WO2023041042A1 - Dispositif d'extraction et de détection d'acide nucléique et procédé d'extraction et de détection d'acide nucléique - Google Patents

Dispositif d'extraction et de détection d'acide nucléique et procédé d'extraction et de détection d'acide nucléique Download PDF

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Publication number
WO2023041042A1
WO2023041042A1 PCT/CN2022/119336 CN2022119336W WO2023041042A1 WO 2023041042 A1 WO2023041042 A1 WO 2023041042A1 CN 2022119336 W CN2022119336 W CN 2022119336W WO 2023041042 A1 WO2023041042 A1 WO 2023041042A1
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WIPO (PCT)
Prior art keywords
nucleic acid
acid extraction
assembly
driving
base
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PCT/CN2022/119336
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English (en)
Chinese (zh)
Inventor
戴立忠
解亚平
龙泽宇
杜鹃
陈琦
符诚
Original Assignee
圣湘生物科技股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority claimed from CN202122258839.3U external-priority patent/CN216155875U/zh
Priority claimed from CN202111678985.XA external-priority patent/CN114317230B/zh
Priority claimed from CN202123429987.3U external-priority patent/CN216738297U/zh
Priority claimed from CN202221043103.2U external-priority patent/CN217265654U/zh
Application filed by 圣湘生物科技股份有限公司 filed Critical 圣湘生物科技股份有限公司
Publication of WO2023041042A1 publication Critical patent/WO2023041042A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/24—Apparatus for enzymology or microbiology tube or bottle type
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters

Definitions

  • the present application relates to the technical field of biological detection, and more specifically, to a nucleic acid extraction and detection device and a nucleic acid extraction and detection method.
  • nucleic acid detection is the nucleic acid of the virus. Nucleic acid detection is to find out whether there is nucleic acid of foreign invading virus in the patient's respiratory samples, blood or feces to determine whether they are infected by the virus. Therefore, once the nucleic acid is detected as "positive", it can prove that there is a virus in the patient's body.
  • Nucleic acid detection needs to extract the nucleic acid first, and then detect the extracted nucleic acid.
  • the nucleic acid detection equipment on the market is generally a semi-automatic instrument, which needs to be manually inserted into the extracted nucleic acid, and then quantitatively analyzed by the instrument. Manual intervention leads to large test errors and slow test speed.
  • nucleic acid extraction and detection equipment that integrates nucleic acid extraction and detection (such as the Chinese patent application with application number 201811093908.6), nucleic acid extraction and nucleic acid detection are integrated. When detecting nucleic acids, it cannot be flexibly adapted to work, which has certain limitations.
  • a nucleic acid extraction and detection device and a nucleic acid extraction and detection method are provided.
  • a nucleic acid extraction and detection device comprising a nucleic acid extraction device and a nucleic acid detection device that are independent of each other;
  • the nucleic acid extraction device comprises:
  • the nucleic acid extraction mechanism, carrying mechanism, first pipetting mechanism and clamping mechanism all installed on the first installation frame; the carrying mechanism is used to carry the first kit; the first pipetting mechanism is used to absorb The nucleic acid extracted by the nucleic acid extraction mechanism is amplified in the first kit carried by the carrying mechanism;
  • the nucleic acid detection device includes:
  • the transfer mechanism includes a transfer plate for carrying the first kit, and the transfer plate can be relatively
  • the second mounting rack is switched between the first position and the second position; when the transfer plate is at the first position, the transfer mechanism is used to transfer the first reagent box on the transfer plate to the On the nucleic acid testing institution;
  • the transfer plate when the nucleic acid extraction device and the nucleic acid detection device are docked with each other, the transfer plate can extend into the first installation frame when it is in the second position, and the clamping mechanism can clamp and carry the The first kit on the carrying mechanism is transferred to the transfer plate.
  • the nucleic acid extraction device further includes a first reagent carrier installed on the first installation frame, the first reagent carrier is used to store the first reagent tube, and the first reagent tube is used for Storage of nucleic acid reaction reagents;
  • the first pipetting mechanism is used to suck the nucleic acid reaction reagent in the first reagent tube into the first reagent box carried by the carrying mechanism.
  • the nucleic acid extraction device further includes a first reagent kit carrier installed on the first installation rack for storing the first reagent kit; the clamping mechanism can clamp and store the first reagent kit The first kit on the first kit carrier is placed on the carrying mechanism.
  • the nucleic acid extraction device further includes a sealing film carrier and a sealing film mechanism installed on the first installation frame, the sealing film carrier is used to store the sealing film; the sealing film mechanism It is used to absorb the sealing film stored on the sealing film carrier, and is used to seal the sealing film on the first kit carried on the carrying mechanism.
  • the nucleic acid extraction device further includes a sample holder installed on the first mounting frame, and the clamping mechanism is used to clamp the first kit carried on the carrying mechanism Leave the sample on the sample holder.
  • the nucleic acid extraction device also includes a sample loading mechanism, a clamping mechanism, a sample transfer mechanism, and a second pipetting mechanism all installed on the first mounting frame;
  • the sample loading mechanism is used for Store the sampling tube;
  • the sample transfer mechanism is used to transfer the sample tube between the sample loading mechanism and the clamping mechanism;
  • the clamping mechanism is used to clamp the sampling tube, and the sample transfer mechanism is also used to The sampling tube clamped in the clamping mechanism is uncapped or capped;
  • the second pipetting mechanism is used to suck the sample in the sampling tube clamped in the clamping mechanism and transfer it to the nucleic acid extraction mechanism place.
  • the nucleic acid extraction device further includes a second reagent carrier installed on the first mounting frame, the second reagent carrier is used to store the second reagent tube, and the second reagent tube is stored Reagents for nucleic acid extraction;
  • the second pipetting mechanism is used to suck the reagent for nucleic acid extraction in the second reagent tube to the nucleic acid extraction mechanism.
  • the nucleic acid extraction device further includes a tip carrier installed on the first installation frame, and the tip carrier is used to store the tip;
  • the first pipetting mechanism can pick up the gun tip on the gun tip carrier, the first pipetting mechanism can absorb the nucleic acid extracted by the nucleic acid extraction mechanism through the gun tip, and release the nucleic acid to the first in the kit.
  • the nucleic acid extraction mechanism includes a bearing assembly, the bearing assembly includes a bearing seat, the bearing seat is used to carry the second kit, and the first pipetting mechanism is used to absorb the second kit.
  • the nucleic acid in the nucleic acid is amplified to the first kit carried by the carrying mechanism;
  • the nucleic acid extraction device also includes a second kit carrier mounted on the first installation rack for storing a second kit;
  • the clamping mechanism can clamp the second reagent box stored on the second reagent box carrier to the bearing seat.
  • the nucleic acid extraction mechanism includes an automatic loading magnetic rod cover assembly
  • the automatic loading magnetic rod cover assembly includes:
  • a base assembly including a base
  • the magnetic rod cover assembly includes a first assembly frame that is drivingly connected to the drive device, and the drive device is used to drive the first assembly frame to move in a first direction;
  • a magnetic bar assembly comprising a second assembly frame drivingly connected to the driving device and a magnetic bar mounted on the second assembly frame, the driving device is used to drive the second assembly frame along the first direction move;
  • the bearing base, the first assembly frame and the second assembly frame are sequentially arranged along the first direction
  • the magnetic bar cover assembly further includes a magnetic bar that can be positioned on the second reagent box sleeve, the magnetic bar sleeve includes a mating block and a sleeve body connected to the mating block;
  • the first assembly frame has a matching mechanism, and driven by the driving device, the first assembly frame can move along the first direction to the position where the matching mechanism is connected to the matching block; when the matching mechanism is connected to the matching block, driven by the driving device, the second The assembly frame can drive the magnetic bar to insert or withdraw from the sleeve body.
  • the matching mechanism includes an elastic resisting piece
  • the first assembly bracket has an assembly groove for accommodating the mating block, the side wall of the assembly groove is provided with an installation hole, the The elastic resisting piece is installed in the installation hole, and the matching block has a matching recess;
  • the elastic abutting piece has a retracted state allowing the mating block to enter and exit the fitting groove and an ejected state where it abuts and fits with the mating recess;
  • the first assembly bracket has a through hole communicating with the assembly groove.
  • the elastic abutting member abuts and fits with the fitting recess, driven by the driving device, the second assembly
  • the frame can drive the magnetic rod to insert or withdraw from the sleeve body through the through hole.
  • the fitting block further has a limiting protrusion, and the limiting protrusion is arranged on opposite sides of the fitting concave portion in a third direction perpendicular to the first direction, so as to Cooperating with the elastic resisting piece, the matching block is limited along the third direction.
  • the elastic resisting member includes a mounting sleeve, a first elastic member and a ball, the mounting sleeve is passed through the mounting hole, and the first elastic member and the ball are disposed on the mounting hole. Under the pushing action of the first elastic member, a part of the ball protrudes from the installation sleeve toward one end of the fitting groove, so as to abut and fit with the mating recess .
  • the length of the magnetic rod is greater than the depth of the sleeve body, so that the second assembly frame can push the magnetic rod against the sleeve body when it is inserted into the sleeve body. until the matching block is separated from the matching mechanism.
  • the driving device includes a base, a first moving base, a first driving assembly, a second moving base and a second driving assembly;
  • the base is arranged on the base, the first moving seat and the second moving seat are both movably connected to the base along the first direction;
  • the first driving assembly is arranged on the on the base, and is drivingly connected with the first moving base;
  • the second driving assembly is arranged on the first moving base, and is drivingly connected with the second moving base;
  • the first assembly frame is connected with the first moving base, and the second assembly frame is connected with the second moving base.
  • the first driving assembly includes a first screw, a first driving member and a first screw nut;
  • the first screw rod is rotatably connected to the base around its own axis, and the axial direction of the first screw rod is parallel to the first direction;
  • the first driving member is installed on the base, and It is drivingly connected with the first screw;
  • the first screw nut is threadedly connected to the first screw, and fixedly connected with the first moving seat.
  • the second driving assembly includes a second screw, a second driving member and a second screw nut;
  • the second screw rod is rotatably connected to the first moving seat around its own axis, and the axial direction of the second screw rod is parallel to the first direction; the second driving member is installed on the The first moving seat is connected to the second screw by driving; the second screw nut is threaded on the second screw and fixedly connected to the second moving seat.
  • the first moving base is provided with a first slider
  • the second moving base is provided with a second slider
  • the base is provided with a The first slide rail; the first slide block and the second slide block are both slidingly engaged with the first slide rail.
  • the base assembly further includes a third drive assembly
  • the base is movably connected to the base along a second direction perpendicular to the first direction, and the third driving assembly is arranged on the base and is drivingly connected to the base.
  • heating blocks are provided on the bearing seat corresponding to the lysis station and the elution station of the second reagent kit; each of the heating blocks has a first installation hole , an electric heating element is arranged in the first installation hole.
  • each of the heating blocks has a second installation hole, and a temperature protection switch is arranged in the second installation hole; the electric heating element is electrically connected to an external power supply, and the temperature protection switch is electrically connected connected to the circuit between the electric heating element and the external power supply; when the temperature of the heating block is higher than a preset value, the temperature protection switch disconnects the electric heating element from the external power supply electrical connection;
  • Each of the heating blocks has a third installation hole, and a temperature sensor is arranged in the third installation hole.
  • the carrying seat is movably connected to the base along a third direction perpendicular to the first direction
  • the carrying assembly further includes a fourth driving assembly arranged on the base , the fourth driving assembly is drivingly connected to the bearing base, so as to drive the bearing base to move along the third direction to a warehouse-in position located below the magnetic rod assembly and the magnetic rod cover assembly.
  • the nucleic acid extraction mechanism further includes a casing assembly, the casing assembly includes a casing arranged on the base, the driving device, the magnetic rod cover assembly and the magnetic rod assembly are all located within said housing;
  • the shell has an inlet and outlet for the bearing seat to enter and exit along the third direction;
  • the shell assembly also includes a door rotatably arranged on the base, and the door can be positioned relative to the base.
  • the seat rotates to a closed position for closing the inlet and outlet and an open position for opening the inlet and outlet;
  • the nucleic acid extraction mechanism includes an extraction vibration component, and the extraction vibration component includes:
  • a mounting plate having first and second sides opposite in a first direction
  • a first mounting seat used for mounting the magnetic rod cover, and the first mounting seat is movably connected to the mounting plate along a second direction perpendicular to the first direction;
  • the first driving assembly includes a first driving unit and a first transmission structure both arranged on the mounting plate, and the first driving unit is transmission-connected to the first mounting base through the first transmission structure;
  • a second mounting seat used for mounting a magnetic bar
  • the second mounting seat is movably connected to the mounting plate along the second direction, and is arranged with the first mounting seat along the second direction;
  • the second driving assembly includes a second driving unit and a second transmission structure both arranged on the mounting plate, and the second driving unit is transmission-connected to the second mounting base through the second transmission structure;
  • first transmission structure is located on the first side
  • second transmission structure is located on the second side
  • the first driving unit is installed on the second side, and the output shaft of the first driving unit penetrates from the second side to the first side, and is connected to the first side a transmission structure transmission connection;
  • the second driving unit is installed on the first side, and the output shaft of the second driving unit passes through from the first side to the second side, and is in driving connection with the second transmission structure.
  • the first transmission structure includes a first driving pulley, a first driven pulley and a first synchronous belt
  • the first driving pulley is installed on the output shaft of the first driving unit
  • the first driven pulley is rotatably connected to the first side, and is spaced apart from the first driving pulley along the second direction
  • the first synchronous belt is sheathed on the first Between a driving pulley and the first driven pulley, and fixedly connected with the first mounting seat.
  • the second transmission structure includes a second driving pulley, a second driven pulley and a second synchronous belt
  • the second driving pulley is installed on the output shaft of the second driving unit
  • the second driven pulley is rotatably connected to the second side, and is spaced apart from the second driving pulley along the second direction
  • the second synchronous belt is sheathed on the first Between the two driving pulleys and the second driven pulley, they are fixedly connected with the second mounting seat.
  • the extracting vibration assembly further includes a first guide rail, a first slider, a second slider, a first transmission plate and a second transmission plate;
  • the first guide rail is fixedly connected to the mounting plate and extends lengthwise along the second direction, the first sliding member and the second sliding member are both slidably fitted on the first guide rail, and the first sliding member
  • a mounting base is fixedly connected to the first sliding member, and the second mounting base is fixedly connected to the second sliding member;
  • the first transmission plate is fixedly connected to the first sliding member, and is in transmission connection with the first transmission structure
  • the second transmission plate is fixedly connected to the second sliding member, and is in transmission connection with the second transmission structure. Structural drive connections.
  • the extracting vibration assembly further includes a support base and a third drive assembly, the mounting plate is movably connected to the support base along the first direction, and the third drive assembly is installed on The support seat is connected with the installation plate in transmission.
  • the extraction vibration assembly further includes a bottom plate, the mounting plate is fixedly connected to the bottom plate, the bottom plate is movably connected to the support base along the first direction, and is connected to the The third driving assembly is transmission-connected.
  • the extraction vibration assembly further includes a second guide rail and a third sliding member, the second guide rail is fixedly connected to the support seat, and extends lengthwise along the first direction, and the first guide rail
  • the three sliding parts are fixedly connected to the bottom plate, and are slidably matched with the second guide rail.
  • the third drive assembly includes a third drive unit and a third transmission structure, and the third transmission structure includes a third driving pulley, a third driven pulley, and a third synchronous belt;
  • the third drive unit is installed on the support base, the third driving pulley is installed on the output shaft of the third drive unit, and the third driven pulley is rotatably connected to the support base, and arranged at intervals along the first direction with the third driving pulley, the third synchronous belt is sleeved between the third driving pulley and the third driven pulley, and is connected to the Bottom plate fixed connection.
  • the nucleic acid extraction mechanism includes:
  • a heating module which is movably installed on the installation frame in the first direction, and the heating module is used to install the second reagent box;
  • a first drive module installed on the installation frame, connected to the heating module, and capable of driving the heating module to reciprocate relative to the installation frame in the first direction;
  • the magnetic rod sleeve carrier is movably installed on the installation frame in the second direction, and the magnetic rod sleeve carrier is used to install the magnetic rod sleeve assembly; the magnetic rod sleeve carrier can be moved in the second direction move relative to the installation frame to drive the magnetic bar set assembly to be inserted into the reaction well of the second reagent box or to be removed from the reaction well; the magnetic bar set assembly is in the first direction It is detachably connected with the magnetic bar sleeve carrier;
  • the second reagent box drives and plugs into the The magnetic rod cover assembly in the reaction well moves to separate from the magnetic rod cover carrier.
  • the magnetic bar cover carrier is provided with a sliding slot extending in the first direction, and the sliding slot is used for slidingly fitting with the magnetic bar cover assembly.
  • the magnetic rod sleeve carrier includes at least two sliding slots arranged at intervals in the third direction, and the first direction, the second direction and the third direction intersect two by two. .
  • the first driving module includes a first driving motor, a screw and a screw nut, the screw is connected to the first driving motor, and the screw nut is connected to the heating module , the screw rod is in transmission connection with the screw nut.
  • one of the installation frame and the heating module is provided with a first track, and the other is provided with a first guide groove; the heating module passes through the first track and the first guide groove.
  • a guide slot fits to be slidably connected with the installation frame in the first direction.
  • the heating module is detachably connected to the installation frame.
  • the heating module includes a heating body and at least one first heating strip and at least one second heating strip arranged on the heating body, the first heating strip and the second heating strip In the third direction, the first heating strip and the second heating strip extend along the first direction; the first direction, the second direction and the third direction intersect two by two;
  • the first heating strip is used for pyrolysis heating
  • the second heating strip is used for elution heating
  • the nucleic acid extraction mechanism further includes a magnetic rod carrier, which is movably installed on the installation frame in the second direction and located above the magnetic rod sleeve carrier, the magnetic rod Rod carrier for mounting magnetic rods;
  • the magnetic rod carrier can move relative to the installation frame in the second direction, so as to drive the magnetic rod to be plugged into the magnetic rod sleeve of the magnetic rod sleeve assembly or to be removed from the magnetic rod out of the sleeve.
  • the nucleic acid extraction mechanism further includes a second driving module installed on the installation frame, and the second driving module is connected with the magnetic rod cover carrier to drive the magnetic rod cover the carrier reciprocates relative to the mounting frame in the second direction;
  • the nucleic acid extraction mechanism also includes a third drive module installed on the installation frame, the third drive module is connected with the magnetic rod carrier to drive the magnetic rod carrier in the second direction reciprocating relative to the mounting frame.
  • the installation frame includes a frame body and a frame mounting plate, the heating module, the first driving module, and the frame mounting plate are all installed on the frame body, and the magnetic bar cover Both the carrier and the magnetic rod carrier are movably mounted on the frame mounting plate in the second direction;
  • the nucleic acid extraction mechanism also includes a fourth drive module arranged on the frame body, the fourth drive module is connected to the frame mounting plate to drive the frame mounting plate in a third position relative to the frame body.
  • a nucleic acid extraction and detection method using the above-mentioned nucleic acid extraction and detection equipment comprising the steps of:
  • the nucleic acid extraction mechanism performs nucleic acid extraction, and the first pipetting mechanism absorbs the nucleic acid extracted by the nucleic acid extraction mechanism to amplify in the first kit carried by the carrying mechanism;
  • the clamping mechanism clamps the first kit carried on the carrying mechanism to the transfer plate
  • the transfer plate of the transfer mechanism is switched from the second position to the first position
  • the transfer mechanism transfers the first kit carried on the transfer plate to the nucleic acid detection mechanism, and the nucleic acid detection mechanism performs nucleic acid detection.
  • Fig. 1 is an axonometric view of nucleic acid extraction and detection equipment provided by an embodiment of the present invention
  • Fig. 2 is another axonometric view of the nucleic acid extraction and detection equipment shown in Fig. 1;
  • Figure 3 is a rear view of the nucleic acid extraction and detection device shown in Figure 1;
  • Figure 4 is a front view of the nucleic acid extraction and detection device shown in Figure 1;
  • Fig. 5 is another axonometric view of the nucleic acid extraction and detection device shown in Fig. 1;
  • Fig. 6 is the axonometric view of the nucleic acid extraction mechanism of the first embodiment of the present application (housing is hidden);
  • Figure 7 is a side view of the nucleic acid extraction mechanism shown in Figure 6 (housing is hidden);
  • Figure 8 is a front view of the nucleic acid extraction mechanism shown in Figure 6;
  • Fig. 9 is a side view of the nucleic acid extraction mechanism of the nucleic acid extraction and detection device shown in Fig. 1;
  • Figure 10 is a top view of the nucleic acid extraction mechanism shown in Figure 9;
  • Fig. 11 is an exploded view of the heating block of the bearing assembly of the nucleic acid extraction mechanism shown in Fig. 9;
  • Fig. 12 is a structural schematic diagram of the magnetic rod cover assembly and the magnetic rod assembly of the nucleic acid extraction mechanism shown in Fig. 6;
  • Fig. 13 is a schematic structural view of the magnetic rod cover assembly shown in Fig. 12;
  • Fig. 14 is a schematic diagram of the cooperation structure between the elastic supporting member and the mating block of the magnetic rod cover assembly shown in Fig. 13;
  • FIG. 15 is a schematic structural view of the magnetic rod cover of the magnetic rod cover assembly shown in FIG. 13;
  • Fig. 16 is a front view of the magnetic rod cover shown in Fig. 15;
  • Fig. 17 is a top view of the magnetic rod cover shown in Fig. 15;
  • Fig. 18 is a schematic structural diagram of the extraction and oscillation component of the nucleic acid extraction instrument in the second embodiment of the present application.
  • Fig. 19 is a schematic structural diagram of the extraction oscillation component shown in Fig. 18 under another viewing angle;
  • Fig. 20 is a schematic structural diagram of the nucleic acid extractor in the second embodiment of the present application (the magnetic rod cover is inserted into the second kit);
  • Fig. 21 is a schematic structural view of the nucleic acid extractor in the second embodiment of the present application (the magnetic rod cover is inserted into the second kit, and the magnetic rod is inserted into the magnetic rod cover);
  • Fig. 22 is a schematic diagram of the structure of the nucleic acid extractor in the second embodiment of the present application (the magnetic rod is inserted into the magnetic rod cover, and the magnetic rod cover exits the second kit);
  • Figure 23 is a structural diagram of the nucleic acid extraction mechanism provided in the third embodiment of the present application.
  • Figure 24 is a partial structural diagram of the nucleic acid extraction mechanism shown in Figure 23;
  • Figure 25 is another partial structural diagram of the nucleic acid extraction mechanism shown in Figure 23;
  • Figure 26 is another partial structural diagram of the nucleic acid extraction mechanism shown in Figure 23;
  • Figure 27 is an enlarged view at A of the structure shown in Figure 26;
  • Fig. 28 is a structural diagram of the second kit carrier of the second kit of the nucleic acid extraction mechanism shown in Fig. 23;
  • Fig. 29 is a structural part of another viewing angle of the structure shown in Fig. 24;
  • Fig. 30 is a structural part of another viewing angle of the structure shown in Fig. 24;
  • Figure 31 is a structural diagram of the magnetic rod cover assembly of the nucleic acid extraction mechanism shown in Figure 1;
  • nucleic acid extraction mechanism 2510, installation frame; 2511, frame body; 25111, frame bottom plate; 25112, installation part; 2512, frame mounting plate; 25121, first frame mounting plate; 25122, second frame mounting plate; Heating module; 2521, heating body; 2522, first heating strip; 2523, second heating strip; 2524, heating storage tank; 2530, first driving module; 2531, first driving motor; 2532, screw rod; 2533, Screw nut; 2541, reaction hole; 2542, second kit carrier; 25421, placement slot; 25422, weight reduction hole; 2550, magnetic bar sleeve carrier; 2551, chute; 2552, socket; 2560, magnetic Rod carrier; 2570, third magnetic rod cover assembly; 2571, assembly department; 2572, third magnetic rod cover; 2580, third magnetic rod; 2590, first track; 25120, second drive module; 251201, second Drive motor; 251202, the first driving wheel; 251203, the first driven wheel; 251204, the first transmission belt; 25130, the third driving module; 251301, the
  • Carrying mechanism 40. First pipetting mechanism; 42. First transfer seat; 50. Clamping mechanism; 60. Sample loading mechanism; 70. Clamping mechanism; 80. Sample transfer mechanism; 90, the second pipetting mechanism; 92, the second transfer seat; 110, the first waste area; 120, the tip carrier; 130, the second waste area; 140, the second reagent carrier; 150, the first Kit carrier; 160, second kit carrier; 170, first reagent carrier; 180, sealing film carrier; 190, sealing film mechanism; 240, retention sample carrier; 300, nucleic acid detection device; 301, 302, transfer mechanism; 3021, transfer plate; 303, nucleic acid detection mechanism; 304, transfer mechanism; a, first kit; b, second kit; d, sampling tube.
  • first and second are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features.
  • the features defined as “first” and “second” may explicitly or implicitly include at least one of these features.
  • “plurality” means at least two, such as two, three, etc., unless otherwise specifically defined.
  • a first feature being "on” or “under” a second feature may mean that the first and second features are in direct contact, or that the first and second features are indirect through an intermediary. touch.
  • “above”, “above” and “above” the first feature on the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature.
  • “Below”, “beneath” and “beneath” the first feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is less horizontally than the second feature.
  • an embodiment of the present invention provides a nucleic acid extraction and detection device 100 , which includes a nucleic acid extraction device 200 and a nucleic acid detection device 300 that are independent of each other.
  • the nucleic acid extraction device 200 and the nucleic acid detection device 300 can be used alone, or they can be used together by docking.
  • Nucleic acid extraction device 200 comprises the first installation rack 10, nucleic acid extraction mechanism, carrying mechanism 30 (referring to Fig. 2), the first pipetting mechanism 40 and clamping mechanism 50 (referring to Fig. 3 and Fig. 4), nucleic acid extraction mechanism, carrying mechanism 30. Both the first pipetting mechanism 40 and the clamping mechanism 50 are installed on the first installation frame 10 .
  • the nucleic acid extraction mechanism is used to extract nucleic acid.
  • the carrying mechanism 30 is used to carry the first reagent box a (refer to FIG. 5 ), which can contain reagents for reacting with nucleic acid, so as to facilitate the amplification reaction between nucleic acid and the above reagents for nucleic acid detection.
  • the first kit a is a 24-well deep-well plate.
  • the first pipetting mechanism 40 is used to suck the nucleic acid extracted by the nucleic acid extraction mechanism into the first kit a carried by the carrying mechanism 30, so as to undergo an amplification reaction with the reagent contained in the first kit a to facilitate nucleic acid detection.
  • the clamping mechanism 50 is used for clamping the first reagent box a carried on the carrying mechanism 30 on other mechanisms.
  • the clamping mechanism 50 can clamp the first kit a carried on the carrying mechanism 30 to the nucleic acid detection device 300, so that the nucleic acid detection device 300 can The nucleic acid after performing the amplification reaction is detected.
  • the clamping mechanism 50 can also clamp the first kit a carried on the carrying mechanism 30 to other mechanisms, which is not limited here.
  • the nucleic acid detection device 300 includes a second mounting frame 301, a transfer mechanism 302, a nucleic acid detection mechanism 303, and a transfer mechanism 304, and the transfer mechanism 302, the nucleic acid detection mechanism 303, and the transfer mechanism 304 are all installed on the second mounting frame 301 on.
  • the transfer mechanism 302 includes a transfer plate 3021 (see FIG. 1 ), and the transfer plate 3021 can switch between the first position and the second position relative to the second mounting frame 301. In the second position, the transfer plate 3021 can Extend the second mounting bracket 301 to interface with the external structure.
  • the transfer plate 3021 when the nucleic acid extraction device 200 is docked with the nucleic acid detection device 300, at the second position, the transfer plate 3021 can extend out of the second mounting frame 301 and into the first mounting frame 10, so as to Docking with the clamping mechanism 50 of the nucleic acid extraction device 200 , at this time, the clamping mechanism 50 can clamp the first reagent box a carried on the carrying mechanism 30 to the transfer plate 3021 .
  • the transfer plate 3021 When in the first position, the transfer plate 3021 is retracted into the second mounting frame 301, and the transfer mechanism 304 is used to transfer the first kit a located on the transfer plate 3021 to the nucleic acid detection mechanism 303 for detection.
  • the nucleic acid extraction and detection device 100 provided in the embodiment of the present invention includes a nucleic acid extraction device 200 and a nucleic acid detection device 300.
  • the nucleic acid extraction device 200 and the nucleic acid detection device 300 can be docked.
  • the nucleic acid extraction and detection device 100 since the nucleic acid extraction and detection device 100 provided in this embodiment, the nucleic acid extraction device 200 and the nucleic acid detection device 300 are set independently of each other, it is more flexible and has a wide range of applications to meet the occasions for separate nucleic acid extraction and nucleic acid detection.
  • the first mounting frame 10 and the second mounting frame 301 may or may not be in contact.
  • the nucleic acid extraction device 200 includes multiple nucleic acid extraction mechanisms, and the multiple nucleic acid extraction mechanisms can perform nucleic acid extraction independently of each other. In this way, part or all of the nucleic acid extraction mechanisms can be flexibly selected to perform nucleic acid extraction operations separately according to specific conditions, which is conducive to improving the nucleic acid extraction efficiency of the equipment on the one hand; on the other hand, it is conducive to improving the flexibility and compatibility of the equipment during actual use. .
  • the nucleic acid extraction device 200 includes three nucleic acid extraction mechanisms, and the three nucleic acid extraction mechanisms are arranged at intervals in the first direction. It should be understood that, in other embodiments, the nucleic acid extraction device may also include other numbers of nucleic acid extraction mechanisms, which are not limited herein.
  • the first embodiment of the present application provides a nucleic acid extraction mechanism 210
  • the nucleic acid extraction mechanism 210 includes an automatic loading magnetic rod cover assembly and a carrier assembly 219
  • the automatic loading magnetic rod cover assembly includes The base assembly, the driving device, the first magnetic rod cover assembly 216 and the first magnetic rod assembly 217 .
  • the base assembly includes a base 2181 on which the drive unit is mounted.
  • the first magnetic rod cover assembly 216 includes a first assembly frame 2161 drivingly connected with the driving device.
  • the driving device is used to drive the first assembly frame 2161 to move along the third direction.
  • the first magnetic rod cover assembly 216 includes a second assembly frame 2171 drivingly connected with the driving device and a first magnetic rod 2172 mounted on the second assembly frame 2171 .
  • the driving device is used to drive the second assembly frame 2171 to move along the third direction.
  • the carrying assembly 219 includes a carrying seat 2191 for carrying the second reagent kit b, and the carrying seat 2191, the first assembly frame 2161 and the second assembly frame 2171 are sequentially arranged along the third direction.
  • the first magnetic rod cover assembly 216 further includes a first magnetic rod cover 2162 that can be positioned on the second reagent box b.
  • the first magnetic rod cover 2162 includes a matching block 21621 and a cover body 21622 connected with the matching block 21621 .
  • the first assembly frame 2161 has a matching mechanism, and driven by the driving device, the first assembly frame 2161 can move along the third direction to a position where the matching mechanism is connected to the mating block 21621 .
  • the second assembly frame 2171 can drive the first magnetic bar 2172 to insert or withdraw from the sleeve body 21622 .
  • the first magnetic bar cover 2162 is initially positioned on the second kit b, and the second kit b is loaded on the bearing seat 2191 .
  • the driving device drives the first assembly frame 2161 to move toward the second reagent box b along the third direction until the matching mechanism on the first assembly frame 2161 is in contact with the second reagent
  • the mating block 21621 of the first magnetic rod cover 2162 on the box b is connected.
  • the driving device drives the first assembly frame 2161 to move away from the second reagent box b along the third direction, so that the first magnetic bar cover 2162 moves along with the first assembly frame 2161 and separates from the second reagent box b.
  • the first magnetic rod cover 2162 of the first magnetic rod cover assembly 216 and the first magnetic rod 2172 of the first magnetic rod assembly 217 cooperate to extract nucleic acid from the sample in the second kit b.
  • the first assembly frame 2161 is driven by the driving device, thereby realizing the connection between the matching mechanism on the first assembly frame 2161 and the matching block 21621 of the first magnetic bar cover 2162 on the second kit b, that is, realizing the first magnetic
  • the automatic picking of the rod cover 2162 does not require manual assembly of the first magnetic rod cover 2162, which reduces labor costs, avoids human pollution, and is beneficial to improving detection accuracy.
  • the matching mechanism includes an elastic resisting member 21611 , and the first assembly frame 2161 has a matching slot 21612 for receiving the matching block 21621 .
  • the side wall 21613 of the fitting groove 21612 defines an installation hole, and the elastic resisting member 21611 is installed in the installation hole.
  • the mating block 21621 has a mating concave portion 21623 capable of abutting against the elastic resisting member 21611 .
  • the elastic resisting member 21611 has a receiving state allowing the mating block 21621 to enter and exit the mating groove 21612 and an ejecting state where it abuts and fits with the mating recess 21623 .
  • the driving device drives the first assembly frame 2161 to move towards the second reagent box b, and the matching block 21621 of the first magnetic rod cover 2162 gradually enters the matching groove 21612,
  • the elastic abutting piece 21611 is in a shrinking state under the pushing action of the mating block 21621, until the mating block 21621 is in place, and the elastic abutting piece 21611 pops out to abut and fit with the mating recess 21623 on the mating block 21621 (At this moment, the driving device stops driving the first assembly frame 2161 to continue to move), so as to limit the mating block 21621 in the mating groove 21612, that is, to realize the connection between the first magnetic rod cover 2162 and the first assembly frame 2161. Then, the driving device drives the first assembly frame 2161 to move away from the second reagent box b along the third direction and reset, so as to prepare for nucleic acid extraction.
  • the first assembly frame 2161 has a through hole communicating with the mating groove 21612.
  • the second assembly frame 2171 can drive the first magnetic rod 2172 to insert or withdraw from the sleeve body 21622 through the through hole, so that the first magnetic rod 2172 and the first magnetic rod sleeve 2162 cooperate with each other to realize nucleic acid extraction .
  • the mating block 21621 also has a limiting protrusion 21624, and the limiting protrusion 21624 is arranged on opposite sides of the mating concave portion 21623 in the second direction perpendicular to the third direction, so as to resist against the elastic
  • the component 21611 cooperates with the mating block 21621 to limit the position along the second direction, that is, to prevent the mating block 21621 from moving relative to the first assembly frame 2161 along the second direction.
  • the mating block 21621 can be provided with a plurality of mating recesses 21623 arranged at intervals along the second direction, and the first assembly frame 2161 is also provided with a plurality of elastic abutting pieces 21611, and the plurality of elastic abutting pieces 21611 and the plurality of elastic abutting pieces 21611
  • the matching recesses 21623 are arranged in one-to-one correspondence.
  • a plurality of elastic abutting pieces 21611 and a plurality of mating recesses 21623 are abutted and matched one by one to jointly limit the mating block 21621 in the mating groove 21612, so that the mating block 21621 is more firmly limited in position Fitting slot 21612.
  • both sides of the mating block 21621 in the first direction can be provided with mating recesses 21623, and correspondingly, both side walls 21613 of the mating groove 21612 in the first direction are provided with elastic abutting pieces 21611, thereby further Improve the stability of the mating block 21621 limited in the mating groove 21612.
  • each fitting concave portion 21623 can be provided with limiting protrusions 21624 on both sides in the second direction, so as to cooperate with each elastic resisting piece 21611 to limit the fitting block 21621 along the second direction.
  • the elastic resisting member 21611 includes a mounting sleeve, a first elastic member and a ball.
  • the installation sleeve passes through the installation hole on the side wall 21613 of the matching groove 21612 .
  • Both the first elastic member and the ball are arranged in the installation sleeve, and under the push action of the first elastic member, a part of the ball protrudes from the installation sleeve toward one end of the fitting groove 21612 to abut against the fitting recess 21623 Matching, so that the matching block 21621 is limited in the matching groove 21612.
  • the first elastic member may be a spring
  • the ball may be a steel ball, which are not limited herein.
  • the length of the first magnetic bar 2172 is greater than the depth of the sleeve body 21622, so that the second assembly frame 2171 can drive the first magnetic bar 2172 into the sleeve body 21622, and can push the sleeve body 21622 to the matching Block 21621 is detached from the mating mechanism.
  • the driving device drives the first assembly frame 2161 to move towards the second reagent box b until the cover body 21622 of the first magnetic bar cover 2162 is inserted into the second reagent box b.
  • the driving device drives the second assembly frame 2171 to move toward the second reagent box b, so that the first magnetic rod 2172 on the second assembly frame 2171 is inserted into the sleeve body 21622, and as the second assembly frame 2171 continues to move toward the second reagent box
  • the box b moves, and the first magnetic bar 2172 is inserted into the bottom of the cover body 21622, and then the first magnetic bar cover 2162 is pushed away from the first assembly frame 2161 and repositioned on the second reagent box b (i.e., to realize the removal of the first test kit b).
  • Magnetic Rod Cover 2162 Magnetic Rod Cover 2162).
  • the mating block 21621 squeezes the elastic abutting member 21611, so that the elastic abutting member 21611 shrinks (that is, is in a contracted state), so that the mating block 21621 gradually withdraws from the mating Groove 21612 until completely disengaged.
  • the driving device includes a base 211 , a first moving base 213 , a first driving assembly 212 , a second moving base 215 and a second driving assembly 214 .
  • the base 211 is disposed on the base 2181 , and the first moving base 213 and the second moving base 215 are both movably connected to the base 211 along the third direction.
  • the first driving assembly 212 is disposed on the base 211 and is drivingly connected with the first moving base 213 to drive the first moving base 213 to move along the third direction.
  • the second driving assembly 214 is disposed on the first moving base 213 and is drivingly connected with the second moving base 215 to drive the second moving base 215 to move relative to the first moving base 213 along the third direction.
  • the first assembly frame 2161 of the first magnetic bar set assembly 216 is connected to the first moving base 213 , so that the first magnetic bar set assembly 216 and the first moving base 213 move together.
  • the second assembly frame 2171 of the first magnetic bar assembly 217 is connected to the second moving base 215 , so that the first magnetic bar assembly 217 and the second moving base 215 move together.
  • the third direction is the up-down direction.
  • this article takes the third direction as an up-and-down direction as an example for illustration.
  • the first driving assembly 212 drives the first moving base 213 to descend relative to the base 211 (that is, down along the third direction), and the second driving assembly 214 drives the second moving base 215 to rise relative to the first moving base 213, so that the first The moving base 213 drives the first magnetic rod cover assembly 216 to descend relative to the base 211 , while the first magnetic rod assembly 217 on the second moving base 215 does not descend relative to the base 211 .
  • the first driving assembly 212 drives the first moving base 213 to rise relative to the base 211
  • the second driving assembly 214 drives the second moving base 215 descends relative to the first moving base 213 , so that the first moving base 213 drives the first magnetic rod cover assembly 216 to rise relative to the base 211 , while the first magnetic rod assembly 217 on the second moving base 215 does not rise relative to the base 211 .
  • the second drive assembly 214 drives the second moving base 215 to rise or fall relative to the first moving base 213, while the first A driving assembly 212 does not drive the first moving base 213 to move relative to the base 211 , so that the second moving base 215 drives the first magnetic rod assembly 217 to rise or fall relative to the base 211 .
  • the first drive assembly 212 drives the first moving base 213 to rise or fall
  • the second driving assembly 214 does not drive the second moving base 215 rises or falls
  • the first moving seat 213 drives the first magnetic rod cover assembly 216 to rise or fall
  • the first magnetic rod set assembly 216 and the first magnetic rod assembly 217 can be independently moved along the third direction (that is, ascending or descending) through the driving device, and both can also be realized to move synchronously along the third direction. Then complete the nucleic acid extraction.
  • first magnetic rod assembly 217 and the first magnetic rod cover assembly 216 are required to move synchronously along the third direction, only the first driving assembly 212 is required to drive the first moving seat 213 to move along the third direction, and it is not necessary to The synchronous drive of the two driving components as in the prior art is required, which greatly reduces the design difficulty of the hardware and software of the nucleic acid extraction mechanism 210, reduces the equipment cost, and also ensures that the first magnetic field does not occur during the synchronous movement.
  • the excessive movement of the rod 2172 relative to the first magnetic rod sleeve 2162 eliminates potential safety hazards.
  • the first driving assembly 212 includes a first screw 2121 , a first driving member 2122 and a first screw nut 2123 .
  • the first threaded rod 2121 is rotatably connected to the base 211 around its own axis, and the axial direction of the first threaded rod 2121 is parallel to the third direction.
  • the first driving member 2122 is installed on the base 211 and is drivingly connected with the first screw rod 2121 , so that the first driving member 2122 can drive the first screw rod 2121 to rotate around its own axis.
  • the first screw nut 2123 is threadedly connected to the first screw rod 2121 , so that when the first screw rod 2121 rotates, the first screw nut 2123 can be driven to move along the axial direction of the first screw rod 2121 (ie, the third direction). Moreover, the first screw nut 2123 is fixedly connected to the first moving base 213 , so that the first moving base 213 can move together with the first screw nut 2123 . In this way, when the first magnetic bar set assembly 216 needs to rise or fall relative to the base 211 , the first driving member 2122 drives the first screw rod 2121 to rotate around its own axis, thereby driving the first screw nut 2123 along the direction of the first screw rod 2121 .
  • the first screw nut 2123 drives the first moving seat 213 to move in the third direction, and then the first magnetic rod cover assembly 216 follows the first moving seat 213 and moves relative to the base 211 in the third direction.
  • the movement means that the first magnetic rod cover assembly 216 rises or falls relative to the base 211 .
  • the first driving member 2122 may be a motor.
  • the first driving assembly 212 uses a screw pair formed by the first screw 2121 and the first screw nut 2123 to drive the first moving seat 213 to move, which has a simple structure and high motion accuracy. And easy to control.
  • the first driving assembly 212 may also adopt other driving structures, such as a belt drive structure, as long as it can drive the first moving base 213 to move along the third direction, and there is no limitation here.
  • the second driving assembly 214 includes a second screw 2141 , a second driving member 2142 and a second screw 2141 nut.
  • the second screw rod 2141 is rotatably connected to the first moving base 213 around its own axis, and the axial direction of the second screw rod 2141 is parallel to the third direction.
  • the second driving member 2142 is installed on the first moving base 213 and is drivingly connected with the second screw rod 2141 , so that the second driving member 2142 can drive the second screw rod 2141 to rotate around its own axis.
  • the nut of the second threaded rod 2141 is threadedly connected to the second threaded rod 2141 , so that when the second threaded rod 2141 rotates around its own axis, the nut of the second threaded rod 2141 can be driven to move along the third direction relative to the first moving seat 213 .
  • the nut of the second screw rod 2141 is fixedly connected with the second moving base 215 , so that the second moving base 215 can move along the third direction relative to the first moving base 213 along with the nut of the second screw rod 2141 .
  • the second driving member 2142 drives the second screw rod 2141 to rotate around its own axis, thereby driving the nut of the second screw rod 2141 to move along the second screw rod.
  • 2141 moves in the axial direction (that is, the third direction)
  • the second screw rod 2141 nut drives the second moving seat 215 to move relative to the first moving seat 213 along the third direction
  • the first magnetic bar assembly 217 follows the second moving seat 215 together Moving relative to the first moving base 213 along the third direction realizes that the first magnetic bar assembly 217 rises or falls relative to the first moving base 213 .
  • the second driving member 2142 may be a motor.
  • the second drive assembly 214 uses a screw pair formed by the second screw 2141 and the nut of the second screw 2141 to drive the second moving base 215 to move relative to the first moving base 213, the structure Simple, high motion precision and easy to control.
  • the second driving assembly 214 can also adopt other driving structures, such as a belt drive structure, as long as it can drive the second moving base 215 to move relative to the first moving base 213 along the third direction, here Not limited.
  • the first sliding block 2131 is provided on the first moving base 213
  • the second sliding block 2151 is provided on the second moving base 215
  • the base 211 is provided with a first sliding rail 2111 extending longitudinally along the third direction
  • the first sliding block 2131 and the second sliding block 2151 are both slidingly engaged with the first sliding rail 2111 .
  • the movement of the first moving base 213 along the third direction is guided by the sliding cooperation between the first sliding block 2131 and the first sliding rail 2111 , so that the movement of the first moving base 213 is more stable and reliable.
  • the movement of the second moving base 215 along the third direction is guided by the sliding cooperation between the second sliding block 2151 and the first sliding rail 2111 , so that the movement of the second moving base 215 is stable and reliable.
  • the first sliding rail 2111 may include two, the two first sliding rails 2111 are arranged oppositely, and the first moving seat 213 and the second moving seat 215 are located between the two first sliding rails 2111 .
  • the first moving seat 213 is respectively provided with first sliders 2131 facing the two sides of the first slide rails 2111, and each first slider 2131 is slidably matched with the corresponding first slide rails 2111 to further enhance the first slide rail 2111.
  • the second moving seat 215 is respectively provided with second sliding blocks 2151 facing the two sides of the two first sliding rails 2111, and each second sliding block 2151 is slidably matched with the corresponding first sliding rails 2111 to further enhance the The guiding effect of the second movable seat 215.
  • the base assembly further includes a third driving assembly 2182 .
  • the base 211 is movably connected to the base 2181 along a first direction perpendicular to the third direction.
  • the third driving component 2182 is disposed on the base 2181 and is drivingly connected with the base 211 to drive the base 211 to move along the first direction.
  • the third driving assembly 2182 is used to drive the base 211 to move along the first direction, and then drive the first magnetic bar set assembly 216 and the first magnetic bar assembly 217 to move together along the first direction, thereby realizing the transfer of magnetic beads etc. between different test tubes. transfer.
  • the base 2181 is provided with a second slide rail (not shown) extending lengthwise along the first direction
  • the base 211 is provided with a third slide block (shown in FIG. Not marked), utilize the sliding cooperation between the third slide block and the second slide rail to guide the movement of the base 211 relative to the base 2181 along the first direction, so that the movement of the base 211 is more stable and reliable.
  • the multiple second slide rails are arranged at intervals along the second direction perpendicular to the third direction and the first direction, and the second slide rails are parallel to each other.
  • a plurality of third sliding blocks corresponding to the plurality of second sliding rails are disposed on the base 211 , and each third sliding block is slidingly matched with a corresponding second sliding rail. In this way, the movement of the base 211 relative to the base 2181 is simultaneously guided by a plurality of second sliding rails and third sliding blocks, thereby improving the guiding effect.
  • the first direction, the second direction and the third direction are perpendicular to each other.
  • the first direction is the X direction in FIG. 1
  • the second direction is the Y direction in FIG. 1
  • the third direction is the Z direction in FIG. 1 .
  • the third driving assembly 2182 includes a third driving member 21821 , a driving pulley 21822 , a driven pulley (not shown in the figure) and a transmission belt 21823 .
  • the third driving member 21821 is mounted on the base 2181 , and the driving pulley 21822 is drive-connected to the output shaft of the third driving member 21821 , so that the third driving member 21821 can drive the driving pulley 21822 to rotate.
  • the driven pulley is rotatably connected to the base 2181 and arranged at intervals with the driving pulley 21822 along the first direction.
  • the driving belt 21823 is sleeved between the driving pulley 21822 and the driven pulley, and is fixedly connected to the base 211, so that the driving belt 21823 can be driven sequentially between the driving pulley 21822 and the driven pulley when the driving pulley 21822 rotates.
  • the transmission belt 21823 drives the base 211 to move along the first direction.
  • the third driving assembly 2182 drives the base 211 to move along the first direction by means of belt transmission, which has a simple structure and low cost.
  • the third driving member 21821 may be a motor.
  • the third driving assembly 2182 is not limited to the belt transmission method.
  • the third driving assembly 2182 can also adopt the screw pair, that is to say, the third driving assembly 2182 includes a third wire Rod, the third driver 21821 and the third screw nut.
  • the third screw rod is rotatably connected to the base 2181 around its own axis, and the axis of the third screw rod is parallel to the first direction.
  • the third driving member 21821 is installed on the base 2181 and is drivingly connected with the third screw rod, so that the third driving member 21821 can drive the third screw rod to rotate around its own axis.
  • the third screw nut is threadedly connected to the third screw rod, so that when the third screw rod rotates, the third screw rod nut can be driven to move along the first direction (ie, the axial direction of the third screw rod). Moreover, the third screw nut is fixedly connected to the base 211 , so that the base 211 moves along the first direction along with the third screw nut. In this way, the third driving assembly 2182 uses a screw pair to drive the base 211 to move relative to the base 2181 along the first direction, which has a simple structure, high motion precision and is easy to control.
  • the third driving member 21821 may be a motor.
  • the second kit b has at least a cleavage station for cleavage, a washing station for washing and purification, and a washing station for elution.
  • the unloading station, the lysis station is provided with a lysis tube for containing the lysate, the washing station is provided with a washing tube for containing the washing buffer, and the elution station is provided with an elution tube for containing the eluent.
  • the first drive assembly 212 and the second drive assembly 214 are used to drive the first magnetic rod cover assembly 216 and the first magnetic rod assembly 217 to move in the third direction to enter or withdraw from the reagent tube of the second reagent box b, and draw Or release the magnetic beads, and also achieve vibration to mix the reagents in the reagent tube.
  • the carrier assembly 219 further includes a heating block 2192, and the positions on the carrier seat 2191 respectively corresponding to the lysis station and the elution station of the second kit b are provided with the heating block 2192.
  • Each heating block 2192 is used to heat the reagents in the corresponding lysis tube or elution tube, so as to ensure the smooth progress of lysis reaction and elution reaction, and improve the efficiency and accuracy of nucleic acid extraction.
  • one cracking tube or a row of cracking tubes can be set in the cracking station.
  • the elution station can be provided with one elution tube or a row of elution tubes.
  • the number of heating blocks 2192 is equal to the number of cracking stations and elution stations.
  • the second reagent kit b includes two cracking stations and two elution stations, and the number of heating blocks 2192 is four, of which two One heating block 2192 heats the reagents in the lysis tubes of the two cleavage stations respectively, and the other two heating blocks 2192 heat the reagents in the elution tubes of the two elution stations respectively.
  • each heating block 2192 is formed with a heating groove 21921 for accommodating the bottom of the lysis tube or the elution tube, so that when the second kit b is loaded on the carrier 2191, the lysis station of the second kit b
  • the bottom of each cracking tube is respectively accommodated in the corresponding heating tank 21921, and the bottom of each eluting tube is also respectively accommodated in the corresponding heating tank 21921, thereby heating the lysate in the cracking tube and the eluent in the eluting tube .
  • the amount of reagents to be added in the lysis tube is relatively large, while the amount of reagents to be added in the elution tube is relatively small, so that the liquid level in the lysis tube is high and the amount of reagents in the elution tube is relatively high. Liquid level is low.
  • the depth of the heating tank 21921 for accommodating the lysis tube is greater than the depth of the heating tank 21921 for accommodating the elution tube, so that The depth of the heating tank 21921 for accommodating the lysis tube matches the liquid level of the lysate in the lysis tube, and the depth of the heating tank 21921 for accommodating the elution tube matches the liquid level of the eluent in the elution tube.
  • each heating block 2192 has a first mounting hole 21922 .
  • the first installation hole 21922 is provided with an electric heating element 219221, thereby using the electric heating element 219221 to heat the heating block 2192, and then heating the cracking tube or the eluting tube, so that the lysate in the cracking tube or the eluting tube in the eluting tube The liquid is maintained at the desired temperature.
  • each heating block 2192 has a second installation hole 21923, and a temperature protection switch 219231 is disposed in the second installation hole 21923.
  • the electric heating element 219221 is electrically connected to the external power supply, so that the external power supply provides electric energy for the electric heating element 219221, so that the heating element heats the heating block 2192, and the heating block 2192 transfers the heat to the lysate in the cracking tube or the eluent in the eluting tube. dehydration.
  • the temperature protection switch 219231 is electrically connected to the circuit between the electric heating element 219221 and the external power supply.
  • the temperature protection switch 219231 disconnects the electrical connection between the electric heating element 219221 and the external power supply, so that the electric heating element 219221 stops heating to prevent the temperature of the heating block 2192 from being too high.
  • each heating block 2192 also has a third installation hole 21924, and a temperature sensor 219241 is arranged in the third installation hole 21924, which is used to detect the temperature of the heating block 2192 in real time, so as to monitor the temperature of the heating block 2192. Monitoring is conducive to precise temperature control. Further, the temperature sensor 219241 is electrically connected to the electric heating element 219221.
  • the electric heating element 219221 When the temperature sensor 219241 detects that the temperature of the heating block 2192 is higher than the required temperature or temperature range, the electric heating element 219221 is controlled to reduce the heating power; when the temperature sensor 219241 detects When the temperature of the heating block 2192 is lower than the required temperature or temperature range, the electric heating element 219221 is controlled to increase the heating power, so as to ensure that the temperature of the heating block 2192 is maintained at the required temperature or temperature range to achieve precise temperature control.
  • the bearing seat 2191 is movably connected to the base 2181 along the second direction.
  • the bearing assembly 219 also includes a fourth drive assembly 2193 disposed on the base 2181, the fourth drive assembly 2193 is drivingly connected with the bearing base 2191, so as to drive the bearing base 2191 to move along the second direction to the position between the first magnetic bar assembly 217 and the The storage position below the first magnetic rod cover assembly 216 .
  • the fourth driving assembly 2193 includes a fourth screw 21931 , a fourth driving member 21932 and a fourth screw nut 21933 .
  • the fourth screw rod 21931 is rotatably connected to the base 2181 around its own axis, and the axial direction of the fourth screw rod 21931 is parallel to the second direction.
  • the fourth driving member 21932 is disposed on the base 2181 and is in transmission connection with the fourth screw rod 21931 to drive the fourth screw rod 21931 to rotate around its own axis.
  • the fourth screw nut 21933 is threadedly connected to the fourth screw rod 21931, so that when the fourth screw rod 21931 rotates, the fourth screw nut 21933 can be driven to move along the axial direction of the fourth screw rod 21931 (ie, the second direction).
  • the fourth screw nut 21933 is fixedly connected to the bearing base 2191 , so that the bearing base 2191 can move along the second direction along with the fourth screw nut 21933 .
  • the fourth driving member 21932 may be a motor.
  • the base 2181 is provided with a fourth sliding block 21811
  • the bearing base 2191 is provided with a third sliding rail 21911 extending lengthwise along the second direction.
  • the third sliding rail 21911 is connected to the fourth sliding block 21811 Sliding fit, so that the third sliding rail 21911 and the fourth sliding block 21811 are used to guide the movement of the bearing seat 2191 relative to the base 2181 along the second direction.
  • the nucleic acid extraction mechanism 210 also includes a housing assembly (not shown), which includes a housing 2110 arranged on a base 2181, a driving device, a first magnetic Both the rod cover assembly 216 and the first magnetic rod assembly 217 are located in the housing 2110 .
  • the housing 2110 has an inlet and outlet 2120 for the bearing seat 2191 to go in and out along the second direction.
  • the shell assembly also includes a hatch 2130 rotatably arranged on the base 2181, the hatch 2130 can be rotated relative to the base 2181 to a closed position for closing the entrance and exit 2120 and an open position for opening the entrance and exit 2120 (see FIG. 9 and FIG. 10).
  • the carrying seat 2191 moves outward from the position of entering the warehouse along the second direction through the inlet and outlet 2120 , the carrying seat 2191 pushes against the door 2130 to rotate from the closed position to the open position.
  • the housing assembly also includes a rotating shaft (not shown) and a second elastic member (not shown), one end of the hatch 2130 is rotatably connected to the base 2181 through the rotating shaft, and the second elastic member is arranged on the rotating shaft. And abutting against the door 2130 and the base 2181 , the second elastic member is used to provide an elastic force that makes the door 2130 turn from the open position to the closed position.
  • the second elastic member may be a torsion spring.
  • other types of springs can also be used, as long as the elastic force for driving the hatch door 2130 to rotate from the open position to the closed position can be provided under the condition that the hatch door 2130 is not subjected to external force, there is no limitation here.
  • the bearing seat 2191 is provided with a roller 21912 for abutting against the hatch door 2130, so that the roller 21912 abuts against the hatch door 2130, and the sliding friction is converted into rolling friction, so that the bearing seat 2191 can push the cabin more smoothly Door 2130 pivots to an open position.
  • the second embodiment of the present application also provides a nucleic acid extraction mechanism 230 , which includes an extraction vibration assembly 231 and a second kit carrying mechanism 232 .
  • the second kit carrying mechanism 232 is used to carry the second kit b.
  • the extracting vibration assembly 231 is used to insert or exit the second magnetic bar set 2321 into or withdraw from the second reagent box b, insert or withdraw the second magnetic bar 2341 from the second magnetic bar set 2321, and laterally transfer the second magnetic bar set 2321 and the second magnetic bar set 2321.
  • Two magnetic rods 2341 to complete steps such as nucleic acid lysing, mixing, transferring, and washing.
  • the extraction vibration assembly 231 includes a mounting plate 2310, a first mounting base 2320, a first driving assembly 212 (not shown), a second mounting base 2340 and a second mounting base 2340.
  • Drive assembly 214 (not shown).
  • the mounting plate 2310 has a first side 23101 and a second side 23102 opposite in a first direction.
  • the first installation seat 2320 is used for installing the second magnetic rod cover 2321 and is movably connected to the installation plate 2310 along the second direction.
  • the second direction is perpendicular to the first direction.
  • the first driving assembly 212 includes a first driving unit 2331 and a first transmission structure 2332 both disposed on the mounting plate 2310 .
  • the first driving unit 2331 is connected to the first mounting base 2320 through the first transmission structure 2332 to drive the first mounting base 2320 to move relative to the mounting plate 2310 along the second direction, thereby driving the second magnetic bar on the first mounting base 2320
  • the sleeve 2321 is inserted into or withdrawn from the second reagent box b, and reciprocates rapidly along the second direction.
  • the second mounting base 2340 is used for mounting the second magnetic bar 2341 and is movably connected to the mounting plate 2310 along the second direction.
  • the second mounting base 2340 and the above-mentioned first mounting base 2320 are arranged along the second direction, so that the second magnetic rod 2341 can be inserted into or withdrawn from the second magnetic rod sleeve 2321 when the second mounting base 2340 moves along the second direction.
  • the second driving assembly 214 includes a second driving unit 2354 and a second transmission structure 2350 both disposed on the mounting plate 2310 .
  • the second drive unit 2354 is connected to the second mounting base 2340 through the second transmission structure 2350 to drive the second mounting base 2340 to move relative to the mounting plate 2310 in the second direction, thereby driving the second magnetic bar on the second mounting base 2340 2341 is inserted into or withdrawn from the second magnetic rod sleeve 2321 .
  • the first transmission structure 2332 is located on the first side 23101 of the installation board 2310
  • the second transmission structure 2350 is located on the second side 23102 of the installation board 2310 . That is to say, the space of the first side 23101 and the second side 23102 of the mounting plate 2310 is used to set the first transmission structure 2332 and the second transmission structure 2350, so as to avoid occupying a direction parallel to the plane where the mounting plate 2310 is located (that is, the second direction and the space on the third direction below), which is conducive to improving space utilization and reducing the required space.
  • the first transmission structure 2332 and the second transmission structure 2350 are arranged on both sides of the mounting plate 2310 respectively, which avoids mutual influence, simplifies the structure, and facilitates later maintenance.
  • both the first mounting seat 2320 and the second mounting seat 2340 are located at one end of the mounting plate 2310 in the third direction.
  • the third direction is perpendicular to the first direction and the second direction.
  • the first transmission structure 2332 and the second transmission structure 2350 are respectively arranged on the first side 23101 and the second side 23102 of the installation plate 2310 in the first direction
  • the first installation seat 2320 and the second installation seat 2340 are arranged At one end of the mounting plate 2310 in the third direction, the layout is more reasonable, avoiding the space occupied by the first transmission structure 2332 and the second transmission structure 2350 in the third direction, which is beneficial to reducing the space required in the third direction.
  • the first direction, the second direction and the third direction are perpendicular to each other.
  • the first direction is the X direction in FIG. 18
  • the second direction is the Y direction in FIG. 18
  • the third direction is the Z direction in FIG. 18 .
  • the first driving unit 2331 is installed on the second side 23102 of the mounting plate 2310, and the output shaft of the first driving unit 2331 passes through the second side 23102 to the first side 23101, and is connected with the first transmission structure 2332 Drive connection.
  • the power is output from the output shaft of the first driving unit 2331 to the first transmission structure 2332, and the first transmission structure 2332 transmits the power to the first mounting base 2320, thereby driving the first mounting base 2320 to move in the second direction.
  • the second driving unit 2354 is installed on the second side 23102 of the mounting plate 2310, and the output shaft of the second driving unit 2354 passes through from the first side 23101 to the second side 23102, and is connected with the second transmission structure 2350 Drive connection.
  • the power is output from the output shaft of the second driving unit 2354 to the second transmission structure 2350, and the second transmission structure 2350 transmits the power to the second mounting base 2340, thereby driving the second mounting base 2340 to move along the second direction.
  • the first transmission structure 2332 includes a first driving pulley 23321 , a first driven pulley 23322 and a first synchronous belt 23323 .
  • the first driving pulley 23321 is installed on the output shaft of the first driving unit 2331 so that the first driving unit 2331 can drive the first driving pulley 23321 to rotate.
  • the first driven pulley 23322 is rotatably connected to the first side 23101 of the mounting plate 2310, and is spaced apart from the first driving pulley 23321 along the second direction, and the first timing belt 23323 is sheathed on the first driving pulley 23321 and the first driven pulley 23322, and is fixedly connected with the first installation seat 2320.
  • the first driving unit 2331 drives the first driving pulley 23321 to rotate, the first synchronous belt 23323 moves forward sequentially between the first driving pulley 23321 and the first driven pulley 23322, thereby driving the first mounting seat 2320 Move in the second direction.
  • the first driving unit 2331 may adopt a stepping motor.
  • the second transmission structure 2350 includes a second driving pulley 2351 , a second driven pulley 2352 and a second synchronous belt 2353 .
  • the second driving pulley 2351 is installed on the output shaft of the second driving unit 2354 so that the second driving unit 2354 can drive the second driving pulley 2351 to rotate.
  • the second driven pulley 2352 is rotatably connected to the second side 23102 of the mounting plate 2310, and is spaced apart from the second driving pulley 2351 along the second direction, and the second timing belt 2353 is sheathed on the second driving pulley 2351 between the second driven pulley 2352 and fixedly connected with the second installation base 2340 .
  • the second driving unit 2354 drives the second driving pulley 2351 to rotate, the second synchronous belt 2353 moves forward sequentially between the second driving pulley 2351 and the second driven pulley 2352, thereby driving the second mounting seat 2340 Move in the second direction.
  • the second driving unit 2354 may adopt a stepping motor.
  • both the first transmission structure 2332 and the second transmission structure 2350 are driven by synchronous belts, which have the advantages of stable transmission, good buffering and vibration reduction capabilities, and low noise.
  • the extracting vibration assembly 231 further includes a first guide rail 2311 , a first sliding part 2313 , a second sliding part 2312 , a first transmission plate 2314 and a second transmission plate 2315 .
  • the first guide rail 2311 is fixedly connected to the installation board 2310 and extends lengthwise along the second direction. Both the first sliding member 2313 and the second sliding member 2312 are slidingly fitted on the first guide rail 2311 , so that the first sliding member 2313 and the second sliding member 2312 can slide along the first guide rail 2311 independently of each other.
  • the first mounting base 2320 is fixedly connected to the first sliding member 2313 so as to move along with the first sliding member 2313 along the first guide rail 2311 (ie, the second direction).
  • the second mounting base 2340 is fixedly connected to the second sliding member 2312 so as to move along with the second sliding member 2312 along the first guide rail 2311 (ie, the second direction).
  • the first transmission plate 2314 is fixedly connected to the first sliding member 2313, and is fixedly connected to the first synchronous belt 23323 of the first transmission structure 2332, so that the first sliding member 2313 and the first mounting seat 2320 follow the first synchronous belt 23323 along the Move in the second direction.
  • the second transmission plate 2315 is fixedly connected to the second sliding member 2312, and is fixedly connected to the second synchronous belt 2353 of the second transmission structure 2350, so that the second sliding member 2312 and the second mounting seat 2340 follow the second synchronous belt 2353 along the Move in the second direction.
  • a first guide rail 2311 is used to simultaneously guide the movement of the first sliding member 2313 and the second sliding member 2312, realizing the movement of the first mounting base 2320 and the second mounting base 2340 along the second direction, simplifying the structure , further reducing the required space.
  • the extraction oscillation assembly 231 further includes a support base 2360 and a third drive assembly 2182 (not shown), the mounting plate 2310 is movably connected to the support base 2360 along the first direction, so that the first mounting base 2320 and the second mounting seat 2340 can follow the mounting plate 2310 to move along the first direction, so as to realize the transfer of the second magnetic rod sleeve 2321 and the second magnetic rod 2341 along the first direction.
  • the third driving assembly 2182 is mounted on the support base 2360 and is in transmission connection with the mounting plate 2310 to drive the mounting plate 2310 to move along the first direction.
  • the first drive assembly 212 is used to drive the first mount 2320 and the second magnetic bar cover 2321 on the first mount 2320 to move in the second direction, so that the second magnetic bar cover 2321 is inserted into or withdrawn from the second reagent box b , it can also realize that the second magnetic rod cover 2321 moves back and forth rapidly along the second direction to mix the sample solution in the second reagent box b.
  • the second mounting base 2340 and the second magnetic rod 2341 on the second mounting base 2340 are driven by the second driving assembly 214 to move along the second direction, so that the second magnetic rod 2341 is inserted into or withdrawn from the second magnetic rod sleeve 2321 .
  • the third driving assembly 2182 is used to drive the second magnetic rod set 2321 and the second magnetic rod 2341 to move together in the first direction, so as to realize the transfer of nucleic acid.
  • the extracting vibration assembly 231 further includes a base plate 2316 to which the mounting plate 2310 is fixedly connected.
  • the base plate 2316 is movably connected to the support base 2360 along the first direction, and is in transmission connection with the third drive assembly 2182, so that the third drive assembly 2182 can drive the base plate 2316 and the installation plate 2310 to move relative to the support base 2360 along the first direction. , and then drive the second magnetic rod sleeve 2321 and the second magnetic rod 2341 to move together along the first direction.
  • the extracting vibration assembly 231 further includes a second guide rail 2361 and a third sliding member 2362 , the second guide rail 2361 is fixedly connected to the support seat 2360 and extends lengthwise along the first direction.
  • the third sliding part 2362 is fixedly connected to the bottom plate 2316 and is slidingly matched with the second guide rail 2361 , so that the movement of the bottom plate 2316 relative to the support base 2360 along the first direction is guided by the second guide rail 2361 and the third sliding part 2362 .
  • the third drive assembly 2182 includes a third drive unit (not shown) and a third transmission structure (not shown), the third transmission structure includes a third driving pulley 2371, a third driven pulley 2372 and the third synchronous belt 2373.
  • the third driving unit is installed on the support base 2360, and the third driving pulley 2371 is installed on the output shaft of the third driving unit.
  • the third driven pulley 2372 is rotatably connected to the support base 2360 and is spaced apart from the third driving pulley 2371 along the first direction.
  • the third synchronous belt 2373 is sheathed between the third driving pulley 2371 and the third driven pulley 2372 , and is fixedly connected to the bottom plate 2316 .
  • the third driving unit can drive the third driving pulley 2372 to rotate, thereby driving the third synchronous belt 2373 to move forward sequentially between the third driving pulley 2371 and the third driven pulley 2372, thereby driving the bottom plate 2316 to move along the first Move in one direction.
  • the third drive unit may adopt a stepper motor.
  • the first installation base 2320 is located above the second reagent box b
  • the second installation base 2340 is located above the first installation base 2320 .
  • the second magnetic bar cover 2321 on the first mounting base 2320 is aligned with the first reaction hole 2541 of the second reagent box b
  • the second magnetic rod cover 2321 on the second mounting base 2340 is aligned.
  • the rod 2341 is aligned with the second magnetic rod sleeve 2321 on the first mount 2320 .
  • the first mounting base 2320 drives the second magnetic rod cover 2321 Descending until the second magnetic rod cover 2321 is inserted into the first reaction well 2541 of the second reagent box b (see FIG. 20 ).
  • the second mounting base 2340 drives the second magnetic rod 2341 down until it is inserted into the second magnetic rod cover 2321 (as shown in FIG. 21 ), so that the magnetic beads in the first reaction hole 2541 are adsorbed on the second magnetic rod cover. 2321 (that is, to extract the nucleic acid in the lysate).
  • the first mounting base 2320 and the second mounting base 2340 rise together until the second magnetic rod cover 2321 exits the first reaction hole 2541 of the second reagent box b (see FIG. 22 ).
  • the mounting plate 2310 drives the first mounting base 2320 and the second mounting base 2340 to move together in the first direction until the second magnetic rod cover 2321 and the second magnetic rod 2341 are aligned with the second reaction hole 2541 of the second kit b. Alignment (at this time, the second magnetic rod 2341 remains inserted into the second magnetic rod sleeve 2321).
  • the first mounting base 2320 and the second mounting base 2340 descend together until the second magnetic rod cover 2321 with magnetic beads is inserted into the second reaction hole 2541 (it can be understood that the second reaction hole 2541 contains with detergent).
  • the second mounting seat 2340 rises until the second magnetic rod 2341 exits the second magnetic rod sleeve 2321 .
  • the first mounting base 2320 is driven by the first driving unit 2331 to reciprocate rapidly up and down, so that the second magnetic rod cover 2321 vibrates in the up and down direction in the second reaction hole 2541, thereby realizing the transfer of nucleic acid to the washing chamber. Purified in liquid.
  • the magnetic beads adsorbed with nucleic acid are sequentially transferred between the reaction wells 2541 of the second kit b until reaching the last reaction well 2541 (such as the sixth reaction well 2541), so as to realize the detection of nucleic acid.
  • the second mounting seat 2340 rises until the second magnetic rod 2341 exits the second magnetic rod sleeve 2321 .
  • the first mounting base 2320 reciprocates up and down quickly, thereby driving the second magnetic bar cover 2321 to vibrate up and down, so that the nucleic acid extracted by the magnetic beads is incorporated into the eluent.
  • the first mounting base 2320 stops reciprocating up and down, and stays in the last reaction well 2541 .
  • the second mounting base 2340 descends until the second magnetic rod 2341 is inserted into the second magnetic rod cover 2321, and the magnetic beads are adsorbed on the second magnetic rod cover 2321 again (at this time, the nucleic acid has been incorporated into the eluent) .
  • the first mounting base 2320 and the second mounting base 2340 are lifted back to the initial position together, and the second reagent box b is taken away, and the second magnetic rod cover 2321 is replaced.
  • the third embodiment of the present application provides a nucleic acid extraction mechanism 250 , including an installation frame 2510 , a heating module 2520 and a first driving module 2530 .
  • the heating module 2520 is installed on the installation frame 2510, and is used for installing the second reagent box b, the second reagent box b has a reaction well 2541, and the reaction well 2541 contains reagents for extracting nucleic acid.
  • the heating module 2520 can heat the reagents in the reaction wells 2541 to increase the temperature of the reagents, thereby facilitating the cleavage and elution steps in the nucleic acid extraction process.
  • the first driving module 2530 is connected with the heating module 2520 to drive the heating module 2520 to reciprocate relative to the installation frame 2510 in the first direction, so as to facilitate the second reagent box b installed on the heating module 2520 to enter or exit the warehouse.
  • the nucleic acid extraction mechanism 250 also includes a magnetic rod cover carrier 2550 and a magnetic rod carrier 2560 that are all installed on the installation frame 2510.
  • the magnetic rod cover carrier 2550 is used to install the third magnetic rod cover assembly 2570, and the magnetic rod carrier 2560 is used.
  • the magnetic bar cover carrier 2550 can reciprocate in the second direction relative to the mounting frame 2510, so as to change the relative position of the third magnetic bar cover assembly 2570 mounted on the magnetic bar cover carrier 2550 in the second direction relative to the third magnetic bar. 2580 position.
  • the magnetic rod carrier 2560 can reciprocate in the second direction relative to the installation frame 2510, so as to change the position of the third magnetic rod 2580 mounted on the magnetic rod carrier 2560 relative to the third magnetic rod sleeve assembly 2570 in the second direction. Location.
  • the magnetic rod holder 2550 and the magnetic rod holder 2560 can move synchronously or asynchronously with respect to the installation frame 2510 in the second direction.
  • the first driving module 2530 drives the heating module 2520 to move in the first direction, so that the second kit b installed on the heating module 2520 enters the bin, that is, in the second direction, the second kit b It is located directly below the magnetic rod holder 2550 and the magnetic rod holder 2560 .
  • the magnetic rod cover carrier 2550 drives the third magnetic rod cover assembly 2570 to move in the second direction relative to the installation frame 2510, so that the third magnetic rod cover 2572 of the third magnetic rod cover assembly 2570 is plugged into the second kit b In the reaction well 2541.
  • the magnetic rod carrier 2560 drives the third magnetic rod 2580 to move in the second direction relative to the installation frame 2510, so that the third magnetic rod 2580 is plugged into the third magnetic rod sleeve 2572 of the third magnetic rod sleeve assembly 2570, so that The magnetic beads adsorbed with nucleic acid molecules are adsorbed on the outer surface of the third magnetic rod cover 2572 under the magnetic force of the third magnetic rod 2580 . Then, the magnetic rod cover carrier 2550 and the magnetic rod carrier 2560 move synchronously in the second direction relative to the second reagent box b, and at this time the third magnetic rod cover component 2570 is released from the reaction hole 2541 of the second reagent box b .
  • the magnetic rod holder 2550 and the magnetic rod holder 2560 move relative to the second reagent box b in the third direction, so that the third magnetic rod sleeve 2572 of the third magnetic rod sleeve assembly 2570 is plugged into the second reagent kit b In another reaction well 2541 of.
  • the magnetic rod carrier 2560 moves relative to the magnetic rod sleeve carrier 2550 in the second direction, and the third magnetic rod 2580 is released from the third magnetic rod sleeve 2572 of the third magnetic rod sleeve assembly 2570.
  • the magnetic beads will no longer Under the magnetic force applied by the third magnetic rod 2580, the magnetic beads are detached from the outer surface of the third magnetic rod sleeve 2572, so as to complete the transfer of the magnetic beads between the two reagents.
  • the first driving module 2530 drives the heating module 2520 to reversely move in the first direction, so that the second kit b mounted on the heating module 2520 is ejected from the warehouse, that is, in the second direction, the second kit b b is no longer located directly below the magnetic rod sleeve carrier 2550 and the magnetic rod carrier 2560 .
  • the movement of the third magnetic rod 2580 in the third magnetic rod cover assembly 2570 can also be controlled to oscillate and mix the reagents, which is beneficial to the steady progress of nucleic acid extraction.
  • the third magnetic bar set assembly 2570 is detachably connected to the magnetic bar set carrier 2550 in the first direction, and the first driving module 2530 drives the heating module 2520 in the first direction to drive the second kit b away from the magnetic bar set
  • the second reagent box b drives the third magnetic rod cover assembly 2570 inserted into the reaction hole 2541 to move to separate from the magnetic rod cover carrier 2550 . That is, when the second reagent box b is out of the warehouse, it can drive the third magnetic bar cover assembly 2570 inserted into the reaction hole 2541 to move so as to separate from the magnetic bar cover carrier 2550 .
  • the third magnetic rod cover assembly 2570 when the used third magnetic rod cover assembly 2570 needs to be removed from the magnetic rod cover carrier 2550, only the third magnetic rod of the third magnetic rod cover assembly 2570 needs to be
  • the cover 2572 is plugged into the reaction hole 2541 of the second reagent box b, the first driving module 2530 drives the heating module 2520 to move to drive the second reagent box b out of the warehouse, and the third magnetic rod cover assembly 2570 is placed in the second reagent box b Under the applied force, it is separated from the magnetic rod cover carrier 2550 along the first direction, so as to automatically remove the third magnetic rod cover assembly 2570 .
  • the nucleic acid extraction mechanism 250 provided in this embodiment has a simple structure and reduces costs.
  • the installation frame 2510 includes a frame body 2511 and a frame mounting plate 2512, the heating module 2520, the first driving module 2530 and the frame mounting plate 2512 are all mounted on the frame body 2511, and the magnetic rod is covered with a frame 2550 and the magnetic rod carrier 2560 are installed on the frame mounting plate 2512 .
  • the frame body 2511 includes a frame bottom plate 25111 and a mounting portion 25112 , the heating module 2520 , the first driving module 2530 and the mounting portion 25112 are installed on the frame bottom plate 25111 , and the frame mounting plate 2512 is connected to the mounting portion 25112 .
  • the first driving module 2530 includes a first driving motor 2531, a screw 2532 and a screw nut 2533, the screw 2532 is connected with the first driving motor 2531 and extends along the first direction, and the screw nut 2533 Connect with heating module 2520.
  • the first driving motor 2531 operates to drive the screw rod 2532 to rotate, and the rotation of the screw rod 2532 drives the screw nut 2533 to move in the first direction, thereby driving the heating module 2520 to move in the first direction.
  • the first driving motor 2531 drives the screw rod 2532 to rotate in different directions, and the heating module 2520 moves back and forth in the first direction in different directions, so that the second reagent box b installed on the heating module 2520 can enter and exit the compartment.
  • the first driving module 2530 is not limited to the above-mentioned way of driving the screw 2532 and the screw nut 2533 , and it can also be a belt drive or a gear drive.
  • One of the heating module 2520 and the frame bottom plate 25111 is provided with a first track 2590 extending along the first direction, and the other is provided with a first guide groove, and the heating module 2520 cooperates with the first guide groove through the first track 2590 .
  • the first track 2590 cooperates with the first guide groove to guide the heating module 2520 in the first direction.
  • the first track 2590 is set on the frame bottom plate 25111 , and the first guide groove is set on the heating module 2520 .
  • the first track 2590 is set on the heating module 2520
  • the first guide groove is set on the frame bottom plate 25111 .
  • the heating module 2520 is detachably connected to the installation frame 2510, and the heating module 2520 is controlled by an independent PCBN, so that the heating module 2520 only uses one wire to connect to the main control board, so that the heating module 2520 can be replaced when it is damaged.
  • the heating module 2520 is detachably connected to the screw nut 2533. After the heating module 2520 is detached from the screw nut 2533, the heating module 2520 can be separated from the installation frame 2510 in the first direction, so that the heating module 2520 can be separated from the The mounting frame 2510 is disassembled to facilitate the damaged replacement of the heating module 2520.
  • the heating module 2520 includes a heating body 2521 and at least one first heating strip 2522 and at least one second heating strip 2523 arranged on the heating body 2521.
  • the first heating strip 2522 and the second heating strip 2523 The third direction is arranged alternately and at intervals, and the first heating strips 2522 and the second heating strips 2523 both extend along the first direction.
  • the first heating strip 2522 is used for pyrolysis heating
  • the second heating strip 2523 is used for elution heating.
  • the heating module 2520 includes at least two first heating strips 2522 and at least two second heating strips 2523, and all the first heating strips 2522 and the second heating strips 2523 are sequentially spaced and staggered in the third direction.
  • the heating module 2520 is provided with at least two first heating strips 2522 for cracking, and at least two second heating strips 2523 for elution, and the heating module 2520 can crack and wash each time in the third direction. At least two samples are removed, which improves the efficiency of nucleic acid extraction. At the same time, since the heating strips for lysis and elution are arranged at intervals in the third direction, it is avoided that the temperature of elution is raised when the temperature of lysis is raised, and the mutual interference between lysis and elution is reduced.
  • Each of the first heating strip 2522 and the second heating strip 2523 is provided with a plurality of heating accommodation grooves 2524 in the first direction, and each heating accommodation groove 2524 is set corresponding to a reaction part on the second kit b, each Each reaction section is provided with a reaction hole 2541 .
  • the heating module 2520 can heat the reagent in the reaction hole 2541 of the reaction part inserted in the heating storage tank 2524, so as to Upward can lyse and elute multiple samples each time, further improving the efficiency of nucleic acid extraction.
  • the heating module 2520 includes two first heating strips 2522 and two second heating strips 2523. At this time, the heating module 2520 can lyse and elute two samples at a time in the third direction. Each of the first heating strips 2522 and each of the second heating strips 2523 is provided with 8 heating accommodation slots 2524 in the first direction, so that the heating module 2520 can lyse and elute 16 samples each time. It should be understood that, in some other embodiments, the number of the first heating strip 2522 and the second heating strip 2523 included in the heating module 2520 is not limited, and each first heating strip 2522 and each second heating strip The number of the heating accommodation grooves 2524 included in the bar 2523 is also not limited.
  • the heating body 2521 is provided with 12 installation positions in the third direction
  • one of the first heating strips 2522 is located at the second installation position
  • one second heating strip 2523 is located at the sixth installation position
  • the other one is at the first
  • the heating strip 2522 is set at the 8th installation position
  • another second heating strip 2523 is set at the 12th installation position. That is, the 2nd installation position and the 8th installation position are cracking positions, and the 6th installation position and the 12th installation position are elution positions.
  • the second kit b includes a 96-deep well plate.
  • the reaction parts corresponding to the 2nd, 6th, 8th and 12th rows of the 96 deep well plate are plugged into the first heating strip 2522 at the 2nd installation position and the second heating strip 2522 at the 6th installation position respectively.
  • the 96-deep well plate can achieve a throughput of 1-16 people.
  • the second kit b includes a second kit carrier 160 (see FIG. 28 ) and a single-serving reagent strip, and the second kit carrier 160 is installed on the heating module 2520, and the single-serving reagent The strips are carried on the second reagent box carrier 160 , and each reaction part of the reagent strip is inserted into each heating accommodation slot 2524 .
  • the second kit carrier 160 is provided with four placement grooves 25421 in the first direction, and at the same time, the second kit carrier 160 is provided with two placement grooves 25421 in the third direction, and each placement groove 25421 is used to place One single-serve test strip.
  • the second kit carrier 160 is capable of a throughput of 1-8 people.
  • the second kit carrier 160 is provided with a weight reducing hole 25422 to reduce the weight of the second kit carrier 160 .
  • the second kit b can also be configured in other ways, which is not limited here.
  • the nucleic acid extraction mechanism 250 further includes a second drive module 25120 installed on the installation frame 2510, and the second drive module 25120 is connected to the magnetic rod cover carrier 2550 to drive the magnetic rod cover carrier 2550 Reciprocating movement relative to frame mounting plate 2512 in a second direction.
  • the second driving module 25120 includes a second driving motor 251201 , a first driving wheel 251202 , a first driven wheel 251203 and a first transmission belt 251204 .
  • the first driving wheel 251202 is connected to the second driving motor 251201, the first driven wheel 251203 is connected to the frame mounting plate 2512, the first transmission belt 251204 is set outside the first driving wheel 251202 and the first driven wheel 251203, and the first transmission belt 251204 is connected to the first driving wheel 251204.
  • the magnetic bar sleeve carrier 2550 is fixedly connected.
  • the second driving motor 251201 acts, and the first driving wheel 251202 rotates to drive the first driven wheel 251203 to move through the first transmission belt 251204, and when the first transmission belt 251204 moves, it drives the magnetic bar sleeve carrier 2550 connected to it to move on the second drive belt 251204. direction of movement.
  • the nucleic acid extraction mechanism 250 also includes a third driving module 25130 installed on the mounting frame 2510, the third driving module 25130 is connected to the magnetic rod carrier 2560 to drive the magnetic rod carrier 2560 relative to the frame mounting plate 2512 in the second direction reciprocating motion.
  • the third driving module 25130 includes a third driving motor 251301 , a second driving wheel 251302 , a second driven wheel 251303 and a second transmission belt 251304 .
  • the second driving wheel 251302 is connected to the third drive motor 251301, the second driven wheel 251303 is connected to the frame mounting plate 2512, the second transmission belt 251304 is sleeved outside the second driving wheel 251302 and the second driven wheel 251303, and the second transmission belt 251304 is connected to the second driving wheel 251304.
  • the magnetic bar carrier 2560 is fixedly connected.
  • the third driving motor 251301 acts, and the second driving wheel 251302 rotates to drive the second driven wheel 251303 to move through the second transmission belt 251304, and when the second transmission belt 251304 moves, it drives the magnetic bar carrier 2560 connected to it in the second direction on sports.
  • second driving module 25120 and third driving module 25130 are not limited to the above-mentioned belt transmission, and they can also be driven by the screw 2532 and the screw nut 2533, or driven by gears.
  • the frame mounting plate 2512 includes a first frame mounting plate 25121 and a second frame mounting plate 25122 , both of which are perpendicular to the frame bottom plate 25111 .
  • the first frame mounting plate 25121 is connected to the mounting portion 25112, and the second frame mounting plate 25122 is connected to the first frame mounting plate 25121 and the two are perpendicular to each other.
  • Both the second driving module 25120 and the third driving module 25130 are installed on the first frame mounting plate 25121 , and the magnetic rod sleeve carrier 2550 and the magnetic rod carrier 2560 are both slidably connected with the second frame mounting plate 25122 in the second direction.
  • one of the second frame mounting plate 25122 and the magnetic bar sleeve holder 2550 is provided with a second track 25140 extending along the second direction, and the other is provided with a second guide groove, and the magnetic bar
  • the sleeve carrier 2550 cooperates with the second guide groove through the second rail 25140
  • the second rail 25140 cooperates with the second guide groove so as to guide the magnetic rod sleeve carrier 2550 in the second direction.
  • the second track 25140 is set on the second frame mounting plate 25122
  • the second guide groove is set on the magnetic rod sleeve carrier 2550 .
  • the second track 25140 is set on the magnetic bar sleeve carrier 2550
  • the second guide groove is set on the second frame mounting plate 25122 .
  • One of the second frame mounting plate 25122 and the magnetic rod carrier 2560 is provided with a third rail 25160 extending along the second direction, and the other is provided with a third guide groove, and the magnetic rod carrier 2560 passes through the third rail 25160 Cooperating with the third guide groove, the third track 25160 cooperates with the third guide groove to facilitate guiding the magnet bar carrier 2560 in the second direction.
  • the third track 25160 is set on the second frame mounting plate 25122
  • the third guide groove is set on the magnetic bar carrier 2560
  • the third track 25160 is arranged on the magnetic bar carrier 2560
  • the third guide groove is arranged on the second frame mounting plate 25122.
  • the second rail 25140 and the third rail 25160 are both arranged on the second frame mounting plate 25122, and the two are connected as one, the second guide groove is arranged on the magnetic bar sleeve carrier 2550, and the third guide groove The slot is set on the magnetic bar carrier 2560 .
  • the second guide groove and the third guide groove are both arranged on the second frame mounting plate 25122, and the two are connected, the second track 25140 is arranged on the magnetic bar sleeve carrier 2550, and the third track 25160 Set on the bar magnet carrier 2560 .
  • the magnetic rod cover carrier 2550 is set to be L-shaped, and one end of the L-shaped is slidably matched with the second frame mounting plate 25122, and the L-shaped The other end is used for installing the third magnetic rod sleeve assembly 2570 .
  • the magnetic bar carrier 2560 is set to be L-shaped, one end of the L-shaped is slidingly fitted with the second frame mounting plate 25122, and the other end of the L-shaped One end is used to install the third magnetic rod 2580.
  • the shapes of the magnetic rod holder 2550 and the magnetic rod holder 2560 there is no limitation on the shapes of the magnetic rod holder 2550 and the magnetic rod holder 2560, as long as they can be slidably connected to the second frame mounting plate 25122 and the third magnetic rod holder assembly 2570 can be installed. Or the setting method of the third magnetic bar 2580 can be used.
  • the magnetic bar cover carrier 2550 is provided with a sliding slot 2551 extending in the first direction, and the sliding slot 2551 is used for slidingly matching with the third magnetic bar cover assembly 2570 .
  • the third magnetic bar cover assembly 2570 is inserted into the reaction hole 2541 of the second kit b, and the first driving module 2530 drives the heating module 2520 drives the second reagent box b to move, and the second reagent box b applies force to the third magnetic rod cover assembly 2570, and the third magnetic rod cover assembly 2570 escapes from the chute 2551 from the end with the opening of the chute 2551 to automatically
  • the removal of the third magnetic rod cover assembly 2570 from the magnetic rod cover carrier 2550 is completed.
  • the magnetic rod cover carrier 2550 is provided with an insertion port 2552 communicating with the sliding slot 2551 to facilitate insertion of the third magnetic rod 2580 into the third magnetic rod cover 2572 from the insertion port 2552 .
  • the magnetic bar cover carrier 2550 includes at least two slide grooves 2551 arranged at intervals in the third direction, so that the magnetic bar cover carrier 2550 can simultaneously assemble at least two third magnetic bar cover assemblies 2570 in the third direction to improve nucleic acid extraction efficiency.
  • the magnetic rod sleeve carrier 2550 is provided with two sliding slots 2551 at intervals in the third direction, and the two sliding slots 2551 can be assembled with two third magnetic rod sleeve assemblies 2570, so as to realize two-serving flux in the third direction.
  • each third magnetic bar cover assembly 2570 includes an assembly part 2571 and a third magnetic bar cover 2572 disposed on the assembly part 2571 , and the assembly part 2571 is slidably connected with the magnetic bar cover carrier 2550 in the first direction.
  • Each third magnetic rod housing assembly 2570 has a plurality of third magnetic rod housings 2572 in the first direction to enable multi-serving throughput in the first direction.
  • each third magnetic rod housing assembly 2570 has 8 third magnetic rod housings 2572 in the first direction to achieve a throughput of 8 servings in the first direction.
  • the number of the third magnetic rods 2580 provided on the magnetic rod carrier 2560 in the third direction is equal to the number of the third magnetic rod cover assemblies 2570, and the number of the third magnetic rods 2580 provided in the first direction is equal to the third magnetic rods 2580.
  • the number of rod sets 2572 is equal, so that the third magnetic rods 2580 correspond to the third magnetic rod sets 2572 one by one.
  • the third magnetic rod 2580 adopts a magnetic flux of nearly 5000 Gauss, which can effectively adsorb nano-scale magnetic beads and achieve highly purified nucleic acid.
  • the nucleic acid extraction mechanism 250 also includes a fourth driving module 25180, the fourth driving module 25180 is arranged on the mounting part 25112, and is connected with the first frame mounting plate 25121 to drive the frame mounting plate 2512 relative to The frame body 2511 reciprocates in the third direction, thereby driving the magnetic rod cover carrier 2550 and the magnetic rod carrier 2560 to reciprocate in the third direction, and then the third magnetic rod cover 2572 and the third magnetic rod cover 2572 installed on the magnetic rod cover carrier 2550
  • the third magnetic rod 2580 installed on the magnetic rod carrier 2560 reciprocates in the third direction, so that the third magnetic rod cover 2572 and the third magnetic rod 2580 are located above each reaction well 2541 to facilitate nucleic acid extraction.
  • the fourth driving module 25180 includes a fourth driving motor 251801, a third driving wheel 251802, a third driven wheel 251803 and a third transmission belt 251804.
  • the motor 251801 is connected, the third transmission belt 251804 is sheathed outside the third driving wheel 251802 and the third driven wheel 251803, and the first frame mounting plate 25121 is connected to the third transmission belt 251804.
  • the fourth driving motor 251801 moves, the third driving wheel 251802 moves, the third driven wheel 251803 is driven to rotate through the third transmission belt 251804, and the third transmission belt 251804 drives the first frame mounting plate 25121 and the second frame mounting plate 25122 in the third direction synchronized movement.
  • the magnetic bar holder 2550 and the magnetic bar carrier 2560 mounted on the second frame mounting plate 25122 move together in the third direction.
  • the above-mentioned fourth driving module 25180 is not limited to the above-mentioned belt transmission, and it can also be driven by the screw rod 2532 and the screw nut 2533, or a gear drive.
  • the nucleic acid extraction mechanism 250 also includes a fan and a filter.
  • the fan generates suction, which can make the airflow flow from the front end of the nucleic acid extraction mechanism 250 to the rear end, that is, from one end to the other end where the heating module 2520 is installed, and the above-mentioned airflow flows to the outside through the filter, so that , reducing the damage to the human body caused by the aerosol suspended in the air during nucleic acid extraction.
  • the nucleic acid extraction mechanism 250 further includes an ultraviolet lamp 25190, and the ultraviolet lamp 25190 is arranged above the magnetic bar carrier 2560 in the second direction, so as to achieve the effect of disinfection.
  • the nucleic acid extraction mechanism 250 provided in the third embodiment above has the following beneficial effects:
  • the first driving module 2530 drives the heating module 2520 to move to drive the second reagent box b out of the warehouse, and the third magnetic rod cover assembly 2570 contacts the magnetic rod along the first direction under the force exerted by the second reagent kit b.
  • the sleeve carrier 2550 is separated to automatically remove the third magnetic rod sleeve assembly 2570 .
  • the heating module 2520 adopts independent PCBN control, which reduces the heating module 252024 wires to one connected to the main control board, which greatly simplifies the wiring.
  • the heating module 2520 is detachably connected to the installation frame 2510 to facilitate replacement of the heating module 2520 .
  • the heating module 2520 can be adapted to 96 deep-well plates and single-serve reagent strips at the same time, meeting the throughput of 1-16 servings.
  • the nucleic acid extraction mechanism 250 is small in size and light in weight.
  • the length, width, and height of the nucleic acid extraction mechanism 250 can be designed to be 190mm*410mm*385mm, and the weight can be designed to be 16Kg, which greatly saves costs.
  • the first pipetting mechanism 40 includes the fifth drive assembly, the first transfer base 42 (see FIG. 2 ) and the first pipette assembly, the fifth drive assembly is connected with the first mounting frame 10, and the first transfer base 42 is connected with the first transfer base 42.
  • the five driving components are connected, and the first pipetting component is connected with the first pipetting base.
  • the fifth driving assembly can drive the first transfer seat 42 to move in the first direction, the second direction and the third direction, and the first transfer seat 42 drives the first pipetting assembly to move in the first direction, the second direction and the third direction sports.
  • the first pipetting component is used to absorb the nucleic acid extracted by the nucleic acid extraction mechanism, and transfer it to the first kit a carried by the carrying mechanism 30 for amplification.
  • the fifth driving assembly includes a plurality of linear modules connected to drive the first transfer base 42 to drive the first pipetting assembly to move in the first direction, the second direction and the third direction.
  • the first linear module is connected to the first mounting frame 10
  • the second linear module is connected to the first linear module
  • the third linear module is connected to the second linear module
  • the first transfer seat 42 is connected to the third linear module.
  • the third linear module drives the first transfer seat 42 to drive the first pipetting assembly to reciprocate in the third direction
  • the second linear module drives the third linear module to drive the first transfer seat 42 and the first pipetting assembly
  • the reciprocating movement in the second direction the first linear module drives the second linear module to drive the third linear module, the first transfer base 42 and the first pipetting assembly to reciprocate in the first direction.
  • the first linear module, the second linear module and the third linear module can be synchronous belt linear modules or screw rod 2532 linear modules.
  • the fifth driving assembly can also adopt other driving structures, as long as it can drive the first transfer seat 42 to drive the first pipetting assembly in the first direction, the second direction and the third direction. Exercise is enough, and there is no limitation here.
  • the clamping mechanism 50 includes a sixth drive assembly, a clamping seat and a first jaw, the sixth drive assembly is connected to the first installation frame 10, the clamping seat is connected to the sixth drive assembly, and the first clamping jaw is connected to the clamping seat .
  • the sixth driving assembly can drive the clamping seat to drive the first jaw to move in the first direction, the second direction and the third direction.
  • the holding seat can drive the first jaw to hold the first kit a or release the first kit a.
  • the sixth drive assembly includes a plurality of linear modules connected to drive the clamping seat to drive the first jaw to move in the first direction, the second direction and the third direction.
  • the setting method of the sixth driving component can refer to the setting method of the fifth driving component.
  • the sixth drive assembly and the fifth drive assembly may share part of the linear module.
  • the holding seat can adopt an electric or pneumatic method to enable the first jaw to hold the first kit a or release the first kit a, and the specific method is not limited here.
  • the nucleic acid extraction device 200 also includes a sample loading mechanism 60, a clamping mechanism 70, a sample transfer mechanism 80, and a second pipetting mechanism 90 all installed on the first mounting frame 10. .
  • the sample loading mechanism 60 is used to store the sampling tube d.
  • one sampling tube d can be stored in the sample loading mechanism 60 .
  • several sampling tubes d can also be stored in the sample loading mechanism 60 at the same time, so as to facilitate subsequent simultaneous nucleic acid extraction from liquids in multiple sampling tubes d.
  • the clamping mechanism 70 has a clamping position for clamping or loosening the sampling tube d.
  • the sample transfer mechanism 80 is used to transfer the sampling tube d between the sample loading mechanism 60 and the clamping mechanism 70 , and the sample transfer mechanism 80 is also used to uncap or seal the sampling tube d clamped in the clamping mechanism 70 .
  • the second pipetting mechanism 90 is used to suck the sample in the sampling tube d clamped by the clamping mechanism 70 and transfer it to the nucleic acid extraction mechanism, so that the nucleic acid extraction mechanism can extract nucleic acid.
  • the sampling tube d containing the sample to be detected is placed on the sample loading mechanism 60 , and the sample transfer mechanism 80 moves above the sample loading mechanism 60 to clamp the sampling tube d. Then, the sample transfer mechanism 80 moves to the clamping mechanism 70, and places the clamped sampling tube d in the clamping position of the clamping mechanism 70, and the clamping mechanism 70 clamps the sampling tube d. Then, the sample transfer mechanism 80 unscrews the cap of the sampling tube d to complete uncapping, and the second pipetting mechanism 90 moves to the clamping mechanism 70 to suck the sample in the sampling tube d. The second pipetting mechanism 90 moves to the nucleic acid extraction mechanism, and releases the aspirated sample to the nucleic acid extraction mechanism.
  • the nucleic acid extraction mechanism extracts nucleic acid from the sample.
  • the sample transfer mechanism 80 tightens the cap on the sampling tube d, so as to prevent the residual sample in the sampling tube d from polluting other samples, or to facilitate the retention of the residual sample in the sampling tube d.
  • the clamping mechanism 70 releases the sampling tube d, and the sample transfer mechanism 80 transfers the sampling tube d to the sample loading mechanism 60 again. Cycle through the steps above. Until the samples in all the sampling tubes d on the sample loading mechanism 60 are transferred to the nucleic acid extraction mechanism, so that the nucleic acid extraction mechanism can perform nucleic acid extraction on each sample.
  • the nucleic acid extraction device 200 in the nucleic acid extraction and detection device 100 provided in the embodiment of the present invention can automatically realize the cap opening or capping through the sample transfer mechanism 80 .
  • the automation degree is high, the labor intensity of the staff is reduced, and the contamination of the sample by manual operation is reduced.
  • the first installation frame 10 is provided with a first waste area 110 (refer to FIG. 5 ), and after the clamping mechanism 70 releases the sampling tube d, the sample transfer mechanism 80 will suck the sampling tube d away from the sample. Discarded through the first discarding area 110.
  • the first discarding area 110 is a first discarding hole opened on the first installation frame 10 .
  • the nucleic acid extraction device 200 further includes a first waste storage box for receiving the sampling tube d discarded through the first waste hole.
  • the sample transfer mechanism 80 includes a seventh drive assembly and a cover-opening robot 82 (see FIG. 1 ), the seventh drive assembly is installed on the first installation frame 10, and the cover-opening robot 82 is connected to the seventh drive assembly.
  • the seven driving components are used to drive the cap opening manipulator 82 to move in the first direction, the second direction and the third direction.
  • the cap opening manipulator 82 is moved between the clamping positions of the sample loading mechanism 60 and the clamping mechanism 70 to realize the transfer of the sampling tube d.
  • the cover-opening manipulator 82 is used to hold the sampling tube d, and use the rotating motion to loosen or tighten the tube cover of the sampling tube d.
  • the seventh driving assembly includes a plurality of linear modules, which are connected to drive the cover-opening manipulator 82 to move in the first direction, the second direction and the third direction.
  • the arrangement manner of the seventh drive assembly can refer to the arrangement manner of the fifth drive assembly.
  • the seventh drive assembly may share part of the linear module with the fifth drive assembly or the sixth drive assembly.
  • the second pipetting mechanism 90 includes the eighth drive assembly, the second transfer base 92 (referring to Fig. 2 ) and the second pipette assembly, the eighth drive assembly is connected with the first mounting frame 10, and the second transfer base 92 is connected with the second transfer base 92.
  • the eight driving components are connected, and the second pipetting component is connected with the second transfer base 92 .
  • the eighth drive assembly can drive the second transfer seat 92 to move in the first direction, the second direction and the third direction, and the second transfer seat 92 drives the second pipetting assembly to move in the first direction, the second direction and the third direction sports.
  • the second pipetting component is used to suck the sample into the nucleic acid extraction mechanism.
  • the eighth driving assembly includes a plurality of linear modules connected to drive the second transfer base 92 to drive the second pipetting assembly to move in the first direction, the second direction and the third direction.
  • the setting manner of the eighth driving component can refer to the setting way of the fifth driving component.
  • the eighth drive assembly and the fifth drive assembly or the sixth drive assembly or the seventh drive assembly may share part of the linear module.
  • the nucleic acid extraction device 200 also includes a tip carrier 120 installed on the first installation frame 10 , and the tip carrier 120 is used to store the tip.
  • the first pipetting assembly of the first pipetting mechanism 40 can pick up the gun tip on the gun tip carrier 120, and the first pipetting mechanism 40 can absorb the nucleic acid extracted by the nucleic acid extraction mechanism through the gun tip, and release the nucleic acid to the first In kit a.
  • the first pipetting component can remove the tip.
  • the tip carrier 120 includes five, and the five tip carriers 120 are sequentially arranged at intervals in the first direction. It should be understood that, in some other embodiments, the number of gun tip carriers 120 is not limited.
  • the first installation frame 10 is provided with a second waste area 130 (see FIG. 5 ). After the first moving liquid mechanism releases the gun head, the gun head can be discarded through the second waste area 130 .
  • the second discarding area 130 is a second discarding hole opened on the first installation frame 10 .
  • the nucleic acid extraction device 200 further includes a second waste storage box for receiving pipette tips discarded through the second waste hole.
  • the eighth driving component of the second pipetting mechanism 90 can drive the second pipetting component to pass through the tip carrier 120 , the clamping position of the clamping mechanism 70 and the nucleic acid extraction mechanism.
  • the second pipetting component passes through the tip carrier 120
  • the second pipetting component can pick up the tips on the tip carrier 120 .
  • the second pipetting assembly moves to the clamping position of the clamping mechanism 70
  • the second pipetting assembly can absorb the sample in the sampling tube d clamped at the clamping position through the gun head.
  • the second pipetting assembly moves to the nucleic acid extraction mechanism, the second pipetting assembly can release the sample in the tip into the nucleic acid extraction mechanism.
  • the nucleic acid extraction device 200 further includes a second reagent carrier 140 installed on the first installation frame 10 , the second reagent carrier 140 is used to store a second reagent tube, and the second reagent tube stores a reagent for extracting nucleic acid.
  • the second pipetting assembly can pick up the tips on the tip carrier 120 .
  • the second pipetting component can absorb the reagent for nucleic acid extraction stored in the second reagent tube on the second reagent carrier 140 through the gun tip.
  • the second pipetting component moves to the nucleic acid extraction mechanism, the second pipetting component can release the reagent for nucleic acid extraction in the pipette tip into the nucleic acid extraction mechanism for nucleic acid extraction.
  • there is one second reagent carrier 140 and a plurality of second reagent tubes can be stored on the second reagent carrier 140 , and different reagents for extracting nucleic acid are stored in the plurality of second reagent tubes.
  • three second reagent tubes are stored on the second reagent carrier 140, the lysate is stored in one second reagent tube, the washing solution is stored in one second reagent tube, and the remaining one is stored in the second reagent tube. eluent.
  • the number of second reagent racks is not limited, and the number of second reagent tubes that can be stored on the second reagent carrier 140 is not limited.
  • the sample carrier is arranged at one end of the first installation frame 10, and the clamping mechanism 70, the nucleic acid extraction mechanism, the tip carrier 120 and the second reagent carrier 140 are arranged at the end of the first installation frame 10.
  • the carrying mechanism 30 is disposed at the other end of the first installation frame 10 .
  • the clamping mechanism 70, the nucleic acid extraction mechanism and the carrying mechanism 30 are arranged at one end of the first mounting frame 10, and the tip carrier 120 and the second reagent carrier 140 are arranged at the other end of the first mounting frame 10 .
  • the nucleic acid extraction mechanism is located between the clamping mechanism 70 and the carrying mechanism 30 . Such an arrangement facilitates pipetting by the first pipetting mechanism 40 and the second pipetting mechanism 90 , and improves work efficiency.
  • the nucleic acid extraction device 200 further includes a first kit carrier 150 installed on the first installation rack 10 for storing the first kit a.
  • the first jaw of the clamping mechanism 50 can clamp the first reagent box a stored on the first reagent box carrier 150.
  • the first clamp of the clamping mechanism 50 The claws can release the first reagent cartridge a onto the carrying mechanism 30 .
  • the nucleic acid extraction device 200 also includes a second reagent box carrier 160 mounted on the first installation rack 10 for storing the second reagent box b.
  • the first jaw of the clamping mechanism 50 can clamp the second reagent box b stored on the second reagent box carrier 160.
  • the clamping mechanism 50 moves to the nucleic acid extraction mechanism, the first jaw of the clamping mechanism 50
  • the second kit b can be released onto the nucleic acid extraction mechanism.
  • the nucleic acid extraction device 200 also includes a first reagent carrier 170 installed on the first installation frame 10, the first reagent carrier 170 is used to store the first reagent tube, the first reagent tube is used to store the nucleic acid reaction reagent, and the nucleic acid reaction reagent can An amplification reaction is performed with the extracted nucleic acid.
  • the first pipetting component of the first pipetting mechanism 40 can pick up the tips on the tip carrier 120 .
  • the first pipetting component moves to the first reagent carrier 170
  • the first pipetting component can absorb the nucleic acid reaction reagent stored in the first reagent tube on the first reagent carrier 170 through the tip.
  • the first pipetting assembly moves to the carrying mechanism 30, the first pipetting assembly can release the nucleic acid reaction reagent in the gun tip to the first kit a on the carrying mechanism 30, so as to be used for carrying out with the extracted nucleic acid.
  • Amplification reaction is performed with the extracted nucleic acid.
  • the nucleic acid extraction device 200 further includes a sealing film carrier 180 and a sealing film mechanism 190 mounted on the first installation frame 10 .
  • the sealing film carrier 180 is used to store the sealing film
  • the sealing film mechanism 190 is used to absorb the sealing film stored on the sealing film carrier 180, and is used to seal the sealing film on the first kit a carried on the carrier mechanism 30 .
  • the film carrier 180 includes two.
  • the sealing film mechanism 190 After nucleic acid and nucleic acid reaction reagents are added in the first kit a, when the sealing film mechanism 190 approaches the sealing film carrier 180, the sealing film mechanism 190 absorbs the sealing film stored on the sealing film carrier 180, and relative to the first installation The frame 10 moves to the carrying mechanism 30 .
  • the sealing mechanism 190 can seal the sealing film on the first kit a on the carrying mechanism 30, so as to detect the nucleic acid after the amplification reaction.
  • the sealing mechanism 190 is integrated with the clamping mechanism 50 . It should be understood that, in some other embodiments, the sealing mechanism 190 and the clamping mechanism 50 may also be separately and independently provided.
  • sealing mechanism 190 and the clamping mechanism 50 are integrated, their respective functions will not be affected.
  • the nucleic acid extraction device 200 also includes a sample holder 240 (see FIG. 2 ) installed on the first mounting frame 10, and the clamping mechanism 50 is used to clamp the first kit carried on the carrying mechanism 30. a. Retain a sample on the sample retention carrier 240, so as to facilitate the observation of the reserved sample.
  • the first reagent box carrier 150, the second reagent box carrier 160, the sealing membrane carrier 180, and the first reagent carrier 170 are all located on the same side of the first mounting frame 10 as the carrying mechanism 30. end. It is conceivable that, in some other embodiments, the positions of the above-mentioned carriers on the first installation frame 10 are not specifically limited.
  • the transfer mechanism 304 includes a ninth drive assembly, a transfer base and a second jaw, the ninth drive assembly is connected to the second mounting frame 301, the transfer base is connected to the ninth drive assembly, and the second jaw is connected to the transfer base connect.
  • the ninth driving assembly can drive the transfer base to drive the second jaw to move in the first direction, the second direction and the third direction.
  • the transfer seat can drive the second jaw to clamp the first reagent box a on the transfer plate 3021 to the nucleic acid detection mechanism 303 .
  • the ninth driving assembly includes a plurality of linear modules connected to drive the transfer base to move in the first direction, the second direction and the third direction.
  • the fourth linear module is connected to the second mounting frame 301 , the fifth linear module is connected to the fourth linear module, the sixth linear module is connected to the fifth linear module, and the transfer seat is connected to the sixth linear module.
  • the sixth linear module drives the transfer seat to drive the second jaw to reciprocate in the third direction; the fifth linear module drives the sixth linear module to drive the transfer seat and the second jaw to reciprocate in the second direction;
  • the module drives the fifth linear module to drive the sixth linear module, the transfer seat and the second jaw to reciprocate in the first direction.
  • the fourth linear module, the fifth linear module and the sixth linear module can be synchronous belt linear modules or screw rod 2532 linear modules.
  • the ninth drive assembly can also adopt other drive structures, as long as it can drive the transfer seat to drive the second jaw to move in the first direction, the second direction and the third direction. This is not limited.
  • the transfer base can use an electric or pneumatic method to make the second jaw clamp the first kit a or release the first kit a, and the specific method is not limited here.
  • the nucleic acid detection device 300 has a plurality of nucleic acid detection mechanisms 303 to facilitate simultaneous detection of nucleic acids in multiple first kits a and improve detection efficiency.
  • the nucleic acid detection device 300 includes three nucleic acid detection mechanisms 303 .
  • the number of nucleic acid detection mechanisms 303 included in the nucleic acid detection device 300 is not limited.
  • An embodiment of the present invention also provides a nucleic acid extraction and detection method using the nucleic acid extraction and detection device 100 described in any of the above embodiments, comprising steps:
  • the nucleic acid detection device 300 is located at one end of the nucleic acid extraction device 200 provided with the carrying mechanism 30 in the first direction.
  • the transfer plate 3021 protrudes into the first installation frame 10 in the first direction.
  • the nucleic acid extraction mechanism performs nucleic acid extraction
  • the first pipetting mechanism 40 sucks the nucleic acid extracted by the nucleic acid extraction mechanism to the first kit a carried by the carrying mechanism 30 for amplification.
  • the clamping mechanism 50 clamps the first reagent box a carried on the carrying mechanism 30 to the transfer plate 3021 .
  • the transfer plate 3021 of the transfer mechanism 302 is switched from the second position to the first position.
  • the transfer mechanism 304 transfers the first kit a carried on the transfer plate 3021 to the nucleic acid detection mechanism 303, and the nucleic acid detection mechanism 303 performs nucleic acid detection.
  • steps S10 and S20 it also includes:
  • the sample transfer mechanism 80 transfers the sampling tube d on the sample carrier to the clamping position of the clamping mechanism 70 .
  • the clamping mechanism 70 clamps the sampling tube d at the clamping position.
  • the second pipetting mechanism 90 moves to the clamping position, and sucks the sample in the sampling tube d located at the clamping position.
  • the second pipetting mechanism 90 moves to the nucleic acid extraction mechanism to release the aspirated sample into the nucleic acid extraction mechanism.
  • the sample transfer mechanism 80 re-tightens the cap on the sampling tube d at the clamping position, and retransfers the sampling tube d at the clamping position to the sample carrier or removes the sampling tube d from the first waste area 110 throw away.
  • steps S60 to S110 can be executed cyclically in sequence until the samples in each sampling tube d on the sample carrier are transferred to the nucleic acid extraction mechanism. And, when the number of samples in one of the nucleic acid extraction institutions is sufficient, the nucleic acid extraction institution begins to extract nucleic acids.
  • S10 and S60 it also includes:
  • the second pipetting mechanism 90 moves to the second reagent carrier 140, and sucks the reagent for nucleic acid extraction in the second reagent tube into the nucleic acid extraction mechanism.
  • step S110 and step S20 it also includes:
  • the first pipetting mechanism 40 moves to the first reagent carrier 170, and sucks the nucleic acid reaction reagent in the first reagent tube into the first reagent box a on the carrying mechanism 30.
  • step S20 and step S30 it also includes:
  • the sealing film mechanism 190 moves to the sealing film carrier 180, and absorbs the sealing film stored on the sealing film carrier 180.
  • the film sealing mechanism 190 moves to the carrying mechanism 30, and seals the film on the first reagent box a carried on the carrying mechanism 30. .

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Abstract

L'invention concerne un dispositif d'extraction et de détection d'acide nucléique et un procédé d'extraction et de détection d'acide nucléique. Le dispositif d'extraction et de détection d'acide nucléique (100) comprend : un premier cadre de montage (10) ; et un mécanisme d'extraction d'acide nucléique, un mécanisme de support de charge (30), un premier mécanisme de mouvement de liquide (40), et un mécanisme d'engagement (50) tous montés sur le premier cadre de montage (10) ; le mécanisme de support de charge (30) est utilisé pour porter un premier kit (a) ; le premier mécanisme de déplacement de liquide (40) est utilisé pour aspirer un acide nucléique extrait par le mécanisme d'extraction d'acide nucléique et le déplacer vers le premier kit (a) porté par le mécanisme de support de charge (30) pour l'amplification ; un second cadre de montage (301) ; et un mécanisme de transfert de charge (302), un mécanisme de détection d'acide nucléique (303), et un mécanisme de transfert (304) tous montés sur le second cadre de montage (301) ; le mécanisme de transfert de charge (302) comprend une plaque de transfert de charge (3021) utilisée pour supporter le premier kit (a) ; et lorsque la plaque de transfert de charge (3021) se situe à une première position, le mécanisme de transfert (304) est utilisé pour transférer le premier kit (a) sur la plaque de transfert de charge (3021) vers le mécanisme de détection d'acide nucléique (303) ; où, lorsqu'un appareil d'extraction d'acide nucléique (200) et un appareil de détection d'acide nucléique (300) sont connectés l'un à l'autre, la plaque de transfert de charge (3021), lorsqu'elle est à une seconde position, peut s'étendre à l'intérieur du premier cadre de montage (10), et le mécanisme d'engagement (50) peut engager le premier kit (a) porté par le mécanisme de support de charge (30) et le déplacer sur la plaque de transfert de charge (3021).
PCT/CN2022/119336 2021-09-17 2022-09-16 Dispositif d'extraction et de détection d'acide nucléique et procédé d'extraction et de détection d'acide nucléique WO2023041042A1 (fr)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
CN202122258839.3U CN216155875U (zh) 2021-09-17 2021-09-17 提取震荡机构及核酸提取仪
CN202122258839.3 2021-09-17
CN202111678985.XA CN114317230B (zh) 2021-12-31 2021-12-31 核酸提取检测设备及核酸提取检测方法
CN202123429987.3 2021-12-31
CN202123429987.3U CN216738297U (zh) 2021-12-31 2021-12-31 一种核酸提取装置
CN202111678985.X 2021-12-31
CN202221043103.2U CN217265654U (zh) 2022-05-05 2022-05-05 核酸提取装置
CN202221043103.2 2022-05-05

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Publication number Priority date Publication date Assignee Title
CN116269524A (zh) * 2023-05-24 2023-06-23 深圳市华瑞康生物医疗器械科技有限公司 一种便携式鼻咽拭子采样装置
CN116754728A (zh) * 2023-06-05 2023-09-15 广州工程技术职业学院 食品检测装置及其检测方法
CN117126727A (zh) * 2023-09-18 2023-11-28 合肥达徽基因科技有限公司 一种全自动提取扩增杂交一体化工作站

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