CN111700125A - Application of pomfret oil or its fraction in preparing human milk substitute lipid - Google Patents
Application of pomfret oil or its fraction in preparing human milk substitute lipid Download PDFInfo
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Abstract
The invention discloses application of pomfret oil or a fractionation product thereof in preparation of human milk substitute fat, and belongs to the technical field of food. The natural oil and fat rarely contain UPU type triglyceride, and a few natural oil and fat are rich in OPO, but the content of OPL is very low. The highest content of triglycerides in golden pomfret oil is OPL, and the second is OPO, which is similar to the triglyceride composition of Chinese mother butterfat. Selecting golden pomfret oil, freshwater white pomfret or its fractionated matters from pomfret oil as base material oil, and adding other natural oil or modified oil to obtain human milk substitute fat. The human milk substitute fat has high similarity with the human milk fat, solves the problem of low absorption rate of fatty acid and calcium of infant formula milk powder, improves the absorption and utilization of energy, improves the hardness of excrement, and reduces the occurrence of constipation and intestinal diseases of infants.
Description
Technical Field
The invention relates to an application of pomfret oil or a fractionation product thereof in preparation of human milk substitute fat, and belongs to the technical field of food.
Background
The breast milk is the best food for infants and can provide all nutrients required by the infants for growth and development. The lipid component in breast milk, called human milk fat, accounts for about 3% to 5%, provides 40% to 50% of energy and essential fatty acids for infants and young children, and promotes the absorption of fat-soluble vitamins. Human milk fat is a complex mixture of fats and oils, and the triglycerides in human milk fat are mostly present in the sn-UPU form, such as 1, 3-dioleoyl-2-palmitoyl triglyceride (OPO) and 1-oleic-2-palmitic-3-oleic triglyceride (OPL). The unique distribution of fatty acids on the triglyceride backbone affects digestion, absorption and metabolism in infants. Many studies have shown that feeding breast milk fat is beneficial for the absorption of fat and calcium by infants, while feeding most natural fats causes problems such as poor fat absorption and calcium loss in infants, and that feeding breast milk fat prevents constipation in infants, whereas ingestion of natural fat generally causes constipation in infants (Teichert and Akoh, Stearidonic acid acidic acid in the sn-2position by enzymationalization for use as human milk fat enterprises, Journal of agricultural and Food Chemistry 2011,59, 5692-5701).
With the development of society, the employment opportunities of women are increased, the unnatural fertility rate is increased, and the breast-feeding rate of puerperae is continuously reduced due to various reasons such as insufficient understanding and self condition of breast-feeding. Therefore, infant formula has become the best substitute food, and its lipid component is called human milk substitute fat (HMFS). According to a large amount of literature reports, the content of OPO in human milk fat is the highest in many countries and regions, but the OPL is found in many regions of China to be the triglyceride with the highest content in Chinese breast milk, and the OPO is the second highest. At present, human milk substitute fat in the market is developed based on the composition of foreign breast milk fat, the components of the breast milk fat of Chinese people are not concerned, and the addition of OPL is not noticed in infant formula milk powder, so that the difference between the human milk substitute fat in the Chinese market and the breast milk fat of Chinese people is large, and the growth and development of infants are not facilitated. The results of grandson analysis on the composition of the human milk substitute fat in 180 infant formula milk powder in the Chinese market and comparison and scoring with the composition of Chinese mother milk fat show that the total similarity coefficient (G) of the human milk substitute fat in 180 milk powder and the Chinese milk fat is less than 60 points, and the similarity of 82.78% of the human milk substitute fat in milk powder is distributed between 20 and 50 points (the composition, similarity evaluation and preparation research of the human milk substitute fat, doctor academic thesis of south Jiangnan university, 2018), which shows that the composition difference between the human milk substitute fat in the current Chinese market and the Chinese mother milk fat is huge, and the development and continuous optimization of the human milk substitute fat which is more similar to the Chinese milk fat are necessary
The natural oil is rarely rich in UPU type triglyceride, and the Basha fish oil and the Black fish oil only contain a certain content of OPO, and the content of OPL is still low, which is not consistent with the breast milk fat composition of Chinese people. When only the black fish oil or the Bassa fish oil is used for blending, the problem of insufficient OPL in the oil for the infant milk powder cannot be solved, and the similarity with breast milk fat is low. In addition, the synthesis of OPL by enzymatic methods is less studied and expensive. Therefore, a new oil rich in OPL is needed to be found and applied to preparing human milk substitute fat.
Disclosure of Invention
In order to solve the problems, the invention discovers that golden pomfret oil and freshwater white pomfret oil (Colossoma brachypomum), wherein the golden pomfret oil comprises Trachinotus ovatus oil (Trachinotus ovatus), Trachinotus blochii oil (Trachinotus blochii) and the like, is rich in OPL and OPO, has similar fatty acid composition and distribution with breast milk, is the closest to the breast milk fat composition of Chinese people, is a better choice for infant formula milk powder in China, and has application potential in the aspect of human milk substitute fat.
The invention aims to provide application of pomfret oil or a fraction thereof in preparing human milk substitute fat or milk products.
In one embodiment of the invention, the pomfret oil comprises one or both of golden pomfret oil and freshwater white pomfret oil.
In one embodiment of the invention, the golden pompano fish oil is trachinotus ovatus oil or trachinotus blosskii oil.
In one embodiment of the present invention, the pomfret oil extract is obtained by fractionation of pomfret oil by a dry fractionation method or a solvent fractionation method.
In one embodiment of the invention, said pomfret oil or fraction thereof is used as a supplemental ingredient for OPO and OPL in the preparation of human milk substitute lipids.
The invention also provides a human milk fat-replacing composition, which is prepared by taking pomfret oil or a fractionated substance thereof as base oil and adding other natural grease and/or modified grease.
In one embodiment of the invention, the natural oil comprises one or more of soybean oil, peanut oil, palm oil, rapeseed oil, coconut oil, and basha fish oil.
In one embodiment of the present invention, the modified fat or oil includes an OPO-structured fat and/or an OPL-structured fat synthesized by an enzymatic method.
In one embodiment of the present invention, the pomfret oil extract is obtained by fractionation of pomfret oil by a dry fractionation method or a solvent fractionation method.
In one embodiment of the invention, in the preparation method of the golden pompano oil isolate, an extraction solvent is acetone or n-hexane; the mass-to-volume ratio (w/v, g/mL) of the pomfret oil to the solvent is 1: (2-10); the extraction temperature is-10 to 0 ℃; the time is 10-20 h.
In one embodiment of the invention, the human milk fat-substitute composition comprises, by mass, 0-10% soybean oil, 0-10% peanut oil, 0-10% palm oil, 10% -20% coconut oil, 40% -50% sandfish oil, 30% -45% golden pompano oil, or a fraction thereof.
In one embodiment of the present invention, preferably, the ratio of soybean oil: peanut oil: palm oil: coconut oil: bassa fish oil: OPO structural lipid: the mass ratio of the golden pomfret oil fraction is (0.05-1): (1-2): (0.05-1): (10-20): (40-50): (30-40).
In one embodiment of the invention, the human milk fat-substitute composition comprises, by mass, 0-10% soybean oil, 0-10% peanut oil, 0-10% palm oil, 10-20% coconut oil, 15-25% sandfish oil, 20-30% OPL structured fat, 25-40% freshwater white pomfret oil, or a fractionation product thereof.
In one embodiment of the present invention, preferably, the ratio of soybean oil: peanut oil: palm oil: bassa fish oil: coconut oil: OPL structural lipid: the mass ratio of the freshwater white pomfret oil is (0.1-5): (0.1-5): (0.1-5): (10-20): (20-25): (25-30): (25-30).
It is a third object of the present invention to provide a food product comprising the above human milk fat substitute composition.
The invention has the beneficial effects that:
(1) the high content of OPL in the base oil selected by the invention is not found in other natural source grease, and the OPL is the triglyceride with the highest content in Chinese mother milk fat, which is different from the foreign breast milk fat. Experiments show that the contents of palmitic acid, sn-2 palmitic acid, OPO and OPL in golden pomfret oil are 23.52%, 37.25%, 15.84% and 25.67% respectively, the contents of palmitic acid, sn-2 palmitic acid, OPO and OPL in freshwater white pomfret oil are 25.26%, 36.96%, 26.39% and 19.38% respectively, and the two kinds of fish oil both contain high content of OPL and OPO, have composition close to that of breast milk fat of Chinese people, and are excellent base oil for preparing human milk substitute fat.
(2) The invention also provides the human milk substitute fat, the similarity of the human milk substitute fat to Chinese breast milk fat in terms of fatty acid composition is more than 90%, the similarity of the human milk substitute fat to Chinese breast milk fat in terms of sn-2 site fatty acid composition is more than 80%, the similarity of the human milk substitute fat to Chinese breast milk fat in terms of triglyceride composition is more than 70%, and the human milk substitute fat meets the requirements of Chinese babies better.
(3) Compared with the existing enzymatic method and chemical method for synthesizing related structural grease, the base oil for preparing the human milk substitute grease provided by the invention has the advantages of simpler process, higher safety and suitability for industrial production.
(4) The human milk substitute fat has high similarity with the human milk fat, solves the problem of low absorption rate of fatty acid and calcium of infant formula milk powder, improves the absorption and utilization of energy, improves the hardness of excrement, and reduces the occurrence of constipation and intestinal diseases of infants.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of better illustrating the invention and is not intended to limit the invention thereto.
1. Method for measuring palmitic acid content
Putting 50mg fish oil into a 10mL graduated tube, adding 2mL of 0.5mol/L potassium hydroxide-methanol solution, saponifying at 65 ℃ for 30min, cooling, adding 2mL of 25% volume fraction boron trifluoride-methanol solution, and carrying out water bath at 70 ℃ for 5 min; adding 2mL of n-hexane, oscillating for 3-4min to extract fatty acid methyl ester, adding 4mL of saturated NaCl solution, taking the upper layer solution, adding anhydrous sodium sulfate, oscillating (centrifuging at 10000rpm for 5min), sucking by a syringe, passing through a membrane, detecting by gas chromatography, and calculating the palmitic acid content.
2. Method for measuring content of sn-2-position palmitic acid
50mg of the sample are dissolved in 1.0mL of chromatographic grade n-hexane, 7mL of Tris-HCl buffer (pH 7.6), 1.75mL of cholate solution (0.05%), 0.7mL of calcium chloride solution (2.2%) are added, vortexed for 2min, 50mg of porcine pancreatic lipase is added, the reaction is carried out at 37 ℃ for 9min, and vortexed for 2min every 3 min. After the reaction is finished, 2mL of anhydrous ether is added into the cooled sample, the cooled sample is centrifuged at the rotating speed of 4000r/min, the organic upper layer is absorbed, nitrogen is concentrated to 200 mu L of sample, the sample is applied on a thin-layer chromatography, and a developing agent is n-hexane: anhydrous ether: glacial acetic acid (50:50:1, v/v/v), developing by using 2, 7-dichlorofluorescein, identifying under an ultraviolet lamp, scraping 2-MAG, extracting twice by using anhydrous ether, drying the anhydrous ether by using nitrogen, carrying out fatty acid methyl esterification on a sample, detecting and analyzing by using a gas chromatograph, and calculating the content of sn-2-palmitic acid.
3. Method for measuring content of triglyceride
The triglyceride composition of the analytical product was determined using a reverse phase high performance liquid chromatograph (RP-HPLC) equipped with an evaporative light detector. And (3) dissolving 20mg of sample in 1.0mL of chromatographic grade n-hexane, filtering by a membrane, and detecting and analyzing by a high performance liquid chromatograph. And (3) completing quantification by adopting a peak area normalization method, calibrating by using a standard substance to convert the area percentage into the mass percentage, and calculating the content of the triglyceride.
4. Similarity evaluation of human milk substitute lipids
The similarity of the human milk substitute fat related by the invention and Chinese breast milk fat in the aspects of the composition of fatty acid, sn-2 site fatty acid and triglyceride is evaluated by adopting a 'deduction' principle according to a human milk fat similarity evaluation model established by Xiaoxiaqiang and the like (Xiaoxiaqiang, human milk fat substitute evaluation model established by an enzyme method and prepared by the method [ D ]: Boshi academic paper, Wuxi: Jiannan university, 2013.). The human milk fat similarity evaluation model is as follows:
wherein G represents the total score of similarity evaluation, and the full score is 100 points; gFA/sn-2FA/TAGA similarity evaluation score representing a fatty acid, a sn-2 fatty acid or a triglyceride; n is the number of indices in the total fatty acids or sn-2 fatty acids or triglycerides; biActual values representing total fatty acids or sn-2 fatty acids or triglycerides of human milk substitute lipids; a isiThe terminal value of the total fatty acids or sn-2 fatty acids or triglycerides of human milk fat, depending on biThe size of (2).
Example 1: golden pomfret oil
Extracting golden pomfret oil by chloroform-methanol method, freeze drying fresh golden pomfret liver, and pulverizing. Taking 10g of the crushed powder, adding 100mL of methanol, shaking, adding 200mL of chloroform, carrying out ultrasonic treatment for 20min, carrying out suction filtration, taking clear liquid, adding 100mL of sodium chloride solution with the mass fraction of 0.88%, centrifuging the mixed solution at 4500rpm for 10min, sucking the lower layer, placing the lower layer in a round-bottom flask, and carrying out rotary evaporation to remove the solvent to obtain the golden pompano fish oil. The contents of palmitic acid, sn-2 palmitic acid, OPO and OPL in the golden pomfret oil were measured to be 23.52%, 37.25%, 15.84% and 25.67%, respectively.
Example 2: freshwater white pomfret oil
The method for extracting the fish oil of the freshwater white pomfret by the chloroform methanol method in the reference example 1. The contents of palmitic acid, sn-2 palmitic acid, OPO and OPL in the freshwater white pomfret oil are respectively 25.26%, 36.96%, 26.39% and 19.38%. The content of OPL is slightly lower than that of golden pomfret oil, and a small amount of OPL structural lipid can be added for preparing human milk substitute lipid.
Example 3: preparation method of fractionated product of golden pomfret oil
And (2) carrying out solvent fractionation on the golden pomfret oil obtained in the example 1, wherein the used solvent is acetone, the ratio (w/v) of the golden pomfret oil to the solvent is 1:8, the fractionation temperature is-6 ℃, and the fractionation time is 12h, so as to obtain a golden pomfret oil fraction. The contents of palmitic acid, sn-2-position palmitic acid, OPO and OPL in the golden pomfret oil fraction are respectively 27.82%, 45.37%, 20.28% and 29.46%.
Example 4: composition for replacing fat in human milk
Soybean oil, peanut oil, palm oil, coconut oil, Bassa fish oil and the fractionated product of the golden pompano fish oil in example 3 are selected as base oil, and the formula is programmed and solved through an Fmincon function in Matlab software to obtain the optimal proportion. The optimal mixture ratio is soybean oil: peanut oil: palm oil: coconut oil: bassa fish oil: 0.48 percent of golden pompano fish oil extract, 1.45 percent of golden pompano fish oil extract, 0.44 percent of golden pompano fish oil extract, 18.16 percent of golden pompano fish oil extract, 42.14 percent of golden pompano fish oil extract, 37.32 percent of golden pompano fish oil extract, wherein the total fatty acids, the sn-2position fatty acid and the triglyceride have the following similarity scores with the milk fat of Chinese mothers: 95.77, 84.84, and 77.42, with a total similarity score of 86.01. The score shows that the milk fat is similar to the milk fat of China. At present, the total similarity score of the human milk substitute fat in the formula milk powder in the Chinese market is below 60 points (composition, similarity evaluation and preparation research of Sunwong. human milk substitute fat, doctor academic thesis of Jiangnan university, 2018), so the invention obviously improves the similarity of the human milk substitute fat and the milk fat of Chinese mother.
Example 5: human milk fat-replacing composition
Selecting soybean oil, peanut oil, palm oil, coconut oil, Bassa fish oil, OPL structural grease and the freshwater white pomfret oil of example 2 as base oil, wherein the mixture ratio is soybean oil: peanut oil: palm oil: coconut oil: bassa fish oil: OPL structural lipid: 4.45 percent of freshwater white pomfret oil, 1.10 percent of freshwater white pomfret oil, 4.93 percent of freshwater white pomfret oil, 14.41 percent of freshwater white pomfret oil, 20.05 percent of freshwater white pomfret oil, 28.93 percent of freshwater white pomfret oil and 26.13 percent of freshwater white pomfret oil, wherein the total fatty acids, the sn-2position fatty acid and the triglyceride have the following similarity scores respectively: 97.52, 84.63, and 81.09, and a total similarity score of 87.74. The score shows that the milk fat is similar to the milk fat of China. At present, the total similarity score of the human milk substitute fat in the formula milk powder in the Chinese market is below 60 points (composition, similarity evaluation and preparation research of Sunwong. human milk substitute fat, doctor academic thesis of Jiangnan university, 2018), so the invention obviously improves the similarity of the human milk substitute fat and the milk fat of Chinese mother.
Example 6: infant formula milk powder
An infant formula comprising the human milk substitute lipid composition of example 4. The composition comprises (by weight portion): 400 parts of skim milk, 300 parts of desalted whey powder, 100 parts of human milk substitute fat composition, 5 parts of phospholipid, 2 parts of DHA powder, 2 parts of arachidonic acid powder, 50 parts of white granulated sugar, 50 parts of lactose, 20 parts of concentrated whey protein powder, 10 parts of galacto-oligosaccharide, 20 parts of fructo-oligosaccharide, 1 part of sialic acid, 1 part of nucleotide, 5 parts of choline, 0.2 part of lutein, 2 parts of compound vitamin and 2 parts of compound mineral substance.
Example 7: formula milk powder for old people
An elderly formula containing the human milk substitute lipid composition of example 5. The composition comprises (by weight portion): 500 parts of skim milk powder, 200 parts of whey protein powder, 50 parts of human milk substitute fat composition, 20 parts of marine fish bone collagen oligopeptide powder, 10 parts of galacto-oligosaccharide, 10 parts of fructo-oligosaccharide, 2 parts of compound vitamin and 2 parts of compound mineral.
Comparative example 1: grass carp oil
The method comprises extracting grass carp oil with chloroform methanol as reference in example 1, freeze drying fresh grass carp liver, and pulverizing. Taking 10g of the crushed powder, adding 100mL of methanol, shaking, adding 200mL of chloroform, performing ultrasonic treatment for 20min, performing suction filtration, taking clear liquid, adding 100mL of sodium chloride solution with the mass fraction of 0.88%, centrifuging the mixed solution at 4500rpm for 10min, sucking the lower layer, placing the lower layer in a round-bottom flask, and performing rotary evaporation to remove the solvent to obtain the grass carp oil. The contents of palmitic acid and sn-2 palmitic acid in grass carp oil were measured to be 15.76% and 21.52%, respectively, and calculated as sn-2 palmitic acid accounting for 45.51% of all palmitic acid contents, and OPO and OPL accounting for 10.27% and 3.14%, respectively. Has a large difference with the breast milk fat of Chinese people, and is not suitable to be used as base oil to prepare human milk substitute fat.
Comparative example 2: bassa fish oil
The basmati oil extracted by the chloroform methanol method according to example 1 was found to contain, in the basmati oil, palmitic acid at the sn-2position, OPO and OPL, 33.83%, 50.65%, 27.25% and 14.39%, respectively. Compared with the breast milk fat of Chinese people, the content of OPO is higher. OPO can be supplemented by adding Bassa oil, but the content of OPL is lower than that of human milk fat, so other oil containing OPL is required to be supplemented for blending.
Comparative example 3: silver carp oil
The chub oil extracted by the chloroform methanol method according to example 1 was found to have 5.22% and 12.33% of OPO and OPL, respectively. Its OPL and OPO content is much lower than that of human milk fat and is not suitable for use as a human milk substitute fat.
TABLE 1 triglyceride composition in different fish oils
Comparative example 4:
a human milk substitute lipid composition was prepared with reference to example 4, except that the golden pomfret oil fraction was omitted and the conditions were otherwise the same as in example 1. Soybean oil, peanut oil, palm oil, rapeseed oil, coconut oil and Bassa fish oil are selected as base oil, and the formula is programmed and solved through an Fmincon function in Matlab software to obtain the optimal proportion. The optimal mixture ratio is that soybean oil, peanut oil, palm oil, coconut oil, Bassa fish oil and OPO structural fat are 20.30 percent, 0.37 percent, 8.62 percent, 0.25 percent, 18.15 percent and 52.30 percent, the similarity scores of the three parts of sn-2position fatty acid and triglyceride and Chinese mother butterfat are respectively as follows: 95.56, 56.75 and 74.37, with a total similarity score of 75.68. Of these, the sn-2 fatty acids and triglycerides score more off than in the examples, since palmitic acid is distributed mainly in the sn-1,3 positions in these oils, which is detrimental to digestive absorption in infants. And the OPL content in various oils is low, the maximum is only 14.39%, and the OPO content is far higher than the OPL. Therefore, human milk substitute lipids with higher OPL than OPO content cannot be obtained by blending.
Comparative example 5: commercial infant formula milk powder
Weighing 1 g of commercial cow milk/vegetable oil-based infant formula milk powder, adding 10mL of 65 ℃ hot water, fully mixing and cooling. Adding 2.0mL of ammonia water, fully mixing, putting into a water bath kettle at about 65 ℃, heating for 15-20 min, taking out from time to time, and oscillating. After removal, the mixture was cooled to room temperature, 10mL of ethanol was added, and the mixture was gently but thoroughly mixed. Adding 25mL of diethyl ether and petroleum ether respectively for extraction, taking the upper organic phase after layering, and repeating for 2-3 times. All organic phases were combined, the organic solvent was removed by rotary evaporation at 40 ℃ and the resulting fat was stored in a freezer at-20 ℃ until use. The commercial infant formula was found to have a palmitic acid, oleic acid, linoleic acid content of 20.03%, 31.63% and 20.44% and a sn-2 palmitic acid content of 33.94% based on total fatty acids. The OPO content was 10.31%, and OPL was not detected. The total fatty acids, sn-2 fatty acids and triglycerides were evaluated separately and scored as 85.78, 44.89 and 39.72, with an average score of 56.80, with more losses in sn-2 fatty acids and triglycerides than the infant formula in example 3.
The compositions of fatty acid, sn-2 site fatty acid and triglyceride of the Chinese mother milk fat are shown in tables 2-3, and the data are derived from the composition, similarity evaluation and preparation research of Sunwong human milk substitute fat [ D ] [ Boshi academic thesis ]. Wuxi Jiangnan university, 2018.
Fatty acid and sn-2 fatty acid composition of milk fat of Chinese mother's milk in Table 2
Note: "-" indicates no concern.
TABLE 3 triglyceride composition of milk fat of Chinese mothers
Table 4 shows the fatty acid, sn-2 fatty acid and triglyceride compositions of golden pomfret oil in example 1 of the present invention.
TABLE 4 golden pomfret oil fatty acid, sn-2 fatty acid and triglyceride composition
Tables 5-7 compositions of fatty acids, sn-2 fatty acids and triglycerides of natural and modified oils involved in the physical blending process.
TABLE 5 Total fatty acid composition (%)
Note: ND means not detected.
TABLE 6 fatty acid composition (%) of sn-2 site of natural oils and modified oils
TABLE 7 triglyceride composition of natural oils and fats and modified oils (%)
Note: ND means not detected.
The lipid metabolism assay was performed on the human milk substitute lipids formulated in example 4, example 5 and comparative example 4 as follows:
laboratory animal cleaning grade Wistar rats (1 week old, male) were housed in the center of cleaning grade laboratory animals at a temperature (23+2 ℃), a humidity of 60%, free access to water and food. The care and pretreatment of the experimental animals are carried out according to the relevant regulations of the regulations on the management of experimental animals. After 28 male Wistar rats were prepared and fed with basal diet for one week, they were randomly divided into 4 groups, experimental group 1, experimental group 2, experimental group 3 and basal control group. After one week of adaptation, the animals are fed according to the feed formula. The feed formulations of the groups are prepared according to the feed formulation of experimental animals recommended by the American society for Nutrition, and the fat in the experimental group 1, the experimental group 2, the experimental group 3 and the basic control group are respectively human milk substitute fat and palm oil prepared in the example 4, the example 5 and the comparative example 4 with equal amount. The fat sources of all the feeds are different, and the energy, various main nutrients, mineral substances and various trace elements are consistent. Bedding and drinking water were changed daily, pups were fed individually in metabolic cages, feeding experiments were performed for 2 weeks, and average food intake and weight gain were recorded.
TABLE 8 basic diet formulation for young mice
The feeding experiment was carried out for two weeks, and the feces were collected for the last 3 days in time, respectively. At the end of each day, the collected feces were weighed and stored frozen at-20 ℃. After the experimental period is finished, all the manure samples in the cages are respectively mixed and uniformly mixed, a part of the mixture is frozen and dried, crushed and sieved by a 40-mesh sieve, and the mixture is frozen and stored at the temperature of minus 20 ℃. The lipid and mineral composition of the mouse feces was determined and the results are shown in tables 9 and 10.
TABLE 9 dietary intake and growth status in mice
During the two-week feeding experiment, there was no significant difference in dietary intake and fecal output between the groups, and the body weights of experiment group 1 and experiment group 2 had a certain tendency to increase compared to the other two groups. The color of feces was darker and the water content was lower in the experimental group 3 and the basal control group than in the other two groups.
TABLE 10 apparent absorption rate of fat and minerals in mice
Note: apparent absorption rate (intake-content in feces)/intake × 100%
The results of the lipid and mineral composition of mouse feces are shown in Table 10. Total fat refers to the sum of triacylglycerols and free fatty acids in feces, and experimental group 1 was not significantly different from experimental group 2, and had a lower fat excretion amount than experimental group 3 and the basal control group. It can be seen that the human milk in example 4 and example 5 replaces lipids to favor fat absorption.
The percentage of fecal calcium in experimental group 1 and experimental group 2 was significantly lower than that in experimental group 3 and the basal control group, and the apparent absorption rate of calcium was negatively correlated therewith. There was no significant difference in the apparent absorption of magnesium. This is probably because palmitic acid is easily combined with calcium at the sn-1,3 position to form calcium soap, which has low solubility in bile and is hardly absorbed by fat and calcium in the soap. It can be concluded that the human milk substitute lipids prepared in example 4 and example 5 reduce calcium excretion in feces and improve calcium absorption.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. Application of pomfret oil or its fraction in preparing human milk substitute fat or milk product is provided.
2. The use of claim 1, wherein the pomfret oil comprises one or both of golden pomfret oil and freshwater white pomfret oil.
3. The use of claim 2, wherein the golden pompano fish oil comprises trachinotus ovatus oil or trachinotus blosskii oil.
4. The use of claim 1, wherein the pomfret oil is obtained by fractionation of pomfret oil by dry fractionation or solvent fractionation.
5. The human milk fat-replacing composition is characterized by being prepared by taking pomfret oil or a fractionated substance thereof as base oil and adding other natural grease and/or modified grease.
6. The human milk fat substitute composition of claim 5, wherein the natural fat comprises one or more of soybean oil, peanut oil, palm oil, coconut oil, and basha fish oil.
7. The human milk substitute lipid composition of claim 5, wherein the modified lipid comprises an OPO structural lipid and/or an OPL structural lipid.
8. The human milk fat-substitute composition according to any one of claims 5 to 7, which comprises, in mass percent, 0 to 10% of soybean oil, 0 to 10% of peanut oil, 0 to 10% of palm oil, 10 to 20% of coconut oil, 40 to 50% of sandwort oil, and 30 to 45% of golden pompano oil or a fraction thereof.
9. The human milk fat-substitute composition according to any one of claims 5 to 7, which comprises, in mass percent, 0 to 10% of soybean oil, 0 to 10% of peanut oil, 0 to 10% of palm oil, 10 to 20% of coconut oil, 15% to 25% of basha fish oil, 20% to 30% of OPL structured fat, and 25% to 40% of freshwater white pomfret oil or a fraction thereof.
10. A food product comprising the human milk substitute lipid composition of any one of claims 5-9.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010545637.4A CN111700125A (en) | 2020-06-16 | 2020-06-16 | Application of pomfret oil or its fraction in preparing human milk substitute lipid |
| CN202011477848.5A CN112369472B (en) | 2020-06-16 | 2020-12-14 | Application of pomfret oil or its extract in preparation of breast milk substitute fat |
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| CN202011477848.5A Active CN112369472B (en) | 2020-06-16 | 2020-12-14 | Application of pomfret oil or its extract in preparation of breast milk substitute fat |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112369472A (en) * | 2020-06-16 | 2021-02-19 | 江南大学 | Application of pomfret oil or its extract in preparation of breast milk substitute fat |
| CN112522330A (en) * | 2020-11-27 | 2021-03-19 | 江南大学 | Medium-long carbon chain triglyceride for breast milk to replace fat and preparation method thereof |
| CN112841440A (en) * | 2021-02-02 | 2021-05-28 | 江南大学 | Pig milk fat-replacing composition based on triglyceride |
| WO2022174606A1 (en) * | 2021-02-22 | 2022-08-25 | 北京三元食品股份有限公司 | Infant formula milk powder rich in structured lipid opl, and preparation method therefor |
| CN115644262A (en) * | 2022-05-05 | 2023-01-31 | 上海交通大学 | Grease composition and application thereof |
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| CN113575685A (en) * | 2021-07-30 | 2021-11-02 | 江南大学 | Fish oil composition added to infant formula milk powder |
| CN115500390A (en) * | 2022-09-22 | 2022-12-23 | 黑龙江飞鹤乳业有限公司 | Digestible fatty acid ester composition |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105670781B (en) * | 2016-01-21 | 2019-08-16 | 华南农业大学 | The method and decapterus maruadsi fish oil of decapterus maruadsi fish oil are extracted in a kind of low temperature continuous phase transistion |
| CN106359535A (en) * | 2016-08-30 | 2017-02-01 | 茂名市海亿食品有限公司 | Fresh-keeping processing method of trachinotus ovatus |
| CN108244273A (en) * | 2016-12-29 | 2018-07-06 | 丰益(上海)生物技术研发中心有限公司 | A kind of fat or oil composition and preparation method thereof |
| CN109588500A (en) * | 2018-11-26 | 2019-04-09 | 郑州远洋油脂工程技术有限公司 | One kind being rich in DHA, EPA human nutrition functionality edible oil-and-fat composition |
| CN110089581A (en) * | 2019-05-31 | 2019-08-06 | 江南大学 | A kind of fat or oil composition |
| CN111700125A (en) * | 2020-06-16 | 2020-09-25 | 江南大学 | Application of pomfret oil or its fraction in preparing human milk substitute lipid |
-
2020
- 2020-06-16 CN CN202010545637.4A patent/CN111700125A/en not_active Withdrawn
- 2020-12-14 CN CN202011477848.5A patent/CN112369472B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112369472A (en) * | 2020-06-16 | 2021-02-19 | 江南大学 | Application of pomfret oil or its extract in preparation of breast milk substitute fat |
| CN112369472B (en) * | 2020-06-16 | 2021-12-28 | 江南大学 | Application of pomfret oil or its extract in preparation of breast milk substitute fat |
| CN112522330A (en) * | 2020-11-27 | 2021-03-19 | 江南大学 | Medium-long carbon chain triglyceride for breast milk to replace fat and preparation method thereof |
| CN112841440A (en) * | 2021-02-02 | 2021-05-28 | 江南大学 | Pig milk fat-replacing composition based on triglyceride |
| WO2022174606A1 (en) * | 2021-02-22 | 2022-08-25 | 北京三元食品股份有限公司 | Infant formula milk powder rich in structured lipid opl, and preparation method therefor |
| CN115644262A (en) * | 2022-05-05 | 2023-01-31 | 上海交通大学 | Grease composition and application thereof |
Also Published As
| Publication number | Publication date |
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| CN112369472A (en) | 2021-02-19 |
| CN112369472B (en) | 2021-12-28 |
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