RU2757361C1 - Desinfection agent - Google Patents

Abstract

FIELD: disinfectants.SUBSTANCE: invention relates to disinfectants and can be used for disinfection of water, food processing, any surfaces and objects. The disinfectant contains hypochlorous acid in equilibrium with sodium hypochlorite, sodium chloride and hydrogen peroxide, in the following ratio, by weight. %: hypochlorous acid in equilibrium with sodium hypochlorite - 0.02-15.0; sodium chloride - 5.0-10.0; hydrogen peroxide - 0.1-30.0; water - the rest. The agent is safe for humans and the environment.EFFECT: invention provides for creation of an effective disinfectant based on compounds that are a source of active chlorine in a lower concentration compared to hypochlorites, and hydrogen peroxide.2 cl, 1 dwg

Description

The technical field to which the invention relates

The invention relates to disinfectants containing hypochlorous acid in equilibrium with sodium hypochlorite and can be used for water disinfection, food processing, any surfaces and objects, including human and animal skin and hair, medical and household appliances, etc. That is, the claimed invention relates to chemical non-toxic antibacterial, antiviral and antifungal drugs containing active chlorine, and is intended for water disinfection, food processing, any surfaces and objects, including the skin and hair of humans and animals, medical and household devices, etc. The drug can be widely used in medicine, agriculture, food industry, sanitation, everyday life and many other areas of human activity.

State of the art

The most traditional chlorine-containing disinfectants are hypochlorites and chloramines [V. I. Vashkov. Means and methods of sterilization used in medicine. M., "Medicine", 1975, p. 176 - 178].

Chloramines are less effective disinfectants than hypochlorites. Chloramines disintegrate slowly. They cannot be removed by boiling, distillation or settling. Exposure to chloramines causes damage to the lung mucosa, severe skin reactions, burning, redness and dryness of the eyes, gastrointestinal problems [A.A. Furman. Chlorine-containing oxidizing-bleaching and disinfecting substances. M., "Chemistry", 1976].

Sodium and calcium hypochlorites containing active chlorine are widely used as antibacterial disinfectants [Hypochlorites // Chemical encyclopedia / Editor-in-chief IL Knunyants. - M .: Soviet encyclopedia, 1988. - T. 1. - S. 1121-1122]. However, in high concentrations, hypochlorites can have a harmful effect on the human body. Hypochlorite solutions can be hazardous by inhalation due to the release of toxic chlorine, which causes irritation and asphyxiation. The content of active chlorine in hypochlorites ranges from 17 to 95%. [Harmful substances in industry. Handbook for chemists, engineers and doctors / Ed. prof. N.V. Lazareva and prof. I. D. Gadaskina. - Edition 7e, per. and add. - L .: Chemistry, 1977. - T. 3. - S. 44].

The closest in technical essence and purpose to the claimed drug is a disinfectant solution obtained in the process of anodic electrochemical activation of a 0.1-0.5% aqueous solution of sodium chloride, which is an anolyte with acidic or close to neutral properties (pH from 2, 0 to 7.5), has oxidative activity and is used mainly at values of the redox potential (ORP) 600-800 mV in the concentration range of active chlorine ions C _a.x. 200-400 mg / l, which leads to high biocidal activity. The disadvantages of the known agent include the presence of a pungent odor and a significant decrease in functional activity during storage for more than 1-2 days as a result of spontaneous disintegration of highly active compounds and particles [VM Bakhir. Electrochemical activation. M .: VNIIIMT. 1992, pp. 294-296, 312-314].

Disclosure of invention

Based on this original observation, the present invention is mainly intended to provide a disinfectant containing hypochlorous acid in equilibrium with sodium hypochlorite, allowing an effective disinfectant based on compounds that are a source of active chlorine in a concentration lower than hypochlorites. , and hydrogen peroxide, which is the task at hand.

To achieve this goal, the disinfectant also contains hydrogen peroxide, in the following quantitative ratio, wt. %:

hypochlorous acid in equilibrium with sodium hypochlorite - 0.02-15.0

sodium chloride - 5.0-10.0

hydrogen peroxide - 0.1-30.0

water - the rest

Thanks to these advantageous characteristics, it becomes possible to create an effective disinfectant based on compounds that are a source of active chlorine in a lower concentration than hypochlorites, and hydrogen peroxide, as well as to provide an increase in the degree of disinfection.

The ranges were chosen for the following reasons:

- if hypochlorous acid in equilibrium with sodium hypochlorite is less than 0.05% in solution, then it was found from tests that the disinfecting effect disappears.

- solutions of hypochlorous acid (in equilibrium with sodium hypochlorite) at a concentration of more than 30% do not exist,

- the optimal concentration of hypochlorous acid in equilibrium with sodium hypochlorite 5%;

- the concentration of sodium chloride in the solution is selected from considerations of how much of it remains after electrolysis;

- if hydrogen peroxide is less than 0.1% in solution, then it was found from the tests that the disinfecting effect disappears.

- if hydrogen peroxide is more than 30% in the solution, then at these high concentrations (30% or more) the solution has a local irritating effect and causes depigmentation of the skin and mucous membranes. (Source: https: //bme.org/index.php/ HYDROGEN_PEROXIDE) - the optimal concentration of hydrogen peroxide is 2-5%.

There is a variant of the invention, in which the disinfectant also contains alcohol in the following quantitative ratio, wt. %: 0.1-20.0.

Thanks to these advantageous characteristics, the disinfectant properties of the agent are additionally increased due to the fact that alcohol is also a protein degrading agent.

Brief Description of Drawings

Other distinctive features and advantages of the present invention clearly follow from the description given below by way of illustration and not limiting, with reference to the accompanying drawings, in which:

- figure 1 schematically depicts the tests carried out in petri dishes according to the invention.

Implementation of the invention

The claimed disinfectant can be obtained from an aqueous solution of sodium chloride with a concentration of 5 to 300 g / l (0.5 - 30%) for electrochemical electrolysis in an EL-400 installation with a power of 600 W, operating from an alternating current network of 220/380 V, 50 Hz, giving performance for a solution containing active chlorine, 40 l / h. The content of other minerals in water should not exceed 200 mg / l.

To the resulting solution is added hydrogen peroxide, ordinary [GOST 177-88] or stabilized [GOST R 50632-93] to a total concentration of 0.1 to 30%.

The claimed invention is a colorless transparent liquid with a weak chlorine odor, pH 7.7 ± 0.5, boiling point 100 ° C. The drug is miscible with water in any way and is safe for humans and the environment.

Industrial applicability

The means for disinfection can be carried out by a specialist in practice and, when implemented, ensure the realization of the declared purpose, which makes it possible to conclude that the criterion of "industrial applicability" for the invention is met.

In accordance with the proposed invention, the proposed disinfectant was tested.

The action of the agent was tested according to the proposed claims:

hypochlorous acid in equilibrium with sodium hypochlorite - 5.00 - 10.0 - 15.0 (three options)

sodium chloride - 1.0

hydrogen peroxide - 2.0

water - the rest

The comparison was made with a solution that did not include hydrogen peroxide and consisted of:

hypochlorous acid in equilibrium with sodium hypochlorite - 5.00 - 10.0 - 15.0 (three options)

sodium chloride - 1.0

water - the rest

The claimed invention was tested by the disk-diffusion method for antimicrobial and antifungal activity against the following microorganisms: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Candida albicans. A solution containing active chlorine at a concentration of 0.5% was taken as a control sample, without the addition of hydrogen peroxide.

The control solution showed only weak antifungal activity. The claimed invention exhibits significant antimicrobial and antifungal activity, which reaches a maximum at a hydrogen peroxide concentration of 2.0% and does not change with increasing concentration.

The tests were carried out by the testing laboratory of the Institute of Biochemistry and Microbiology Testing laboratory No. 1316.3. Testing date: 09 - 13.11.2020.

Supplied (tested) disinfectant solutions: Disinfectant solutions with an increasing concentration of the active substance - hypochlorous acid in equilibrium with sodium hypochlorite:

125S conc. 5% (top row of Petri dishes in Fig. 1)

126S conc. 10% (middle row of Petri dishes in Fig. 1)

127S conc. 15% (bottom row of Petri dishes in Fig. 1)

For verification, test microorganisms were used:

Staphylococcus aureus STA ATCC 25923 Escherichia coli EC ATCC 8739 Pseudomonas aeruginosa PSA ATCC 27853 Bacillus cereus BC ATCC 19637 Candida albicans CA ATCC 10231

In Figure 1, these are successively vertical columns.

Method used: The solid medium diffusion method was selected in accordance with the international CLSI standard to test the antimicrobial activity of the delivered samples. In this technique, the seeding method and density are prescribed.

Seed material

Suspensions with a density of 0.5 μF were prepared from the tested microorganisms in saline. They were plated on pre-dried Petri dishes with a nutrient medium. Distribution was made evenly over the entire surface of the cup with a sterile spatula in 3 directions.

A hole with a diameter of 0.5 cm was formed on the individual dishes prepared in this way. In each of them, 70 μl of the sample of the disinfectant solution under study was pipetted.

Cultivation took place in thermostats at the following temperatures:

Bacteria (St. aureus, E. coli, Psaeruginosa) → 37 ° C for about 24 hours

Bacteria (Bacillus cereus) → 30 ° C for about 24 hours

Yeast (Candida albicans) → 25 ° C approx. 72 h

At the end of the cultivation period, the sizes of the zones of inhibition were evaluated.

Conclusion: the tested disinfectant solutions showed antibacterial activity at all tested concentrations of hypochlorous acid in equilibrium with sodium hypochlorite. Disinfectant solutions at concentrations of 10 and 15% had antibacterial activity, almost identical to the 5% solution for St. aureus and Ps.aeruginosa. E. coli sporulating Bacillus cereus and Candida albicans showed lower efficacy at all three concentrations.

All of this shows a significant increase in disinfection efficiency when using the disinfectant of the present invention.

Thus, this invention has achieved the task set - the creation of an effective disinfectant based on compounds that are a source of active chlorine in a lower concentration than hypochlorites, and hydrogen peroxide.

Claims (3)

1. A disinfectant containing hypochlorous acid in equilibrium with sodium hypochlorite, characterized in that it also contains hydrogen peroxide, in the following quantitative ratio, wt. %: hypochlorous acid in equilibrium with sodium hypochlorite 0.02-15.0 sodium chloride 5.0-10.0 hydrogen peroxide 0.1-30.0 water rest 2. Means for disinfection, under item 1, characterized in that it additionally contains alcohol, mass. %: 0.1-20.0.

Images