CN112369472B - Application of pomfret oil or its extract in preparation of breast milk substitute fat - Google Patents
Application of pomfret oil or its extract in preparation of breast milk substitute fat Download PDFInfo
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Abstract
The invention discloses application of pomfret oil or a fractionation product thereof in preparation of breast milk substitute fat, and belongs to the technical field of foods. The natural oil and fat rarely contain UPU type triglyceride, and a few natural oil and fat are rich in OPO, but the content of OPL is very low. The highest content of triglycerides in golden pomfret oil is OPL, and the second is OPO, which is similar to the triglyceride composition of Chinese mother butterfat. Golden pomfret oil, freshwater white pomfret or the fractionated matters thereof are selected from pomfret oil as base oil, and other natural grease or modified grease is added to compound to obtain breast milk substitute grease. The milk substitute fat has high similarity with milk fat, solves the problem of low absorption rate of fatty acid and calcium of infant formula milk powder, improves the absorption and utilization of energy, improves the hardness of feces, and reduces the occurrence of constipation and intestinal diseases of infants.
Description
Technical Field
The invention relates to an application of pomfret oil or a fractionation product thereof in preparation of a breast milk substitute fat, and belongs to the technical field of foods.
Background
The breast milk is the best food for infants and can provide all nutrients required by the infants for growth and development. The lipid component in breast milk, called as breast milk fat, accounts for about 3-5%, provides 40-50% of energy and essential fatty acids for infants and young children, and can promote the absorption of fat-soluble vitamins. Milk fat is a complex mixture of fats and oils, and the triglycerides in milk fat are mostly present in the sn-UPU form, such as 1, 3-dioleoyl-2-palmitoyl triglyceride (OPO) and 1-oleic-2-palmitic-3-oleic triglyceride (OPL). The unique distribution of fatty acids on the triglyceride backbone affects digestion, absorption and metabolism in infants. Many studies have shown that feeding breast milk fat is beneficial for infants to absorb fat and calcium, while feeding most natural fats causes problems such as poor fat absorption and calcium loss in infants, and that feeding breast milk fat prevents constipation in infants, whereas ingestion of natural fat generally causes constipation in infants as a human milk fat animal, Journal of agricultural and Food Chemistry,2011,59, 5692-.
With the development of society, the employment opportunities of women are increased, the unnatural fertility rate is increased, and the breast-feeding rate of puerperae is continuously reduced due to various reasons such as insufficient understanding and self condition of breast-feeding. Therefore, infant formula has become the best substitute food, and its lipid component is called breast milk substitute fat (HMFS). According to a large amount of literature reports, the content of OPO in the milk fat of mother milk in many countries and regions is the highest, but the OPL is found in many regions in China to be the triglyceride with the highest content in the milk in China, and the OPO is the second highest. At present, most of the breast milk substitute fat in the market is developed based on the composition of foreign breast milk fat, the components of the breast milk fat of Chinese people are not concerned, and the addition of OPL is not noticed in infant formula milk powder, so that the difference between the breast milk substitute fat in the market of China and the breast milk fat of Chinese people is large, and the growth and development of infants are not facilitated. The Sungclen analyzes the composition of breast milk substitute fat in 180 infant formula milk powder in the Chinese market, compares the composition with the composition of milk fat of Chinese mother milk, and scores the composition, and the result shows that the average total similarity coefficient (G) of the breast milk substitute fat of 180 milk powder and the Chinese breast milk fat is 35.72, and the similarity of 82.78% of the breast milk substitute fat of the milk powder is distributed between 20 and 50 minutes (Sungclen. the composition, similarity evaluation and preparation research of the breast milk substitute fat [ D ]: Boshi academic thesis ]. Wuxi: Jiangnan university, 2018), which indicates that the breast milk substitute fat in the current Chinese market has great composition difference with the Chinese mother milk fat, and needs to develop and continuously optimize the breast milk substitute fat which is more similar to the Chinese breast milk fat
The natural oil is rarely rich in UPU type triglyceride, and the Basha fish oil and the Black fish oil only contain a certain content of OPO, and the content of OPL is still low, which is not consistent with the breast milk fat composition of Chinese people. When only the black fish oil or the Bassa fish oil is used for blending, the problem of insufficient OPL in the oil for the infant milk powder cannot be solved, and the similarity with breast milk fat is low. In addition, the synthesis of OPL by enzymatic methods is less studied and expensive. Therefore, a new oil rich in OPL is needed to be found and applied to preparing breast milk substitute fat.
Disclosure of Invention
[ problem ] to
The invention aims to solve the technical problem that the similarity of the existing breast milk substitute fat to the breast milk fat in terms of sn-2 fatty acid composition and triglyceride composition is low.
[ solution ]
The invention finds that golden pomfret oil and freshwater white pomfret oil (Colossoma bracho ypomum), wherein the golden pomfret oil comprises Trachinotus ovatus oil (Trachinotus ovatus), Trachinotus buchneri oil (Trachinotus blochi) and the like, is rich in OPL and OPO, has fatty acid composition similar to that of breast milk, is the closest to that of breast milk of Chinese people, is a better choice for formula milk powder for infants in China, and has application potential in the aspect of replacing the fat with the breast milk.
The invention provides application of pomfret oil or pomfret oil extract in preparation of milk substitute fat or milk products.
Further, the pomfret oil comprises one or two of golden pomfret oil and freshwater white pomfret oil.
Further, the golden pompano fish oil is egg-shaped pompano fish oil or pompano fish oil.
Further, the pomfret oil is obtained by extraction through a solvent method or an aqueous enzymatic method.
Further, the pomfret oil extract is obtained by performing fractionation on pomfret oil by adopting a dry fractionation method or a solvent fractionation method.
Further, in the preparation method of the pomfret oil fraction, an extraction solvent extracted by a solvent method is acetone or normal hexane; the mass-to-volume ratio (w/v, g/mL) of the pomfret oil to the solvent is 1: (2-10); the extraction temperature is-30-0 ℃; the time is 10-24 h.
Further, the pomfret oil or pomfret oil extract is used as an OPO and OPL supplementary ingredient in preparation of mother milk substitute fat.
Further, the pomfret oil or pomfret oil extract is preferably golden pomfret oil or golden pomfret oil fraction.
The invention provides a breast milk fat-replacing composition, which is prepared by taking pomfret oil or pomfret oil extract as base oil and adding other natural oil and/or modified oil.
Further, in the breast milk substitute fat composition, the sn-2-position palmitic acid accounts for 38-60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 23-50% of the total triglyceride content in percentage by mass. Further, preferably, in the breast milk substitute fat composition, the content of the palmitic acid at the sn-2position accounts for 50-60% of the total content of the palmitic acid, and the sum of the OPO and the OPL accounts for 35-50% of the total content of the triglyceride.
Further, the natural oil comprises one or more of soybean oil, peanut oil, palm kernel oil, coconut oil and basha fish oil.
Further, the modified grease comprises one or more of OPO structure grease, OPL structure grease and medium-long chain triglyceride (MLCT) structure grease.
Further, the OPO structural lipid is a structural lipid with an OPO content of more than 40% of the total triglyceride content; the OPL structural lipid is structural lipid with OPL content accounting for more than 40% of the total triglyceride content, and the MLCT structural lipid is structural lipid with MLCT content accounting for more than 40% of the total triglyceride content.
Further, the breast milk substitute fat composition includes: 1-50% of natural oil or/and modified oil and 50-99% of pomfret oil or pomfret oil extract by mass percentage.
Further, the breast milk substitute fat composition includes: 1-50% of natural oil and 50-99% of pomfret oil or pomfret oil extract by mass percentage.
Further, the breast milk substitute fat composition includes: 1-40% of coconut oil or/and palm kernel oil and 60-99% of golden pompano oil or golden pompano oil fractionation substances by mass percentage; preferably comprising: 10-20% of coconut oil or/and palm kernel oil and 80-90% of golden pompano oil or golden pompano oil fractionation substances;
or 10-20% of coconut oil or/and palm kernel oil, 1-20% of palm oil and 60-89% of golden pompano oil or golden pompano oil fractionation substance;
or 10-20% of coconut oil or/and palm kernel oil, 0-10% of soybean oil, 0-10% of peanut oil, 0-10% of palm oil, 20-30% of sandfish oil and 50-70% of golden pompano oil or golden pompano oil extract;
or 0-10% of soybean oil, 0-10% of peanut oil, 0-10% of palm oil, 10-20% of coconut oil, 40-50% of sandwort fish oil and 30-45% of golden pompano oil or golden pompano oil extract.
Or 10-20% of coconut oil or/and palm kernel oil, 10-20% of soybean oil, 0-5% of peanut oil, 0-5% of palm oil and 60-80% of freshwater white pomfret oil or freshwater white pomfret oil extract.
Further, the breast milk substitute fat composition includes: 1-40% of natural oil, 1-20% of modified oil and 50-98% of golden pompano oil or golden pompano oil fractionation substances by mass percentage;
or 10-20% of coconut oil or/and palm kernel oil, 1-20% of OPO structure fat and 60-89% of golden pompano oil or golden pompano oil fractionation substance; preferably comprising: 10-20% of coconut oil or/and palm kernel oil, 1-5% of OPO structure fat and 75-89% of golden pompano oil or golden pompano oil fractionation substance;
or 10-20% of coconut oil or/and palm kernel oil, 1-20% of soybean oil, 1-20% of OPO structure fat and 60-80% of golden pompano oil or golden pompano oil fractionation substance; preferably comprising: 10 to 20 percent of coconut oil or/and palm kernel oil, 1 to 5 percent of soybean oil, 1 to 5 percent of OPO structure fat and 70 to 88 percent of golden pompano oil or golden pompano oil extract;
or soybean oil 1-20%, palm oil 1-20%, MLCT structural fat 1-20%, and golden pompano oil 60-80% or golden pompano oil fraction; preferably comprising: soybean oil 1-5 wt%, palm oil 1-10 wt%, MLCT structure fat 10-20 wt% and golden pomfret oil 65-88 wt% or golden pomfret oil fraction.
Further, the breast milk substitute fat composition includes: 1-40% of natural oil, 1-20% of modified oil and 50-98% of freshwater white pomfret oil or freshwater white pomfret oil fractionation substances by mass percentage;
or 10-20% of coconut oil or/and palm kernel oil, 1-20% of OPL structure fat and 60-89% of freshwater white pomfret oil or freshwater white pomfret oil fractionation substance; preferably comprising: 10-20% of coconut oil or/and palm kernel oil, 10-20% of OPL structure fat and 60-80% of freshwater white pomfret oil or freshwater white pomfret oil fractionation substance;
or 10-20% of coconut oil or/and palm kernel oil, 1-20% of palm oil, 1-20% of OPL structure fat and 60-80% of freshwater white pomfret oil or freshwater white pomfret oil fractionation substance; preferably comprising: 10-20% of coconut oil or/and palm kernel oil, 1-5% of palm oil, 10-20% of OPL structure fat and 55-79% of freshwater white pomfret oil or freshwater white pomfret oil fractionation substances;
or 0-15% of coconut oil or/and palm kernel oil, 0-5% of soybean oil, 0-5% of peanut oil, 0-5% of palm oil, 0-10% of OPL structural fat, 10-20% of MLCT structural fat and 50-80% of freshwater white pomfret oil or freshwater white pomfret oil extract;
or comprises 0-10% of soybean oil, 0-10% of peanut oil, 0-10% of palm oil, 10-20% of coconut oil, 15-25% of basha fish oil, 20-30% of OPL structure fat and 25-40% of freshwater white pomfret oil or a freshwater white pomfret oil fractionation substance.
The present invention provides a food product comprising the above breast milk substitute fat composition.
Further, the food product comprises milk powder or other milk products, and the milk powder comprises infant milk powder, adult milk powder, middle aged and elderly milk powder and animal baby milk powder.
The invention has the beneficial effects that:
(1) the high content of OPL in the base oil selected by the invention is not found in other natural source grease, and the OPL is the triglyceride with the highest content in Chinese mother milk fat, which is different from the foreign breast milk fat. Experiments show that the contents of palmitic acid, sn-2 palmitic acid, OPO and OPL in golden pomfret oil are 23.52%, 37.25%, 15.84% and 25.67% respectively, the contents of palmitic acid, sn-2 palmitic acid, OPO and OPL in freshwater white pomfret oil are 25.26%, 36.96%, 26.39% and 19.38% respectively, and the two kinds of fish oil both contain high content of OPL and OPO, have composition close to that of breast milk fat of Chinese people, and are excellent base oil for preparing breast milk substitute fat.
(2) The breast milk substitute fat provided by the invention has higher similarity with Chinese breast milk fat in the aspects of the fatty acid composition and triglyceride composition of the sn-2 site. Wherein the similarity score of the fatty acid composition at the sn-2position and the Chinese breast milk fat is more than 80 points, and the similarity score of the triglyceride composition and the Chinese breast milk fat is more than 70 points. The total similarity score is more than 80 points, which is more in line with the requirements of Chinese babies; the invention can also obtain the breast milk substitute fat composed of pure natural fat, can obtain the substitute fat similar to the Chinese breast milk only by combining the natural fat and the golden pomfret oil, and has higher score and lower cost.
(3) Compared with the existing enzymatic method and chemical method for synthesizing related structural grease, the base oil for preparing the breast milk substitute grease provided by the invention has the advantages of simpler process, higher safety and suitability for industrial production.
(4) The milk substitute fat has high similarity with milk fat, solves the problem of low absorption rate of fatty acid and calcium of infant formula milk powder, improves the absorption and utilization of energy, improves the hardness of feces, and reduces the occurrence of constipation and intestinal diseases of infants.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of better illustrating the invention and is not intended to limit the invention thereto.
The invention relates to the determination of the composition of total fatty acids, sn-2 fatty acids and triglycerides according to the method adopted in the Sungsmart doctor paper (the composition, similarity evaluation and preparation research of the Sungsmart breast milk substituted fat [ D ]: Boshiacademic paper, Wuxi: university of Jiangnan, 2018). And (4) completing quantification by a peak area normalization method, calibrating by using a standard substance to convert the area percentage into the mass percentage, and calculating the content.
The similarity of the breast milk substitute fat related by the invention and the Chinese mother milk fat in the aspects of the composition of fatty acid, sn-2 site fatty acid and triglyceride is also evaluated by adopting a 'deduction' principle according to a mother milk fat similarity evaluation model established in Sungcleng doctor paper. The mother milk fat similarity evaluation model is as follows:
wherein G represents the total score of similarity evaluation, and the full score is 100 points; gFA/sn-2FA/TAGA similarity evaluation score representing a fatty acid, a sn-2 fatty acid or a triglyceride; n is the number of indices in the total fatty acids or sn-2 fatty acids or triglycerides; biActual values representing total fatty acids or sn-2 fatty acids or triglycerides of breast milk substitute lipids; a isiThe end value of total fatty acids or sn-2 fatty acids or triglycerides of milk fat of mother milk depends on biThe size of (2).
The OPO structure lipid, the OPL structure lipid and the MLCT structure lipid used in the invention are synthesized by a laboratory enzyme method, wherein the OPO is synthesized by acidolysis reaction of palm stearin and oleic acid under the catalysis of NS40086 lipase, the molar ratio of the substrate is 1: 10, the dosage of the lipase is 10%, the reaction temperature is 60 ℃, and the reaction time is 4 hours; the OPL is synthesized by acidolysis reaction of palm stearin, oleic acid and linoleic acid under the catalysis of NS40086 lipase, the molar ratio of a substrate is 1:7:7, the addition amount of the NS40086 lipase is 10%, the reaction temperature is 60 ℃, and the reaction time is 4 hours; the MLCT structure fat is synthesized by ester exchange of grease rich in OPO and OPL and coconut oil under the catalysis of NS40086 lipase, the molar ratio of substrates is 0.8: 1, the dosage of the lipase is 10%, the reaction temperature is 60 ℃, and the reaction time is 6 h.
The modified oil comprises oil obtained by modifying natural oil by other methods. Other oils and fats are commercially available unless otherwise specified.
Example 1: golden pomfret oil
Extracting golden pompano fish oil (trachinotus ovatus oil) with chloroform-methanol as solvent, freeze drying fresh golden pompano liver, and pulverizing. Taking 10g of the crushed powder, adding 100mL of methanol, shaking, adding 200mL of chloroform, carrying out ultrasonic treatment for 30min, carrying out suction filtration, taking clear liquid, adding 100mL of sodium chloride solution with the mass fraction of 0.88%, centrifuging the mixed solution at 4500rpm for 10min, sucking the lower layer, placing the lower layer in a round-bottom flask, and carrying out rotary evaporation to remove the solvent to obtain the golden pompano fish oil. The contents of total palmitic acid, sn-2-position palmitic acid, OPO and OPL in the golden pomfret oil are respectively 23.52%, 37.25%, 15.84% and 25.67% of the total triglyceride, and the sn-2-position palmitic acid accounts for 52.79% of the total palmitic acid content through calculation. And the content of OPL is higher than that of OPO, and the breast milk substitute fat similar to Chinese mother milk fat can be prepared under the condition of not adding OPL structural fat.
Example 2: freshwater white pomfret oil
Extracting the freshwater white pomfret oil by using normal hexane as a solvent. And taking 10g of the crushed powder, adding 100mL of n-hexane, carrying out ultrasonic treatment for 30min, carrying out suction filtration, placing the clear liquid in a round-bottom flask, and carrying out rotary evaporation to remove the solvent to obtain the freshwater white pomfret oil. The contents of total palmitic acid, sn-2 palmitic acid, OPO and OPL in the freshwater white pomfret oil are respectively 25.26%, 36.96%, 26.39% and 19.38% of the total triglyceride content. The calculated result shows that the sn-2position palmitic acid accounts for 48.77 percent of the total palmitic acid content. Although the content of OPL is slightly lower than that of OPO, the content of OPL is slightly lower than that of golden pomfret oil, but the content of OPL is higher than that of other fish oil, so that a small amount of OPL structural fat can be added to adjust the proportion of OPL and OPO, and the breast milk substitute fat can be prepared.
Example 3: preparation method of fractionated product of golden pomfret oil
And (2) carrying out solvent fractionation on the golden pomfret oil obtained in the example 1, wherein the used solvent is acetone, the ratio (w/v) of the golden pomfret oil to the solvent is 1:8, the fractionation temperature is-25 ℃, and the fractionation time is 16h, so as to obtain a golden pomfret oil fraction. The contents of total palmitic acid, sn-2-position palmitic acid, OPO and OPL in the golden pomfret oil fraction are respectively 27.82%, 45.37%, 20.28% and 29.46%. The calculated result shows that the sn-2position palmitic acid accounts for 54.36 percent of the total palmitic acid content.
Example 4: composition for replacing fat with breast milk
Coconut oil and golden pomfret oil prepared in example 1 are selected as base oil, and similarity scores of total fatty acids, sn-2 site fatty acids and triglyceride in the obtained breast milk substitute fat composition and Chinese mother butter fat are shown in table 1 under different proportions of coconut oil and golden pomfret oil.
TABLE 1 scoring results for breast milk substitute fat obtained with different coconut oil and golden pompano fish oil compositions
As can be seen from Table 1, when 10-20% coconut oil, 80-90% golden pompano oil or golden pompano oil fraction is preferred, the sn-2 palmitic acid in the obtained breast milk substitute fat accounts for 40-60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 30-40% of the total triglyceride content. The similarity scores of the sn-2position fatty acid and triglyceride of the milk fat are respectively over 80 points and over 70 points, and the total similarity score is over 80 points. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 5: composition for replacing fat with breast milk
The palm kernel oil and the golden pomfret oil prepared in example 1 are selected as base oil, and similarity scores of total fatty acids, sn-2 site fatty acids and triglyceride in the obtained breast milk substitute fat composition and Chinese mother milk fat are shown in table 2 under different proportions of the palm kernel oil and the golden pomfret oil.
TABLE 2 scoring results for breast milk substitute fats with different palm kernel oil and golden pomfret oil compositions
As can be seen from Table 2, when 10% to 20% palm kernel oil, 80% to 90% golden pompano oil or golden pompano oil fraction is preferred, the sn-2 palmitic acid in the obtained breast milk substitute fat accounts for 40% to 60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 30% to 40% of the total triglyceride content. The similarity scores of the total fatty acids, sn-2 fatty acids and triglyceride and Chinese mother milk fat are all over 80 points, and the total similarity score is over 85 points. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 6: composition for replacing fat with breast milk
The similarity scores of the total fatty acids, sn-2 site fatty acids and triglycerides in the obtained breast milk substitute fat composition and the milk fat of Chinese mothers are shown in table 3 under different proportions by selecting palm kernel oil, palm oil and the golden pomfret oil fraction prepared in example 3 as base oil.
TABLE 3 scoring results for breast milk substitute fats with different compositions of palm kernel oil, palm oil and fractionated fish oil
As can be seen from Table 3, when the mixture ratio is 10% -20% of palm kernel oil, 1% -20% of palm oil, 60% -90% of golden pompano oil or the fraction of golden pompano oil, the sn-2 palmitic acid in the obtained breast milk substitute fat accounts for 30% -60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 30% -50% of the total triglyceride content. The similarity scores of the total fatty acids, sn-2 fatty acids and triglyceride and Chinese mother milk fat are all over 80 points, and the total similarity score is over 85 points. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 7: composition for replacing fat with breast milk
Coconut oil, soybean oil, palm oil, palatinose butterfish oil and the golden pomfret oil extracted in example 1 are selected as base oil, and the mixture ratio is coconut oil: soybean oil: palm oil: bassa fish oil: golden pomfret oil 18.16%: 0.11%: 0.06%: 25.53%: 56.14% with a palmitic acid content of 34.29% in the fatty acid composition at the sn-2position, calculated as 48.33% palmitic acid at the sn-2position of the total palmitic acid content. The sum of OPO and OPL represents 33.97% of the total triglyceride content. The similarity scores of the total fatty acids, sn-2 fatty acids and triglyceride and the Chinese mother milk fat are 93.79, 83.91 and 81.46 respectively, and the total similarity score is 86.39. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 8: composition for replacing fat with breast milk
Coconut oil, OPO structure fat and the golden pomfret oil extract prepared in example 3 are selected as base oil, and similarity scores of total fatty acids, sn-2 site fatty acids and triglyceride in the obtained breast milk substitute fat composition and Chinese mother butterfat are shown in table 4 under different proportions.
TABLE 4 scoring results of breast milk substitute lipids consisting of different coconut oil, OPO structural lipids and fractionated fish oil
As can be seen from Table 4, when the mixture ratio is 10% -20% of coconut oil, 1% -5% of OPO structure fat, 75% -89% of golden pompano oil or golden pompano oil extract, the sn-2-position palmitic acid in the obtained breast milk substitute fat accounts for 40% -60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 30% -50% of the total triglyceride content. The total similarity score is above 80 points. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 9: composition for replacing fat with breast milk
The similarity scores of the total fatty acids, sn-2 site fatty acids and triglyceride in the obtained breast milk substitute fat composition and Chinese mother butterfat in different proportions by taking palm kernel oil, OPO structure fat and golden pompano fish oil prepared in example 1 as base material oil are shown in table 5.
TABLE 5 scoring results for breast milk substitute lipids obtained with different palm kernel oil, OPO structural lipids and golden pomfret oil compositions
As can be seen from Table 5, when the mixture ratio is 10% -20% of palm kernel oil, 1% -5% of OPO structural fat, 75% -89% of golden pompano oil or golden pompano oil extract, the sn-2 palmitic acid in the obtained breast milk substitute fat accounts for 50% -60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 30% -40% of the total triglyceride content. The similarity scores of the total fatty acids, sn-2 fatty acids and triglyceride and Chinese mother milk fat are all over 80 points, and the total similarity score is over 85 points. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 10: composition for replacing fat with breast milk
The similarity scores of the total fatty acids, sn-2 site fatty acids and triglycerides in the obtained breast milk substitute fat composition and the milk fat of Chinese mothers are shown in table 6 under different proportions by selecting palm kernel oil, soybean oil, OPO structure fat and the golden pompano fish oil prepared in example 1 as base material oils.
TABLE 6 scoring results for breast milk substitute fats with different palm kernel oil, soybean oil, OPO structure fat and golden pompano fish oil compositions
As can be seen from Table 6, when the mixture ratio is 10% -20% of palm kernel oil, 1% -5% of soybean oil, 1% -5% of OPO structural fat, 70% -88% of golden pompano oil or golden pompano oil extract, the sn-2 palmitic acid in the obtained breast milk substitute fat accounts for 40% -60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 30% -40% of the total triglyceride content. The similarity score of the triglyceride and the milk fat of Chinese mothers is above 80 points, and the total similarity score is above 85 points. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 11: composition for replacing fat with breast milk
Soybean oil, palm oil, MLCT structural fat and golden pomfret oil prepared in the embodiment 1 are selected as base oil, and similarity scores of total fatty acid, sn-2 site fatty acid and triglyceride in the obtained breast milk substitute fat composition and Chinese mother butterfat are shown in a table 7 under different proportions.
TABLE 7 scoring results for breast milk substitute lipids obtained with different compositions of soybean oil, palm oil, MLCT structural lipids and golden pomfret oil
As can be seen from Table 7, when the mixture ratio is 1% -5% of soybean oil, 1% -10% of palm oil, 10% -20% of MLCT structural fat, 65% -88% of golden pompano oil or golden pompano oil extract, the sn-2 palmitic acid in the obtained breast milk substitute fat accounts for 40% -50% of the total palmitic acid content, and the sum of OPO and OPL accounts for 30% -40% of the total triglyceride content. The similarity scores of the total fatty acids, sn-2 fatty acids and triglyceride and Chinese mother milk fat are all over 80 points, and the total similarity score is over 85 points. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 12: composition for replacing fat with breast milk
Selecting palm kernel oil, soybean oil, palm oil, MLCT structure fat and golden pomfret oil extracted in example 1 as base oil, wherein the ratio of the palm kernel oil to the golden pomfret oil is as follows: soybean oil: palm oil: MLCT structural lipid: golden pomfret oil 10%: 5%: 5%: 15%: 65% with a palmitic acid content of 32.25% in the fatty acid composition at the sn-2position, calculated as 46.14% palmitic acid in the sn-2position of the total palmitic acid content. The sum of OPO and OPL represents 30.59% of the total triglyceride content. The similarity scores of the total fatty acids, sn-2 fatty acids and triglyceride and the Chinese mother milk fat are 91.13, 85.49 and 96.11 respectively, and the total similarity score is 90.91. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 13: composition for replacing fat with breast milk
The similarity scores of the total fatty acids, sn-2 site fatty acids and triglycerides in the obtained breast milk substitute fat composition and the milk fat of Chinese mothers are shown in table 8 under different proportions by selecting palm kernel oil, OPL structural fat and the freshwater white pomfret oil prepared in example 2 as base oil.
TABLE 8 scoring results for breast milk substitute lipids from different compositions of palm kernel oil, OPL structural lipids and white butterfish oil
As can be seen from Table 8, when the mixture ratio is 10% -20% of palm kernel oil, 10% -20% of OPL structural fat, 60% -80% of freshwater white pomfret oil or the fractionated extract of freshwater white pomfret oil, the sn-2 palmitic acid in the obtained breast milk substitute fat accounts for 50% -60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 40% -50% of the total triglyceride content. The similarity score of the total fatty acids and the milk fat of Chinese mothers is more than 90 points, the similarity score of the triglyceride and the milk fat of Chinese mothers is more than 85 points, and the total similarity score is more than 85 points. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 14: composition for replacing fat with breast milk
The coconut oil, the palm oil, the OPL structural fat and the freshwater white pomfret oil prepared in the example 2 are selected as base oil, and similarity scores of total fatty acids, sn-2 site fatty acids and triglyceride in the obtained breast milk substitute fat composition and Chinese mother butterfat are shown in a table 9 under different proportions.
TABLE 9 scoring results for breast milk substitute fat composed of different coconut oil, palm oil, OPL structure fat and white pomfret oil
As can be seen from Table 9, when the mixture ratio is 10% -20% of coconut oil, 1% -20% of palm oil, 1% -20% of OPL structural fat, 60% -80% of freshwater white pomfret oil or freshwater white pomfret oil extract, the sn-2 palmitic acid in the obtained breast milk substituted fat accounts for 50% -60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 40% -50% of the total triglyceride content. The total similarity score is above 80 points. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 15: composition for replacing fat with breast milk
Selecting palm kernel oil, soybean oil, peanut oil, palm oil and the freshwater white pomfret oil extracted in example 2 as base oil, wherein the ratio of the palm kernel oil to the freshwater white pomfret oil is as follows: soybean oil: peanut oil: palm oil: fresh water white pomfret oil 19.16%: 14.54%: 2.34%: 2.10%: 61.85% with a palmitic acid content of 25.50% in the fatty acid composition at the sn-2position, calculated as 41.89% of the total palmitic acid content. The sum of OPO and OPL accounted for 31.99% of the total triglyceride content. The similarity scores of the total fatty acids, the sn-2position fatty acids and the triglyceride with the milk fat of Chinese mothers are 93.04, 68.23 and 87.71 respectively, and the total similarity score is 82.09. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 16: composition for replacing fat with breast milk
Selecting palm kernel oil, palm oil, MLCT structure fat, OPL structure fat and freshwater white pomfret oil extracted in example 2 as base oil, wherein the ratio of the palm kernel oil to the freshwater white pomfret oil is as follows: palm oil: MLCT structural lipid: OPL structural lipid: 10% of freshwater white pomfret oil: 5%: 10%: 15%: 60% with a palmitic acid content of 41.30% in the fatty acid composition at the sn-2position, calculated as 54.46% of the total palmitic acid content. The sum of OPO and OPL accounted for 38.21% of the total triglyceride content. The similarity scores of the total fatty acids, the sn-2position fatty acids and the triglyceride with the milk fat of Chinese mothers are 93.47, 92.45 and 95.45 respectively, and the total similarity score is 93.79. The average similarity score of total fatty acids, sn-2 fatty acids and triglycerides with the milk fat of Chinese mothers was 56.12, 33.51 and 17.51, and the total similarity score was 35.72 for 180 commercial infant formula oils measured by Sungclent. Therefore, compared with the oil for infant formula milk powder, the total similarity of the breast milk substitute fat and Chinese human mother milk fat is obviously improved, and the similarity of the breast milk substitute fat on the sn-2 fatty acid composition and the triglyceride composition is also obviously improved.
Example 17: infant formula milk powder
An infant formula comprising the breast milk substitute fat composition of example 12. The composition comprises (by weight portion): 400 parts of skim milk, 300 parts of desalted whey powder, 100 parts of breast milk substitute fat composition, 5 parts of phospholipid, 2 parts of DHA powder, 2 parts of arachidonic acid powder, 50 parts of white granulated sugar, 50 parts of lactose, 20 parts of concentrated whey protein powder, 10 parts of galacto-oligosaccharide, 20 parts of fructo-oligosaccharide, 1 part of sialic acid, 1 part of nucleotide, 5 parts of choline, 0.2 part of lutein, 2 parts of compound vitamin and 2 parts of compound mineral substance.
Example 18: formula milk powder for old people
An aged formula containing the breast milk-substitute fat composition of example 16. The composition comprises (by weight portion): 500 parts of skimmed milk powder, 200 parts of whey protein powder, 50 parts of breast milk substitute fat composition, 20 parts of marine fish bone collagen oligopeptide powder, 10 parts of galacto-oligosaccharide, 10 parts of fructo-oligosaccharide, 2 parts of compound vitamin and 2 parts of compound mineral.
Comparative example 1: other fish oils
The following fish oils were extracted by the chloroform methanol method and the OPL and OPO contents were measured, and the results are shown in Table 10. The golden pomfret oil and the freshwater white pomfret oil contain ultra-high content of OPL and OPO, the content of OPL in the special golden pomfret oil is higher than that of OPO, the golden pomfret oil is very similar to milk fat in Chinese mothers, the golden pomfret oil is more suitable for being used in substitutes of Chinese breast milk, and natural breast milk substitute fat can be prepared without adding OPO or OPL structure fat additionally and can be used in milk powder products.
TABLE 10 OPL and OPO content (%)
Comparative example 2: composition for replacing fat with breast milk
Coconut oil, soybean oil, palm oil and Bass sand fish oil are selected as base oil, and the mixture ratio is coconut oil: soybean oil: palm oil: basha fish oil 18.11%: 20.67%: 8.63%: 52.58% in which the fatty acid composition at the sn-2position has palmitic, oleic and linoleic acid contents of 28.72%, 27.42% and 22.16%, respectively, and 48.33% of the palmitic acid in total palm is calculated to be distributed at the sn-2 position. The total content of OPO and OPL was 36.75%. The similarity scores of the total fatty acids, sn-2 fatty acid and triglyceride and the Chinese mother milk fat are 95.61, 57.06 and 74.37 respectively, and the total similarity score is 75.68. It can be seen that the similarity scores of the sn-2position fatty acid and triglyceride and Chinese mother milk fat are lower compared with the prepared breast milk substitute fat, and are not beneficial to the digestion and absorption of infants.
Comparative example 3: commercial infant formula milk powder
Weighing 1 g of commercial cow milk/vegetable oil-based infant formula milk powder, adding 10mL of 65 ℃ hot water, fully mixing and cooling. Adding 2.0mL of ammonia water, fully mixing, putting into a water bath kettle at about 65 ℃, heating for 15-20 min, taking out from time to time, and oscillating. After removal, the mixture was cooled to room temperature, 10mL of ethanol was added, and the mixture was gently but thoroughly mixed. Adding 25mL of diethyl ether and petroleum ether respectively for extraction, taking the upper organic phase after layering, and repeating for 2-3 times. All organic phases were combined, the organic solvent was removed by rotary evaporation at 40 ℃ and the resulting fat was stored in a freezer at-20 ℃ until use. The commercial infant formula was found to have a palmitic acid, oleic acid, linoleic acid content of 20.03%, 31.63% and 20.44% and a sn-2 palmitic acid content of 33.94% based on total fatty acids. The OPO content was 10.31%, and OPL was not detected. The total fatty acids, sn-2 fatty acids and triglycerides were evaluated separately and scored as 85.78, 44.89 and 39.72, with an average score of 56.80, with more losses in sn-2 fatty acids and triglycerides than the infant formula in example 13.
The compositions of fatty acid, sn-2 site fatty acid and triglyceride of the Chinese mother milk fat are shown in tables 11-12, and data are derived from the composition, similarity evaluation and preparation research of Sunwong breast milk substitute fat [ D ] [ Boshi academic thesis ]. Wuxi: Jiangnan university, 2018.
Fatty acid and sn-2 site fatty acid composition of milk fat of Chinese mother butterfat in Table 11 (%)
Note: "-" indicates no concern.
TABLE 12 triglyceride composition of Chinese mother butterfat (%)
Tables 13-15 compositions of fatty acids, sn-2 fatty acids and triglycerides of natural and modified oils involved in the physical blending process.
TABLE 13 Total fatty acid composition (%)
Note: ND means not detected.
TABLE 14 fatty acid composition at sn-2 site of Natural oils and fats and modified oils (%)
TABLE 15 triglyceride composition of natural oils and fats and modified oils (%)
Note: ND means not detected.
Lipid metabolism was measured for the breast milk substitute fat and palm oil formulated in example 7 (coconut oil: soybean oil: palm oil: sanda fish oil: golden pomfret oil: 18.16%: 0.11%: 0.06%: 25.53%: 56.14%) and example 12 (palm kernel oil: soybean oil: palm oil: MLCT structural fat: golden pomfret oil: 10%: 5%: 5%: 15%: 65%), as follows:
laboratory animal cleaning grade Wistar rats (1 week old, male) were housed in the center of cleaning grade laboratory animals at a temperature (23+2 ℃), a humidity of 60%, free access to water and food. The care and pretreatment of the experimental animals are carried out according to the relevant regulations of the regulations on the management of experimental animals. After 28 male Wistar rats were prepared and fed with basal diet for one week, they were randomly divided into 4 groups, experimental group 1, experimental group 2, experimental group 3 and basal control group. After one week of adaptation, the animals are fed according to the feed formula. The feed formulations of the groups were prepared according to the feed formulation of the experimental animals recommended by the American society for Nutrition, and the fats and oils in the experimental group 1, the experimental group 2, the experimental group 3 and the basic control group were the same amount of the human milk substitute fat and the palm oil prepared in example 7, example 12 and comparative example 2, respectively. The fat sources of the feeds are different, and the energy, various main nutrients, mineral substances and various trace elements are consistent, which is shown in table 16. Bedding and drinking water were changed daily, pups were fed individually in metabolic cages, feeding experiments were performed for 2 weeks, and average food intake and weight gain were recorded.
TABLE 16 basal diet formulation for young mice
The feeding experiment was carried out for two weeks, and the feces were collected for the last 3 days in time, respectively. At the end of each day, the collected feces were weighed and stored frozen at-20 ℃. After the experimental period is finished, all the manure samples in the cages are respectively mixed and uniformly mixed, a part of the mixture is frozen and dried, crushed and sieved by a 40-mesh sieve, and the mixture is frozen and stored at the temperature of minus 20 ℃. The lipid and mineral composition of the mouse feces was determined and the results are shown in tables 17 and 18.
TABLE 17 dietary intake and growth status of mice
During the two-week feeding experiment, there was no significant difference in dietary intake and fecal output between the groups, and the body weights of experiment group 1 and experiment group 2 had a certain tendency to increase compared to the other two groups. The color of feces was darker and the water content was lower in the experimental group 3 and the basal control group than in the other two groups.
Table 18 apparent absorption of fat and minerals in mice
Note: apparent absorption rate (intake-content in feces)/intake × 100%
The results of the lipid and mineral composition of mouse feces are shown in Table 18. Total fat refers to the sum of triacylglycerols and free fatty acids in feces, and experimental group 1 was not significantly different from experimental group 2, and had a lower fat excretion amount than experimental group 3 and the basal control group. It can be seen that the breast milk-substituted lipids in examples 7 and 12 are more favorable for fat absorption.
The percentage of fecal calcium in experimental group 1 and experimental group 2 was significantly lower than that in experimental group 3 and the basal control group, and the apparent absorption rate of calcium was negatively correlated therewith. There was no significant difference in the apparent absorption of magnesium. This is probably because palmitic acid is easily combined with calcium at the sn-1,3 position to form calcium soap, which has low solubility in bile and is hardly absorbed by fat and calcium in the soap. It can be concluded that the breast milk substitute lipids prepared in examples 7 and 12 reduce calcium excretion in feces and improve calcium absorption.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (30)
1. The application of pomfret oil or its fraction in preparing milk substitute fat or milk product is characterized by that said pomfret oil includes one or two of golden pomfret oil and fresh water white pomfret oil.
2. The use of claim 1, wherein the golden pompano fish oil comprises trachinotus ovatus oil or trachinotus blosskii oil.
3. The use of claim 1, wherein the pomfret oil is extracted by a solvent method or an aqueous enzymatic method; the pomfret oil fractionation matter is obtained by fractionation of pomfret oil by adopting a dry fractionation method or a solvent fractionation method.
4. The breast milk fat-replacing composition is characterized in that the composition is prepared by taking pomfret oil or pomfret oil extract as base oil and adding other natural oil and/or modified oil, wherein sn-2-position palmitic acid accounts for 38-60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 23-50% of the total triglyceride content in percentage by mass; wherein the pomfret oil comprises one or two of golden pomfret oil and freshwater white pomfret oil.
5. The fat-substitute composition for human milk according to claim 4, wherein the palmitic acid in sn-2position accounts for 50 to 60% of the total palmitic acid content, and the sum of OPO and OPL accounts for 35 to 50% of the total triglyceride content.
6. The breast milk-substitute fat composition of claim 4, wherein the natural fat comprises one or more of soybean oil, peanut oil, palm oil, coconut oil, palm kernel oil, and basha fish oil.
7. The human milk substitute fat composition of claim 4, wherein the modified fat comprises one or more of OPO-structured fat, OPL-structured fat, and MLCT-structured fat.
8. The breast milk fat-substitute composition according to any one of claims 4 to 7, comprising, in mass percent: 1-50% of natural oil and/or modified oil and 50-99% of pomfret oil or pomfret oil extract.
9. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 1-50% of natural oil and 50-99% of pomfret oil or pomfret oil extract.
10. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 1-40% of coconut oil or/and palm kernel oil and 60-99% of golden pompano oil or golden pompano oil fractionation substance.
11. The human milk-substitute fat composition according to claim 10, comprising, in mass percent: 10-20% of coconut oil or/and palm kernel oil and 80-90% of golden pompano oil or golden pompano oil fractionation substance.
12. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 10 to 20 percent of coconut oil or/and palm kernel oil, 1 to 20 percent of palm oil and 60 to 89 percent of golden pompano oil or golden pompano oil fraction.
13. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 10-20% of coconut oil or/and palm kernel oil, 0-10% of soybean oil, 0-10% of peanut oil, 0-10% of palm oil, 20-30% of sandfish oil and 50-70% of golden pompano oil or golden pompano oil extract.
14. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 0-10% of soybean oil, 0-10% of peanut oil, 0-10% of palm oil, 10-20% of coconut oil, 40-50% of sandwort fish oil and 30-45% of golden pompano oil or golden pompano oil extract.
15. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 10-20% of coconut oil or/and palm kernel oil, 10-20% of soybean oil, 0-5% of peanut oil, 0-5% of palm oil and 60-80% of freshwater white pomfret oil or freshwater white pomfret oil extract.
16. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 1-40% of natural oil, 1-20% of modified oil and 50-98% of pomfret oil or pomfret oil extract.
17. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 10 to 20 percent of coconut oil or/and palm kernel oil, 1 to 20 percent of OPO structure fat and 60 to 89 percent of golden pompano oil or golden pompano oil fraction.
18. The human milk-substitute fat composition according to claim 17, comprising, in mass percent: 10 to 20 percent of coconut oil or/and palm kernel oil, 1 to 5 percent of OPO structure fat and 75 to 89 percent of golden pompano oil or golden pompano oil fraction.
19. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 10 to 20 percent of coconut oil or/and palm kernel oil, 1 to 20 percent of soybean oil, 1 to 20 percent of OPO structure fat and 60 to 80 percent of golden pompano oil or golden pompano oil extract.
20. The human milk-substitute fat composition according to claim 19, comprising, in mass percent: 10 to 20 percent of coconut oil or/and palm kernel oil, 1 to 5 percent of soybean oil, 1 to 5 percent of OPO structure fat and 70 to 88 percent of golden pompano oil or golden pompano oil extract.
21. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: soybean oil 1-20 wt%, palm oil 1-20 wt%, MLCT structure fat 1-20 wt% and golden pomfret oil 60-80 wt% or golden pomfret oil fraction.
22. The human milk-substitute fat composition according to claim 21, comprising, in mass percent: soybean oil 1-5 wt%, palm oil 1-10 wt%, MLCT structure fat 10-20 wt% and golden pomfret oil 65-88 wt% or golden pomfret oil fraction.
23. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 1 to 40 percent of natural grease, 1 to 30 percent of modified grease and 50 to 98 percent of freshwater white pomfret oil or the fractionated matters of the freshwater white pomfret oil.
24. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 10-20% of coconut oil or/and palm kernel oil, 1-20% of OPL structure fat, 60-89% of freshwater white pomfret oil or freshwater white pomfret oil fractionation substance.
25. The human milk-substitute fat composition according to claim 24, comprising, in mass percent: 10-20% of coconut oil or/and palm kernel oil, 10-20% of OPL structure fat and 60-80% of freshwater white pomfret oil or freshwater white pomfret oil fractionation substance.
26. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 10 to 20 percent of coconut oil or/and palm kernel oil, 1 to 20 percent of palm oil, 1 to 20 percent of OPL structure fat and 60 to 80 percent of freshwater white pomfret oil or freshwater white pomfret oil fractionation substance.
27. The human milk-substitute fat composition according to claim 26, comprising, in mass percent: 10-20% of coconut oil or/and palm kernel oil, 1-5% of palm oil, 10-20% of OPL structure fat and 55-79% of freshwater white pomfret oil or freshwater white pomfret oil extract.
28. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 0-15% of coconut oil or/and palm kernel oil, 0-5% of soybean oil, 0-5% of peanut oil, 0-5% of palm oil, 0-10% of OPL structural fat, 10-20% of MLCT structural fat and 50-80% of freshwater white pomfret oil or freshwater white pomfret oil extract.
29. The human milk fat-substitute composition according to any one of claims 4 to 7, wherein the human milk fat-substitute composition comprises, in mass percent: 0-10% of soybean oil, 0-10% of peanut oil, 0-10% of palm oil, 10-20% of coconut oil, 15-25% of basha fish oil, 20-30% of OPL structure fat and 25-40% of freshwater white pomfret oil or a freshwater white pomfret oil extract.
30. A food product comprising the breast milk fat-substitute composition of any one of claims 4 to 29.
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| CN111700125A (en) | 2020-09-25 |
| CN112369472A (en) | 2021-02-19 |
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