WO2022180446A1

WO2022180446A1 - Disinfection agent

Info

Publication number: WO2022180446A1
Application number: PCT/IB2021/062318
Authority: WO
Inventors: Григорий БУБНОВ; Виктор СВИСТУНОВ
Original assignee: Григорий БУБНОВ
Priority date: 2021-02-28
Filing date: 2021-12-26
Publication date: 2022-09-01
Also published as: RU2757361C1

Abstract

The proposed invention relates to chemical non-toxic antibacterial, antiviral, and antifungal preparations and is intended for disinfecting water and treating food products and any surfaces and objects, including the skin and hair of humans and animals, medical and household appliances, etc. The preparation can be used in medicine, agriculture, the food industry, sanitation, everyday life, and many other areas of human activity. The preparation comprises hypochlorous acid, which is dynamically equilibrated with sodium hypochlorite and is a source of active chlorine; hydrogen peroxide; water and sodium chloride. The preparation is safe for humans and the environment. The achieved technical result consists in creating an effective disinfectant based on compounds which are a source of active chlorine in a lower concentration compared to hypochlorites; and hydrogen peroxide.

Description

DISINFECTANT

The field of technology to which the invention belongs.

The invention relates to disinfectants containing hypochlorous acid in equilibrium with sodium hypochlorite and can be used for water disinfection, food processing, any surfaces and objects, including human and animal skin and hair, medical and household appliances, etc. e. That is, the claimed invention relates to chemical non-toxic antibacterial, antiviral and antifungal drugs containing active chlorine, and is intended for water disinfection, food processing, any surfaces and objects, including human and animal skin and hair, medical and household devices, etc. The drug can be widely used in medicine, agriculture, food industry, sanitation, everyday life and many other areas of human activity.

The level of technology.

The most traditional chlorine-containing disinfectants are hypochlorites and chloramines [B. I. Vashkov. Means and methods of sterilization used in medicine. M., "Medicine", 1975, p. 176 - 178].

Chloramines are less effective disinfectants than hypochlorites. Chloramines break down slowly. They cannot be removed by boiling, distillation or settling. Exposure to chloramines causes damage to the mucous membrane of the lungs, serious skin reactions, burning, redness and dryness of the eyes, gastrointestinal problems [A.A. Furman. Chlorine-containing oxidizing-bleaching and disinfecting agents. M., "Chemistry", 1976]. Sodium and calcium hypochlorites containing active chlorine are widely used as antibacterial disinfectants [Hypochlorites // Chemical Encyclopedia / Chief editor IL Knunyants. - M.: Soviet Encyclopedia, 1988. - T. 1. - S. 1121 - 1122]. However, in High concentrations of hypochlorites can have harmful effects on the human body. Hypochlorite solutions can be hazardous by inhalation due to the release of toxic chlorine, which causes irritant and asphyxiant effects. The content of active chlorine in hypochlorites ranges from 17 to 95%. [Harmful substances in industry. Handbook for chemists, engineers and doctors / Ed. prof. N. V. Lazareva and prof. I. D. Gadaskina. — Edition 7e, trans. and additional - L .: Chemistry, 1977. - T. 3. - S. 44].

The closest in technical essence and purpose to the claimed preparation is a disinfectant solution obtained in the process of anodic electrochemical activation of a 0.1-0.5% aqueous solution of sodium chloride, which, being an anolyte with acidic or close to neutral properties (pH from 2, 0 to 7.5), has oxidizing activity and is used mainly at redox potential (ORP) values of 600-800 mV in the concentration range of active chlorine ions Ca.x.200-400 mg/l, which leads to high biocidal activity. The disadvantages of the known means include the presence of a pungent odor and a significant decrease in functional activity during storage for more than 1-2 days as a result of spontaneous decomposition of highly active compounds and particles [B. .Bahir. electrochemical activation. Moscow: VNIIIMT. 1992, pp. 294-296, 312-314].

Disclosure of the invention.

Based on this original observation, the present invention mainly aims to provide a disinfectant containing hypochlorous acid in equilibrium with sodium hypochlorite, which makes it possible to create an effective disinfectant based on compounds that are a source of active chlorine at a lower concentration compared to hypochlorites. , and hydrogen peroxide, which is the task.

To achieve this goal, the disinfectant also contains hydrogen peroxide, in the following quantitative ratio, wt. %:

• hypochlorous acid in equilibrium with sodium hypochlorite - 0.02-15.0 • sodium chloride - 5.0-10.0

• hydrogen peroxide - 0, 1 -30.0

• water - the rest.

Thanks to these advantageous characteristics, it becomes possible to create an effective disinfectant based on compounds that are a source of active chlorine in a lower concentration compared to hypochlorites and hydrogen peroxide, as well as to increase the degree of disinfection.

The ranges were chosen for the following reasons:

- if hypochlorous acid in equilibrium with sodium hypochlorite is less than 0.05% in solution, then it was found from the tests that the disinfecting effect disappears.

- solutions of hypochlorous acid (in equilibrium with sodium hypochlorite) in a concentration of more than 30% do not exist,

- optimal concentration of hypochlorous acid in equilibrium with sodium hypochlorite 5%;

- the concentration of sodium chloride in the solution is chosen from considerations of how much it remains after electrolysis;

- if hydrogen peroxide is less than 0.1% in solution, then it was found from the tests that the disinfecting effect disappears.

- if hydrogen peroxide is more than 30% in solution, then in these high concentrations (30% or more) the solution has a local irritating effect and causes depigmentation of the skin and mucous membranes. (Source: https://6M3.opr/index.php/nEPEKHCb HYDROGEN) - The optimal concentration of hydrogen peroxide is 2-5%.

There is a variant of the invention, in which the disinfectant also contains alcohol in the following proportion, wt. %: 0.1-20.0.

Due to these advantageous characteristics, the disinfectant properties of the agent are further enhanced due to the fact that alcohol is also a protein-degrading agent. Brief description of the drawings.

Other features and advantages of the present invention will be clear from the description which follows, by way of illustration and without being restrictive, with reference to the accompanying drawings, in which:

- figure 1 schematically depicts the tests carried out in Petri dishes, according to the invention.

Implementation of the invention.

The inventive disinfectant can be obtained from an aqueous solution of sodium chloride with a concentration of 5 to 300 g / l (0.5 - 30%) for electrochemical electrolysis in an EL-400 unit with a power of 600 W, powered by an alternating current of 220/380 V, 50 Hz, giving a performance of a solution containing active chlorine, 40 l / h. The content of other mineral substances in water should not exceed 200 mg/l.

To the resulting solution is added hydrogen peroxide, conventional [GOST 177-88] or stabilized [GOST R 50632-93] to a total concentration of 0.1 to 30%.

The claimed invention is a colorless transparent liquid with a slight smell of chlorine, pH = 7.7 ± 0.5, boiling point 100°C. The drug is miscible with water in any ratio and is safe for humans and the environment.

Industrial applicability.

The disinfectant can be implemented by a specialist in practice and in the implementation ensure the implementation of the stated purpose, which allows us to conclude that the criterion of "industrial applicability" for the invention is met.

In accordance with the proposed invention, the proposed disinfectant was tested.

The action of the agent according to the proposed claims was tested: • hypochlorous acid in equilibrium with sodium hypochlorite - 5.00 - 10.0 - 5.0 (three options)

• sodium chloride - 1.0

• hydrogen peroxide - 2.0

• water - the rest.

The comparison was made with a solution that did not include hydrogen peroxide and consisted of:

• hypochlorous acid in equilibrium with sodium hypochlorite - 5.00 - 10.0 - 15.0 (three options)

• sodium chloride - 1.0

• water - the rest.

The claimed invention was tested by disk diffusion method for antimicrobial and antifungal activity against the following microorganisms: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Candida albicans. As a control sample, a solution was taken containing active chlorine at a concentration of 0.5%, without the addition of hydrogen peroxide.

The control solution showed only weak antifungal activity. The claimed invention exhibits significant antimicrobial and antifungal activity, which reaches a maximum at a hydrogen peroxide concentration of 2.0% and does not change with increasing concentration.

The tests were carried out by the testing laboratory of the Institute of Biochemistry and Microbiology Testing Laboratory N° 1316.3. Test date: 09 - 13.11.2020

Supplied (tested) disinfectant solutions: Disinfectant solutions with an increasing concentration of the active substance - hypochlorous acid in equilibrium with sodium hypochlorite:

125S conc. 5% (top row of Petri dishes in figure 1)

126S conc. 10% (middle row of Petri dishes in figure 1)

127S conc. 15% (bottom row of Petri dishes in figure 1)

Test microorganisms were used for testing:

Staphylococcus aureus STA ATCC 25923

Escherichia coli EC ATCC 8739 Pseudomonas aeruginosa PSA ATCC 27853

Bacillus cereus BC ATCC 19637

Candida albicans CA ATCC 10231

In figure 1, these are sequentially vertical columns.

Method used: The solid media diffusion method was chosen in accordance with the international CLSI standard to test the antimicrobial activity of the delivered samples. In this technique, the seeding method and density are prescribed.

seed material.

Suspensions with a density of 0.5 μF were prepared from the tested microorganisms in physiological saline. They were sown on pre-dried Petri dishes with nutrient medium. The distribution was carried out evenly over the entire surface of the cup with a sterile spatula in 3 directions.

Holes with a diameter of 0.5 cm were formed on separate dishes prepared in this way.

Cultivation took place in thermostats at the following temperatures:

Bacteria (St.aureus, E.coli, Psaeruginosa) - > 37 ° C approximately 24 hours

Bacteria (Bacillus cereus) - > 30 ° C approximately 24 hours

Yeast (Candida albicans) - > 25 ° C approx. 72 hours

At the end of the cultivation period, the sizes of the zones of inhibition were evaluated.

Conclusion: The tested disinfectant solutions showed antibacterial activity at all tested concentrations of hypochlorous acid in equilibrium with sodium hypochlorite. Disinfectant solutions at concentrations of 10 and 15% had antibacterial activity almost identical to a 5% solution for St. aureus and Ps. aeruginosa. E. coli sporulating Bacillus cereus and Candida albicans showed lower efficacy at all three concentrations.

All this shows a significant increase in the effectiveness of disinfection when using the disinfectant of the present invention. Thus, in this invention, the task was achieved - the creation of an effective disinfectant based on compounds that are a source of active chlorine in a lower concentration compared to hypochlorites, and hydrogen peroxide.

Claims

CLAIM

1. Disinfectant containing hypochlorous acid in equilibrium with sodium hypochlorite, characterized in that it also contains hydrogen peroxide, in the following ratio, wt. %: hypochlorous acid in equilibrium with sodium hypochlorite - 0.02-15.0 sodium chloride - 5.0-10.0 hydrogen peroxide - 0.1-30.0 water - the rest.

2. Disinfectant, according to claim 1, characterized in that it additionally contains alcohol, wt. %: 0.1-20.0.