WO2022180265A3 - Methode de production d'une forme periplasmique de la proteine crm197 - Google Patents

Methode de production d'une forme periplasmique de la proteine crm197 Download PDF

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Publication number
WO2022180265A3
WO2022180265A3 PCT/EP2022/054914 EP2022054914W WO2022180265A3 WO 2022180265 A3 WO2022180265 A3 WO 2022180265A3 EP 2022054914 W EP2022054914 W EP 2022054914W WO 2022180265 A3 WO2022180265 A3 WO 2022180265A3
Authority
WO
WIPO (PCT)
Prior art keywords
producing
seq
periplasmic
protein crm197
periplasmic form
Prior art date
Application number
PCT/EP2022/054914
Other languages
English (en)
Other versions
WO2022180265A2 (fr
Inventor
Marc DAUKANDT
Nicolas Havelange
Stéphane HUBERTY
Philippe Ledent
Christian Rodriguez
Original Assignee
Xpress Biologics
Curavac Europe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xpress Biologics, Curavac Europe filed Critical Xpress Biologics
Priority to JP2023552214A priority Critical patent/JP2024507994A/ja
Priority to EP22710025.2A priority patent/EP4298224A2/fr
Priority to CN202280017425.6A priority patent/CN116964206A/zh
Publication of WO2022180265A2 publication Critical patent/WO2022180265A2/fr
Publication of WO2022180265A3 publication Critical patent/WO2022180265A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C12R2001/16—Corynebacterium diphtheriae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention se rapporte à une méthode de production d'une forme périplasmique de la SEQ ID NO :12, au vecteur d'expression codant pour la SEQ ID NO :12 et pour la séquence signal, SEQ ID NO :2, permettant le ciblage de SEQ ID NO :12 vers l'espace périplasmique, ainsi qu'à la souche transformée par le vecteur d'expression.
PCT/EP2022/054914 2021-02-26 2022-02-28 Methode de production d'une forme periplasmique de la proteine crm197 WO2022180265A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2023552214A JP2024507994A (ja) 2021-02-26 2022-02-28 タンパク質ｃｒｍ１９７のペリプラズム形態を産生する方法
EP22710025.2A EP4298224A2 (fr) 2021-02-26 2022-02-28 Methode de production d'une forme periplasmique de la proteine crm197
CN202280017425.6A CN116964206A (zh) 2021-02-26 2022-02-28 生产蛋白crm197的周质形式的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BEBE2021/5137 2021-02-26
BE20215137A BE1029145B1 (fr) 2021-02-26 2021-02-26 Methode de production d'une forme periplasmique de la proteine crm197

Publications (2)

Publication Number Publication Date
WO2022180265A2 WO2022180265A2 (fr) 2022-09-01
WO2022180265A3 true WO2022180265A3 (fr) 2022-10-20

Family

ID=74853484

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2022/054914 WO2022180265A2 (fr) 2021-02-26 2022-02-28 Methode de production d'une forme periplasmique de la proteine crm197

Country Status (5)

Country Link
EP (1) EP4298224A2 (fr)
JP (1) JP2024507994A (fr)
CN (1) CN116964206A (fr)
BE (1) BE1029145B1 (fr)
WO (1) WO2022180265A2 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010150230A1 (fr) * 2009-06-25 2010-12-29 Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi Expression bactérienne d'un gène artificiel pour produire crm197 et ses dérivés
WO2011126811A2 (fr) * 2010-03-30 2011-10-13 Pfenex Inc. Expression de protéines de toxines recombinantes en forte quantité
WO2015134402A1 (fr) * 2014-03-03 2015-09-11 Scarab Genomics, Llc Production accrue de crm197 recombinante chez e. coli
EP3444269A1 (fr) * 2017-08-17 2019-02-20 National Research Council of Canada Systèmes et méthodes pour la production de polypeptides de toxine diphterique

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0917647D0 (en) 2009-10-08 2009-11-25 Glaxosmithkline Biolog Sa Expression system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010150230A1 (fr) * 2009-06-25 2010-12-29 Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi Expression bactérienne d'un gène artificiel pour produire crm197 et ses dérivés
WO2011126811A2 (fr) * 2010-03-30 2011-10-13 Pfenex Inc. Expression de protéines de toxines recombinantes en forte quantité
WO2015134402A1 (fr) * 2014-03-03 2015-09-11 Scarab Genomics, Llc Production accrue de crm197 recombinante chez e. coli
EP3444269A1 (fr) * 2017-08-17 2019-02-20 National Research Council of Canada Systèmes et méthodes pour la production de polypeptides de toxine diphterique

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AH-REUM PARK ET AL: "Efficient recovery of recombinant CRM197 expressed as inclusion bodies in E.coli", PLOS ONE, vol. 13, no. 7, 18 July 2018 (2018-07-18), pages e0201060, XP055694392, DOI: 10.1371/journal.pone.0201060 *
JOHANNA HAUSJELL ET AL: "E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)", MICROBIAL CELL FACTORIES, vol. 17, no. 1, 30 October 2018 (2018-10-30), XP055754032, DOI: 10.1186/s12934-018-1016-6 *
NOLL STEPHAN ET AL: "Gezielte Optimierung von Escherichia coli BL21(DE3) : Heterologe Proteinexpression", vol. 19, no. 2, 1 March 2013 (2013-03-01), DE, pages 211 - 213, XP055854861, ISSN: 0947-0867, Retrieved from the Internet <URL:https://link.springer.com/content/pdf/10.1007/s12268-013-0292-2.pdf> DOI: 10.1007/s12268-013-0292-2 *
PHILIPPE GOFFIN ET AL: "High-yield production of recombinant CRM197, a non-toxic mutant of diphtheria toxin, in the periplasm of Escherichia coli", BIOTECHNOLOGY JOURNAL, vol. 12, no. 7, 1 July 2017 (2017-07-01), DE, pages 1700168, XP055576196, ISSN: 1860-6768, DOI: 10.1002/biot.201700168 *

Also Published As

Publication number Publication date
CN116964206A (zh) 2023-10-27
BE1029145B1 (fr) 2022-09-27
JP2024507994A (ja) 2024-02-21
BE1029145A1 (fr) 2022-09-19
WO2022180265A2 (fr) 2022-09-01
EP4298224A2 (fr) 2024-01-03

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