CN115581234B - Application of vanillic acid as plant source medicament in preventing and treating larch tip blight - Google Patents
Application of vanillic acid as plant source medicament in preventing and treating larch tip blight Download PDFInfo
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- CN115581234B CN115581234B CN202211151559.5A CN202211151559A CN115581234B CN 115581234 B CN115581234 B CN 115581234B CN 202211151559 A CN202211151559 A CN 202211151559A CN 115581234 B CN115581234 B CN 115581234B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
Application of vanillic acid as plant source medicament for preventing and treating larch tip blight is provided. The invention relates to an application of vanillic acid, which aims to solve the problems that pesticide residues are easy to generate and the environment is polluted when chemical pesticides are used for preventing and treating the larch tip blight. The vanillic acid has good antibacterial effect on the pathogen of the larch tip blight, namely the vitis vinifera, and the antibacterial rate can reach 30-90% at the concentration of 1-5 mg/ml. The application of the vanillic acid can effectively inhibit the Botrytis cinerea and reduce the dependence on chemical agents in the control process. The invention is applied to the field of plant source bacteriostats.
Description
Technical Field
The invention relates to application of vanillic acid as a plant source medicament in preventing and treating larch tip blight.
Background
Larch has high growth speed and excellent low temperature and drought resistance, is widely distributed in northeast, north China, northwest and southwest areas of China, and is a tree species formed by main forests of mountain needle forests such as northeast China, inner Mongolia and the like. The larch has the functions of soil conservation and wind prevention, and can be used as an excellent tree species of wind prevention and sand fixation forests, water and soil conservation forests and water conservation forests. The plant has developed root system and strong wind resistance, and can be used as a main tree species for forest updating and barren mountain forestation in mountain areas of river basin. Larch wood has the excellent characteristics of compact structure, tough material, strong corrosion resistance and the like, is an important industrial and building material, and has higher economic value.
The shoot rot of larch caused by the Botrytis cinerea (now more named clostridium septemlobum Neofusicoccum laricinum, fall She Songxin) is one of the important diseases of larch, and the disease is serious and rapidly spread, and is a dangerous quarantine disease. In 1938, larch tip blight was found in North sea duct, japan, and the damaged larch developed from the original individual plant to a large-area damage. The fall pine artificial forest removed and burned by the disease in the North sea duct area 1960-1969 reaches 9700 hectares. The leaf-loss and tip-blight of larch occurs in china in the early 70 s, and after ten years, it has spread over a vast forest area in the three northeast provinces. According to the investigation, the average annual loss growth per hectare of larch for 9 years with continuous incidence is 2.04 cubic meters, which is about 60% of the average growth per hectare in 9 years of the stand.
The larch shoot blight is the current year shoot of harmful larch, and the current year shoot from seedling, sapling to forestation can occur, but the sapling has heavier occurrence. Dahurian larch (Larix gmelini), larch (l. Olgensis), larch (l. Pritis-rupprechtii) and larch (l. Kaempferi) are all susceptible to the disease, and the degree of the disease of the rest larch is higher except that the disease of the larch is lighter. The infected larch initially fades in the delignified young shoot or shaft portion, and then gradually changes from light brown to dark brown. The branches have resin overflowed, the upper parts are bent into a hook shape, the needle leaves wither, most of the branches fall off, and only a cluster of needle leaves remains at the top. The shoots above the disease parts die, and the seedlings become non-topped seedlings. In spring of the next year, the side bud branches replace the original main shoots, the disease is happened in successive years, the damage is serious, the five-flower heads and broom-shaped branches are in clusters on the crowns, the high growth stops, the dead of the young trees or the whole plants is formed, and the growth of larch is seriously influenced. The disease is serious and spread rapidly, and has been listed as a forest plant quarantine object. At present, the most effective way for preventing and treating the diseases is chemical prevention and treatment, but abuse of chemical pesticides can cause problems of ecological environment deterioration, reduced biodiversity and the like, and the residue of the pesticides seriously threatens human health.
Disclosure of Invention
The invention aims to prevent and treat the larch tip disease, solves the problems that chemical pesticides are easy to produce pesticide residues and pollute the environment when used for preventing and treating the larch tip disease, and provides application of vanilla acid serving as a plant source medicament in preventing and treating the larch tip disease.
The invention discloses application of vanillic acid as a plant source medicament in preventing and treating larch tip blight.
The invention has the advantages compared with the prior art:
vanillic acid (4-hydroxy-3-methoxybenzoic acid) with molecular formula of C 8 H 8 O 4 . The invention uses in vitro bacteriostasis experiment to verify the bacteriostasis activity of the vanillic acid on the grape trellis fungus (clostridium drosophorae She Songxin) so as to provide a plant source medicament for preventing and controlling the leaf blight of larch.
The invention has the beneficial effects that the Botrytis cinerea (clostridium concha of She Songxin) is a fungus, can generate spores, has stronger infection, propagation and diffusion capability and has control difficulty relative to bacteria. The vanillic acid has good antibacterial effect on the pathogenic pathogen of larch tip blight, namely, the botrytis cinerea of larch (clostridium septemlobum of She Songxin), and the antibacterial rate can reach 30-90% at the concentration of 1-5 mg/ml. The application of the vanillic acid can effectively inhibit the Botrytis cinerea (clostridium septemlobum She Songxin) and reduce the dependence on chemical agents in the control process.
Drawings
FIG. 1 is a comparison of lawn diameters of a treated group and a control group in an in vitro bacteriostasis experiment in example 1;
FIG. 2 is a comparison of lawn status of the treated group and the control group in the in vitro bacteriostasis test in example 1.
Detailed Description
The first embodiment is as follows: the application of the vanillic acid in the embodiment as a plant source medicament in preventing and treating the larch tip blight.
The relative molecular mass of vanillic acid (4-hydroxy-3-methoxybenzoic acid) of this embodiment is 168.15, and the molecular formula is:
this embodiment found that vanillic acid was able to inhibit the growth of Botrytis cinerea (Clostridium roseum She Songxin). The bacteriostasis rate can reach 30-90% at the concentration of 1-5 mg/ml. The vanilloid is shown to have an important role in preventing and controlling the Botrytis cinerea (clostridium septemlobum She Songxin), has excellent environment-friendly characteristics, and can reduce the use of chemical agents.
The current name of Botrytis cinerea in this embodiment is Clostridium falling She Songxin.
The second embodiment is as follows: the first difference between this embodiment and the specific embodiment is that: vanillic acid inhibits the growth of the pathogen, botrytis cinerea, larix Gmelini (Clostridium roseum She Songxin). The other steps are the same as in the first embodiment.
And a third specific embodiment: this embodiment differs from the first or second embodiment in that: the preparation method of vanillic acid as plant source medicament is to dissolve vanillic acid in dimethyl sulfoxide to prepare vanillic acid stock solution, and then dilute with deionized water to prepare working solution. The other steps are the same as those of the first or second embodiment.
The specific embodiment IV is as follows: the present embodiment differs from the first to third embodiments in that: the concentration of the working solution is 1-5 mg/mL. The other steps are the same as those of the first to third embodiments.
Fifth embodiment: this embodiment differs from the first to fourth embodiments in that: the vanillic acid is also used as a plant source medicament for inhibiting the growth of cercospora pinicola, cercospora armandii, cercospora colletotricha, cercospora subtilis and cercospora pinicola. Other steps are the same as those of the first to fourth embodiments.
The following examples are used to verify the benefits of the present invention:
example 1:
the application effect of the vanilloid serving as a plant source medicament in inhibiting the growth of the Botrytis cinerea (clostridium perfringens of She Songxin) is verified.
Biological material preparation
The Botrytis cinerea (clostridium drop She Songxin) is inoculated to a quantitative Potato Dextrose Agar (PDA) culture medium (8 ml/dish), and is cultivated at a constant temperature of 25 ℃ and in a dark place, and after the plates are grown up for later use.
(II) dissolution of the agent and inoculation of pathogenic bacteria
200mg of vanillic acid was dissolved in 1ml of dimethyl sulfoxide (DMSO) to obtain a stock solution of vanillic acid. The treatment group is that 1mL of vanilla acid stock solution is added into 99mL of dissolved PDA culture medium, and the mixture is uniformly mixed, and the final concentration is 2mg/mL; the control group was prepared by adding 1ml of DMSO to 99ml of dissolved PDA medium and mixing. The temperature of the culture medium should be reduced to below 50 ℃ before dosing. The culture medium after the drug addition is quantitatively split into disposable culture dishes (8 ml/dish) with the diameter of 60mm, and after the culture medium is condensed, the Botrytis cinerea (clostridium perfringens of She Songxin) is inoculated.
A round lawn of 5mm diameter was inoculated onto the medium containing the agent (treated group) and the control medium (control group) at the edge of the previously prepared Botrytis cinerea (Clostridium roseum She Songxin). Treatment and control groups were each set to 6 replicates. Culturing at 25deg.C under constant temperature and in dark place.
(III) statistics of bacteriostasis degree
The lawn diameters of the treated group and the control group were measured by the crisscross method, and the lawn growth state was recorded by photographing. The bacteriostasis rate was counted according to the following formula.
The test results showed that the lawn diameter of the treated group was significantly smaller than that of the control group (fig. 1, wherein ■ is the control group,
to treat groups), it was demonstrated that treatment with vanillic acid inhibited growth of the bacteria. Second, the mycelium of Botrytis cinerea (Clostridium roseum She Songxin) was fluffy in the control medium and grew normally, whereas the mycelium growth was inhibited in the treatment medium (FIG. 2). After 3-7 d of treatment, the bacteriostasis rate of the vanillic acid is always maintained to be above 75%. After 4d treatment, the bacteriostasis rate of the vanillic acid is highest and reaches 88.64 percent. After 13d of treatmentThe bacteriostasis rate of the vanillic acid can still reach 48.15 percent.
Example 2:
the application of the vanilloid as a plant source medicament in preventing and controlling the larch tip blight is verified.
A suspension of Botrytis cinerea (Clostridium falling She Songxin) spores was prepared. The falling She Songmiao tip was needle punched. The spore suspension was applied to larch Miao Shangkou and the inoculation area was covered with a plastic bag for 3d. Vanilla agent (2 mg/ml) was sprayed onto larch seedlings after 14d inoculation, DMSO agent at the same concentration was used as a control, and after 7d treatment, the disease condition was observed on time as She Songmiao, and the disease index was counted.
The disease classification criteria are as follows: grade 0 is healthy plants; the stem of the branch is chlorosis, and the disease plant with few needle leaves falling off is a grade I disease plant; the stem part of the branch is yellow brown, half of needle leaves fall off, and the disease plant with slightly drooping tip is a II-level disease plant; the stem of the branch is brown, most of needle leaves fall off, and the disease plant with the drooping top is III-level disease plant; the stem part of the branch is black brown, the tip head sags, and the disease plants with all the needle leaves falling off except the tip head, a bundle of dark gray dead needle leaves are IV-level disease plants.
The disease index formula:
after the treatment of vanillic acid, the disease state is inhibited. The calculation shows that after 7d of treatment, the disease index of the treatment group is 55.56% of that of the control group, the control effect of the vanillic acid is 44.44%, and the treatment group and the control group have extremely obvious difference (p=0.0003).
In summary, the invention provides a new plant source medicament vanillic acid which can inhibit the growth of the pathogenic larch grape seat cavity fungus (clostridium perfringens She Songxin) of the larch tip disease, and if the novel plant source medicament is popularized and put into use on a large scale, the novel plant source medicament has the advantages of greatly reducing the dependence on chemical agents, along with safety, environmental protection and environmental friendliness.
Claims (4)
1. Application of vanillic acid as plant source medicament for preventing and treating larch tip blight is provided.
2. Use of vanillic acid according to claim 1, characterized in that the vanillic acid inhibits the growth of the pathogenic species viticola lardii of the shoot tip disease.
3. The use of vanillic acid according to claim 1, wherein said vanillic acid is prepared as a plant source by dissolving vanillic acid in dimethyl sulfoxide to prepare a stock solution of vanillic acid and diluting with deionized water to prepare a working solution.
4. The use of vanilloid as defined in claim 3, wherein the concentration of the working fluid is 1-5 mg/mL.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2022101509494 | 2022-02-18 | ||
| CN202210150949.4A CN114467940A (en) | 2022-02-18 | 2022-02-18 | Application of vanillic acid as botanical drug in prevention and treatment of larch dieback disease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115581234A CN115581234A (en) | 2023-01-10 |
| CN115581234B true CN115581234B (en) | 2023-06-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210150949.4A Withdrawn CN114467940A (en) | 2022-02-18 | 2022-02-18 | Application of vanillic acid as botanical drug in prevention and treatment of larch dieback disease |
| CN202211151559.5A Active CN115581234B (en) | 2022-02-18 | 2022-09-21 | Application of vanillic acid as plant source medicament in preventing and treating larch tip blight |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210150949.4A Withdrawn CN114467940A (en) | 2022-02-18 | 2022-02-18 | Application of vanillic acid as botanical drug in prevention and treatment of larch dieback disease |
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- 2022-02-18 CN CN202210150949.4A patent/CN114467940A/en not_active Withdrawn
- 2022-09-21 CN CN202211151559.5A patent/CN115581234B/en active Active
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| Publication number | Publication date |
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| CN114467940A (en) | 2022-05-13 |
| CN115581234A (en) | 2023-01-10 |
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