WO2022179073A1 - 一种解硫胺素芽孢杆菌及其在染料废水脱色中的应用 - Google Patents

一种解硫胺素芽孢杆菌及其在染料废水脱色中的应用 Download PDF

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WO2022179073A1
WO2022179073A1 PCT/CN2021/115686 CN2021115686W WO2022179073A1 WO 2022179073 A1 WO2022179073 A1 WO 2022179073A1 CN 2021115686 W CN2021115686 W CN 2021115686W WO 2022179073 A1 WO2022179073 A1 WO 2022179073A1
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bacillus
decolorization
dye
thiamine
wastewater
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PCT/CN2021/115686
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English (en)
French (fr)
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黄开龙
张徐祥
杨庆
叶林
任洪强
刘宁
朱继业
阮在高
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南京江岛环境科技研究院有限公司
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Publication of WO2022179073A1 publication Critical patent/WO2022179073A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/308—Dyes; Colorants; Fluorescent agents
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/30—Nature of the water, waste water, sewage or sludge to be treated from the textile industry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • the present invention relates to a thiamine-solvent bacillus, and also relates to the application of the thiamine-solvent bacillus in the decolorization of dye wastewater.
  • the printing and dyeing industry is a large user of industrial water and wastewater in China.
  • about 10% to 20% of the dyes will become waste dyes and discharged with the wastewater.
  • the discharge of printing and dyeing wastewater in China is about 300 ⁇ 4 million m 3 /d, accounting for about 35% of the total industrial wastewater discharge.
  • the transparency of the water body will decrease, which will seriously affect the appearance of the water body.
  • it will consume the dissolved oxygen in the water and seriously damage the water body ecology. balance, threatening aquatic life and human safety.
  • Printing and dyeing wastewater has the characteristics of large changes in water quality, high COD content, deep chromaticity, high alkalinity (generally pH 8 to 10), high biological toxicity, high ammonia nitrogen concentration and high water temperature.
  • the water temperature in the stage is generally 35 ⁇ 55 °C (the temperature fluctuation range is large).
  • the decolorization of printing and dyeing wastewater mainly includes physical, chemical and biological methods. Physical methods mainly include extraction methods, adsorption methods, membrane separation methods, etc.; chemical methods mainly include chemical oxidation methods, electrochemical methods, chemical coagulation methods, etc.; biological methods use microorganisms to degrade dyes to achieve decolorization.
  • the most suitable decolorization temperature range for microorganisms is 30-40 °C, but in practice, due to the large intermittent output of printing and dyeing wastewater, the influent of the biochemical system is usually as high as 35-55 °C, and the temperature fluctuates greatly.
  • Chen Guotao et al. reported a thermophilic bacterium YB322 (accession number: ERS2778618), which is very similar to Aneurinibacillus thermoaerophilus, at pH 6.5 and temperature 55°C in "Screening, Identification and Decolorization Performance of an Azo Dye Decolorizing Bacteria".
  • thermophilic bacteria YB322 has high temperature sensitivity, limited growth in alkaline environment, and unstable decolorization efficiency.
  • the present invention is aimed at the existence of microorganisms in the prior art when decolorizing dye wastewater: only specific dyes (azo dyes), low treatment concentration, high temperature sensitivity, and growth in an alkaline environment. Due to the problem of unstable decolorization efficiency due to restrictions and the like, a thiamine solution bacillus is provided, the thiamine solution bacillus has a large temperature range and can decolorize high-concentration dye wastewater in an alkaline environment , and can also be applied to high-efficiency decolorization of many different types of dyes.
  • the Bacillus thiamine solution described in the present invention is named Bacillus thiamine solution (Aneurinibacillus sp.) YR-2, and the classification name is Bacillus thiamine solution (Aneurinibacillus sp.), and the strain number is YR-2, has been deposited in the China Center for Type Culture Collection in Wuhan, China, with the deposit number CCTCC M2020604, and the deposit date is October 19, 2020.
  • the Aneurinibacillus sp. YR-2 strain of the present invention is derived from the activated sludge of the biological section of the landfill leachate sewage treatment system, and after separation and purification, grows in a dye medium under aerobic conditions at 35°C Good, the edges of the cells are irregular, the diameter is 2-6 mm, and the colonies are off-white, opaque, and are Gram-positive bacteria.
  • the technical problem to be solved by the present invention is to provide the application of the above-mentioned thiamine-solubilizing bacillus in the decolorization of dye wastewater.
  • the application is as follows: inoculating a ring of Aneurinibacillus sp. YR-2 in an expansion medium, aerobic culture at 35° C. for 24-48 hours, and obtaining the expansion culture of YR-2 after the culture. product, inoculate the expanded culture product in the printing and dyeing wastewater for treatment; according to the volume fraction, the inoculation amount of Aneurinibacillus sp. YR-2 in the printing and dyeing wastewater is 5-6%, and the amount of the dye in the printing and dyeing wastewater is 5-6%.
  • the mass concentration is not less than 100mg/L; the dyes in the printing and dyeing wastewater include a mixture of one or more of reactive brilliant red X-3B, reactive black 5, methyl orange or malachite green.
  • the temperature of the printing and dyeing wastewater is 35-60 °C, preferably 40 °C, and the pH value is 6.5-8.5, preferably 7.5. Under these conditions, it is conducive to Bacillus thiamine solution (Aneurinibacillus sp.) YR-2 Decolorization of various dyes.
  • the thiamine-solubilizing bacillus of the present invention can reach 90% of reactive brilliant red dye wastewater with an initial concentration of 100mg/L under a wide range of temperature span conditions and an alkaline environment. At the same time, it can be applied to the high-efficiency decolorization of various dyes, and the decolorization rate of other dyes in the wastewater has reached at least 80%; the invention has important significance for the biological decolorization of printing and dyeing wastewater.
  • Fig. 2 is the decolorization efficiency figure of Bacillus thiamine solubilizing bacillus (Aneurinibacillus sp.) YR-2 under different temperature conditions;
  • Figure 3 is the decolorization effect of Aneurinibacillus sp. YR-2 on different dyes of the present invention.
  • Bacillus thiamine solubilizer (Aneurinibacillus sp.) YR-2 strain of the present invention is obtained by screening from the biological section activated sludge of a certain landfill leachate treatment system, and the specific process is as follows:
  • solid medium includes the following quality components: every liter of water contains agar 20g, peptone 2g, Na 2 HPO 4 1.35g, KH 2 PO 4 1.8g, glucose 5.0g, MgSO 4 ⁇ 7H 2 O 0.2g, reactive brilliant red 100mg, the pH of the medium is 7.0 ⁇ 7.5), at 35°C Static culture for 1-3 days to obtain a single colony;
  • step (3) after picking the single colony in step (3) for streaking and purifying, streak preservation on the slant to obtain pure strain;
  • step (4) Inoculate the pure strain-Aneurinibacillus sp. YR-2 obtained in step (4) for 1 cycle and inoculate it in the expansion medium under aerobic culture at 35°C for 24-48h to obtain YR-2. Expansion product.
  • the expansion medium of pure strain is one of LB medium, beef extract peptone medium or dye medium.
  • the LB medium includes components of the following quality: each liter of water contains 5.0 g of yeast powder, 10 g of sodium chloride and 10 g of peptone, and the pH of the LB medium is 7.0-7.5.
  • the beef extract peptone medium includes the following components: each liter of water contains 3 g of beef, 10 g of peptone and 5 g of sodium chloride, and the pH of the beef extract peptone medium is 7.2 to 8.
  • the dye medium includes the following quality components: 2 g of peptone per liter of water, 1.35 g of Na 2 HPO 4 , 1.8 g of KH 2 PO 4 , 5.0 g of glucose, 0.2 g of MgSO 4 ⁇ 7H 2 O, 25 to 100 mg of reactive brilliant red, The pH of the medium is 6.5-7.5.
  • the strain YR-2 with the best degradation effect and the fastest growth rate obtained by screening in Example 1 was identified. After identification, the strain YR-2 was Aneurinibacillus sp.
  • Aneurinibacillus sp. YR-2 grows well in LB medium under aerobic conditions at 35°C, with irregular edges, 2-6mm in diameter, off-white colonies, opaque, gram positive bacteria.
  • strain YR-2 was submitted to GenBank for BLAST comparison. From the BLAST results, strain sequences with a base sequence similarity greater than 99.9% with YR-2 were selected for multiple serial alignment by Clustal W, and a phylogenetic tree was constructed using MAGE. (See Figure 1) to obtain YR-2 and Bacillus thiamine solubilizing Bacillus Aneurinibacillus sp.NCCP-1217, the accession number is LC065244.1, the relationship is the closest, therefore, the identification strain YR-2 is Bacillus thiamine solubilizing.
  • the present invention measures the decolorization efficiency of simulated dye wastewater by Aneurinibacillus sp. YR-2 under different temperature conditions, and the specific steps are as follows:
  • the expansion product of Bacillus thiamine solution (Aneurinibacillus sp.) YR-2 obtained in Example 1 was inoculated in a liquid simulated dye wastewater medium by volume 5%, five groups of parallel experiments were set, and five groups of parallel experiments were performed.
  • the temperature of the experiment was set to 30°C, 35°C, 40°C, 50°C, and 60°C, the initial pH value of the dye wastewater was 7.2, and the culture was static for 72 hours.
  • the decolorization efficiency of the strain YR-2 at different temperatures is shown in Figure 2. .
  • the simulated dye wastewater culture medium consists of the following raw materials: 2 g of peptone, 1.35 g of Na 2 HPO 4 , 1.8 g of KH 2 PO 4 , 5 g of glucose, 0.2 g of MgSO 4 ⁇ 7H 2 O and 100 mg of reactive brilliant red per liter of water.
  • Aneurinibacillus sp. YR-2 can achieve a decolorization rate of more than 90% for reactive brilliant red dye at 35-60 °C.
  • the expansion product of Bacillus thiamine solution (Aneurinibacillus sp.) YR-2 obtained in Example 1 was inoculated in a liquid simulated dye wastewater medium by volume 5%, the dye wastewater temperature was 40 ° C, and the initial pH value was 7.5, and cultured for 72h.
  • the decolorization effect of strain YR-2 on different dyes is shown in Figure 3.
  • the simulated dye wastewater culture medium is composed of the following raw materials: 2 g of peptone per liter of water, 1.35 g of Na 2 HPO 4 , 1.8 g of KH 2 PO 4 , 5 g of glucose, 0.2 g of MgSO 4 ⁇ 7H 2 O and 100 mg of mixed dye; the mixed dye consists of It is composed of active brilliant red X-3B, active black 5, methyl orange and malachite green mixed in any proportion.
  • Aneurinibacillus sp. YR-2 has different decolorization efficiencies for different types of dyes.
  • the decolorization rate of reactive black 5 dyes for 72h is 80.47%, and the decolorization rate of other dyes for 72h is 80.47%.
  • the decolorization rates were all above 95%, which indicated that Aneurinibacillus sp. YR-2 could be suitable for the decolorization of different dyes. From the observation of the decolorization efficiency of YR-2, the decolorization rate in the early stage (24h) was fast, and the decolorization rate in the later stage (48-72h) was slow.
  • Aneurinibacillus sp. YR-2 of the invention can realize high-efficiency decolorization of different dyes under a wide temperature span, and increases the adaptability and applicability of functional microorganisms to printing and dyeing wastewater.

Abstract

提供了一种解硫胺素芽孢杆菌及其在染料废水脱色中的应用。其特征在于：所述解硫胺素芽孢杆菌命名为解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2，于2020年10月19日保藏于中国典型培养物保藏中心，保藏编号为CCTCC M2020604。

Description

一种解硫胺素芽孢杆菌及其在染料废水脱色中的应用 技术领域
本发明涉及一种解硫胺素芽孢杆菌，还涉及上述解硫胺素芽孢杆菌在染料废水脱色中的应用。
背景技术
印染行业是我国工业用水大户和废水排放大户，在纺织印染过程中，约有10％～20％的染料会成为废染料随废水排出，据不完全统计，我国印染废水的排放量约为300～400万m 3/d，约占整个工业废水排放量的35％，印染废水排入水体后，会导致水体透明度下降，严重影响水体的外观，此外还会消耗水中的溶解氧，严重破坏水体生态平衡，威胁水生生物及人类安全。
印染废水具有水质水量变化大、COD含量高、色度深，碱度大(一般pH为8～10)、生物毒性大、氨氮浓度高以及水温偏高等特点，印染废水经冷却处理后进入生化处理阶段的水温一般为35～55℃(温度波动范围大)。目前对于印染废水的脱色主要包含物理法、化学法及生物法。物理法主要包含萃取法、吸附法、膜分离法等；化学法主要包含化学氧化法、电化学法、化学混凝法等；生物法是利用微生物降解染料实现脱色。因物理与化学处理方法大多存在着成本高、能耗大、效率低、难实施，且极易产生二次污染等问题，因此不适合大规模应用。相比之下，生物法成本低、效率高、操作简单、降解彻底，且不会产生二次污染，因而受到广泛关注。
通常微生物最适宜的脱色温度范围为30～40℃，但实际情况下，由于印染废水间歇性产量大，生化系统进水通常高达35～55℃，温度波动大。陈国涛等在《一株偶氮染料脱色菌的筛选、鉴定及脱色性能研究》中报道一株与Aneurinibacillus thermoaerophilus极为相似的嗜热杆菌YB322(登录号：ERS2778618)，在pH为6.5、温度为55℃下，48h内对初始浓度为30mg/L偶氮染料脱色率达到了92.87％，但温度在45℃或65℃时，脱色率显著降低至60％～70％；pH增至7.5时，脱色率降低至50％～60％。综上，已报道的嗜热杆菌YB322对温度敏感度高、碱性环境下生长受限、脱色效率不稳定。
发明内容
发明目的：本发明针对现有技术中微生物在对染料废水进行脱色处理时存在的：只能针对特定染料(偶氮染料)、处理浓度低、对温度敏感度高，且在碱性环境下生长受限等导致脱色效率不稳定的问题，提供一种解硫胺素芽孢杆菌，该解硫胺素芽孢杆菌适用的温度范围大且能够在偏碱性环境下对高浓度的染料废水进行脱色处理，同时还能够适用于多种不同类型染料的高效率脱色。
技术方案：本发明所述的解硫胺素芽孢杆菌，命名为解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2，分类命名为解硫胺素芽孢杆菌(Aneurinibacillus sp.)，菌株号为YR-2，现已保藏于位于中国武汉的中国典型培养物保藏中心，保藏编号为CCTCC M2020604，保藏日期为2020年10月19日。
本发明解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2菌株来源于垃圾渗滤液污水处理系统生物段活性污泥中，经分离纯化后，在染料培养基中于35℃好氧条件下生长良好，菌体边缘不规则，直径大小为2～6mm，米白色菌落，不透明，为革兰氏阳性菌。
本发明还要解决的技术问题是提供上述解硫胺素芽孢杆菌在染料废水脱色中的应用。
所述的应用为：将解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2接种一环于扩大培养基中，于35℃下好氧培养24～48h，培养后得到YR-2的扩培产物，将扩培产物接种于印染废水中进行处理；按体积分数算，印染废水中解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2的接种量为5～6％，印染废水中染料的质量浓度不低于100mg/L；印染废水中的染料包括活性艳红X-3B、活性黑5、甲基橙或孔雀石绿中的一种或多种的混合。
菌体应用时，印染废水的温度为35～60℃，优选为40℃，pH值为6.5～8.5，优选为7.5，此条件下有利于解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2对各种染料的脱色。
有益效果：在厌氧/缺氧条件下，本发明解硫胺素芽孢杆菌能够在大范围温度跨度条件以及偏碱性环境下，对初始浓度为100mg/L的活性艳红染料废水可达到90％以上的脱色率，同时能够适用于各种不同染料的高效脱色，对废水中的其它染料均达到了至少80％的脱色率；本发明对印染废水的生物脱色具有重要意义。
附图说明
图1为本发明解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2系统发育树；
图2为本发明解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2在不同温度条件下的脱色效率图；
图3为本发明解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2在对不同染料的脱色效果。
具体实施方式
以下结合附图和具体实施例对本发明的技术方案做进一步说明。
实施例1
本发明解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2菌株是从某垃圾渗滤液处理 系统生物段活性污泥中筛选获得，具体过程如下：
(1)取某垃圾渗滤液生物处理段活性污泥；
(2)将得到的污泥按体积比10％接种至100mL灭菌后的染料培养基(染料培养基中的染料浓度为25mg/L)中，在生化培养箱中于35℃静置培养，观察染料培养基中颜色变化；培养至染料培养基无色后，将一级培养液按体积比10％接种至新的染料培养基(染料培养基的染料浓度为50mg/L)中，继续于35℃静置培养，观察染料培养基中颜色变化；培养至染料培养基无色后，将二级培养液按体积比5％接种至100mL新的染料培养基中(染料培养基中染料浓度为100mg/L)，培养至染料培养基无色后，再将三级培养液按体积比5％接种至100mL新的染料初始浓度为100mg/L的染料培养基中，如此在染料初始浓度为100mg/L的染料培养基中连续接种驯化3次，完成驯化；
(3)取出步骤(2)中驯化后的污泥，经过梯度稀释后，涂布于固体含活性艳红X-3B的培养基中(固体培养基包括如下质量组分：每升水中含琼脂20g、蛋白胨2g，Na 2HPO 4 1.35g，KH 2PO 4 1.8g，葡萄糖5.0g，MgSO 4·7H 2O 0.2g，活性艳红100mg，培养基的pH为7.0～7.5)，35℃下静态培养1～3d，获得单菌落；
(4)挑取步骤(3)中的单菌落划线纯化后，于斜面上划线保存，得到纯菌株；
(5)将步骤(4)得到的纯菌株-解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2接种1环于扩大培养基中35℃下好氧培养24～48h，得到YR-2的扩培产物。
纯菌株的扩大培养基为LB培养基、牛肉膏蛋白胨培养基或染料培养基中的一种。
LB培养基包括如下质量的组分：每升水中含酵母粉5.0g、氯化钠10g和蛋白胨10g，LB培养基的pH为7.0～7.5。
牛肉膏蛋白胨培养基包括如下质量的组分：每升水中含牛肉3g、蛋白胨10g和氯化钠5g，牛肉膏蛋白胨培养基的pH为7.2～8。
染料培养基包括如下质量组分：每升水中含蛋白胨2g，Na 2HPO 4 1.35g，KH 2PO 4 1.8g，葡萄糖5.0g，MgSO 4·7H 2O 0.2g，活性艳红25～100mg，培养基的pH为6.5～7.5。
实施例2
将实施例1中筛选得到降解效果最佳、生长速率最快的菌株YR-2进行鉴定，经鉴定，菌株YR-2为解硫胺素芽孢杆菌(Aneurinibacillus sp.)。
解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2，在LB培养基中，35℃好氧条件生长良好，菌体边缘不规则，直径大小2～6mm，米白色菌落，不透明，革兰氏阳性菌。
通过PCR扩增和Sanger法测序，得到YR-2菌株16S rRNA全序列如下(SEQ ID NO.1)：
Figure PCTCN2021115686-appb-000001
将菌株YR-2测序结果提交GenBank进行BLAST比较，从BLAST结果中挑选与YR-2碱基序列相似度大于99.9％的菌株序列，通过Clustal W进行多重系列比对，并利用MAGE构建系统发育树(见图1)得到YR-2与解硫胺素芽孢杆菌Aneurinibacillus sp.NCCP-1217，登录号为LC065244.1，亲缘关系最近，因此，鉴定菌株YR-2为解硫胺素芽孢杆菌。
实施例3
本发明解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2在不同温度条件下对模拟染料废水的脱色效率进行测定，具体步骤如下：
将实施例1中得到的解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2的扩培产物按体积比5％接种于液体的模拟染料废水培养基中，设置五组平行实验，五组平行实验的温度分别设置为30℃、35℃、40℃、50℃、60℃，染料废水初始pH值为7.2，静置培养72h，菌株YR-2在不同温度下对染料的脱色效率见图2。
模拟染料废水培养基由以下原料组成：每升水中含蛋白胨2g，Na 2HPO 4 1.35g，KH 2PO 4 1.8g，葡萄糖5g，MgSO 4·7H 2O 0.2g以及活性艳红100mg。
如图2所示，解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2在35～60℃下对活性艳红染料均可达到90％以上的脱色率。
实施例4
本发明解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2对不同染料脱色效果分析，具体步骤如下：
将实施例1中得到的解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2的扩培产物按体积比5％接种于液体模拟染料废水培养基中，染料废水温度为40℃，初始pH值为7.5，静置培养72h。菌株YR-2对不同染料的脱色效果见图3。
模拟染料废水培养基由以下原料组成：每升水中含蛋白胨2g，Na 2HPO 4 1.35g，KH 2PO 4 1.8g，葡萄糖5g，MgSO 4·7H 2O 0.2g以及混合染料100mg；混合染料由任意比例混合的活性艳红X-3B、活性黑5、甲基橙和孔雀石绿组成。
如图3所示，解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2对不同种类的染料脱色效率有所不同，其对活性黑5染料72h的脱色率达80.47％，对其他染料72h的脱色率均达到95％以上，表明解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2能适用于不同染料的脱色处理。从YR-2脱色效率观察，其前期(24h)脱色速率快，后期(48～72h)脱色速率变缓。
本发明解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2能够实现大范围温度跨度下对不同染料的高效脱色，增加了功能微生物对印染废水的适应性以及适用性。

Claims (8)

  1. 一种解硫胺素芽孢杆菌，其特征在于：所述解硫胺素芽孢杆菌命名为解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2，于2020年10月19日保藏于中国典型培养物保藏中心，保藏编号为CCTCC M2020604。
  2. 权利要求1所述的解硫胺素芽孢杆菌在染料废水脱色中的应用。
  3. 根据权利要求2所述的解硫胺素芽孢杆菌在染料废水脱色中的应用，其特征在于：将解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2接种一环于扩大培养基中，于35℃下好氧培养24～48h，培养后得到YR-2的扩培产物，将扩培产物接种于印染废水中进行处理。
  4. 根据权利要求3所述的解硫胺素芽孢杆菌在染料废水脱色中的应用，其特征在于：印染废水中染料的质量浓度不低于100mg/L。
  5. 根据权利要求3所述的解硫胺素芽孢杆菌在染料废水脱色中的应用，其特征在于：按体积分数算，印染废水中解硫胺素芽孢杆菌(Aneurinibacillus sp.)YR-2的接种量为5～6％。
  6. 根据权利要求3所述的解硫胺素芽孢杆菌在染料废水脱色中的应用，其特征在于：印染废水的温度为35～60℃。
  7. 根据权利要求3所述的解硫胺素芽孢杆菌在染料废水脱色中的应用，其特征在于：印染废水的pH值为6.5～8.5。
  8. 根据权利要求3所述的解硫胺素芽孢杆菌在染料废水脱色中的应用，其特征在于：印染废水中的染料包括活性艳红X-3B、活性黑5、甲基橙或孔雀石绿中的一种或多种的混合。
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