WO2023033414A1 - Liposomal cosmetic composition with excellent capture performance of effective component - Google Patents

Abstract

The present invention relates to a composition comprising liposomes containing: a biologically active material; a carbonaceous material including at least one selected from the group consisting of fullerene and fullerol; a surfactant; and a lipid, and provides a liposome-containing composition wherein the liposomes are superb in capture performance of effective components, stability, and dermal penetration, whereby the effective components bring about a remarkable improvement in skin whitening, wrinkle reduction, etc.

Description

Liposomal cosmetic composition with excellent trapping ability of active ingredients

It relates to a liposome cosmetic composition with excellent trapping ability of active ingredients.

The skin is composed of the epidermis, dermis, and subcutaneous fat, among which the epidermis is composed of a basal layer, a spinous layer, a granular layer, and a stratum corneum. The stratum corneum has a structure in which keratinocytes are stacked in multiple layers, and a lipid component exists in the empty space between keratinocytes.

At this time, it is reported that the transdermal absorption route is more effective when penetrating the lipid component located between the keratinocytes of the skin rather than directly penetrating the keratinocytes of the skin. There is increasing interest in skin absorption delivery systems for ingredients.

In particular, liposomes improve the stability of cosmetic compositions by collecting and containing ingredients that are poorly soluble or difficult to be stably supported in cosmetic composition formulations while increasing the skin penetration rate of active ingredients. development is becoming more active.

For example, Korean Patent Registration No. 10-0758960 discloses a cosmetic composition containing a nanoliposome-stabilized persimmon fruit extract as a skin improvement component.

On the other hand, as disclosed in the registered patent, development of cosmetic compositions using liposomes that capture active ingredients that exhibit specific effects is active, but most of them only capture a single ingredient that exhibits a single function, such as whitening, wrinkle improvement, Development of a liposome cosmetic composition containing a plurality of active ingredients to simultaneously exhibit various functions such as moisturizing is lacking.

In addition, most cosmetic compositions, although there are differences depending on the method of use, are left on average at room temperature for at least one month. In the case of liposome cosmetic compositions, stability is lower than that of general cosmetic compositions, and over time, liposomes There was a problem that the particle size became non-uniform and components trapped in the liposome were precipitated.

Accordingly, there is a demand for the development of a liposome cosmetic composition that includes a plurality of active ingredients to exhibit various functions at the same time, has excellent trapping power of active ingredients, and has excellent stability.

One aspect provides a cosmetic composition containing liposomes capable of implementing various functions including a plurality of active ingredients at the same time, while having excellent trapping power of active ingredients, stability of liposomes, and skin penetration, which can realize improved skin improvement effects. is to do

Another aspect is to provide an external preparation for skin containing the liposome.

The object of the present invention is not limited to the technical problem as described above, and another technical problem may be derived from the following description.

One aspect is a physiologically active substance; A carbon material containing at least one selected from the group consisting of fullerene and fullerol; Surfactants; And lipids; It provides a cosmetic composition comprising a liposome containing.

The cosmetic composition containing the liposome contains 0.0001 to 1.0% by weight of the bioactive material, 0.001 to 3.0% by weight of the carbon material, 0.001 to 1.0% by weight of the surfactant and 0.2 to 5% by weight of the lipid based on the total weight of the cosmetic composition. can include

The cosmetic composition including the liposome may include the bioactive material and the carbon material in a weight ratio of 0.01 to 1:1. The bioactive material may include a whitening component, an antioxidant component, an anti-wrinkle component, a moisturizing component, and an anti-inflammatory component. It may include any one or more selected from the group consisting of components, skin regeneration components, and skin aging inhibitory components.

The whitening component may include at least one selected from the group consisting of niacinamide, adenosine, and retinoid-based components.

The surfactant is polyglyceryl-3methylglucose distearate, glucolipid, lysolecitin, sorbitan stearate, glyceryl oleate, glyceryl stearate, polyoxyalkylene alkyl ether, polyoxyalkylene fatty acid ester, Alkyl poly glucoside, sodium lauryl ether sulfate, stearyl trimethyl ammonium chloride, alkyl polyglyceryl ether, stearyl trimethyl ammonium chloride, lauryl trimethyl ammonium chloride, distearyl dimethyl-ammonium chloride, poly(N,N'-dimethyl -3,5-methylene piperidinium) chloride, cetylpyridinium chloride, alkyltetraammonium salt, alkyldimethylbenzylammonium salt, alkylisoquinolinium salt, dialkyl-morpholinium salt, polyamine fatty acid derivative, amyl alcohol fatty acid derivative, benzal It may include at least one selected from the group consisting of conium chloride, benzethonium chloride, and potassium cetyl phosphate.

The lipid is soybean lecithin, egg yolk lecithin, hydrogenated lecithin, hydrogenated lysolecithin, hydrooxylated lecithin, tocopherol, tocopheryl acetate, aluminum/magnesium hydroxide stearate, pentaerythrityltetradi-t- It may include at least one selected from the group consisting of butyl hydroxyhydrocinnamate and capric triglyceride.

The cosmetic composition containing the liposome may further include at least one selected from the group consisting of polyol, oil, preservative and purified water.

Another aspect is to provide a composition for external application for skin containing the liposome.

The composition of one aspect is excellent in trapping active ingredients including a whitening component including niacinamide and a carbon material including at least one selected from the group consisting of fullerene and fullerol, and the stability and skin penetration of liposomes are remarkably good. can be excellent

Accordingly, the composition of the present embodiment can have excellent skin improvement effects by simultaneously implementing various functions such as whitening and wrinkle improvement.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily practice the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

Terms or words used in the specification and claims of the present invention are not limited to the usual or dictionary meaning, and the inventor can properly define the concept of the term in order to explain his/her invention in the best way. Based on the principle, it should be interpreted as a meaning and concept consistent with the technical idea of the present invention.

Throughout the specification of the present invention, when a part "includes" a certain component, it means that it may further include other components, not excluding other components unless otherwise stated. .

Throughout the specification of the present invention, "A and/or B" means A or B, or A and B.

Although the present invention has been specifically described below, the present invention is not limited thereto.

One aspect provides a cosmetic composition comprising a liposome having excellent trapping ability of an active ingredient.

In one embodiment, a physiologically active substance; A carbon material containing at least one selected from the group consisting of fullerene and fullerol; Surfactants; And lipids; It provides a cosmetic composition comprising a liposome containing.

In one embodiment, the liposome may be a spherical vesicle of a lipid double layer including a lipid and a surfactant, and a carbon material or a physiologically active material may be supported inside the spherical vesicle.

The diameter of the liposome is 5 to 1,500 nm, for example, 5 to 1,000 nm, 10 to 1,500 nm, 10 to 1,000 nm, 10 to 800 nm, 10 to 500 nm, 5 to 500 nm, 5 to 300 nm, 10 to 300 nm, 5 to 200 nm, 5 to 100 nm, or 10 to 100 nm.

The cosmetic composition containing liposomes of one embodiment can implement various effects such as whitening and wrinkle improvement by including a plurality of active ingredients such as physiologically active substances and carbon materials. In particular, the cosmetic composition containing the liposome of one embodiment has excellent trapping ability of active ingredients, excellent stability of the liposome itself, and good skin penetration rate, so that the skin improvement effect may be excellent.

Hereinafter, the components of the cosmetic composition containing liposomes will be described in detail.

A cosmetic composition containing a liposome of one embodiment may include a physiologically active substance, for example, a whitening component, an antioxidant component, a wrinkle improvement component, a moisturizing component, an anti-inflammatory component, a skin regeneration component, or a skin aging inhibitory component.

As used herein, the term "physiologically active substances" means substances capable of imparting useful activities to a subject, for example, skin regeneration, skin anti-aging, skin wrinkles, skin whitening, skin moisturizing, and the like. can

The bioactive material may be supported in the liposome.

The whitening component of one embodiment may include all known components capable of implementing a whitening effect.

Specifically, the whitening component of one embodiment may include one or more selected from the group consisting of niacinamide, adenosine, and retinoid-based components. More specifically, the whitening component of one embodiment may include niacinamide, but is not limited thereto.

At this time, the retinoid-based component means a derivative of vitamin A, and for example, retinol, retinal, retinoic acid, retinyl acetate, retinyl propionate ( retinyl propionate), retinyl linoleate, and retinyl palmitate.

The cosmetic composition containing the liposome of one embodiment may include 0.0001 to 1.0% by weight, specifically 0.0003 to 0.1% by weight of the physiologically active material based on the total weight of the cosmetic composition. A cosmetic composition containing liposomes of one embodiment includes a physiologically active substance, but when the physiologically active substance is included in an amount of 0.0001 to 1.0% by weight, the liposome of an appropriate size maintains a stable state for a long period of time while maintaining active ingredient capture rate and skin penetration rate And the skin improvement effect may be more excellent.

If the cosmetic composition containing the liposome of one embodiment contains less than 0.0001% by weight of the physiologically active material based on the total weight, the skin improvement effect is greater than when the cosmetic composition contains 0.0001 to 1.0% by weight of the physiologically active material. may be lowered On the other hand, when the cosmetic composition containing the liposome of one embodiment contains more than 1.0% by weight of the physiologically active material based on the total weight, the stability of the liposome is higher than when the cosmetic composition contains 0.0001 to 1.0% by weight of the physiologically active material. decrease, and the capture rate and skin penetration rate of active ingredients are reduced, and the degree of improvement of the skin improvement effect may be insignificant.

A cosmetic composition comprising a liposome of one embodiment includes a carbon material.

The cosmetic composition containing liposomes of one embodiment may include a carbon material containing at least one selected from the group consisting of fullerene and fullerol.

The fullerene refers to a molecule in which carbon atoms are arranged in a spherical, ellipsoidal, or cylindrical shape, and generally means a spherical carbon material, and is known to have antioxidant, whitening, and anti-wrinkle effects. In addition, the fullerol is obtained by introducing a hydroxyl group into fullerene, and is also called hydroxylated fullerene.

The carbon material of one embodiment may include each of the above fullerenes or fullerols alone.

Specifically, the carbon material of one embodiment may include both the fullerene and the fullerol. In particular, when the carbon material of one embodiment includes both the fullerene and the fullerol, the capture rate of the active ingredient of the liposome is better than when the fullerene or the fullerol is included alone, and the stability and skin penetration rate of the liposome are improved. It is excellent, and the skin improvement effect can be improved.

More specifically, the carbon material of one embodiment includes both the fullerene and the fullerol, and may include the fullerene and the fullerol in a weight ratio of 0.1 to 10:1. In particular, when the carbon material of one embodiment includes the fullerene and the fullerol in a weight ratio of 0.1 to 10: 1, the liposome has a good capture rate of active ingredients, and the liposome has excellent stability and skin penetration, so that the skin improvement effect is obtained. can be significantly improved.

Most specifically, the carbon material of one embodiment includes both the fullerene and the fullerol, and may include the fullerene and the fullerol in a weight ratio of 1 to 3:1. In particular, when the carbon material of one embodiment includes the fullerene and the fullerol in a weight ratio of 1 to 3: 1, the liposome has a good capture rate of active ingredients, excellent liposome stability and skin penetration, and has a skin improvement effect. can be significantly better.

The cosmetic composition containing the liposome of one embodiment may include 0.001 to 3.0% by weight, specifically 0.01 to 2% by weight of the carbon material based on the total weight of the composition. In particular, when the cosmetic composition containing liposomes of one embodiment includes carbon materials in an amount of 0.001 to 3.0% by weight based on the total weight, the active ingredient capture rate and skin penetration rate while maintaining a stable state for a long period of time while keeping the liposomes of an appropriate size stable. And the skin improvement effect may be more excellent.

If the cosmetic composition containing the liposome of one embodiment contains less than 0.001% by weight of the carbon material based on the total weight, the capture rate of the active ingredient and The stability and skin permeation rate of liposomes are lowered, so the degree of improvement in the skin improvement effect may be insignificant. On the other hand, when the cosmetic composition containing the liposome of one embodiment contains more than 3.0% by weight of the carbon component based on the total weight, the stability of the liposome is lowered than when the carbon material is included in 0.001 to 3.0% by weight. , the capture rate and skin penetration rate of active ingredients are lowered, so the degree of improvement in the skin improvement effect may be insignificant.

The cosmetic composition containing liposomes of one embodiment may include the bioactive material and the carbon material in a weight ratio of 0.01 to 1: 1, in which case, liposomes of an appropriate size are formed and can maintain a stable state for a long period of time. However, the active ingredient capture rate and skin penetration rate of liposomes are excellent, so the skin can be effectively improved. On the other hand, when the bioactive material and the carbon material are not included in a weight ratio of 0.01 to 1: 1, the stability of the liposome is lowered and the skin improvement effect is lowered due to the remaining physiologically active material that is not captured in the liposome. There may be a problem.

A cosmetic composition comprising the liposome of one embodiment includes a surfactant.

The cosmetic composition containing the liposome of one embodiment is polyglyceryl-3methylglucose distearate, glucolipid, lysolecitin, sorbitan stearate, glyceryl oleate, glyceryl stearate, polyoxyalkylene alkyl ether, poly Oxyalkylene fatty acid ester, alkyl polyglucoside, sodium lauryl ether sulfate, stearyl trimethyl ammonium chloride, alkyl polyglyceryl ether, stearyl trimethyl ammonium chloride, lauryl trimethyl ammonium chloride, distearyl dimethyl-ammonium chloride, poly( N,N′-dimethyl-3,5-methylenepiperidinium) chloride, cetylpyridinium chloride, alkyltetraammonium salt, alkyldimethylbenzylammonium salt, alkylisoquinolinium salt, dialkyl-morpholinium salt, polyamine fatty acid derivative, amyl It may include a surfactant including at least one selected from the group consisting of alcohol fatty acid derivatives, benzalkonium chloride, benzethonium chloride, and potassium cetyl phosphate.

Specifically, the surfactant of one embodiment may include polyglyceryl-3methylglucose distearate. In particular, the polyglyceryl-3methylglucose distearate is an edge activator component, so that the active ingredient can easily enter the liposome to increase the capture rate of the active ingredient, while increasing the elasticity of the liposome to realize excellent skin permeability. can do.

The cosmetic composition containing the liposome of one embodiment may include 0.001 to 1.0% by weight, specifically 0.01 to 0.5% by weight of a surfactant based on the total weight of the cosmetic composition. In particular, when the cosmetic composition containing liposomes of one embodiment includes a surfactant in an amount of 0.001 to 1.0% by weight based on the total weight, the active ingredient capture rate and skin penetration rate while maintaining a stable state for a long period of time while maintaining a stable state of liposomes of appropriate size And the skin improvement effect may be more excellent.

If the cosmetic composition containing the liposome of one embodiment contains less than 0.001% by weight of the surfactant based on the total weight, the capture rate of the active ingredient and The stability and skin permeation rate of liposomes are lowered, so the degree of improvement in the skin improvement effect may be insignificant. On the other hand, when the cosmetic composition containing the liposome of one embodiment contains more than 1.0% by weight of the surfactant based on the total weight, the stability of the liposome is lowered than when the surfactant is included in 0.001 to 1.0% by weight. , the capture rate and skin penetration rate of active ingredients are lowered, so the degree of improvement in the skin improvement effect may be insignificant.

A cosmetic composition containing a liposome of one embodiment includes a lipid.

The cosmetic composition comprising the liposome of one embodiment is soybean lecithin, egg yolk lecithin, hydrogenated lecithin, hydrogenated lysolecithin, hydroxylated lecithin, tocopherol, tocopheryl acetate, aluminum/magnesium hydroxide stearate, pentagram A lipid containing at least one selected from the group consisting of erythrityltetra-di-t-butylhydroxyhydrocinnamate and capric triglyceride may be included.

Specifically, the cosmetic composition containing the liposome of one embodiment may be a phospholipid, wherein the phospholipid is selected from the group consisting of soybean lecithin, egg yolk lecithin, hydrogenated lecithin, hydrogenated lysolecithin and hydroxylated lecithin may contain one or more.

The cosmetic composition containing the liposome of one embodiment may include 0.2 to 5.0% by weight of lipid, specifically 0.2 to 1.0% by weight based on the total weight of the composition. In particular, when the cosmetic composition containing liposomes of one embodiment includes lipids in an amount of 0.2 to 5.0% by weight based on the total weight, the liposomes of appropriate size maintain a stable state for a long period of time while maintaining active ingredient capture rate, skin penetration rate, and The skin improvement effect may be more excellent.

If the cosmetic composition containing the liposome of one embodiment contains less than 0.2% by weight of the lipid based on the total weight, than the case where the lipid is contained in 0.2 to 5.0% by weight, the capture rate of the active ingredient and the Stability and skin penetration rate are lowered, and the degree of improvement of the skin improvement effect may be insignificant. On the other hand, when the cosmetic composition containing the liposome of one embodiment contains more than 5.0% by weight of lipid based on the total weight, the stability of the liposome is lowered than when it contains 0.2 to 5.0% by weight of the lipid, and the effective The collection rate and skin penetration rate of components are lowered, so the degree of improvement in the skin improvement effect may be insignificant.

The cosmetic composition comprising the liposome of one embodiment may further include a polyol.

In more detail, the cosmetic composition containing the liposome of one embodiment may include 0.01 to 60.0% by weight of polyol, specifically 1 to 40.0% by weight, but is not limited thereto.

The polyol of one embodiment may include all known polyols. For example, the polyol may include butylene glycol, glycerin, glycerol, propanol, 1,2hexanediol, 1,3-butylene glycol, and propylene glycol. It may include one or more selected from the group consisting of lycol, dipropylene glycol, isoprene glycol, sorbitol, xylitol, erythritol, fructose, lactose, mannitol, glucose, sucrose, maltose, and maltitol, but is not limited thereto. don't

The cosmetic composition comprising the liposome of one embodiment may further include oil.

In more detail, the cosmetic composition containing the liposome of one embodiment may include 0.0001 to 10% by weight of oil, specifically 0.001 to 5% by weight of oil, but is not limited thereto.

The oil of one embodiment may include all known oils, for example, mango butter oil, shea butter oil, cocoa seed butter oil, macadamia nut oil, olive oil, meadowfoam seed oil, refined grape seed oil, rosehip Oil, safflower oil, peach seed oil, sunflower seed oil, macadamia seed oil, caprylyl carbonate, squalane, neopentyl glycol diheptanoate, tocopheryl acetate, trioctanoin, C12-15 alkylethylhexane Noate, Octyldodecyl Myristate, Tricaprylin, Octyldodecyl Stearoyl Stearate, Bis-Hydroxyethoxypropyl Dimethicone, Caprylic/Capric Triglyceride, Isotridecyl Isononanoate, Polyglyceryl-2triisostearate, Diisostearyl Maleate, Dipentaerythrite Fatty Acid Ester, Cetylethylhexanoate, Cetyloctanoate, Ozokerite, Glyceryl Trioctanoate, Octyldodecyl Myristate, isopropyl palmitate, isopropyl myristate, octyl palmitate, phenyltrimethicone, cyclomethicone, dimethicone, decamethylcyclopentasiloxane hydrocarbon oil, hydrogenated PEG-60 castor oil, hydrogenated It may include one or more selected from the group consisting of tid polyisobutene and hydrogenated polydecene, but is not limited thereto.

The cosmetic composition comprising the liposome of one embodiment may further include a preservative.

In more detail, the cosmetic composition containing the liposome of one embodiment may include a preservative at 0.1 to 10% by weight, specifically 0.5 to 2% by weight, but is not limited thereto.

Preservatives of one embodiment may include all known preservatives, and may include, for example, at least one selected from the group consisting of 1,2-hexanediol and pentylene glycol, but is not limited thereto.

The cosmetic composition of one embodiment may further include excipients or additives necessary for formulation of each formulation in addition to the above components. For example, the excipients or additives include surfactants, emulsifiers, emulsifiers, silicone polymers, pigments, colorants, fragrances, preservatives, bactericides, oxidation stabilizers, organic solvents, softeners, antioxidants, free radical destroyers, opacifiers, As a stabilizer, emollient, antifoam, moisturizer, vitamin, insect repellent, preservative, anti-inflammatory, filler, polymer, propellant, alkalizing agent, acidifying agent, colorant, thickening agent, skin conditioning agent, whitening agent, antioxidant, anti-wrinkle agent and solubilizing agent It may include one or more selected from the group consisting of, but is not limited thereto.

The cosmetic composition of one embodiment consists of softening lotion, nutrient lotion, astringent lotion, toner, lotion, essence, serum, cream, massage cream, pack, makeup base, BB cream, foundation, powder, cleansing foam, cleansing cream and cleansing water It may be one formulation selected from the group, but is not limited thereto.

Another aspect is a bioactive substance; A carbon material containing at least one selected from the group consisting of fullerene and fullerol; Surfactants; And a lipid; It provides a composition for external application for skin comprising a liposome containing.

The bioactive material, carbon material, surfactant, lipid and liposome are as described above.

The external skin preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The external skin preparation is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, water-based components, oil-based components, powder components, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, and fragrances. , colorants, various skin nutrients, or combinations thereof and may be suitably blended as needed.

Hereinafter, the liposomal cosmetic composition having excellent trapping ability of the active ingredient of the present invention will be described in detail through Examples, Comparative Examples, and Experimental Examples. Since these examples are intended to illustrate the present invention only, the scope of the present invention is not to be construed as being limited by these examples.

[Production Example]

Preparation 1: Evaluation according to the ratio of whitening ingredients and carbon materials

A liposome cosmetic composition was prepared by mixing the whitening component, carbon material, surfactant, lipid, polyol, oil, preservative and purified water according to Table 1 [unit: weight%].

At this time, in Table 1, the total amount of the whitening component and the carbon material (including fullerene and fullerol in a weight ratio of 1.5: 1) was 0.04% by weight, and their mixing ratio was mixed according to Table 2 [unit: weight ratio].

In addition, niacinamide was used as a whitening component, polyglyceryl-3methylglucose distearate (trade name: TEGOCARE 450) was used as a surfactant, and phospholipid was used as a lipid.

		Example 1, Example 2 and Comparative Example 1
whitening ingredient		0.04
carbon material	fullerene
	fuller roll
Surfactants		0.1
lipids		1.0
polyol		40
oil		0.001
antiseptic		0.01
Purified water		to. 100

	Example 1	Example 2	Comparative Example 1
whitening ingredients	One	0.01	1.2
carbon material	One	One	One

Manufacture 2: Evaluation according to the use of fullerene and fullerol

The whitening component, carbon material, surfactant, lipid, polyol, oil, preservative and purified water were mixed according to Table 3 [unit: weight%], except that the liposome composition was prepared, and carried out in the same manner as in Example 1. did

At this time, in Table 3, the mixing ratio of fullerene and fullerol constituting the carbon material was mixed according to Table 4 [unit: weight ratio], and in Table 4, the mixing ratio of fullerene and fullerol in Example 1 was also described.

		Examples 3 to 7
whitening ingredient		0.02
carbon material	fullerene	0.02
	fuller roll
Surfactants		0.1
lipids		1.0
polyol		40
oil		0.001
antiseptic		2.0
Purified water		to. 100

	Example 1	Example 3	Example 4	Example 5	Example 6	Example 7
fullerene	1.5	-	0.11	0.67	9	One
floral	One	One	One	One	One	-

[Experimental example]

Experimental Example 1: Evaluation of capture rate of niacinamide

In the liposomal cosmetic compositions of Examples 1 to 7 and Comparative Example 1, the niacinamide capture rate was evaluated, and the results are shown in Table 5 [Unit: %].

Specifically, the capture rate of the niacinamide is obtained by scooping the supernatant after centrifugation, checking the niacinamide concentration (remaining niacinamide) by HPLC, and using this, 'capture rate (%) = (initial niacinamide content - supernatant) Niacinamide contained in)/(initial niacinamide content) * 100%' was calculated.

	Capture rate (%)		Capture rate (%)
Example 1	100	Example 5	95
Example 2	100	Example 6	92
Example 3	88	Example 7	89
Example 4	95	Comparative Example 1	100 (uncaptured niacinamide residue)

In Table 5, looking at Example 1, Example 2 and Comparative Example 1, when the liposomal cosmetic composition includes a whitening component and a carbon material in a weight ratio of 0.01 to 1: 1, the whitening component remaining without being captured in the liposome It can be seen that the collection rate of the whitening component is 100%, which is very excellent. In particular, in the case of Comparative Example 1, since the whitening component is included in an excessive amount compared to the carbon material, unencapsulated niacinamide remains, and thus, unencapsulated niacinamide may not be effectively absorbed into the skin and may not be economical. In addition, the niacinamide component may be exposed to the outside and cause problems such as discoloration or oxidation, and may cause skin irritation.

In addition, when comparing Example 1 and Examples 3 to 7, it can be seen that the collection rate is as high as 88% or more even when the carbon material includes fullerene or fullerol alone, and preferably the carbon material is fullerene and fullerol. It can be seen that the collection rate is further improved to 92% or more when all of the fullerols are included. In particular, it can be seen that when the mixing ratio of fullerene and fullerol in the carbon material is 3:2, the whitening component capture rate is the best at 100%.

[Production Example]

Manufacture 3: Evaluation according to the type of carbon material

A liposome cosmetic composition was prepared by mixing the whitening component, carbon material, surfactant, lipid, polyol, oil, preservative and purified water according to Table 6 [unit: weight%].

In addition, niacinamide was used as a whitening component, polyglyceryl-3methylglucose distearate (trade name: TEGOCARE 450) was used as a surfactant, and phospholipid was used as a lipid.

		Example 8	Example 9	Example 10
whitening ingredient		0.01	0.01	0.01
carbon material	fullerene	0.006	0.01	-
	fuller roll	0.004	-	0.01
Surfactants		0.1	0.1	0.1
lipids		1.0	1.0	1.0
polyol		40.0	40.0	40.0
oil		0.001	0.001	0.001
antiseptic		2.0	2.0	2.0
Purified water		to. 100

Preparation 4: Evaluation according to lipid content

In the same manner as in Example 8, except that the liposome cosmetic composition was prepared by mixing the whitening component, carbon material, surfactant, lipid, polyol, oil, preservative and purified water according to Table 7 [unit: weight%]. conducted.

At this time, the contents of the composition of Example 8 were also described in Table 7.

		Example 8	Example 11	Comparative Example 2
whitening ingredient		0.01	0.01	0.01
carbon material	fullerene	0.006	0.006	0.006
	fuller roll	0.004	0.004	0.004
Surfactants		0.1	0.1	0.1
lipids		1.0	1.3	0.16
polyol		40.0	40.0	40.0
oil		0.001	0.001	0.001
antiseptic		2.0	2.0	2.0
Purified water		to. 100

Preparation 5: Evaluation according to surfactant content

In the same manner as in Example 8, except that the liposome cosmetic composition was prepared by mixing the whitening component, carbon material, surfactant, lipid, polyol, oil, preservative and purified water according to Table 8 [Unit:% by weight]. conducted.

At this time, the contents of the composition of Example 8 are also described in Table 8.

		Example 8	Comparative Example 3	Comparative Example 4	Comparative Example 5
whitening ingredient		0.01	0.01	0.01	0.01
carbon material	fullerene	0.006	0.006	0.006	0.006
	fuller roll	0.004	0.004	0.004	0.004
Surfactants		0.1	0.0002	-	2.0
lipids		0.2	0.2	0.2	0.5
polyol		40.0	40.0	40.0	40.0
oil		0.001	0.001	0.001	0.001
antiseptic		2.0	2.0	2.0	2.0
Purified water		to. 100

Manufacture 6: Evaluation according to ingredient inclusion

In the same manner as in Example 8, except that the liposome cosmetic composition was prepared by mixing the whitening component, carbon material, surfactant, lipid, polyol, oil, preservative and purified water according to Table 9 [Unit:% by weight]. conducted.

At this time, the contents of the composition of Example 8 were also described in Table 9.

		Example 8	Comparative Example 6	Comparative Example 7	Comparative Example 8	Comparative Example 9	Comparative Example 10	Comparative Example 11	Comparative Example 12
whitening ingredient		0.01	0.01	-	0.01	-	-	0.01	0.01
carbon material	fullerene	0.006	-	0.006	-	0.006	-	0.01	-
	fuller roll	0.004	-	0.004	-	0.004	-	-	0.01
Surfactants		0.1	0.1	0.1	0.1	0.1	0.1	-	-
lipids		1.0	1.0	1.0	-	-	1.0	1.0	1.0
polyol		40.0	40.0	40.0	40.0	40.0	40.0	40.0	40.0
oil		0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001
antiseptic		2.0	2.0	2.0	2.0	2.0	2.0	2.0	2.0
Purified water		to. 100

[Experimental example]

Experimental Example 2: Evaluation of physical properties

In the compositions according to the above Examples and Comparative Examples, the particle size and elasticity values of the liposomes were evaluated, and the results are shown in Table 10.

According to the following variability test, the particle size (unit: nm) and elasticity of the liposomes were evaluated.

Specifically, the ability of the elastic liposome to pass through the artificial permeability barrier was measured using a mini extruder. In more detail, after applying a pressure of 0.2 MPa to the elastic liposome for 1 minute and 30 seconds, the amount of elastic liposome passing through a polycarbonate membrane having pores of 0.08 μm was measured, and the particle size of the liposome passing through it was measured. .

At this time, the elasticity of the elastic liposome membrane is proportional to the following formula.

[Equation 1] Deformability index = JFlux X (rv/rp)2

- JFlux: The amount of elastic liposomes passing through the membrane

- rv: Liposome particle size after extrusion

- rp: pore size of membrane

In addition, '-' in Table 10 means a state in which it is difficult to measure the particle size itself due to the non-uniform and unstable state of the liposome.

	particle size	elastic figure		particle size	elastic figure
Example 8	257	23.1	Comparative Example 7	245	22.9
Example 9	232	18.1	Comparative Example 8	-	-
Example 10	236	18.2	Comparative Example 9	-	-
Example 11	271	17.2	Comparative Example 10	224	8.9
Comparative Example 2	-	-	Comparative Example 11	-	-
Comparative Example 3	248	14.8	Comparative Example 12	-	-
Comparative Example 4	263	12.6
Comparative Example 5	-	-
Comparative Example 6	220	8.5

Looking at Table 10, it can be seen that according to the present embodiment, the particle size is formed at an appropriate level and the elasticity value is excellent.

Experimental Example 3: Stability evaluation

In the compositions according to the above Examples and Comparative Examples, stability of liposomes was evaluated, and the results are shown in Table 11.

Specifically, the stability of the liposome was evaluated according to the following items while observing for 4 weeks by measuring the particle size of the liposome every week according to Experimental Example 2 described above.

*'○': There is almost no change in particle size until about 4 weeks pass, and it is uniform

state of being maintained

* '△': From about the 2nd week, the particle size changes and becomes non-uniform

* '-': Due to the non-uniformity and unstable state, it is difficult to measure the particle size itself, and it is difficult to evaluate the stability of the liposome itself.

	Liposome stability		Liposome stability
Example 8	○	Comparative Example 7	○
Example 9	○	Comparative Example 8	-
Example 10	○	Comparative Example 9	-
Example 11	○	Comparative Example 10	○
Comparative Example 2	-	Comparative Example 11	-
Comparative Example 3	△	Comparative Example 12	-
Comparative Example 4	△
Comparative Example 5	-
Comparative Example 6	○

In the case of Comparative Example 9 in the above experiment, the liposome was not stably dispersed.

That is, according to this embodiment, liposomes are stably and uniformly dispersed, and there is no precipitation of carbon materials, which means that excellent liposome stability can be realized.

Experimental Example 4: Evaluation of skin penetration rate

After measuring the skin penetration rate of niacinamide in the compositions according to the above Examples and Comparative Examples, the results are shown in Table 12 [Unit: %].

Specifically, the skin penetration rate was evaluated by performing a transdermal absorption diffusion experiment (Franz diffusion cell).

First, after cutting the skin (area: 0.6362 cm ² ) on which the cosmetic composition was deposited with scissors, 5 mL of 100% methanol was added, and crushed for 5 minutes with an ultrasonic disruptor, and then filtered through a 0.45 μm filter. Niacinamide content was determined by HPLC.

After that, the skin penetration rate (%) was calculated as 'skin penetration rate (%) = (niacinamide content measured in the skin / niacinamide content contained in the deposited cream) X 100 (%)', and the results are shown in the table 12.

	Niacinamide skin penetration rate (%)		Niacinamide skin penetration rate (%)
Example 8	44.6	Comparative Example 7	-
Example 9	29.1	Comparative Example 8	10.2
Example 10	23.5	Comparative Example 9	-
Example 11	26.2	Comparative Example 10	-
Comparative Example 2	-	Comparative Example 11	-
Comparative Example 3	16.2	Comparative Example 12	-
Comparative Example 4	10.3
Comparative Example 5	-
Comparative Example 6	19.3

Looking at Table 12, it can be seen that according to this example, the skin penetration rate of the active ingredient is remarkably excellent.

Experimental Example 5: Evaluation of wrinkle and whitening improvement effect

The whitening and wrinkle improvement efficacies of the compositions of Examples 8 to 10 and Comparative Examples 6 to 10 were evaluated, and the results are shown in Table 13 [Unit: score].

In addition, as a control, a cosmetic composition (liposome X) in which 40% by weight of polyol, 2% by weight of preservative, and the remaining amount of purified water was mixed was used.

At this time, in the experiment, a group of 30 people (subject: women in their 20s and 30s) were asked to apply the liposome cosmetic composition of Example 8 to the facial skin including the eye area morning and evening for a total of 4 weeks, then, The degree of whitening and wrinkle improvement was evaluated by the following scores, and the average values are shown in Table 13. In addition, in the same manner as in Example 8, experiments using other Examples, Comparative Examples and Controls were conducted, and the results are shown in Table 13.

- 5 points: very good

- 4 points: excellent

- 3 points: Fair

- 2 points: Poor

- 1 point: very bad

	whitening evaluation	Wrinkle improvement evaluation
Example 8	4.85	4.75
Example 9	4.20	3.91
Example 10	4.10	3.98
Example 11	4.55	4.05
Comparative Example 2	3.01	2.89
Comparative Example 3	3.00	2.87
Comparative Example 4	2.95	2.75
Comparative Example 5	3.24	3.05
Comparative Example 6	3.34	3.01
Comparative Example 7	3.21	3.20
Comparative Example 8	3.42	3.02
Comparative Example 9	3.12	3.24
Comparative Example 10	2.98	2.88
Comparative Example 11	2.90	2.82
Comparative Example 12	2.90	2.81
control group	2.88	2.78

Looking at Table 13, it can be seen that according to the present embodiment, the whitening and wrinkle improvement effects are remarkably excellent.

On the other hand, through Comparative Example 10 and the control group, which did not contain a whitening component and a carbon material, it was confirmed that the basic cosmetic component (polyol) and the liposome raw material had no significant effect on whitening and wrinkle improvement.

[Production Example]

Manufacture 7: Evaluation of carbon material capture rate in liposomes in response to carbon material content changes

According to Table 14, a liposome cosmetic composition containing a carbon material was prepared.

At this time, as the carbon material in Table 14, a mixture of fullerene and fullerol at a weight ratio of 1.5:1 was used.

	Example 12	Comparative Example 13
whitening ingredient	0.5	0.5
carbon material	0.5	0.5
Surfactants	0.1	-
lipids	1.0	1.0
polyol	40.0	40.0
oil	0.001	0.001
antiseptic	2.0	2.0
Purified water	to 100

[Experimental example]

Experimental Example 6: Evaluation of carbon material capture rate in liposomes

In the liposome cosmetic compositions of Example 12 and Comparative Example 13, the collection rate of carbon materials was evaluated, and the results are shown in Table 15 [Unit: %].

At this time, the capture rate of the carbon material was carried out in the same manner as the capture rate of niacinamide in Experimental Example 1.

	Capture rate (%)		Capture rate (%)
Example 12	100	Comparative Example 13	75

Looking at Tables 14 and 15, it can be seen that according to the present embodiment, all carbon materials are trapped in the liposome.

On the other hand, in the case not according to this embodiment, it can be seen that the remaining carbon material is increased without being trapped in the liposome. That is, when the present embodiment is not followed, as the carbon material is not effectively absorbed into the skin, the skin improvement effect may not be sufficient.

As a result, in view of the above information, according to this embodiment, it is possible to provide a liposome cosmetic composition in a stable state by being formed in an appropriate size while having excellent trapping power of whitening ingredients and carbon materials. Accordingly, the whitening and anti-wrinkle effect of the liposome cosmetic composition may be remarkably excellent.

As described above, the description of the present invention is for illustrative purposes, and those skilled in the art to which the present invention pertains can easily be modified into other specific forms without changing the technical spirit or essential features of the present invention. You will understand. Therefore, the embodiments described above are illustrative in all respects, and should be understood as non-limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims rather than the detailed description above, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be construed as being included in the scope of the present invention. do.

Claims (9)

1. bioactive substances;

   A carbon material containing at least one selected from the group consisting of fullerene and fullerol;

   Surfactants; and

   A cosmetic composition comprising a liposome comprising a lipid;
2. According to claim 1,

   The cosmetic composition

   A cosmetic composition comprising 0.0001 to 1.0% by weight of the bioactive material, 0.001 to 3.0% by weight of the carbon material, 0.001 to 1.0% by weight of the surfactant and 0.2 to 5% by weight of the lipid based on the total weight of the cosmetic composition. .
3. According to claim 1,

   The cosmetic composition

   The cosmetic composition comprising the physiologically active material and the carbon material in a weight ratio of 0.01 to 1: 1.
4. According to claim 1,

   The physiologically active substance is any one or more selected from the group consisting of a whitening component, an antioxidant component, an anti-wrinkle component, a moisturizing component, an anti-inflammatory component, a skin regeneration component, and a skin aging inhibitory component, a cosmetic composition.
5. According to claim 4,

   The whitening ingredient

   A cosmetic composition comprising at least one selected from the group consisting of niacinamide, adenosine, and retinoid-based components.
6. According to claim 1,

   The surfactant is

   Polyglyceryl-3methylglucose distearate, glucolipid, lysolecithin, sorbitan stearate, glyceryl oleate, glyceryl stearate, polyoxyalkylene alkyl ether, polyoxyalkylene fatty acid ester, alkyl polyglucoside, Sodium lauryl ether sulfate, stearyl trimethyl ammonium chloride, alkyl polyglyceryl ether, stearyl trimethyl ammonium chloride, lauryl trimethyl ammonium chloride, distearyl dimethyl-ammonium chloride, poly(N,N'-dimethyl-3,5 -Methylenepiperidinium) chloride, cetylpyridinium chloride, alkyltetraammonium salt, alkyldimethylbenzylammonium salt, alkylisoquinolinium salt, dialkyl-morpholinium salt, polyamine fatty acid derivative, amyl alcohol fatty acid derivative, benzalkonium chloride , A cosmetic composition comprising at least one selected from the group consisting of benzethonium chloride and potassium cetyl phosphate.
7. According to claim 1,

   The lipid is

   Soy Lecithin, Egg Yolk Lecithin, Hydrogenated Lecithin, Hydrogenated Lysolecithin, Hydroxylated Lecithin, Tocopherol, Tocopheryl Acetate, Aluminum/Magnesium Hydroxide Stearate, Pentaerythrityltetra-di-t-butylhydro A cosmetic composition comprising at least one selected from the group consisting of hydroxyhydrocinnamate and capric triglyceride.
8. According to claim 1,

   The cosmetic composition

   A cosmetic composition further comprising at least one selected from the group consisting of polyols, oils, preservatives and purified water.
9. bioactive substances;

   A carbon material containing at least one selected from the group consisting of fullerene and fullerol;

   Surfactants; and

   A composition for external application for skin comprising a liposome comprising a lipid.