WO2022166766A1 - 一种用于固相萃取及芯片阵列点样的液体工作站 - Google Patents

一种用于固相萃取及芯片阵列点样的液体工作站 Download PDF

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WO2022166766A1
WO2022166766A1 PCT/CN2022/074360 CN2022074360W WO2022166766A1 WO 2022166766 A1 WO2022166766 A1 WO 2022166766A1 CN 2022074360 W CN2022074360 W CN 2022074360W WO 2022166766 A1 WO2022166766 A1 WO 2022166766A1
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module
positive pressure
phase extraction
nitrogen blowing
plate
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PCT/CN2022/074360
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English (en)
French (fr)
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梁洞泉
张其峰
梁松伟
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药明激创(佛山)生物科技有限公司
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Publication of WO2022166766A1 publication Critical patent/WO2022166766A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86

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  • the invention relates to the field of biology, in particular to a liquid workstation for solid phase extraction and chip array spotting.
  • biomarkers have been proposed.
  • a class of markers related to cell growth and proliferation can not only explore the pathogenesis at the molecular level, but also have unique advantages in accurately and sensitively evaluating the early and low-level damage of the body, providing early warning of diseases, which is largely responsible for Clinicians provide the basis for auxiliary diagnosis.
  • Biomarkers are closely related to diseases and have great prospects in diagnosis. Compared with current clinical detection methods, the enrichment of disease-related biomarkers in the human body for diagnosis has the advantages of simple, convenient and painless detection.
  • biomarkers for research and diagnosis is a complex and tedious process, which includes extraction, purification, drying, spotting, liquid chromatography and flight mass spectrometry.
  • extraction, purification, drying, spotting, liquid chromatography and flight mass spectrometry When the number of samples continues to increase, multiple laboratory workers are required to consume a lot of time to complete the entire process, and researchers are exposed to chemicals for a long time, which is harmful to health.
  • the traditional manual operation can no longer meet the high-throughput and fast processing, and the full automation or partial automation of the experimental process is the development trend of this application.
  • the purpose of the present invention is to provide a liquid workstation for solid phase extraction and chip array spotting, so as to solve the problems raised in the above background art.
  • the present invention provides the following technical solutions:
  • a liquid workstation for solid-phase extraction and chip array spotting comprising an L-shaped working platform, a plurality of working disk positions arranged on the horizontal plane of the working platform, and a disk shifting claw for grasping the working disk positions.
  • the bay includes a positive pressure module, a nitrogen blowing module, a tip placement module, a product collection module, a sampling module, a reagent storage module, a solid phase extraction module, an oscillation temperature control module and a sample module, among which:
  • the positive pressure module includes a positive pressure head main part and a positive pressure head seat, which is used to pressurize the SPE column.
  • the lower part of the positive pressure head main part is fixedly connected with the positive pressure head seat, and the upper and lower ends of the positive pressure head seat are fixedly connected.
  • Open and hollow inside; an elbow joint is fixedly installed on the upper part of the main part of the positive pressure head, one end of the elbow joint is connected with the pipeline, the other end of the elbow joint is connected with the main part of the positive pressure head, and the pipeline is compressed with air
  • a positive pressure sealing ring and a sealing ring fixing plate are installed between the positive pressure head main part and the positive pressure head seat, and a plurality of through holes for gas flow are opened on the sealing ring fixing plate;
  • the nitrogen blowing module includes a nitrogen blowing seat and a number of nitrogen blowing needles fixedly installed at the bottom of the nitrogen blowing seat.
  • the nitrogen blowing seat is hollow inside, the nitrogen blowing needle is communicated with the nitrogen blowing seat, and a sealing cover is installed on the top of the nitrogen blowing seat.
  • a nitrogen quick connector is connected to the side of the blowing seat;
  • a temperature control adapter is arranged below the product collection module, and a collection tube rack is arranged above.
  • the collection tube rack is not limited to test tubes and centrifuge tubes, and the SPE column and the nitrogen blowing module can be stacked on the collection tube rack respectively;
  • the collection pipe rack includes an SPE column rack, and the SPE column rack is provided with an SPE collection pipe for storing the SPE column;
  • the spotting module includes a vial adapter set on the left side and a chip adapter set on the right side for chip fixing, and a temperature control adapter is arranged at the bottom of the chip adapter;
  • the reagent storage module includes a thermal insulation shell and a semiconductor refrigerating sheet arranged at the bottom of the thermal insulation shell, a cold conduction plate is arranged between the thermal insulation shell and the semiconductor refrigerating sheet, and an upper refrigeration adapter for storing a plurality of containers is installed on the upper part of the thermal insulation shell ;
  • the solid oscillation temperature control module includes an oscillator upper plate, an eccentric shaft and an oscillating motor.
  • a host connecting plate is fixedly installed below the oscillator upper plate, and the oscillating motor is fixedly installed on the bottom of the host connecting plate through an oscillating motor seat. The output end of the oscillating motor is fixedly connected with the eccentric shaft.
  • two pin fixing plates I are symmetrically installed on the left and right sides of the upper part of the positive pressure head main part, and several pins for fixing the positive pressure head main part are fixedly installed on the pin fixing plate I. I.
  • the nitrogen quick connector is communicated with the nitrogen bottle, and the number of the nitrogen blowing needles is 12.
  • a nitrogen blowing sealing ring is arranged at the connection between the nitrogen blowing seat and the sealing cover.
  • a fan bracket is arranged below the semiconductor refrigeration sheet, a fan is installed on the fan bracket, and a copper radiator and a heat conduction plate are also arranged between the semiconductor refrigeration sheet and the fan.
  • the upper plate of the oscillator is connected and installed with a collection tube adapter, on which is placed a number of collection tubes that are driven to oscillate by the vibration shaft; board connection.
  • connection between the output end of the oscillating motor and the eccentric shaft is further provided with a balance block and a return spring adapted to the eccentric shaft.
  • the sample module includes a lower plate of the sample rack and an upper plate of the sample rack, and the lower plate of the sample rack and the upper plate of the sample rack are connected and fixed by a pillar.
  • positive pressure module, nitrogen blowing module, tip placement module, product collection module, sampling module, reagent storage module, solid phase extraction module, oscillation temperature control module and sample module are discharged in a nine-square grid.
  • the vertical surface of the working platform is provided with a mechanical arm assembly for driving the plate-moving claw to adjust the position
  • the mechanical arm assembly includes an x-axis mechanical arm that drives the plate-moving claw to move along the x-axis, and a drive plate-moving claw.
  • the y-axis robotic arm that moves the gripper along the y-axis and the z-axis robotic arm that drives the shifting gripper to move along the z-axis.
  • the beneficial effects of the present invention are: the present invention can be used in the fields of clinical examination, scientific research, blood bank and the like, and the specific biomarkers of the human body are used as samples, including but not limited to samples such as saliva, serum, and urine. Rapid biomarker extraction, purification, and automatic product drying and spotting are carried out in the liquid workstation, and the product processed by the liquid workstation of the present invention can be used for liquid chromatography and flight mass spectrometry detection.
  • the solid-phase extraction of biomarkers, product nitrogen drying and spotting platform of the present invention can realize the automation and convenience of the experiment, reduce the work intensity of the inspectors, greatly reduce the misoperation of the inspectors, and facilitate the Same management of data, etc.
  • the workstation has a wide range of uses and can be used in scientific research, medical and other fields.
  • Figure 1 is a schematic diagram of the structure of a liquid workstation used for solid phase extraction and chip array spotting.
  • FIG. 2 is a schematic structural diagram of a working platform in a liquid workstation for solid-phase extraction and chip array spotting.
  • FIG. 3 is a schematic diagram of the structure of the plate transfer claw in the liquid workstation used for solid phase extraction and chip array spotting.
  • FIG. 4 is a schematic structural diagram of a positive pressure module in a liquid workstation used for solid phase extraction and chip array spotting.
  • FIG. 5 is a schematic structural diagram of a nitrogen blowing module in a liquid workstation used for solid phase extraction and chip array spotting.
  • FIG. 6 is a schematic structural diagram of a collection tube rack in a liquid workstation used for solid phase extraction and chip array spotting.
  • FIG. 7 is a schematic structural diagram of a reagent storage module in a liquid workstation used for solid phase extraction and chip array spotting.
  • FIG. 8 is a schematic structural diagram of a solid-phase extraction module in a liquid workstation used for solid-phase extraction and chip array spotting.
  • FIG. 9 is a schematic structural diagram of a sample module in a liquid workstation used for solid phase extraction and chip array spotting.
  • a liquid workstation for solid-phase extraction and chip array spotting includes an L-shaped working platform 1 , a plurality of working disks disposed on the horizontal surface of the working platform 1 , and The plate-moving claw 3 that grabs the working disk, which includes a positive pressure module 4, a nitrogen blowing module 5, a gun tip placement module 6, a product collection module 7, a sample spotting module 8, and a reagent storage module 9 , solid phase extraction module 10, oscillation temperature control module 11 and sample module 12, wherein:
  • the positive pressure module 4 includes a positive pressure head main part 41 and a positive pressure head seat 47 for pressurizing the SPE column.
  • the lower part of the positive pressure head main part 41 is fixedly connected with the positive pressure head seat 47, and the positive pressure
  • the upper and lower ends of the head seat 47 are open and the interior is hollow; the upper part of the positive pressure head main part 41 is fixedly installed with an elbow joint 44, one end of the elbow joint 44 is communicated with the pipeline, and the other end of the elbow joint 44 is connected with the positive pressure head.
  • the main part 41 is in communication, and the pipeline is in communication with the air compressor; a positive pressure sealing ring 45 and a sealing ring fixing plate 46 are installed between the positive pressure head main part 41 and the positive pressure head seat 47, and the sealing ring fixing plate 46 is installed on the A number of through holes for gas flow are provided, and the air compressor is ventilated into the pipeline.
  • the gas is fed into the positive pressure head main part 41 and the positive pressure head seat 47 along the pipeline and the elbow joint 44 to pressurize the SPE column.
  • the SPE column is a solid-phase extraction column, which is a sample pretreatment device developed from a chromatography column for extraction, separation and concentration.
  • the elbow joint 44 is provided with a three-way valve, the positive pressure head is closely attached to the SPE column, the air compressor generates power, and the three-way valve adjusts and controls the speed of the liquid outflow of the solid phase extraction column;
  • the number of positive pressure head holes of the positive pressure module 4 is designed according to the number of samples, and preferably, the number of positive pressure head holes is four;
  • two pin fixing plates I are symmetrically installed on the left and right sides of the upper part of the positive pressure head main part 41, and several pin fixing plates I are fixedly installed on the positive pressure head main parts for aligning the positive pressure head main parts.
  • 41 Pin I42 for fixing is symmetrically installed on the left and right sides of the upper part of the positive pressure head main part 41, and several pin fixing plates I are fixedly installed on the positive pressure head main parts for aligning the positive pressure head main parts.
  • the nitrogen blowing module 5 includes a nitrogen blowing seat 51 and a number of nitrogen blowing needles 52 fixedly installed at the bottom of the nitrogen blowing seat 51.
  • the nitrogen blowing seat 51 is hollow inside, the nitrogen blowing needle 52 is communicated with the nitrogen blowing seat 51, and the nitrogen blowing seat is A sealing cover 55 is installed on the top of the 51, and a nitrogen quick connector 54 is connected to the side of the nitrogen blowing seat 51.
  • the nitrogen quick connector 54 is ventilated into the nitrogen blowing seat 51, and the gas is released by the nitrogen blowing needle 52, and the sample is dried;
  • the nitrogen quick connector 54 is communicated with the nitrogen bottle, and the number of the nitrogen blowing needles 52 is set according to the number of samples.
  • the nitrogen blowing module 5 has 12 channels.
  • the number of blowing needles 52 is 12;
  • a nitrogen blowing sealing ring 53 is provided at the connection between the nitrogen blowing seat 51 and the sealing cover 55 , so as to improve the sealing performance of the nitrogen blowing seat 51 .
  • the pipette tip placement module 6 is provided with a pipette tip adapter slot, which can place 1000, 200, 100 and 50 ⁇ L pipette tips according to different experiments.
  • the pipette tip placement module 6 is configured with 1000 ⁇ L and 50 ⁇ L pipette tip adapter grooves.
  • a temperature control adapter is arranged below the product collection module 7 to accelerate nitrogen drying, and a collection tube rack 15 is arranged above.
  • the collection tube rack 15 is not limited to test tubes and centrifuge tubes, and the collection tube racks 15 can be stacked on top of each other.
  • SPE column and nitrogen blowing module 5 are used for receiving and drying the purified product respectively;
  • the temperature control adapter is set to a temperature of 80°C;
  • the collection tube rack 15 includes an SPE column rack 1501 , and the SPE column rack 1501 is provided with an SPE collection tube 1503 for storing the SPE column 1502 .
  • the spotting module 8 includes a vial adapter set on the left and a chip adapter set on the right for chip fixing, and the chip adapter has a temperature control adapter at the bottom for quick drying after spotting;
  • 12 liquid phase vials are placed in the vial adapter, a MADLI chip is fixed in the chip adapter, and the temperature control adapter is set to a temperature of 80°C.
  • the reagent storage module 9 includes a thermal insulation shell 909 and a semiconductor refrigeration chip 905 arranged at the bottom of the thermal insulation shell 909.
  • a cold conduction plate 908 is arranged between the thermal insulation shell 909 and the semiconductor refrigeration chip 905.
  • a temperature sensor 911 for temperature measurement is also installed on the thermal insulation shell 909;
  • a fan bracket 902 is arranged below the semiconductor cooling sheet 905, a fan 901 is mounted on the fan bracket 902, and a copper radiator 903 and a heat conduction device are also arranged between the semiconductor cooling sheet 905 and the fan 901.
  • the plate 904 and the semiconductor cooling sheet 905 conduct heat during the cooling process to the heat conduction plate 904 , and the heat is dissipated by the copper radiator 903 and the fan 901 .
  • the solid oscillation temperature control module 11 includes an oscillator upper plate 1010, an eccentric shaft 1005 and an oscillating motor 1001.
  • a host connecting plate 1007 is fixedly installed under the oscillator upper plate 1010, and the oscillating motor 1001 passes through the oscillating motor base 1002. It is fixedly installed at the bottom of the connecting plate 1007 of the main engine, and the output end of the oscillation motor 1001 is fixedly connected with the eccentric shaft 1005;
  • a collection tube adapter 1012 is connected and installed on the upper plate 1010 of the oscillator, and a plurality of collection tubes 1014 oscillated by the vibration shaft 1005 are placed on the collection tube adapter 1012; the collection tube adapter 1012 is fixed by pins
  • the board II 1011 is connected to the upper board 1010 of the oscillator;
  • connection between the output end of the oscillating motor 1001 and the eccentric shaft 1005 is further provided with a balance block 1006 and a return spring 1004 adapted to the eccentric shaft 1005;
  • a certain number of collection tubes 1014 are fixed by the collection tube adapter 1012 for the reaction of the sample and various solutions.
  • the solid oscillation temperature control module 11 provides temperature and vibration for the reaction of the sample, realizes the rapid rise and fall of the temperature in the experimental process, and shortens the reaction time.
  • the collection tube adapter 1012 fixes 12 2mL collection tubes 1014. In the experiment, the sample is first heated to 65°C and shaken to incubate the sample to denature the protein at high temperature and loosen the molecular structure.
  • the sample module 12 includes a sample holder lower plate 1201 and a sample holder upper plate 1203 .
  • the sample holder lower plate 1201 and the sample holder upper plate 1203 are connected and fixed by a support 1202 for placing sample test tubes 1204 .
  • the components on the working disk include a positive pressure module 4, a nitrogen blowing module 5, a tip placement module 6, a product collection module 7, a sample spotting module 8, and a reagent storage module.
  • the spotting module 8, the reagent storage module 9, the solid phase extraction module 10, the oscillation temperature control module 11 and the sample module 12 are arranged in a nine-square grid, so that the working area is relatively independent and separated, avoiding the sample area, the reagent area and the solid phase extraction area, etc. The areas are polluted with each other, and the space of the working base can be maximized.
  • the vertical surface of the working platform 1 is provided with a mechanical arm assembly for driving the moving plate claw 3 to adjust the position
  • the mechanical arm assembly includes an x-axis mechanical mechanism that drives the moving plate claw 3 to move along the x-axis.
  • a method for using a liquid workstation for solid phase extraction and chip array spotting comprising the following steps:
  • Pipette tip placement module places 1000ul and 50ul pipette tips; product collection module places 12 2mL centrifuge tubes, solid phase extraction module places 12 1mL SPE columns; spotting module places 12 liquid phase vials and MADLI chips respectively;
  • the oscillating temperature control module 11 is placed with 12 2mL centrifuge tubes; the sample module 12 is placed with 12 blood collection tubes containing the serum to be processed;
  • the robotic arm assembly takes 50 ⁇ L of the pipette tip from the pipette tip placement module to the blood collection tube, and 30 ⁇ L of supernatant serum is added to the centrifuge tube on the shaking temperature control module.
  • the robotic arm assembly takes 50 ⁇ L of the pipette tip from the pipette tip placement module to the reagent storage module, and adds 30 ⁇ L of reagent A to the centrifuge tube in the centrifuge tube adapter.
  • the temperature is raised to 65°C, and after 1500 rmp for 5 minutes, the temperature drops to 45°C.
  • Phase extraction module Phase extraction module; add 1mL E reagent from the reagent injector to the SPE column in the solid phase extraction rack; the robotic arm assembly takes the positive pressure head from the positive pressure module to the solid phase extraction column rack to pressurize the column; The pressure head is moved back to the positive pressure module; the reagent injector is added to the SPE solid phase extraction rack and 1 mL of G reagent is added; the robotic arm assembly takes the positive pressure head from the positive pressure module to the SPE solid phase extraction rack to pressurize the column; Move the pressure head back to the positive pressure module; add 1mL E reagent from the reagent injector to the SPE extraction column holder; the robotic arm assembly takes the positive pressure head from the positive pressure module to the SPE extraction column holder to pressurize the column; the robotic arm assembly puts the positive pressure head Move back to the positive pressure module; the robotic arm assembly takes 50 ⁇ L of the pipette tip from the pipette tip placement module to the reagent storage module and takes 5 ⁇ L of the C
  • the robotic arm assembly moves the positive pressure head back to the positive pressure module; the reagent injector 1 adds 1 mL of reagent to the SPE extraction column holder, and the robotic arm assembly takes the positive pressure head from the positive pressure module to the SPE extraction column holder to pressurize the column.
  • the assembly moves the positive pressure head back to the positive pressure module, and repeats this step twice; the robotic arm assembly stacks the SPE extraction column rack on the product collection rack; reagent injector 3 adds 1 mL of G reagent to the SPE extraction column rack; robotic arm The assembly takes the positive pressure head from the positive pressure module to the SPE extraction column rack and pressurizes the column; the robotic arm assembly moves the positive pressure head back to the positive pressure module; the robotic arm assembly moves the SPE extraction rack on the product collection adapter to the solid phase extraction column module ; The mechanical claw moves the nitrogen blowing head to the top of the centrifuge tube adapter, blowing nitrogen at 80°C, and the nitrogen blowing head descends at regular intervals until the product is blown dry; the robotic arm assembly moves the nitrogen blowing head back to the nitrogen blowing module; the collection rack module
  • the temperature control assembly below is cooled to 25-37°C; the reagent injector is added to the centrifuge tube adapter to add 15 ⁇ LE reagent; the robotic arm assembly takes the
  • the liquid phase vial product taken out and the MADLI chip can be used for liquid phase and flight mass spectrometry assays to analyze sample components, which can be used for diagnosis.

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Abstract

一种用于固相萃取及芯片阵列点样的液体工作站，包括L型的工作平台（1）、若干设置于工作平台（1）水平面上的工作盘位和对工作盘位进行抓取的移盘爪（3），工作盘位包括有正压模块（4）、氮吹模块（5）、枪头放置模块（6）、产物收集模块（7）、点样模块（8）、试剂存储模块（9）、固相萃取模块（10）、振荡温控模块（11）和样品模块（12）。液体工作站可用于临床检验、科研、血站等领域，实现实验的自动化、便捷化、降低了检验人员的工作强度，以及减少了检验人员的误操作，并且便于数据的统一管理等。

Description

一种用于固相萃取及芯片阵列点样的液体工作站 技术领域
本发明涉及生物学领域，具体是一种用于固相萃取及芯片阵列点样的液体工作站。
背景技术
随着免疫学和分子生物学技术的发展，生物标记物这一个概念被提出。一类与细胞生长增殖有关的标志物，不仅可从分子水平探讨发病机制，而且在准确、敏感地评价机体早期、低水平的损害方面有着独特的优势，提供疾病早期预警，很大程度上为临床医生提供了辅助诊断的依据。生物标志物与疾病息息相关，在诊断作用有着巨大的前景，相较于现在临床使用的检测方法，从人体内富集疾病相关生物标记物进行诊断，具有检测简单、方便、无痛苦等优势。
利用生物标记物进行研究、诊断是一个复杂且繁琐的过程，其处理包括提取、纯化、干燥、点样、液相色谱和飞行质谱分析等流程。当样品数量不断的增加时，需要多名实验室员消耗大量时间来完成整个流程，且研究人员长期与化学品接触，有害健康。传统的手工操作已不能满足高通量、快速的处理，实验过程的全自动化或部分自动化是该应用发展的风向。
因此，针对以上现状，迫切需要开发一种用于固相萃取及芯片阵列点样的液体工作站，以克服当前实际应用中的不足。
发明内容
本发明的目的在于提供一种用于固相萃取及芯片阵列点样的液体工作站，以解决上述背景技术中提出的问题。
为实现上述目的，本发明提供如下技术方案：
一种用于固相萃取及芯片阵列点样的液体工作站，包括L型的工作平台、若干设置于工作平台水平面上的工作盘位和对工作盘位进行抓取的移盘爪，所述工作盘位包括有正压模块、氮吹模块、枪头放置模块、产物收集模块、点样模块、试剂存储模块、固相萃取模块、振荡温控模块和样品模块，其中：
所述正压模块包括正压头主件和正压头座，用于对SPE柱进行加压，所述正压头主件的下部与正压头座固定连接，正压头座上下两端开口、内部中空；所述正压头主件的上部固定安装有弯管接头，弯管接头的一端与管道连通、弯管接头的另一端与正压头主件连通，所述管道与空气压缩机连通；所述正压头主件与正压头座之间安装有正压密封圈和密封圈固定板，密封圈固定板上开设有若干供气体流动的通孔；
所述氮吹模块包括氮吹座和若干固定安装于氮吹座底部的氮吹针，所述氮吹座内部中空，氮吹针与氮吹座连通，氮吹座顶部安装有密封盖，氮吹座的侧边处连通有氮气快接头；
所述产物收集模块的下方设置有温控适配器，上方设置有收集管架，收集管架不限于试管和离心管，且收集管架上面可以分别叠放SPE柱和氮吹模块；
所述收集管架包括SPE柱架，SPE柱架中设置有用于存放SPE柱的SPE收集管；
所述点样模块包括左侧设置的小瓶适配器和右边设置的用于芯片固定的芯片适配器，所述芯片适配器底部有温控适配器；
所述试剂存储模块包括保温外壳和设置于保温外壳底部的半导体制冷片，保温外壳与半导体制冷片之间设置有冷传导板，所述保温外壳的上部安装有用于存放多个容器的上制冷适配器；
所述固振荡温控模块包括振荡器上板、偏心轴和振荡电机，所述振荡器上板的下方固定安装有主机连板，所述振荡电机通过振荡电机座固定安装于主机连板底部，振荡电机的输出端与偏心轴固定连接。
作为本发明进一步的方案：所述正压头主件上部的左右两侧对称安装有两块销钉固定板Ⅰ，销钉固定板Ⅰ上固定安装有若干用于对正压头主件进行固定的销钉Ⅰ。
作为本发明进一步的方案：所述氮气快接头与氮气瓶连通，所述氮吹针设置的数量为12个。
作为本发明进一步的方案：所述氮吹座与密封盖的连接处设置有氮吹密封圈。
作为本发明进一步的方案：所述半导体制冷片的下方设置有风扇支架，风扇支架上安装有风扇，半导体制冷片与风扇之间还设置有铜散热器和导热板。
作为本发明进一步的方案：所述振荡器上板连接安装有收集管适配器，收集管适配器上放置有若干由振动轴带动振荡的收集管；所述收集管适配器通过销钉固定板Ⅱ与振荡器上板连接。
作为本发明进一步的方案：所述振荡电机的输出端与偏心轴的连接处还设置有与偏心轴适配的平衡块和回位弹簧。
作为本发明进一步的方案：所述样品模块包括有样品架下板和样品架上板，样品架下板和样品架上板由支柱连接固定。
作为本发明进一步的方案：正压模块、氮吹模块、枪头放置模块、产物收集模块、点样模块、试剂存储模块、固相萃取模块、振荡温控模块和样品模块呈九宫格方式排放。
作为本发明进一步的方案：所述工作平台垂直面上设置有用于带动移盘爪进行位置调节的机械臂组件，机械臂组件包括驱动移盘爪沿x轴运动的x轴机械臂、驱动移盘爪沿y轴运动的y轴机械臂和驱动移盘爪沿z轴运动的z轴机器臂。
与现有技术相比，本发明的有益效果是：本发明可用于临床检验、科研、血站等领域，以人体特定的生物标记物作为样品，包括但不限于唾液、血清、尿液等样品中进行快速生物标记物提取、纯化、并自动完成产物干燥与点样，经由本发明的液体工作站处理后的产物可用于液相色谱和飞行质谱检测。此外，由于本发明的生物标记物固相萃取、产物氮吹干燥与点样平台能够实现实验的自动化、便捷化、降低了检验人员的工作强度，以及大地减少了检验人员的误操作，并且便于数据的同一管理等。该工作站有广泛的用途，可以用于科研、医疗等领域。
附图说明
图1为用于固相萃取及芯片阵列点样的液体工作站的结构示意图。
图2为用于固相萃取及芯片阵列点样的液体工作站中工作平台的结构示意图。
图3为用于固相萃取及芯片阵列点样的液体工作站中移盘爪的结构示意图。
图4为用于固相萃取及芯片阵列点样的液体工作站中正压模块的结构示意图。
图5为用于固相萃取及芯片阵列点样的液体工作站中氮吹模块的结构示意图。
图6为用于固相萃取及芯片阵列点样的液体工作站中收集管架的结构示意图。
图7为用于固相萃取及芯片阵列点样的液体工作站中试剂存储模块的结构示意图。
图8为用于固相萃取及芯片阵列点样的液体工作站中固相萃取模块的结构示意图。
图9为用于固相萃取及芯片阵列点样的液体工作站中样品模块的结构示意图。
图中：1-工作平台、101-急停按钮、102-调压阀、103-x轴机械臂、104-y轴机械臂、105-z轴机器臂、2-空气泵、3-移盘爪、31-外罩、32-旋转电机、33-旋转电机座、34-齿条、35-导轨、36-夹片、37-主机座、4-正压模块、41-正压头主件、42-销钉Ⅰ、43-销钉固定板Ⅰ、44-弯管接头、45-正压密封圈、46-密封圈固定板、47-正压头座、5-氮吹模块、51-氮吹座、52-氮吹针、53-氮吹密封圈、54-氮气快接头、55-密封盖、6-枪头放置模块、7-产物收集模块、8-点样模块、9-试剂存储模块、901-风扇、902-风扇支架、903-铜散热器、904-导热板、905-半导体制冷片、906-线束架、907-上制冷适配器、908-冷传导板、909-保温外壳、911、温度传感器、10-固相萃取模块、1001-振荡电机、1002-振荡电机座、1003-轴承座、1004-回位弹簧、1005-偏心轴、1006-平衡块、1007-主机连板、1008-隔热板、1009-加热板、1010-振荡器上板、1011-销钉固定板Ⅱ、1012-收集管适配器、1013-销钉Ⅱ、1014-收集管、11-振荡温控模块、12-样品模块、1201-样品架下板、1202-支柱、1203-样品架上板、1204-样品试管、13-废液位模块、14-枪头废弃槽、15-收集管架、1501-SPE柱架、1502-SPE柱、1503-SPE收集管。
具体实施方式
下面结合具体实施方式对本专利的技术方案作进一步详细地说明。
下面详细描述本专利的实施例，所述实施例的示例在附图中示出，其中自始至终相同或类似的标号表示相同或类似的元件或具有相同或类似功能的元件。下面通过参考附图描述的实施例是示例性的，仅用于解释本专利，而不能理解为对本专利的限制。
在本专利的描述中，需要理解的是，术语“中心”、“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”、“内”、“外”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本专利和简化描述，而 不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本专利的限制。
在本专利的描述中，需要说明的是，除非另有明确的规定和限定，术语“安装”、“相连”、“连接”、“设置”应做广义理解，例如，可以是固定相连、设置，也可以是可拆卸连接、设置，或一体地连接、设置。对于本领域的普通技术人员而言，可以根据具体情况理解上述术语在本专利中的具体含义。
请参阅图1-9，本发明实施例中，一种用于固相萃取及芯片阵列点样的液体工作站，包括L型的工作平台1、若干设置于工作平台1水平面上的工作盘位和对工作盘位进行抓取的移盘爪3，所述工作盘位包括有正压模块4、氮吹模块5、枪头放置模块6、产物收集模块7、点样模块8、试剂存储模块9、固相萃取模块10、振荡温控模块11和样品模块12，其中：
所述正压模块4包括正压头主件41和正压头座47，用于对SPE柱进行加压，所述正压头主件41的下部与正压头座47固定连接，正压头座47上下两端开口、内部中空；所述正压头主件41的上部固定安装有弯管接头44，弯管接头44的一端与管道连通、弯管接头44的另一端与正压头主件41连通，所述管道与空气压缩机连通；所述正压头主件41与正压头座47之间安装有正压密封圈45和密封圈固定板46，密封圈固定板46上开设有若干供气体流动的通孔，由空气压缩机向管道内部通气，气体沿管道、弯管接头44送入正压头主件41和正压头座47中，对SPE柱进行加压，用于SPE柱活化、上样、洗涤和洗脱的流速控制；
需要说明的是，在本发明实施例中，SPE柱为固相萃取柱，是从层析柱发展而来的一种用于萃取、分离、浓缩的样品前处理装置。
在本发明实施例中，所述弯管接头44上设有三通阀，通过正压头与SPE柱紧密贴合，空气压缩机产生动力，三通阀调节控制固相萃取柱液体流出的速度；
在本发明实施例中，需要说明的是，正压模块4的正压头孔道数目根据样品的数量设计，优选的，正压头孔道数目为四；
进一步的，在本发明实施例中，所述正压头主件41上部的左右两侧对称安装有两块销钉固定板Ⅰ，销钉固定板Ⅰ上固定安装有若干用于对正压头主件41进行固定的销钉Ⅰ42。
所述氮吹模块5包括氮吹座51和若干固定安装于氮吹座51底部的氮吹针52，所述氮吹座51内部中空，氮吹针52与氮吹座51连通，氮吹座51顶部安装有密封盖55，氮吹座51的侧边处连通有氮气快接头54，由氮气快接头54向氮吹座51内部通气，气体由氮吹针52释放，对样品进行干燥；
在本发明实施例中，所述氮气快接头54与氮气瓶连通，所述氮吹针52设置的数量根据样品的数量设置，优选的，氮吹模块5为12通道，可以理解的是，氮吹针52设置的数量为12个；
进一步的，在本发明实施例中，所述氮吹座51与密封盖55的连接处设置有氮吹密封圈53，用于提高氮吹座51的密封性。
所述枪头放置模块6中开设有枪头适配槽，可以根据实验的不同放置1000、200、100和50μL规格的枪头。优选的，根据整个实验所需，枪头放置模块6配置有1000μL和50μL规格的枪头适配槽。
所述产物收集模块7的下方设置有温控适配器，用于加速氮吹干燥，上方设置有收集管架15，收集管架15不限于试管和离心管，且收集管架15上面可以分别叠放SPE柱和氮吹模块5，分别用于盛接纯化后的产物和干燥；
在本发明实施例中，优选的，为防止产物在干燥过程中，沾在玻璃试管壁上，造成损失，收集架中放置12个离心管，温控适配器设置温度为80℃；
所述收集管架15包括SPE柱架1501，SPE柱架1501中设置有用于存放SPE柱1502的SPE收集管1503。
所述点样模块8包括左侧设置的小瓶适配器和右边设置的用于芯片固定的芯片适配器，所述芯片适配器底部有温控适配器，用于点样后的快速干燥；
在本发明的一种实施例中，所述小瓶适配器放置12个液相小瓶，芯片适配器固定一块MADLI芯片，温控适配器设置温度80℃。
所述试剂存储模块9包括保温外壳909和设置于保温外壳909底部的半导体制冷片905，保温外壳909与半导体制冷片905之间设置有冷传导板908，所述保温外壳909的上部安装有用于存放多个容器的上制冷适配器907；
在本发明实施例中，所述保温外壳909上还安装有用于温度测量的温度传感器911；
在本发明又一实施例中，所述半导体制冷片905的下方设置有风扇支架902，风扇支架902上安装有风扇901，半导体制冷片905与风扇901之间还设置有铜散热器903和导热板904，半导体制冷片905制冷过程中的热量传导至导热板904，由铜散热器903和风扇901进行散热。
所述固振荡温控模块11包括振荡器上板1010、偏心轴1005和振荡电机1001，所述振荡器上板1010的下方固定安装有主机连板1007，所述振荡电机1001通过振荡电机座1002固定安装于主机连板1007底部，振荡电机1001的输出端与偏心轴1005固定连接；
在本发明实施例中，所述振荡器上板1010连接安装有收集管适配器1012，收集管适配器1012上放置有若干由振动轴1005带动振荡的收集管1014；所述收集管适配器1012通过销钉固定板Ⅱ1011与振荡器上板1010连接；
在本发明又一实施例中，所述振荡电机1001的输出端与偏心轴1005的连接处还设置有与偏心轴1005适配的平衡块1006和回位弹簧1004；
由收集管适配器1012固定一定数量的收集管1014，用于样品与各种溶液的反应，固振荡温控模块11为样品的反应提供温度与振荡，实现实验流程中温度的快速升降，缩短反应时间，优选的，收集管适配器1012固定12个2mL收集管1014，实验先升温65℃振荡孵育样品，使蛋白高温变性，分子结构松散，再降温，适宜的温度下加入蛋白酶振荡孵育完成酶解反应。
所述样品模块12包括有样品架下板1201和样品架上板1203，样品架下板1201和样品架上板1203由支柱1202连接固定，用于放置样品试管1204。
需要说明的是，在本发明实施例中，所述工作盘位上的元件，包括正压模块4、氮吹模块5、枪头放置模块6、产物收集模块7、点样模块8、试剂存储模块9、固相萃取模块 10、振荡温控模块11和样品模块12，其数量可根据应用情况进行增减，正压模块4、氮吹模块5、枪头放置模块6、产物收集模块7、点样模块8、试剂存储模块9、固相萃取模块10、振荡温控模块11和样品模块12呈九宫格方式排放，这样即将工作区相对独立分开，避免样品区、试剂区和固相萃取区等区域相互污染，又可最大化节约工作基座空间。
在本发明又一实施例中，所述工作平台1垂直面上设置有用于带动移盘爪3进行位置调节的机械臂组件，机械臂组件包括驱动移盘爪3沿x轴运动的x轴机械臂103、驱动移盘爪3沿y轴运动的y轴机械臂104和驱动移盘爪3沿z轴运动的z轴机器臂105。
一种用于固相萃取及芯片阵列点样的液体工作站的使用方法，包括以下步骤：
S1、先提前准备试剂与耗材(试剂A、试剂B、试剂C、试剂D、试剂E、试剂F、试剂G、试剂H、12个SPE柱、12个待处理的血清样品、50ul和1000ul枪头、12个2mL离心管、12个1.5mL液相小瓶、1块MADLI芯片)，手动放置试剂到相应的位置；
枪头放置模块放置1000ul和50ul规格的枪头；产物收集模块放置12个2mL离心管，固相萃取模块放置12个1mLSPE柱；点样模块分别放置12个液相小瓶和MADLI芯片；。
振荡温控模块11放置12个2mL离心管；样品模块12放置12个含待处理血清的采血管；
S2、将机械臂组件上的液体喷加器分别到固相萃取模块10的废液位进行打试剂操作，令试剂灌满管路；
机械臂组件从枪头放置模块取50μL枪头到采血管中取上清30μL血清加入振荡温控模块上的离心管，机械臂组件至枪头废弃槽脱枪头；
机械臂组件从枪头放置模块取50μL枪头到试剂储存模块取30μL A试剂加入离心管适配器中的离心管，升温至65℃，1500rmp 5min后，温度降至45℃，机械臂组件从枪头放置模块取50μL枪头到试剂储存模块取4μL B试剂加入离心管适配器中的离心管，45℃1500rmp振荡20min；在振荡温控模块振荡20min的同时，机械臂组件将固相萃取架移动至固相萃取模块；试剂喷加器到固相萃取架中的SPE柱加入1mL E试剂；机械臂组件从正压模块取正压头至固相萃取柱架上加压过柱；机械臂组件将正压头移回正压模块；试剂喷 加器到SPE固相萃取架加1mL G试剂；机械臂组件从正压模块取正压头至SPE固相萃取架加压过柱；机械臂组件将正压头移回正压模块；试剂喷加器到SPE萃取柱架加1mL E试剂；机械臂组件从正压模块取正压头至SPE萃取柱架加压过柱；机械臂组件将正压头移回正压模块；机械臂组件从枪头放置模块取50μL枪头到试剂储存模块取5μL C试剂加入离心管适配器离心管；试剂喷加器到离心管适配器加540μL E试剂，试剂喷加器到离心管适配器加100μL F试剂；机械臂组件从枪头放置模块取1000μL枪头到离心管适配器取709μL混合样品到SPE萃取柱架上样；机械臂组件从正压模块取正压头至SPE萃取柱架加压过柱；
机械臂组件将正压头移回正压模块；试剂喷加器1至SPE萃取柱架加1mLE试剂,机械臂组件从正压模块取正压头至SPE萃取柱架加压过柱，机械臂组件将正压头移回正压模块，重复此步骤2次；机械臂组件将SPE萃取柱架叠放至产物收集架上；试剂喷加器3至SPE萃取柱架加1mL G试剂；机械臂组件从正压模块取正压头至SPE萃取柱架加压过柱；机械臂组件将正压头移回正压模块；机械臂组件将产物收集适配器上的SPE萃取架至固相萃取柱模块；机械爪将氮吹头移至离心管适配器上方，80℃氮吹，每隔一定时间氮吹头下降，直至产物被吹干；机械臂组件将氮吹头移回氮吹模块；收集架模块下的温控组件降温至25-37℃；试剂喷加器至离心管适配器加入15μLE试剂；机械臂组件从枪头放置模块取枪头至离心管，上下吸喷混匀，将所有产物液体移至液相小瓶；芯片适配器下的温控组件加热至80℃；机械臂组件丛枪头放置模块取枪头至液相小瓶取2ul产物微阵列点样至MADLI芯片；实验最后从点样模块上取出的液相小瓶产物和MADLI芯片可进行液相和飞行质谱的测定，分析样品成分，可用于诊断。
以上的仅是本发明的优选实施方式，应当指出，对于本领域的技术人员来说，在不脱离本发明构思的前提下，还可以作出若干变形和改进，这些也应该视为本发明的保护范围，这些都不会影响本发明实施的效果和专利的实用性。

Claims (10)

  1. 一种用于固相萃取及芯片阵列点样的液体工作站，其特征在于，包括L型的工作平台(1)、若干设置于工作平台(1)水平面上的工作盘位和对工作盘位进行抓取的移盘爪(3)，所述工作盘位包括有正压模块(4)、氮吹模块(5)、枪头放置模块(6)、产物收集模块(7)、点样模块(8)、试剂存储模块(9)、固相萃取模块(10)、振荡温控模块(11)和样品模块(12)，其中：
    所述正压模块(4)包括正压头主件(41)和正压头座(47)，用于对SPE柱进行加压，所述正压头主件(41)的下部与正压头座(47)固定连接，正压头座(47)上下两端开口、内部中空；所述正压头主件(41)的上部固定安装有弯管接头(44)，弯管接头(44)的一端与管道连通、弯管接头(44)的另一端与正压头主件(41)连通，所述管道与空气压缩机连通；所述正压头主件(41)与正压头座(47)之间安装有正压密封圈(45)和密封圈固定板(46)，密封圈固定板(46)上开设有若干供气体流动的通孔；
    所述氮吹模块(5)包括氮吹座(51)和若干固定安装于氮吹座(51)底部的氮吹针(52)，所述氮吹座(51)内部中空，氮吹针(52)与氮吹座(51)连通，氮吹座(51)顶部安装有密封盖(55)，氮吹座(51)的侧边处连通有氮气快接头(54)；
    所述产物收集模块(7)的下方设置有温控适配器，上方设置有收集管架(15)，收集管架(15)不限于试管和离心管，且收集管架(15)上面可以分别叠放SPE柱和氮吹模块(5)；
    所述收集管架(15)包括SPE柱架(1501)，SPE柱架(1501)中设置有用于存放SPE柱(1502)的SPE收集管(1503)；
    所述点样模块(8)包括右侧设置的小瓶适配器和右边设置的用于芯片固定的芯片适配器，所述芯片适配器底部有温控适配器；
    所述试剂存储模块(9)包括保温外壳(909)和设置于保温外壳(909)底部的半导体制冷片(905)，保温外壳(909)与半导体制冷片(905)之间设置有冷传导板(908)，所述保温外壳(909)的上部安装有用于存放多个容器的上制冷适配器(907)；
    所述固振荡温控模块(11)包括振荡器上板(1010)、偏心轴(1005)和振荡电机(1001)， 所述振荡器上板(1010)的下方固定安装有主机连板(1007)，所述振荡电机(1001)通过振荡电机座(1002)固定安装于主机连板(1007)底部，振荡电机(1001)的输出端与偏心轴(1005)固定连接。
  2. 根据权利要求1所述的用于固相萃取及芯片阵列点样的液体工作站，其特征在于，所述正压头主件(41)上部的左右两侧对称安装有两块销钉固定板Ⅰ，销钉固定板Ⅰ上固定安装有若干用于对正压头主件(41)进行固定的销钉Ⅰ(42)。
  3. 根据权利要求1所述的用于固相萃取及芯片阵列点样的液体工作站，其特征在于，所述氮气快接头(54)与氮气瓶连通，所述氮吹针(52)设置的数量为12个。
  4. 根据权利要求3所述的用于固相萃取及芯片阵列点样的液体工作站，其特征在于，所述氮吹座(51)与密封盖(55)的连接处设置有氮吹密封圈(53)。
  5. 根据权利要求4所述的用于固相萃取及芯片阵列点样的液体工作站，其特征在于，所述半导体制冷片(905)的下方设置有风扇支架(902)，风扇支架(902)上安装有风扇(901)，半导体制冷片(905)与风扇(901)之间还设置有铜散热器(903)和导热板(904)。
  6. 根据权利要求1所述的用于固相萃取及芯片阵列点样的液体工作站，其特征在于，所述振荡器上板(1010)连接安装有收集管适配器(1012)，收集管适配器(1012)上放置有若干由振动轴(1005)带动振荡的收集管(1014)；所述收集管适配器(1012)通过销钉固定板Ⅱ(1011)与振荡器上板(1010)连接。
  7. 根据权利要求6所述的用于固相萃取及芯片阵列点样的液体工作站，其特征在于，所述振荡电机(1001)的输出端与偏心轴(1005)的连接处还设置有与偏心轴(1005)适配的平衡块(1006)和回位弹簧(1004)。
  8. 根据权利要求1所述的用于固相萃取及芯片阵列点样的液体工作站，其特征在于，所述样品模块(12)包括有样品架下板(1201)和样品架上板(1203)，样品架下板(1201)和样品架上板(1203)由支柱(1202)连接固定。
  9. 根据权利要求1所述的用于固相萃取及芯片阵列点样的液体工作站，其特征在于，正压模块(4)、氮吹模块(5)、枪头放置模块(6)、产物收集模块(7)、点样模块(8)、 试剂存储模块(9)、固相萃取模块(10)、振荡温控模块(11)和样品模块(12)呈九宫格方式排放。
  10. 根据权利要求1-9任一所述的用于固相萃取及芯片阵列点样的液体工作站，其特征在于，所述工作平台(1)垂直面上设置有用于带动移盘爪(3)进行位置调节的机械臂组件，机械臂组件包括驱动移盘爪(3)沿x轴运动的x轴机械臂(103)、驱动移盘爪(3)沿y轴运动的y轴机械臂(104)和驱动移盘爪(3)沿z轴运动的z轴机器臂(105)。
PCT/CN2022/074360 2021-02-02 2022-01-27 一种用于固相萃取及芯片阵列点样的液体工作站 WO2022166766A1 (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116448535A (zh) * 2023-06-16 2023-07-18 杭州凯莱谱质造科技有限公司 一种全自动磁性固相萃取设备

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112858540A (zh) * 2021-02-02 2021-05-28 药明激创(佛山)生物科技有限公司 一种用于固相萃取及芯片阵列点样的液体工作站

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103230682A (zh) * 2013-04-16 2013-08-07 华南理工大学 一种带氮气保护与负压真空的固相萃取装置
US20130323138A1 (en) * 2012-05-31 2013-12-05 Biolytic Lab Performance, Inc. System for performing automated solid phase extractions
WO2017024899A1 (zh) * 2015-08-11 2017-02-16 广州康昕瑞基因健康科技有限公司 一种试剂工作站
CN206566549U (zh) * 2017-03-09 2017-10-20 北京普立泰科仪器有限公司 全自动固相萃取净化系统
WO2018049926A1 (zh) * 2016-09-19 2018-03-22 清华大学 自动液体处理系统
US20180149669A1 (en) * 2014-01-17 2018-05-31 Hamilton Company Automated Positive Pressure Solid Phase Extraction Apparatus and Method Comprising a Reagent Dispenser Apparatus and Method
CN209387599U (zh) * 2019-01-18 2019-09-13 河南师范大学 一种固相萃取氮吹装置
CN110333364A (zh) * 2019-07-17 2019-10-15 中国科学院生态环境研究中心 一种全自动生物评价和化学分析一体机及方法
CN111624277A (zh) * 2020-04-07 2020-09-04 苏州艾迪迈医疗科技有限公司 一种全自动样本综合前处理仪
CN211896907U (zh) * 2020-01-22 2020-11-10 北京和利康源医疗科技有限公司 一种多功能液体工作站
CN112210476A (zh) * 2020-11-12 2021-01-12 药明激创(佛山)生物科技有限公司 一种应用于生物学的液体工作站
CN112858540A (zh) * 2021-02-02 2021-05-28 药明激创(佛山)生物科技有限公司 一种用于固相萃取及芯片阵列点样的液体工作站
CN215375258U (zh) * 2021-02-02 2021-12-31 药明激创(佛山)生物科技有限公司 一种用于固相萃取及芯片阵列点样的液体工作站

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130323138A1 (en) * 2012-05-31 2013-12-05 Biolytic Lab Performance, Inc. System for performing automated solid phase extractions
CN103230682A (zh) * 2013-04-16 2013-08-07 华南理工大学 一种带氮气保护与负压真空的固相萃取装置
US20180149669A1 (en) * 2014-01-17 2018-05-31 Hamilton Company Automated Positive Pressure Solid Phase Extraction Apparatus and Method Comprising a Reagent Dispenser Apparatus and Method
WO2017024899A1 (zh) * 2015-08-11 2017-02-16 广州康昕瑞基因健康科技有限公司 一种试剂工作站
WO2018049926A1 (zh) * 2016-09-19 2018-03-22 清华大学 自动液体处理系统
CN206566549U (zh) * 2017-03-09 2017-10-20 北京普立泰科仪器有限公司 全自动固相萃取净化系统
CN209387599U (zh) * 2019-01-18 2019-09-13 河南师范大学 一种固相萃取氮吹装置
CN110333364A (zh) * 2019-07-17 2019-10-15 中国科学院生态环境研究中心 一种全自动生物评价和化学分析一体机及方法
CN211896907U (zh) * 2020-01-22 2020-11-10 北京和利康源医疗科技有限公司 一种多功能液体工作站
CN111624277A (zh) * 2020-04-07 2020-09-04 苏州艾迪迈医疗科技有限公司 一种全自动样本综合前处理仪
CN112210476A (zh) * 2020-11-12 2021-01-12 药明激创(佛山)生物科技有限公司 一种应用于生物学的液体工作站
CN112858540A (zh) * 2021-02-02 2021-05-28 药明激创(佛山)生物科技有限公司 一种用于固相萃取及芯片阵列点样的液体工作站
CN215375258U (zh) * 2021-02-02 2021-12-31 药明激创(佛山)生物科技有限公司 一种用于固相萃取及芯片阵列点样的液体工作站

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116448535A (zh) * 2023-06-16 2023-07-18 杭州凯莱谱质造科技有限公司 一种全自动磁性固相萃取设备
CN116448535B (zh) * 2023-06-16 2023-11-10 杭州凯莱谱质造科技有限公司 一种全自动磁性固相萃取设备

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