WO2023003410A1 - Composition for autoimmune builder of plants and usage method using same - Google Patents

Composition for autoimmune builder of plants and usage method using same Download PDF

Info

Publication number
WO2023003410A1
WO2023003410A1 PCT/KR2022/010742 KR2022010742W WO2023003410A1 WO 2023003410 A1 WO2023003410 A1 WO 2023003410A1 KR 2022010742 W KR2022010742 W KR 2022010742W WO 2023003410 A1 WO2023003410 A1 WO 2023003410A1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
weight
composition
autoimmunity
plants
Prior art date
Application number
PCT/KR2022/010742
Other languages
French (fr)
Korean (ko)
Inventor
황대진
박상진
황순호
Original Assignee
농업회사법인 주식회사 아그로비즈
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 농업회사법인 주식회사 아그로비즈 filed Critical 농업회사법인 주식회사 아그로비즈
Publication of WO2023003410A1 publication Critical patent/WO2023003410A1/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/74Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
    • A01N43/781,3-Thiazoles; Hydrogenated 1,3-thiazoles

Definitions

  • the present invention forms an autoimmune enhancing composition of a plant in which aspirin (acetylsalicylic acid, ASA) and benzothiazole are dissolved together in water, and the enhancing agent composition is applied to the leaves, stems and fruits (fruits) of plants by a conventional spraying method. It is sprayed/provided on the surface and enhances the autoimmune function against external environmental stresses such as drought, soil salinity, freezing, cold resistance and heat during the plant growth process, ultimately improving the quality and quality of crops by improving resistance to disease. It relates to a plant autoimmunity enhancer composition containing active ingredients of aspirin and benzozoazole capable of increasing productivity.
  • aspirin acetylsalicylic acid, ASA
  • benzothiazole benzothiazole
  • Plants are constantly subjected to stress from the surrounding environment during their survival period from germination to death. Unlike animals, plants do not have the ability to move around to avoid such stress, so they have a sophisticated internal control system to actively cope with stress. need to establish
  • Plant stress management is a very urgent task for centuries, which faces problems such as the ever-increasing population and reduction of arable land.
  • climate change is the cause of the present Earth moving toward a situation that causes more stress to plants.
  • Korean Patent Publication No. 10-2021-0085328 A a plant-growth-promoting bacteria (PGPR) Pennybacillus Yongin Nsis A composition for inhibiting salt stress in ginseng containing a DCY84T (Paenibacillus yonginensis DCY84T) strain is proposed, and in Korea Patent Publication No. 10-2021-0069224 A, drought in plants containing the BrPP5 gene consisting of the nucleotide sequence of SEQ ID NO: 1 And a Chinese cabbage-derived gene having high temperature stress resistance and its use are proposed, and in Korea Patent Publication No. 10-2019-0133422, rice-derived OsCP1 (Oryza sativa cysteine proteinase 1) gene and its use is presented.
  • PGPR plant-growth-promoting bacteria
  • US Patent Publication No. 16/267910 proposes a composition for plant growth and resistance in order to stimulate germination in agricultural crops
  • US Patent Publication No. 2021-0180074 A1 discloses abiotic stress-resistant plants and abiotic Polynucleotides are proposed to improve stress and methods
  • US Patent Publication No. 2020-0095595 A1 suggests a method for producing high-yield crops under abiotic stress conditions
  • US Patent Publication No. 2020-0208053 A1 Suggests ways to reduce water stress on plants.
  • Japanese Unexamined Patent Publication No. 2021-093933 A proposes a method for imparting environmental stress resistance to plants by contacting active oxygen to plants on the expected date of flowering
  • Japanese Unexamined Patent Publication No. 2018-525440 discloses amide 7-carboxy Regulating plant growth, regulating plant metabolic processes, regulating plant physiological processes, or affecting plant biotic stress by plant stimulants including benzo[1,2,3]thiadiazoles and their derivatives It suggests an application method that can prevent or prevent the effects of abiotic stress on plants
  • Japanese Patent Publication No. 2018-511562 suggests a 2-oxo-3,4-dihydroquinoline compound as a plant growth regulator.
  • Japanese Patent Laid-open Publication No. 2017-137281 suggests a high temperature stress tolerance improver of plants containing allantoin as an active ingredient, a method for improving high temperature stress tolerance, a bleaching inhibitor and a DREB2A gene expression promoter.
  • the present application provides a plant autoimmunity enhancing composition in which aspirin (acetylsalicylic acid, ASA) and benzothiazole are dissolved together in water to the leaves, stems and fruit (fruit) surfaces of plants by spraying, Aspirin enhances the autoimmune function against external environmental stress such as drought, soil salinity, freezing, cold resistance and heat during the growth process, thereby suppressing infection and improving resistance to disease, thereby providing high quality and increased productivity of crops. It is an object of the invention to provide a plant autoimmunity enhancing composition containing active ingredients of and benzozoazole and a method of using the same.
  • aspirin acetylsalicylic acid, ASA
  • benzothiazole benzothiazole
  • the technology of the present invention is a plant autoimmunity enhancer composition as a means to achieve the above object, that is, to relieve the stress provided from the external environment during the crop cultivation process, in which 1.5 to 5.0% by weight of aspirin (acetylsalicylic acid, ASA) is contained in water. and 0.5 to 2.0% by weight of benzothiazole, 0.05 to 0.25% by weight of preservative, 0.45 to 3.5% by weight of penetrant, and 0.10 to 0.50% by weight of antifoaming agent. provided to achieve
  • the preservative is at least one preservative selected from sorbic acid, benzoic acid, paraoxybenzoic acid, propionic acid, methylparaben, propylparaben, phenoxyethanol, diazolidinyl urea, and imidazolidinyl urea.
  • the penetrant may be sodium naphthalenesulfonate-formalin condensate, sodium alkylnaphthalenesulfonate or its formalin condensate, formalin condensate of sodium phenolsulfonate and sodium naphtholsulfonate, sodium alkyl diphenyl ether disulfonate, sodium ligninsulfonate, b Ionic surfactant Polyoxyethylene alkyl (allyl) ether, sodium polyacrylate, olefin-sodium maleate copolymer, condensed phosphate, alkylbenzene (naphthalene) sulfonate, dioctylsulfone maleate sodium, styrene-maleic anhydride copolymer partial amide
  • One or more types of penetrant may be selected and used among esterification/partial esterification, and the antifoaming agent may be a silicone-based antifoaming agent composed mainly of
  • the present application is a method for enhancing autoimmunity of plants to relieve stress provided from the external environment during the crop cultivation process, by adding 1.5 to 5.0% by weight of aspirin (acetylsalicylic acid, ASA) and 0.5 to 2.0% by weight of benzozoazole in water.
  • aspirin acetylsalicylic acid, ASA
  • a plant autoimmunity enhancing composition wherein the autoimmune enhancing agent composition is 1/
  • manual and automated spraying equipment is used to spray the leaves, stems, and fruits of plants to enhance plant autoimmunity. is initiated
  • aspirin acetylsalicylic acid, ASA
  • ASA acetylsalicylic acid
  • benzothiazole is aimed at doubling the autoimmune function of aspirin to provide autoimmune function against external environmental stress, as well as activating the autoimmune function of stress against various external environments, It is desirable to purchase and use commercially available drugs without particular restrictions.
  • the preservative is to prevent deterioration in function and marketability of the composition, including preservation due to the occurrence of microorganisms or molds when the organic substances contained in the plant's autoimmune enhancing composition containing aspirin and benzozoazole are passed at an appropriate temperature and over a period of time.
  • the penetrant is used for the purpose of enhancing the penetration rate of the autoimmune enhancer active ingredients of aspirin and benzoxazole sprayed on the stem, leaf, and fruit (fruit) of the plant into the epidermal tissue of the plant stem, leaf, and fruit (fruit).
  • the antifoaming agent is used for the purpose of enhancing the effect of the autoimmune enhancer, and the antifoaming agent causes the autoimmune enhancer composition of the present application to generate a large amount of foam due to the penetrant included in the technical configuration of the present application. It is used for the purpose of preventing
  • the present invention is a plant autoimmunity enhancer composition containing active ingredients of aspirin and benzoxazole to improve the autoimmune function against external environmental stresses such as drought, soil salinity, freezing, cold tolerance and heat during plant growth.
  • An autoimmune enhancer composition of plants capable of providing high quality and increased productivity of crops by suppressing infection and improving resistance to diseases by enhancing plant autoimmunity and a method of using the same can sufficiently overcome a wider range of external environmental stress, so that all crops can be It is expected to be widely applied to farming households.
  • the plant autoimmunity enhancer of the present application can sufficiently solve the problems of various stresses encountered by plants due to changes in the external environment, and can produce a large number of high-quality crops, thereby greatly solving future food problems as well as quality It is expected that it will greatly contribute to the increase in farm household income due to the effect that can contribute to economic income for farm household production activities by maintaining high quality marketability by improvement.
  • the technology of the present invention is a technology for providing a plant autoimmune enhancing agent composition for relieving stress provided from the external environment during the crop cultivation process, in which aspirin (acetylsalicylic acid, ASA) is 1.5 to 5.0% by weight and benzozoazole ( Benzothiazole) is dissolved in 0.5 to 2.0% by weight, preservative is 0.05 to 0.25% by weight, penetrant is dissolved in 0.45 to 3.5% by weight, and antifoaming agent is dissolved in 0.10 to 0.50% by weight to form an autoimmune enhancing composition for plants and changes in the external environment.
  • aspirin acetylsalicylic acid, ASA
  • benzozoazole Benzothiazole
  • preservative is 0.05 to 0.25% by weight
  • penetrant is dissolved in 0.45 to 3.5% by weight
  • antifoaming agent is dissolved in 0.10 to 0.50% by weight to form an autoimmune enhancing composition for plants and changes in the external environment.
  • a plant autoimmunity enhancer composition capable of solving the problems of various stresses encountered by plants and yielding high-quality crops
  • the object of the present invention is a means of preparing an immune booster composition, diluting it with water to 1/200, and spraying the leaves, stems, and fruits of grains or fruits grown in a 660 m2 greenhouse using a sprinkler. can be achieved
  • Salicylic acid (Samjeon Pure Medicine) and glacial acetic acid were mixed, synthesized under the catalyst of phosphoric acid, and left in a refrigerator at 2 o C for one day to obtain an aspirin precipitate, which was then used as an aspirin source according to the following examples.
  • Table 1 shows the results of Comparative Examples 1 to 3 and Examples 1 to 3.
  • Example 1 9.83 - - Example 2 - 5.11 - Example 3 - - 336 Comparative Example 1 7.16 - - Comparative Example 2 - 3.52 - Comparative Example 3 - - 284
  • Examples 1 to 3 when the plant autoimmune enhancer composition was sprayed and coated on crops of watermelon, Chinese cabbage and grapes, the watermelon showed an average weight of 9.83 kg, and in the case of Chinese cabbage, the average weight was 5.11 kg per bag, In the case of grapes, 336 g per bunch is shown.
  • the composition composed of aspirin and benzozoazole, the active ingredients of the plant autoimmune enhancer of the present application is coated on the leaves, stems, and fruits of plants during the crop cultivation process, the outside It was confirmed that it was possible to grow crops of the highest quality by solving the stress on the environment.
  • the upper and lower limits of the raw material components described in the claims of the present application are set is that the effect provided by the composition is insignificant compared to the increase in the manufacturing cost of the immune enhancer composition when the upper limit is exceeded, and when applied below the lower limit, the present application This is because it is preferable to use it within the upper and lower limits of the raw material components described in the claims of the present application because the immune enhancing ability to be obtained from is significantly lowered.
  • the present technology forms an autoimmunity enhancer composition of a plant in which aspirin (acetylsalicylic acid, ASA) and benzothiazole are dissolved together in water, and the enhancer composition is applied to the leaves, stems and fruits (fruits) of plants by a conventional spraying method. It is sprayed/provided on the surface and increases the autoimmune function against external environmental stress such as drought, soil salinity, freezing, cold resistance and heat during the plant growth process, ultimately increasing quality and productivity by improving resistance to disease. It can be used in the field to provide.
  • aspirin acetylsalicylic acid, ASA
  • benzothiazole benzothiazole

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present application is an invention relating to a composition for autoimmunity builder of plants in which acetylsalicyclic acid and benzothiazole are dissolved together in water, the composition sprayed on a leaf, stem, and fruit surface of plants according to a general spray method and enhancing functions of enhancing autoimmunity against foreign environment stress on drought, salinity of the soil, freezing, cold resistance, and heat during a process of plant growth. The composition for autoimmunity builder of plants of the present application is a technology provided as a composition for autoimmunity builder of plants which consists of 1.5 to 5.0 wt% of acetylsalicyclic acid (ASA) and 0.5 to 2.0 wt% of benzothiazole dissolved in water, and a preservative dissolved in the amount of 0.05 to 0.25 wt%, a penetrant dissolved in the amount of 0.45 to 3.5 wt%, and an antifoaming agent dissolved in the amount of 0.10 to 0.50 wt%.

Description

식물의 자가면역 증강제 조성물 및 이를 이용한 이용방법Plant autoimmunity enhancer composition and method of use using the same
본 발명은 수중에 아스피린(acetylsalicylic acid, ASA)과 벤조치아졸(Benzothiazole)이 함께 용존된 식물의 자가면역 증강제 조성물을 이루고 증강제 조성물을 통상의 분무방법에 의해 식물의 잎, 줄기 및 열매(과실) 표면에 살포/제공하고, 식물의 성장과정 중 가뭄, 토양의 염분, 동결, 내한성 및 열에 대한 외부의 환경 스트레스에 대해 자가면역의 기능을 증강시켜 궁극적으로 질병에 대한 저항성 향상에 따른 작물의 고품질 및 생산성 증가를 제공할 수 있는 아스피린과 벤조치아졸의 유효성분이 포함된 식물의 자가면역 증강제 조성물에 관한 것이다. The present invention forms an autoimmune enhancing composition of a plant in which aspirin (acetylsalicylic acid, ASA) and benzothiazole are dissolved together in water, and the enhancing agent composition is applied to the leaves, stems and fruits (fruits) of plants by a conventional spraying method. It is sprayed/provided on the surface and enhances the autoimmune function against external environmental stresses such as drought, soil salinity, freezing, cold resistance and heat during the plant growth process, ultimately improving the quality and quality of crops by improving resistance to disease. It relates to a plant autoimmunity enhancer composition containing active ingredients of aspirin and benzozoazole capable of increasing productivity.
식물은 발아에서부터 사멸까지의 생존 기간 동안 주위 환경으로부터 끊임없는 스트레스를 받게 되는데 동물과 달리 식물은 이러한 스트레스를 피해 이동할 수 있는 능력을 보유하고 있지 않으므로, 스트레스에 대해 능동적으로 맞서기 위한 정교한 내부 조절 체계를 확립이 필요하다.Plants are constantly subjected to stress from the surrounding environment during their survival period from germination to death. Unlike animals, plants do not have the ability to move around to avoid such stress, so they have a sophisticated internal control system to actively cope with stress. need to establish
식물이 경험하는 스트레스는 크게 병충해로부터 유래되는 생물학적 스트레스와 환경으로부터 유래되는 비생물학적 스트레스로 나누어질 수 있는데 이 중 비생물학적 스트레스로는 가뭄, 냉해, 고온, 자외선(UV, Ultraviolet) 스트레스 등이 있고, 이들은 식량작물, 에너지작물 등의 생산량을 떨어뜨리는 대표적인 원인으로 지적되어 왔다. Stress experienced by plants can be largely divided into biotic stress derived from pests and abiotic stress derived from the environment. These have been pointed out as a representative cause of reducing the production of food crops and energy crops.
식물의 스트레스 관리는 날로 증가하는 인구와 경작지 축소 등의 문제를 안고 있는 인류에게 매우 절실한 과제다. 하지만 현재 지구는 식물에게 스트레스를 더욱 많이 발생시키는 상황으로 나아가는 원인은 기후변화 때문으로 예측된다.Plant stress management is a very urgent task for mankind, which faces problems such as the ever-increasing population and reduction of arable land. However, it is predicted that climate change is the cause of the present Earth moving toward a situation that causes more stress to plants.
평균기온이 상승하면 호수와 저수지의 증발산 손실량이 증가함으로써 염도가 높아지게 된다. 또 강수량 감소는 많은 지역에서 하천의 유량을 감소시켜 염분 집적량을 더욱 증가시키고, 그 결과 토양의 염류화로 이어진다.As average temperatures rise, evapotranspiration losses in lakes and reservoirs increase, leading to higher salinity. In addition, reduced precipitation reduces river flow in many areas, further increasing salt accumulation, which in turn leads to soil salinization.
현재 전체 식물의 20 %, 그리고 전 세계 경작지의 33%가 이 같은 토양의 고염도화 스트레스를 받고 있다. 기후변화로 인해 전 세계적으로 점점 더 많은 작물이 고염분 스트레스에 직면하게 될 예정이다. Currently, 20% of all plants and 33% of arable land worldwide are under the stress of high soil salinity. Due to climate change, more and more crops worldwide are set to face high salinity stress.
환경이 주는 다양한 스트레스는 식물의 성장, 발달 및 생산성 등에 큰 영향을 주고 있다. 따라서 이러한 환경적인 스트레스에 저항성을 높이는 것은 농업적으로 매우 중요하다.Various stresses given by the environment greatly affect the growth, development, and productivity of plants. Therefore, increasing resistance to these environmental stresses is of great agricultural importance.
따라서 식물 스트레스로 인한 작물 손실량을 단 1%만 줄여도 미래의 식량문제를 획기적으로 개선할 것으로 기대됨에 따라 인간의 스트레스 못지않게 식물의 스트레스 관리가 매우 중요하다.Therefore, as it is expected to drastically improve future food problems even if crop loss due to plant stress is reduced by only 1%, managing plant stress is as important as human stress.
지금까지 환경이 제공하는 다양한 스트레스는 식물의 성장, 발달 및 생산성 등에 큰 영향을 주고 있다. 이에 식물의 스트레스에 대한 대처 방법을 강구하고 있으나 아직까지 이에 대한 효능은 그다지 높지 않은 실정에 있다. Until now, various stresses provided by the environment have a great influence on the growth, development, and productivity of plants. Therefore, a method for coping with the stress of plants is being sought, but the efficacy for this is not yet very high.
종래기술에서 식물의 스트레스 문제를 인식하여 이를 해결을 위해 개시된 수단들을 살펴보면, 한국공개특허 공개번호 10-2021-0085328 A에서는 식물생장촉진세균(Plant-growth-promoting bacteria, PGPR)인 페니바실러스 용인엔시스 DCY84T(Paenibacillus yonginensis DCY84T) 균주를 포함하는 인삼의 염류 스트레스 억제용 조성물을 제시하고 있으며, 한국공개특허 공개번호 10-2021-0069224 A에서는 서열번호 1의 염기서열로 이루어진 BrPP5 유전자를 포함하는 식물의 가뭄 및 고온 스트레스 저항성을 가지는 배추 유래 유전자 및 이의 용도를 제시하고 있고, 한국공개특허 출원번호 10-2019-0133422에서는 식물의 염 스트레스 내성을 조절하는 벼 유래의 OsCP1(Oryza sativa cysteine proteinase 1) 유전자 및 이의 용도를 제시하고 있다.Recognizing the stress problem of plants in the prior art and looking at the means disclosed to solve it, in Korean Patent Publication No. 10-2021-0085328 A, a plant-growth-promoting bacteria (PGPR) Pennybacillus Yongin Nsis A composition for inhibiting salt stress in ginseng containing a DCY84T (Paenibacillus yonginensis DCY84T) strain is proposed, and in Korea Patent Publication No. 10-2021-0069224 A, drought in plants containing the BrPP5 gene consisting of the nucleotide sequence of SEQ ID NO: 1 And a Chinese cabbage-derived gene having high temperature stress resistance and its use are proposed, and in Korea Patent Publication No. 10-2019-0133422, rice-derived OsCP1 (Oryza sativa cysteine proteinase 1) gene and its use is presented.
또한 미국공개특허 출원번호 16/267910에서는 농업용 작물에 발아의 자극을 주기 위하여 식물 성장과 저항을 위한 조성물을 제시하고 있고 미국공개특허 공개번호 2021-0180074 A1에서는 비생물적 스트레스 내성 식물과 비생물적 스트레스와 방법을 개선하기 위해 폴리뉴클레오티드를 제시하고 있으며, 미국공개특허 공개번호 2020-0095595 A1에서는 비생물적 스트레스 조건하에서 다수확 작물을 생산하는 방법을 제시하고 있고 미국공개특허 공개번호 2020-0208053 A1에서는 식물에 물 스트레스를 줄일 수 있는 방법을 제시하고 있다.In addition, US Patent Publication No. 16/267910 proposes a composition for plant growth and resistance in order to stimulate germination in agricultural crops, and US Patent Publication No. 2021-0180074 A1 discloses abiotic stress-resistant plants and abiotic Polynucleotides are proposed to improve stress and methods, and US Patent Publication No. 2020-0095595 A1 suggests a method for producing high-yield crops under abiotic stress conditions, and US Patent Publication No. 2020-0208053 A1 Suggests ways to reduce water stress on plants.
일본공개특허 공개번호 2021-093933 A에서는 개화 예정일에 활성 산소를 식물에 접촉하는 방법에 의해 식물에 환경 스트레스 내성을 부여하는 방법을 제시하고 있고, 일본공개특허 공개번호 2018-525440에서는 아미드7-카르복시 벤조[1,2,3]티아디아졸 및 그 유도체가 포함된 식물 자극제에 의한 식물의 성장을 조절하거나, 식물의 대사 프로세스를 조절하거나, 식물의 생리적 프로세스를 조절하거나, 식물의 생물 스트레스의 영향을 예방하거나, 또는 식물의 비생물 스트레스의 효과를 예방할 수 있는 적용방법을 제시하고 있으며, 일본공개특허 공개번호 2018-511562에서는 식물 생장 조절제로서의 2-옥소-3,4-디하이드로퀴놀린 화합물을 제시하고 있으며, 일본공개특허 공개번호 2017-137281에서는 알란토인을 유효성분으로서 함유하는 식물의 고온 스트레스 내성 향상제, 고온 스트레스 내성을 향상시키는 방법, 백화 억제제 및 DREB2A 유전자 발현 촉진제 등이 제시되어 있다.Japanese Unexamined Patent Publication No. 2021-093933 A proposes a method for imparting environmental stress resistance to plants by contacting active oxygen to plants on the expected date of flowering, and Japanese Unexamined Patent Publication No. 2018-525440 discloses amide 7-carboxy Regulating plant growth, regulating plant metabolic processes, regulating plant physiological processes, or affecting plant biotic stress by plant stimulants including benzo[1,2,3]thiadiazoles and their derivatives It suggests an application method that can prevent or prevent the effects of abiotic stress on plants, and Japanese Patent Publication No. 2018-511562 suggests a 2-oxo-3,4-dihydroquinoline compound as a plant growth regulator. And, Japanese Patent Laid-open Publication No. 2017-137281 suggests a high temperature stress tolerance improver of plants containing allantoin as an active ingredient, a method for improving high temperature stress tolerance, a bleaching inhibitor and a DREB2A gene expression promoter.
그러나 상기 제시한 종래기술에서는 본원의 기술사상과 같이 아스피린과 벤조치아졸을 유효성분으로 하는 식물의 자가면역 증강제에 관한 기술사상과 유사한 기술사상은 선행기술에서 제시된 바 없으며, 또한 선행기술들이 복합되어 적용되는 경우에도 본원 기술이 제공하고자 하는 목적이나 효과을 달성할 수 없는 기술임을 확인하여 특허출원에 이르게 되었다.However, in the prior art presented above, as in the technical idea of the present application, a technical idea similar to that of a plant autoimmune enhancer containing aspirin and benzozoazole as active ingredients has not been presented in the prior art, and also the prior art is combined Even when applied, it was confirmed that the technology of the present application was a technology that could not achieve the purpose or effect to be provided, leading to a patent application.
본원은 수중에 아스피린(acetylsalicylic acid, ASA)과 벤조치아졸(Benzothiazole)이 함께 용존된 식물의 자가면역 증강제 조성물을 분무방법에 의해 식물의 잎, 줄기 및 열매(과실) 표면에 제공하고, 식물의 성장과정 중 가뭄, 토양의 염분, 동결, 내한성 및 열에 대한 외부의 환경 스트레스에 대해 자가면역의 기능을 증강시켜 감염 억제 및 질병에 대한 저항성 향상에 따른 작물의 고품질 및 생산성 증가를 제공할 수 있는 아스피린과 벤조치아졸의 유효성분이 포함된 식물의 자가면역 증강제 조성물 및 이의 이용방법을 제공하기 위한 발명의 목적을 갖는다.The present application provides a plant autoimmunity enhancing composition in which aspirin (acetylsalicylic acid, ASA) and benzothiazole are dissolved together in water to the leaves, stems and fruit (fruit) surfaces of plants by spraying, Aspirin enhances the autoimmune function against external environmental stress such as drought, soil salinity, freezing, cold resistance and heat during the growth process, thereby suppressing infection and improving resistance to disease, thereby providing high quality and increased productivity of crops. It is an object of the invention to provide a plant autoimmunity enhancing composition containing active ingredients of and benzozoazole and a method of using the same.
본원 기술은 상기 목적을 달성하기 위한 수단으로서, 즉 작물 재배과정 중 외부 환경으로부터 제공되는 스트레스를 해소하기 위한 식물의 자가면역 증강제 조성물로, 수중에 아스피린(acetylsalicylic acid, ASA)이 1.5 내지는 5.0 중량%와 벤조치아졸(Benzothiazole)이 0.5 내지는 2.0 중량%이 용존되고, 방부제가 0.05 내지는 0.25 중량%와 침투제가 0.45 내지는 3.5 중량%와 소포제가 0.10 내지는 0.50 중량 %로 용해되어 이루어지는 식물의 자가면역 증강제 조성물을 이루도록 제공된다.The technology of the present invention is a plant autoimmunity enhancer composition as a means to achieve the above object, that is, to relieve the stress provided from the external environment during the crop cultivation process, in which 1.5 to 5.0% by weight of aspirin (acetylsalicylic acid, ASA) is contained in water. and 0.5 to 2.0% by weight of benzothiazole, 0.05 to 0.25% by weight of preservative, 0.45 to 3.5% by weight of penetrant, and 0.10 to 0.50% by weight of antifoaming agent. provided to achieve
본원에서 제공되는 자가면역 증강제 조성물에서 상기 방부제는 소르빈산, 안식향산, 파라옥시안식향산, 프로피온산, 메칠파라벤, 프로필파라벤, 페녹시에탄올, 디아졸리디닐우레아, 이미다졸리디닐우레아 중 1종 이상의 방부제가 선택되어 사용될 수 있고, 상기 침투제는 나프탈렌술폰산나트륨-포르말린 축합물, 알킬나프탈렌술폰산나트륨 또는 그의 포르말린축합물, 페놀술폰산나트륨과 나프톨술폰산나트륨의 포르말린축합물, 알킬 디페닐 에테르 디술폰산 나트륨, 리그닌술폰산 나트륨, 비이온계 계면활성제 폴리옥시에틸렌 알킬(알릴)에테르, 폴리아크릴산나트륨, 올레핀-말레인산나트륨 공중합체, 축합인산염, 알킬벤젠(나프탈렌)술폰산염, 디옥틸술폰말레인산나트륨, 스티렌-무수말레인산 공중합체의 부분아미드화/부분에스테르화물 중 1종 이상의 침투제가 선택되어 사용될 수 있으며, 상기 소포제는 디메틸폴리실록산 내지는 메틸페닐폴리실록산 내지는 메틸비닐폴리실록산 등의 직쇄형 실리콘 오일이 주성분으로 이루어지는 실리콘계 소포제가 사용될 수 있다.In the autoimmunity enhancing composition provided herein, the preservative is at least one preservative selected from sorbic acid, benzoic acid, paraoxybenzoic acid, propionic acid, methylparaben, propylparaben, phenoxyethanol, diazolidinyl urea, and imidazolidinyl urea. The penetrant may be sodium naphthalenesulfonate-formalin condensate, sodium alkylnaphthalenesulfonate or its formalin condensate, formalin condensate of sodium phenolsulfonate and sodium naphtholsulfonate, sodium alkyl diphenyl ether disulfonate, sodium ligninsulfonate, b Ionic surfactant Polyoxyethylene alkyl (allyl) ether, sodium polyacrylate, olefin-sodium maleate copolymer, condensed phosphate, alkylbenzene (naphthalene) sulfonate, dioctylsulfone maleate sodium, styrene-maleic anhydride copolymer partial amide One or more types of penetrant may be selected and used among esterification/partial esterification, and the antifoaming agent may be a silicone-based antifoaming agent composed mainly of linear silicone oil such as dimethylpolysiloxane, methylphenylpolysiloxane, or methylvinylpolysiloxane.
본원은 작물 재배과정 중 외부 환경으로부터 제공되는 스트레스를 해소하기 위한 식물의 자가면역 증강방법으로서, 수중에 아스피린(acetylsalicylic acid, ASA)이 1.5 내지는 5.0 중량%와 벤조치아졸(Benzothiazole)이 0.5 내지는 2.0 중량%이 용존되고, 방부제가 0.05 내지는 0.25 중량%와 침투제가 0.45 내지는 3.5 중량%와 소포제가 0.10 내지는 0.50 중량 %로 용해되어 이루어지는 식물의 자가면역 증강제 조성물을 이루고, 상기 자가면역 증강제 조성물을 1/150 내지는 1/1,500의 범위로 물에 희석 후 수동 및 자동화 분무장비를 동원하여 식물의 잎, 줄기, 열매에 분무시켜 식물의 자가면역력을 높이도록 적용되는 것을 특징으로 하는 식물의 자가면역 증강방법이 개시된다. The present application is a method for enhancing autoimmunity of plants to relieve stress provided from the external environment during the crop cultivation process, by adding 1.5 to 5.0% by weight of aspirin (acetylsalicylic acid, ASA) and 0.5 to 2.0% by weight of benzozoazole in water. 0.05 to 0.25% by weight of the preservative, 0.45 to 3.5% by weight of the penetrant, and 0.10 to 0.50% by weight of the antifoaming agent are dissolved to form a plant autoimmunity enhancing composition, wherein the autoimmune enhancing agent composition is 1/ After diluting in water in the range of 150 to 1/1,500, manual and automated spraying equipment is used to spray the leaves, stems, and fruits of plants to enhance plant autoimmunity. is initiated
본원에서 아스피린(acetylsalicylic acid, ASA)은 외부의 환경 스트레스에 대한 자가면역 기능을 제공하는데 목적을 두고 있으며, 살리실산과 아세트산을 혼합한 후 산 촉매하에 에스터화에 의해 만들어 사용할 수 있으며, 진통제, 해열제로 사용하고 있는 아스피린 의약품을 구입하여 사용할 수 있으나, 광범위한 식물의 자가면역 분야에 사용되어 많은 량이 필요하기 때문에 가능한 제조해서 사용하는 것이 바람직하다.Here, aspirin (acetylsalicylic acid, ASA) aims to provide autoimmune function against external environmental stress. It can be used by mixing salicylic acid and acetic acid and then esterifying it under an acid catalyst. It is possible to purchase and use an aspirin drug that is being used, but it is preferable to manufacture and use it as much as possible because it is used in a wide range of plant autoimmune fields and requires a large amount.
또한 상기 벤조치아졸(Benzothiazole)은 외부의 환경 스트레스에 대한 자가면역 기능을 제공하기 위한 아스피린의 자가면역 기능을 배가시킴은 물론 다양한 외부환경에 대한 스트레스의 자가면역 기능을 활성화시키는데 목적을 두고 있으며, 특별한 제한없이 판매되는 약품을 구입하여 사용하는 것이 바람직하다. In addition, the benzothiazole is aimed at doubling the autoimmune function of aspirin to provide autoimmune function against external environmental stress, as well as activating the autoimmune function of stress against various external environments, It is desirable to purchase and use commercially available drugs without particular restrictions.
상기 방부제는 아스피린과 벤조치아졸이 포함된 식물의 자가면역 증강제 조성물에 포함된 유기물들이 적당한 온도와 기간의 경과에 따라 미생물이나 곰팡이의 발생으로 인하여 보존성을 비롯한 조성물의 기능 및 상품성이 떨어짐을 방지하기 위한 목적으로 사용되며, 상기 침투제는 식물의 줄기, 잎, 열매(과실)에 분무된 아스피린과 벤조치아졸의 자가면역 증강제 유효성분이 식물의 줄기, 잎, 열매(과실) 표피 조직 내부로 침투율을 증강시켜 자가면역 증강제의 효과를 높여주기 위한 목적으로 사용되고 상기 소포제는 본원의 기술구성에 포함되는 침투제로 인하여 본원의 자가면역 증강제 조성물이 다량의 거품이 발생함에 따라 제조과정 및 분무 과정에서 발생되는 문제를 방지하기 위한 목적을 두고 사용된다.The preservative is to prevent deterioration in function and marketability of the composition, including preservation due to the occurrence of microorganisms or molds when the organic substances contained in the plant's autoimmune enhancing composition containing aspirin and benzozoazole are passed at an appropriate temperature and over a period of time. The penetrant is used for the purpose of enhancing the penetration rate of the autoimmune enhancer active ingredients of aspirin and benzoxazole sprayed on the stem, leaf, and fruit (fruit) of the plant into the epidermal tissue of the plant stem, leaf, and fruit (fruit). The antifoaming agent is used for the purpose of enhancing the effect of the autoimmune enhancer, and the antifoaming agent causes the autoimmune enhancer composition of the present application to generate a large amount of foam due to the penetrant included in the technical configuration of the present application. It is used for the purpose of preventing
따라서 본 발명은 아스피린과 벤조치아졸의 유효성분이 포함된 식물의 자가면역 증강제 조성물에 의해 식물의 성장과정 중 가뭄, 토양의 염분, 동결, 내한성 및 열에 대한 외부의 환경 스트레스에 대해 자가면역의 기능을 증강시켜 감염 억제 및 질병에 대한 저항성 향상에 따른 작물의 고품질 및 생산성 증가를 제공할 수 있는 식물의 자가면역 증강제 조성물 및 이를 이용한 이용방법에 의해 더욱 광범위 해지는 외부 환경스트레스 충분히 극복할 수 있어 모든 농작물을 재배하는 농가에 널리 적용될 수 있을 것으로 기대된다.Therefore, the present invention is a plant autoimmunity enhancer composition containing active ingredients of aspirin and benzoxazole to improve the autoimmune function against external environmental stresses such as drought, soil salinity, freezing, cold tolerance and heat during plant growth. An autoimmune enhancer composition of plants capable of providing high quality and increased productivity of crops by suppressing infection and improving resistance to diseases by enhancing plant autoimmunity and a method of using the same can sufficiently overcome a wider range of external environmental stress, so that all crops can be It is expected to be widely applied to farming households.
종래의 기술에서는 스트레스와 관련된 자가면역 효율이 높지 않아 지금까지 환경이 제공하는 다양한 스트레스 때문에 식물의 성장, 발달 및 생산성 등에 큰 영향에 따른 수확량이 낮게 생산되어 미래의 식량문제를 충분히 해결할 수 없다는 문제점이 있다. In the prior art, the efficiency of autoimmunity related to stress is not high, so far, due to the various stresses provided by the environment, the yield due to the great influence on the growth, development, and productivity of plants is produced low, so that future food problems cannot be sufficiently solved. there is.
그러나 본원의 식물의 자가면역 증강제에 의해 외부환경 변화에 따른 식물이 접하는 다양한 스트레스의 문제점을 충분히 해결할 수 있어, 고품질의 작물을 다수확할 수 있어, 미래의 식량문제제를 크게 해결할 수 있음은 물론 품질 향상에 의한 고품질의 상품성을 유지할 수 있어 농가의 생산활동을 위한 경제적 소득에 이바지할 수 있는 효과로 인해 농가소득증대에 크게 기여할 것으로 기대된다.However, the plant autoimmunity enhancer of the present application can sufficiently solve the problems of various stresses encountered by plants due to changes in the external environment, and can produce a large number of high-quality crops, thereby greatly solving future food problems as well as quality It is expected that it will greatly contribute to the increase in farm household income due to the effect that can contribute to economic income for farm household production activities by maintaining high quality marketability by improvement.
본원 기술은 작물 재배과정 중 외부 환경으로부터 제공되는 스트레스를 해소하기 위한 식물의 자가면역 증강제 조성물을 제공하기 위한 기술로써, 수중에 아스피린(acetylsalicylic acid, ASA)이 1.5 내지는 5.0 중량%와 벤조치아졸(Benzothiazole)이 0.5 내지는 2.0 중량%이 용존되고, 방부제가 0.05 내지는 0.25 중량%와 침투제가 0.45 내지는 3.5 중량%와 소포제가 0.10 내지는 0.50 중량 %로 용해되어 이루어지는 식물의 자가면역 증강제 조성물을 이루고 외부환경 변화에 따라 식물이 접하는 다양한 스트레스의 문제점을 해결하고 고품질의 작물을 다수확할 수 있는 식물의 자가면역 증강제 조성물을 얻기 위한 최선의 실시형태는 식물 자가 면역증강제의 유효성분으로 아스피린이 1.5 ㎏, 벤조치아졸(삼전순약) 0.05 ㎏과 방부제로 안식향산(삼전순약) 0.05 ㎏, 침투제로 알킬 디페닐 에테르 디술폰산 나트륨[㈜미원] 0.5 ㎏, 소포제로 디메틸폴리실록산[㈜블루마운트테크놀러지] 0.1 ㎏으로 이루어지는 식물의 자가면역 증가제 조성물을 제조한 후 물을 이용하여 1/200으로 희석하고, 660 ㎡ 비닐하우스에서 재배 중인 곡물이나 과일의 잎, 줄기, 과실에 스프링 클러를 이용하여 살포하는 수단으로 본원 발명의 목적을 달성할 수 있다.The technology of the present invention is a technology for providing a plant autoimmune enhancing agent composition for relieving stress provided from the external environment during the crop cultivation process, in which aspirin (acetylsalicylic acid, ASA) is 1.5 to 5.0% by weight and benzozoazole ( Benzothiazole) is dissolved in 0.5 to 2.0% by weight, preservative is 0.05 to 0.25% by weight, penetrant is dissolved in 0.45 to 3.5% by weight, and antifoaming agent is dissolved in 0.10 to 0.50% by weight to form an autoimmune enhancing composition for plants and changes in the external environment. According to the best embodiment for obtaining a plant autoimmunity enhancer composition capable of solving the problems of various stresses encountered by plants and yielding high-quality crops, 1.5 kg of aspirin and benzozoazole as active ingredients of the plant autoimmunity enhancer (Samjeon Pure Medicine) 0.05 kg, benzoic acid (Samjeon Pure Medicine) 0.05 kg as a preservative, alkyl diphenyl ether disulfonate sodium [Miwon Co., Ltd.] 0.5 kg as a penetrating agent, and dimethylpolysiloxane [Bluemount Technology Co., Ltd.] 0.1 kg as an antifoaming agent. The object of the present invention is a means of preparing an immune booster composition, diluting it with water to 1/200, and spraying the leaves, stems, and fruits of grains or fruits grown in a 660 m2 greenhouse using a sprinkler. can be achieved
본원의 기술사상을 구현하기 위한 발명의 실시내용을 실시예로 기재하기에 앞서, 본 출원의 명세서나 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니 될 것이며, 본원의 보호범위는 본원발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 할 것이며, 본 명세서에 기재된 예시는 본 발명의 가장 바람직한 일 실시 예에 불과할 뿐이고 본원의 기술사상을 모두 대변하는 것은 아니므로, 본 출원시점에 있어서 이들을 대체할 수 있는 다양한 균등물과 변형 예들이 있을 수 있음을 이해하여야 할 것이다.Prior to describing the embodiments of the invention for implementing the technical idea of the present application as examples, the terms or words used in the specification or claims of the present application should not be construed as being limited to a common or dictionary meaning, The protection scope of the present application should be interpreted as meaning and concept consistent with the technical spirit of the present invention, and the examples described in this specification are only one of the most preferred embodiments of the present invention and do not represent all of the technical spirit of the present invention. , It should be understood that there may be various equivalents and modifications that can replace them at the time of this application.
이하, 본 발명을 다음의 실시 예에 의하여 본원의 기술사상이 적용되는 예를 상세하게 설명하고자 한다.Hereinafter, an example to which the technical idea of the present application is applied will be described in detail by the following embodiments of the present invention.
살리실산(Salicylic acid, 삼전순약)과 빙초산을 혼합하고, 인산의 촉매하에 합성한 후 2 oC의 냉장고에 하루 방치하여 아스피린 석출물을 수득한 후 아래의 실시 예에 따른 아스피린 공급원으로 하였다. Salicylic acid (Samjeon Pure Medicine) and glacial acetic acid were mixed, synthesized under the catalyst of phosphoric acid, and left in a refrigerator at 2 o C for one day to obtain an aspirin precipitate, which was then used as an aspirin source according to the following examples.
실시 예 1Example 1
식물 자가 면역증강제의 유효성분으로 아스피린이 1.5 ㎏, 벤조치아졸(삼전순약) 0.05 ㎏과 방부제로 안식향산(삼전순약) 0.05 ㎏, 침투제로 알킬 디페닐 에테르 디술폰산 나트륨[㈜미원] 0.5 ㎏, 소포제로 디메틸폴리실록산[㈜블루마운트테크놀러지] 0.1 ㎏으로 이루어지는 식물의 자가면역 증가제 조성물을 제조한 후 1/200으로 희석하고, 660 ㎡ 비닐하우스에서 재배 중인 450포기 수박의 잎, 줄기, 과실에 스프링클러를 이용하여 120일 동안 1회/2일 살포하고, 450포기 수박의 평균 무게를 확인하였다. 1.5 kg of aspirin, 0.05 kg of benzozoazole (Samjeon Pure Medicine) and 0.05 kg of benzoic acid (Samjeon Pure Medicine) as a preservative, 0.5 kg of sodium alkyl diphenyl ether disulfonate [Miwon Co., Ltd.] as a penetrant, antifoaming agent After preparing a plant autoimmune booster composition consisting of 0.1 kg of dimethylpolysiloxane [Bluemount Technology Co., Ltd.], it was diluted to 1/200, and sprinklers were applied to the leaves, stems, and fruits of 450 watermelons grown in a 660 m2 vinyl house. It was sprayed once/two days for 120 days, and the average weight of 450 heads of watermelon was confirmed.
실시 예 2Example 2
식물 자가 면역증강제의 유효성분으로 아스피린이 3.0 ㎏, 벤조치아졸(삼전순약) 0.15 ㎏과 방부제로 메칠파라베(삼전순약) 0.10 ㎏, 침투제로 폴리아크릴산나트륨[㈜한농화성] 2.0 ㎏, 소포제로 디메틸폴리실록산[㈜블루마운트테크놀러지] 0.25 ㎏으로 이루어지는 식물의 자가면역 증가제 조성물을 제조한 후 1/750으로 희석하고, 660 ㎡ 비닐하우스에서 재배 중인 약 8,000포기 배추에 스프링클러를 이용하여 100일 동안 1회/2일 살포하고, 8,000 포기 배추의 평균 무게를 확인하였다. 3.0 kg of aspirin, 0.15 kg of benzozoazole (Samjeon Pure Medicine), 0.10 kg of methyl parabe (Samjeon Pure Medicine) as a preservative, 2.0 kg of sodium polyacrylate [Han Nong Chemical Co., Ltd.] as a penetrant, and 2.0 kg of sodium polyacrylate as an antifoaming agent Dimethylpolysiloxane [Bluemount Technology Co., Ltd.] After preparing a plant autoimmune booster composition consisting of 0.25 kg, diluting it to 1/750, using a sprinkler for about 8,000 heads of cabbage grown in a 660 ㎡ vinyl house, 1 for 100 days It was sprayed once/2 days, and the average weight of 8,000 heads of cabbage was confirmed.
실시 예 3Example 3
식물 자가 면역증강제의 유효성분으로 아스피린이 5.0 ㎏, 벤조치아졸(삼전순약) 0.25 ㎏과 방부제로 페녹시에탄올(삼전순약) 0.25 ㎏, 침투제로 올레핀-말레인산나트륨 공중합체[㈜애경화학] 3.5 ㎏, 소포제로 디메틸폴리실록산[㈜블루마운트테크놀러지] 0.5 ㎏으로 이루어지는 식물의 자가면역 증가제 조성물을 제조한 후 1/1,500으로 희석하고, 660 ㎡ 비닐하우스에서 재배 중인 캠밸 품종 포도 180주에 대한 포도 한송이의 평균 무게를 확인하였다.5.0 kg of aspirin, 0.25 kg of benzozoazole (Samjeon Pure Medicine) as active ingredients of the plant autoimmune enhancer, 0.25 kg of phenoxyethanol (Samjeon Pure Medicine) as a preservative, and 3.5 kg of olefin-sodium maleate copolymer [Aekyung Chemical Co., Ltd.] as a penetrant After preparing a plant autoimmune booster composition consisting of 0.5 kg of dimethylpolysiloxane [Bluemount Technology Co., Ltd.] as an antifoaming agent, diluting it by 1/1,500, one grape for 180 Campbell grapes grown in a 660 m2 vinyl house The average weight was confirmed.
비교 예 1~3Comparative Examples 1 to 3
식물 자가 면역증강제 조성물 대신에 순수한 수돗물을 이용하여 실시 예 1~3과 동일한 방법으로 수행하였다. It was performed in the same manner as in Examples 1 to 3 using pure tap water instead of the plant autoimmune adjuvant composition.
비교 예 1~3 및 실시 예 1~3의 결과를 표 1에 나타냈다.Table 1 shows the results of Comparative Examples 1 to 3 and Examples 1 to 3.
구분division 수확 작물에 대한 평균 무게Average Weight for Harvested Crops
수박(kg/개)Watermelon (kg/piece) 배추(kg/포기)Cabbage (kg/abandon) 포도(g/한송이)Grapes (g/one bunch)
실시 예 1Example 1 9.839.83 -- --
실시 예 2Example 2 -- 5.115.11 --
실시 예 3Example 3 -- -- 336336
비교 예 1Comparative Example 1 7.167.16 -- --
비교 예 2Comparative Example 2 -- 3.523.52 --
비교 예 3Comparative Example 3 -- -- 284284
상기 표 1에서 나타낸 바와 같이 식물 자가 면역증강제 조성물을 식물의 잎, 줄기, 과실에 제공하지 않고, 순수한 수돗물을 작물에 공급한 비교 예 1~3에서는 수박의 평균 무게가 7.16 kg을 나타냈으며, 배추의 경우에는 포기당 3.52 kg의 평균무게와 포도의 경우에는 한 송이당 284 g의 평균 무게를 가진 작물을 수확할 ㅅ수 있었다.As shown in Table 1, in Comparative Examples 1 to 3 in which pure tap water was supplied to the crops without the plant autoimmune enhancer composition being applied to the leaves, stems, and fruits of the plant, the average weight of watermelon was 7.16 kg, and Chinese cabbage It was possible to harvest crops with an average weight of 3.52 kg per plant in the case of grapes and 284 g per bunch in the case of grapes.
반면, 실시 예 1~3에서는 식물 자가 면역증강제 조성물을 수박, 배추 및 포도의 작물에 분무, 코팅하였을 경우 수박은 평균 무게가 9.83 kg을 나타냈으며, 배추의 경우에는 포기당 5.11 kg을 나타냈고, 포도인 경우에는 송이당 336 g을 나타낸 바, 본원의 식물 자가 면역증강제의 유효성분인 아스피린과 벤조치아졸이 함께 구성된 조성물이 작물 재배 과정 중 식물의 잎, 줄기, 과실에 코팅되어 구성될 경우 외부 환경에 대한 스트레스를 해결하여 품질이 최상위인 농작물을 재배할 수 있음을 확인하였다. On the other hand, in Examples 1 to 3, when the plant autoimmune enhancer composition was sprayed and coated on crops of watermelon, Chinese cabbage and grapes, the watermelon showed an average weight of 9.83 kg, and in the case of Chinese cabbage, the average weight was 5.11 kg per bag, In the case of grapes, 336 g per bunch is shown. When the composition composed of aspirin and benzozoazole, the active ingredients of the plant autoimmune enhancer of the present application, is coated on the leaves, stems, and fruits of plants during the crop cultivation process, the outside It was confirmed that it was possible to grow crops of the highest quality by solving the stress on the environment.
따라서 본원의 기술구성에 의해 종래 작물 재배 과정 중 발생하는 외부환경에 대한 다양한 스트레스로 인하여 상품성이 크게 떨어지고, 수확량이 낮아져 농가의 커다란 경제적 손실을 포함한 미래의 식량문제를 충분히 극복할 수 있을 것으로 기대된다. Therefore, it is expected that the technical configuration of the present application will be able to sufficiently overcome future food problems, including large economic losses of farmers, due to a significant drop in marketability and low yield due to various stresses on the external environment occurring during the conventional crop cultivation process. .
본원의 청구범위에 기재된 원료성분의 상한 및 하한의 한계가 설정된 이유는 상한을 초과하는 경우 면역증강제 조성물의 제조원가가 높아지는 것에 비해 조성물이 제공하는 효과가 미미하기 때문이며, 하한의 범위 이하로 적용될 때는 본원에서 얻고자 하는 면역증강능력이 현저히 낮아지기 때문에 본원의 청구범위에 기재된 원료성분의 상한 및 하한 범위로 사용됨이 바람직하기 때문이다.The reason why the upper and lower limits of the raw material components described in the claims of the present application are set is that the effect provided by the composition is insignificant compared to the increase in the manufacturing cost of the immune enhancer composition when the upper limit is exceeded, and when applied below the lower limit, the present application This is because it is preferable to use it within the upper and lower limits of the raw material components described in the claims of the present application because the immune enhancing ability to be obtained from is significantly lowered.
본원기술은 수중에 아스피린(acetylsalicylic acid, ASA)과 벤조치아졸(Benzothiazole)이 함께 용존된 식물의 자가면역 증강제 조성물을 이루고 증강제 조성물을 통상의 분무방법에 의해 식물의 잎, 줄기 및 열매(과실) 표면에 살포/제공하고, 식물의 성장과정 중 가뭄, 토양의 염분, 동결, 내한성 및 열에 대한 외부의 환경 스트레스에 대한 자가면역의 기능을 증가시켜 궁극적으로 질병에 대한 저항성 향상에 따른 고품질 및 생산성 증가를 제공하기 위한 분야에 사용될 수 있다. The present technology forms an autoimmunity enhancer composition of a plant in which aspirin (acetylsalicylic acid, ASA) and benzothiazole are dissolved together in water, and the enhancer composition is applied to the leaves, stems and fruits (fruits) of plants by a conventional spraying method. It is sprayed/provided on the surface and increases the autoimmune function against external environmental stress such as drought, soil salinity, freezing, cold resistance and heat during the plant growth process, ultimately increasing quality and productivity by improving resistance to disease. It can be used in the field to provide.

Claims (5)

  1. 작물 재배과정 중 외부 환경으로부터 제공되는 스트레스를 해소하기 위한 식물의 자가면역 증강제 조성물에 있어서,In the plant autoimmunity enhancer composition for relieving stress provided from the external environment during crop cultivation,
    수중에 아스피린(acetylsalicylic acid, ASA)이 1.5 내지는 5.0 중량%와 벤조치아졸(Benzothiazole)이 0.5 내지는 2.0 중량%이 용존되고, 1.5 to 5.0% by weight of aspirin (acetylsalicylic acid, ASA) and 0.5 to 2.0% by weight of benzothiazole are dissolved in water,
    방부제가 0.05 내지는 0.25 중량%와 0.05 to 0.25% by weight of preservatives and
    침투제가 0.45 내지는 3.5 중량%와0.45 to 3.5% by weight of penetrant and
    소포제가 0.10 내지는 0.50 중량 %로 용해되어 이루어지는 식물의 자가면역 증강제 조성물을 이루도록 제공되는 것을 특징으로 하는 식물의 자가면역 증강제 조성물.A plant autoimmunity enhancing composition characterized in that it is provided to form a plant autoimmunity enhancing composition comprising an antifoam dissolved in an amount of 0.10 to 0.50% by weight.
  2. 제1항에 있어서,According to claim 1,
    상기 방부제는 소르빈산, 안식향산, 파라옥시안식향산, 프로피온산, 메칠파라벤, 프로필파라벤, 페녹시에탄올, 디아졸리디닐우레아, 이미다졸리디닐우레아 중 1종 이상의 방부제가 선택되어 사용되는 것을 특징으로 하는 식물의 자가면역 증강제 조성물The preservative is a plant self, characterized in that one or more preservatives selected from among sorbic acid, benzoic acid, paraoxybenzoic acid, propionic acid, methylparaben, propylparaben, phenoxyethanol, diazolidinyl urea, and imidazolidinyl urea are used. immune enhancer composition
  3. 제1항에 있어서,According to claim 1,
    상기 침투제는 나프탈렌술폰산나트륨-포르말린 축합물, 알킬나프탈렌술폰산나트륨 또는 그의 포르말린축합물, 페놀술폰산나트륨과 나프톨술폰산나트륨의 포르말린축합물, 알킬 디페닐 에테르 디술폰산 나트륨, 리그닌술폰산 나트륨, 비이온계 계면활성제 폴리옥시에틸렌 알킬(알릴)에테르, 폴리아크릴산나트륨, 올레핀-말레인산나트륨 공중합체, 축합인산염, 알킬벤젠(나프탈렌)술폰산염, 디옥틸술폰말레인산나트륨, 스티렌-무수말레인산 공중합체의 부분아미드화/부분에스테르화물 중 1종 이상의 침투제가 선택되어 사용되는 것을 특징으로 하는 식물의 자가면역 증강제 조성물The penetrant is sodium naphthalenesulfonate-formalin condensate, sodium alkylnaphthalenesulfonate or its formalin condensate, formalin condensate of sodium phenolsulfonate and sodium naphtholsulfonate, sodium alkyl diphenyl ether disulfonate, sodium ligninsulfonate, nonionic surfactant Polyoxyethylene alkyl (allyl) ether, sodium polyacrylate, olefin-sodium maleate copolymer, condensed phosphate, alkylbenzene (naphthalene) sulfonate, dioctylsulfone sodium maleate, partial amidation/partial ester of styrene-maleic anhydride copolymer Plant autoimmunity enhancing composition, characterized in that one or more penetrants are selected and used in the cargo
  4. 제1항에 있어서,According to claim 1,
    상기 소포제는 디메틸폴리실록산 내지는 메틸페닐폴리실록산 내지는 메틸비닐폴리실록산 등의 직쇄형 실리콘 오일이 주성분으로 이루어지는 실리콘계 소포제가 사용되는 것을 특징으로 하는 선택되어 사용되는 것을 특징으로 하는 식물의 자가면역 증강제 조성물The antifoaming agent is a plant autoimmunity enhancer composition, characterized in that it is selected and used, characterized in that a silicone-based antifoaming agent composed of a linear silicone oil such as dimethylpolysiloxane, methylphenylpolysiloxane or methylvinylpolysiloxane as a main component is used
  5. 작물 재배과정 중 외부 환경으로부터 제공되는 스트레스를 해소하기 위한 식물의 자가면역 증강방법에 있어서,In the plant autoimmune enhancement method for relieving stress provided from the external environment during crop cultivation,
    수중에 아스피린(acetylsalicylic acid, ASA)이 1.5 내지는 5.0 중량%와 벤조치아졸(Benzothiazole)이 0.5 내지는 2.0 중량%이 용존되고, 1.5 to 5.0% by weight of aspirin (acetylsalicylic acid, ASA) and 0.5 to 2.0% by weight of benzothiazole are dissolved in water,
    방부제가 0.05 내지는 0.25 중량%와 0.05 to 0.25% by weight of preservatives and
    침투제가 0.45 내지는 3.5 중량%와0.45 to 3.5% by weight of penetrant and
    소포제가 0.10 내지는 0.50 중량 %로 용해되어 이루어지는 식물의 자가면역 증강제 조성물을 이루고An antifoaming agent is dissolved in an amount of 0.10 to 0.50% by weight to form a plant autoimmunity enhancing composition.
    상기 자가면역 증강제 조성물을 1/150 내지는 1/1,500의 범위로 물에 희석 후 수동 및 자동화 분무장비를 동원하여 식물의 잎, 줄기, 열매에 분무시켜 식물의 자가면역력을 높이도록 적용되는 것을 특징으로 하는 식물의 자가면역 증강방법.After diluting the autoimmune enhancing agent composition in water in the range of 1/150 to 1/1,500, manual and automated spraying equipment is used to spray the leaves, stems, and fruits of plants to increase autoimmunity of plants. A method for enhancing plant autoimmunity.
PCT/KR2022/010742 2021-07-22 2022-07-21 Composition for autoimmune builder of plants and usage method using same WO2023003410A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2021-0096230 2021-07-22
KR1020210096230A KR102431000B1 (en) 2021-07-22 2021-07-22 Composition for an autoimmune builder of plants containing aspirin and benzothiazole, and method of using the same

Publications (1)

Publication Number Publication Date
WO2023003410A1 true WO2023003410A1 (en) 2023-01-26

Family

ID=82803468

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2022/010742 WO2023003410A1 (en) 2021-07-22 2022-07-21 Composition for autoimmune builder of plants and usage method using same

Country Status (2)

Country Link
KR (1) KR102431000B1 (en)
WO (1) WO2023003410A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116515331A (en) * 2023-05-27 2023-08-01 烟台凯多海洋生物研究院有限公司 Preparation and application methods of apple bagging-free film agent

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013535506A (en) * 2010-08-12 2013-09-12 マイクロビオン コーポレーション Bismuth-thiol as a disinfectant for agricultural, industrial and other uses
JP2020527595A (en) * 2017-07-15 2020-09-10 ヴィットハル サワント、アルン New crop fortification, nutritional and crop protection compositions
KR20210003864A (en) * 2018-04-30 2021-01-12 스톨러 엔터프라이지즈, 인크. Synergistic formulation containing at least one gibberellin compound and salicylic acid
KR20210005582A (en) * 2018-04-19 2021-01-14 리다 플랜트 리서치 에스.엘. Synergistic Mixture of Plant Defense Inducers (SYNERGISTIC MIXTURE OF PLANT DEFENSE INDUCTORS)
KR20210006987A (en) * 2018-05-10 2021-01-19 아룬 비탈 사완트 New crop nutrition and fortification composition

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013535506A (en) * 2010-08-12 2013-09-12 マイクロビオン コーポレーション Bismuth-thiol as a disinfectant for agricultural, industrial and other uses
JP2020527595A (en) * 2017-07-15 2020-09-10 ヴィットハル サワント、アルン New crop fortification, nutritional and crop protection compositions
KR20210005582A (en) * 2018-04-19 2021-01-14 리다 플랜트 리서치 에스.엘. Synergistic Mixture of Plant Defense Inducers (SYNERGISTIC MIXTURE OF PLANT DEFENSE INDUCTORS)
KR20210003864A (en) * 2018-04-30 2021-01-12 스톨러 엔터프라이지즈, 인크. Synergistic formulation containing at least one gibberellin compound and salicylic acid
KR20210006987A (en) * 2018-05-10 2021-01-19 아룬 비탈 사완트 New crop nutrition and fortification composition

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116515331A (en) * 2023-05-27 2023-08-01 烟台凯多海洋生物研究院有限公司 Preparation and application methods of apple bagging-free film agent

Also Published As

Publication number Publication date
KR102431000B1 (en) 2022-08-11

Similar Documents

Publication Publication Date Title
US11617370B2 (en) Melanoidins and their use for improving properties of plants
TWI398218B (en) Use of prolines for improving growth and/or yield
KR20160079013A (en) Strigolactone formulations and uses thereof
WO2023003410A1 (en) Composition for autoimmune builder of plants and usage method using same
WO2019172277A1 (en) Composition for inducing plant disease damage resistance or preventing plant disease damage
CN110959617A (en) Plant growth stress protective agent and use method thereof
CN109845760B (en) Application of photosynthetic bacteria in preventing and treating winter jujube diseases
CN101258853A (en) Carbamate and azoles fungicide composition and use
CN112602727A (en) Preparation and application method of plant protective agent
Ellis et al. The development of an action threshold for cabbage aphid (Brevicoryne brassicae) in oilseed rape in the UK
US11839218B2 (en) Methods for treating a fungal infection on a stone fruit tree
KR101431330B1 (en) Bacterial Wilt Preventing Composition and The Preparation Method Thereof
CN111296485B (en) Application of inducer in preventing and treating gray mold of strawberry, increasing chlorophyll content and reducing cell membrane permeability
WO2018236716A1 (en) Compositions and methods for increasing crop yield
CN114176089B (en) Pesticide composition for resisting Chinese rose stem tumor and application thereof
CN115581234B (en) Application of vanillic acid as plant source medicament in preventing and treating larch tip blight
KR102604427B1 (en) Composition for controlling plant diseases comprising Trichoderma longibrachiatum strain and method to control plant diseases
WO2021187815A1 (en) Microbial formulation for enhancing environmental stress tolerance of plants, containing flavobacterium sp. strain tch3-2 as active ingredient, and use thereof
Kumari et al. Brassinosteroids: The Master Regulators
Porlingis et al. Paclobutrazol decreases the harmful effect of high temperatures on fruit set in olive trees
KR20230137238A (en) Composition for preventing plant virus comprising glycomacropeptide with silaic acid as active gradient
KR20230137237A (en) Composition for preventing plant virus comprising organic iodine or organic iodine and sulfur as active gradient
Dianyu et al. Antifungal Activities of Eight Fungicides against Fusarium oxysporum Schlecht
CN112552120A (en) Special foliar fertilizer for rosa roxburghii tratt and application thereof
Raj Chapter-7 Organic Approaches for Grape Vine Disease Management

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22846280

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE