CA2987632A1 - Biocidal composition comprising electrolyzed water and an amine - Google Patents
Biocidal composition comprising electrolyzed water and an amine Download PDFInfo
- Publication number
- CA2987632A1 CA2987632A1 CA2987632A CA2987632A CA2987632A1 CA 2987632 A1 CA2987632 A1 CA 2987632A1 CA 2987632 A CA2987632 A CA 2987632A CA 2987632 A CA2987632 A CA 2987632A CA 2987632 A1 CA2987632 A1 CA 2987632A1
- Authority
- CA
- Canada
- Prior art keywords
- biocidal composition
- amine
- electrolyzed water
- water
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000000203 mixture Substances 0.000 title claims abstract description 68
- 230000003115 biocidal effect Effects 0.000 title claims abstract description 62
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 57
- 150000001412 amines Chemical class 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 26
- 244000005700 microbiome Species 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 12
- 241000233866 Fungi Species 0.000 claims abstract description 12
- 241000700605 Viruses Species 0.000 claims abstract description 7
- 241000195493 Cryptophyta Species 0.000 claims abstract description 5
- 239000000047 product Substances 0.000 claims description 17
- 239000007795 chemical reaction product Substances 0.000 claims description 15
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 239000002689 soil Substances 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 230000000813 microbial effect Effects 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 241000193403 Clostridium Species 0.000 claims description 3
- 241000186781 Listeria Species 0.000 claims description 3
- 241000191940 Staphylococcus Species 0.000 claims description 3
- 241000194017 Streptococcus Species 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 241000604933 Bdellovibrio Species 0.000 claims description 2
- 241000191368 Chlorobi Species 0.000 claims description 2
- 241001142109 Chloroflexi Species 0.000 claims description 2
- 241000192700 Cyanobacteria Species 0.000 claims description 2
- 241000605716 Desulfovibrio Species 0.000 claims description 2
- 241000588921 Enterobacteriaceae Species 0.000 claims description 2
- 241000588722 Escherichia Species 0.000 claims description 2
- 241000192125 Firmicutes Species 0.000 claims description 2
- 241000589989 Helicobacter Species 0.000 claims description 2
- 241000589248 Legionella Species 0.000 claims description 2
- 208000007764 Legionnaires' Disease Diseases 0.000 claims description 2
- 241000588621 Moraxella Species 0.000 claims description 2
- 241000589516 Pseudomonas Species 0.000 claims description 2
- 241000607142 Salmonella Species 0.000 claims description 2
- 241000607768 Shigella Species 0.000 claims description 2
- 241001180364 Spirochaetes Species 0.000 claims description 2
- 241000122971 Stenotrophomonas Species 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 2
- 230000002147 killing effect Effects 0.000 claims description 2
- 244000052769 pathogen Species 0.000 claims description 2
- 230000001717 pathogenic effect Effects 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 239000000460 chlorine Substances 0.000 description 21
- 229910052801 chlorine Inorganic materials 0.000 description 21
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 20
- 239000003139 biocide Substances 0.000 description 20
- 239000000243 solution Substances 0.000 description 10
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 8
- 239000000645 desinfectant Substances 0.000 description 8
- 230000000284 resting effect Effects 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 239000006150 trypticase soy agar Substances 0.000 description 7
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 6
- 239000005708 Sodium hypochlorite Substances 0.000 description 6
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 6
- 239000008188 pellet Substances 0.000 description 6
- 239000003755 preservative agent Substances 0.000 description 6
- 230000002335 preservative effect Effects 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 238000009792 diffusion process Methods 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 238000005868 electrolysis reaction Methods 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 230000001590 oxidative effect Effects 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 4
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 description 4
- -1 pharmaceutical Substances 0.000 description 4
- 239000001103 potassium chloride Substances 0.000 description 4
- 235000011164 potassium chloride Nutrition 0.000 description 4
- 239000000376 reactant Substances 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000007844 bleaching agent Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 229920006395 saturated elastomer Polymers 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- COXVTLYNGOIATD-HVMBLDELSA-N CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O Chemical compound CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O COXVTLYNGOIATD-HVMBLDELSA-N 0.000 description 2
- XTEGARKTQYYJKE-UHFFFAOYSA-M Chlorate Chemical compound [O-]Cl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-M 0.000 description 2
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 2
- 239000004155 Chlorine dioxide Substances 0.000 description 2
- 241000991587 Enterovirus C Species 0.000 description 2
- QUSNBJAOOMFDIB-UHFFFAOYSA-N Ethylamine Chemical compound CCN QUSNBJAOOMFDIB-UHFFFAOYSA-N 0.000 description 2
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- WGQKYBSKWIADBV-UHFFFAOYSA-N benzylamine Chemical compound NCC1=CC=CC=C1 WGQKYBSKWIADBV-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 235000019398 chlorine dioxide Nutrition 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000013068 control sample Substances 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 2
- 229960003699 evans blue Drugs 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000009422 growth inhibiting effect Effects 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Inorganic materials Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000003641 microbiacidal effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002417 nutraceutical Substances 0.000 description 2
- 235000021436 nutraceutical agent Nutrition 0.000 description 2
- 125000000962 organic group Chemical group 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 239000012064 sodium phosphate buffer Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 2
- 244000303769 Amaranthus cruentus Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000000385 Brassica napus var. napus Species 0.000 description 1
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 235000005087 Malus prunifolia Nutrition 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- 241001503436 Plasmodiophora brassicae Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241000233639 Pythium Species 0.000 description 1
- 241000918585 Pythium aphanidermatum Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000193998 Streptococcus pneumoniae Species 0.000 description 1
- 241001286670 Ulmus x hollandica Species 0.000 description 1
- 241000228452 Venturia inaequalis Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 239000003619 algicide Substances 0.000 description 1
- 125000002723 alicyclic group Chemical group 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 150000001414 amino alcohols Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000001098 anti-algal effect Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003190 augmentative effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 229910001919 chlorite Inorganic materials 0.000 description 1
- 229910052619 chlorite group Inorganic materials 0.000 description 1
- QBWCMBCROVPCKQ-UHFFFAOYSA-N chlorous acid Chemical compound OCl=O QBWCMBCROVPCKQ-UHFFFAOYSA-N 0.000 description 1
- 239000013066 combination product Substances 0.000 description 1
- 229940127555 combination product Drugs 0.000 description 1
- 238000012504 compendial method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 239000007799 cork Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical compound CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 229940083124 ganglion-blocking antiadrenergic secondary and tertiary amines Drugs 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 238000002483 medication Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 230000009972 noncorrosive effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003856 quaternary ammonium compounds Chemical class 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000011272 standard treatment Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229940031000 streptococcus pneumoniae Drugs 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 210000000605 viral structure Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/08—Amines; Quaternary ammonium compounds containing oxygen or sulfur
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/03—Electric current
- A61L2/035—Electrolysis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
- A61L2/06—Hot gas
- A61L2/07—Steam
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/23—Containers, e.g. vials, bottles, syringes, mail
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention is directed to a biocidal composition formed by a reaction or combination of electrolyzed water and an amine, and methods for using same to kill or control microorganisms, such as viruses, bacteria, fungi and algae.
Description
BIOCIDE COMPOSITION AND METHODS OF USE
INVENTORS: STOREY, William Dale ARRISON, Norman L.
Field of the Invention [0001] The present invention is directed to a composition comprising the reaction products of electrolyzed water and an amine, and biocidal uses of the same.
Background of the Invention
INVENTORS: STOREY, William Dale ARRISON, Norman L.
Field of the Invention [0001] The present invention is directed to a composition comprising the reaction products of electrolyzed water and an amine, and biocidal uses of the same.
Background of the Invention
[0002] Disinfectants or biocides are widely used in healthcare, industrial or household environments. Commonly used biocides include oxidizing biocides, alcohols, aldehydes, and surfactants.
[0003] Oxidizing biocides are general chemical oxidants. They are not selective for living organisms, but react with any oxidizable matter. However, they are microbiocidal because certain bacterial cell and viral components can react readily with them, having a higher oxidation potential than most other chemicals present in water. Three classes of oxidizing biocides are generally available for microbiocidal applications; oxidizing halogens, peroxides and ozone.
[0004] As an example of an oxidizing halogen, household bleach is commonly used as a disinfectant. Bleach is a solution of sodium hypochlorite, which interconverts with other chemical forms of free chlorine, mainly hypochlorous acid. Hypochlorous acid is believed to denature microbial proteins to kill bacteria and viruses. Another source of hypochlorous acid is electrolyzed water (also known as electrochemically activated water or anolyte) which is known to have biocidal properties due to its content of free chlorine.
[0005] Some surfactants are known to have biocidal activities. For example, quaternary ammonium compounds are non-corrosive and stable and widely used as a disinfectant.
[0006] There remains a need for biocidal agents which can be used in therapeutic, industrial or agricultural applications, which preferably have broad-spectrum activity and have limited side-effects or toxicity.
Summary of the Invention
Summary of the Invention
[0007] The present invention is directed a biocidal composition which comprises the reaction products of electrolyzed water and an amine, and methods for using the same in therapeutic, industrial or agricultural applications.
[0008] In one aspect, the invention may comprise a method of killing or controlling microorganisms, comprising the step of contacting the microorganism with a composition comprising a reaction product of electrolyzed water and an amine. The microorganism may comprise Gram negative bacteria, excluding sulfate reducing species such as Desulfovibrio species, Gram postive bacteria such as Bacillus, Corynebacteria, Clostridium, Listeria, Staphylococcus or Streptococcus species. The microorganism may comprise a virus, a fungi such as plant pathogen such as a clubroot pathogen, or an algae.
[0009] In another aspect, the invention may comprise a method of controlling a plant disease caused by a fungus, comprising contacting plant seeds, soil or other growth medium with a biocidal composition comprising a reaction product of electrolyzed water and an amine.
[00010] In another aspect, the invention may comprise a method of disinfecting a surface comprising the step of applying the biocidal composition comprising a reaction product of electrolyzed water and an amine to the surface. The biocidal composition may be heated, and in one embodiment, may be mixed with water and heated to form steam.
[00011] In another aspect, the invention may comprise a method of sanitizing a volume of fluid comprising the step of mixing the biocidal composition comprising a reaction product of electrolyzed water and an amine with the volume of fluid.
[00012] In another aspect, the invention may comprise a method of preserving a product susceptible to microbial spoilage, comprising applying a biocidal composition comprising a reaction product of electrolyzed water and an amine to the product. The product may comprise a cosmetic, pharmaceutical, nutraceutical, or an edible product intended for human or animal consumption.
Detailed Description of the Drawings
Detailed Description of the Drawings
[00013] Figure 1 shows a graph showing efficacy of a biocidal composition described herein in inactivating clubroot spores.
[00014] Figure 2 shows antibacterial efficacy of a biocidal composition described herein using both well diffusion and disk diffusion growth inhibition tests on agar plates.
Detailed Description of Preferred Embodiments
Detailed Description of Preferred Embodiments
[00015] In one embodiment, the invention provides a biocidal composition having a broad spectrum of activity and may be effective to kill or limit the growth of microorganisms including bacteria, viruses, or fungi.
[00016] In one embodiment, the biocidal composition may be mixed or diluted with water, which solution is then heated and used to steam-clean an article or an environment.
The steam itself can have a sterilizing effect which may be enhanced or augmented by the biocidal composition. In particular, when the steam condenses on a surface, the biocide may be thus be deposited on the surface and remain active.
Electrolyzed water is also known as electro-activated water or electro-chemically activated water solution. It is produced by the electrolysis of ordinary water containing dissolved sodium or potassium chloride. In one embodiment, the concentration of the sodium chloride or potassium chloride is adjusted to provide a chloride concentration in the range of between about 100 ppm to about 8000 ppm.
In a preferred embodiment, the concentration of the chloride ion is about 5500 ppm, or equivalent to about 9.08 g/litre of NaCI.
The steam itself can have a sterilizing effect which may be enhanced or augmented by the biocidal composition. In particular, when the steam condenses on a surface, the biocide may be thus be deposited on the surface and remain active.
Electrolyzed water is also known as electro-activated water or electro-chemically activated water solution. It is produced by the electrolysis of ordinary water containing dissolved sodium or potassium chloride. In one embodiment, the concentration of the sodium chloride or potassium chloride is adjusted to provide a chloride concentration in the range of between about 100 ppm to about 8000 ppm.
In a preferred embodiment, the concentration of the chloride ion is about 5500 ppm, or equivalent to about 9.08 g/litre of NaCI.
[00017] As used herein, "anolyte" means an aqueous solution produced at the anode by the electrolysis of aqueous solutions of sodium or potassium chloride. The anolyte produced comprises free available chlorine, primarily in the form of sodium hypochlorite. In one embodiment, the anolyte comprises greater than about 8000 ppm of free available chlorine. As one skilled in the art will appreciate, the level of free chlorine is related to the amount of chlorine ions provided in the electrolyzed water. The amount of free chlorine in a sample of anolyte is known to slowly decrease over time. "Catholyte" is the aqueous solution which is produced at the cathode.
[00018] As used herein, "electrolyzed water" means any aqueous solution which contains free available chlorine resulting from the electrolysis of water having dissolved chloride ions. It may include anolyte, or anolyte which has been mixed with catholyte. In one embodiment, the electrolyzed water comprises a mixture of equal volumes of anolyte and catholyte produced from the same electrolysis.
[00019] To prepare the biocidal composition of the present invention, suitable solutions of anolyte and catholyte may be produced by an electrolysis reactor, such as that described, for example, in US
Patent No. 4,875,988 to Aragon, issued October 24, 1989; US Patent No.
5,540,819 to Bakhir et al., issued July 30, 1996; US Patent No. 5,628,888 to Bakhir et at., issued May 13, 1997; and US Patent Application No. 12/962,385, filed December 7, 2010 (the entire contents of which are incorporated herein by reference for all purposes, where permitted). If the anode and cathode compartments are separated by a semi-permeable membrane, the composition of the anolyte and catholyte may be quite different. Typically, the anolyte is pH neutral or slightly acidic, while catholyte is basic due to the production of sodium hydroxide.
Patent No. 4,875,988 to Aragon, issued October 24, 1989; US Patent No.
5,540,819 to Bakhir et al., issued July 30, 1996; US Patent No. 5,628,888 to Bakhir et at., issued May 13, 1997; and US Patent Application No. 12/962,385, filed December 7, 2010 (the entire contents of which are incorporated herein by reference for all purposes, where permitted). If the anode and cathode compartments are separated by a semi-permeable membrane, the composition of the anolyte and catholyte may be quite different. Typically, the anolyte is pH neutral or slightly acidic, while catholyte is basic due to the production of sodium hydroxide.
[00020] Without restriction to a theory, it is believed that anolyte does not contain chlorite (C102-) and chlorate (CI03-) ions. The free available chlorine in anolyte exists primarily as hypochlorous acid (HC10) or hypochlorite ions (C10-), free chlorine (Cl2) and chloride ion (Cl-) and chlorine dioxide (CI02). Without restriction to a theory, it is believed that hypochlorous acid and hypochlorite ions, free chlorine, and/or chloride ions, are restricted or limited in crossing the ionomeric semi-permeable membrane. As a result, they may accumulate in the anolyte to very high levels.
[00021] In one embodiment, the anolyte is combined with the catholyte (which comprises primarily sodium or potassium hydroxide) produced at the cathode in the electrolytic process. The resulting solution still maintains a high concentration of free available chlorine and may have a pH of about 8.3.
Without restriction to a theory, it is believed that the majority of the free available chlorine exists as NaHCIO (sodium hypochlorite). In one embodiment, the concentration of free available chlorine is greater than about 1000 ppm in the electrolyzed water, preferably greater than about 3000 ppm, and more preferably greater than about 5500 ppm. The concentration may be as high as about 8000 ppm.
Without restriction to a theory, it is believed that the majority of the free available chlorine exists as NaHCIO (sodium hypochlorite). In one embodiment, the concentration of free available chlorine is greater than about 1000 ppm in the electrolyzed water, preferably greater than about 3000 ppm, and more preferably greater than about 5500 ppm. The concentration may be as high as about 8000 ppm.
[00022] Amines may be produced through well known chemical syntheses, and are readily commercially available. As used herein, "amine" means an organic compound with one or more of the hydrogen atoms in ammonia replaced by organic groups. The term includes the three classes of amines dependent on the number of hydrogen atoms replaced, namely primary, secondary and tertiary amines.
The organic groups can include aliphatic, alicyclic or aromatic groups.
Examples of amines include, but are not limited to, monoethanolamine, methylamine, ethylamine, diethylamine, trimethylamine, aniline, benzylamine, and the like. In one embodiment, the amine comprises an amino alcohol such as monoethanolamine.
The organic groups can include aliphatic, alicyclic or aromatic groups.
Examples of amines include, but are not limited to, monoethanolamine, methylamine, ethylamine, diethylamine, trimethylamine, aniline, benzylamine, and the like. In one embodiment, the amine comprises an amino alcohol such as monoethanolamine.
[00023] The biocidal composition of the present invention is prepared by combining electrolyzed water with the amine to form a reactant mixture, which then undergoes exothermic reactions or combinations. Preparation of the biocidal composition should preferably be conducted in a well-ventilated area, due to the possibility of chlorine gas production. . In one embodiment, the pH of the resulting composition is in a range of about pH 11.0 or higher and may be in the range of about 12.6 to about 13.3. In one embodiment, the resulting composition does not have any detectable free available chlorine.
[00024] Once the reaction is complete, the resulting composition comprises a reaction product or combination product between at least one component of the electrolyzed water and the amine. Without restriction to a theory, it is believed that components of the reaction product combine or complex with chlorine in some manner. Without restriction to a theory, it is believed that any available chlorine is bound or otherwise rendered undetectable by the amine, or the reaction products of the composition.
[00025] It will be appreciated by those skilled in the art that the proportions of the individual components may be varied while still producing a biocidal effect of the composition to suit the treatment circumstances. In one embodiment, the proportion of the electrolyzed water to the amine solution is in an amount between about 2% to about 98% by volume based on the total volume of the reactant mixture. In a preferred embodiment, the electrolyzed water is present in an amount of about 40% to about 60% by volume based on the total volume of the biocidal composition. In one embodiment, amine is present in an amount between about 5% to about 95% by volume based on the total volume of the biocidal composition, and in a preferred embodiment, is present in an amount of about 40% by volume based on the total volume of the biocidal composition.
Water or an aqueous salt solution may be used to make up volume of the reactant mixture. For example, in one embodiment, the reactant mixture may comprise 15% electrolyzed water, 10% monoethanolamine and 75% water (v:v)
Water or an aqueous salt solution may be used to make up volume of the reactant mixture. For example, in one embodiment, the reactant mixture may comprise 15% electrolyzed water, 10% monoethanolamine and 75% water (v:v)
[00026] The biocidal composition may be used as mixed, or may be diluted in water, an aqueous salt solution, an alcohol, a suitable solvent or mixtures thereof. In one embodiment, the biocidal composition may be used diluted in water as a 2% (v:v) solution, with effective biocidal activity on hard surfaces. The biocidal composition may be effective to kill or limit the growth of Gram positive bacteria such as Bacillus, Corynebacteria, Clostridium, Listeria, Staphylococcus or Streptococcus species; Gram negative bacteria such as Salmonella, Shigella, Escherichia, Enterobacteriaceae, Pseudomonas, Moraxella, Helicobacter, Stenotrophomonas, Bdellovibrio, acetic acid bacteria, Legionella, cyanobacteria, spirochaetes, green sulfur, and green non-sulfur bacteria.
[00027] The biocidal composition may be used in a variety of different manners. In industrial applications, it may be used to wash and disinfect various surfaces or containers. In one embodiment, the biocidal composition may be used to clean or treat sewage holding tanks, such as waste tanks for recreational vehicles or boats. Discharge or disposal of the composition after a cleaning treatment may be directly into the environment as the material has been demonstrated to be non-toxic.
[00028] The biocidal composition may be effective in a wide range of temperatures, such as from about from -500 to 250 C, and as such is effective when used in many industrial processes. In particular, the composition may be effectively used in conjuction with steam.
[00029] The biocidal composition may be added to steam make-up water for steam treatment to disinfect surfaces, and particularly large surface areas. Without restriction to a theory, the composition augments the disinfecting or cleaning action of the steam, particularly after condensation, where it is believed that the composition remains effective. Thus, the composition will be dispersed over the area which was steam treated and provide some additional and continuing biocidal effect. Examples of steam application include enclosed areas, piping, tanks and other vessels, or tank or vessel headspaces.
[00030] The biocidal composition may be added to a product as a preservative to prevent microbial growth. Such products may include foods, beverages, consumer products including personal care items such as soaps, shampoos, cosmetics, medications, solutions, industrial products or any product which requires or could benefit from a preservative additive.
[00031] In addition to its anti-bacterial properties, the inventors have demonstrated that the biocidal composition may have antiviral, anti-fungal or anti-algal properties.
[00032] The biocidal composition may also be effective to kill or limit the growth of fungi or fungi-like organisms. Fungi are eukaryotic organisms including yeasts and molds.
Fungi-like organisms may include Pythium or Phytophthora species. In particular, the biocidal composition may be effective to control fungal diseases which affect agricultural operations, such as clubroot (Plasmodiophora brassicae), and other fungi which cause wilting, mold, rust, blotches, scabs or decaying tissue on plants. Exemplary fungal diseases may include dollar spot of turfgrass, powdery mildew on ornamental plants, damping-off and root rot of beans, botrytis gray mold on greenhouse floral crops, scab of apple and crabapple, and Dutch elm disease.
Fungi-like organisms may include Pythium or Phytophthora species. In particular, the biocidal composition may be effective to control fungal diseases which affect agricultural operations, such as clubroot (Plasmodiophora brassicae), and other fungi which cause wilting, mold, rust, blotches, scabs or decaying tissue on plants. Exemplary fungal diseases may include dollar spot of turfgrass, powdery mildew on ornamental plants, damping-off and root rot of beans, botrytis gray mold on greenhouse floral crops, scab of apple and crabapple, and Dutch elm disease.
[00033] The biocidal composition may be used in horticultural or agricultural applications, where it may be directly applied to plants, seeds or seedlings, may be incorporated into a soil or other growth medium, or may be used to treat soil or other growth medium.
[00034] In one specific application, the biocide may be effective in limiting or controlling clubroot infections. Soil or plant seeds, or both, which may harbor clubroot fungal spores may be treated with the biocide, or a dilute solution of the biocide, to kill or inactivate the fungal spores.
[00035] The biocidal composition may also be effective to kill or limit the growth of algae. As an algaecide, it may be used in lakes, ponds, pools or freshwater or saltwater aquariums to control or eliminate algae.
[00036] The biocidal composition may be considered to be effective to kill a microorganism if it kills greater than 90.0%, 95.0%, 99.0%, or preferably greater than 99.9% of the starting inoculum. The biocidal composition may be considered to be effective to control or limit the growth of a microorganism if no visible growth occurs in a growth medium under conditions where visible growth does occur in a control sample.
[00037] The biocidal or growth inhibiting effect may be determined by various tests known to those skilled in the art. For example, bactericidal or growth inhibiting effect of a specific concentration of the composition may be determined using a minimal inhibitory concentration (MIC) test or similar compendial test, which determines if the composition at a given concentration inhibits visible bacterial growth after sufficient incubation time to see bacterial growth in a control sample. Alternatively, a minimal bactericidal concentration (MBC) may be determined by determining viability of organisms after exposure to the biocide. For example, the composition may be considered effective if it kills or renders inactive greater than 99.0%, and preferably greater than 99.9% of the starting inoculum after exposure to the composition.
[00038] A biocidal composition may be considered an effective preservative, for example in a foodstuff, drink, pharmaceutical or nutraceutical, if it passes standard tests, for example the preservative challenge tests specified in United States Pharmacopeia Chapter 51, European Pharmacopeia (Ph. Eur. or EP) 5.1.3, Japanese Pharmacopeia (JP) 19, or a similar compendia! test.
[00039] Exemplary embodiments of the present invention are described in the following Examples, which are set forth to aid in the understanding of the invention, and should not be construed to limit in any way the scope of the invention as defined in the claims which follow thereafter.
Example 1,¨ Preparation of electrolyzed water-amine composition
Example 1,¨ Preparation of electrolyzed water-amine composition
[00040] Electrolyzed water was prepared with either sodium chloride or potassium chloride at free chlorine concentrations of 100 to 8000 ppm. In one example, NaCI at about 6 g/litre or KC1 at about 7.6 g/litre was used to produce the electrolyzed water.
[00041] The biocide used in Examples below was prepared by mixing measured volumes of electrolyzed water (4,400 ppm ¨ 8000 ppm free available chlorine) made by combining equal volumes of anolyte and catholyte, with liquid monoethanolamine ((MEA), with less than 1% diethanolamine (DEA)), with stirring. The mixture heated up upon mixing, and was allowed to cool to room temperature before use. .
[00042] In one example, Formula A was produced by mixing 60% electrolyzed water and 40%
monoethanolamine (v:v) and Formula B was produced by mixing 15% electrolyzed water, 10%
monoethanolamine and 75% water (v:v). Both Formula A and B were found to have no detectable chlorine and a pH of about 13.1 and about 11.6 respectively.
Example 2 ¨ Clubroot Fungus Control
monoethanolamine (v:v) and Formula B was produced by mixing 15% electrolyzed water, 10%
monoethanolamine and 75% water (v:v). Both Formula A and B were found to have no detectable chlorine and a pH of about 13.1 and about 11.6 respectively.
Example 2 ¨ Clubroot Fungus Control
[00043] 2.5g of galled root material was ground in a blender with 50m1 distilled water. The spore suspension was filtered through 8 layers of cheesecloth and the number of resting spores was determined to be 3X108 spores/ml by counting on a haemocytometer.
[00044] Two products, Formula A and Formula B ability to kill clubroot spores was tested at differing concentrations both at room temperature and with the products heated to 80 C in a water bath.
[00045] I
ml of spore suspension was transferred to 1.5m1 tubes, the tubes were centrifuged and the liquid removed leaving a pellet of spores. The spore pellet was re-suspended in 1ml of treatment and left to incubate at room temperature for 20 minutes. The tubes were centrifuged and the treatment removed, the spore pellet was washed 3 times with I ml distilled water and finally re-suspended in I ml distilled water.
ml of spore suspension was transferred to 1.5m1 tubes, the tubes were centrifuged and the liquid removed leaving a pellet of spores. The spore pellet was re-suspended in 1ml of treatment and left to incubate at room temperature for 20 minutes. The tubes were centrifuged and the treatment removed, the spore pellet was washed 3 times with I ml distilled water and finally re-suspended in I ml distilled water.
[00046] 50u1 of the spore suspension was removed and mixed with an equal volume of Evans blue stain and left at room temperature for 24hrs. The stained sample was diluted 1:4 with 5% glycerol and aliquots were observed under 400X magnification. Resting spores for each treatment were observed and the results recorded, spores that appeared blue were recorded as dead while spores that had no blue colour were recorded as viable. The total number of dead and viable spores were counted in the viewing field of the microscope at three separate locations on the slide and the % mortality rate was calculated.
[00047] Resting spore mortality was unaffected in the negative control samples (distilled water) and was determined to be 100% in the positive control treatment of 2% sodium hypochlorite. Formula A
was very effective at 100% concentration at room temperature with a mortality rate of 90.6%. The efficacy of the product declined at the lower concentrations tested. When Formula A was heated to 80 C it was found to have a greater efficacy at 100% concentration with a mortality rate of 98%. The efficacy of Formula A heated to 80 C remained high even at the lower concentrations tested with a 90%
mortality rate being observed at when the product was diluted 1 in 25.
was very effective at 100% concentration at room temperature with a mortality rate of 90.6%. The efficacy of the product declined at the lower concentrations tested. When Formula A was heated to 80 C it was found to have a greater efficacy at 100% concentration with a mortality rate of 98%. The efficacy of Formula A heated to 80 C remained high even at the lower concentrations tested with a 90%
mortality rate being observed at when the product was diluted 1 in 25.
[00048] Formula B at 100% concentration at room temperature was observed to have an 85.5%
mortality rate. The efficacy of the product declined at the lower concentrations tested. When Formula B was heated to 80 C it was found to have a greater efficacy at the 100%
concentration with a mortality rate of 96.4%. The efficacy of Formula B heated to 80 C was higher at all concentrations tested compared to the product at room temperature.
mortality rate. The efficacy of the product declined at the lower concentrations tested. When Formula B was heated to 80 C it was found to have a greater efficacy at the 100%
concentration with a mortality rate of 96.4%. The efficacy of Formula B heated to 80 C was higher at all concentrations tested compared to the product at room temperature.
[00049]
Importantly, all seeds tested germinated in soils saturated with Formula A or B. With seeds placed in soils saturated with 2% sodium hypochlorite, no seeds germinated.
Importantly, all seeds tested germinated in soils saturated with Formula A or B. With seeds placed in soils saturated with 2% sodium hypochlorite, no seeds germinated.
[00050] In a separate test, a disinfectant formula (AES-100) was prepared using KC1 electrolyzed water (about 8000 ppm of free available chlorine, which was mixed 60:40 with MEA. Clubroot galls were collected from canola roots. These galls were then dried and ground in a laboratory mill with a coarse screen. The ground galls were then mixed in distilled water and filtered through cheese cloth to make a spore suspension of 2.2 x 108 spores mL-1) and transferred to 5 mL
tubes. These tubes were then centrifuged and the water was decanted leaving a pellet of spores. To these spore pellets 1 mL of the disinfectant was added, the spores re-suspended, and incubated at room temperature for 20 min.
Subsequently, 9 mL of universal neutralizer was added to neutralize the disinfectant and then sample was centrifuged again. The spore pellet was then rinsed with sodium phosphate buffer 3 times, and then re-suspended in 5 mL of sodium phosphate buffer. Aliquots of 50 tit of spore suspension were mixed with an equal volume of Evans blue stain and left for 24 hrs at room temperature. This stained suspension was then observed under a compound microscope at 1000X
magnification. One hundred resting spores were evaluated for each sample and the results recorded.
Resting spores that took up the stain and appeared blue inside were scored as "dead". Treatment samples were compared with negative controls and a standard treatment (bleach) and these values were used to determine the efficacy of each disinfectant treatment.
tubes. These tubes were then centrifuged and the water was decanted leaving a pellet of spores. To these spore pellets 1 mL of the disinfectant was added, the spores re-suspended, and incubated at room temperature for 20 min.
Subsequently, 9 mL of universal neutralizer was added to neutralize the disinfectant and then sample was centrifuged again. The spore pellet was then rinsed with sodium phosphate buffer 3 times, and then re-suspended in 5 mL of sodium phosphate buffer. Aliquots of 50 tit of spore suspension were mixed with an equal volume of Evans blue stain and left for 24 hrs at room temperature. This stained suspension was then observed under a compound microscope at 1000X
magnification. One hundred resting spores were evaluated for each sample and the results recorded.
Resting spores that took up the stain and appeared blue inside were scored as "dead". Treatment samples were compared with negative controls and a standard treatment (bleach) and these values were used to determine the efficacy of each disinfectant treatment.
[00051] Resting spore mortality was unaffected in the negative control (water) treatment. Sodium hypochlorite was used as a positive standard. For Na0C1, all concentrations above 1.5% resulted in 100% mortality of clubroot resting spores. The disinfectant formula was very effective at full strength or 100% concentration with 91.2% spore mortality. All concentrations above 20%
resulted in greater than 70% spore mortality.
Example 3 ¨ Anti-bacterial Effectiveness
resulted in greater than 70% spore mortality.
Example 3 ¨ Anti-bacterial Effectiveness
[00052] Bacterial test strains (Pseudomonas aeruginosa (ATCC 15442), Staphylococcus aureus (ATCC 6538) and Streptococcus pneumoniae (ATCC 49619)) were grown overnight and a bacterial cell suspension for each strain prepared. The suspension was adjusted to 1.5 x 108 cells per ml using McFarland Latex Turbidity Standards. After adjusting the cell concentration, 0.1 ml of the cell suspension was spread uniformly on trypticase soy agar (TSA) plates using spreaders. Reference may be had to Boyan Bonev, James Hooper, Judicael Parisot; Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method, Journal of Antimicrobial Chemotherapy, Volume 61, Issue 6, 1 June 2008, Pages 1295-1301, the entire contents of which are incorporated herein by reference.
[00053] Formula A was applied to the inoculated TSA plates using a paper disc method. Sterilized filter paper discs were saturated with 0.1 ml of the biocide and then placed on the surface of the inoculated TSA plates. The TSA plates were then incubated at 36aC for 36 hours. The biocide was also applied to the inoculated TSA plates using a well diffusion method. Using a cork borer size 9 mm, holes were punched on another set of the inoculated TSA agar plates. The holes were then filled with the biocide. The plates were then incubated (upright) at 36 C for 36 hours.
[00054] In each case (Figure 2), there were clear inhibition zones on the plates treated with the biocide, indicating bacteria being inhibited in the diffusion zone of the biocide.
[00055] In another test, effectiveness of the biocide as a preservative was determined. The biocide passed USP requirements for antimicrobial preservative effectiveness not only against bacteria, but also yeast (Candida) and a fungus (Aspergillus).
E. call 8739) 4.66 x 10 s <1 ci (ATCC
S.aureus (ATCC 6538) 8.28 x 10 5 <1 <1 P.aeruginosa 6.95 x 10 5 <1 <1 (ATCC 9027) C. albicans (ATCC 10231) 6.08 x 10 <1 <1 A. brasiliensis 415x 10 6 <1 <1 (ATCC 16404) Example 4 ¨ Anti-Viral Effectiveness
E. call 8739) 4.66 x 10 s <1 ci (ATCC
S.aureus (ATCC 6538) 8.28 x 10 5 <1 <1 P.aeruginosa 6.95 x 10 5 <1 <1 (ATCC 9027) C. albicans (ATCC 10231) 6.08 x 10 <1 <1 A. brasiliensis 415x 10 6 <1 <1 (ATCC 16404) Example 4 ¨ Anti-Viral Effectiveness
[00056] Formula A was diluted 1:1000 and applied to poliovirus. After 10 minutes, 89% of the viruses were rendered inactive.
Replicate (Glass Average Average pfu/mL Recovered From Carrier Treated Contact Time Percent Logio reduction Each Carrier' with Spray) Reduction 1 1.1 x 104 88.8% 0.95 2 1.2 x 104 87.8% 0.92 Minutes 3 1.2 x 104 87.8% 0.92 Control to x los (Unsprayed Gass NA NA
Carrier) 9.8 x 104
Replicate (Glass Average Average pfu/mL Recovered From Carrier Treated Contact Time Percent Logio reduction Each Carrier' with Spray) Reduction 1 1.1 x 104 88.8% 0.95 2 1.2 x 104 87.8% 0.92 Minutes 3 1.2 x 104 87.8% 0.92 Control to x los (Unsprayed Gass NA NA
Carrier) 9.8 x 104
[00057]
[00058] Each sterile glass carrier was inoculated with 10 microliters of diluted poliovirus stock and allowed to dry. Inoculated carriers were then sprayed to saturation (10 seconds) with the diluted Formula A. The solution was allowed a contact time of 10 minutes. The carriers were eluted in 10m1 of DIE Neutralizing Broth w/ Tween. Samples were enumerated for plaque forming units as per EPA
methodology (EPA625/R-92/013). Briefly, the eluates were assayed for the presence of infectious virus particles using plaque assay. The samples were analyzed in duplicates of 0.1 and 1.0 mL at various II
dilutions as per standard lab operating procedures. The positive controls were diluted 1/100 in phosphate buffered water (Weber Scientific) prior to analysis.
methodology (EPA625/R-92/013). Briefly, the eluates were assayed for the presence of infectious virus particles using plaque assay. The samples were analyzed in duplicates of 0.1 and 1.0 mL at various II
dilutions as per standard lab operating procedures. The positive controls were diluted 1/100 in phosphate buffered water (Weber Scientific) prior to analysis.
[00059] The control number represents the number of plaque forming units recovered from a control, non-treated slides that were inoculated, dried and tested as a positive control.
[00060] Definitions and Interpretation
[00061] The description of the present invention has been presented for purposes of illustration and description, but it is not intended to be exhaustive or limited to the invention in the form disclosed.
Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the invention. Embodiments were chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated. To the extent that the description is of a specific embodiment or a particular use of the invention, it is intended to be illustrative only, and not limiting of the claimed invention.
Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the invention. Embodiments were chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated. To the extent that the description is of a specific embodiment or a particular use of the invention, it is intended to be illustrative only, and not limiting of the claimed invention.
[00062] All terms and phrases used in this specification have their ordinary meanings as one of skill in the art would understand. Such ordinary meanings may be obtained by reference to technical dictionaries, such as Hawley's Condensed Chemical Dictionary 16th Edition, by R.J. Lewis, John Wiley & Sons, New York, N.Y., 2016.
[00063] References in the specification to "one embodiment", "an embodiment", etc., indicate that the embodiment described may include a particular aspect, feature, structure, or characteristic, but not every embodiment necessarily includes that aspect, feature, structure, or characteristic. Moreover, such phrases may, but do not necessarily, refer to the same embodiment referred to in other portions of the specification. Further, when a particular aspect, feature, structure, or characteristic is described in connection with an embodiment, it is within the knowledge of one skilled in the art to combine, affect or connect such aspect, feature, structure, or characteristic with other embodiments, whether or not such connection or combination is explicitly described. In other words, any element or feature may be combined with any other element or feature in different embodiments, unless there is an obvious or inherent incompatibility between the two, or it is specifically excluded.
[00064] It is further noted that the claims may be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for the use of exclusive terminology, such as "solely," "only," and the like, in connection with the recitation of claim elements or use of a "negative"
limitation. The terms "preferably," "preferred," "prefer," "optionally,"
"may," and similar terms are used to indicate that an item, condition or step being referred to is an optional (not required) feature of the invention.
limitation. The terms "preferably," "preferred," "prefer," "optionally,"
"may," and similar terms are used to indicate that an item, condition or step being referred to is an optional (not required) feature of the invention.
[00065] The singular forms "a," "an," and "the" include the plural reference unless the context clearly dictates otherwise. The term "and/or" means any one of the items, any combination of the items, or all of the items with which this term is associated.
[00066] As will be understood by one skilled in the art, for any and all purposes, particularly in terms of providing a written description, all ranges recited herein also encompass any and all possible sub-ranges and combinations of sub-ranges thereof, as well as the individual values making up the range, particularly integer values. A recited range (e.g., weight percents or carbon groups) includes each specific value, integer, decimal, or identity within the range. Any listed range can be easily recognized as sufficiently describing and enabling the same range being broken down into at least equal halves, thirds, quarters, fifths, or tenths. As a non-limiting example, each range discussed herein can be readily broken down into a lower third, middle third and upper third, etc.
[00067] As will also be understood by one skilled in the art, all language such as "up to", "at least", "greater than", "less than", "more than", "or more", and the like, include the number recited, and such terms refer to ranges that can be subsequently broken down into sub-ranges as discussed above. In the same manner, all ratios recited herein also include all sub-ratios falling within the broader ratio.
Claims (15)
1. A method of killing or controlling microorganisms excluding sulfate reducing bacterial species, comprising the step of contacting the microorganism with a composition comprising a reaction product of electrolyzed water and an amine.
2. The method of claim 1 wherein the microorganism comprises Gram negative bacteria, excluding Desulfovibrio species.
3. The method of claim 2 wherein the Gram negative bacteria comprises Salmonella, Shigella, Escherichia, Enterobacteriaceae, Pseudomonas, Moraxella, Helicobacter, Stenotrophomonas, Bdellovibrio, acetic acid bacteria, Legionella, cyanobacteria, spirochaetes, green sulfur, or green non-sulfur bacteria species.
4. The method of claim 1 wherein the microorganism comprises Gram positive bacteria.
5. The method of claim 4 wherein the microorganism comprises Bacillus, Corynebacteria, Clostridium, Listeria, Staphylococcus or Streptococcus species.
6. The method of claim I wherein the microorganism comprises a virus.
7. The method of claim 1 wherein the microorganism comprises a fungi.
8. The method of claim 7 wherein the fungi comprises a clubroot pathogen.
9. The method of claim 1 wherein the microorganism comprises an algae.
10. A method of disinfecting a surface, comprising the step of applying a biocidal composition comprising a reaction product of electrolyzed water and an amine to the surface.
11. The method of claim 10 wherein the biocidal composition is heated.
12. The method of claim 11 wherein the biocidal composition is mixed with water and heated to form steam, which is applied to the surface.
13. A method of treating a volume of water to kill or control a microorganism comprising the step of adding a biocidal composition comprising a reaction product of electrolyzed water and an amine to the volume of water.
14. A method of controlling a plant disease caused by a fungus, comprising contacting plant seeds, soil or other growth medium with a biocidal composition comprising a reaction product of electrolyzed water and an amine.
15. A method of preserving a product susceptible to microbial spoilage, comprising applying a biocidal composition comprising a reaction product of electrolyzed water and an amine to the product.
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CA2987632A CA2987632A1 (en) | 2017-12-04 | 2017-12-04 | Biocidal composition comprising electrolyzed water and an amine |
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CA2987632A CA2987632A1 (en) | 2017-12-04 | 2017-12-04 | Biocidal composition comprising electrolyzed water and an amine |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111995008A (en) * | 2020-08-21 | 2020-11-27 | 山西转型综改示范区晋中开发区维斯汉医药科技有限公司 | Preparation method and application method of electrolyzed water for treating helicobacter pylori infection |
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2017
- 2017-12-04 CA CA2987632A patent/CA2987632A1/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111995008A (en) * | 2020-08-21 | 2020-11-27 | 山西转型综改示范区晋中开发区维斯汉医药科技有限公司 | Preparation method and application method of electrolyzed water for treating helicobacter pylori infection |
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