WO2022192083A1 - Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers - Google Patents

Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers Download PDF

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Publication number
WO2022192083A1
WO2022192083A1 PCT/US2022/018909 US2022018909W WO2022192083A1 WO 2022192083 A1 WO2022192083 A1 WO 2022192083A1 US 2022018909 W US2022018909 W US 2022018909W WO 2022192083 A1 WO2022192083 A1 WO 2022192083A1
Authority
WO
WIPO (PCT)
Prior art keywords
polymer
oligonucleotides
substrate
region
moieties
Prior art date
Application number
PCT/US2022/018909
Other languages
English (en)
French (fr)
Inventor
Brian Mather
Weixian XI
Original Assignee
Illumina, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina, Inc. filed Critical Illumina, Inc.
Priority to CA3210142A priority Critical patent/CA3210142A1/en
Priority to AU2022232893A priority patent/AU2022232893A1/en
Priority to EP22712167.0A priority patent/EP4305193A1/en
Priority to CN202280019973.2A priority patent/CN117043354A/zh
Priority to JP2023552587A priority patent/JP2024515428A/ja
Priority to KR1020237031500A priority patent/KR20230157348A/ko
Publication of WO2022192083A1 publication Critical patent/WO2022192083A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00—Chemical modification by after-treatment
    • C08F8/30—Introducing nitrogen atoms or nitrogen-containing groups
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/56—Acrylamide; Methacrylamide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00—Chemical modification by after-treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/60—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing nitrogen in addition to the carbonamido nitrogen
    • C08F220/603—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing nitrogen in addition to the carbonamido nitrogen and containing oxygen in addition to the carbonamido oxygen and nitrogen
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L33/00—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
    • C08L33/04—Homopolymers or copolymers of esters
    • C08L33/06—Homopolymers or copolymers of esters of esters containing only carbon, hydrogen and oxygen, which oxygen atoms are present only as part of the carboxyl radical
    • C08L33/062—Copolymers with monomers not covered by C08L33/06
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L33/00—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
    • C08L33/24—Homopolymers or copolymers of amides or imides
    • C08L33/26—Homopolymers or copolymers of acrylamide or methacrylamide
PCT/US2022/018909 2021-03-09 2022-03-04 Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers WO2022192083A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA3210142A CA3210142A1 (en) 2021-03-09 2022-03-04 Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
AU2022232893A AU2022232893A1 (en) 2021-03-09 2022-03-04 Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
EP22712167.0A EP4305193A1 (en) 2021-03-09 2022-03-04 Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
CN202280019973.2A CN117043354A (zh) 2021-03-09 2022-03-04 聚合物、制备聚合物的方法以及使寡核苷酸与聚合物偶联的方法
JP2023552587A JP2024515428A (ja) 2021-03-09 2022-03-04 ポリマー、ポリマーを作製する方法、及びオリゴヌクレオチドをポリマーにカップリングする方法
KR1020237031500A KR20230157348A (ko) 2021-03-09 2022-03-04 중합체, 중합체의 제조 방법, 및 올리고뉴클레오티드를 중합체에 커플링하는 방법

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163158470P 2021-03-09 2021-03-09
US63/158,470 2021-03-09

Publications (1)

Publication Number Publication Date
WO2022192083A1 true WO2022192083A1 (en) 2022-09-15

Family

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Application Number Title Priority Date Filing Date
PCT/US2022/018909 WO2022192083A1 (en) 2021-03-09 2022-03-04 Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers

Country Status (8)

Country Link
US (1) US20220289876A1 (ko)
EP (1) EP4305193A1 (ko)
JP (1) JP2024515428A (ko)
KR (1) KR20230157348A (ko)
CN (1) CN117043354A (ko)
AU (1) AU2022232893A1 (ko)
CA (1) CA3210142A1 (ko)
WO (1) WO2022192083A1 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023010105A3 (en) * 2021-07-30 2023-03-09 Illumina, Inc. Light-activated coupling of oligonucleotides to polymers

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WO2006084482A1 (en) * 2005-02-10 2006-08-17 Commissariat A L'energie Atomique Method for the photochemical attachment of biomolecules to a substrate
US20120053299A1 (en) * 2009-02-19 2012-03-01 University Of Georgia Research Foundation, Inc. Cyclopropenones and the Photochemical Generation of Cyclic Alkynes Therefrom
WO2013184796A1 (en) * 2012-06-08 2013-12-12 Illumina, Inc. Polymer coatings
WO2015002813A1 (en) * 2013-07-01 2015-01-08 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
CN110055318A (zh) * 2019-05-06 2019-07-26 南京拓远生物科技有限公司 一种基于点击化学的三维dna微阵列表面的制备方法
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WO2021028815A1 (en) 2019-08-09 2021-02-18 Illumina Inc. System and method for patterning flow cell substrates

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Publication number Priority date Publication date Assignee Title
WO2006084482A1 (en) * 2005-02-10 2006-08-17 Commissariat A L'energie Atomique Method for the photochemical attachment of biomolecules to a substrate
US20120053299A1 (en) * 2009-02-19 2012-03-01 University Of Georgia Research Foundation, Inc. Cyclopropenones and the Photochemical Generation of Cyclic Alkynes Therefrom
WO2013184796A1 (en) * 2012-06-08 2013-12-12 Illumina, Inc. Polymer coatings
WO2015002813A1 (en) * 2013-07-01 2015-01-08 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
US9994687B2 (en) 2013-07-01 2018-06-12 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
WO2020097005A1 (en) * 2018-11-06 2020-05-14 Rutgers, The State University Of New Jersey Derivatizable monomers and polymers, and methods for making and using same
CN110055318A (zh) * 2019-05-06 2019-07-26 南京拓远生物科技有限公司 一种基于点击化学的三维dna微阵列表面的制备方法
WO2021028815A1 (en) 2019-08-09 2021-02-18 Illumina Inc. System and method for patterning flow cell substrates

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ARUMUGAM SELVANATHAN ET AL: "Photo-click chemistry strategies for spatiotemporal control of metal-free ligation, labeling, and surface derivatization", PURE & APPLIED CHEMISTRY, vol. 85, no. 7, 23 May 2013 (2013-05-23), GB, pages 1499 - 1513, XP055947986, ISSN: 0033-4545, DOI: 10.1351/PAC-CON-13-01-08 *
CHRISEY L. ET AL: "Fabrication of patterned DNA surfaces", NUCLEIC ACIDS RESEARCH, vol. 24, no. 15, 1 August 1996 (1996-08-01), pages 3040 - 3047, XP055922338, DOI: 10.1093/nar/24.15.3040 *
K. V. VOELKERDING ET AL: "Next-Generation Sequencing: From Basic Research to Diagnostics", CLINICAL CHEMISTRY, vol. 55, no. 4, 26 February 2009 (2009-02-26), pages 641 - 658, XP055057879, ISSN: 0009-9147, DOI: 10.1373/clinchem.2008.112789 *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023010105A3 (en) * 2021-07-30 2023-03-09 Illumina, Inc. Light-activated coupling of oligonucleotides to polymers

Also Published As

Publication number Publication date
KR20230157348A (ko) 2023-11-16
US20220289876A1 (en) 2022-09-15
EP4305193A1 (en) 2024-01-17
CA3210142A1 (en) 2022-09-15
CN117043354A (zh) 2023-11-10
AU2022232893A1 (en) 2023-09-14
JP2024515428A (ja) 2024-04-10

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