WO2022213977A1 - 混匀管 - Google Patents

混匀管 Download PDF

Info

Publication number
WO2022213977A1
WO2022213977A1 PCT/CN2022/085299 CN2022085299W WO2022213977A1 WO 2022213977 A1 WO2022213977 A1 WO 2022213977A1 CN 2022085299 W CN2022085299 W CN 2022085299W WO 2022213977 A1 WO2022213977 A1 WO 2022213977A1
Authority
WO
WIPO (PCT)
Prior art keywords
stirring member
stirring
width
mixing
mixing tube
Prior art date
Application number
PCT/CN2022/085299
Other languages
English (en)
French (fr)
Inventor
张昊
孙立伟
魏金文
乔月华
房瑜
孙振波
张准
Original Assignee
南京金斯瑞生物科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 南京金斯瑞生物科技有限公司 filed Critical 南京金斯瑞生物科技有限公司
Priority to CN202280025955.5A priority Critical patent/CN117098979A/zh
Publication of WO2022213977A1 publication Critical patent/WO2022213977A1/zh
Priority to US18/465,964 priority patent/US20230415109A1/en

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/40—Parts or components, e.g. receptacles, feeding or discharging means
    • B01F29/401—Receptacles, e.g. provided with liners
    • B01F29/402—Receptacles, e.g. provided with liners characterised by the relative disposition or configuration of the interior of the receptacles
    • B01F29/4022—Configuration of the interior
    • B01F29/40221—Configuration of the interior provided with baffles, plates or bars on the wall or the bottom
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/15—Use of centrifuges for mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/50—Mixing receptacles
    • B01F35/53—Mixing receptacles characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components
    • B01F35/531—Mixing receptacles characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components with baffles, plates or bars on the wall or the bottom
    • B01F35/5312—Mixing receptacles characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components with baffles, plates or bars on the wall or the bottom with vertical baffles mounted on the walls
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation

Definitions

  • the present application relates to the field of substance mixing, in particular to a mixing tube.
  • Some embodiments of this specification provide a mixing tube, the mixing tube includes: a tube body, the tube body is used for accommodating a sample; a first stirring member and a second stirring member disposed on the inner wall of the tube body The ratio of the width to the thickness of the first stirring member is greater than the ratio of the width to the thickness of the second stirring member.
  • the ratio of the width to the thickness of the first stirring member is greater than 3.
  • the width of the first stirring member is in the range of 3.5 mm to 5 mm, and the thickness of the first stirring member is in the range of 1 mm to 1.2 mm.
  • the ratio of the width to the thickness of the second stirring member is less than 1.5.
  • the width of the second stirring member is in the range of 1.6 mm ⁇ 1.9 mm, and the thickness of the second stirring member is in the range of 1.2 mm ⁇ 1.4 mm.
  • the height of the first stirring member is in the range of 5mm ⁇ 100mm.
  • the height of the second stirring member is in the range of 10mm ⁇ 100mm.
  • a gap is provided between the first stirring member and the inner wall of the pipe body in the width direction.
  • the length of the gap is no greater than 10 mm.
  • the inner wall of the pipe body includes an inner side wall and an inner bottom wall closed and connected to one end of the inner side wall; the first stirring member is disposed on the inner bottom wall, and the second stirring member arranged on the inner side wall.
  • the bottom of the inner bottom wall is a flat surface or an upper convex surface, and the upper convex surface protrudes toward the interior of the tube body.
  • the bottom of the inner bottom wall is provided with a convex portion protruding toward the inside of the pipe body.
  • the number of the first stirring member and the number of the second stirring member is two.
  • the two first stirring members and the two second stirring members are symmetrically disposed relative to the central axis of the pipe body.
  • the angle formed by the connecting line between the two first stirring members and the connecting line between the two second stirring members is 90 degrees.
  • first stirring member and the second stirring member are arranged circumferentially spaced along the inner wall of the pipe body.
  • the first stirring member includes a first end and a second end, the first end is connected to the inner wall of the pipe body, and the second end extends toward the center of the pipe body.
  • Fig. 1 is a partial structural schematic diagram of a pipe body according to some embodiments of the present application.
  • FIG. 2 is a schematic structural diagram of a mixing pipe according to some embodiments of the present application.
  • Fig. 3 is the top view of Fig. 2;
  • Fig. 4 is a partial structural schematic diagram of a pipe body according to other embodiments of the present application.
  • Fig. 5 is a partial structural schematic diagram of a pipe body according to further embodiments of the present application.
  • FIG. 6 is a perspective view of the structure of a mixing pipe according to other embodiments of the present application.
  • FIG. 7 is a schematic structural diagram of a mixing pipe according to other embodiments of the present application.
  • Fig. 8 is a partial structural schematic diagram of a pipe body according to further embodiments of the present application.
  • FIG. 9 is a schematic cross-sectional view of a mixing pipe according to some embodiments of the present application.
  • FIG. 10 is a schematic cross-sectional view of a mixing pipe according to other embodiments of the present application.
  • Reference numerals mixing pipe 10; pipe body 20; inner wall 200; inner side wall 210; inner bottom wall 220; raised part 221; first stirring member 30; second stirring member 40; gap 50; first anti-rotation part 60; the second anti-rotation part 70; the thread 80.
  • the term “based on” is “based at least in part on.”
  • the term “one embodiment” means “at least one embodiment”; the term “another embodiment” means “at least one additional embodiment”; the term “within” means a numerical range inclusive of both endpoints, such as In the range of 1.5mm-15mm, in addition to the values between 1.5mm-15mm, the indicated range also includes 1.5mm and 15mm. Relevant definitions of other terms will be given in the description below.
  • the step of mixing the two or more substances may include placing the two or more substances (ie, samples) into a mixing tube, and then rotating or manipulating using an external device (eg, a mixing device) Personnel manually rotate the mixing tube to make two or more substances in the mixing tube perform centrifugal motion, thereby achieving the purpose of mixing.
  • an external device eg, a mixing device
  • each substance is different, when two or more substances are accommodated in the mixing pipe, they will be in different areas in the mixing pipe (for example, a solid material with a larger specific gravity is mainly accumulated in the mixing pipe). At the bottom of the mixing tube, a liquid substance with a small specific gravity is mainly located in other areas of the mixing tube except the bottom).
  • an auxiliary stirring structure can be arranged in the mixing tube to help improve the mixing effect.
  • the auxiliary stirring structure may include stirring ribs disposed on the inner wall of the mixing pipe. The use of stirring bars can make the liquid material in the mixing tube generate vortex during the mixing process, and the vortex introduces the liquid material into the area where the solid material is located to realize mixing.
  • the auxiliary stirring structure may include stirring blades disposed on the inner wall of the mixing tube. The stirring blade can be used to impact the substances to be mixed during the mixing process to break up the accumulated substances, so as to achieve full mixing.
  • the auxiliary stirring structure may simultaneously include stirring blades and stirring ribs disposed on the inner wall of the mixing tube.
  • the stirring blade to disperse the accumulated substances, and use the stirring ribs to generate vortex, so as to achieve more sufficient mixing and improve the mixing effect.
  • the ratio of the width to the thickness of the stirring blade is greater than the ratio of the width to the thickness of the stirring rib compared with the stirring rib. It can also be said that the stirring blade is thinner than the stirring rib.
  • the width of the stirring blade is greater than the width of the stirring rib compared with the stirring rib, and it can also be said that the stirring blade is wider than the stirring rib.
  • the stirring ribs and the stirring blades can impact and stir the substances in different areas in the tube body respectively, so that the substances move more violently, so that the mixing between substances is more sufficient and improves the mixed effect.
  • the mixing tube will be exemplarily described below with reference to the accompanying drawings.
  • the mixing tube 10 may include a tube body 20 for containing a sample (not shown in the figure).
  • the inner wall 200 of the pipe body 20 is provided with a first stirring member 30 and a second stirring member 40 .
  • the ratio of the width to the thickness of the first stirring member 30 is greater than the ratio of the width to the thickness of the second stirring member 40, so that the sample can be mixed and stirred more fully, and the mixing effect can be improved.
  • the width of the first stirring member 30 can be made larger than the width of the second stirring member 40, and the above effects can also be achieved.
  • the width of the stirring member refers to the size of the stirring member in the direction extending from the inner wall of the tube body 20 to the inside of the tube body 20 .
  • the width X1 of the first stirring member 30 and the width X2 of the second stirring member 40 in FIG. 3 are shown.
  • the thickness of the stirring member refers to the distance between the two side surfaces of the stirring member extending from the inner wall of the tube body 20 to the inside of the tube body 20 .
  • the thickness Y1 of the first stirring member 30 in FIG. 3 is the thickness Y2 of the second stirring member 40 .
  • the ratio of the width X1 to the thickness Y1 of the first stirring member 30 is greater than the ratio of the width X2 to the thickness Y2 of the second stirring member 40, so the first stirring member 30 is thinner than the second stirring member 40, so , the first stirring piece 30 can also be called a stirring piece, and the second stirring piece 40 can also be called a stirring rib.
  • the mixing tube 10 in this application can be used as a container for sample mixing.
  • sample mixing can be understood as mixing two or more substances contained in the sample.
  • the mixing of two or more substances may include mixing of solid substances and liquid substances, and mixing of liquid substances and liquid substances, and the like.
  • an E. coli slurry ie, a solid substance obtained by centrifuging an E. coli culture solution
  • cell resuspension a cell resuspension.
  • this application uses the mixing of Escherichia coli bacteria puree and cell resuspension as an example for description.
  • the mixing tube 10 can be used as a storage container to store samples.
  • the mixing tube 10 can be used as a container for centrifugation, and cooperates with a centrifugal device to perform centrifugal separation of substances with different specific gravities in the samples stored in the mixing tube 10 .
  • the mixing tube 10 when used as a container for centrifugation, it may be referred to as a centrifuge tube.
  • the mixing tube 10 can be used as a reaction vessel, and several samples stored in the mixing tube 10 can react.
  • the mixing tube 10 can be used as a container for centrifugation and sample mixing at the same time (for example, in the mixing tube 10, the E. coli culture solution is centrifuged to obtain E. coli bacteria slurry, and then the E. coli bacteria slurry and cells are resuspended. liquid mixing).
  • the sample may include two or more substances to be mixed, for example, the sample may include E. coli slurry and cell resuspension.
  • the first stirring member 30 and the second stirring member 40 may be configured to impact and stir the sample when the sample in the tube body 20 moves under the action of inertia and centrifugal force. Shock and agitation can cause more vigorous movement of the substances in the sample, speed up the mixing rate, and improve the mixing effect between substances.
  • the ratio of the width X1 to the thickness Y1 of the first stirring member 30 is greater than 3. In some embodiments, the ratio of the width X1 to the thickness Y1 of the first stirring member 30 ranges from 3 to 20. In some embodiments, the ratio of the width X1 to the thickness Y1 of the first stirring member 30 ranges from 3 to 15. In some embodiments, the ratio of the width X1 to the thickness Y1 of the first stirring member 30 ranges from 3 to 10. In some embodiments, the ratio of the width X1 to the thickness Y1 of the first stirring member 30 ranges from 3 to 4.
  • the width X1 of the first stirring member 30 may be in the range of 1.5 mm ⁇ 15 mm, and the thickness Y1 may be in the range of 0.5 mm ⁇ 3 mm. In some embodiments, the width X1 of the first stirring member 30 may be in the range of 2.5 mm ⁇ 10 mm, and the thickness Y1 may be in the range of 0.75 mm ⁇ 2 mm. In some embodiments, the width X1 of the first stirring member 30 may be in the range of 3.5 mm ⁇ 5 mm, and the thickness Y1 may be in the range of 1 mm ⁇ 1.5 mm. Preferably, in some embodiments, the width X1 of the first stirring member 30 may be 3.5 mm, and the thickness Y1 of the first stirring member 30 may be in the range of 1 mm ⁇ 1.2 mm.
  • the ratio of the width X2 to the thickness Y2 of the second stirring member 40 is less than 1.5. In some embodiments, the ratio of the width X2 to the thickness Y2 of the second stirring member 40 ranges from 0.1 to 1.5. In some embodiments, the ratio of the width X2 to the thickness Y2 of the second stirring member 40 ranges from 0.5 to 1.5. In some embodiments, the ratio of the width X2 to the thickness Y2 of the second stirring member 40 ranges from 0.75 to 1.5. In some embodiments, the ratio of the width X2 to the thickness Y2 of the second stirring member 40 ranges from 1 to 1.5.
  • the width X2 of the second stirring member 40 may be in the range of 1 mm ⁇ 3 mm, and the thickness Y2 of the second stirring member 40 may be in the range of 0.6 mm ⁇ 2 mm. In some embodiments, the width X2 of the second stirring member 40 may be in the range of 1.2 mm ⁇ 2.5 mm, and the thickness Y2 of the second stirring member 40 may be in the range of 0.8 mm ⁇ 1.8 mm. In some embodiments, the width X2 of the second stirring member 40 may be in the range of 1.4 mm ⁇ 2 mm, and the thickness Y2 of the second stirring member 40 may be in the range of 1 mm ⁇ 1.6 mm.
  • the width X2 of the second stirring member 40 may be in the range of 1.6 mm ⁇ 1.9 mm, and the thickness Y2 of the second stirring member 40 may be in the range of 1.2 mm ⁇ 1.4 mm. In some preferred embodiments, the width X2 of the second stirring member 40 may be 1.8 mm, and the thickness Y2 of the second stirring member 40 may be in the range of 1.2 mm ⁇ 1.4 mm.
  • the cross-sectional shape of the stirring rib can be in various forms, including but not limited to triangular-like (that is, one side of the triangle is an arc), trapezoid-like (that is, one side of the trapezoid is an arc), or rectangle-like (that is, a rectangle). one side is an arc).
  • the difference between the ratio of the width X1 to the thickness Y1 of the first stirring member 30 and the ratio of the width X2 to the thickness Y2 of the second stirring member 40 is in the range of 0.5 ⁇ 5. In some embodiments, the difference between the ratio of the width X1 to the thickness Y1 of the first stirring member 30 and the ratio of the width X2 to the thickness Y2 of the second stirring member 40 is in the range of 1-4. In some embodiments, the difference between the ratio of the width X1 to the thickness Y1 of the first stirring member 30 and the ratio of the width X2 to the thickness Y2 of the second stirring member 40 is in the range of 1.5-3. In some preferred embodiments, the difference between the ratio of the width X1 to the thickness Y1 of the first stirring member 30 and the ratio of the width X2 to the thickness Y2 of the second stirring member 40 is in the range of 1.5-2.
  • the ratio of the ratio of the width X1 to the thickness Y1 of the first stirring member 30 to the ratio of the width X2 to the thickness Y2 of the second stirring member 40 is in the range of 1-5. In some embodiments, the ratio of the ratio of the width X1 to the thickness Y1 of the first stirring member 30 to the ratio of the width X2 to the thickness Y2 of the second stirring member 40 is in the range of 1.5-4. Preferably, in some embodiments, the ratio of the ratio of the width X1 to the thickness Y1 of the first stirring member 30 to the ratio of the width X2 to the thickness Y2 of the second stirring member 40 is in the range of 2-3.
  • the width X1 of the first stirring member 30 may be greater than the width X2 of the second stirring member 40 to improve the mixing effect.
  • the width X1 of the first stirring member 30 may be in the range of 1.5mm ⁇ 15mm. In some embodiments, the width X1 of the first stirring member 30 may be in the range of 2.5mm ⁇ 10mm. In some embodiments, the width X1 of the first stirring member 30 may be in the range of 3.5mm ⁇ 5mm. Preferably, in some embodiments, the width X1 of the first stirring member 30 may be 3.5 mm.
  • the width X2 of the second stirring member 40 may be in the range of 1 mm ⁇ 3 mm. In some embodiments, the width X2 of the second stirring member 40 may be in the range of 1.2 mm ⁇ 2.5 mm. In some embodiments, the width X2 of the second stirring member 40 may be in the range of 1.4 mm ⁇ 2 mm. In some embodiments, the width X2 of the second stirring member 40 is in the range of 1.6 mm ⁇ 1.8 mm. Preferably, in some embodiments, the width X2 of the second stirring member 40 may be 1.8 mm.
  • the difference between the width X1 of the first stirring member 30 and the width X2 of the second stirring member 40 may be in the range of 0.5 mm ⁇ 14 mm. In some embodiments, the difference between the width X1 of the first stirring member 30 and the width X2 of the second stirring member 40 may be in the range of 1 mm ⁇ 10 mm. In some embodiments, the difference between the width X1 of the first stirring member 30 and the width X2 of the second stirring member 40 may be in the range of 1.25 mm ⁇ 5 mm. In some embodiments, the difference between the width X1 of the first stirring member 30 and the width X2 of the second stirring member 40 may be in the range of 1.5 mm ⁇ 2 mm. Preferably, in some embodiments, the difference between the width X1 of the first stirring member 30 and the width X2 of the second stirring member 40 may be 1.7 mm.
  • the ratio of the width X1 of the first stirring member 30 to the width X2 of the second stirring member 40 is in the range of 1 ⁇ 5. In some embodiments, the ratio of the width X1 of the first stirring member 30 to the width X2 of the second stirring member 40 is in the range of 1.25-3. In some embodiments, the ratio of the width X1 of the first stirring member 30 to the width X2 of the second stirring member 40 is in the range of 1.5 ⁇ 2.5. Preferably, in some embodiments, the ratio of the width X1 of the first stirring member 30 to the width X2 of the second stirring member 40 is in the range of 1.75-2.
  • the heights of the first stirring member 30 and the second stirring member 40 are related to the amount of the sample to be mixed and the total length S1 of the tube body 20 (as shown in FIG. 10 ).
  • the height of the stirring member may refer to the dimension in the direction in which the stirring member extends near the bottom of the tube body 20 and extends away from the top of the tube body 20 .
  • the height Z1 of the first stirring member 30 and the height Z2 of the second stirring member 40 are shown.
  • the height Z1 of the first stirring member 30 may be in the range of 5 mm ⁇ 100 mm. In some embodiments, the height Z1 of the first stirring member 30 may be in the range of 10mm ⁇ 75mm. In some embodiments, the height Z1 of the first stirring member 30 may be in the range of 15mm ⁇ 50mm. Preferably, in some embodiments, the height Z1 of the first stirring member 30 may be 15 mm. In some embodiments, the height Z2 of the second stirring member 40 may be in the range of 10mm ⁇ 100mm. In some embodiments, the height Z2 of the second stirring member 40 may be in the range of 30mm ⁇ 90mm.
  • the height Z2 of the second stirring member 40 may be in the range of 50mm ⁇ 80mm. In some embodiments, the height Z2 of the second stirring member 40 may be in the range of 60mm ⁇ 70mm. Preferably, in some embodiments, the height Z2 of the second stirring member 40 may be 60 mm.
  • the inner wall 200 of the pipe body 20 may include an inner side wall 210 and an inner bottom wall 220 closed and connected to one end of the inner side wall 210; the first stirring member 30 may be provided On the inner bottom wall 220 , the second stirring member 40 may be disposed on the inner side wall 210 .
  • the first stirring member 30 and the second stirring member 40 can impact and stir the substances in different regions of the mixing tube 10 respectively, so as to make the sample move more violently. Still taking the samples including E. coli sludge and cell resuspension as an example, the E.
  • coli bacteria sludge is mainly accumulated in the bottom area of the mixing tube 10, that is, at the inner bottom wall 220 due to its large specific gravity; while the cell resuspension has a small specific gravity. , it will be mainly located in the area of the mixing tube 10 other than the bottom, for example, the area corresponding to the inner side wall 210 .
  • the mixing tube 10 rotates, the E. coli bacteria sludge will move under the action of centrifugal force and inertia, and the first stirring member 30 will impact and agitate the E. coli bacteria sludge to make it move more violently.
  • the cell resuspension will also move under the action of centrifugal force and inertia, and the second stirring member 40 will impact and stir the cell resuspension, so that the cell resuspension will vibrate to generate vortex and turbulent flow, thereby making the cell resuspension liquid. Shock the E. coli mash for thorough mixing.
  • first stirring member 30 and the second stirring member 40 may be circumferentially disposed along the inner wall 200 of the pipe body 20. In some embodiments, the first stirring member 30 and the second stirring member 40 may be arranged at intervals. In this embodiment, the first stirring member 30 and the second stirring member 40 are arranged circumferentially spaced along the inner wall 200 of the tube body 20 to effectively improve the mixing effect of two or more substances in the sample.
  • the mixing tube 10 can be used in conjunction with an external device (eg, a mixing device) to improve the mixing effect of the sample.
  • an external device eg, a mixing device
  • Escherichia coli bacteria sludge is mainly accumulated on one side of the inner wall 200 . If there is no gap 50 between the first stirring member 30 and the inner wall 200 , a dead corner will be formed, and Escherichia coli bacteria sludge will accumulate in the dead corner and cannot be sufficiently mixed with the cell resuspension.
  • the first stirring member 30 may have a gap 50 with the inner wall 200 of the pipe body 20 in the width direction. 1 and 4, due to the existence of the gap 50, there is no dead angle between the first stirring member 30 and the inner wall 200, which can effectively avoid the accumulation of Escherichia coli bacteria sludge and improve the mixing effect.
  • the length H of the gap 50 between the first stirring member 30 and the inner wall 200 of the pipe body 20 in the width direction may not be greater than 10 mm. In some embodiments, the length H of the gap 50 between the first stirring member 30 and the inner wall 200 of the pipe body 20 in the width direction may be in the range of 1 mm ⁇ 6 mm. In some embodiments, the length H of the gap 50 between the first stirring member 30 and the inner wall 200 of the pipe body 20 in the width direction may be in the range of 1.5 mm ⁇ 3 mm. In some preferred embodiments, the length H of the gap 50 between the first stirring member 30 and the inner wall 200 of the pipe body 20 in the width direction may be 2 mm.
  • the first stirring member 30 may include a first end and a second end, the first end may be connected to the inner wall 200 of the pipe body 20, and the second end is along the central axis O of the pipe body 20 (as shown in FIG. 9 ). and in the direction parallel to that shown in Figure 10).
  • the second end of the first stirring member 30 may extend toward the center of the pipe body 20 , that is, the extending direction of the first stirring member 30 is in the direction of the central axis O of the pipe body 20 At a certain angle, to further improve the mixing effect.
  • the first end of the first stirring member 30 may be connected to the inner bottom wall 220 of the pipe body 20.
  • the ends are attached to the inner bottom wall 220 .
  • the first end of the first stirring member 30 may be connected to an end of the inner side wall 210 of the pipe body 20 close to the inner bottom wall 220 .
  • the distance S2 between the first end of the first stirring member 30 and the bottom of the inner bottom wall 220 may be It is 1/7-1/3 of the total length S1 of the pipe body 20 .
  • the distance S2 between the first end of the first stirring member 30 and the bottom of the inner bottom wall 220 may be 1/6 ⁇ 1/3 of the total length S1 of the pipe body 20 .
  • the distance S2 between the first end of the first stirring member 30 and the bottom of the inner bottom wall 220 may be 1/5 ⁇ 1/3 of the total length S1 of the pipe body 20 .
  • the first stirring member 30 can also be configured to be connected to the tube body in its width direction.
  • the inner bottom walls 220 of 20 are connected smoothly, that is, the connection in the width direction is provided with rounded corners.
  • the first stirring member 30 and the second stirring member 40 can impact the sample, thereby causing the sample to move more violently and improving the mixing effect between substances.
  • the arrangement positions of the first stirring member 30 and the second stirring member 40 and the influence of the specific structures on the mixing effect are described.
  • the number of the first stirring member 30 and the second stirring member 40 also affects the mixing effect between the substances.
  • the number of the first stirring member 30 and the number of the second stirring member 40 may be two.
  • the liquid eg, cell resuspension
  • the E. coli bacteria slurry will also be impacted and agitated by the two first stirring members 30, and the resulting movement will be more intense. Therefore, the mixing of the E. coli bacteria slurry and the cell resuspension will be more complete, and the mixing rate will be faster. quick.
  • the numbers of the first stirring members 30 and the second stirring members 40 are not limited to two, but may also be one, three, four or more.
  • the number of the first stirring member 30 and the number of the second stirring member 40 is one.
  • the number of the first stirring members 30 and the second stirring members 40 may be the same.
  • the number of the first stirring member 30 and the number of the second stirring member 40 may be two, as shown in FIG. 3 and FIG. 6 .
  • the number of the first stirring member 30 and the number of the second stirring member 40 may be one.
  • the numbers of the first stirring members 30 and the second stirring members 40 may be different.
  • the number of the first stirring members 30 is one, and the number of the second stirring members 40 is two.
  • the number of the first stirring members 30 is two, and the number of the second stirring members 40 is four.
  • the arrangement of the first stirring members 30 and the second stirring members 40 may be related to the number of the first stirring members 30 and the second stirring members 40 .
  • the two first stirring members 30 and the two second stirring members 40 may be symmetrically disposed relative to the central axis O of the pipe body 20 .
  • the two second stirring members 40 can simultaneously perform impact stirring on the cell resuspension, and at the same time, the two first stirring members 30 can also perform impact stirring on the Escherichia coli bacteria sludge, so that the The cell resuspension and E. coli slurry can be mixed more uniformly, improving the mixing effect of the sample.
  • the eddy current and the turbulent current generated when each second stirring member 40 impacts the cell resuspension can not affect each other, so as to further improve the mixing effect.
  • the three first stirring members 30 may be arranged around the inner bottom wall 220 at the same interval, that is, two adjacent first stirring members 30
  • the included angle formed by the connection line between the stirring member 30 and the central axis O of the pipe body 20 is 120 degrees.
  • the four first stirring members can also be arranged around the inner bottom wall 220 at the same interval, that is, two adjacent first stirring members 30 and The included angle formed by the connecting line of the central axis O of the pipe body 20 is 90 degrees.
  • the second stirring member 40 reference may also be made to the description of the embodiment of the first stirring member 30.
  • the three second stirring members 40 can be the same The spacers are arranged around the inner side wall 210 , and the included angle formed by the connection line between the two adjacent second stirring members 40 and the central axis O of the pipe body 20 is 120 degrees.
  • the number of the second stirring members 40 is four, the four second stirring members 40 may be arranged around the inner side wall 210 at the same interval, and the two adjacent second stirring members 40 and the central axis of the pipe body 20 The angle formed by the connecting lines of O is 90 degrees.
  • the present embodiment is only used as an example to illustrate the arrangement of the first stirring member 30 and the second stirring member 40.
  • the first stirring member 30 and the second stirring member can be 40's settings have been improved.
  • the two first stirring members 30 may be symmetrically arranged relative to the central axis O of the tube body 20
  • the second stirring members 40 are not symmetrical relative to the central axis O of the tube body 20 .
  • the number of the first stirring members 30 is four
  • the number of the second stirring members 40 is three
  • the three second stirring members 40 may be arranged around the inner side wall 210 at the same interval
  • the pieces 30 may be disposed around the inner bottom wall 220 at various intervals.
  • FIG. 3 exemplarily shows an embodiment in which the angle ⁇ formed by the connecting line between the two first stirring members 30 and the connecting line between the two second stirring members 40 is 90 degrees.
  • the mixing effect of the sample is also related to the clip formed by the connecting line between the two first stirring members 30 and the connecting line between the two second stirring members 40 .
  • the angle of the angle is related.
  • the angle range of the included angle ⁇ formed by the connecting line between the two first stirring members 30 and the connecting line between the two second stirring members 40 includes 30 degrees to 90 degrees.
  • the angle range of the included angle ⁇ formed by the connecting line between the two first stirring members 30 and the connecting line between the two second stirring members 40 includes 45 degrees to 90 degrees.
  • the angle range of the included angle ⁇ formed by the connecting line between the two first stirring members 30 and the connecting line between the two second stirring members 40 includes 60 degrees to 90 degrees. In some preferred embodiments, the angle ⁇ formed by the connecting line between the two first stirring members 30 and the connecting line between the two second stirring members 40 is 90 degrees.
  • the three first stirring members 30 may be arranged around the inner bottom wall 220 at the same interval, and the three second stirring members The members 40 can be arranged around the inner side wall 210 at the same interval, and the included angle between the line connecting the first stirring member 30 and the central axis O of the pipe body 20 and the connecting line between the second stirring member 40 and the central axis O of the pipe body 20 can be 60 degrees.
  • the four first stirring members 30 may be arranged around the inner bottom wall 220 at the same interval, and the four second stirring members The members 40 can be arranged around the inner side wall 210 at the same interval, and the included angle between the line connecting the first stirring member 30 and the central axis O of the pipe body 20 and the connecting line between the second stirring member 40 and the central axis O of the pipe body 20 can be 45 degree.
  • the number of the first stirring members 30 is different from the number of the second stirring members 40, for example, when the number of the first stirring members 30 is four and the number of the second stirring members 40 is two,
  • the two first stirring members 30 can be symmetrically arranged on the inner bottom wall 220 relative to the central axis O of the pipe body 20 , and the four second stirring members 40 can be arranged around the inner side wall 210 at the same interval.
  • the included angle between the line connecting the central axis O of the body 20 and the line connecting the second stirring member 40 and the central axis O of the pipe body 20 may be 30 degrees.
  • the two second stirring members 40 may be symmetrically arranged on the inner side wall 210 relative to the central axis O of the pipe body 20,
  • the included angle formed by the connecting line between the one stirring member 30 and the central axis O of the pipe body 20 and the connecting line between the two second stirring members 40 may be 90 degrees.
  • the material of the first stirring member 30 and the second stirring member 40 may be the same as the material of the pipe body 20, including polyethylene, polycarbonate, polypropylene, and the like.
  • the first stirring member 30 , the second stirring member 40 and the pipe body 20 may be integrally formed, or may be separately formed and then assembled.
  • the mixing tube 10 may only include the first stirring member 30, and the first stirring member 30 may be disposed along the inner wall 200 of the tube body 20 in the circumferential direction. The purpose of the blending effect.
  • the mixing tube 10 may only include the first stirring member 30 , the first stirring member 30 may be disposed along the circumference of the inner wall 200 of the tube body 20 , and the first stirring member 30 may be connected to the tube body in the width direction.
  • the inner wall 200 of 20 is provided with a gap 50 .
  • the number of the first stirring member 30 may be one, two, three or more.
  • the width, thickness, ratio of width to thickness, height, and the setting position of the first stirring member 30 of the first stirring member 30 can be referred to in this specification.
  • the description of the first stirring member 30 in other embodiments is not repeated here.
  • the mixing between substances mainly relies on the centrifugal force generated when the mixing tube 10 performs the reciprocating rotation motion and the inertia of the sample, so that the substances move, and are further impacted by the first stirring member 30 and the second stirring member 40, Stir to mix.
  • the inner bottom wall 220 of the tube body 20 may protrude outward in a direction away from the tube body 20 to form a cone. The bacillus sludge may easily accumulate at the bottom of the inner bottom wall 220 and cannot be sufficiently mixed with the cell resuspension, reducing the mixing effect.
  • the bottom of the inner bottom wall 220 can be designed.
  • the inner bottom wall 220 can be set to protrude outwardly away from the direction of the pipe body 20 to form a cone, and the angle of the apex angle of the cone in the plane where the generatrix is located is greater than 90 degrees.
  • the bottom of the inner bottom wall 220 can be set to be flat, that is, the tube body 20 has a flat bottom, as shown in FIG. 9 and FIG. 10 . When the tube body 20 has a flat bottom, the accumulation of Escherichia coli bacteria sludge at the bottom can be effectively reduced.
  • the bottom of the inner bottom wall 220 may be set as an upper convex surface, and the so-called upper convex surface may mean that the bottom of the inner bottom wall 220 protrudes toward the inside of the tube body 20 . Since the bottom of the inner bottom wall 220 protrudes upward (ie, the inside of the tube body 20 ), even if the centrifugal force and inertial force of the E. coli bacteria sludge are relatively small, it cannot accumulate on the upper convex surface.
  • the bottom of the inner bottom wall 220 can also be provided with a protruding portion 221 protruding toward the interior of the tube body 20 to prevent E. coli bacteria sludge accumulation to improve the mixing effect.
  • the shape of the raised portion 221 may be a cone, a cylinder, a hemisphere, a semi-ellipsoid, etc., or a combination thereof.
  • the raised portion 221 may be a cone.
  • the protruding portion 221 may be a hemisphere.
  • connection between the edge of the raised portion 221 and the inner bottom wall 220 may be a smooth connection, that is, the connection is an arc surface, so as to avoid the accumulation of Escherichia coli bacteria sludge between the raised portion 221 and the inner bottom wall 220 connection to improve the blending effect.
  • the raised portion 221 may be combined with the structure related to the bottom of the inner bottom wall 220 in one or more of the foregoing embodiments.
  • the bottom of the inner bottom wall 220 may be flat, and at the same time, the bottom of the inner bottom wall 220 is further provided with a protruding portion 221 protruding toward the inside of the tube body 20 .
  • the outer wall of the pipe body 20 may be provided with an anti-rotation part.
  • the anti-rotation part can be used to prevent the mixing pipe 10 from rotating. Relative movement occurs after mating with an external device.
  • the external device here varies according to the purpose of the mixing tube 10.
  • the external device may be a mixing device.
  • the external device may be a centrifugal device.
  • the anti-rotation part may include a first anti-rotation part 60 disposed at one end of the outer wall of the pipe body 20 away from the inner bottom wall 220 .
  • the first anti-rotation portion 60 may be a flange surrounding the outer wall of the pipe body 20 (as shown in FIG. 4 ). When the mixing pipe 10 is matched with the external device, the flange can also be matched with the external device, so as to ensure that the mixing pipe 10 will not be disengaged when the mixing pipe 10 is rotated under the driving of the external device.
  • the anti-rotation part may further include a second anti-rotation part 70 disposed on the outer side wall of the pipe body 20 .
  • the second anti-rotation part 70 may be a groove provided along the axis direction of the pipe body 20 .
  • the groove can be engaged with the snap fit of the external device, thereby ensuring the relative fixation of the mixing tube 10 and the external device.
  • the second anti-rotation part 70 may further include a strip-shaped protrusion (as shown in FIG. 7 ) arranged along the axial direction of the pipe body 20 . The strip-shaped protrusion can be matched with the slot of the external device to fix the mixing tube 10 with the external device.
  • the mixing tube 10 may further include a top cover (not shown in the figure), and the top cover may be covered on the open end of the tube body 20 (ie, the end of the inner side wall 210 away from the inner bottom wall 220 ).
  • the cap and mixing tube 10 can be mated in various ways. Including but not limited to threaded 80 connection, snap connection, etc.
  • the end of the outer wall of the pipe body 20 away from the inner bottom wall 220 is provided with a thread 80
  • the inner wall 200 of the top cover is provided with a thread 80 groove
  • the top cover is connected to the pipe body 20 through the thread 80 and the thread 80 groove.
  • the possible beneficial effects of the embodiments of the present application include, but are not limited to: (1) By arranging the first stirring member and the second stirring member with different ratios of width to thickness or different widths on the inner wall, and The substances in the area are impacted and stirred to improve the mixing rate and mixing effect; (2) The two first stirring members and the two second stirring members are symmetrically arranged relative to the central axis of the pipe body, so that each second stirring member is impacted. The vortex and turbulence generated during cell resuspension do not affect each other; (3) Set the number of the first stirring member and the second stirring member to two, when the sample moves, it will be affected by the two first stirring members and the second stirring member.
  • the two stirring members impact and stir respectively, thereby improving the mixing effect; (4) by setting the angle of the included angle formed by the connecting line between the two first stirring members and the connecting line between the two second stirring members to be 90 degrees, so that the eddy current and turbulent flow generated when each second stirring member impacts the cell resuspension do not affect each other; (5) By setting the anti-rotation part on the outer wall of the tube body, when the mixing tube is matched with the external device, The anti-rotation part can also be matched with an external device to fix the mixing tube to the external device to ensure that the mixing tube will not be separated when it rotates under the drive of the external device; (6) Set the bottom of the tube body to be flat or upper The concave surface reduces the accumulation of solid substances at the bottom and makes the mixing between substances more uniform. It should be noted that different embodiments may have different beneficial effects, and in different embodiments, the possible beneficial effects may be any one or a combination of the above, or any other possible beneficial effects.
  • the present application uses specific words to describe the embodiments of the present application.
  • Such as “one embodiment,” “an embodiment,” and/or “some embodiments” means a certain feature, structure, or characteristic associated with at least one embodiment of the present application.
  • two or more references to “an embodiment” or “an embodiment” in various places in this application are not necessarily referring to the same embodiment.
  • certain features, structures or characteristics of the one or more embodiments of the present application may be combined as appropriate.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Mixers Of The Rotary Stirring Type (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

一种混匀管（10），包括：管体（20），管体（20）用于容纳样本；设置在管体（20）的内壁（200）上的第一搅拌件（30）和第二搅拌件（40），第一搅拌件（30）的宽度与厚度之比大于第二搅拌件（30）的宽度与厚度之比。

Description

混匀管
相关申请的交叉引用
本申请要求2021年4月6日提交的申请号为202110369584.X的中国专利申请的优先权，其全部内容通过引入并入本文。
技术领域
本申请涉及物质混合领域，特别涉及一种混匀管。
背景技术
在生物工程的实际操作中，通常需要将两种或以上的物质进行混合以得到新的混合物或者使两种或以上的物质充分发生反应。物质之间混合效果会影响生物工程产物的质量。
因此，亟需提供一种混匀管，能够加快物质之间的混合速率，并有效提高物质之间的混合效果。
发明内容
本说明书一些实施例提供了一种混匀管，所述混匀管包括：管体，所述管体用于容纳样本；设置在所述管体的内壁上的第一搅拌件和第二搅拌件，所述第一搅拌件的宽度与厚度之比大于所述第二搅拌件的宽度与厚度之比。
在一些实施例中，所述第一搅拌件的宽度与厚度之比大于3。
在一些实施例中，所述第一搅拌件的宽度在3.5mm～5mm范围内，所述第一搅拌件的厚度在1mm～1.2mm范围内。
在一些实施例中，所述第二搅拌件的宽度与厚度之比小于1.5。
在一些实施例中，所述第二搅拌件的宽度在1.6mm～1.9mm范围内，所述第二搅拌件的厚度在1.2mm～1.4mm范围内。
在一些实施例中，所述第一搅拌件的高度在5mm～100mm范围内。
在一些实施例中，所述第二搅拌件的高度在10mm～100mm范围内。
在一些实施例中，所述第一搅拌件在宽度方向上与所述管体的内壁设有间隙。
在一些实施例中，所述间隙的长度不大于10mm。
在一些实施例中，所述管体的内壁包括内侧壁和封闭连接在所述内侧壁一端的内底壁；所述第一搅拌件设置在所述内底壁上，所述第二搅拌件设置在所述内侧壁上。
在一些实施例中，所述内底壁的底部为平面或上凸面，所述上凸面朝向所述管 体的内部凸出。
在一些实施例中，所述内底壁的底部设置有朝向所述管体的内部凸出的凸起部。
在一些实施例中，所述第一搅拌件和所述第二搅拌件的数量均为两个。
在一些实施例中，两个所述第一搅拌件与两个所述第二搅拌件均相对所述管体的中心轴线对称设置。
在一些实施例中，两个所述第一搅拌件之间的连线与两个所述第二搅拌件之间的连线所形成的夹角的角度为90度。
在一些实施例中，所述第一搅拌件与所述第二搅拌件沿所述管体的内壁周向间隔设置。
在一些实施例中，所述第一搅拌件包括第一端和第二端，所述第一端与所述管体的内壁连接，所述第二端向所述管体的中心延伸。
附图说明
本申请将以示例性实施例的方式进一步说明，这些示例性实施例将通过附图进行详细描述。这些实施例并非限制性的，在这些实施例中，相同的编号表示相同的结构，其中：
图1是根据本申请一些实施例所示的管体的部分结构示意图；
图2是根据本申请一些实施例所示的混匀管的结构示意图；
图3是图2的俯视图；
图4是根据本申请另一些实施例所示的管体的部分结构示意图；
图5是根据本申请又一些实施例所示的管体的部分结构示意图；
图6是根据本申请另一些实施例所示的混匀管的结构透视图；
图7是根据本申请另一些实施例所示的混匀管的结构示意图；
图8是根据本申请再一些实施例所示的管体的部分结构示意图；
图9是根据本申请一些实施例所示的混匀管的剖面示意图；
图10是根据本申请另一些实施例所示的混匀管的剖面示意图。
附图标记：混匀管10；管体20；内壁200；内侧壁210；内底壁220；凸起部221；第一搅拌件30；第二搅拌件40；间隙50；第一防转部60；第二防转部70；螺纹80。
具体实施方式
为了更清楚地说明本申请实施例的技术方案，下面将对实施例描述中所需要使 用的附图作简单的介绍。显而易见地，下面描述中的附图仅仅是本申请的一些示例或实施例，对于本领域的普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图将本申请应用于其它类似情景。除非从语言环境中显而易见或另做说明，图中相同标号代表相同结构或操作。
如本申请和权利要求书中所示，除非上下文明确提示例外情形，“一”、“一个”、“一种”和/或“该”等词并非特指单数，也可包括复数。一般说来，术语“包括”与“包含”仅提示包括已明确标识的步骤和元素，而这些步骤和元素不构成一个排它性的罗列，方法或者设备也可能包含其他的步骤或元素。术语“基于”是“至少部分地基于”。术语“一个实施例”表示“至少一个实施例”；术语“另一实施例”表示“至少一个另外的实施例”；术语“在…范围内”表示数值范围时，包含两个端值，如在1.5mm-15mm范围内，表示的范围除1.5mm-15mm之间的数值外，还包含1.5mm和15mm。其他术语的相关定义将在下文描述中给出。
在生物工程的实际操作中，通常需要将两种或以上的物质进行混合，混合的目的包括但不限于得到新的混合物或者使两种或以上的物质能够充分发生反应。在一些实施例中，混合两种或以上的物质的步骤可以包括将两种或以上的物质(也即样本)放入混匀管中，然后利用外部装置(例如，混匀装置)旋转或操作人员手动旋转混匀管，使得混匀管中的两种或以上的物质进行离心运动，进而实现混合的目的。
然而，由于每种物质的比重不同，因此当两种或以上的物质容置在混匀管中时会分别处于混匀管中的不同区域(例如，一种固态物质比重较大，主要堆积在混匀管的底部，一种液态物质比重较小，主要处混匀管的除底部外的其他区域)。
在一些实施例中，可以在混匀管中设置辅助搅拌结构来帮助提高混合效果。在一些实施例中，辅助搅拌结构可以包括设置在混匀管内壁上的搅拌筋。利用搅拌筋可以使混匀管内的液态物质在混匀过程中产生旋涡，旋涡将液态物质引入到固态物质所在的区域从而实现混合。在一些实施例中，辅助搅拌结构可以包括设置在混匀管内壁上的搅拌片。利用搅拌片可以在混匀过程中对待混匀物质进行冲击，对堆积在一起的物质进行打散，从而实现充分混合。在一些实施例中，辅助搅拌结构可以同时包括设置在混匀管内壁上的搅拌片和搅拌筋。利用搅拌片将堆积在一起的物质进行打散、利用搅拌筋产生旋涡，从而实现更加充分的混匀，提高混合效果。在一些实施例中，搅拌片与搅拌筋相比，搅拌片的宽度与厚度比值大于搅拌筋的宽度与厚度的比值，也可以说搅拌片比搅拌筋更薄。在一些实施例中，搅拌片与搅拌筋相比，搅拌片的宽度大于搅拌筋的宽度，也 可以说搅拌片比搅拌筋更宽。
通过本申请提供的混匀管进行搅拌混合时，搅拌筋和搅拌片可以分别对处于管体中不同区域的物质进行冲击、搅拌，让物质运动更剧烈，使得物质之间的混合更充分，提高混合效果。下面将结合附图来对混匀管进行示例性描述。
参见图1所示，在一些实施例中，混匀管10可以包括用于容纳样本(图中未示出)的管体20。管体20的内壁200上设置有第一搅拌件30和第二搅拌件40。在一些实施例中，第一搅拌件30的宽度和厚度之比大于第二搅拌件40的宽度和厚度之比，可以对样本进行更充分地混合、搅拌，提高混合效果。此外，在一些实施例中，可以使得第一搅拌件30的宽度大于第二搅拌件40的宽度，同样可以实现上述效果。在一些实施例中，搅拌件(例如，第一搅拌件30、第二搅拌件40)的宽度是指搅拌件在从管体20的内壁向管体20的内部延伸的方向上的尺寸。例如，图3中第一搅拌件30的宽度X1以及第二搅拌件40的宽度X2。搅拌件的厚度是指搅拌件从管体20的内壁向管体20的内部延伸出去的两个侧面之间距离。例如，图3中第一搅拌件30的厚度Y1，第二搅拌件40的厚度Y2。在一些实施例中，第一搅拌件30的宽度X1和厚度Y1之比大于第二搅拌件40的宽度X2和厚度Y2之比，所以第一搅拌件30比第二搅拌件40更薄，因此，第一搅拌件30也可以叫搅拌片，第二搅拌件40也可以叫搅拌筋。
本申请中的混匀管10可以作为样本混合的容器。所谓样本混合可以理解为将样本中包含的两种或以上的物质进行混合。在一些实施例中，两种或以上的物质进行混合可以包括固态物质与液态物质混合以及液态物质与液态物质混合等。例如，将大肠杆菌菌泥(即，对大肠杆菌培养液进行离心分离后得到的固态物质)与细胞重悬液进行混合。为了描述方便，除特别说明之外，本申请均以大肠杆菌菌泥与细胞重悬液混合作为示例进行说明。
需要说明的是，本申请一个或多个实施例仅作为示例描述混匀管10的其中一种用途。可以理解的是，混匀管10的适用场景并不仅限于此。例如，混匀管10可以作为存储容器，存储样本。又例如，混匀管10可以作为离心分离的容器，与离心装置进行配合将存储在混匀管10中的样本中的比重不同的物质进行离心分离。此外，当混匀管10作为离心分离的容器时，可以称作离心管。还例如，混匀管10可以作为反应容器，存储在混匀管10中的若干样本可以发生反应。还例如，混匀管10可以同时作为离心分离和样本混合的容器(例如，在混匀管10中对大肠杆菌培养液进行离心分离得到大肠杆菌菌泥，然后将大肠杆菌菌泥与细胞重悬液混合)。
在一些实施例中，样本可以包括待混合的两种或两种以上物质，例如，样本可以包括大肠杆菌菌泥和细胞重悬液。
第一搅拌件30和第二搅拌件40可以配置为当管体20内的样本在惯性以及离心力的作用下运动时，对样本进行冲击、搅拌。冲击和搅拌可以使样本中的物质发生更剧烈的运动，加快混合速率，并提高物质之间的混合效果。
参见图3所示，在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比大于3。在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比的数值范围为3～20。在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比的数值范围为3～15。在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比的数值范围为3～10。在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比的数值范围为3～4。
在一些实施例中，第一搅拌件30的宽度X1可以在1.5mm～15mm范围内，厚度Y1可以在0.5mm～3mm范围内。在一些实施例中，第一搅拌件30的宽度X1可以在2.5mm～10mm范围内，厚度Y1可以在0.75mm～2mm范围内。在一些实施例中，第一搅拌件30的宽度X1可以在3.5mm～5mm范围内，厚度Y1可以在1mm～1.5mm范围内。优选地，在一些实施例中，第一搅拌件30的宽度X1可以为3.5mm，第一搅拌件30的厚度Y1可以在1mm～1.2mm范围内。
在一些实施例中，第二搅拌件40的宽度X2与厚度Y2之比的小于1.5。在一些实施例中，第二搅拌件40的宽度X2与厚度Y2之比的数值范围为0.1～1.5。在一些实施例中，第二搅拌件40的宽度X2与厚度Y2之比的数值范围为0.5～1.5。在一些实施例中，第二搅拌件40的宽度X2与厚度Y2之比的数值范围为0.75～1.5。在一些实施例中，第二搅拌件40的宽度X2与厚度Y2之比的数值范围为1～1.5。
在一些实施例中，第二搅拌件40的宽度X2可以在1mm～3mm范围内，第二搅拌件40的厚度Y2可以在0.6mm～2mm范围内。在一些实施例中，第二搅拌件40的宽度X2可以在1.2mm～2.5mm范围内，第二搅拌件40的厚度Y2可以在0.8mm～1.8mm范围内。在一些实施例中，第二搅拌件40的宽度X2可以在1.4mm～2mm范围内，第二搅拌件40的厚度Y2可以在1mm～1.6mm范围内。在一些实施例中，第二搅拌件40的宽度X2可以在1.6mm～1.9mm范围内，第二搅拌件40的厚度Y2可以在1.2mm～1.4mm范围内。在一些优选实施例中，第二搅拌件40的宽度X2可以为1.8mm，第二搅拌件40的厚度Y2可以在1.2mm～1.4mm范围内。
在一些实施例中，搅拌筋的截面形状可以为多种形式，包括但不限于类三角形 (即三角形的一边为弧线)、类梯形(即梯形的一边为弧线)或类矩形(即矩形的一边为弧线)。
在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比与第二搅拌件40的宽度X2与厚度Y2之比的差值在0.5～5范围内。在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比与第二搅拌件40的宽度X2与厚度Y2之比的差值在1～4范围内。在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比与第二搅拌件40的宽度X2与厚度Y2之比的差值在1.5～3范围内。在一些优选实施例中，第一搅拌件30的宽度X1与厚度Y1之比与第二搅拌件40的宽度X2与厚度Y2之比的差值在1.5～2范围内。
在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比与第二搅拌件40的宽度X2与厚度Y2之比的比值在1～5范围内。在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比与第二搅拌件40的宽度X2与厚度Y2之比的比值在1.5～4范围内。优选地，在一些实施例中，第一搅拌件30的宽度X1与厚度Y1之比与第二搅拌件40的宽度X2与厚度Y2之比的比值在2～3范围内。
在一些实施例中，第一搅拌件30的宽度X1可以大于第二搅拌件40的宽度X2，以提高混合效果。
在一些实施例中，第一搅拌件30的宽度X1可以在1.5mm～15mm范围内。在一些实施例中，第一搅拌件30的宽度X1可以在2.5mm～10mm范围内。在一些实施例中，第一搅拌件30的宽度X1可以在3.5mm～5mm范围内。优选地，在一些实施例中，第一搅拌件30的宽度X1可以为3.5mm。
在一些实施例中，第二搅拌件40的宽度X2可以在1mm～3mm范围内。在一些实施例中，第二搅拌件40的宽度X2可以在1.2mm～2.5mm范围内。在一些实施例中，第二搅拌件40的宽度X2可以在1.4mm～2mm范围内。在一些实施例中，第二搅拌件40的宽度X2在1.6mm～1.8mm范围内。优选地，在一些实施例中，第二搅拌件40的宽度X2可以为1.8mm。
在一些实施例中，第一搅拌件30的宽度X1与第二搅拌件40的宽度X2的差值可以在0.5mm～14mm范围内。在一些实施例中，第一搅拌件30的宽度X1与第二搅拌件40的宽度X2的差值可以在1mm～10mm范围内。在一些实施例中，第一搅拌件30的宽度X1与第二搅拌件40的宽度X2的差值可以在1.25mm～5mm范围内。在一些实施例中，第一搅拌件30的宽度X1与第二搅拌件40的宽度X2的差值可以在1.5mm～2mm范围内。优选地，在一些实施例中，第一搅拌件30的宽度X1与第二搅拌件40的宽度 X2的差值可以为1.7mm。
在一些实施例中，第一搅拌件30的宽度X1与第二搅拌件40的宽度X2之比在1～5范围内。在一些实施例中，第一搅拌件30的宽度X1与第二搅拌件40的宽度X2之比在1.25～3范围内。在一些实施例中，第一搅拌件30的宽度X1与第二搅拌件40的宽度X2之比在1.5～2.5范围内。优选地，在一些实施例中，第一搅拌件30的宽度X1与第二搅拌件40的宽度X2之比在1.75～2范围内。
在一些实施例，第一搅拌件30和第二搅拌件40的高度与待混合的样本量和管体20的总长S1(如图10所示)有关。在一些实施例中，搅拌件的高度可以是指搅拌件靠近管体20的底部向远离管体20的顶部延伸的方向上尺寸。例如，图9中第一搅拌件30的高度Z1，第二搅拌件40的高度Z2。
在一些实施例中，第一搅拌件30的高度Z1可以在5mm～100mm范围内。在一些实施例中，第一搅拌件30的高度Z1可以在10mm～75mm范围内。在一些实施例中，第一搅拌件30的高度Z1可以在15mm～50mm范围内。优选地，在一些实施例中，第一搅拌件30的高度Z1可以为15mm。在一些实施例中，第二搅拌件40的高度Z2可以在10mm～100mm范围内。在一些实施例中，第二搅拌件40的高度Z2可以在30mm～90mm范围内。在一些实施例中，第二搅拌件40的高度Z2可以在50mm～80mm范围内。在一些实施例中，第二搅拌件40的高度Z2可以在60mm～70mm范围内。优选地，在一些实施例中，第二搅拌件40的高度Z2可以为60mm。
结合图1、图3和图6所示，在一些实施例中，管体20的内壁200可以包括内侧壁210和封闭连接在内侧壁210一端的内底壁220；第一搅拌件30可以设置在内底壁220上，第二搅拌件40可以设置在内侧壁210上。第一搅拌件30和第二搅拌件40可以分别对处于混匀管10中的不同区域的物质进行冲击、搅拌，使样本发生更剧烈的运动。仍然以样本包括大肠杆菌菌泥和细胞重悬液为例，大肠杆菌菌泥由于比重大，因此主要堆积在混匀管10的底部区域，即内底壁220处；而细胞重悬液比重小，则会主要位于混匀管10除底部之外的区域，例如，内侧壁210对应的区域。当混匀管10转动时，大肠杆菌菌泥会在离心力以及惯性的作用下发生运动，第一搅拌件30会冲击、搅拌大肠杆菌菌泥，使其发生更剧烈的运动。类似的，细胞重悬液也会在离心力和惯性的作用下发生运动，第二搅拌件40会冲击、搅拌细胞重悬液，使得细胞重悬液震荡产生涡流和湍流，进而使得细胞重悬液冲击大肠杆菌菌泥，实现充分混合。
在一些实施例中，第一搅拌件30与第二搅拌件40可以沿管体20的内壁200周 向设置。在一些实施例中，第一搅拌件30与第二搅拌件40可以间隔设置。在本实施例中，将第一搅拌件30与第二搅拌件40沿管体20的内壁200周向间隔设置可以使得有效提高样本中的两种或以上物质的混合效果。
在一些实施例中，混匀管10可以与外部装置(例如，混匀装置)配合使用，提高样本的混合效果。在一些实施例中，当混匀装置的旋转方式为角转子旋转(即，将混匀管10倾斜一定角度再进行旋转)时，大肠杆菌菌泥主要堆积在内壁200的一侧。如果第一搅拌件30与内壁200之间没有间隙50则会形成死角，大肠杆菌菌泥会堆积在死角处，无法充分与细胞重悬液混合。
在一些实施例中，第一搅拌件30可以在宽度方向上与管体20内壁200设有间隙50。结合图1和图4所示，由于存在间隙50，所以第一搅拌件30与内壁200之间不会存在死角，能够有效避免大肠杆菌菌泥的堆积，提高混合效果。
在一些实施例中，第一搅拌件30在宽度方向上与管体20的内壁200之间的间隙50的长度H可以不大于10mm。在一些实施例中，第一搅拌件30宽度方向上与管体20的内壁200之间的间隙50的长度H可以在1mm～6mm范围内。在一些实施例中，第一搅拌件30宽度方向上与管体20的内壁200之间的间隙50的长度H可以在1.5mm～3mm范围内。在一些优选实施例中，第一搅拌件30宽度方向上与管体20的内壁200之间的间隙50的长度H可以为2mm。
在一些实施例中，第一搅拌件30可以包括第一端和第二端，第一端可以与管体20的内壁200连接，第二端沿与管体20的中心轴线O(如图9和图10所示)平行的方向延伸。
如图9所示，在一些实施例中，第一搅拌件30的第二端可以向管体20的中心延伸，也即，第一搅拌件30的延伸方向与管体20的中心轴线O方向呈一定夹角，以进一步提高混合效果。
在一些实施例中，第一搅拌件30的第一端可以与管体20的内底壁220连接，例如，在图1、图9所示的实施例中，第一搅拌件30的第一端连接在内底壁220。
参见图10所示，在一些实施例中，第一搅拌件30的第一端可以与管体20的内侧壁210靠近内底壁220的一端连接。在一些实施例中，当第一搅拌件30的第一端可以与管体20的内侧壁210连接时，第一搅拌件30的第一端与内底壁220的底部之间的距离S2可以为管体20总长度S1的1/7～1/3。在一些实施例中，第一搅拌件30的第一端与内底壁220的底部之间的距离S2可以为管体20总长度S1的1/6～1/3。优选地， 在一些实施例中，第一搅拌件30的第一端与内底壁220的底部之间的距离S2可以为管体20总长度S1的1/5～1/3。
此外，为了减少固态物质堆积在第一搅拌件30与管体20的内底壁220的连接处，在一些实施例中，还可以将第一搅拌件30配置为在其宽度方向上与管体20的内底壁220平滑地连接，即在宽度方向上的连接处设有圆角。
在一些实施例中，第一搅拌件30和第二搅拌件40可以对样本进行冲击，从而引起样本更剧烈的运动，提高物质之间的混合效果。在前述一个或多个实施例中介绍了第一搅拌件30和第二搅拌件40的设置位置以及具体结构对混合效果的影响。除此之外，在一些实施例中，第一搅拌件30和第二搅拌件40的数量也同样影响着物质之间的混合效果。
参见图6所示，在一些实施例中，第一搅拌件30和第二搅拌件40的数量可以均为两个。在本实施中，由于第一搅拌件30和第二搅拌件40的数量增加，使得液体(例如，细胞重悬液)在惯性以及离心力的作用下被两个第二搅拌件40所冲击、搅拌，进而产生更加剧烈的涡流和湍流。同时，大肠杆菌菌泥也会被两个第一搅拌件30所冲击、搅拌，产生的运动也更加剧烈，所以，大肠杆菌菌泥与细胞重悬液的混合会更加充分，混合速率也会更快。
在一些实施例中，第一搅拌件30和第二搅拌件40的数量不仅限于都是两个，还可以都是一个、三个、四个或者更多。例如，在图9所示的实施例中，第一搅拌件30和第二搅拌件40的数量都是一个。
在一些实施例中，第一搅拌件30和第二搅拌件40的数量可以是相同的。例如，第一搅拌件30和第二搅拌件40的数量可以均为两个，如图3和图6所示。又例如，在图9所示的实施例中，第一搅拌件30和第二搅拌件40的数量可以均为一个。
在一些实施例中，第一搅拌件30和第二搅拌件40的数量可以是不相同的。例如，第一搅拌件30的数量为一个，第二搅拌件40的数量为两个。又例如，第一搅拌件30的数量为两个，第二搅拌件40的数量为四个。
在一些实施例中，第一搅拌件30和第二搅拌件40的设置方式可以与第一搅拌件30和第二搅拌件40数量相关联。结合图3、图6和图9所示，在一些实施例中，两个第一搅拌件30与两个第二搅拌件40可以均相对管体20的中心轴线O对称设置。在本实施例中，由于对称设置的关系，两个第二搅拌件40可以同时对细胞重悬液进行冲击搅拌，同时两个第一搅拌件30也可以对大肠杆菌菌泥进行冲击搅拌，使得细胞重悬 液与大肠杆菌菌泥能够更均匀地进行混合，提高样本的混合效果。同时，还可以使得每个第二搅拌件40冲击细胞重悬液时产生的涡流和湍流互不影响，进一步提高混合效果。
除此之外，在一些实施例中，当第一搅拌件30的数量三个时，三个第一搅拌件30可以以相同的间隔环绕设置在内底壁220，即相邻两个第一搅拌件30与管体20的中心轴线O的连线形成的夹角为120度。在一些实施例中，当第一搅拌件30的数量为四个时，四个第一搅拌件同样可以以相同的间隔环绕设置在内底壁220，即相邻两个第一搅拌件30与管体20的中心轴线O的连线形成的夹角为90度。类似的，对于第二搅拌件40的设计也可以参考第一搅拌件30的实施例的描述，例如，当第二搅拌件40的数量三个时，三个第二搅拌件40可以以相同的间隔环绕设置在内侧壁210，相邻两个第二搅拌件40与管体20的中心轴线O的连线形成的夹角为120度。又例如，当第二搅拌件40的数量四个时，四个第二搅拌件40可以以相同的间隔环绕设置在内侧壁210，相邻两个第二搅拌件40与管体20的中心轴线O的连线形成的夹角为90度。
需要说明的是，本实施例中仅作为示例说明第一搅拌件30和第二搅拌件40的设置方式，在掌握混匀管10的原理之后，可以对第一搅拌件30和第二搅拌件40的设置方式进行改进。例如，两个第一搅拌件30可以相对管体20的中心轴线O对称设置，而第二搅拌件40并不相对管体20中心轴线O对称。又例如，第一搅拌件30的数量为四个，第二搅拌件40的数量为三个，三个第二搅拌件40可以以相同的间隔环绕设置在内侧壁210，而四个第一搅拌件30可以以不同的间隔环绕设置在内底壁220。诸如此类的变形均在本申请的保护范围内。
图3中示例性地示出了两个第一搅拌件30之间的连线和两个第二搅拌件40之间的连线形成的夹角β的角度为90度的实施例。结合图3和图6所示，在一些实施例中，样本的混合效果还与两个第一搅拌件30之间的连线与两个第二搅拌件40之间的连线所形成的夹角的角度有关。在一些实施例中，两个第一搅拌件30之间的连线与两个第二搅拌件40之间的连线所形成的夹角β的角度范围包括30度～90度。在一些实施例中，两个第一搅拌件30之间的连线与两个第二搅拌件40之间的连线所形成的夹角β的角度范围包括45度～90度。在一些实施例中，两个第一搅拌件30之间的连线与两个第二搅拌件40之间的连线所形成的夹角β的角度范围包括60度～90度。在一些优选实施例中，两个第一搅拌件30之间的连线与两个第二搅拌件40之间的连线所形成的夹角β的角度为90度。
在一些实施例中，当第一搅拌件30和第二搅拌件40的数量为三个时，三个第 一搅拌件30可以以相同的间隔环绕设置在内底壁220，三个第二搅拌件40可以以相同的间隔环绕设置在内侧壁210，第一搅拌件30与管体20中心轴线O的连线与第二搅拌件40与管体20中心轴线O的连线的夹角可以为60度。在一些实施例中，当第一搅拌件30和第二搅拌件的数量均为四个时，四个第一搅拌件30可以以相同的间隔环绕设置在内底壁220，四个第二搅拌件40可以以相同的间隔环绕设置在内侧壁210，第一搅拌件30与管体20中心轴线O的连线与第二搅拌件40与管体20中心轴线O的连线的夹角可以为45度。
在一些实施例中，若第一搅拌件30的数量与第二搅拌件40的数量不同，例如，当第一搅拌件30的数量为四个，第二搅拌件40的数量为两个时，两个第一搅拌件30可以相对管体20的中心轴线O对称设置在内底壁220，四个第二搅拌件40可以以相同的间隔环绕设置在内侧壁210，第一搅拌件30与管体20中心轴线O的连线与第二搅拌件40与管体20中心轴线O的连线的夹角可以为30度。又例如，当第一搅拌件30的数量为一个，第二搅拌件40的数量为两个时，两个第二搅拌件40可以相对管体20的中心轴线O对称设置在内侧壁210，第一搅拌件30与管体20的中心轴线O的连线与两个第二搅拌件40之间的连线形成的夹角可以为90度。
在一些实施例中，制作第一搅拌件30和第二搅拌件40的材料可以与制作管体20的材料相同，包括聚乙烯、聚碳酸酯、聚丙烯等。在一些实施例中，第一搅拌件30、第二搅拌件40与管体20可以一体成型，也可以分体成型然后进行组装。
在一些实施例中，混匀管10可以仅包括第一搅拌件30，第一搅拌件30可以沿管体20的内壁200周向设置，通过第一搅拌件30可以实现冲击、搅拌样本，提高混合效果的目的。在另一些实施例中，混匀管10可以仅包括第一搅拌件30，第一搅拌件30可以沿管体20的内壁200周向设置，并且第一搅拌件30在宽度方向上与管体20的内壁200设有间隙50。当混匀管10中只有第一搅拌件30时，第一搅拌件30的数量可以一个、两个、三个或更多，一个或多个第一搅拌件30的设置方式可以参见本申请其他实施例的描述，此处不再赘述。当混匀管1中只包括第一搅拌件30时，第一搅拌件30的宽度、厚度、宽度与厚度的比值、高度以及第一搅拌件30的设置位置等相关设计都可以参照本说明书中其他实施例部分对第一搅拌件30的描述，此处不再赘述。
在一些实施例中，物质之间的混合主要依靠混匀管10做往复旋转运动时产生的离心力以及样本的惯性使得物质发生运动，并进一步被第一搅拌件30和第二搅拌件40冲击、搅拌实现混合。可以理解的是，越靠近混匀管10中心轴线O，在混匀管10转动 时受到的离心力和惯性力就越小。在一些实施例中，管体20的内底壁220可以沿背离管体20的方向向外凸出形成锥体，如果锥体的顶角在其母线所在的平面内的角度较小时，则大肠杆菌菌泥可能会较容易地堆积在内底壁220的底部，无法与细胞重悬液充分混合，降低了混合效果。
为了避免大肠杆菌菌泥的堆积，提高混合效果，可以对内底壁220的底部进行设计。在一些实施例中，可以将内底壁220设置为背离管体20的方向向外凸出形成锥体，并使得锥体的顶角在其母线所在的平面内的角度大于90度。在一些实施例中，可以将内底壁220的底部设置为平面，即管体20为平底，如图9和图10所示。当管体20为平底时，可以有效减少大肠杆菌菌泥在底部的堆积。在一些实施例中，可以将内底壁220的底部设置为上凸面，所谓上凸面可以是指内底壁220的底部朝向管体20的内部凸出。由于内底壁220的底部朝上(即管体20的内部)凸出，即使大肠杆菌菌泥受到的离心力和惯性力较小，也无法在上凸面堆积。
在一些实施例中，除了对内底壁220的底部进行结构设计之外，还可以通过在内底壁220的底部设置朝向管体20的内部凸出的凸起部221来防止大肠杆菌菌泥的堆积，提高混合效果。在一些实施例中，凸起部221的形状可以为锥体、柱体、半球体、半椭圆球体等或其组合。例如，凸起部221可以为圆锥体。又例如，凸起部221可以为半球体。
在一些实施例中，凸起部221的边缘与内底壁220的连接处可以为平滑连接，即连接处为弧面，以避免大肠杆菌菌泥堆积在凸起部221与内底壁220的连接处，提高混合效果。
需要说明的是，凸起部221可以与前述一个或多个实施例中与内底壁220的底部相关的结构进行结合。例如，内底壁220的底部可以为平面，同时在内底壁220的底部还设置有朝向管体20的内部凸出的凸起部221。
结合图4至图6所示，在一些实施例中，管体20的外壁可以设置有防转部，当混匀管10与外部装置配接时，防转部可以用于防止混匀管10与外部装置配接后发生相对运动。这里的外部装置根据混匀管10的用途不同而不同，例如，当需要对混匀管10中的样本进行混合时，外部装置可以是混匀装置。又例如，当需要对混匀管10中的样本进行离心分离时，外部装置可以是离心装置。
在一些实施例中，防转部可以包括设置在管体20的外壁远离内底壁220一端的第一防转部60。在一些实施例中，第一防转部60可以为环绕设置在管体20的外壁的 凸缘(如图4所示)。当混匀管10与外部装置配接时，凸缘也可以与外部装置配接，保证混匀管10在外部装置的驱动下旋转时不会脱离。
结合图7和图8所示，在一些实施例中，防转部可以进一步包括设置在管体20的外侧壁的第二防转部70。在一些实施例中，第二防转部70可以为沿管体20轴线方向设置的凹槽。在一些实施例中，凹槽可以与外部装置的卡扣配接，进而保证混匀管10与外部装置的相对固定。在一些实施例中，第二防转部70还可以包括沿管体20的轴线方向设置的条状凸起(如图7所示)。条状凸起可以与外部装置的卡槽配接，将混匀管10与外部装置固定。
在一些实施例中，混匀管10还可以包括顶盖(图中未示出)，顶盖可以盖设于管体20的开口端(即，内侧壁210的远离内底壁220的一端)。在一些实施例中，顶盖与混匀管10可以通过多种方式进行配接。包括但不限于螺纹80连接、卡扣连接等。例如，在管体20的外壁的远离内底壁220的一端设置有螺纹80，顶盖的内壁200设置有螺纹80槽，通过螺纹80与螺纹80槽的将顶盖与管体20连接。
本申请实施例可能带来的有益效果包括但不限于：(1)通过在内壁上设置宽度与厚度的比值不同或者宽度不同的第一搅拌件和第二搅拌件，同时对位于管体中不同区域的物质进行冲击、搅拌，提高混合速率和混合效果；(2)将两个第一搅拌件与两个第二搅拌件均相对管体的中心轴线对称设置，使得每个第二搅拌件冲击细胞重悬液时产生的涡流和湍流互不影响；(3)将第一搅拌件和第二搅拌件的数量设置为两个，在样本发生运动时，会被两个第一搅拌件和第二搅拌件分别冲击、搅拌，进而提高混合效果；(4)通过将两个第一搅拌件之间的连线与两个第二搅拌件之间的连线所形成的夹角的角度设置为90度，使得每个第二搅拌件冲击细胞重悬液时产生的涡流和湍流互不影响；(5)通过在管体的外壁设置防转部，当混匀管与外部装置配接时，防转部也可以与外部装置配接，将混匀管与外部装置固定，保证混匀管在外部装置的驱动下进行旋转时不会脱离；(6)将管体的底部设置为平底或上凹面，减少堆积在底部的固态物质，使物质之间的混合更均匀。需要说明的是，不同实施例可能产生的有益效果不同，在不同的实施例里，可能产生的有益效果可以是以上任意一种或几种的组合，也可以是其他任何可能获得的有益效果。
上文已对基本概念做了描述，显然，对于本领域技术人员来说，上述详细披露仅仅作为示例，而并不构成对本申请的限定。虽然此处并没有明确说明，本领域技术人员可能会对本申请进行各种修改、改进和修正。该类修改、改进和修正在本申请中被建 议，所以该类修改、改进、修正仍属于本申请示范实施例的精神和范围。
同时，本申请使用了特定词语来描述本申请的实施例。如“一个实施例”、“一实施例”、和/或“一些实施例”意指与本申请至少一个实施例相关的某一特征、结构或特点。因此，应强调并注意的是，本申请中在不同位置两次或多次提及的“一实施例”或“一个实施例”并不一定是指同一实施例。此外，本申请的一个或多个实施例中的某些特征、结构或特点可以进行适当的组合。
此外，除非权利要求中明确说明，本申请所述处理元素和序列的顺序、数字字母的使用、或其他名称的使用，并非用于限定本申请流程和方法的顺序。尽管上述披露中通过各种示例讨论了一些目前认为有用的发明实施例，但应当理解的是，该类细节仅起到说明的目的，附加的权利要求并不仅限于披露的实施例，相反，权利要求旨在覆盖所有符合本申请实施例实质和范围的修正和等价组合。例如，虽然以上所描述的系统组件可以通过硬件设备实现，但是也可以只通过软件的解决方案得以实现，如在现有的服务器或移动设备上安装所描述的系统。
同理，应当注意的是，为了简化本申请披露的表述，从而帮助对一个或多个发明实施例的理解，前文对本申请实施例的描述中，有时会将多种特征归并至一个实施例、附图或对其的描述中。但是，这种披露方法并不意味着本申请对象所需要的特征比权利要求中提及的特征多。实际上，实施例的特征要少于上述披露的单个实施例的全部特征。
最后，应当理解的是，本申请中所述实施例仅用以说明本申请实施例的原则。其他的变形也可能属于本申请的范围。因此，作为示例而非限制，本申请实施例的替代配置可视为与本申请的教导一致。相应地，本申请的实施例不仅限于本申请明确介绍和描述的实施例。

Claims (17)

  1. 一种混匀管，所述混匀管包括：
    管体，所述管体用于容纳样本；
    设置在所述管体的内壁上的第一搅拌件和第二搅拌件，所述第一搅拌件的宽度与厚度之比大于所述第二搅拌件的宽度与厚度之比。
  2. 根据权利要求1所述的混匀管，所述第一搅拌件的宽度与厚度之比大于3。
  3. 根据权利要求1所述的混匀管，所述第一搅拌件的宽度在3.5mm～5mm范围内，所述第一搅拌件的厚度在1mm～1.2mm范围内。
  4. 根据权利要求1所述的混匀管，所述第二搅拌件的宽度与厚度之比小于1.5。
  5. 根据权利要求1所述的混匀管，所述第二搅拌件的宽度在1.6mm～1.9mm范围内，所述第二搅拌件的厚度在1.2mm～1.4mm范围内。
  6. 根据权利要求1所述的混匀管，所述第一搅拌件的高度在5mm～100mm范围内。
  7. 根据权利要求1所述的混匀管，所述第二搅拌件的高度在10mm～100mm范围内。
  8. 根据权利要求1所述的混匀管，所述第一搅拌件在宽度方向上与所述管体的内壁设有间隙。
  9. 根据权利要求8所述的混匀管，所述间隙的长度不大于10mm。
  10. 根据权利要求1所述的混匀管，所述管体的内壁包括内侧壁和封闭连接在所述内侧壁一端的内底壁；
    所述第一搅拌件设置在所述内底壁上，所述第二搅拌件设置在所述内侧壁上。
  11. 根据权利要求10所述的混匀管，所述内底壁的底部为平面或上凸面，所述上 凸面朝向所述管体的内部凸出。
  12. 根据权利要求10所述的混匀管，所述内底壁的底部设置有朝向所述管体的内部凸出的凸起部。
  13. 根据权利要求1所述的混匀管，所述第一搅拌件和所述第二搅拌件的数量均为两个。
  14. 根据权利要求13所述的混匀管，两个所述第一搅拌件与两个所述第二搅拌件均相对所述管体的中心轴线对称设置。
  15. 根据权利要求14所述的混匀管，两个所述第一搅拌件之间的连线与两个所述第二搅拌件之间的连线所形成的夹角的角度为90度。
  16. 根据权利要求1所述的混匀管，所述第一搅拌件与所述第二搅拌件沿所述管体的内壁周向间隔设置。
  17. 根据权利要求1所述的混匀管，所述第一搅拌件包括第一端和第二端，所述第一端与所述管体的内壁连接，所述第二端向所述管体的中心延伸。
PCT/CN2022/085299 2021-04-06 2022-04-06 混匀管 WO2022213977A1 (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202280025955.5A CN117098979A (zh) 2021-04-06 2022-04-06 混匀管
US18/465,964 US20230415109A1 (en) 2021-04-06 2023-09-12 Blending tube

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202110369584.X 2021-04-06
CN202110369584 2021-04-06

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US18/465,964 Continuation US20230415109A1 (en) 2021-04-06 2023-09-12 Blending tube

Publications (1)

Publication Number Publication Date
WO2022213977A1 true WO2022213977A1 (zh) 2022-10-13

Family

ID=83545988

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2022/085299 WO2022213977A1 (zh) 2021-04-06 2022-04-06 混匀管

Country Status (3)

Country Link
US (1) US20230415109A1 (zh)
CN (1) CN117098979A (zh)
WO (1) WO2022213977A1 (zh)

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11128712A (ja) * 1997-10-27 1999-05-18 Kdk Corp 攪拌装置
CN202725091U (zh) * 2012-08-14 2013-02-13 北京尚精光电技术有限公司 搅拌混匀装置
CN103957863A (zh) * 2011-10-20 2014-07-30 贝克顿·迪金森公司 用于容器组件的混合元件
CN106000204A (zh) * 2016-07-31 2016-10-12 浙江大学 用于漩涡振荡器的混匀管
CN207287269U (zh) * 2017-09-22 2018-05-01 浙江中鼎检测技术有限公司 一种样本检测混合搅拌罐
CN207838860U (zh) * 2017-12-06 2018-09-11 成都九执星科技有限公司 一种用于聚羧酸减水剂配置的搅拌杯
CN208727344U (zh) * 2018-08-17 2019-04-12 云梦嘉邦斯新型材料有限公司 一种生产纸张挺硬剂用搅拌装置
CN209406362U (zh) * 2018-12-18 2019-09-20 上海市动物疫病预防控制中心 用于提取检测物的试管
CN210206645U (zh) * 2019-05-27 2020-03-31 海南高晖农业投资有限公司 一种香皂制作用油碱混合搅拌釜
US20200132573A1 (en) * 2018-10-24 2020-04-30 Hamid Badiei Sample vials, rack mounts and sampling devices using them
CN211755123U (zh) * 2020-03-09 2020-10-27 广西壮族自治区农业科学院 一种离心管

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11128712A (ja) * 1997-10-27 1999-05-18 Kdk Corp 攪拌装置
CN103957863A (zh) * 2011-10-20 2014-07-30 贝克顿·迪金森公司 用于容器组件的混合元件
CN202725091U (zh) * 2012-08-14 2013-02-13 北京尚精光电技术有限公司 搅拌混匀装置
CN106000204A (zh) * 2016-07-31 2016-10-12 浙江大学 用于漩涡振荡器的混匀管
CN207287269U (zh) * 2017-09-22 2018-05-01 浙江中鼎检测技术有限公司 一种样本检测混合搅拌罐
CN207838860U (zh) * 2017-12-06 2018-09-11 成都九执星科技有限公司 一种用于聚羧酸减水剂配置的搅拌杯
CN208727344U (zh) * 2018-08-17 2019-04-12 云梦嘉邦斯新型材料有限公司 一种生产纸张挺硬剂用搅拌装置
US20200132573A1 (en) * 2018-10-24 2020-04-30 Hamid Badiei Sample vials, rack mounts and sampling devices using them
CN209406362U (zh) * 2018-12-18 2019-09-20 上海市动物疫病预防控制中心 用于提取检测物的试管
CN210206645U (zh) * 2019-05-27 2020-03-31 海南高晖农业投资有限公司 一种香皂制作用油碱混合搅拌釜
CN211755123U (zh) * 2020-03-09 2020-10-27 广西壮族自治区农业科学院 一种离心管

Also Published As

Publication number Publication date
US20230415109A1 (en) 2023-12-28
CN117098979A (zh) 2023-11-21

Similar Documents

Publication Publication Date Title
US10632434B2 (en) Apparatus for accommodation and dispensing of miscible materials having discharge opening provided with a displacer apparatus
JP4906009B2 (ja) 実験用容器の内容物を混合するためのマルチステーション装置
JP2014124540A (ja) 撹拌装置
TWI526245B (zh) 攪拌裝置及齒輪組
CN101987285A (zh) 搅拌翼和密闭式搅拌装置
KR20130112491A (ko) 교반기
JP4261188B2 (ja) 実験室用試験容器内の材料を撹拌部材により混合し均質化するための装置
US9956534B2 (en) Method and device for suspending cells
WO2022213977A1 (zh) 混匀管
JP7243121B2 (ja) 卓上攪拌遠心機
WO2013156199A1 (en) Baffle system and magnetic mixing system comprising such baffle system
US20180345237A1 (en) Stirrer set and stirring device
CN217449991U (zh) 混匀管
US7407322B2 (en) Tripod-mounted magnetic mixer apparatus and method
EP2944373B1 (en) Device for suspending cells
CN211159539U (zh) 一种涡旋振荡器
KR200441163Y1 (ko) 마그네틱 교반기용 임펠러
JP2015085218A (ja) 撹拌装置
CN110139706B (zh) 平行搅拌叶片
JP5755474B2 (ja) 攪拌装置
JP6169207B1 (ja) 攪拌用回転体および攪拌装置
JP2014136203A (ja) 攪拌器
CN105817189B (zh) 一种带有底伸式搅拌装置的反应釜
TW200400161A (en) Stirring device for the decomposition of cords in a glass melt
CN111330475A (zh) 一种搅拌棍

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22784043

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 202280025955.5

Country of ref document: CN

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 22784043

Country of ref document: EP

Kind code of ref document: A1