KR102431000B1 - Composition for an autoimmune builder of plants containing aspirin and benzothiazole, and method of using the same - Google Patents

Abstract

The present invention relates to an autoimmune enhancing composition of a plant containing active ingredients of aspirin and benzozoazole. Aspirin (acetylsalicylic acid, ASA) and benzozoazole are dissolved together to form an autoimmune enhancing composition of a plant, and the enhancing composition is sprayed or provided on the surface of leaves, stems and fruits of plants by a conventional spraying method, which enhances the autoimmune function against external environmental stress such as drought, soil salinity, freezing, cold tolerance and heat during plant growth. Accordingly, the enhancing composition can provide high quality and productivity increase of crops by improving resistance to disease. The autoimmune enhancing composition of the present invention is provided as an autoimmune enhancing composition of plant, which is prepared by dissolving 1.5 to 5.0 wt % of aspirin (acetylsalicylic acid, ASA) and 0.5 to 2.0 wt % of benzothiazole in water, and dissolving 0.05 to 0.25 wt % of a preservative, 0.45 to 3.5 wt % of a penetrating agent, and 0.10 to 0.50 wt % of an anti-foaming agent. The present invention relates to a technology relates to autoimmune enhancement increment of plants, wherein after diluting the autoimmune enhancing agent composition in water in the range of 1/150 to 1/1,500, manual and automated spraying equipment is used to spray the enhancing agent to the leaves, stems, and fruits of plants to increase autoimmunity of plants.

Description

Composition for an autoimmune builder of plants containing aspirin and benzothiazole, and method of using the same

The present invention provides an autoimmune enhancer composition for plants in which aspirin (acetylsalicylic acid, ASA) and benzothiazole are dissolved together in water, and the enhancer composition is applied to the leaves, stems and fruits (fruits) of plants by a conventional spraying method. By spraying/providing on the surface, and enhancing the autoimmune function against external environmental stresses such as drought, soil salinity, freezing, cold resistance, and heat during the growth process of plants, ultimately, the high quality and quality of crops and It relates to a plant autoimmune enhancer composition comprising active ingredients of aspirin and benzozoazole, which can provide increased productivity.

Plants are constantly subjected to stress from their surroundings during their survival period from germination to death. Unlike animals, plants do not have the ability to move away from these stresses. need to be established

The stress experienced by plants can be largely divided into biological stress derived from pests and abiotic stress derived from the environment. These have been pointed out as the main cause of declining production of food crops and energy crops.

Therefore, efforts by botanists to increase the stress resistance of plants and improve crop yields through genetic manipulation through the understanding of these stress signaling mechanisms are continuing.

Recently, the reduction of the ozone layer caused by environmental pollution has resulted in a sharp increase in the amount of UV reaching the earth. Studies have shown that the concentration is lowered, along with lowering blood pressure and heart rate.

However, plants that relieve human stress also experience stress. It is analyzed that the reason why grass does not grow on the hiking trails in the mountains is because of plant stress. It is confirmed that the energy is being lost by exhaling the energy. That is why the inner grass, which is less brushed by the side of the road, where people often visit, is taller.

Ethylene is a plant hormone that ripens fruits or accelerates the aging of plant tissues, and also acts to inhibit cell division and length growth of plants. Ethylene's growth inhibitory effect has been proven in several previous experiments. thickens This is because the young leaves around the growth point are physically stimulated to generate ethylene.

If the plants of the experimental control group were shaken daily under the same growth conditions or beaten with a duster, etc., instead of growing shorter than the plants of the normal group, the stems became thicker, and the young leaves around the growth point were physically stimulated to generate ethylene. This is the reason why the trees growing on the coast, which receive more water, are short and do not grow upright.

It is also the reason why orchid growers make it a taboo to move pots and are sensitive to others touching their eggs.

When the total amount of food produced by crops is taken as 100%, the total crop production lost due to environmental stress such as drought, cold damage, and salt damage is about 69%. It can be said that the actual output that can be obtained is only 21%.

Therefore, plant stress management is a very urgent task for mankind, which is facing problems such as the ever-increasing population and reduction of arable land. But now the Earth is moving towards a situation that causes more stress on plants. That's because of climate change.

As the average temperature rises, the evapotranspiration loss of lakes and reservoirs increases, leading to higher salinity. In addition, a decrease in precipitation decreases the flow of rivers in many areas, further increasing salinity accumulation, which in turn leads to salinization of the soil.

Currently, 20% of all plants and 33% of arable land worldwide are subjected to the salinization stress of these soils. Globally, more and more crops will face high salinity stress due to climate change.

Various environmental stresses have a great influence on plant growth, development and productivity. Therefore, it is very important agriculturally to increase the resistance to these environmental stresses.

Therefore, reducing the crop loss caused by plant stress by even 1% is expected to dramatically improve future food problems. Therefore, plant stress management is as important as human stress.

Until now, various stresses provided by the environment have a great influence on the growth, development and productivity of plants. Therefore, a method for coping with the stress of plants is being devised, but the efficacy against it is not very high.

Looking at the conventional means for resolving stress in plants, in Korean Patent Laid-Open Publication No. 10-2021-0085328 A, Plant-growth-promoting bacteria (PGPR), Penibacillus yonginensis DCY84T (Paenibacillus yonginensis DCY84T) A composition for inhibiting salt stress of ginseng containing a strain is presented.

Korean Patent Laid-Open Publication No. 10-2021-0069224 A proposes a Chinese cabbage-derived gene having resistance to drought and high temperature stress of a plant including the BrPP5 gene consisting of the nucleotide sequence of SEQ ID NO: 1 and uses thereof.

Korean Patent Laid-Open No. 10-2021-0051902 A proposes a novel promoter based on the characteristics of a drought stress-specific cis-regulatory element and a method for preparing the same.

In Korea Patent Laid-Open Publication No. 10-2021-0048433 A, a novel gene ( MARP1) and its uses are presented.

Korean Patent Application Laid-Open No. 10-2019-0133422 proposes a rice-derived OsCP1 (Oryza sativa cysteine proteinase 1) gene that regulates salt stress tolerance of plants and uses thereof.

Korean Patent Application Laid-Open No. 10-2019-0162699 discloses a yeast Ypt1p mutant protein composition that knocks out heat stress resistance.

US Patent Application Publication No. 16/267910 proposes a composition for plant growth and resistance in order to stimulate germination in agricultural crops.

US Patent Publication No. 2021-0180074 A1 discloses polynucleotides for improving abiotic stress-tolerant plants and abiotic stresses and methods.

US Patent Publication No. 2020-0095595 A1 proposes a method for producing high-yield crops under abiotic stress conditions.

US Patent Publication No. 2020-0208053 A1 proposes a method for reducing water stress in plants.

US Patent Publication No. 2021-0015095 A1 proposes a method for improving stress resistance, growth and production in plants.

US Patent Publication No. 2018-0360039 A1 proposes a low molecular weight compound for enhancing plant stress resistance.

Japanese Laid-Open Patent Publication No. 2021-093933 A proposes a method of imparting environmental stress tolerance to plants by contacting active oxygen to plants on the due date of flowering.

In Japanese Laid-Open Patent Publication No. 2018-525440, amide 7-carboxy benzo [1,2,3] thiadiazole and its derivatives containing plant stimulants control plant growth, control plant metabolic processes, or plants It proposes an application method that can regulate the physiological processes of the plant, prevent the effects of biotic stress on plants, or prevent the effects of abiotic stress on plants.

Japanese Laid-Open Patent Publication No. 2018-511562 discloses a 2-oxo-3,4-dihydroquinoline compound as a plant growth regulator.

Japanese Laid-Open Patent Application Laid-Open No. 2016-069380 proposes a plant salt tolerance improving agent for improving environmental stress tolerance such as salt stress of plants.

Japanese Patent Laid-Open No. 2017-137281 discloses an agent for improving heat stress tolerance in plants containing allantoin as an active ingredient, a method for improving heat stress tolerance, a bleaching inhibitor, and a DREB2A gene expression promoter.

However, in the prior art presented above, as in the technical idea of the present application, a technical idea similar to the technical idea related to an autoimmune enhancer for plants using aspirin and benzozoazole as active ingredients has not been presented in the prior art, and the prior art is combined. Even when applied, it was confirmed that the present technology is a technology that cannot achieve the intended purpose or effect, and thus led to a patent application.

The present application provides an autoimmune enhancing agent composition of a plant in which aspirin (acetylsalicylic acid, ASA) and benzothiazole are dissolved together in water by a spraying method on the surface of leaves, stems and fruits (fruits) of plants, Aspirin can provide high quality and productivity of crops by enhancing the function of autoimmunity against external environmental stresses such as drought, soil salinity, freezing, cold resistance and heat during the growth process, thereby inhibiting infection and improving resistance to disease It is an object of the invention to provide a plant autoimmune enhancer composition containing the active ingredients of benzoazole and benzoazole, and a method of using the same.

The present technology is a means for achieving the above object, that is, as a plant autoimmune enhancer composition for relieving stress provided from the external environment during the process of crop cultivation, aspirin (acetylsalicylic acid, ASA) in water 1.5 to 5.0 wt% And benzothiazole is dissolved in 0.5 to 2.0% by weight, the preservative is 0.05 to 0.25% by weight, the penetrant is 0.45 to 3.5% by weight, and the antifoaming agent is dissolved in 0.10 to 0.50% by weight Autoimmune enhancer composition of a plant provided to achieve

In the autoimmune enhancer composition provided herein, the preservative is at least one preservative selected from among sorbic acid, benzoic acid, paraoxybenzoic acid, propionic acid, methylparaben, propylparaben, phenoxyethanol, diazolidinylurea, and imidazolidinylurea. The penetrant may be used, and the penetrant is sodium naphthalenesulfonate-formalin condensate, sodium alkylnaphthalenesulfonate or its formalin condensate, formalin condensate of sodium phenolsulfonate and sodium naphtholsulfonate, alkyl diphenyl ether disulfonate sodium, sodium ligninsulfonate, non Ionic surfactant polyoxyethylene alkyl (allyl) ether, sodium polyacrylate, olefin-sodium maleate copolymer, condensed phosphate, alkylbenzene (naphthalene) sulfonate, sodium dioctylsulfon maleate, partial amide of styrene-maleic anhydride copolymer One or more penetrants may be selected and used among esterified/partially esterified products, and the antifoaming agent may be a silicone-based antifoaming agent mainly composed of a straight-chain silicone oil such as dimethylpolysiloxane, methylphenylpolysiloxane, or methylvinylpolysiloxane.

The present application is a method for enhancing autoimmunity of plants for relieving stress provided by the external environment during crop cultivation, wherein 1.5 to 5.0% by weight of aspirin (acetylsalicylic acid, ASA) and 0.5 to 2.0% by weight of benzothiazole in water The weight% is dissolved, the preservative is 0.05 to 0.25% by weight, the penetrant is 0.45 to 3.5% by weight, and the antifoaming agent is dissolved in 0.10 to 0.50% by weight to form an autoimmune enhancer composition of a plant, wherein the autoimmune enhancer composition is 1/ After dilution in water in the range of 150 to 1/1,500, manual and automated spraying equipment is used to spray the leaves, stems, and fruits of the plant to increase the autoimmunity of the plant. is initiated.

Here, aspirin (acetylsalicylic acid, ASA) aims to provide an autoimmune function against external environmental stress. You can purchase and use the used aspirin drug, but it is preferable to manufacture and use it as much as possible because it is used in a wide range of plant autoimmune fields and requires a large amount.

In addition, the benzothiazole aims to not only double the autoimmune function of aspirin to provide an autoimmune function against external environmental stress, but also activate the autoimmune function of stress to various external environments, It is preferable to purchase and use drugs that are sold without special restrictions.

The preservative is the organic matter contained in the autoimmune enhancer composition of plants containing aspirin and benzozoazole due to the occurrence of microorganisms or fungi over the passage of appropriate temperature and period. The penetrating agent is used for the purpose of enhancing the penetration rate of the autoimmune enhancer active ingredient of aspirin and benzozoazole sprayed on the stem, leaf, and fruit (fruit) of the plant into the stem, leaf, and fruit (fruit) epidermal tissue of the plant. The antifoaming agent is used for the purpose of increasing the effect of the autoimmune enhancer by It is used for the purpose of preventing

Therefore, the present invention provides a function of autoimmunity against external environmental stresses such as drought, soil salinity, freezing, cold resistance and heat during plant growth by means of a plant autoimmune enhancer composition containing active ingredients of aspirin and benzoazole. A plant autoimmune enhancer composition that can provide high quality and productivity increase of crops according to infection suppression and disease resistance improvement by enhancing it, and a method of using the same can sufficiently overcome external environmental stress, which is becoming more extensive, so that all crops can be It is expected to be widely applied to growing farms.

In the prior art, the autoimmune efficiency related to stress is not high, so due to the various stresses provided by the environment so far, the yield is low due to the great influence on the growth, development and productivity of plants. have.

However, the plant's autoimmune enhancer can sufficiently solve the problems of various stresses encountered by plants due to changes in the external environment, so that high-quality crops can be obtained in large numbers, thereby greatly solving future food problems as well as quality. It is expected that it will greatly contribute to the increase of farm household income due to the effect that can contribute to economic income for production activities of farm households by being able to maintain high-quality marketability through improvement.

Prior to describing the embodiments of the invention for implementing the technical idea of the present application, the terms or words used in the specification or claims of the present application should not be construed as being limited to conventional or dictionary meanings, The protection scope of the present application should be interpreted in meaning and concept consistent with the technical spirit of the present invention, and the examples described in the present specification are only the most preferred embodiment of the present invention and do not represent all the technical ideas of the present invention. , it should be understood that there may be various equivalents and modifications that can be substituted for them at the time of the present application.

Hereinafter, the present invention will be described in detail with reference to the following examples to which the technical idea of the present application is applied.

Salicylic acid (Samjeon Pure Yak) and glacial acetic acid were mixed, synthesized under a phosphoric acid catalyst, and left in a refrigerator at 2 oC for one day to obtain an aspirin precipitate, which was used as an aspirin source according to the following examples.

Example 1

Aspirin as the active ingredient of the plant autoimmune enhancer is 1.5 kg, benzozoazole (Samjeon pure medicine) 0.05 kg, benzoic acid (Samjeon pure medicine) 0.05 kg as a preservative, sodium alkyldiphenyl ether disulfonate [Miwon Co., Ltd.] 0.5 kg as a penetrating agent, antifoaming agent After preparing a plant autoimmune booster composition consisting of 0.1 kg of dimethylpolysiloxane [Blue Mount Technology Co., Ltd.], dilute it to 1/200, and sprinkle sprinklers on the leaves, stems and fruits of 450 watermelons grown in a 660 m2 plastic house. It was sprayed once every 2 days for 120 days, and the average weight of 450 watermelons was confirmed.

Example 2

Aspirin as the active ingredient of the plant autoimmune enhancer is 3.0 kg, benzozoazole (Samjeon pure medicine) 0.15 kg, methylparabe (Samjeon pure medicine) 0.10 kg as a preservative, sodium polyacrylate [Han Nong Hwaseong] 2.0 kg as a penetrating agent, as an antifoaming agent Dimethylpolysiloxane [Blue Mount Technology Co., Ltd.] After preparing a plant autoimmune booster composition consisting of 0.25 kg, it was diluted 1/750 and 1 for 100 days using a sprinkler on about 8,000 Chinese cabbages grown in a 660 m2 plastic house. It was sprayed twice/day, and the average weight of 8,000 Chinese cabbages was confirmed.

Example 3

Aspirin as the active ingredient of the plant autoimmune enhancer is 5.0 kg, benzozoazole (Samjeon pure medicine) 0.25 kg, phenoxyethanol (Samjeon pure medicine) 0.25 kg as a preservative, olefin-sodium maleate copolymer [Aekyung Chemical Co., Ltd.] 3.5 kg as a penetrant , as an antifoaming agent, prepare an autoimmune increasing agent composition for plants consisting of 0.5 kg of dimethylpolysiloxane [Blue Mount Technology], and then diluted to 1/1,500, and a single grape for 180 grapes of Campbell variety grown in a 660 m2 plastic greenhouse The average weight was confirmed.

Comparative Examples 1-3

It was carried out in the same manner as in Examples 1 to 3 using pure tap water instead of the plant autoimmune adjuvant composition.

Table 1 shows the results of Comparative Examples 1-3 and Examples 1-3.

division average weight for harvested crops Watermelon (kg/piece) Chinese cabbage (kg/abandoned) Grapes (g/one bunch) Example 1 9.83 - - Example 2 - 5.11 - Example 3 - - 336 Comparative Example 1 7.16 - - Comparative Example 2 - 3.52 - Comparative Example 3 - - 284

As shown in Table 1, in Comparative Examples 1 to 3, in which pure tap water was supplied to the crop without the plant autoimmune adjuvant composition being provided to the leaves, stems, and fruits of the plant, the average weight of the watermelon was 7.16 kg, and the average weight of the Chinese cabbage was 7.16 kg. A crop with an average weight of 3.52 kg per bunch in the case of grapes and an average weight of 284 g per bunch in the case of grapes was harvested.

On the other hand, in Examples 1 to 3, when the plant autoimmune adjuvant composition was sprayed and coated on watermelon, Chinese cabbage, and grape crops, the average weight of watermelon was 9.83 kg, and in the case of Chinese cabbage, it was 5.11 kg per leaf, In the case of grapes, 336 g per bunch is shown, and when the composition composed of aspirin and benzozoazole, which are active ingredients of the plant autoimmune enhancer of the present application, is coated on the leaves, stems, and fruits of plants during the crop cultivation process, external It was confirmed that crops with the highest quality can be cultivated by solving the stress on the environment.

Therefore, due to the various stresses on the external environment that occur during the conventional crop cultivation process by the technical configuration of the present application, it is expected that the marketability will be greatly reduced and the yield will be lowered, so that future food problems including huge economic loss for farmers can be sufficiently overcome. .

The reason the upper and lower limits of the raw material components described in the claims of the present application are set is that when the upper limit is exceeded, the effect provided by the composition is insignificant compared to the increase in the manufacturing cost of the immune enhancing composition, and when applied below the lower limit, the present application This is because it is preferable to use in the upper and lower limits of the raw material ingredients described in the claims of the present application because the immunity enhancement ability to be obtained from the is significantly lowered.

Claims (5)

In the autoimmune enhancer composition of a plant for relieving the stress provided from the external environment during the process of growing crops,
1.5 to 5.0 wt% of aspirin (acetylsalicylic acid, ASA) and 0.5 to 2.0 wt% of benzothiazole are dissolved in water,
0.05 to 0.25 wt% of a preservative selected from sorbic acid, benzoic acid, paraoxybenzoic acid, propionic acid, methylparaben, propylparaben, phenoxyethanol, diazolidinylurea, and imidazolidinylurea;
Sodium naphthalenesulfonate-formalin condensate, sodium alkylnaphthalenesulfonate or its formalin condensate, formalin condensate of sodium phenolsulfonate and sodium naphtholsulfonate, sodium alkyl diphenyl ether disulfonate, sodium lignin sulfonate, nonionic surfactant polyoxyethylene Alkyl (allyl) ether, sodium polyacrylate, olefin-sodium maleate copolymer, condensed phosphate, alkylbenzene (naphthalene) sulfonate, sodium dioctyl sulfon maleate, styrene-maleic anhydride partially amidated / partially esterified 0.45 to 3.5% by weight of the penetrant
An autoimmune enhancer composition for plants, wherein the antifoaming agent is provided to form an autoimmune enhancer composition for plants, wherein the antifoaming agent is dissolved in an amount of 0.10 to 0.50% by weight. delete delete According to claim 1,
The antifoaming agent is an autoimmune enhancer composition for plants, characterized in that a silicone-based antifoaming agent selected from dimethylpolysiloxane, methylphenylpolysiloxane, and methylvinylpolysiloxane is used. In the method for enhancing autoimmunity of plants for relieving stress provided from the external environment during the process of growing crops,
1.5 to 5.0 wt% of aspirin (acetylsalicylic acid, ASA) and 0.5 to 2.0 wt% of benzothiazole are dissolved in water,
0.05 to 0.25 wt% of a preservative selected from sorbic acid, benzoic acid, paraoxybenzoic acid, propionic acid, methylparaben, propylparaben, phenoxyethanol, diazolidinylurea, and imidazolidinylurea;
Sodium naphthalenesulfonate-formalin condensate, sodium alkylnaphthalenesulfonate or its formalin condensate, formalin condensate of sodium phenolsulfonate and sodium naphtholsulfonate, sodium alkyl diphenyl ether disulfonate, sodium lignin sulfonate, nonionic surfactant polyoxyethylene Alkyl (allyl) ether, sodium polyacrylate, olefin-sodium maleate copolymer, condensed phosphate, alkylbenzene (naphthalene)sulfonate, sodium dioctylsulfonmaleate, partial amidation/partial esterification of styrene-maleic anhydride copolymer 0.45 to 3.5% by weight of the penetrant
A silicone-based antifoaming agent selected from dimethylpolysiloxane, methylphenylpolysiloxane, and methylvinylpolysiloxane is dissolved in 0.10 to 0.50 wt% to form an autoimmune enhancing agent composition for plants
Dilute with water 150 to 1,500 times the weight of the autoimmune enhancer composition and spray on the leaves, stems, and fruits of the plant by mobilizing manual and automated spraying equipment, characterized in that it is applied to increase the autoimmunity of the plant Autoimmune enhancement method.