WO2022194312A3 - 石榴种质化学离体保存方法 - Google Patents

石榴种质化学离体保存方法 Download PDF

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Publication number
WO2022194312A3
WO2022194312A3 PCT/CN2022/104011 CN2022104011W WO2022194312A3 WO 2022194312 A3 WO2022194312 A3 WO 2022194312A3 CN 2022104011 W CN2022104011 W CN 2022104011W WO 2022194312 A3 WO2022194312 A3 WO 2022194312A3
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WO
WIPO (PCT)
Prior art keywords
germplasm
chemical
vitro preservation
punica granatum
preservation method
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PCT/CN2022/104011
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English (en)
French (fr)
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WO2022194312A2 (zh
Inventor
钱晶晶
王宁
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安徽科技学院
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Publication date
Application filed by 安徽科技学院 filed Critical 安徽科技学院
Priority to ZA2022/10519A priority Critical patent/ZA202210519B/en
Publication of WO2022194312A2 publication Critical patent/WO2022194312A2/zh
Publication of WO2022194312A3 publication Critical patent/WO2022194312A3/zh

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002—Culture media for tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

提供了一种石榴种质化学离体保存用培养基，该培养基为：WPM+IBA 0.5-0.7mg•L-1+琼脂6g•L-1+蔗糖20-30g•L-1+多效唑5-9mg•L-1。该培养基用于石榴种质化学离体保存，能够改善在离体保存过程中出现的褐化问题，提高了离体保存时间，在保存180d时仍可保证组培苗100％的存活率。
PCT/CN2022/104011 2021-07-26 2022-07-06 石榴种质化学离体保存方法 WO2022194312A2 (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ZA2022/10519A ZA202210519B (en) 2021-07-26 2022-09-22 Method for chemical in-vitro conservation of pomegranate germplasm

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202110842946.2 2021-07-26
CN202110842946.2A CN113557959B (zh) 2021-07-26 2021-07-26 石榴种质化学离体保存方法

Publications (2)

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WO2022194312A2 WO2022194312A2 (zh) 2022-09-22
WO2022194312A3 true WO2022194312A3 (zh) 2022-11-24

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PCT/CN2022/104011 WO2022194312A2 (zh) 2021-07-26 2022-07-06 石榴种质化学离体保存方法

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CN (1) CN113557959B (zh)
WO (1) WO2022194312A2 (zh)
ZA (1) ZA202210519B (zh)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113557959B (zh) * 2021-07-26 2022-05-31 安徽科技学院 石榴种质化学离体保存方法
CN116649220B (zh) * 2023-07-19 2024-04-23 广西壮族自治区林业科学研究院 一种石斛组培苗离体保存的方法
CN116941530B (zh) * 2023-07-28 2024-05-14 广西壮族自治区药用植物园 黄花蒿离体保存方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082147A (zh) * 2014-07-16 2014-10-08 江苏省中国科学院植物研究所 细柱五加的离体快速繁殖方法
CN104770305A (zh) * 2015-05-08 2015-07-15 桂林伯林生物技术有限公司 一种罗汉果种质资源离体保存的方法及保存后恢复生长的方法
CN105265319A (zh) * 2015-11-18 2016-01-27 广西壮族自治区药用植物园 一种地枫皮的离体保存方法
WO2019006470A1 (en) * 2017-06-30 2019-01-03 Booshoot Llc MEDIA FOR RAPID AND RELIABLE TISSUE CULTURE OF PLANTS
CN113557959A (zh) * 2021-07-26 2021-10-29 安徽科技学院 石榴种质化学离体保存方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082147A (zh) * 2014-07-16 2014-10-08 江苏省中国科学院植物研究所 细柱五加的离体快速繁殖方法
CN104770305A (zh) * 2015-05-08 2015-07-15 桂林伯林生物技术有限公司 一种罗汉果种质资源离体保存的方法及保存后恢复生长的方法
CN105265319A (zh) * 2015-11-18 2016-01-27 广西壮族自治区药用植物园 一种地枫皮的离体保存方法
WO2019006470A1 (en) * 2017-06-30 2019-01-03 Booshoot Llc MEDIA FOR RAPID AND RELIABLE TISSUE CULTURE OF PLANTS
CN113557959A (zh) * 2021-07-26 2021-10-29 安徽科技学院 石榴种质化学离体保存方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
刘威等 (LIU, WEI ET AL.): "国内软籽石榴栽培品种及研究进展 (Research Progress of Cultivars of Soft-Seed Pomegranate in Domestic)", 北方农业学报 (JOURNAL OF NORTHERN AGRICULTURE), no. 4,, 20 August 2020 (2020-08-20) *
王纪 (WANG, JI): "青钱柳组织培养及次生代谢物调控的初步研究 (Preliminary Studies on Tissue Culture and Secondary Metabolites of Cyclocarya Paliuru)", 中国优秀硕士学位论文全文数据库农业科技辑 (CHINA MASTER’S THESES FULL-TEXT DATABASE (AGRICULTURAL TECHNOLOGY)), no. 2,, 15 February 2010 (2010-02-15) *

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Publication number Publication date
ZA202210519B (en) 2023-02-22
WO2022194312A2 (zh) 2022-09-22
CN113557959A (zh) 2021-10-29
CN113557959B (zh) 2022-05-31

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