JPH01502345A - Method of preparing oil composition - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] Method for adjusting oil composition Technical field The present invention relates to Hypopthalmichitis and has high omega-3 unsaturated fatty acids from the species Concerning the preparation of oil compositions.

Technical background Arteriosclerosis and its effects, such as myocardial infarction caused by coronary artery occlusion, , one of the most frequent causes of death in developed countries. Its increasing frequency is common characteristics of aspects of life and nutrition that increase their burden and risk by known means. Attributable to characteristic changes.

In the nutrient method, substantial changes and distortions in the amount and composition of fatty substances occur in large cities. Compare the dietary habits of people living in the natural environment with the diet of people living in the natural environment. can be observed in some cases. A well-known consequence of this compositional change is hyperlipidemia, To prevent arteriosclerosis, which is associated with increased cholesterol levels in the blood, Screening controls have been introduced in many countries.

Over the past decade, the average distribution of unsaturated fatty acids in the blood has become even more important. It has been found that this plays an important role.

These fatty acids are compounds that are metabolized in living organisms (such compounds have important regulatory functions). If a food that is a precursor to The resulting functional balance of regulatory compounds can become unequal, leading to health problems. lead to significant impacts.

The above phenomenon shows that arteriosclerosis, hypertension, and thrombosis occur in the Niskimo people and in some Japanese fishing villages. Supported by the fact that it is virtually undetectable in people. Genetic reasons have been ruled out; The answer to this difference can be explained by a very characteristic method of training that is rich in fish. It was done. Niskimo people consume 700 fish per year. However, the consumption of Americans is 7 per year, and the consumption of Hungarian rum is 7 per year. It is 3 kg per unit. These fish types are also very important: i.e. , they are plant-feeding cold-sea fishes that are rich in fat, and their fat composition is , depending on its omega-3 unsaturated fatty acid content [^-, J, Cl1n , Nutr, 28.958 (1975), Lancetl.

117 (1978), Lancet 2.433 (1979)).

Fatty acids exist in living organisms in the form of phospholipids, triglycerides and free fatty acids. So According to their origin and function, they have a need that can be divided into four main groups. Saturated fatty acids: They can be metabolized by any organism from any carbon source through acetic acid . They exist in human blood mostly as animal fat.

Mono-omega-9 unsaturated fatty acids: The characteristic starting material of this group is oleic acid. It sign table 1,000-50,234 5 (2) are synthesized by plants and animals.

Mono-omega-6 unsaturated fatty acids: The characteristic starting material of this group is linoleic acid. They are found in plant tissues. That is, it exists in seeds.

-Omega-3 unsaturated fatty acids: A characteristic starting material of this group is lylunic acid. they live in water It is synthesized by microorganisms that feed on said organisms, and is deposited in the tissues of fish that feed on said organisms. human As food, they can only be ingested by eating fish, but only Only a small amount of it is taken into living organisms. The importance of this small amount is This is evidenced by the fact that it exists in brain tissue. They are non-essential compounds However, in its absence, dysregulation and disease occur, and indirect and other Its deletion together with the factor may even cause death.

Fatty acids are classified as X:Y omega Z (number of carbon atoms: number of double bonds and Z means the length of the saturated carbon chain after the last double bond, including the terminal methyl group) .

The above fatty acids are classified as follows: 18:1 omega-9 = oleic acid; 18:2 omega-6 = linoleic acid; 18:3 omega-3 = lylunic acid.

The C2° group of fatty acids includes eicosanoic acids, such as 20:4 omega-6 ara Groups of chydonic acid (AA) and 20:5 omega-3 eicosa-pentanoic acid (EPA) It's a loop.

The CO fatty acid group includes tocosanoic acids, such as 22:1 omega-amino acids, among others. In the group of 9 erucic acid and 22:6 omega-3 docosahexanoic acid (DHA) be.

In the formation of the above regulators, C2° eicosanoic acid plays an important role and The group of C2° compounds thus obtained was named as eicosanoids. be done. The functional derivatives obtained therefrom are ring-closed (Proc, Natl, ^ca d, Sci, 1975° Himberg). and prostaglandins. Although the latter group is already known so far , but it was recently discovered that they are derived from eicosane (Nature, (1976, Honcacla), leukotrienes are also (Proc, Natl, ^cad, Sci, 1979゜Mur phy), and even today the metabolites and functions have become known.

The eicosanoid biosynthesis pathway is illustrated in FIG. Its natural starting material is During ring closure, arachidonic acid (AA), which can be found in lipids, forms two double bonds. The bond is cleaved and delta-5, delta-14-dienes are formed. Therefore, This group is also named as "diene metabolites".

The functions of the metabolites illustrated in Figure 1 are as follows: Vasoconstrictor, A DP-opening, platelet activation, increased adhesion of activated platelets, and increase in vascular wall cells. Hormones that stimulate reproduction will be produced; they also release serotonin. and promotes the production of thromboxane.

PXA: Stimulates platelets and promotes thrombus formation.

LTBi: induces inflammation, stimulates immune response.

12HETE: Chemotactic effect on leukocytes.

Fi,: vasodilator.

l and iyu lxyuruo: By-products secreted from living organisms.

Therefore, the eicosanoid system is susceptible to damaging influences, such as the invasion and invasion of foreign substances. inflammation, allergic reactions, thickening of the blood vessel walls, and vascular constriction. It reacts with shrinkage and obstruction of blood flow in the area of injury.

The basic principle of each adjustment in response to its effects is suppressed within a certain range. The activated regulatory system, on the other hand, is responsible for the response or undesired Orient that condition in the direction.

In this case, the organism uses eicosapentanoic acid (EPA) and its derivatives in the same enzyme system. The stem responds as if it were a similar substrate. These molecules are similar to AA metabolites. compete with the corresponding member of the and push the former to the surface of the enzyme, but its Their own metabolites are virtually ineffective.

The two starting materials and their metabolites differ from each other in the delta-17 double bond. . Metabolites of EPA origin are referred to as "triene" metabolites. Therefore, the biological barrier Lance depends on the equilibrium of diene versus triene metabolites (Nature 307, 165.1984).

The biosynthetic pathway derived from EPA is shown in FIG.

The functions of the metabolites shown in Figure 2 are as follows: l: Slightly vasodilatory; Ru.

Animal h: Vasodilatory properties substantially the same as PCl3.

The triene metabolite is a cycle that inhibits phospholipase A2 and thus the formation of AA. Increase Rick AMP level.

22:6 omega-3 docosahexanoic acid (DMA) has the same effects as EPA. has. Omega-3 acids with a low number of carbon atoms are It is converted to EPA in living organisms. The metabolic pathways and functions of metabolites are For example, it is described in the following documents: Proc, Natl, ^cad, Sei, , 76°944 (1979); B, B, ^, , 875.369 (1986) 　;　New Englancl　J,　ofWed,, 314.937(19 86); Thrombosis Res, 42.99 (1986); Nutr, Reviews, 44.205 (1986).

Biochemical tests, animal and clinical experiments show that the distribution of unsaturated fatty acids is permanently high. , the ratio of omega-6 to omega-3 is associated with vasoconstriction, thrombosis, allergies and Proven to increase susceptibility to inflammation. By correcting the above ratios, respectively The disease can be prevented and the symptoms of the disease can be suppressed.

In order to correct this ratio, administration of omega-3 unsaturated fatty acids is necessary to improve common dietary habits. Fish meat or compositions rich in omega-3 unsaturated fatty acids, which may be necessary under The so-called VIDL and LDL fractions favorably influence the lipid content and the HLD reduced to the fraction, and its symptoms reduce cholesterol accumulation, and Seen to promote secretion (Prog, Lipid.

Res., 25.461 (1986)), and other experiments show that the modified The ratio of Omega-6 to Gar-3 is an important factor in the treatment of certain skin diseases. [^rch, Dermatol, 122°1277 (198 6)) Furthermore, research in the field of malignant growth and metastasis has also focused on modified fatty acid fractions. Proved its success (Prog, Lipid.

Res, 25.58:11 (1986)).

The findings suggest that the consumption of cold-water fish such as sardines, mackerel, mackerel, cod, and herring strongly encouraged research and assistance to improve Some freshwater fish also contain omega- The species in question, which has also been shown to be a source for 3-unsaturated fatty acids, is 7 Cyprinus carpio with a fat content of %. , where the EPA content is 4.0% of the total fat content and the AA value is 1.3. % (Lancet, 717.1983 and Prog.

Lipid, Res, 25.207 (1986)).

^quaculture Hungarici 1.35 (1978) paper is , regarding the fatty acid content of Hypopsalmititis species widely bred in Hungary. The paper reporting the trial also reports on the effects of food supply. According to the results, The EPA-content in the label amounts to 8.3% or 9.9% of the total fat content Ru. Its corresponding AA-values were found as 7.5 and 8.5%, respectively.

Food law experts believe that excessive fish consumption may cause the chemicals to accumulate in the fish in the first place. Point out that sea urchin causes insecticidal toxicity. For this reason, and cannot be played The manufacturing and marketing of compositions containing omega-3 unsaturated fatty acids Sales were first introduced in the United States. These are most often Contains 20-50% omega-3 unsaturated fatty acids (EPA and DMA) It is an encapsulated oil.

However, these possibilities have fundamental implications for modifying the diet. It didn't last. As is well known, long-term consumption of certain fatty acids can lead to Harmful. Therefore, C2° and C2□ monoenoic fatty acids are characteristically accumulated in the myocardium. accumulation, thus leading to progressive deterioration and impaired stimulus transmission, i.e. pathological EKG. wake up

C22 erucic acid is present in large amounts in rapeseed oil. Today, machete without erucic acid Significant efforts are being made to improve lipids (Lipids, 746 and 548 (1972)).

Oil compositions available in 1986 have not been analyzed in this regard. tests provided unfavorable results.

Some of those examples are illustrated below: EPA Df1^2 0:1 and 22:1 Cod Liver 11 10% 11% 24% 5 UPER-EPA 30% 20% 11% LOVITRON 9% 11% 20% Additional compositions, such as PROMEG^, PROTOCBOL or ONE^-3- EP^ contains 55-86% of mixed ballast material in addition to the active substance.

The present invention is to provide a solution to eliminate the above harmful effects.

Oiyu @ Takumi Mame The above objectives, which are of great importance for both daily health and medical treatment, It has been found that this can be achieved without having any additional harmful effects on the object. high It has a high content of omega-3 unsaturated fatty acids and 20:1 and 22:1 fat content. Acid-free preparations are available for wild or domesticated Hypopsalmititis sp. Curves (H, molitry (Valucianne)) and big head caps H. nolibis (Richardson) and certain Thai species (A. Bramis), preferably the belly of Abramis bluff (^brasoisbraw&) They may be prepared by using intraluminal fat as a starting material, respectively. Contains lipids extracted therefrom by organic solvents, solvent mixtures, boiling water or steam, and thus The resulting lipid mixture forms a lipid-urea complex and separates it. It is thereby enriched in higher unsaturated fatty acids.

Hybopsarmititis and Avramis species have 2o:1 and 22:1 fat content, respectively. Essentially free of fatty acids and characterized by relatively high EPA- and DHA-content do. The fat content of the muscles is low (3%), i.e. in the saltwater fish listed above. approximately 1/10 of the fat content, but a substantial amount of the fat deposits were previously Can be found on processed offal. The adipose tissue separated from the intestine and omentum is In addition to 100% fat content, surprisingly and preferably elbow-low AA - The main part of fatty acids consists of saturated fatty acids and oleic acid, and the amount is substantially reduced by urea-complex formation and thus the active ingredient content (E PA, DHA and CI8 omega-3 fatty acids converted to polyene EP^) are 6 It can be increased to 0%.

The purification method by forming a urea complex is well known from the lipid literature for CB , P, Kaufman: ^na1gse der Fette undF ettproduct, Band 1.76 pages, Springer V erlaB (1958)).

The method suggests that highly unsaturated fatty acids do not contain urea in the complex, but monoenes or Based on the recognition that saturated fatty acids form complexes with urea. The complex is precipitate from the stem.

This method is used to treat saltwater fish oil according to US-PS No. 4,377.526. used for By this method, EPA of volumetric purity (75-93%) is obtained, in which urea concentration is carried out as a first or second step. of a single ingredient Separation methods include fractional distillation, fractional crystalline molecular distillation at low temperatures or ions on silver-saturated resins. Mention may be made of exchange chromatography.

The method of the invention has the following advantages: - The species Hybopsarmititis is a simple species of fish that can reproduce well.

- By using the adipose tissue, the amount to be extracted is reduced by a factor of 10, - By using the adipose tissue, most of the waste material that can be disposed of is utilized. i.e. substances harmful to the environment are used; Characteristic lipid compositions of one starting material can be obtained using a simple one-step purification method. , ensuring that we obtain a composition with a high active ingredient content and free of harmful substances. - Breeds (Abramis) can also be industrially harvested and require special husbandry. do not need.

The composition obtained according to the present invention can be used to improve food quality and health. 5 (4) act for the management of It has a high active ingredient content (EPA 10 B^) and and preferably low AA-content, and even trace amounts of 20:1 and 22:1 acids cannot be found in them.

A further advantage of the method of the invention is that the lipid extraction is performed using (substantially less than the amount of fresh or whole fish).

The crude product obtained can be effectively purified by a simple one-step method.

The composition prepared according to the present invention is effective against vasoconstriction, arteriosclerosis, vascular occlusion, and allergy. - Used by mixing in food to prevent dermatitis and tumor metastasis. or encapsulated. The composition also provides the correct background in case of said diseases. It can also be used to adjust the method. Daily dosage depends on its symptoms and duration of treatment Depending on the amount, it can vary from 0.2 to 2.0 g.

Ward mBd Kai 1 FIG. 1 shows the biosynthetic pathway of eicosanoids.

Figure 2 shows the biosynthetic pathway starting from EPA.

FIG. 3 shows the fatty acid composition of the starting oil in Example 9.

FIG. 4 shows the analysis of the fatty acid mixture obtained in Example 9.

T Meno Example 1 When handling a 3 year old silver curve (pond bred and weighing 1.2 kg), the internal was removed, and the thick adipose tissue adhering on the internal organs was separated by hand. one fish The average weight of the tissues obtained was 135 g. 1 each from adipose tissue and meat g samples were taken and extracted three times with 5 mff1 petroleum ether each. As a result, the tissue was placed in the presence of 2 g of dry sodium sulfate along with the first solvent loading. Homogenize on the bottom, remove the solid part, and filter each of the 5 stones on the filter. Suspended twice with oil ether. The three solvent fractions were combined and the oily residue Evaporated in vacuo until obtained. The oil was subjected to gas chromatography.

The results obtained were compared with those obtained from carve meat extracts.

Fatty acid silver curve curve from the abdominal cavity 14:0 3,0 1. F 1,6 16: 0 16, 1 18, 3 17,018: 0 0, 4 4, 6 5 ,0 16:9 omega-97,95,2f, 018: 1 45,4 12,6 5 4,718:2 Omega-61,52,66,322: 5 0,5 3,3 0 ,2 18=3 omega-31,44,30,718: 4 nyom O,2 20:4 20: 5 4,6 12,4 0,722: 5 0,7 2,4 0.1 Fat from the intraperitoneal cavity is free of 20:1 and 22:1 fatty acids and had a low AA content. EPA- and DHA content, their content in meat Although relatively small compared to the amount, in terms of the total weight of tissue to be extracted, The effective EPA- and DHA content of the meat is about 1/10 that of adipose tissue. there were.

Example 2 Treated a 3 year old big head curve (weight 2100g). Incision and then After removing the viscera, the adipose tissue over the viscera was separated manually, and thus 200 g of tissue was removed. Obtained. Samples of 1 g each were taken from the adipose tissue and the fish flesh, and Example 1 was extracted as described and analyzed by gas chromatography.

Compare the results obtained with those of compressed oil from a whole saury. did.

Fatty acid big head curve saury from the abdominal cavity 16: 0 20,1 17,4 15,118: 0 2,4 6,1 2,316:1 omega-913,53,43,918:1 31,5 10, 0 13,820: 1 1, 1 0, 9 8, 4 22: 1 10,5 18:2 Omega-61,72,81,120:2 0,2 0,2 20:3 0,9 0.3 20:4 1,0 8,9 0,8 22:4　o,s 22:5 0,1 2.3 18:3 Omega-31,93,81,418:4 0,2 3,3 20:4 0,7 0,3 20: 5 6,6 14,3 7,022: 5 1,4 2,4 0,6 22: 6 2, 4 16, 7 10.5 Bit head curve from intraperitoneal cavity The fatty acid composition of the adipose tissue is similar to that of the silver curve, and the fat composition of the meat is , with higher AA-content and EPA- and DHA-content percentages.

However, when using meat for oil extraction, the weight of the substance to be extracted is There were many again.

In the case of Pacific saury, it constitutes a good source of EPA and DHA, but its products High 20:1 and 22:1 contents were detected.

A3 100 g of adipose tissue obtained according to Example 1 was transferred using a high-speed experimental homogenizer. For example, using an MSE homogenizer, incubate two layers of chloroform at n=8000 rpm for 3 minutes. Homogenized with 2000 g of a mixture of form and methanol (2:1, v/v) did. The emulsion thus obtained is allowed to stand for 3 hours, then a precipitate forms Remove the protein by passing it over -hat+++an IPS paper. , and 0.2M potassium chloride aqueous solution 400 was added to the r solution. That scene Separate the stem into two layers and, after completion of the separation, separate the lower phase containing lipids. , and discarded the upper phase. The chloroform solution was poured over dry sodium sulfate. It was dried, filtered and the solvent was distilled from the P solution. colorless or slightly yellow Colored oils 83.2. and the fatty acid composition is the composition obtained according to Example 1. It was identical to the object.

Example 4 100 g of adipose tissue obtained in Example 2 was homogenized in the presence of 200° dry sodium sulfate. 0 tissue residue extracted with 800 ml of petroleum ether by clarification and The salts were removed, the precipitate was washed with 100 ml of 2× petroleum ether, and The purified material was combined with the extract. Vacuum distillation of the solvent from the lipid solution thus obtained This gave 91 g of a slightly yellow oil. The fatty acid composition obtained in Example 2 The product was identical to that of the prepared product.

Example 5 In processing 1 year old silver curves, adipose tissue was collected intraperitoneally. that thing The material is stored in a plastic container filled with ice and extracted at the temperature of the melting ice. I moved it to its original location.

5000 g of adipose tissue was placed in a 201 stainless steel container equipped with a Turrax stirrer. Add water 51 at 70-80°C, and let the mixture homogenize for 30 minutes. Add 2 x 500 ml of water to the boiled water and mix after each addition. Stir the mixture for a few seconds. The device is cooled by water flowing through the cooling cap. did. After the temperature drops to 30 °C, until an oily layer appears on the surface of the aqueous suspension. , the material was allowed to stand. By sucking up the oily substance with a decant pipe, Remove the oil and discard the oil-free aqueous phase. Dry by separating and thus 4650 g of slightly yellow substance Don't get it.

Example 6 Three sea bream (Abramis bluff), each weighing 250g, were Internal organs were removed from the cavity and adipose tissue was collected.

30 g of adipose tissue was obtained and this was incubated in the presence of 100 ml of petroleum ether. It was crushed using a board homogenizer.

Strain the homogenate and dry the liquid over dry WLfm sodium. 0 petroleum ether was vacuum distilled and 25 g of colorless petroleum ether thus obtained Methylate an aliquot of the fat sample and analyze its fatty acid composition with gas chroma. Determined by topography. The analysis data was used to analyze salmon from the North Sea. The triglyceride content isolated from 5alar) was compared.

Muscles from the abdominal cavity fat triglyceride 14: 0 6, 6 6.6 16: 0 11, 9 9.0 18:Oo,s　O,2 16:1 Omega-912,56,3 18:1 Omega-920,621,3 20:1 Omega-91,221,3 22:1 Omega-913,5 18:2 Omega-67,94,1 20:2 Omega - 60,20,2 22:4 Omega-60,6 18:3 Omega-37,31,0 18:4 Omega-32,8Z,7 20:4 omega - 30,60,6 20:5 Omega-38,34,8 22:5 Omega-31,51,4 Example 7 In the oil obtained from any of Examples 3, 4 or 5, the oil is Free fatty acids by dissolving in twice the volume of petroleum ether, and present The same amount of ethanolic potassium hydroxide as the fatty acid was added thereto. That scene Triglyceride saponification occurs during a period of time in which the stem is left undisturbed at ambient temperature for 10-16 hours. After about 12 hours, when no reaction occurs, add the same amount of water to the reaction mixture, mix, and add the same amount of water to the reaction mixture. Separate the layers by standing or separating, discarding the upper petroleum ether layer, and The lower layer is decomposed by acidifying it to pH=4.0 by adding hydrochloric acid. The free acids, including potassium salts of fatty acids, are dissolved in petroleum ether from their aqueous solutions. Extract and then distill the solvent to obtain an oil.

Example 8 From the fatty acids in the oils obtained from Examples 3, 4 and 5, methyl ester Add sodium methoxide dissolved in half the volume of methanol to the oil Added sodium methoxide is formed by adding fatty acids to The reaction mixture, which was present in an equimolar amount as the content, was allowed to stand at room temperature for 10 to 16 hours, and Then <10 times the volume of petroleum ether based on the fatty acid and the amount necessary for separation of the layers. Water was added. Discard the lower layer, wash the petroleum ether solution with water, and The medium was distilled. The oil thus obtained contains fatty acid methyl esters. Inclusive.

Example 9 Free fatty acids obtained in Example 7 or Example 8 to enrich for higher degree of unsaturation fatty acids The fatty acid methyl ester obtained in Prepare a 20% methanol solution from the oil material and add to it the fatty acid molecules present. Based on the volume, 20 times the amount of urea dissolved in methanol was added. final solution fat The reaction mixture, with the amount of methanol selected to give a fatty acid concentration of 10%, was It was incubated at 5° C. for 24 hours. During this period, the main amounts of saturated mono- and diene fats, respectively, Urea and adducts from which fatty acids and fatty acid methyl esters crystallize was formed. The crystals were removed at the same temperature with cooling. Add the P liquid to water The polyenoic fatty acids were extracted into petroleum ether and the solvent Figures 3 and 4 show the results of hot water extraction, saponification and production. Figure 3 shows urea purification of fatty acids obtained from adipose tissue with a more silver curve. , which shows the fatty acid composition of the starting oil, shows that by adduct formation according to the invention, Figure 2 shows an analysis of the resulting polyene-enriched fatty acid mixture.

To preserve adipose tissue, optionally strong freezing or antioxidants, such as 0. Addition of 1% tocopherol or 0.05% butylated hydroxytoluene was performed. It can be done. Solvent extraction is carried out, for example, in a backflow extractor, and aqueous extraction It can be done in an autoclave by steaming the adipose tissue.

Free fatty acids can also be converted to water by enzymatic decomposition, e.g. Mfrylated by lipase. can be produced in a sexual medium.

Urea complex formation is performed by heating at 50-70°C for 30-60 minutes. be exposed. To precipitate the adducts, cool the system to below 30°C. .

Fatty acids can also be used in other fatty solvents such as n-hexane, benzene, ethyl acetate, etc. It can be extracted from its aqueous solution by a method, etc. Before distilling the solvent, water, e.g. Those containing ethyl acetate should be dried by sodium sulfate, resin treatment, etc. It is.

PL AA (So>!iFX) Fi;i PLEPA 9'v-〇 ■? L0 FXG, 岑 Procedural amendment March 17, 1999 Yoshi, Commissioner of the Patent Office 1) Takeshi Moon 1.Display of the incident PCT/HU88100027 2 Name of the invention Method of preparing oil composition 3. Person who makes corrections Relationship to the incident: Patent applicant Name Mteer Szegedi Biologia Ikespontoya 4. Agent Address: 8-10 Toranomon-chome, Minato-ku, Tokyo 105 & Subject of amendment Specification 6. Contents of amendment (1) (Ω　Page 8, line 23 of the specification, replace ``for both'' with 1 for both. Correct.

(o) Page 10, line 18 of the same page, "can be used" cannot be obtained as r, and is amended to J. .

Shi ~ Page 16, lines 22-23, “(each weighing 250g).” is corrected to j of (each weighs 250 g).

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Claims (6)

[Claims] 1. A nutritional supplement containing omega-3 unsaturated fatty acids by extracting lipids from fish. A method for preparing a foot composition, the method comprising: (Abramis) and optionally its extract for urea complex formation. A method characterized by further purification. 2. Claim 1, characterized in that Hypopsalmititis species is used. Method. 3. Claim No. 1, characterized in that the bred Hypopsalmititis sp. The method described in Section 2. 4. Claims characterized by using a silver curve or a big head curve The method described in any one of Boxes 2 and 3. 5. Claim 1, characterized in that Abramis brama is used. How to put it on. 6. Claim characterized in that fish intraperitoneal fat is used as starting material for extraction The method according to any one of items 1 to 5.