical fiber detection device and the collection device 17 , configured to avoid cable entanglement caused by animal movement.
  • the reversing device can be a conductive slip ring or an active commutator.
  • the reversing device is a first reversing device 16, and the first reversing device 16 is 6.5 mm in diameter and 6.5 mm in length. 10mm 4-way conductive slip ring.
  • Fig. 4 is a schematic diagram of another application scenario of a multi-channel optical fiber recording system according to an embodiment of the present disclosure.
  • the reversing device may also include two first reversing devices 16 and a second reversing device 18, the second reversing device 18 is an 8-way conductive slip ring with a diameter of 6.5 mm and a length of 10 mm.
  • FIG. 1 is a flowchart of a method for manufacturing an optical fiber bundle ferrule according to an embodiment of the present disclosure.
  • an optical fiber bundle ferrule 14 comprising:
  • Operation S100 making an optical fiber positioning mold, and forming a plurality of positioning holes on the optical fiber positioning mold.
  • Operation S200 Insert the first ends of the plurality of optical fibers 4 into the positioning holes of the optical fiber positioning mold respectively.
  • Operation S300 Fix the exposed parts of the multiple optical fibers 4 near the fiber positioning mold with a curing material and the fiber positioning mold, so as to keep the relative positions of the multiple optical fibers 4 inserted into the fiber positioning mold unchanged.
  • Operation S400 Insert the second ends of the plurality of optical fibers 4 into the tubular member 2, so that the optical fibers 4 between the tubular member 2 and the optical fiber positioning mold form an umbrella-shaped portion.
  • Operation S500 Fix the umbrella-shaped part and part of the tubular member with a curing material to form a fixed part 3 .
  • Operation S600 extract the optical fiber 4 inserted into the optical fiber positioning mold from the optical fiber positioning mold.
  • FIG. 5 is a schematic diagram of an operation process of a method for manufacturing an optical fiber bundle ferrule according to an embodiment of the present disclosure.
  • FIG. 6 is a comparison diagram of the angle deviation of the optical fiber caused by using a collimating plate to pre-position the optical fiber and not pre-positioning the optical fiber in the schematic diagram of the operation process shown in FIG. 5 .
  • the manufacturing method of the fiber bundle ferrule 14 may specifically include steps S1-S14.
  • an engraving machine or a numerical control machine tool is used to drill positioning holes of appropriate depth and diameter at the corresponding positions of the animal skull model to make an optical fiber positioning mold.
  • the animal skull model can make a 1:1 skull model with a real skull structure for a small hard block of the same size as the animal head or 3D printing technology.
  • an optical fiber positioning mold is made for punching six positioning holes with a suitable depth and a diameter of 250 microns at corresponding positions on a transparent acrylic block with a size of 20 ⁇ 20 ⁇ 10 mm.
  • step S2 the optical fiber alignment plate 20 is manufactured according to the position and quantity of the positioning holes on the optical fiber positioning mold 19, and the aperture diameter of the optical fiber alignment plate is consistent with that of the positioning holes.
  • step S3 the first end portions of the plurality of optical fibers 4 are polished to be smooth without scratches.
  • 1500 mesh, 3000 mesh, 7000 mesh, 10000 mesh and 12000 mesh sandpaper were used to polish one end of seven plastic optical fibers with a length of 30 mm and a diameter of 250 microns to make them smooth without scratches.
  • step S4 the first ends of the plurality of optical fibers 4 are inserted into corresponding collimating holes provided on the fiber collimating plate 20, and the first ends are passed through the collimating holes.
  • step S5 the first ends of the plurality of optical fibers 4 are inserted into the positioning holes until greater resistance is encountered, and the remaining parts remain outside the optical fiber positioning mold.
  • 6 out of 7 optical fibers are inserted into the positioning holes of the optical fiber positioning mold.
  • step S6 the exposed parts of the plurality of optical fibers 4 adjacent to the optical fiber positioning mold are coated with a curing material.
  • black photocurable dental resin is used to smear the area where the surface of the optical fiber positioning mold and the 6 optical fibers are in contact, the thickness of the smeared resin is less than 2 mm, and it is cured by irradiating light with a wavelength of 450 nanometers.
  • the fiber collimation plate 20 is removed.
  • a release agent can be used to coat the surface of the optical fiber positioning mold before the curing material is applied, so as to reduce the resistance to removal after curing.
  • step S7 according to the number and diameter of multiple optical fibers 4, a tubular member 2 with an appropriate inner diameter is selected, and the second end of the optical fiber 4 outside the optical fiber positioning mold is inserted into the tubular member 2 by leaving the plurality of optical fibers 4 outside the optical fiber positioning mold, so that The optical fiber 4 located between the tubular member 2 and the optical fiber positioning mold forms an umbrella-shaped portion.
  • a stainless steel capillary with a length of 2 mm, an outer diameter of 1.8 mm, and an inner diameter of 0.8 mm is selected, and the second end of the optical fiber left outside the fiber positioning mold is inserted into the stainless steel capillary for about 3 mm. .
  • the reference optical fiber is inserted into the tubular member 2 so that the optical fiber 4 is arranged in concentric circles, squares, rectangles or other shapes surrounding the reference optical fiber, and one end of the reference optical fiber is close to the optical fiber positioning mold .
  • the seventh optical fiber is used as a reference optical fiber, inserted into the stainless steel capillary so that it is in the center of the remaining six optical fibers, and the seven optical fibers form a concentric circle, and the seventh optical fiber is inserted into the stainless steel capillary and positioned close to the optical fiber mold surface.
  • step S9 reflective paint is used to paint the end face of the reference optical fiber close to the optical fiber positioning mold.
  • metallic paint is used to coat the end face of the seventh optical fiber close to the fiber positioning mold.
  • step S10 the tubular member 2 after the optical fiber 4 is inserted is pushed towards the direction of the optical fiber positioning mold, as close as possible to the mold until encountering greater resistance, so that the optical fiber 4 located between the tubular member 2 and the optical fiber positioning mold form an umbrella;
  • step S11 the umbrella-shaped portion and part of the tubular member 2 are fixed with a curing material to form a fixed portion 3 .
  • black light-curable dental resin is used to coat the umbrella-shaped part and part of the tubular part 2 covering the fiber positioning mold, and is cured by irradiating light with a wavelength of 450 nanometers to form a tapered fixing part 3 .
  • step S12 the cured fiber bundle ferrule 14 is removed from the fiber positioning mold, and the part of the optical fiber 4 exposed outside the tubular member 2 is cut off with a fiber cutter.
  • the surface of the optical fiber bundle ferrule 14 may be coated with light-shielding paint to further enhance the light-shielding performance.
  • step S13 the end surface of the tubular member 2 is ground and polished, so that the end surfaces of the plurality of optical fibers 4 in the tubular member 2 are smooth and free of scratches.
  • sandpapers of 1500 mesh, 3000 mesh, 7000 mesh, 10000 mesh and 12000 mesh are respectively used to polish and polish the exposed end surface of the tubular member 2 .
  • step S14 the fiber bundle ferrule 14 is connected to the fiber optic connector 1 .
  • the fiber bundle ferrule 14 can be fixedly connected with an adhesive.
  • the manufacturing method of the fiber bundle ferrule is based on the above steps S1 to S14.
  • the method of using the fiber collimation plate is compared with the method of not using the fiber collimation plate.
  • the significance test result P-value ⁇ 0.01.
  • the relative positions of multiple optical fibers in the fiber optic bundle ferrule can be set freely, and at the same time target different brain regions, which improves the flexibility of recording different brain regions;
  • the bundles are closely arranged and highly integrated, so it is small in size and light in weight, and can be carried by the target to be measured;
  • a reversing device is used to avoid signal distortion caused by cable deformation, and to avoid cable entanglement caused by the movement of the target to be measured. Continuous recording for hours or even days is possible.
  • Words such as “first”, “second”, “third” and the like used in the description and claims to modify the corresponding elements do not in themselves mean that the elements have any ordinal numbers, nor The use of these ordinal numbers to represent the sequence of an element with respect to another element, or the order of manufacturing methods, is only used to clearly distinguish one element with a certain designation from another element with the same designation.

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Abstract

一种光纤束插芯（14）的制造方法，包括：制造光纤定位模具，在光纤定位模具上形成多个定位孔（S100）；将多根光纤（4）的第一端部分别插入光纤定位模具的定位孔中（S200）；对裸露在外的多根光纤（4）的临近光纤定位模具的部位利用固化材料与光纤定位模具进行固定，以保持多根光纤（4）插入光纤定位模具一端的相对位置不变（S300）；将多根光纤（4）的第二端部插进管状件（2）内，使得位于管状件（2）和光纤定位模具之间的光纤（4）形成伞状部（S400）；利用固化材料固定伞状部和部分管状件（2），以形成固定部（3）（S500）；以及将插入光纤定位模具中的光纤（4）从光纤定位模具抽出（S600）。还提供了一种基于光纤束插芯（14）的多通道光纤记录系统。

Description

光纤束插芯的制造方法及多通道光纤记录系统 技术领域
本公开涉及神经信号记录领域，尤其涉及一种被配置用于神经信号记录的光纤束插芯的制造方法及多通道光纤记录系统。
背景技术
大脑是控制行为和心理的高级中枢，包含大量的神经元和其它种类细胞。记录和解析大脑的神经活动对理解各种行为和心理、诊断和治疗精神疾病以及发展人工智能等方面具有至关重要的作用。目前已研发了光纤记录系统来观察大脑神经活动。
在已研发的一种光纤记录系统中，使用一根光纤跳线，一端连接动物脑部、一端连接记录设备来收集单个脑区的神经活动信号。该光纤记录系统只能记录单个脑区的活动，无法同时记录多个脑区的信号。而大脑通常是多个脑区同时活跃的，单脑区记录必将丢失很多信息。另外，该光纤记录系统的光纤信号采集装置体积大，三维尺寸通常在十几厘米至数十厘米，重量通常在数千克到十几千克。这样的体积和重量在实际应用中，设备只能放在架子上，通过光纤跳线连接到动物头部。在该光纤记录系统中，无法长时间记录，因为光纤跳线会随着动物的运动而缠绕；也无法记录复杂、剧烈的行为，一是因为光纤跳线具有一定的刚性和重量，可能会阻碍动物的活动；二是因为动物剧烈活动引起的光纤跳线的变形也会导致信号失真。
在已研发的一种多通道光纤记录系统中，采用一分多光纤束跳线记录多个脑区的活动。光纤束跳线分离的数个末端连接到动物的不同脑区，集成在一起的一端连接光纤采集装置。该多通道光纤记录系统虽然可以记录多个脑区，但体积大、重量沉、光纤缠绕、光纤变形致使信号失真，并且光纤束跳线的方案增加了动物头部的负担，多根光纤跳线同时连接在脑部的负荷严重影响了其在小型动物上的应用。
在已研发的一种高度集成的多通道光纤记录系统中，采用商业化的排孔连接器，将多根光纤做成植入动物脑内的光纤束插芯，并采用轻型的光纤跳线将多个脑区的活动信号传输到采集设备。该多通道光纤记录系统虽然提出了一种高密度光纤束插芯，但具有固定插孔位置的连接头限制了光纤末端分布的灵活性，光纤末端只能以固定倍数的间距分布，并且后端采集设备大、长时间记录下光纤跳线缠绕以及光纤变形导致的失真。
发明内容
基于现有技术中的上述、以及其他方面的技术至少一种技术问题，本公开提供了一种光纤束插芯的制造方法及多通道光纤记录系统，以提高光纤记录系统同时记录不同脑区的灵活性。
本公开提供一种光纤束插芯的制造方法，包括：制造光纤定位模具，在所述光纤定位模具上形成多个定位孔；将多根光纤的第一端部分别插入光纤定位模具的定位孔中；对裸露在外的多根光纤的临近光纤定位模具的部位利用固化材料与光纤定位模具进行固定，以保持多根光纤插入光纤定位模具一端的相对位置不变；将多根光纤的第二端部插进管状件内，使得位于所述管状件和光纤定位模具之间的光纤形成伞状部；利用固化材料固定所述伞状部和部分所述管状件，以形成固定部；以及将插入所述光纤定位模具中的光纤从所述光纤定位模具抽出。
在一种可能实施的方式中，所述将多根光纤的第一端部分别插入光纤定位模具的定位孔中，包括：制造光纤准直板，在所述光纤准直板上设置有多个与所述光纤定位模具上的定位孔的位置及数量一致的、被配置用于光纤穿过的准直孔；将所述光纤准直板平行放置于所述光纤定位模具的上方，并在所述光纤定位模具的正投影方向上、使每个所述准直孔与一个所述定位孔的位置相对应；将多根所述光纤的第一端部分别穿过相对应的所述准直孔，以对所述光纤进行预定位；以及将每个所述光纤的第一端部分别插入所述定位孔内；其中，所述准直孔为通孔。
在一种可能实施的方式中，其中，对裸露在外的多根光纤的临近光纤定位模具的部位利用固化材料与光纤定位模具进行固定，以保持多根光纤插入光纤定位模具一端的相对位置不变之后，还包括移除所述光纤准直板。
在一种可能实施的方式中，其中，形成于所述光纤定位模具上的所述定位孔包括盲孔或变径孔。
在一种可能实施的方式中，在所述将多根光纤的第二端部插进管状件内之后，还包括：在所述多根光纤中插入一根或多根参考光纤，使得多根光纤环绕参考光纤紧密排列成同心圆、方形、长方形或其它形状，并且所述参考光纤的一端靠近所述光纤定位模具。
在一种可能实施的方式中，所述固化材料具有遮光性能。
在一种可能实施的方式中，在所述将插入所述光纤定位模具中的光纤从所述光纤定位模具抽出之后，还包括：切除所述多根光纤的露出所述管状件的部分。
本公开还提供一种多通道光纤记录系统，包括：任一项所述的光纤束插芯的制造方法制造的光纤束插芯，所述光纤束插芯的露出所述固定部的光纤插入被测目标，以向所述被测目 标传导激发光并收集被测目标受激发后产生的发射光；以及光纤探测装置，与光纤束插芯连接，被配置用于产生激发光、接收发射光以及将光信号转换成电信号。
在一种可能实施的方式中，所述光纤探测装置包括：光纤连接器，所述光纤束插芯的管状件部分地插入所述光纤连接器的一端，以实现所述光纤连接器与所述光纤束插芯的多根所述光纤的光耦合；光收发器，被配置用于产生所述激发光、并接收所述发射光；以及图像传感器，被配置用于根据所述光收发器接受的所述发射光产生表征所述被测物体的图像的电信号。
在一种可能实施的方式中，所述光收发器包括：壳体，包括与所述光纤连接器光耦合的接口；光源，设置在所述壳体内，被配置用于产生光束；第一滤光片，设置在所述壳体内，被配置用于对来自所述光源的光束进行滤光，以产生所述激发光；以及光学转换组件，设置在所述壳体内，被配置用于将来自于所述第一滤光片的激发光引导至所述光纤束插芯、并将来自于所述光纤束插芯的发射光引导至所述图像传感器。
在一种可能实施的方式中，所述光学转换组件包括：二向色镜；物镜，设置在所述二向色镜和所述光纤连接器之间，所述物镜设置成接收由二向色镜反射的来自于所述第一滤光片的激发光以及来自于所述光纤束插芯的发射光、并进一步将所述激发光入射到所述光纤束插芯；目镜，所述目镜设置成接受由二向色镜透射的来自于所述物镜的发射光；以及第二滤光片，被配置用于对来自于所述目镜的发射光进行滤光，并将经过滤的发射光引导至所述图像传感器。
在一种可能实施的方式中，所述光纤束插芯包括：多根光纤；所述管状件，多根所述光纤的第二端部被保持在所述管状件中；以及固定部，所述光纤的中间部分固定在所述固定部中，所述光纤的第一端部从所述固定部伸出，以插入到待测目标。
在一种可能实施的方式中，所述多通道光纤记录系统还包括：信号采集装置，被构造成接收来自于所述光纤探测装置的电信号；以及换向装置，耦接于光纤探测装置与信号采集装置之间，以避免由于被测目标的运动导致的线缆缠绕。
根据本公开上述实施例的光纤束插芯的制作方法及多通道光纤记录系统，所制造的光纤束插芯的集成度高，体积小，重量轻，光纤束插芯的多根光纤的相对位置可自由设置，因此同时记录不同脑区具有较高的灵活性。
附图说明
图1为根据本公开的一种实施例的光纤束插芯的制作方法的流程图；
图2为根据本公开的一种实施例的多通道光纤记录系统的示意图；
图3为根据本公开实施例的多通道光纤记录系统的一种应用场景的示意图；
图4为根据本公开实施例的多通道光纤记录系统的另一种应用场景的示意图；以及
图5为根据本公开的一种实施例的光纤束插芯的制造方法的操作过程示意图；以及
图6为图5所示的操作过程示意图中采用准直板对光纤预定位及未对光纤预定位所造成光纤的角度偏差的对比图。
【附图中符号说明】
1—光纤连接器；2—管状件；3—固定部；4—光纤；5—光源；6—聚光透镜；7—第一滤光片；8—二向色镜；9—物镜；10—目镜；11—第二滤光片；12—图像传感器；13—壳体；14—光纤束插芯；15—光收发器；16—第一换向装置；17—信号采集装置；18—第二换向装置；19—光纤定位模具；以及20—光纤准直板。
具体实施方式
以下，将参照附图来描述本公开的实施例。但是应该理解，这些描述只是示例性的，而并非要限制本公开的范围。在下面的详细描述中，为便于解释，阐述了许多具体的细节以提供对本公开实施例的全面理解。然而，明显地，一个或多个实施例在没有这些具体细节的情况下也可以被实施。此外，在以下说明中，省略了对公知结构和技术的描述，以避免不必要地混淆本公开的概念。
在此使用的术语仅仅是为了描述具体实施例，而并非意在限制本公开。在此使用的术语“包括”、“包含”等表明了所述特征、步骤、操作和/或部件的存在，但是并不排除存在或添加一个或多个其他特征、步骤、操作或部件。
在此使用的所有术语(包括技术和科学术语)具有本领域技术人员通常所理解的含义，除非另外定义。应注意，这里使用的术语应解释为具有与本说明书的上下文相一致的含义，而不应以理想化或过于刻板的方式来解释。
在使用类似于“A、B和C等中至少一个”这样的表述的情况下，一般来说应该按照本领域技术人员通常理解该表述的含义来予以解释(例如，“具有A、B和C中至少一个的系统”应包括但不限于单独具有A、单独具有B、单独具有C、具有A和B、具有A和C、具有B和C、和/或具有A、B、C的系统等)。
根据本公开的一种方面的总体上的发明构思，提供一种光纤束插芯的制造方法，包括操作S100～操作S600。
本公开的一些实施例中，操作S100包括：制造光纤定位模具，在所述光纤定位模具上形成多个定位孔。
本公开的一些实施例中，操作S200包括：将多根光纤的第一端部分别插入光纤定位模具的定位孔中。
本公开的一些实施例中，操作S300包括：对裸露在外的多根光纤的临近光纤定位模具的部位利用固化材料与光纤定位模具进行固定，以保持多根光纤插入光纤定位模具一端的相对位置不变。
本公开的一些实施例中，操作S400包括：将多根光纤的第二端部插进管状件内，使得位于所述管状件和光纤定位模具之间的光纤形成伞状部。
本公开的一些实施例中，操作S500包括：利用固化材料固定所述伞状部和部分所述管状件，以形成固定部。
本公开的一些实施例中，操作S600包括：将插入所述光纤定位模具中的光纤从所述光纤定位模具抽出。
本公开的一些实施例中，操作S200包括：
步骤S210：制造光纤准直板，在光纤准直板上设置有多个与光纤定位模具上的定位孔的位置及数量一致的、被配置用于光纤穿过的准直孔，准直孔为通孔。
步骤S220：将光纤准直板平行放置于光纤定位模具的上方，并在光纤定位模具的正投影方向上、使每个准直孔与一个定位孔的位置相对应。
步骤S230：将多根光纤的第一端部分别穿过相对应的准直孔，以对光纤进行预定位。
步骤S240：将每个光纤的第一端部分别插入定位孔内。
本公开的一些实施例中，操作S300还包括：对裸露在外的多根光纤的临近光纤定位模具的部位利用固化材料与光纤定位模具进行固定，以保持多根光纤插入光纤定位模具一端的相对位置不变之后，移除光纤准直板。
本公开的一些实施例中，形成于光纤定位模具上的定位孔包括但不限于盲孔或变径孔。
在一种示意性的实施例中，光纤定位模具上形成多个盲孔中，至少两个盲孔的深度不同。
详细地，光纤定位模具上形成多个盲孔的深度均不相同，以满足光纤由固定部伸出的长度要求为宜。
在另一种示意性的实施例中，光纤定位模具上形成多个变径孔。
详细地，每个变径孔的孔径由开口位置沿深度方向减小。
进一步的，变径孔的开口位置的孔径大于或等于光纤的直径，以满足光纤通过为宜，变径孔的内部的孔径小于光纤的直径，以限制光纤伸入至光纤定位模具内的适合深度为宜。
根据本公开的另一方面的总体上的发明构思，提供一种多通道光纤记录系统，包括：
至少一个根据上述光纤束插芯的制造方法制造的光纤束插芯，所述光纤束插芯的露出所 述固定部的光纤插入被测目标，以向所述被测目标传导激发光并收集被测目标受激发后产生的发射光；以及光纤探测装置，与光纤束插芯连接，被配置用于产生激发光、接收发射光以及将光信号转换成电信号。
根据本公开实施例的光纤束插芯的制作方法及多通道光纤记录系统，多根光纤之间的空间位置可根据待测目标自由设置，提高了同时记录不同待测目标的灵活性。
图2为根据本公开的一种实施例的多通道光纤记录系统的示意图。
如图2所示，本公开实施例的多通道光纤记录系统包括光纤束插芯14和与光纤束插芯14连接的光纤探测装置，光纤束插芯14以向被测目标传导激发光并收集被测目标受激发后产生的发射光；光纤探测装置被配置用于产生激发光、接收发射光以及将光信号转换成电信号。
本公开的一些实施例中，光纤束插芯14包括：多根光纤4，多根光纤4的第一端部(图2中的下端)插入被测目标(例如被测动物的脑部)中；管状件2，多个所述光纤4的第二端部(图2中的上端)被保持在所述管状件2中；固定部3，所述光纤4的中间部分固定在所述固定部中，所述光纤4的第一端部从所述固定部3伸出，以插入到待测目标。
本公开可选的一些实施例中，光纤4的第一端部之间的空间位置根据待测目标的不同而自由设置。
本公开的一些实施例中，所述光纤4的第一端部从所述固定部3伸出，以插入到待测目标，例如插入动物头部不同的靶向脑区内。
本公开可选的一些实施例中，所述多根光纤4可以为塑料光纤或者其他材质光纤。
本公开的一些实施例中，固定部3对多根所述光纤4中间部分进行固定，以保证多根所述光纤4的第一端部相对空间位置不变。
本公开的一些实施例中，固定部3为固化材料对多根所述光纤4中间部分固化后形成。
在本实施例中，固化材料可以为牙科流体树脂、光固化胶水、环氧树脂、硅橡胶、PDMS、琼脂糖。
本公开可选的一些实施例中，固化材料采用具有遮光性能的材质。
本公开的一些实施例中，多根光纤4紧密排列，管状件2将所述多根所述光纤4的第二端部集中固定，多根所述光纤4的第二端部被保持在所述管状件中。
在本实施例中，根据多根光纤4的数量和直径，选择内径合适的管状件2。管状件2可为管状件或其他固定结构。
本公开的一些实施例中，管状件2为硬质毛细管，可选的，例如管状件2为不锈钢毛细管。
本公开的一些实施例中，所述多根光纤4包含6根不同长度的250微米直径的塑料光纤，靶向6个脑区，固定部3高7毫米，固定部3可为锥形或其他形状，管状件2高2毫米。
本公开可选的一些实施例中，所述光纤束插芯14还包括参考光纤，所述参考光纤不插入待测目标，仅反射激发光，作为多根光纤4中光信号的对照。
本公开可选的一些实施例中，参考光纤可以为一根或者多根。
本公开可选的一些实施例中，多根光纤4环绕参考光纤呈同心圆、方形、长方形或其它形状紧密排列。
本公开的一个实施例中，参考光纤为1根250微米直径的塑料光纤，多根光纤4围绕参考光纤呈同心圆紧密排列。
在本实施例中，光纤4第一端部一端的参考光纤端面设置有金属漆涂层，以便于增强参考光纤中的光信号。
光纤探测装置包括：光纤连接器1，所述光纤束插芯的管状件2部分地插入所述光纤连接器的一端，以实现所述光纤连接器1与所述光纤束插芯14的多根所述光纤4的光耦合；光收发器15，被配置用于产生所述激发光、并接收所述发射光；以及图像传感器12，被配置用于根据所述光收发器15接受的所述发射光产生表征所述被测物体的图像的电信号。
本公开可选的一些实施例中，光纤探测装置的重量可以为小于2克。
在本公开的一个实施例中，光纤探测装置15尺寸为7×7×16毫米，重0.8克。
本公开可选的一些实施例中，光纤连接器1为管状连接件，所述光纤束插芯14的管状件2部分地插入所述光纤连接器1的一端，以实现所述光纤连接器1与所述光纤束插芯14的多根所述光纤4的光耦合。
本公开的一个实施例中，光纤连接器1外形尺寸为3×3×3毫米的管状连接件，其对侧各有1个M0.6的微型螺丝，被配置用于与光收发器15固定。
本公开的一个实施例中，光收发器15包括：壳体13，包括与所述光纤连接器1光耦合的接口；光源5，设置在所述壳体13内，被配置用于产生光束；第一滤光片7，设置在所述壳体13内，被配置用于对来自所述光源5的光束进行滤光，以产生所述激发光；以及光学转换组件，设置在所述壳体13内，被配置用于将来自于所述第一滤光片7的激发光引导至所述光纤束插芯14、并将来自于所述光纤束插芯14的发射光引导至所述图像传感器12。
本公开的一个实施例中，壳体13包括与所述光纤连接器1光耦合的接口，并且壳体13的内表面具有不平整结构，以减少光收发器15内的杂散光。壳体13遮光度大于90％。在本公开的一个实施例中，壳体13可为3D打印黑色塑料或其他遮光材料制成。
在本公开的一个实施例中，光源5为一个微型LED，尺寸为1.7×1.3×0.4毫米，设置于 壳体13内壁的右下侧。所述微型LED发出蓝色的光束，主波长为470纳米。光源5还可以为两个主波长不同的微型LED，发出具有两个主波长的双色光束。光源5还可以为多个主波长不同的微型LED。
本公开的一个实施例中，第一滤光片7设置于光源5远离壳体13内壁一侧，长宽均不大于5毫米。在本公开的一个实施例中，第一滤光片7为直径为2毫米的圆片，厚1毫米，波长选择范围为460-480纳米，OD值至少为6。
本公开可选的一些实施例中，光源5与第一滤光片7之间还可以设置聚光透镜6，以集中收集光源5产生的光束。所述聚光透镜6直径不大于5毫米。在本公开的一个实施例中，聚光透镜6为直径2毫米的半球透镜，所述半球透镜平面一端紧贴的设置在光源5的左侧。第一滤光片7竖直设置在聚光透镜6的左侧，与聚光透镜6高点相距0.1毫米。
光学转换组件包括：二向色镜8；物镜9，设置在所述二向色镜8和所述光纤连接器1之间，所述物镜9设置成接收由二向色镜8反射的来自于所述第一滤光片7的激发光以及来自于所述光纤束插芯14的发射光、并进一步将所述激发光入射到所述光纤束插芯14；目镜10，所述目镜10设置成接受由二向色镜8透射的来自于所述物镜9的发射光；以及第二滤光片11，被配置用于对来自于所述目镜10的发射光进行滤光，并将经过滤的发射光引导至所述图像传感器12。
在本公开的一个实施例中，光学转换组件与设置于第一滤光片7远离光源5一侧。二向色镜8上端向右倾斜的与第一滤光片7相邻设置，物镜9设置于二向色镜8下方、目镜10设置于二向色镜8上方，第二光滤光片11设置于目镜10上方。
所述二向色镜8反射透射面上端倾斜地设置于第一滤光片7的另一侧，与第一滤光片7成竖直45°夹角，宽度不大于5毫米，被配置用于反射激发光及透射发射光。
在本公开的一个实施例中，二向色镜8长为5.2毫米，宽为3毫米，厚为1.1毫米，中心波长500纳米，二向色镜8反射透射面上端向右倾斜与第一滤光片7成竖直45°夹角设置，二向色镜8中心点与第一滤光片7相距1.8毫米。
本公开可选的一些实施例中，所述物镜9在二向色镜8的下方，直径不大于5毫米。
在本公开的一个实施例中，物镜9为直径2毫米、焦距2毫米的双凸透镜，放置在二向色镜8的竖直下方，物镜9上表面最高点与二向色镜8反射透射面下表面的中心点相距1.6毫米，物镜9的中心点与的二向色镜8反射透射面中心点在竖直方向上重合。
本公开可选的一些实施例中，所述目镜10在二向色镜8的上方，直径不大于5毫米。
在本公开的一个实施例中，目镜10为直径3毫米，焦距6.3毫米的双凸透镜，放置在二向色镜8的上方。
本公开可选的一些实施例中，所述第二光滤光片11在目镜10上方，长宽均不大于5毫米。
在本公开的一个实施例中，第二光滤光片11为直径3.5毫米的圆片，厚1毫米，波长选择范围为515-535纳米，OD值至少为6，放置在目镜10的上方，与目镜10的上表面相距0.1毫米。
本公开可选的一些实施例中，图像传感器12设置在壳体13外远离光纤束插芯14一端，被配置用于根据所述光收发器15接受的所述发射光产生表征所述被测物体的图像的电信号。
在本公开的一个实施例中，图像传感器12为600线模拟CMOS，尺寸6.5×6.5×2毫米，位于第二光滤光片11的上方，与第二光滤光片11相距3.4毫米。
图3为根据本公开实施例的多通道光纤记录系统的一种应用场景的示意图。
为了记录待测目标的信号，如同时记录多个靶向脑区的信号，预先要将荧光探针，如钙活动指示剂GCaMP6s，注射进靶向脑区，同时将所述光纤4的第一端部插入靶向脑区。光源5发出的光束经聚光透镜6汇聚后被第一滤光片7过滤净化形成激发光。激发光被二向色镜8反射进物镜9，由光纤束插芯14传导至靶向脑区，以激发预先注射进靶向脑区的钙活动指示剂GCaMP6s。GCaMP6s受到激发后发出的绿色荧光信号作为发射光被光纤4的第一端部收集。发射光经过固定部3和管状件2被物镜9接收，经二向色镜8透射后被目镜10接收，再经第二滤光片11过滤净化，最后被图像传感器12以每秒25帧的频率采集。图像传感器12将所述发射光的光信号转换成电信号。
如图3所示，在一种实施例中，多通道光纤记录系统还可以包括信号采集装置17，信号采集装置17与光纤探测装置耦接，并被构造成接收来自于所述光纤探测装置的电信号。在本公开的一个实施例中，采集装置17为安装了模拟视频采集卡的电脑。
如图3所示，多通道光纤记录系统还可以包括耦接于光纤探测装置与采集装置17之间的换向装置，被配置用于避免动物运动导致的线缆缠绕。换向装置可为导电滑环或有源换向器，在本公开的一个实施例中，换向装置为一个第一换向装置16，所述第一换向装置16为直径6.5毫米，长10毫米的4路导电滑环。
图4为根据本公开实施例的多通道光纤记录系统的另一种应用场景的示意图。
如图4所示，在本公开的另一个实施例中，利用两个本公开实施例所述的多通道光纤记录系统对两个被测目标进行探测。换向装置还可以包括两个第一换向装置16和一个第二换向装置18，所述第二换向装置18为直径6.5毫米，长10毫米的8路导电滑环。
图1为根据本公开的一种实施例的光纤束插芯的制作方法的流程图。
如图1和5所示，本公开还提供了一种光纤束插芯14的制造方法，包括：
操作S100：制作光纤定位模具，在所述光纤定位模具上形成多个定位孔。
操作S200：将多根光纤4的第一端部分别插入光纤定位模具的定位孔中。
操作S300：对裸露在外的多根光纤4的临近光纤定位模具的部位利用固化材料与光纤定位模具进行固定，以保持多根光纤4插入光纤定位模具一端的相对位置不变。
操作S400：将多根光纤4的第二端部插进管状件2内，使得位于所述管状件2和光纤定位模具之间的光纤4形成伞状部。
操作S500：利用固化材料固定所述伞状部和部分所述管状件，以形成固定部3。
操作S600：将插入所述光纤定位模具中的光纤4从所述光纤定位模具抽出。
图5为根据本公开的一种实施例的光纤束插芯的制造方法的操作过程示意图。
图6为图5所示的操作过程示意图中采用准直板对光纤预定位及未对光纤预定位所造成光纤的角度偏差的对比图。
如图5所示，根据本公开的一个实施例，光纤束插芯14制造方法具体可以包括步骤S1-S14。
在步骤S1中，根据所需记录的靶向脑区数量、脑区大小和三维坐标，使用雕刻机或数控机床在动物颅骨模型的相应位置打深度、直径合适的定位孔，制作光纤定位模具。例如，动物颅骨模型可以为动物头部大小相当的硬质小块或者3D打印技术制作具有真实颅骨结构的1：1颅骨模型。本公开的一个实施例中，为在尺寸20×20×10毫米的透明亚克力块相应位置打6个合适深度、250微米直径的定位孔，制作光纤定位模具。
在步骤S2中，依据光纤定位模具19上的定位孔的位置及数量制造光纤准直板20，并使光纤准直板的孔径与定位孔的孔径一致。
在步骤S3中，打磨多根光纤4的第一端部分使其光滑无划痕。本公开的一个实施例中，分别使用1500目、3000目、7000目、10000目和12000目的砂纸打磨7根30毫米长、直径250微米的塑料光纤的一端,使其光滑无划痕。
在步骤S4中，将多根光纤4的第一端部插入光纤准直板20上设置的相对应的准直孔中，并使第一端部穿过准直孔。
在步骤S5中，将多根光纤4的第一端部插入所述定位孔中，直到遇到较大阻力为止，其余部分留在光纤定位模具外。本公开的一个实施例中，将7根光纤中的中6根插入光纤定位模具的定位孔中。
在步骤S6中，使用固化材料涂抹裸露在外的多根光纤4的临近光纤定位模具的部位。本公开的一个实施例中，使用黑色光固化牙科树脂涂抹光纤定位模具表面和6根光纤接触的区域，涂抹树脂厚度小于2毫米，使用波长450纳米的光照射使之固化，并在固化后向上移除光纤准直板20。
本公开可选的一些实施例中，可以在涂抹固化材料前使用分离剂涂抹光纤定位模具表面，以减少固化后取下的阻力。
在步骤S7中，根据多根光纤4的数量和直径，选择内径合适的管状件2，将多根光纤4留在光纤定位模具外的光纤4的第二端部插进管状件2内，使得位于所述管状件2和光纤定位模具之间的光纤4形成伞状部。本公开的一个实施例中，选用长2毫米，外径1.8毫米，内径0.8毫米的不锈钢毛细管，将多根光纤留在光纤定位模具外的光纤的第二端部插进不锈钢毛细管内约3毫米。
在步骤S8中，将参考光纤插入管状件2，使得所述光纤4布置在环绕所述参考光纤的同心圆、方形、长方形或其它形状上，并且所述参考光纤的一端靠近所述光纤定位模具。本公开的一个实施例中，将第7根光纤作为参考光纤，插入不锈钢毛细管，使其处在其余6根光纤的中心，7根光纤组成同心圆，第7根光纤插入不锈钢毛细管一端接近光纤定位模具表面。
在步骤S9中，使用反光涂料涂抹参考光纤靠近光纤定位模具的端面。本公开的一个实施例中，使用金属漆涂抹第7根光纤靠近光纤定位模具的端面。
在步骤S10中，将光纤4插入后的管状件2往光纤定位模具的方向推进，尽量靠近模具，直到遇到较大阻力为止，使得位于所述管状件2和光纤定位模具之间的光纤4形成伞状部；
在步骤S11中，使用固化材料固定所述伞状部和部分所述管状件2，以形成固定部3。本公开的一个实施例中，使用黑色光固化牙科树脂涂抹覆盖光纤定位模具外的伞状部以及部分管状件2，使用波长450纳米的光照射使其固化，形成锥形的固定部3。
在步骤S12中，将固化后的光纤束插芯14从光纤定位模具取下，用光纤切割刀切割掉露在管状件2外的光纤4部分。
本公开可选的一些实施例中，光纤束插芯14固化后，可以使用遮光涂料涂抹光纤束插芯14表面，进一步增强遮光性能。
在步骤S13中，打磨抛光管状件2端面，使管状件2内多根光纤4端面光洁无划痕。本公开的一个实施例中，分别使用1500目、3000目、7000目、10000目和12000目的砂纸打磨抛光管状件2裸露的端面。
在步骤S14中，将光纤束插芯14与光纤连接器1连接。例如，可以粘接剂固定连接。
如不设置参考光纤，则省略以上步骤6、步骤7。
这样的实施方式中，基于上述步骤S1至S14光纤束插芯的制作方法。如图6所示，在光纤数为6根的使用场景中，使用光纤准直板的制作方法相较于不使用光纤准直板的制作方法，在光纤插芯的过程中，光纤角度相对于90度的变化量更小(每根光纤测量两个正交方向的角度偏差，n＝12)，因此，光纤的准直度更高。使用单因素方法分析，显著性校验结果P-value< 0.01。
根据本公开的光纤束插芯的制造方法及多通道光纤记录系统，光纤束插芯中多根光纤相对位置可自由设置，同时靶向不同脑区，提高了记录不同脑区的灵活性；光纤束紧密排列，集成度高，因此体积小，重量轻，可以被被测目标随身携带；采用换向装置避免线缆变形导致的信号失真，也避免了被测目标活动导致的线缆缠绕问题，可以进行数小时甚至多天的连续记录。
还需要说明的是，实施例中提到的方向用语，例如“上”、“下”、“前”、“后”、“左”、“右”等，仅是参考附图的方向，并非用来限制本公开的保护范围。贯穿附图，相同的元素由相同或相近的附图标记来表示。在可能导致对本公开的理解造成混淆时，将省略常规结构或构造。并且图中各部件的形状和尺寸不反映真实大小和比例，而仅示意本公开实施例的内容。
说明书与权利要求中所使用的序数例如“第一”、“第二”、“第三”等的用词，以修饰相应的元件，其本身并不意味着该元件有任何的序数，也不代表某一元件与另一元件的顺序、或是制造方法上的顺序，该些序数的使用仅用来使具有某命名的一元件得以和另一具有相同命名的元件能做出清楚区分。
以上所述的具体实施例，对本公开的目的、技术方案和有益效果进行了进一步详细说明，所应理解的是，以上所述仅为本公开的具体实施例而已，并不被配置用于限制本公开，凡在本公开的精神和原则之内，所做的任何修改、等同替换、改进等，均应包含在本公开的保护范围之内。

Claims (13)

  1. 一种光纤束插芯的制造方法，包括：
    制造光纤定位模具，在所述光纤定位模具上形成多个定位孔；
    将多根光纤的第一端部分别插入光纤定位模具的定位孔中；
    对裸露在外的多根光纤的临近光纤定位模具的部位利用固化材料与光纤定位模具进行固定，以保持多根光纤插入光纤定位模具一端的相对位置不变；
    将多根光纤的第二端部插进管状件内，使得位于所述管状件和光纤定位模具之间的光纤形成伞状部；
    利用固化材料固定所述伞状部和部分所述管状件，以形成固定部；以及
    将插入所述光纤定位模具中的光纤从所述光纤定位模具抽出。
  2. 根据权利要求1所述的光纤束插芯的制造方法，所述将多根光纤的第一端部分别插入光纤定位模具的定位孔中，包括：
    制造光纤准直板，在所述光纤准直板上设置有多个与所述光纤定位模具上的定位孔的位置及数量一致的、被配置用于光纤穿过的准直孔；
    将所述光纤准直板平行放置于所述光纤定位模具的上方，并在所述光纤定位模具的正投影方向上、使每个所述准直孔与一个所述定位孔的位置相对应；
    将多根所述光纤的第一端部分别穿过相对应的所述准直孔，以对所述光纤进行预定位；以及
    将每个所述光纤的第一端部分别插入所述定位孔内；
    其中，所述准直孔为通孔。
  3. 根据权利要求2所述的光纤束插芯的制造方法，其中，在对裸露在外的多根光纤的临近光纤定位模具的部位利用固化材料与光纤定位模具进行固定，以保持多根光纤插入光纤定位模具一端的相对位置不变之后，还包括移除所述光纤准直板。
  4. 根据权利要求1至3中任一项所述的光纤束插芯的制造方法，其中，形成于所述光纤定位模具上的所述定位孔包括盲孔或变径孔。
  5. 根据权利要求1至3中任一项所述的光纤束插芯的制造方法，其中，在所述将多根光纤的第二端部插进管状件内之后，还包括：
    在所述多根光纤中插入一根或多根参考光纤，使得多根光纤环绕参考光纤紧密排列成同心圆、方形、长方形或其它形状，并且所述参考光纤的一端靠近所述光纤定位模具。
  6. 根据权利要求1至3中任一项所述的光纤束插芯的制造方法，其中，所述固化材料具 有遮光性能。
  7. 根据权利要求1至3中任一项所述的光纤束插芯的制造方法，其中，在所述将插入所述光纤定位模具中的光纤从所述光纤定位模具抽出之后，还包括：
    切除所述多根光纤的露出所述管状件的部分。
  8. 一种多通道光纤记录系统，包括：
    至少一个根据权利要求1至7中的任一项所述的光纤束插芯的制造方法制造的光纤束插芯，所述光纤束插芯的露出所述固定部的光纤插入被测目标，以向所述被测目标传导激发光并收集被测目标受激发后产生的发射光；以及
    光纤探测装置，与光纤束插芯连接，被配置用于产生激发光、接收发射光以及将光信号转换成电信号。
  9. 根据权利要求8所述的多通道光纤记录系统，其中，所述光纤探测装置包括：
    光纤连接器，所述光纤束插芯的管状件部分地插入所述光纤连接器的一端，以实现所述光纤连接器与所述光纤束插芯的多根所述光纤的光耦合；
    光收发器，被配置用于产生所述激发光、并接收所述发射光；以及
    图像传感器，被配置用于根据所述光收发器接受的所述发射光产生表征所述被测物体的图像的电信号。
  10. 根据权利要求9所述的多通道光纤记录系统，其中，所述光收发器包括：
    壳体，包括与所述光纤连接器光耦合的接口；
    光源，设置在所述壳体内，被配置用于产生光束；
    第一滤光片，设置在所述壳体内，被配置用于对来自所述光源的光束进行滤光，以产生所述激发光；以及
    光学转换组件，设置在所述壳体内，被配置用于将来自于所述第一滤光片的激发光引导至所述光纤束插芯、并将来自于所述光纤束插芯的发射光引导至所述图像传感器。
  11. 根据权利要求10所述的多通道光纤记录系统，其中，所述光学转换组件包括：
    二向色镜；
    物镜，设置在所述二向色镜和所述光纤连接器之间，所述物镜设置成接收由二向色镜反射的来自于所述第一滤光片的激发光以及来自于所述光纤束插芯的发射光、并进一步将所述激发光入射到所述光纤束插芯；
    目镜，所述目镜设置成接受由二向色镜透射的来自于所述物镜的发射光；以及
    第二滤光片，被配置用于对来自于所述目镜的发射光进行滤光，并将经过滤的发射光引导至所述图像传感器。
  12. 根据权利要求8至11中的任一项所述的多通道光纤记录系统，其中，所述光纤束插芯包括：
    多根光纤；
    所述管状件，多根所述光纤的第二端部被保持在所述管状件中；以及
    固定部，所述光纤的中间部分固定在所述固定部中，所述光纤的第一端部从所述固定部伸出，以插入到待测目标。
  13. 根据权利要求12所述的多通道光纤记录系统，还包括：
    信号采集装置，被构造成接收来自于所述光纤探测装置的电信号；以及
    换向装置，耦接于光纤探测装置与信号采集装置之间，以避免由于被测目标的运动导致的线缆缠绕。
PCT/CN2022/123828 2021-10-22 2022-10-08 光纤束插芯的制造方法及多通道光纤记录系统 WO2023066029A1 (zh)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103443679A (zh) * 2011-03-09 2013-12-11 古河电气工业株式会社 光连接器、多芯光纤和光纤束结构的调芯方法以及光纤排列转换部件
CN109581592A (zh) * 2018-07-16 2019-04-05 深圳朗光科技有限公司 光纤合束器的制备装置及制备方法
CN110384471A (zh) * 2019-08-02 2019-10-29 上海长征医院 基于阵列光纤束照明成像的生物组织检测系统
US20190384024A1 (en) * 2017-03-07 2019-12-19 Corning Optical Communications LLC Optical fiber ferrules incorporating a glass faceplate and methods of fabricating the same
CN212723461U (zh) * 2020-08-27 2021-03-16 武汉富基科技有限公司 光纤连接器组件以及光收发模块组件
CN213309653U (zh) * 2020-09-14 2021-06-01 北京脑科学与类脑研究中心 一种荧光信号全光纤记录系统

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL9101173A (nl) * 1990-11-09 1992-06-01 Novem Optical Fibers Bv Werkwijze en inrichting voor het vervaardigen van lichtpanelen.
NL9301585A (nl) * 1993-03-23 1994-10-17 Tchai Lights Bv Werkwijze en inrichting voor het vervaardigen van lichtpanelen, alsmede een dergelijk lichtpaneel.
JP3899842B2 (ja) * 2001-05-01 2007-03-28 富士ゼロックス株式会社 光配線回路積層体および光電気配線装置
US7348587B2 (en) * 2001-06-28 2008-03-25 Fujifilm Corporation Method for producing biochemical analysis data and apparatus used therefor
CN106599594B (zh) * 2016-12-22 2019-08-13 白云绮 基于太赫兹量子波信息的人体数字化模型及疾病诊疗系统
CN110989073B (zh) * 2019-12-23 2020-12-22 哈尔滨工程大学 一种高能激光光纤束及其制作方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103443679A (zh) * 2011-03-09 2013-12-11 古河电气工业株式会社 光连接器、多芯光纤和光纤束结构的调芯方法以及光纤排列转换部件
US20190384024A1 (en) * 2017-03-07 2019-12-19 Corning Optical Communications LLC Optical fiber ferrules incorporating a glass faceplate and methods of fabricating the same
CN109581592A (zh) * 2018-07-16 2019-04-05 深圳朗光科技有限公司 光纤合束器的制备装置及制备方法
CN110384471A (zh) * 2019-08-02 2019-10-29 上海长征医院 基于阵列光纤束照明成像的生物组织检测系统
CN212723461U (zh) * 2020-08-27 2021-03-16 武汉富基科技有限公司 光纤连接器组件以及光收发模块组件
CN213309653U (zh) * 2020-09-14 2021-06-01 北京脑科学与类脑研究中心 一种荧光信号全光纤记录系统

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