WO2022174606A1 - Infant formula milk powder rich in structured lipid opl, and preparation method therefor - Google Patents

Infant formula milk powder rich in structured lipid opl, and preparation method therefor Download PDF

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WO2022174606A1
WO2022174606A1 PCT/CN2021/124718 CN2021124718W WO2022174606A1 WO 2022174606 A1 WO2022174606 A1 WO 2022174606A1 CN 2021124718 W CN2021124718 W CN 2021124718W WO 2022174606 A1 WO2022174606 A1 WO 2022174606A1
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opl
milk powder
infant formula
rich
formula milk
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PCT/CN2021/124718
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French (fr)
Chinese (zh)
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陈历俊
赵军英
乔为仓
刘斌
刘茜
刘璐
刘妍
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北京三元食品股份有限公司
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/16—Agglomerating or granulating milk powder; Making instant milk powder; Products obtained thereby
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/1522—Inorganic additives, e.g. minerals, trace elements; Chlorination or fluoridation of milk; Organic salts or complexes of metals other than natrium or kalium; Calcium enrichment of milk
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/1528—Fatty acids; Mono- or diglycerides; Petroleum jelly; Paraffine; Phospholipids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/156—Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/158—Milk preparations; Milk powder or milk powder preparations containing additives containing vitamins or antibiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/145—Gasseri
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/515—Animalis

Definitions

  • the application relates to the technical field of formula milk powder, in particular to an infant formula milk powder rich in structured lipid OPL and a preparation method thereof.
  • breast milk is the most ideal natural food for infants.
  • the rich nutrients in breast milk provide energy and nutritional needs for the growth and development of infants and young children.
  • infant formula has become the main breastmilk supplement or breastmilk substitute for their infants in the early stages of growth. Therefore, the development of infant formula milk powder is based on breast milk, and on the basis of cow milk, the composition and structure of each nutrient are adjusted to make it close to breast milk in terms of nutrition and function.
  • Fat is an important nutritional component in breast milk, accounting for about 3-5% of the total nutritional content, and more than 98% of breast milk fat is triglyceride.
  • the main structural type of triglyceride is sn-USU type, U represents unsaturated fatty acid, S represents saturated fatty acid.
  • Common triglycerides are 1,3-dioleic acid-2-palmitic acid triglyceride (hereinafter referred to as OPO) and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride (hereinafter referred to as OPL) .
  • OPO 1,3-dioleic acid-2-palmitic acid triglyceride
  • OPL 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride
  • Linoleic acid in OPL is an essential fatty acid that can lower blood cholesterol and prevent atherosclerosis. Lack of linoleic acid in babies can cause dry skin, thickening of scales, and stunted growth. In recent years, most of the infant formula milk powders composed of breast milk nutrition in China are mainly supplemented with OPO, but few products with the addition of structural lipid OPL are considered.
  • the application provides an infant formula milk powder rich in structured fat OPL
  • the infant formula milk powder is prepared by adding raw milk as the main raw material and by adding mixed oil rich in structured fat OPL and other ingredients
  • the mixed oil containing structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 54-58% and the content of palmitic acid at sn-2 position >80% and mixed vegetable oil; the structural lipid OPL in the infant formula milk powder
  • the mass fraction of triglycerides in the milk powder is 6-15%.
  • the structural lipid OPL described in this application can be prepared by conventional methods in the prior art. Using palm stearic acid as raw material, the enzymatic acid hydrolysis of palm stearic acid was carried out in a solvent-free system to produce OPL by a two-step method. In the solvent-free system, the two-step method formed the structural lipid OPL with the mass fraction of 54%-58% and the content of palmitic acid at the sn-2 position greater than 80%.
  • the two-step method is the prior art, including but not limited to "Research Progress of Enzymatic Synthesis of Sn-2 Palmitate Glycerides, Wei Wei et al., Engineering. 2020 Vol. 6 No. 4, 406-414", The preparation method described in the Chinese patent application with the application number 201811360521.2 and the Chinese patent application with the application number 201811373483.4.
  • the mixed lipid rich in structured lipid OPL is composed of 57% by mass fraction, and the content of palmitic acid at sn-2 position is 83.2% structured lipid OPL is formulated with mixed vegetable oils.
  • the mixed vegetable oil includes one or more of palm kernel oil, sunflower oil, soybean oil, and coconut oil.
  • the content of Sn-1, Sn-2 and Sn-3 palmitic acid can be detected by quantitative carbon magnetic resonance spectroscopy. , and finally obtain the mixed oil rich in structural lipid OPL.
  • the steps of detection and deployment are the prior art, including but not limited to "Research Progress of Enzymatic Synthesis of Sn-2 Palmitic Acid Glycerides, Wei Wei et al., Engineering. 2020 Vol. 6 No. 4, 406-414", The method provided in the Chinese patent application with the application number of 201811360521.2 and the Chinese patent application with the application number of 201811373483.4.
  • the other ingredients include ordinary whey protein powder, fats, lactose, oligosaccharides, minerals, vitamins and/or optional nutrients;
  • the optional nutrients include one or more of choline, inositol, taurine and carnitine.
  • the other ingredients include lactose, ordinary whey protein powder containing ⁇ -lactalbumin, galactooligosaccharides, fructooligosaccharides, polyfructose, Docosahexaenoic acid, arachidonic acid, taurine, minerals, vitamins, nucleotides, L-carnitine tartrate, casein phosphopeptides and lutein.
  • the content of other ingredients in every 100g of the infant formula milk powder is: lactose 46.2g, ordinary whey protein powder containing ⁇ -whey protein 3.7g , galactooligosaccharides 0.89g, fructooligosaccharides 0.1g, polyfructose 0.07g, docosahexaenoic acid 0.045g, arachidonic acid 0.065g, taurine 0.038g, minerals 1.32g, vitamins 0.079 g, nucleotides 0.025g, L-carnitine tartrate 0.007g, casein phosphopeptide 0.045g, lutein 0.00007g.
  • the added amount of the mixed oil rich in structured fat OPL and the ratio of solid to liquid of raw cow milk are 1-3:80 kg/L.
  • the addition amount of the mixed oil rich in structural fat OPL and the solid-liquid ratio of raw cow milk are 1:46.5kg/L; other ingredients include lactose, Ordinary whey protein powder with alpha-lactalbumin, galactooligosaccharides, fructooligosaccharides, polyfructose, docosahexaenoic acid, arachidonic acid, taurine, minerals, vitamins, nucleosides Acid, L-Carnitine Tartrate, Casein Phosphopeptide and Lutein.
  • the content of main macronutrients in every 100 g of the infant formula milk powder is shown in Table 1.
  • the present application also provides a method for preparing an infant formula milk powder rich in structured lipid OPL: a wet processing technique is adopted, raw cow milk is used as raw milk, pre-sterilized after cleaning the milk, and added to the pre-sterilized raw cow milk.
  • the mixed oil and other ingredients rich in OPL structural fat are sterilized after homogenization, and then concentrated and spray-dried.
  • pre-sterilization conditions 85 °C ⁇ 88 °C, 30s; homogeneous condition 15MPa; sterilization conditions: 93 °C ⁇ 95 °C, 15s; spray drying Conditions: Inlet air temperature 150°C ⁇ 160°C, outlet air temperature 85°C ⁇ 90°C.
  • This application uses raw cow milk as the main raw material, and carries out processing by adding mixed oil rich in structural lipid OPL, ordinary whey protein powder, fats, lactose, oligosaccharides, minerals, vitamins and some optional nutrients. After the ingredients are standardized, the prepared infant formula milk powder is rich in structural lipid OPL.
  • the structural lipid OPL in infant formula milk powder accounts for 6-15% of the mass fraction of triglycerides in milk powder, which can effectively promote the growth of intestinal probiotics in infants and young children, thereby reducing infant constipation and defecation difficulties, and improving the absorption of fat energy. as well as promoting bone growth and development in infants.
  • the infant formula milk powder provided by this application is rich in structural lipid OPL, which is more suitable for breast milk samples in China, and is more suitable for Chinese infants and young children compared to the milk powder products currently on the market that only add OPO. It also further promoted the process of breastfeeding infant formula milk powder.
  • Figure 1 is a graph of the experimental data of the in vitro growth of different probiotics cultured in media containing different concentrations of OPL.
  • Lactobacillus geranium and Bifidobacterium animalis were obtained from commercial purchases, the test numbers of Lactobacillus gestationis strains were F002, F003, F020 and F024 respectively, and the test numbers of Bifidobacterium animalis strains were F032; the OPL standard product was the mass fraction It is 54% to 58% OPL structural fat.
  • TAG 52:3 were composed of palmitic acid (C16:0), oleic acid (C18:1) and linoleic acid (C18:2), which were mostly OPL structural lipids.
  • the fatty acid composition of TAG 52:2 is palmitic acid (C16:0) and oleic acid (C18:1), so TAG 52:2 is mostly OPO structure glycerides; and the content of both in breast milk varies with the lactation stage. increases with the increase.
  • An infant milk powder rich in structured lipid OPL an infant formula milk powder rich in structured lipid OPL, characterized in that the infant formula milk powder uses raw cow milk as a main raw material, and is mixed by adding a mixture rich in structured lipid OPL. It is prepared by adjusting oil and other ingredients; the mixed oil rich in structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 57%, and the content of palmitic acid at the sn-2 position is 83.2% and mixed vegetable oil;
  • the mixed vegetable oil includes palm kernel oil, sunflower oil, soybean oil and coconut oil;
  • the structural lipid OPL in the infant formula milk powder accounts for 6-15% of the mass fraction of triglycerides in the milk powder;
  • the other ingredients in each 100g of the infant formula milk powder are: 3.7g of ordinary whey protein powder containing ⁇ -lactalbumin, 46.2g of lactose, 0.89g of galactooligosaccharide, 0.1g of fructooligosaccharide, and 0.07g of polyfructose.
  • the raw material of above-mentioned formula adopts wet processing technology to prepare milk powder: take raw cow milk as raw milk, carry out pre-sterilization after purifying milk, add 1kg of mixed oil and fat ingredients rich in structural fat OPL in 46.5L of pre-sterilized raw cow milk, and Add other ingredients according to the above formula content, sterilize after homogenization, and then concentrate and spray dry to obtain.
  • the main process parameters are: pre-sterilization (85°C-88°C, 30s); homogenization 15mPa; sterilization (93°C-95°C, 15s); spray drying (inlet air temperature 150°C-160°C, outlet air temperature 85°C- 90°C).
  • An infant milk powder rich in structured lipid OPL an infant formula milk powder rich in structured lipid OPL, characterized in that the infant formula milk powder uses raw cow milk as a main raw material, and is mixed by adding a mixture rich in structured lipid OPL. It is prepared by adjusting oil and other ingredients; the mixed oil rich in structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 57%, and the content of palmitic acid at the sn-2 position is 83.2% and mixed vegetable oil;
  • the mixed vegetable oil includes palm kernel oil, sunflower oil, soybean oil and coconut oil;
  • the structural lipid OPL in the infant formula milk powder accounts for 6-15% of the mass fraction of triglycerides in the milk powder;
  • the other ingredients in each 100g of the infant formula milk powder are: 3.7g of ordinary whey protein powder containing ⁇ -lactalbumin, 46.2g of lactose, 0.89g of galactooligosaccharide, 0.1g of fructooligosaccharide, and 0.07g of polyfructose.
  • the raw material of above-mentioned formula adopts wet processing technology to prepare milk powder: take raw cow milk as raw milk, carry out pre-sterilization after cleaning the milk, add 1kg to be rich in the mixed grease ingredients of structured fat OPL in 80L of pre-sterilized raw cow milk, and press
  • the above formula content is added with other ingredients, sterilized after homogenization, and then concentrated and spray-dried to obtain.
  • the main process parameters are: pre-sterilization (85°C-88°C, 30s); homogenization 15mPa; sterilization (93°C-95°C, 15s); spray drying (inlet air temperature 150°C-160°C, outlet air temperature 85°C- 90°C).
  • An infant milk powder rich in structured lipid OPL an infant formula milk powder rich in structured lipid OPL, characterized in that the infant formula milk powder uses raw cow milk as a main raw material, and is mixed by adding a mixture rich in structured lipid OPL. It is prepared by adjusting oil and other ingredients; the mixed oil rich in structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 57%, and the content of palmitic acid at the sn-2 position is 83.2% and mixed vegetable oil;
  • the mixed vegetable oil includes palm kernel oil, sunflower oil, soybean oil and coconut oil;
  • the structural lipid OPL in the infant formula milk powder accounts for 6-15% of the mass fraction of triglycerides in the milk powder;
  • the other ingredients in each 100g of the infant formula milk powder are: 3.7g of ordinary whey protein powder containing ⁇ -lactalbumin, 46.2g of lactose, 0.89g of galactooligosaccharide, 0.1g of fructooligosaccharide, and 0.07g of polyfructose.
  • the raw material of above-mentioned formula adopts wet processing technology to prepare milk powder: take raw milk as raw milk, carry out pre-sterilization after purifying milk, add the mixed oil and fat ingredient that 1kg is rich in structural fat OPL in 26.7L of pre-sterilized raw milk, and Add other ingredients according to the above formula content, sterilize after homogenization, and then concentrate and spray dry to obtain.
  • the main process parameters are: pre-sterilization (85°C-88°C, 30s); homogenization 15mPa; sterilization (93°C-95°C, 15s); spray drying (inlet air temperature 150°C-160°C, outlet air temperature 85°C- 90°C).
  • the experimental results are shown in Figure 1.
  • the medium with different concentrations of OPL standard substances can promote the proliferation of probiotics, especially Lactobacillus ferritis F002, L. Proliferation of Bacillus F020.
  • the OPL standard used in this experiment is OPL structural lipid with a mass fraction of 54% to 58%.
  • the concentration is 10% to 25%
  • the mass fraction of OPL structural lipid in the medium is about 6% to 15%, as shown in Figure 1 It can be seen that the structural lipid OPL at this concentration can effectively promote the growth of intestinal probiotics.
  • the present application provides an infant formula milk powder rich in structured fat OPL and a preparation method thereof, wherein the infant formula milk powder uses raw cow milk as the main raw material, and is adjusted by adding mixed oil rich in structured fat OPL and other ingredients. into; the mixed oil rich in structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 54-58% and the content of palmitic acid at the sn-2 position >80% and mixed vegetable oil; the infant formula milk powder
  • the medium structure lipid OPL accounts for 6-15% of the mass fraction of triglycerides in the milk powder.
  • the proportion of sn-2 palmitic acid in the structural lipid OPL in the formula milk powder of the present application is adjusted to be close to that of breast milk fat, thereby reducing infant constipation and difficulty in defecation, improving fat energy absorption and promoting infant bone growth and development .

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Abstract

An infant formula milk powder rich in structured lipid OPL, and a preparation method therefor. The infant formula milk powder rich in structured lipid OPL is prepared by means of taking raw cow milk as the main raw material, adding a mixed grease rich in structured lipid OPL and other ingredients and performing adjustment, wherein the mixed grease rich in structured lipid OPL is prepared by means of blending structured lipid OPL and mixed vegetable oil, the mass fraction of the structured lipid OPL being 54%-58% and the content of palmitic acid at position sn-2 being more than 80%, and the structured lipid OPL accounts for 6%-15% by mass of triglyceride in the milk powder. The infant formula milk powder is processed by means of a wet method, and is obtained by means of using raw cow milk as raw material milk, performing pre-sterilizing after purifying the milk, adding a mixed grease rich in structured lipid OPL and other ingredients into the pre-sterilized raw cow milk, sequentially performing homogenizing and sterilizing, and performing concentrating and spray drying.

Description

一种富含结构脂OPL的婴儿配方乳粉及其制备方法A kind of infant formula milk powder rich in structural lipid OPL and preparation method thereof
交叉引用cross reference
本申请要求在中国专利局提交的、申请号为202110202904.2、申请名称为“一种富含结构脂OPL的婴儿配方乳粉及其制备方法”的中国专利申请的优先权，该申请的全部内容通过引用结合在本申请中。This application claims the priority of the Chinese patent application with the application number of 202110202904.2 and the application title of "an infant formula milk powder rich in structured lipid OPL and its preparation method" filed in the Chinese Patent Office, the entire content of which is approved by Reference is incorporated in this application.
技术领域technical field
本申请涉及配方奶粉技术领域，具体涉及一种富含结构脂OPL的婴儿配方乳粉及其制备方法。The application relates to the technical field of formula milk powder, in particular to an infant formula milk powder rich in structured lipid OPL and a preparation method thereof.
背景技术Background technique
母乳是婴儿最理想的天然食物，母乳中丰富的营养物质为婴幼儿的生长发育提供了能量和营养需求。但对于一些由于自身或外在因素导致母乳不足或无法实施母乳喂养者，婴幼儿配方奶粉成为其婴儿生长初期主要的母乳补充剂或母乳替代品。因此，婴幼儿配方乳粉的开发均以母乳为标准，在牛乳的基础上通过调整各营养素的组成和结构使其在营养和功能上均接近于母乳。Breast milk is the most ideal natural food for infants. The rich nutrients in breast milk provide energy and nutritional needs for the growth and development of infants and young children. However, for some people who are insufficient or unable to breastfeed due to their own or external factors, infant formula has become the main breastmilk supplement or breastmilk substitute for their infants in the early stages of growth. Therefore, the development of infant formula milk powder is based on breast milk, and on the basis of cow milk, the composition and structure of each nutrient are adjusted to make it close to breast milk in terms of nutrition and function.
脂肪是母乳中重要的营养成分，占总营养含量的3-5％左右，母乳脂肪中98％以上是甘油三酯。甘油三酯的主要结构类型为sn-USU型，U代表不饱和脂肪酸，S代表饱和脂肪酸。常见的甘油三酯有1,3-二油酸-2-棕榈酸甘油三酯(以下简称OPO)及1-油酸-2-棕榈酸-3-亚油酸甘油三酯(以下简称OPL)。现有研究技术表明，母乳中除了常见的OPO，OPL含量也很高，甚至超过OPO。OPL中的亚油酸是一种人体必需脂肪酸，能降低血液胆固醇，具有预防动脉粥样硬化的作用。婴儿缺乏亚油酸，会产生皮肤干燥、鳞屑增厚、发育生长迟缓等现象。而近年来，中国母乳营养组成的婴幼儿配方奶粉多以添加OPO为主，却很少有考虑添加结构脂OPL的产品。Fat is an important nutritional component in breast milk, accounting for about 3-5% of the total nutritional content, and more than 98% of breast milk fat is triglyceride. The main structural type of triglyceride is sn-USU type, U represents unsaturated fatty acid, S represents saturated fatty acid. Common triglycerides are 1,3-dioleic acid-2-palmitic acid triglyceride (hereinafter referred to as OPO) and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride (hereinafter referred to as OPL) . The existing research technology shows that in addition to the common OPO, the content of OPL in breast milk is also very high, even exceeding OPO. Linoleic acid in OPL is an essential fatty acid that can lower blood cholesterol and prevent atherosclerosis. Lack of linoleic acid in babies can cause dry skin, thickening of scales, and stunted growth. In recent years, most of the infant formula milk powders composed of breast milk nutrition in China are mainly supplemented with OPO, but few products with the addition of structural lipid OPL are considered.
此外，脂肪酸在甘油三酯上的位置会影响其消化吸收。牛乳以及普通植物油的棕榈酸大多分布在甘油三酯的sn-1位和sn-3位，sn-1位和sn-3位的棕榈酸在体内经过胰脂酶水解后会得到游离的棕榈酸，容易与体内的钙镁离子形成高熔点的皂而排出体外，造成婴幼儿矿物质和能量的流失，这些高熔点的皂也会导致婴儿出现粪便硬结以及便秘问题。而母乳脂肪中约70％的棕榈酸位于甘油三酯的sn-2位，能以sn-2单甘酯的形式被吸收，因此母乳能够有效避免棕榈酸和钙质的流失。而现有技术中婴幼儿配方奶粉的甘油三酯 中sn-2位的棕榈酸的含量均低于母乳中的甘油三酯中sn-2位的棕榈酸。In addition, the location of fatty acids on triglycerides affects their digestion and absorption. Most of the palmitic acid in bovine milk and common vegetable oil is distributed in the sn-1 and sn-3 positions of triglycerides, and the palmitic acid at the sn-1 and sn-3 positions will be hydrolyzed by pancreatic lipase in vivo to obtain free palmitic acid. , It is easy to form high-melting soap with calcium and magnesium ions in the body and excrete it, causing the loss of minerals and energy in infants and young children. These high-melting soaps can also cause fecal induration and constipation problems in infants. About 70% of palmitic acid in breast milk fat is located at the sn-2 position of triglycerides and can be absorbed in the form of sn-2 monoglycerides, so breast milk can effectively avoid the loss of palmitic acid and calcium. And the content of palmitic acid at sn-2 position in the triglyceride of infant formula milk powder in the prior art is all lower than the palmitic acid at sn-2 position in the triglyceride in breast milk.
因此，需对现有的婴儿配方奶粉进一步优化。Therefore, the existing infant formula milk powder needs to be further optimized.
发明内容SUMMARY OF THE INVENTION
发明目的Purpose of invention
有鉴于此，有必要针对上述的问题，提供一种富含结构脂OPL的婴儿配方乳粉，调整配方奶粉中结构脂OPL中sn-2位棕榈酸占总棕榈酸的比例，使其与母乳脂肪接近，从而减少婴儿便秘和排便困难，完善脂肪能量的吸收以及促进婴儿骨骼生长发育。In view of this, it is necessary to address the above problems, provide an infant formula milk powder rich in structural lipid OPL, adjust the ratio of sn-2 palmitic acid in the structural lipid OPL in the formula milk powder to total palmitic acid, so that it is comparable to breast milk. Fat close, thereby reducing infant constipation and defecation difficulties, improving the absorption of fat energy and promoting infant bone growth and development.
解决方案solution
为实现上述目的，本申请采取以下的技术方案：To achieve the above purpose, the application adopts the following technical solutions:
本申请提供了一种富含结构脂OPL的婴儿配方乳粉，所述婴儿配方乳粉以生牛乳为主要原料、通过添加富含结构脂OPL的混合油脂以及其他配料调整而成；所述富含结构脂OPL的混合油脂由质量分数为54～58％，且sn-2位置的棕榈酸的含量>80％的结构脂OPL与混合植物油调配而成；所述婴儿配方乳粉中结构脂OPL占乳粉中甘油三酯的质量分数为6～15％。The application provides an infant formula milk powder rich in structured fat OPL, the infant formula milk powder is prepared by adding raw milk as the main raw material and by adding mixed oil rich in structured fat OPL and other ingredients; The mixed oil containing structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 54-58% and the content of palmitic acid at sn-2 position >80% and mixed vegetable oil; the structural lipid OPL in the infant formula milk powder The mass fraction of triglycerides in the milk powder is 6-15%.
本申请所述结构脂OPL可由现有技术常规方法制备。以棕榈硬脂酸为原料，采用两步法在无溶剂体系中进行了酶解酸解棕榈硬脂酸生产OPL。在无溶剂体系中，两步法形成了质量分数为54％-58％且在sn-2位置棕榈酸的含量大于80％的结构脂OPL。所述两步法为现有技术，包括但不限于如《酶法合成sn-2棕榈酸甘油酯的研究进展，韦伟等，Engineering.2020年第6卷第4期，406-414》、申请号为201811360521.2的中国申请专利、申请号为201811373483.4的中国申请专利中所述的制备方法。The structural lipid OPL described in this application can be prepared by conventional methods in the prior art. Using palm stearic acid as raw material, the enzymatic acid hydrolysis of palm stearic acid was carried out in a solvent-free system to produce OPL by a two-step method. In the solvent-free system, the two-step method formed the structural lipid OPL with the mass fraction of 54%-58% and the content of palmitic acid at the sn-2 position greater than 80%. The two-step method is the prior art, including but not limited to "Research Progress of Enzymatic Synthesis of Sn-2 Palmitate Glycerides, Wei Wei et al., Engineering. 2020 Vol. 6 No. 4, 406-414", The preparation method described in the Chinese patent application with the application number 201811360521.2 and the Chinese patent application with the application number 201811373483.4.
优选地，在上述富含结构脂OPL的婴儿配方乳粉中，所述富含结构脂OPL的混合油脂由质量分数为57％，且sn-2位置的棕榈酸的含量为83.2％的结构脂OPL与混合植物油调配而成。Preferably, in the above-mentioned infant formula milk powder rich in structured lipid OPL, the mixed lipid rich in structured lipid OPL is composed of 57% by mass fraction, and the content of palmitic acid at sn-2 position is 83.2% structured lipid OPL is formulated with mixed vegetable oils.
进一步地，在上述富含结构脂OPL的婴儿配方乳粉中，所述混合植物油包括棕榈仁油、葵花籽油、大豆油、椰子油中的一种或多种。Further, in the above-mentioned infant formula milk powder rich in structured lipid OPL, the mixed vegetable oil includes one or more of palm kernel oil, sunflower oil, soybean oil, and coconut oil.
结构脂OPL与混合植物油调配时可通过定量核磁共振碳谱方法检测Sn-1、Sn-2和Sn-3位棕榈酸的含量，通过不断的检测、调配结构脂OPL和混合植物油的含量、分析，最终获得富含结构脂OPL的混合油脂。检测和调配的步骤为现有技术，包括但不限于如《酶法合成sn-2棕榈酸甘油酯的研究进展，韦伟等，Engineering.2020年第6卷第4期，406-414》、申请号为201811360521.2的中国申请专利、申请号为201811373483.4的中国申请专利中所提供的方法。When formulating structural lipid OPL and mixed vegetable oil, the content of Sn-1, Sn-2 and Sn-3 palmitic acid can be detected by quantitative carbon magnetic resonance spectroscopy. , and finally obtain the mixed oil rich in structural lipid OPL. The steps of detection and deployment are the prior art, including but not limited to "Research Progress of Enzymatic Synthesis of Sn-2 Palmitic Acid Glycerides, Wei Wei et al., Engineering. 2020 Vol. 6 No. 4, 406-414", The method provided in the Chinese patent application with the application number of 201811360521.2 and the Chinese patent application with the application number of 201811373483.4.
进一步地，在上述富含结构脂OPL的婴儿配方乳粉中，所述其他配料包括普通乳清蛋白粉、脂肪类、乳糖、低聚糖类、矿物质、维生素和/或可选营养成分；所述可选营养成分包括胆碱、肌醇、牛磺酸和、肉碱中的一种或几种。Further, in the above-mentioned infant formula milk powder rich in structural lipid OPL, the other ingredients include ordinary whey protein powder, fats, lactose, oligosaccharides, minerals, vitamins and/or optional nutrients; The optional nutrients include one or more of choline, inositol, taurine and carnitine.
进一步地，在上述富含结构脂OPL的婴儿配方乳粉中，所述其他配料包括乳糖、含α-乳清蛋白的普通乳清蛋白粉、低聚半乳糖、低聚果糖、多聚果糖、二十二碳六烯酸、花生四烯酸、牛磺酸、矿物质、维生素、核苷酸、L-肉碱酒石酸盐、酪蛋白磷酸肽和叶黄素。Further, in the above-mentioned infant formula milk powder rich in structural lipid OPL, the other ingredients include lactose, ordinary whey protein powder containing α-lactalbumin, galactooligosaccharides, fructooligosaccharides, polyfructose, Docosahexaenoic acid, arachidonic acid, taurine, minerals, vitamins, nucleotides, L-carnitine tartrate, casein phosphopeptides and lutein.
优选地，在上述富含结构脂OPL的婴儿配方乳粉中，每100g所述婴儿配方乳粉中其他配料的含量为：乳糖46.2g，含α-乳清蛋白的普通乳清蛋白粉3.7g，低聚半乳糖0.89g，低聚果糖0.1g，多聚果糖0.07g，二十二碳六烯酸0.045g，花生四烯酸0.065g，牛磺酸0.038g，矿物质1.32g，维生素0.079g，核苷酸0.025g，L-肉碱酒石酸盐0.007g，酪蛋白磷酸肽0.045g，叶黄素0.00007g。Preferably, in the above-mentioned infant formula milk powder rich in structural lipid OPL, the content of other ingredients in every 100g of the infant formula milk powder is: lactose 46.2g, ordinary whey protein powder containing α-whey protein 3.7g , galactooligosaccharides 0.89g, fructooligosaccharides 0.1g, polyfructose 0.07g, docosahexaenoic acid 0.045g, arachidonic acid 0.065g, taurine 0.038g, minerals 1.32g, vitamins 0.079 g, nucleotides 0.025g, L-carnitine tartrate 0.007g, casein phosphopeptide 0.045g, lutein 0.00007g.
进一步地，在上述富含结构脂OPL的婴儿配方乳粉中，所述富含结构脂OPL的混合油脂的添加量与生牛乳的料液比为1～3:80kg/L。Further, in the above-mentioned infant formula milk powder rich in structured fat OPL, the added amount of the mixed oil rich in structured fat OPL and the ratio of solid to liquid of raw cow milk are 1-3:80 kg/L.
优选地，在上述富含结构脂OPL的婴儿配方乳粉中，所述富含结构脂OPL的混合油脂的添加量与生牛乳的料液比为1:46.5kg/L；其他配料包括乳糖、含α-乳清蛋白的普通乳清蛋白粉、低聚半乳糖、低聚果糖、多聚果糖、二十二碳六烯酸、花生四烯酸、牛磺酸、矿物质、维生素、核苷酸、L-肉碱酒石酸盐、酪蛋白磷酸肽和叶黄素。每100g所述婴儿配方乳粉中主要宏量营养成分含量如表1所示。Preferably, in the above-mentioned infant formula milk powder rich in structural fat OPL, the addition amount of the mixed oil rich in structural fat OPL and the solid-liquid ratio of raw cow milk are 1:46.5kg/L; other ingredients include lactose, Ordinary whey protein powder with alpha-lactalbumin, galactooligosaccharides, fructooligosaccharides, polyfructose, docosahexaenoic acid, arachidonic acid, taurine, minerals, vitamins, nucleosides Acid, L-Carnitine Tartrate, Casein Phosphopeptide and Lutein. The content of main macronutrients in every 100 g of the infant formula milk powder is shown in Table 1.
表1 富含结构脂OPL的婴儿配方乳粉中主要宏量营养成分含量表Table 1 Contents of main macronutrients in infant formula milk powder rich in structured lipid OPL
Figure PCTCN2021124718-appb-000001
Figure PCTCN2021124718-appb-000001
本申请还提供了一种富含结构脂OPL的婴儿配方乳粉的制备方法：采用湿法加工工艺，以生牛乳作为原料乳，净乳后进行预杀菌，向经过预杀菌的生牛乳中添加富含OPL结构脂的混合油脂及其它配料，均质后进行杀菌，然后经浓缩、喷雾干燥，即得。The present application also provides a method for preparing an infant formula milk powder rich in structured lipid OPL: a wet processing technique is adopted, raw cow milk is used as raw milk, pre-sterilized after cleaning the milk, and added to the pre-sterilized raw cow milk. The mixed oil and other ingredients rich in OPL structural fat are sterilized after homogenization, and then concentrated and spray-dried.
进一步地，在上述富含结构脂OPL的婴儿配方乳粉的制备方法中，预杀菌条件：85 ℃～88℃，30s；均质条件15MPa；杀菌条件：93℃～95℃，15s；喷雾干燥条件：进风温度150℃～160℃，出风温度85℃～90℃。Further, in the above-mentioned preparation method of infant formula milk powder rich in structural lipid OPL, pre-sterilization conditions: 85 ℃～88 ℃, 30s; homogeneous condition 15MPa; sterilization conditions: 93 ℃～95 ℃, 15s; spray drying Conditions: Inlet air temperature 150℃～160℃, outlet air temperature 85℃～90℃.
本申请的有益效果为：The beneficial effects of this application are:
(1)本申请以生牛乳为主要原料，通过添加富含结构脂OPL的混合油脂、普通乳清蛋白粉、脂肪类、乳糖、低聚糖类、矿物质、维生素及一些可选营养成分进行配料标准化后，调制出的婴幼儿配方乳粉富含结构脂OPL。婴儿配方乳粉中结构脂OPL占乳粉中甘油三酯的质量分数为6～15％，可以有效促进婴幼儿肠道益生菌的生长，从而减少婴儿便秘和排便困难问题，完善脂肪能量的吸收以及促进婴儿骨骼生长发育。(1) This application uses raw cow milk as the main raw material, and carries out processing by adding mixed oil rich in structural lipid OPL, ordinary whey protein powder, fats, lactose, oligosaccharides, minerals, vitamins and some optional nutrients. After the ingredients are standardized, the prepared infant formula milk powder is rich in structural lipid OPL. The structural lipid OPL in infant formula milk powder accounts for 6-15% of the mass fraction of triglycerides in milk powder, which can effectively promote the growth of intestinal probiotics in infants and young children, thereby reducing infant constipation and defecation difficulties, and improving the absorption of fat energy. as well as promoting bone growth and development in infants.
(2)本申请提供的婴幼儿配方乳粉富含结构脂OPL，更加贴合中国地区的母乳样本，相对于目前市场上仅以添加OPO为主的奶粉产品，更加适用于中国婴幼儿使用，也进一步的推进了婴幼儿配方奶粉母乳化进程。(2) The infant formula milk powder provided by this application is rich in structural lipid OPL, which is more suitable for breast milk samples in China, and is more suitable for Chinese infants and young children compared to the milk powder products currently on the market that only add OPO. It also further promoted the process of breastfeeding infant formula milk powder.
附图说明Description of drawings
图1是含有不同浓度OPL的培养基培养不同益生菌体外生长实验数据图。Figure 1 is a graph of the experimental data of the in vitro growth of different probiotics cultured in media containing different concentrations of OPL.
具体实施方式Detailed ways
为使本申请的目的、技术方案和优点更加清楚，下面将结合本申请实施例，对本申请的技术方案作进一步清楚、完整地描述。需要说明的是，所描述的实施例仅仅是本申请一部分实施例，而不是全部的实施例。基于本申请中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本申请保护的范围。In order to make the purpose, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be further clearly and completely described below with reference to the embodiments of the present application. It should be noted that the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.
本申请中，格式乳杆菌和动物双歧杆菌通过市售购买获得，格式乳杆菌菌株试验编号分别为F002、F003、F020和F024，动物双歧杆菌菌株试验编号为F032；OPL标品为质量分数为54％～58％的OPL结构脂。In this application, Lactobacillus geranium and Bifidobacterium animalis were obtained from commercial purchases, the test numbers of Lactobacillus gestationis strains were F002, F003, F020 and F024 respectively, and the test numbers of Bifidobacterium animalis strains were F032; the OPL standard product was the mass fraction It is 54% to 58% OPL structural fat.
需要说明的是，实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市售购买获得的常规产品。It should be noted that, if the specific conditions are not indicated in the examples, it is carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used without the manufacturer's indication are conventional products that can be purchased from the market.
前期数据采集：Preliminary data collection:
对中国地区的母乳样本进行检测，测试母乳中甘油三酯的含量和比例。本申请共检测183份母乳样本分别为初乳(样本量n＝61)、成熟乳(样本量n＝61)和过渡乳(样本量n＝61)。检测结果如表2和表3所示。结果表明，母乳甘油三酯中含量最高的为TAG 52:3和TAG 52:2，其脂质占比分别为14.31％和13.00％。经MS DIAL 3.20定性分析发现，TAG 52:3的脂肪酸构成为棕榈酸(C16:0)、油酸(C18:1)和亚油酸(C18:2)，即其多为OPL结构脂质。TAG 52:2的脂肪酸构成为棕榈酸(C16:0)、油酸(C18:1)，因此TAG 52:2 中多为OPO结构甘油酯；且二者在母乳中的含量均随着泌乳阶段的增加而增加。Breast milk samples from China were tested for the content and ratio of triglycerides in breast milk. A total of 183 breast milk samples were tested in this application, including colostrum (sample size n=61), mature milk (sample size n=61) and transitional milk (sample size n=61). The test results are shown in Tables 2 and 3. The results showed that the highest content of TAG 52:3 and TAG 52:2 in breast milk triglycerides were 14.31% and 13.00% of lipids, respectively. Qualitative analysis by MS DIAL 3.20 found that the fatty acids of TAG 52:3 were composed of palmitic acid (C16:0), oleic acid (C18:1) and linoleic acid (C18:2), which were mostly OPL structural lipids. The fatty acid composition of TAG 52:2 is palmitic acid (C16:0) and oleic acid (C18:1), so TAG 52:2 is mostly OPO structure glycerides; and the content of both in breast milk varies with the lactation stage. increases with the increase.
表2 母乳中甘油酯含量及占比Table 2 Content and proportion of glycerides in breast milk
Figure PCTCN2021124718-appb-000002
Figure PCTCN2021124718-appb-000002
表3 三个泌乳期甘油酯的含量差异Table 3 Content differences of glycerides in three lactation periods
Figure PCTCN2021124718-appb-000003
Figure PCTCN2021124718-appb-000003
实施例1Example 1
根据检测的中国母乳甘油酯组成的数据，并以此为参考，设计一种富含结构脂OPL的婴儿奶粉。According to the detected data of glyceride composition of breast milk in China, and taking this as a reference, an infant milk powder rich in structural lipid OPL was designed.
一种富含结构脂OPL的婴儿奶粉，一种富含结构脂OPL的婴儿配方乳粉，其特征在于，所述婴儿配方乳粉以生牛乳为主要原料、通过添加富含结构脂OPL的混合油脂以及其他配料调整而成；所述富含结构脂OPL的混合油脂由质量分数为57％，且sn-2位置的棕榈酸的含量为83.2％的结构脂OPL与混合植物油调配而成；所述混合植物油包括棕榈仁油、葵花籽油、大豆油和椰子油；所述婴儿配方乳粉中结构脂OPL占乳粉中甘油三酯的质量分数为6～15％；An infant milk powder rich in structured lipid OPL, an infant formula milk powder rich in structured lipid OPL, characterized in that the infant formula milk powder uses raw cow milk as a main raw material, and is mixed by adding a mixture rich in structured lipid OPL. It is prepared by adjusting oil and other ingredients; the mixed oil rich in structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 57%, and the content of palmitic acid at the sn-2 position is 83.2% and mixed vegetable oil; The mixed vegetable oil includes palm kernel oil, sunflower oil, soybean oil and coconut oil; the structural lipid OPL in the infant formula milk powder accounts for 6-15% of the mass fraction of triglycerides in the milk powder;
其中每100g所述婴儿配方乳粉中其他配料为：含α-乳清蛋白的普通乳清蛋白粉3.7g，乳糖46.2g，低聚半乳糖0.89g，低聚果糖0.1g，多聚果糖0.07g，二十二碳六烯酸0.045g，花生四烯酸0.065g，牛磺酸0.038g，矿物质1.32g，维生素0.079g，核苷酸0.025g，L-肉碱酒石酸盐0.007g，酪蛋白磷酸肽0.045g，叶黄素0.00007g。The other ingredients in each 100g of the infant formula milk powder are: 3.7g of ordinary whey protein powder containing α-lactalbumin, 46.2g of lactose, 0.89g of galactooligosaccharide, 0.1g of fructooligosaccharide, and 0.07g of polyfructose. g, Docosahexaenoic acid 0.045g, arachidonic acid 0.065g, taurine 0.038g, minerals 1.32g, vitamins 0.079g, nucleotides 0.025g, L-carnitine tartrate 0.007g, cheese Protein phosphopeptide 0.045g, lutein 0.00007g.
将上述配方的原料采用湿法加工工艺制备奶粉：以生牛乳作为原料乳，净乳后进行预杀菌，向经过预杀菌的生牛乳46.5L中添加1kg富含结构脂OPL的混合油脂配料，并按上述配方含量加入其它配料，均质后进行杀菌，然后经浓缩、喷雾干燥，即得。主要工艺参数为：预杀菌(85℃-88℃，30s)；均质15mPa；杀菌(93℃-95℃，15s)；喷雾干燥(进风温度150℃-160℃，出风温度85℃-90℃)。The raw material of above-mentioned formula adopts wet processing technology to prepare milk powder: take raw cow milk as raw milk, carry out pre-sterilization after purifying milk, add 1kg of mixed oil and fat ingredients rich in structural fat OPL in 46.5L of pre-sterilized raw cow milk, and Add other ingredients according to the above formula content, sterilize after homogenization, and then concentrate and spray dry to obtain. The main process parameters are: pre-sterilization (85℃-88℃, 30s); homogenization 15mPa; sterilization (93℃-95℃, 15s); spray drying (inlet air temperature 150℃-160℃, outlet air temperature 85℃- 90°C).
实施例2Example 2
根据检测的中国母乳甘油酯组成的数据，并以此为参考，设计一种富含结构脂OPL的婴儿奶粉。According to the detected data of glyceride composition of breast milk in China, and taking this as a reference, an infant milk powder rich in structural lipid OPL was designed.
一种富含结构脂OPL的婴儿奶粉，一种富含结构脂OPL的婴儿配方乳粉，其特征在于，所述婴儿配方乳粉以生牛乳为主要原料、通过添加富含结构脂OPL的混合油脂以及其他配料调整而成；所述富含结构脂OPL的混合油脂由质量分数为57％，且sn-2位置的棕榈酸的含量为83.2％的结构脂OPL与混合植物油调配而成；所述混合植物油包括棕榈仁油、葵花籽油、大豆油和椰子油；所述婴儿配方乳粉中结构脂OPL占乳粉中甘油三酯的质量分数为6～15％；An infant milk powder rich in structured lipid OPL, an infant formula milk powder rich in structured lipid OPL, characterized in that the infant formula milk powder uses raw cow milk as a main raw material, and is mixed by adding a mixture rich in structured lipid OPL. It is prepared by adjusting oil and other ingredients; the mixed oil rich in structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 57%, and the content of palmitic acid at the sn-2 position is 83.2% and mixed vegetable oil; The mixed vegetable oil includes palm kernel oil, sunflower oil, soybean oil and coconut oil; the structural lipid OPL in the infant formula milk powder accounts for 6-15% of the mass fraction of triglycerides in the milk powder;
其中每100g所述婴儿配方乳粉中其他配料为：含α-乳清蛋白的普通乳清蛋白粉3.7g，乳糖46.2g，低聚半乳糖0.89g，低聚果糖0.1g，多聚果糖0.07g，二十二碳六烯酸0.045g，花生四烯酸0.065g，牛磺酸0.038g，矿物质1.32g，维生素0.079g，核苷酸0.025g，L-肉碱酒石酸盐0.007g，酪蛋白磷酸肽0.045g，叶黄素0.00007g。The other ingredients in each 100g of the infant formula milk powder are: 3.7g of ordinary whey protein powder containing α-lactalbumin, 46.2g of lactose, 0.89g of galactooligosaccharide, 0.1g of fructooligosaccharide, and 0.07g of polyfructose. g, Docosahexaenoic acid 0.045g, arachidonic acid 0.065g, taurine 0.038g, minerals 1.32g, vitamins 0.079g, nucleotides 0.025g, L-carnitine tartrate 0.007g, cheese Protein phosphopeptide 0.045g, lutein 0.00007g.
将上述配方的原料采用湿法加工工艺制备奶粉：以生牛乳作为原料乳，净乳后进行预杀菌，向经过预杀菌的生牛乳80L中添加1kg富含结构脂OPL的混合油脂配料，并按上述配方含量加入其它配料，均质后进行杀菌，然后经浓缩、喷雾干燥，即得。主要工艺参数为：预杀菌(85℃-88℃，30s)；均质15mPa；杀菌(93℃-95℃，15s)；喷雾干燥(进风温度150℃-160℃，出风温度85℃-90℃)。The raw material of above-mentioned formula adopts wet processing technology to prepare milk powder: take raw cow milk as raw milk, carry out pre-sterilization after cleaning the milk, add 1kg to be rich in the mixed grease ingredients of structured fat OPL in 80L of pre-sterilized raw cow milk, and press The above formula content is added with other ingredients, sterilized after homogenization, and then concentrated and spray-dried to obtain. The main process parameters are: pre-sterilization (85℃-88℃, 30s); homogenization 15mPa; sterilization (93℃-95℃, 15s); spray drying (inlet air temperature 150℃-160℃, outlet air temperature 85℃- 90°C).
实施例3Example 3
根据检测的中国母乳甘油酯组成的数据，并以此为参考，设计一种富含结构脂OPL的婴儿奶粉。According to the detected data of glyceride composition of breast milk in China, and taking this as a reference, an infant milk powder rich in structural lipid OPL was designed.
一种富含结构脂OPL的婴儿奶粉，一种富含结构脂OPL的婴儿配方乳粉，其特征在于，所述婴儿配方乳粉以生牛乳为主要原料、通过添加富含结构脂OPL的混合油脂以及其他配料调整而成；所述富含结构脂OPL的混合油脂由质量分数为57％，且sn-2位置的棕榈酸的含量为83.2％的结构脂OPL与混合植物油调配而成；所述混合植物油包括棕榈仁油、葵花籽油、大豆油和椰子油；所述婴儿配方乳粉中结构脂OPL占乳粉中甘油三酯的质量分数为6～15％；An infant milk powder rich in structured lipid OPL, an infant formula milk powder rich in structured lipid OPL, characterized in that the infant formula milk powder uses raw cow milk as a main raw material, and is mixed by adding a mixture rich in structured lipid OPL. It is prepared by adjusting oil and other ingredients; the mixed oil rich in structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 57%, and the content of palmitic acid at the sn-2 position is 83.2% and mixed vegetable oil; The mixed vegetable oil includes palm kernel oil, sunflower oil, soybean oil and coconut oil; the structural lipid OPL in the infant formula milk powder accounts for 6-15% of the mass fraction of triglycerides in the milk powder;
其中每100g所述婴儿配方乳粉中其他配料为：含α-乳清蛋白的普通乳清蛋白粉3.7g，乳糖46.2g，低聚半乳糖0.89g，低聚果糖0.1g，多聚果糖0.07g，二十二碳六烯酸0.045g，花生四烯酸0.065g，牛磺酸0.038g，矿物质1.32g，维生素0.079g，核苷酸0.025g，L-肉碱酒石酸盐0.007g，酪蛋白磷酸肽0.045g，叶黄素0.00007g。The other ingredients in each 100g of the infant formula milk powder are: 3.7g of ordinary whey protein powder containing α-lactalbumin, 46.2g of lactose, 0.89g of galactooligosaccharide, 0.1g of fructooligosaccharide, and 0.07g of polyfructose. g, Docosahexaenoic acid 0.045g, arachidonic acid 0.065g, taurine 0.038g, minerals 1.32g, vitamins 0.079g, nucleotides 0.025g, L-carnitine tartrate 0.007g, cheese Protein phosphopeptide 0.045g, lutein 0.00007g.
将上述配方的原料采用湿法加工工艺制备奶粉：以生牛乳作为原料乳，净乳后进行预杀菌，向经过预杀菌的生牛乳26.7L中添加1kg富含结构脂OPL的混合油脂配料，并按上述配方含量加入其它配料，均质后进行杀菌，然后经浓缩、喷雾干燥，即得。主要工艺参数为：预杀菌(85℃-88℃，30s)；均质15mPa；杀菌(93℃-95℃，15s)；喷雾 干燥(进风温度150℃-160℃，出风温度85℃-90℃)。The raw material of above-mentioned formula adopts wet processing technology to prepare milk powder: take raw milk as raw milk, carry out pre-sterilization after purifying milk, add the mixed oil and fat ingredient that 1kg is rich in structural fat OPL in 26.7L of pre-sterilized raw milk, and Add other ingredients according to the above formula content, sterilize after homogenization, and then concentrate and spray dry to obtain. The main process parameters are: pre-sterilization (85℃-88℃, 30s); homogenization 15mPa; sterilization (93℃-95℃, 15s); spray drying (inlet air temperature 150℃-160℃, outlet air temperature 85℃- 90°C).
体外实验评价In vitro experimental evaluation
结构脂OPL对婴幼儿肠道益生菌的促进作用Promotion effect of structural lipid OPL on intestinal probiotics in infants and young children
分别活化4株格式乳杆菌和1株动物双歧杆菌三代，以2％的接种量接种于含有不同浓度(0％，10％，15％，20％，25％)的OPL标品的MRS液体培养基中，37℃厌氧培养24h后，用PBS进行稀释，取合适稀释梯度涂布于MRS固体培养基中，37℃厌氧培养48h后，进行平板计数，并计算其菌落总数，以CFU/mL为单位报告。Activated 4 strains of Lactobacillus spp. and 1 strain of Bifidobacterium animalis for three generations respectively, and inoculated with 2% inoculum in MRS liquid containing different concentrations (0%, 10%, 15%, 20%, 25%) of OPL standard In the medium, after anaerobic culture at 37°C for 24h, dilute with PBS, and apply appropriate dilution gradient to MRS solid medium, after anaerobic culture at 37°C for 48h, count the plate, and calculate the total number of colonies, as CFU Reported in units of /mL.
实验结果如图1所示，与不添加OPL的培养基相比，添加不同浓度OPL标品的培养基可以促进益生菌的增殖，尤其显著的促进格式乳杆菌F002、格式乳杆菌F003和格式乳杆菌F020的增殖。本实验采用的OPL标品为质量分数为54％～58％的OPL结构脂，添加浓度为10％～25％时，培养基OPL结构脂的质量分数约为6％～15％，由图1可知，该浓度下的结构脂OPL均可以有效促进肠道益生菌生长。The experimental results are shown in Figure 1. Compared with the medium without OPL, the medium with different concentrations of OPL standard substances can promote the proliferation of probiotics, especially Lactobacillus ferritis F002, L. Proliferation of Bacillus F020. The OPL standard used in this experiment is OPL structural lipid with a mass fraction of 54% to 58%. When the concentration is 10% to 25%, the mass fraction of OPL structural lipid in the medium is about 6% to 15%, as shown in Figure 1 It can be seen that the structural lipid OPL at this concentration can effectively promote the growth of intestinal probiotics.
以上所述实施例仅表达了本申请的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对本申请专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本申请构思的前提下，还可以做出若干变形和改进，这些都属于本申请的保护范围。因此，本申请专利的保护范围应以所附权利要求为准。The above-mentioned embodiments only represent several embodiments of the present application, and the descriptions thereof are relatively specific and detailed, but should not be construed as a limitation on the scope of the patent of the present application. It should be pointed out that for those skilled in the art, without departing from the concept of the present application, several modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the scope of protection of the patent of the present application shall be subject to the appended claims.
工业实用性Industrial Applicability
本申请提供一种富含结构脂OPL的婴儿配方乳粉及其制备方法，其中，所述婴儿配方乳粉以生牛乳为主要原料、通过添加富含结构脂OPL的混合油脂以及其他配料调整而成；所述富含结构脂OPL的混合油脂由质量分数为54～58％且sn-2位置的棕榈酸的含量>80％的结构脂OPL与混合植物油调配而成；所述婴儿配方乳粉中结构脂OPL占乳粉中甘油三酯的质量分数为6～15％。本申请的调整配方奶粉中结构脂OPL中sn-2位棕榈酸占总棕榈酸的比例，使其与母乳脂肪接近，从而减少婴儿便秘和排便困难，完善脂肪能量的吸收以及促进婴儿骨骼生长发育。The present application provides an infant formula milk powder rich in structured fat OPL and a preparation method thereof, wherein the infant formula milk powder uses raw cow milk as the main raw material, and is adjusted by adding mixed oil rich in structured fat OPL and other ingredients. into; the mixed oil rich in structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 54-58% and the content of palmitic acid at the sn-2 position >80% and mixed vegetable oil; the infant formula milk powder The medium structure lipid OPL accounts for 6-15% of the mass fraction of triglycerides in the milk powder. The proportion of sn-2 palmitic acid in the structural lipid OPL in the formula milk powder of the present application is adjusted to be close to that of breast milk fat, thereby reducing infant constipation and difficulty in defecation, improving fat energy absorption and promoting infant bone growth and development .

Claims (10)

  1. 一种富含结构脂OPL的婴儿配方乳粉，其特征在于，所述婴儿配方乳粉以生牛乳为主要原料、通过添加富含结构脂OPL的混合油脂以及其他配料调整而成；所述富含结构脂OPL的混合油脂由质量分数为54～58％且sn-2位置的棕榈酸的含量>80％的结构脂OPL与混合植物油调配而成；所述婴儿配方乳粉中结构脂OPL占乳粉中甘油三酯的质量分数为6～15％。An infant formula milk powder rich in structured fat OPL, characterized in that, the infant formula milk powder is prepared by using raw cow milk as a main raw material and adjusted by adding a mixed oil rich in structured fat OPL and other ingredients; The mixed oil containing structural lipid OPL is prepared from the structural lipid OPL with a mass fraction of 54-58% and the content of palmitic acid at the sn-2 position >80% and mixed vegetable oil; the structural lipid OPL in the infant formula milk powder accounts for The mass fraction of triglycerides in the milk powder is 6-15%.
  2. 根据权利要求1所述的富含结构脂OPL的婴儿配方乳粉，其特征在于，所述富含结构脂OPL的混合油脂由质量分数为57％且sn-2位置的棕榈酸的含量为83.2％的结构脂OPL与混合植物油调配而成。The infant formula milk powder rich in structural lipid OPL according to claim 1, wherein the mixed oil and fat rich in structural lipid OPL is 57% by mass fraction and the content of palmitic acid at sn-2 position is 83.2 % structured lipid OPL is formulated with mixed vegetable oils.
  3. 根据权利要求1或2所述的富含结构脂OPL的婴儿配方乳粉，所述混合植物油包括棕榈仁油、葵花籽油、大豆油、椰子油中的一种或多种。The structured fat OPL-enriched infant formula milk powder according to claim 1 or 2, wherein the mixed vegetable oil comprises one or more of palm kernel oil, sunflower oil, soybean oil, and coconut oil.
  4. 根据权利要求1所述的富含结构脂OPL的婴儿配方乳粉，其特征在于，所述其他配料包括普通乳清蛋白粉、脂肪类、乳糖、低聚糖类、矿物质、维生素和/或可选营养成分；所述可选营养成分包括胆碱、肌醇、牛磺酸和、肉碱中的一种或几种。The infant formula milk powder rich in structural lipid OPL according to claim 1, wherein the other ingredients include ordinary whey protein powder, fats, lactose, oligosaccharides, minerals, vitamins and/or Optional nutrients; the optional nutrients include one or more of choline, inositol, taurine, and carnitine.
  5. 根据权利要求1所述的富含结构脂OPL的婴儿配方乳粉，其特征在于，所述其他配料包括乳糖、含α-乳清蛋白的普通乳清蛋白粉、低聚半乳糖、低聚果糖、多聚果糖、二十二碳六烯酸、花生四烯酸、牛磺酸、矿物质、维生素、核苷酸、L-肉碱酒石酸盐、酪蛋白磷酸肽和叶黄素。The infant formula milk powder rich in structural lipid OPL according to claim 1, wherein the other ingredients include lactose, ordinary whey protein powder containing α-lactalbumin, galactooligosaccharides, fructooligosaccharides , polyfructose, docosahexaenoic acid, arachidonic acid, taurine, minerals, vitamins, nucleotides, L-carnitine tartrate, casein phosphopeptide and lutein.
  6. 根据权利要求5所述的富含结构脂OPL的婴儿配方乳粉，其特征在于，每100g所述婴儿配方乳粉中其他配料的含量为：乳糖46.2g，含α-乳清蛋白的普通乳清蛋白粉3.7g，低聚半乳糖0.89g，低聚果糖0.1g，多聚果糖0.07g，二十二碳六烯酸0.045g，花生四烯酸0.065g，牛磺酸0.038g，矿物质1.32g，维生素0.079g，核苷酸0.025g，L-肉碱酒石酸盐0.007g，酪蛋白磷酸肽0.045g，叶黄素0.00007g。The infant formula milk powder rich in structured lipid OPL according to claim 5, wherein the content of other ingredients in every 100g of the infant formula milk powder is: lactose 46.2g, ordinary milk containing alpha-lactalbumin Albumin powder 3.7g, galactooligosaccharide 0.89g, fructooligosaccharide 0.1g, polyfructose 0.07g, docosahexaenoic acid 0.045g, arachidonic acid 0.065g, taurine 0.038g, minerals 1.32g, vitamin 0.079g, nucleotide 0.025g, L-carnitine tartrate 0.007g, casein phosphopeptide 0.045g, lutein 0.00007g.
  7. 根据权利要求6所述的富含结构脂OPL的婴儿配方乳粉，其特征在于，所述富含结构脂OPL的混合油脂的添加量与生牛乳的料液比为1～3:80kg/L。The infant formula milk powder rich in structured fat OPL according to claim 6, wherein the added amount of the mixed oil rich in structured fat OPL and the solid-liquid ratio of raw cow milk are 1～3:80kg/L .
  8. 根据权利要求7所述的富含结构脂OPL的婴儿配方乳粉，其特征在于，所述富含结构脂OPL的混合油脂的添加量与生牛乳的料液比为1:46.5kg/L；每100g所述婴儿配方乳粉中主要宏量营养成分含量如下：蛋白质11.6g、脂肪27g、碳水化合物56.4g、OPL 10g； 其中2位棕榈酸占总棕榈酸含量的40％；每100g所述婴儿配方乳粉的能量为2162KJ。The infant formula milk powder rich in structured fat OPL according to claim 7, is characterized in that, the addition amount of the described mixed oil rich in structured fat OPL and the solid-liquid ratio of raw cow milk are 1:46.5kg/L; The content of the main macronutrients in each 100g of the infant formula milk powder is as follows: protein 11.6g, fat 27g, carbohydrate 56.4g, OPL 10g; wherein the 2-position palmitic acid accounts for 40% of the total palmitic acid content; The energy of infant formula milk powder is 2162KJ.
  9. 权利要求1～8任意一项所述婴儿配方乳粉的制备方法：其特征在于，采用湿法加工工艺，以生牛乳作为原料乳，净乳后进行预杀菌，向经过预杀菌的生牛乳中添加富含OPL结构脂的混合油脂及其它配料，均质后进行杀菌，然后经浓缩、喷雾干燥，即得。The preparation method of the infant formula milk powder according to any one of claims 1 to 8: it is characterized in that, a wet processing technique is adopted, raw cow milk is used as raw milk, pre-sterilized after cleaning the milk, and added to the pre-sterilized raw cow milk Add mixed oil rich in OPL structural fat and other ingredients, sterilize after homogenization, and then concentrate and spray dry to obtain.
  10. 根据权利要求9所述的制备方法，其特征在于，预杀菌条件：85℃～88℃，30s；均质条件15MPa；杀菌条件：93℃～95℃，15s；喷雾干燥条件：进风温度150℃～160℃，出风温度85℃～90℃。The preparation method according to claim 9, characterized in that, pre-sterilization conditions: 85℃～88℃, 30s; homogeneous condition 15MPa; sterilization conditions: 93℃～95℃, 15s; spray drying conditions: inlet air temperature 150 ℃ ℃～160℃, outlet temperature 85℃～90℃.
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