WO2022223652A1 - Procédé de séchage pour hmos - Google Patents

Procédé de séchage pour hmos Download PDF

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Publication number
WO2022223652A1
WO2022223652A1 PCT/EP2022/060470 EP2022060470W WO2022223652A1 WO 2022223652 A1 WO2022223652 A1 WO 2022223652A1 EP 2022060470 W EP2022060470 W EP 2022060470W WO 2022223652 A1 WO2022223652 A1 WO 2022223652A1
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WO
WIPO (PCT)
Prior art keywords
hmo
drying process
lacto
drying
process according
Prior art date
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PCT/EP2022/060470
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English (en)
Inventor
Pierre CHASSAGNE
Josef HOERMANSPERGER
Sabrina KELLER
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Dsm Ip Assets B.V.
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Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of WO2022223652A1 publication Critical patent/WO2022223652A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
    • F26B21/02—Circulating air or gases in closed cycles, e.g. wholly within the drying enclosure
    • F26B21/04—Circulating air or gases in closed cycles, e.g. wholly within the drying enclosure partly outside the drying enclosure
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
    • F26B21/06—Controlling, e.g. regulating, parameters of gas supply
    • F26B21/08—Humidity
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
    • F26B21/06—Controlling, e.g. regulating, parameters of gas supply
    • F26B21/10—Temperature; Pressure
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
    • F26B21/14—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects using gases or vapours other than air or steam, e.g. inert gases
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B3/00—Drying solid materials or objects by processes involving the application of heat
    • F26B3/02—Drying solid materials or objects by processes involving the application of heat by convection, i.e. heat being conveyed from a heat source to the materials or objects to be dried by a gas or vapour, e.g. air
    • F26B3/06—Drying solid materials or objects by processes involving the application of heat by convection, i.e. heat being conveyed from a heat source to the materials or objects to be dried by a gas or vapour, e.g. air the gas or vapour flowing through the materials or objects to be dried
    • F26B3/08—Drying solid materials or objects by processes involving the application of heat by convection, i.e. heat being conveyed from a heat source to the materials or objects to be dried by a gas or vapour, e.g. air the gas or vapour flowing through the materials or objects to be dried so as to loosen them, e.g. to form a fluidised bed

Definitions

  • the present invention relates to a new method for drying human milk oligosaccharides (HMOs). This method allows to obtain HMOs with a very low water activity.
  • Water activity of a compound (or a formulation) is one the main factor impacting the storage stability over the shelf life of compound (of the formulation) or of a product, wherein such a compound (or formulation) is incorporated.
  • High-water-activity ingredients can be added as long as the total water activity remains below 0.2 or, ideally for long shelf lives, below 0.15 in order to minimize product deterioration as for instance microbial proliferation or non-enzymatic browning. So it is desirable to be able to get HMOs with such a low water activity.
  • the water activity (a w ) is the partial vapor pressure of water in a solution divided by the standard state partial vapor pressure of water.
  • the standard state is most often defined as the partial vapor pressure of pure water at the same temperature. Pure distilled water has a water activity of exactly one.
  • the water activity can be measured by commonly known methods. In the context of the present invention all given water activities are measured by using a Novasina LabMaster at 25° C.
  • HMOs are known to have high hygroscopicity and usually a high water activity (of over 0.2).
  • the storage stability can be affected in a negative manner.
  • HMOs with a water activity of below 0.15 could be obtained.
  • other commonly known and used drying processes such as drying under vacuum
  • the present invention relates to a HMO drying process (DP), wherein at least one HMO is dried by using a fluid bed dryer.
  • DP HMO drying process
  • the HMOs dried with such a method have a water activity below, 0.12, even below 0.10.
  • the HMOs dried with such a method can have a water activity of as low as 0.02.
  • the present invention also relates to HMOs, which are dried by using a fluid bed dryer.
  • the present invention also relates to a HMO having a water activity (a w ) of below 0.15, preferably below 0.12, more preferred below 0.1.
  • HMOs Human milk oligosaccharides
  • HMOs are composed of the five monosaccharides glucose (Glc), galactose (Gal), N- acetylglucosamine (GlcNAc), fucose (Fuc) and sialic acid (Sia), with N-acetylneuraminic acid (Neu5Ac) as the predominant if not only form of Sia. More than two hundred different HMOs have been identified so far.
  • HMOs can be isolated from breast milk or they can be produced chemically or biochemically. HMOs are available commercially from a variety of producers.
  • the source of the HMO is not essential. It is clear that HMOs from different sources can be used.
  • HMOs human immunoglobulin-like compounds
  • modulation of the intestinal microbiota anti-adhesive effect against pathogens
  • modulation of the intestinal epithelial cell response and development of the immune system.
  • HMOs have very positive effect when consumed by humans and/or animals.
  • the present invention relates to a HMO drying process (DP1), which is drying process (DP), wherein HMO is chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl lactose (DFL), Lacto-N- fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 ' Sialyllactose Sodium Salt (6’SL), and Lacto-N-Tetraose (LNT).
  • HMO 2'-fucosyllactose
  • LNnT lacto-N-neotetraose
  • 3FL lacto-N- fucopentaose I
  • LNFP I Lacto-N- fucopentaose I
  • 3'SL Sialyllactose Sodium Salt
  • the present invention relates to a HMO drying process (DPT), which is drying process (DP), wherein HMO is chosen from the group consisting of 2'-fucosyllactose ( 2 ' FL), difucosyllactose (DFL) and lacto-N-neotetraose (LNnT).
  • DPT drying process
  • DP drying process
  • HMO is chosen from the group consisting of 2'-fucosyllactose ( 2 ' FL), difucosyllactose (DFL) and lacto-N-neotetraose (LNnT).
  • the present invention relates to a HMO drying process (DP1”), which is drying process (DP), wherein HMO is 2'-fucosyllactose (2' FL) or difucosyllactose (DFL).
  • DP1 drying process
  • HMO is 2'-fucosyllactose (2' FL) or difucosyllactose (DFL).
  • Fluidized bed dryer (also called fluid bed dryer) is a kind of equipment used very often in the pharmaceutical, chemical and food industries to reduce the moisture content of pow der and granules.
  • the equipment works on a principle of fluidization of the feed materials.
  • the general way of working of the fluid bed drying process is the following:
  • Hot air is introduced into the vessel (product container) at high pressure through a perforated bed of the HMO, which are to be dried.
  • the HMO which are to be dried, are lifted from the bottom and suspended in a stream of air (fluidized state). Heat transfer is accomplished by direct contact between the HMO and hot gases. The vaporized liquid is carried away by the drying gasses. Sometimes to save energy, the exit gas is partially recycled.
  • the present invention relates to a HMO drying process (DP2), which is drying process (DP), (DP1), (DPT) or (DP1”), wherein hot air is introduced into the vessel (prod uct container) at high pressure through a perforated bed of the HMO, which are to be dried, and the HMO, which are to be dried, are lifted from the bottom and suspended in a stream of air (fluidized state) and the heat transfer is accomplished by direct contact between the HMO and hot gases, and the vaporized liquid is carried away by the drying gasses, and optionally the exit gas is partially recycled.
  • DP2 drying process
  • DP1 drying process
  • DP1 drying process
  • hot air is introduced into the vessel (prod uct container) at high pressure through a perforated bed of the HMO, which are to be dried, and the HMO, which are to be dried, are lifted from the bottom and suspended in a stream of air (fluidized state) and the heat transfer is accomplished by direct contact between the HMO and
  • a typical fluid bed dryer usually comprises the following components:
  • the present invention relates to a HMO drying process (DP3), which is drying process (DP), (DP1), (DPT), (DP1”) or (DP2), wherein the fluid bed dryer comprises an air preparatory unit, a product container, an exhaust filter, an exhaust blower and a control panel.
  • DP3 drying process
  • DP1 drying process
  • DP2 drying process
  • DP1 drying process
  • DP2 fluid bed dryer
  • the fluid bed dryer comprises an air preparatory unit, a product container, an exhaust filter, an exhaust blower and a control panel.
  • some parameters need to be controlled. These parameters are usually categorized into 3 categories apparatus parameters, process parameters and product parameters.
  • the apparatus parameters are those, which are controlled by the equipment (the fluid bed dryer).
  • Such apparatus parameters are i.e.: the position and shape of the air distribution plate as well as the shape of the apparatus.
  • the apparatus parameters are not so essential for the process according to the present invention.
  • the process according to the present invention works with commercially avail able fluid bed dryers (e.g. Glatt, GEA, Allgeier, Proceipt, Huttlin, DMR).
  • the process/operating parameter are those, which are controlled by the process.
  • Such process/operating parameters are i.e. temperature of the inlet gas, humidity of the drying gas, the dew point of the drying gas, the airflow rate/gas velocity, duration of the drying process.
  • the temperature of the inlet gas in the process according to the present invention is usually between 10 °C and 120 °C. Preferably between 30 °C and 100 °C. More preferably between 50 °C and 90 °C. Even more preferably between 60 °C and 90 °C.
  • the present invention relates to a HMO drying process (DP4), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2) or (DP3), wherein the temperature of the inlet gas is between 10 °C and 120 °C.
  • the present invention relates to a HMO drying process (DP4’), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2) or (DP3), wherein the temperature of the inlet gas is between 30 °C and 100 °C.
  • the present invention relates to a HMO drying process (DP4”), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2) or (DP3), wherein the temperature of the inlet gas is between 50 °C and 90 °C. Therefore the present invention relates to a HMO drying process (DP4’”), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2) or (DP3), wherein the temperature of the inlet gas is between 60 °C and 90 °C.
  • the humidity of drying gas in the process according to the present invention is usually and preferably ⁇ 20% . More preferably ⁇ 15%.
  • the present invention relates to a HMO drying process (DP5), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”) or (DP4’”), wherein the humidity of drying gas in the process is ⁇ 20%.
  • the present invention relates to a HMO drying process (DP5’), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”) or (DP4’”), wherein the humidity of drying gas in the process is ⁇ 15%.
  • the gas used for the drying in the process according to the present invention is preferably a dried gas having a dew point of below +5° C. In particular having a dew point of from about 0°C to about -50° C.
  • a dew point of +5°C. is equivalent to roughly 5g of water perm of air.
  • compressed air having a dew point of about -25° C or a nitrogen having a dew point of about -40° C can be used.
  • the present invention relates to a HMO drying process (DP6), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5) or (DP5’), wherein drying gas has a dew point of below +5° C.
  • the present invention relates to a HMO drying process (DP6’), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5) or (DP5’), wherein drying gas has a dew point of 0°C -50° C. Therefore the present invention relates to a HMO drying process (DP6”), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5) or (DP5’), wherein the drying gas is compressed air having a dew point of about -25° C.
  • the present invention relates to a HMO drying process (DP6’”), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5) or (DP5’), wherein the drying gas is nitrogen having a dew point of about -40° C.
  • the airflow rate (gas velocity) of the inlet gas in the process according to the present invention is usually and preferably 0.1-10 m/s.
  • the airflow rate is adapted depending on the powder density, the size and the shape of the material in the dryer.
  • the present invention relates to a HMO drying process (DP7), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”) or (DP6’”), wherein the airflow rate of the inlet gas is 0.1-10 m/s.
  • the duration of the drying process according to the present invention can vary. A usual duration is 0.5-8 h. It could be carried out longer, but from industrial and commercial purposes a longer duration is not useful.
  • the present invention relates to a HMO drying process (DP8), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”) or (DP7), wherein the duration of the drying process is 0.5-8 h.
  • the product parameters are those controlled by the product (the HMO which needs to dried).
  • Such product parameters are i.e. moisture content of the feed material (HMO), feed rate/batch size, product moisture, particle size of the feed material, particle shape of the feed material, and diameter of the feed material.
  • the moisture content of the feed material (HMO) is ⁇ 20%; more preferably ⁇ 10%, most preferably ⁇ 6%.
  • the present invention relates to a HMO drying process (DP9), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7) or (DP8), wherein the moisture content of the feed material (HMO) is ⁇ 20%.
  • DP9 drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7) or (DP8), wherein the moisture content of the feed material (HMO) is ⁇ 20%.
  • the present invention relates to a HMO drying process (DP9’), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7) or (DP8), wherein the moisture content of the feed material (HMO) is ⁇ 10%.
  • the present invention relates to a HMO drying process (DP9”), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7) or (DP8), wherein the moisture content of the feed material (HMO) is ⁇ 6%.
  • the drying process according to the present invention can be carried out continuously or batch-wise.
  • the present invention relates to a HMO drying process (DP10), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7), (DP8), (DP9), (DP9’) or (DP9”), wherein the drying process is carried out batch-wise.
  • the present invention relates to a HMO drying process (DP11), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7), (DP8), (DP9), (DP9’) or (DP9”), wherein the drying process is carried out continuously.
  • the batch size is not essential or critical.
  • the batch-size is de pendent on the size of the dryer which is used.
  • Usual batch -sizes are 0.5-5000kg.
  • the feed rate (for a continuous process) is in a range of .0.5- 10000kg/h.
  • the present invention relates to a HMO drying process (DP1T), which is drying process (DP11), wherein the feed rate is 0.5-1 OOOOkg/h.
  • Product moisture content is preferably ⁇ 1%.
  • the present invention relates to a HMO drying process (DP12), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DPT), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7), (DP8), (DP9), (DP9’), (DP9”), (DP10), (DP11) or (DP1 ), wherein the product moisture content (LOD) is ⁇ 1%.
  • the shape of the HMO, which is to be dried is not essential for the present invention.
  • the particle size (determined by the longest diameter of the particle) d50 (volumetric) is between 20 m ⁇ ti - 4000 pm, preferably 20 pm - 1000 pm.
  • All particle sizes of the solid particles according to the present invention are determined by laser diffraction technique using a “Mastersizer 3000” of Malvern instruments Ltd., UK Further information on this particle size characterization method can e.g. be found in “Basic principles of particle size analytics”, Dr Alan Rawle, Malvern Instruments Limited, Enigma Business Part, Grovewood Road, Malvern, Worcestershire, WR14 1XZ, UK and the “Manual of Malvern particle size analyzer”. Particular reference is made to the user manual number MAN 0098, Issue 1.0, November 1994.
  • d(v, 10) particle diameter corresponding to 10% of the cumulative under size distribution by volume
  • d(v, 50) particle diameter corresponding to 50% of the cumulative under size distribution by volume
  • d(v, 90) particle diameter corresponding to 90% of the cumulative under size distribution by volume
  • the present invention relates to a HMO drying process (DP13), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7), (DP8), (DP9), (DP9’), (DP9”), (DP10), (DP11), (DP1 T) or (DP12) wherein the particle size d50 (volumetric) is between 20 pm - 4000 pm.
  • the present invention relates to a HMO drying process (DP13’), which is drying process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7), (DP8), (DP9), (DP9’), (DP9”), (DP10), (DP11), (DP1 T) or (DP12) wherein the particle size d50 (volumetric) is between 20 pm - 1000 pm.
  • any commonly known and used fluidized bed dryer can be used.
  • fluidized bed dryers can be categorised in two categories
  • Conventional Fluidized Bed Dryers such as i.e. batch fluidized bed dryers; semi- continuous fluidized bed dryers; well-mixed, continuous fluidized dryers and plug flow fluidized bed dryer.
  • Modified Fluidized Bed Dryers such as i.e. Hybrid fluidized bed dryers; pulsating fluidized bed dryers; fluidized bed dryer with immersed heat exchange; mechanically assisted fluidized bed dryer; vibrated fluidized bed dryer; agitated fluidized bed dryer/swirl fluidizers; fluidized bed dryers of inert particles; spouted bed dryer; recirculating fluidized bed dryer; jetting fluidized bed dryer; superheated steam fluidized bed dryer; fluidized bed freeze dryer, fluidized bed spray dryer, heat pump fluidized dryers and fluidized bed mills.
  • the choice of the fluid bed dryer is not essential for the invention.
  • the present invention also relates to a HMO obtained by drying using process (DP), (DP1), (DPT), (DP1”), (DP2), (DP3), (DP4), (DP4’), (DP4”), (DP4’”), (DP5), (DP5’), (DP6), (DP6’), (DP6”), (DP6’”), (DP7), (DP8), (DP9), (DP9’), (DP9”), (DP10), (DP11), (DP1 T),(DP12), (DP13) or (DP13’).
  • the present invention also relates to a HMO having a water activity (a w ) of below 0.15, preferably below 0.12, more preferred below 0.1.
  • the present invention also relates to a HMO having a water activity (a w ) of below 0.15.
  • the present invention also relates to a HMO having a water activity (a w ) of below 0.12.
  • the present invention also relates to a HMO having a water activity (a w ) of below 0.1.
  • the present invention also relates to a HMO having a water activity (a w ) of 0.02 to 0.15.
  • the present invention also relates to a HMO having a water activity (a w ) of 0.02 to 0.12.
  • the present invention also relates to a HMO having a water activity (a w ) of 0.02 to 0.1.
  • the present invention also relates to a HMO having a water activity (a w ) of 0.05 to 0.15.
  • the present invention also relates to a HMO having a water activity (a w ) of 0.05 to 0.12.
  • the present invention also relates to a HMO having a water activity (a w ) of 0.05 to 0.10.
  • the HMOs with the a w as claimed above can be any HMO as defined above.
  • the most important ones are 2-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fu- cosyllactose (3FL), difucosyllactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllac- tose Sodium Salt (3'SL), 6 ' Sialyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT).
  • Especially important HMOs are 2'-fucosyllactose (2' FL) and lacto-N-neotetraose (LNnT).
  • the present invention also relates to a HMO chosen from the group consisting of 2 -fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl lactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 Si- alyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT) having a water activity (a w ) of below 0.15.
  • a HMO chosen from the group consisting of 2 -fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl lactose (DFL), Lacto-N-fucopentaose
  • the present invention also relates to a HMO chosen from the group consisting of 2 -fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl lactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 Si- alyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT) having a water activity (a w ) of below 0.12.
  • a HMO chosen from the group consisting of 2 -fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl lactose (DFL), Lacto-N-fucopentaose I
  • the present invention also relates to a HMO chosen from the group consisting of 2 -fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl lactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 Si- alyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT) having a water activity (a w ) of below 0.1.
  • a HMO chosen from the group consisting of 2 -fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl lactose (DFL), Lacto-N-fucopentaose
  • the present invention also relates to a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl lactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 Si- alyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT) having a water activity (a w ) of 0.02 to 0.15.
  • a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl lactose (DFL), Lacto-N-fucopentao
  • the present invention also relates to a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 Si- alyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT) having a water activity (a w ) of 0.02 to 0.15.
  • a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopent
  • the present invention also relates to a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 Si- alyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT) having a water activity (a w ) of 0.02 to 0.1.
  • a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopent
  • the present invention also relates to a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 Si- alyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT) having a water activity (a w ) of 0.05 to 0.15.
  • a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopent
  • the present invention also relates to a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 Si- alyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT) having a water activity (a w ) of 0.05 to 0.12.
  • a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopenta
  • the present invention also relates to a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopentaose I (LNFP I), 3 ' Sialyllactose Sodium Salt (3'SL), 6 Si- alyllactose Sodium Salt (6'SL), and Lacto-N-Tetraose (LNT) having a water activity (a w ) of 0.05 to 0.10.
  • a HMO chosen from the group consisting of 2'-fucosyllactose (2' FL), lacto-N-neotetraose (LNnT), 3-fucosyllactose (3FL), difucosyl- lactose (DFL), Lacto-N-fucopenta
  • 2’FL powder (Glycare 2’FL 9000) was is dried at 70°C in a nitrogen-operated fluidized bed dryer from DMR (type WFP-Mini ) for 2 hours. Drying air was heated to 70°C. After that, 500g of powder was added and fluidized via adding hot drying air at high pressure through a perforated bed . The powder particles are lifted from the bottom and air volume flow rate increased until a stable fluidized bed is generated. The integrated filters are holding back the powder particles, which are led back into the process by a permanent clean-off of the filters. After 2 hours, powder was discharged and cooled to room temperature in a desiccator.
  • the a w before the drying was 0.25 (measured at 25 °C using a Novasina LabMaster).
  • the a w after the drying was 0.14 (measured at 25 °C using a Novasina LabMaster).
  • the a w after 4 weeks was 0.14 (measured at 25 °C using a Novasina LabMaster).

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Abstract

La présente invention concerne un nouveau procédé de séchage d'oligosaccharides du lait maternel (HMO). Ce procédé permet d'obtenir des HMO ayant une très faible activité de l'eau.
PCT/EP2022/060470 2021-04-22 2022-04-21 Procédé de séchage pour hmos WO2022223652A1 (fr)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2116137A2 (fr) * 2008-05-07 2009-11-11 Brunob Ii B.V. Polysaccharides inhibés thermiquement et procédé de préparation
WO2011150939A1 (fr) * 2010-06-01 2011-12-08 Glycom A/S Formes polymorphiques du 2'-o-fucosyllactose et leurs procédés de production
WO2016138911A1 (fr) * 2015-03-05 2016-09-09 Glycom A/S Composition et méthode pour le traitement d'infections aiguës des voies respiratoires
WO2017060477A1 (fr) * 2015-10-07 2017-04-13 Bifodan A/S Formulation de probiotiques
EP3494805A1 (fr) * 2017-12-08 2019-06-12 Jennewein Biotechnologie GmbH Tétrasaccharide séché en pulvérisation
WO2019160922A1 (fr) * 2018-02-19 2019-08-22 Dupont Nutrition Biosciences Aps Procédé de séchage par pulvérisation de solutions de fucosyllactose et compositions de produit associées

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2116137A2 (fr) * 2008-05-07 2009-11-11 Brunob Ii B.V. Polysaccharides inhibés thermiquement et procédé de préparation
WO2011150939A1 (fr) * 2010-06-01 2011-12-08 Glycom A/S Formes polymorphiques du 2'-o-fucosyllactose et leurs procédés de production
WO2016138911A1 (fr) * 2015-03-05 2016-09-09 Glycom A/S Composition et méthode pour le traitement d'infections aiguës des voies respiratoires
WO2017060477A1 (fr) * 2015-10-07 2017-04-13 Bifodan A/S Formulation de probiotiques
EP3494805A1 (fr) * 2017-12-08 2019-06-12 Jennewein Biotechnologie GmbH Tétrasaccharide séché en pulvérisation
WO2019160922A1 (fr) * 2018-02-19 2019-08-22 Dupont Nutrition Biosciences Aps Procédé de séchage par pulvérisation de solutions de fucosyllactose et compositions de produit associées

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AGOSTINO CARLO: "Safety of 2'-O-fucosyllactose as a novel food ingredient pursuant to Regulation (EC) No 258/97", vol. 13, no. 7, 1 July 2015 (2015-07-01), Parma, IT, pages 4184, XP055844929, ISSN: 1831-4732, Retrieved from the Internet <URL:https://www.bfr.bund.de/cm/343/efsa-opinion-on-the-safety-of-2-o-fucosyllactose.pdf> [retrieved on 20210927], DOI: 10.2903/j.efsa.2015.4184 *
CHUNG LIM LAW ET AL: "Fluidized bed dryers", 30 November 2006, HANDBOOK OF INDUSTRIAL DRYING, CRC, TAYLOR & FRANCIS, BOCA RATON, PAGE(S) 173 - 201, ISBN: 978-1-57444-668-5, XP009522276 *

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